Microscopy. Matti Hotokka Department of Physical Chemistry Åbo Akademi University
|
|
|
- Elizabeth Eaton
- 5 years ago
- Views:
Transcription
1 Microscopy Matti Hotokka Department of Physical Chemistry Åbo Akademi University
2 What s coming Anatomy of a microscope Modes of illumination Practicalities Special applications
3 Basic microscope Ocular Objective Sample stage Condenser lens Light source
4 Parts of a microscope
5 Modern microscope microscopy.fsu.edu/primer/
6 Types of microscopes Stereo microscope Preparation microscope Biological microscope Ordinary microscope
7 Stereo microscopy Magnification typically x Three-dimensional image The sample can be almost anything Large working area
8 Ordinary microscopy Magnification x Two-dimensional Sample on a microscope glass Small working area
9 Elementary optics Magnification of a lens b M = b f f 1 f 1 Real image
10 Elementary optics Magnification of a lens m = 1+ d f d For a normally accomodated eye d = 25 cm Imaginary image f 2 f 2
11 Elementary optics Magnification = Mxm = Optical length = approx. b b Microscope f 1 f 2
12 Numerical aperture Let be the half-angle of the cone of light that can enter the lens. Let n be the refractive index of the surrounding medium (1 for air, 1.5 for oil). The the numerical aperture NA=n sin
13 f-number For small numerical apertures f 1 2NA
14 Typical objectives Magnification NA 4x x x x (dry) x (immersed) 1.25 Dry NA=0.4 Immersion media Water, n=1.33 Glycerin, n=1.47 Oil, n=1.51 Most objectives with magnification 60x or higher are designed to allow immersion in oil. Do not use other immersion media since the lenses in the objective are glued together. The glue may be affected by other liquids. Immersed in oil NA=0.8
15 Resolution Two objects are seen separately if their distance is larger than the Airy radius r = 122. λ Airy 2NA r
16 Resolution 1.25 NA r=0.61/na =550 nm Magnification NA m 4x x x x (dry) x (immersed) m
17 Depth of field The higher the numerical aperture the smaller depth is in focus. Magnification NA m 4x x x x (dry) x (immersed) Nearest plane in focus Farthest plane in focus
18 Field number Field of view diameter The diameter of the visible region is (Ø mm) N P = M q Obj N = The field number of the ocular, e.g., 10/20 (magnification/n) M obj = Magnification of the objective, e.g., 40/0.65 (magnification / NA) q = The tubus, usually 1, sometimes 1.25 In the example, diameter of the visible part of the sample is 20/40 mm = 0.5 mm
19 Methods in microscopy Transmittance illunimation Bright field Dark field Polarization Phase contrast Interference contrast Reflectance illumination A similar list
20 Bright field Objective Stained sample Stage Condenser
21 Dark field Light scattered from the sample Objective Unstained sample Stage Condenser Shade Good for samples in water.
22 Phase contrast Useful for large magnifications (400 x ->) Useful when the sample is colorless or the details are so fine that color does not show in bright field.
23 Interference contrast DIC Thicker samples than usual can be observed in focus.
24 Polarization Tartaric acid crystals
25 Digital photography The photograph shows approx. 60 % of the field visible in the ocular
26 Practicalities All knobs that can be turned with fingers without tools may (and should) be turned. Otherwise the grease will harden. Clean Adjust Set Köhler illumination
27 Clean the objectives Blow the dust away Use clean and dry pressure air Wash the objective Use a solution of 70 % ether, 30 % alcohol Circular motions Inspect visually (use ocular)
28 Adjust eyepieces Adjust focus Close right eye Adjust carefully focus so that the image is sharp Adjust accomodation Close left eye instead Turn the accomodation ring to make the image sharp
29 Köhler illumination Maximize the illumination of the sample Use a 10x objective. Focus to the sample. Move the condenser lens to uppermost position. Close the field aperture. Move the condenser lens down a bit to make the edge of the aperture sharp. Move the illuminated dot to the center of the visible field. Open the field aperture until the aperture edge is just outside the visible field.
30 Special applications Confocal microscopy Fluorescence microscopy Near-field microscopy Image analysis
31 Confocal microscopy Marvin Minsky 1955 Laser Detector Beamsplitter Pinhole Good depth profile. Crisp images. d z Focal plane
32 Fluorescence microscopy Detector Fluorescence filter Dichroic mirror Hg lamp Objective Excitation filter Sample
33 Fluorescence microscope
34 Fluorescence microscopy Topology: shear force; transmission, fluorescence: SNOM. Phase separated polymer sample.
35 Near-field microscopy Only intensity is measured. Position of the tip maps the surface Reflection y 10 nm x Transmission
36 Image analysis
Imaging Introduction. September 24, 2010
Imaging Introduction September 24, 2010 What is a microscope? Merriam-Webster: an optical instrument consisting of a lens or combination of lenses for making enlarged images of minute objects; especially:
Resolution. Diffraction from apertures limits resolution. Rayleigh criterion θ Rayleigh = 1.22 λ/d 1 peak at 2 nd minimum. θ f D
Microscopy Outline 1. Resolution and Simple Optical Microscope 2. Contrast enhancement: Dark field, Fluorescence (Chelsea & Peter), Phase Contrast, DIC 3. Newer Methods: Scanning Tunneling microscopy (STM),
VISUAL PHYSICS ONLINE DEPTH STUDY: ELECTRON MICROSCOPES
VISUAL PHYSICS ONLINE DEPTH STUDY: ELECTRON MICROSCOPES Shortly after the experimental confirmation of the wave properties of the electron, it was suggested that the electron could be used to examine objects
Light Microscopy. Upon completion of this lecture, the student should be able to:
Light Light microscopy is based on the interaction of light and tissue components and can be used to study tissue features. Upon completion of this lecture, the student should be able to: 1- Explain the
Biology 29 Cell Structure and Function Spring, 2009 Springer LABORATORY 1: THE LIGHT MICROSCOPE
Biology 29 Cell Structure and Function Spring, 2009 Springer LABORATORY 1: THE LIGHT MICROSCOPE Prior to lab: 1) Read these instructions (p 1-6) 2) Go through the online tutorial, the microscopy pre-lab
Microscopy: Fundamental Principles and Practical Approaches
Microscopy: Fundamental Principles and Practical Approaches Simon Atkinson Online Resource: http://micro.magnet.fsu.edu/primer/index.html Book: Murphy, D.B. Fundamentals of Light Microscopy and Electronic
Chapter 2 The Study of Microbial Structure: Microscopy and Specimen Preparation
Chapter 2 The Study of Microbial Structure: Microscopy and Specimen Preparation 1 Lenses and the Bending of Light light is refracted (bent) when passing from one medium to another refractive index a measure
Microscopy Techniques that make it easy to see things this small.
Microscopy Techniques that make it easy to see things this small. What is a Microscope? An instrument for viewing objects that are too small to be seen easily by the naked eye. Dutch spectacle-makers Hans
Observing Microorganisms through a Microscope LIGHT MICROSCOPY: This type of microscope uses visible light to observe specimens. Compound Light Micros
PHARMACEUTICAL MICROBIOLOGY JIGAR SHAH INSTITUTE OF PHARMACY NIRMA UNIVERSITY Observing Microorganisms through a Microscope LIGHT MICROSCOPY: This type of microscope uses visible light to observe specimens.
DIC Imaging using Laser Scanning Microscopes (LSM) on Inverted Stands
DIC Imaging using Laser Scanning Microscopes (LSM) on Inverted Stands Differential Interference Contrast (DIC) imaging is a technique used to increase contrast in brightfield images. In confocal systems,
Very short introduction to light microscopy and digital imaging
Very short introduction to light microscopy and digital imaging Hernan G. Garcia August 1, 2005 1 Light Microscopy Basics In this section we will briefly describe the basic principles of operation and
The Compound Microscope. Brightfield: Köhler Illumination
Outline History of Microscopy The Magnifying Glass The Compound Microscope Brightfield: Köhler Illumination Microscopy µικροσ (mikros): small σκοπειν (skopein): to observe History of Microscopy Well :
A BRIEF INTRODUCTION TO MICROSCOPY The two key properties of a microscope that allow you to see microbes are resolution and magnification.
A BRIEF INTRODUCTION TO MICROSCOPY The two key properties of a microscope that allow you to see microbes are resolution and magnification. Magnification refers to the enlargement of the specimen when seen
Observing Microorganisms through a Microscope
2016/2/19 PowerPoint Lecture Presentations prepared by Bradley W. Christian, McLennan Community College CHAPTER 3 Observing Microorganisms through a Microscope 1 Figure 3.2 Microscopes and Magnification.
Basics of Light Microscopy and Metallography
ENGR45: Introduction to Materials Spring 2012 Laboratory 8 Basics of Light Microscopy and Metallography In this exercise you will: gain familiarity with the proper use of a research-grade light microscope
Microscopic Structures
Microscopic Structures Image Analysis Metal, 3D Image (Red-Green) The microscopic methods range from dark field / bright field microscopy through polarisation- and inverse microscopy to techniques like
DIC Imaging using Laser Scanning Microscopes (LSMs) on Axio Imager Stands
DIC Imaging using Laser Scanning Microscopes (LSMs) on Axio Imager Stands Differential Interference Contrast (DIC) imaging is a technique used to increase contrast in brightfield images. In confocal systems,
Katarina Logg, Kristofer Bodvard, Mikael Käll. Dept. of Applied Physics. 12 September Optical Microscopy. Supervisor s signature:...
Katarina Logg, Kristofer Bodvard, Mikael Käll Dept. of Applied Physics 12 September 2007 O1 Optical Microscopy Name:.. Date:... Supervisor s signature:... Introduction Over the past decades, the number
Compound Light Microscopy. Microscopy. Things to remember... 1/22/2017. This is what we use in the laboratory
Compound Light Microscopy This is what we use in the laboratory Microscopy Chapter 3 BIO 440 A series of finely ground lenses is used to form a magnified image Specimen is illuminated with visible light
Figure 3.4 Approximate size of various types of cells. ~10 um. Red Blood Cells = mm 1500 um. Width of penny Pearson Education, Inc.
Figure 3.4 Approximate size of various types of cells. ~10 um Red Blood Cells 1.5mm 1500 um Width of penny = 1500 Figure 4.3 The limits of resolution (and some representative objects within those ranges)
FLUORESCENCE MICROSCOPY. Matyas Molnar and Dirk Pacholsky
FLUORESCENCE MICROSCOPY Matyas Molnar and Dirk Pacholsky 1 The human eye perceives app. 400-700 nm; best at around 500 nm (green) Has a general resolution down to150-300 μm (human hair: 40-250 μm) We need
Operation Guide for the Leica SP2 Confocal Microscope Bio-Imaging Facility Hunter College October 2009
Operation Guide for the Leica SP2 Confocal Microscope Bio-Imaging Facility Hunter College October 2009 Introduction of Fluoresence Confocal Microscopy The first confocal microscope was invented by Princeton
Practical Light Microscopy
Biomedical & X-ray Physics Kjell Carlsson Important: Study the preparatory exercises carefully before the lab session starts! Practical Light Microscopy Laboratory instructions for course SK2500/01, Physics
Microscopy http://www.microscopyu.com/articles/phasecontrast/phasemicroscopy.html http://micro.magnet.fsu.edu/primer/anatomy/anatomy.html 2005, Dr. Jack Ikeda & Dr. Gail Grabner 9 Nikon Labophot (Question
Invitation for a walk through microscopy. Sebastian Schuchmann Jörg Rösner
Invitation for a walk through microscopy Sebastian Schuchmann Jörg Rösner joerg.roesner@charite.de Techniques in microscopy Conventional (light) microscopy bright & dark field, phase & interference contrast
Light microscopy BMB 173, Lecture 14, Feb. 21, 2018
Light microscopy The Structural Biology Continuum Next two lectures: Light microscopy Many slides taken from Scott Fraser, Murphy s Fundamentals of light microscopy, Alberts Molecular Biology of the Cell,
BASICS IN BIOIMAGING AND OPTICS PLATFORM EPFL SV PTBIOP LIGHT MICROSCOPY
BASICS IN LIGHT MICROSCOPY OVERVIEW 1. Motivation 2. Basic in optics 3. How microscope works 4. Illumination and resolution 5. Microscope optics 6. Contrasting methods -2- MOTIVATION Why do we need microscopy?
MICROSCOPY MICROSCOPE TERMINOLOGY
1 MICROSCOPY Most of the microorganisms that we talk about in this class are too small to be seen with the naked eye. The instruments we will use to visualize these microbes are microscopes. The laboratory
Microscope anatomy, image formation and resolution
Microscope anatomy, image formation and resolution Ian Dobbie Buy this book for your lab: D.B. Murphy, "Fundamentals of light microscopy and electronic imaging", ISBN 0-471-25391-X Visit these websites:
Basics of confocal imaging (part I)
Basics of confocal imaging (part I) Swiss Institute of Technology (EPFL) Faculty of Life Sciences Head of BIOIMAGING AND OPTICS BIOP arne.seitz@epfl.ch Lateral resolution BioImaging &Optics Platform Light
Bio 407. Applied microscopy. Introduction into light microscopy. José María Mateos. Center for Microscopy and Image Analysis
Center for Microscopy and Image Analysis Bio 407 Applied Introduction into light José María Mateos Fundamentals of light Compound microscope Microscope composed of an objective and an additional lens (eyepiece,
ANSWER KEY Lab 2 (IGB): Bright Field and Fluorescence Optical Microscopy and Sectioning
Phys598BP Spring 2016 University of Illinois at Urbana-Champaign ANSWER KEY Lab 2 (IGB): Bright Field and Fluorescence Optical Microscopy and Sectioning Location: IGB Core Microscopy Facility Microscope:
Reflection! Reflection and Virtual Image!
1/30/14 Reflection - wave hits non-absorptive surface surface of a smooth water pool - incident vs. reflected wave law of reflection - concept for all electromagnetic waves - wave theory: reflected back
Microscopy Training & Overview
Microscopy Training & Overview Product Marketing October 2011 Stephan Briggs - PLE OVERVIEW AND PRESENTATION FLOW Glossary and Important Terms Introduction Timeline Innovation and Advancement Primary Components
microscopy A great online resource Molecular Expressions, a Microscope Primer Partha Roy
Fundamentals of optical microscopy A great online resource Molecular Expressions, a Microscope Primer http://micro.magnet.fsu.edu/primer/index.html Partha Roy 1 Why microscopy Topics Functions of a microscope
Introduction to Light Microscopy. (Image: T. Wittman, Scripps)
Introduction to Light Microscopy (Image: T. Wittman, Scripps) The Light Microscope Four centuries of history Vibrant current development One of the most widely used research tools A. Khodjakov et al. Major
OPTICAL PRINCIPLES OF MICROSCOPY. Interuniversity Course 28 December 2003 Aryeh M. Weiss Bar Ilan University
OPTICAL PRINCIPLES OF MICROSCOPY Interuniversity Course 28 December 2003 Aryeh M. Weiss Bar Ilan University FOREWORD This slide set was originally presented at the ISM Workshop on Theoretical and Experimental
Tissue Preparation ORGANISM IMAGE TISSUE PREPARATION. 1) Fixation: halts cell metabolism, preserves cell/tissue structure
Lab starts this week! ANNOUNCEMENTS - Tuesday or Wednesday 1:25 ISB 264 - Read Lab 1: Microscopy and Imaging (see Web Page) - Getting started on Lab Group project - Organ for investigation - Lab project
Education in Microscopy and Digital Imaging
Contact Us Carl Zeiss Education in Microscopy and Digital Imaging ZEISS Home Products Solutions Support Online Shop ZEISS International ZEISS Campus Home Interactive Tutorials Basic Microscopy Spectral
TRAINING MANUAL. Multiphoton Microscopy LSM 510 META-NLO
TRAINING MANUAL Multiphoton Microscopy LSM 510 META-NLO September 2010 Multiphoton Microscopy Training Manual Multiphoton microscopy is only available on the LSM 510 META-NLO system. This system is equipped
Lecture 23 MNS 102: Techniques for Materials and Nano Sciences
Lecture 23 MNS 102: Techniques for Materials and Nano Sciences Reference: #1 C. R. Brundle, C. A. Evans, S. Wilson, "Encyclopedia of Materials Characterization", Butterworth-Heinemann, Toronto (1992),
The Nature of Light. Light and Energy
The Nature of Light Light and Energy - dependent on energy from the sun, directly and indirectly - solar energy intimately associated with existence of life -light absorption: dissipate as heat emitted
ML7520 ML7530 DIOPTER ADJUSTMENT RING BINOCULAR BODY, INCLINED 30. (a) Field Iris Control Lever. (c) Filter Slots EYEPIECES, KHW10X
JAPAN DIOPTER ADJUSTMENT RING BINOCULAR BODY, INCLINED 30 (a) Field Iris Control Lever (c) Filter Slots EYEPIECES, KHW10X ANALYZER CONTROL LEVER (b) Aperture Iris Control Lever LIGHT SOURCE HOUSING VERTICAL
Motorized Axio Observer Start-up instructions
Start-up instructions 1. If using fluorescence turn on Fluorescent light source. TL light Source (Hal 100) 2. Turn on microscope using switch on lower left side of the microscope. 3. If imaging, turn on
Nikon Ti-E Microscope Manual. Rightmire Hall Ohio State University. Director: Tony Brown Rightmire
Nikon Ti-E Microscope Manual Rightmire Hall Ohio State University Director: Tony Brown Rightmire 060 292-1205 brown.2302@osu.edu Facility Manager: Paula Monsma Rightmire 062 293-0939 292-1367 monsma.1@osu.edu
MICROSCOPE LAB. Resolving Power How well specimen detail is preserved during the magnifying process.
AP BIOLOGY Cells ACTIVITY #2 MICROSCOPE LAB OBJECTIVES 1. Demonstrate proper care and use of a compound microscope. 2. Identify the parts of the microscope and describe the function of each part. 3. Compare
Chapter 2 Alignment C. Robert Bagnell, Jr., Ph.D., 2012
Chapter 2 Alignment C. Robert Bagnell, Jr., Ph.D., 2012 Figure 2.1 is an image of striated muscle taken with a misaligned microscope and figure 2.2 is with a properly aligned microscope. To the untrained
BASICS OF CONFOCAL IMAGING (PART I)
BASICS OF CONFOCAL IMAGING (PART I) INTERNAL COURSE 2012 LIGHT MICROSCOPY Lateral resolution Transmission Fluorescence d min 1.22 NA obj NA cond 0 0 rairy 0.61 NAobj Ernst Abbe Lord Rayleigh Depth of field
Easy Kohler Illumination Method
Easy Kohler Illumination Method ACADEMIC SKILLS CENTRE (ASC) A. Silverberg Completion of a Kohler illumination method is required before a microscope can be used efficiently. The Kohler method is designed
Therefore, all descriptions and illustrations in this instruction manual, including all specifications are subject to change without notice.
We are constantly endeavouring to improve our instruments and to adapt them to the requirements of modern research techniques and testing methods. This involves modification to the mechanical structure
INTRODUCTION TO OPTICAL MICROSCOPY
Experimental Biophysics TEK265, FYST23, TNF060, FAF010F Lab Exercise Supervisor: Karl Adolfsson Written by Peter Jönsson and Jason Beech Updated by Henrik Persson, Karl Adolfsson and Zhen Li karl.adolfsson@ftf.lth.se
2018 MICROSCOPE REVIEW by Karen L. Lancour RELATIVE SIZE OF MICROBES
2018 MICROSCOPE REVIEW by Karen L. Lancour RELATIVE SIZE OF MICROBES 1000 millimeters (mm) = 1 meter (m) 1000 micrometers (µm or mcm) = 1 millimeter (mm) 1000 nanometers (nm) = 1 micrometer (mcm) Size
Burton's Microbiology for the Health Sciences
Burton's Microbiology for the Health Sciences Chapter 2. Viewing the Microbial World Chapter 2 Outline Introduction Using the metric system to express the sizes of microbes Microscopes Simple microscopes
attocfm I for Surface Quality Inspection NANOSCOPY APPLICATION NOTE M01 RELATED PRODUCTS G
APPLICATION NOTE M01 attocfm I for Surface Quality Inspection Confocal microscopes work by scanning a tiny light spot on a sample and by measuring the scattered light in the illuminated volume. First,
Point Spread Function. Confocal Laser Scanning Microscopy. Confocal Aperture. Optical aberrations. Alternative Scanning Microscopy
Bi177 Lecture 5 Adding the Third Dimension Wide-field Imaging Point Spread Function Deconvolution Confocal Laser Scanning Microscopy Confocal Aperture Optical aberrations Alternative Scanning Microscopy
OPELCO OPtical ELements COrporation LB Objective Series for Biological Use
LB Objective Series for Biological Use 105 Executive Drive Suite 100 Dulles, VA 20166-9558 Tel: (703) 471-0080 S PLAN APOCHROMAT OBJECTIVES These objectives compensate for three wavelength of chromatic
2/4/15. Brightfield Microscopy! It s all about Magnification..! or is it?!
Brightfield Microscopy It s all about Magnification.. or is it? 1 What actually does go into chosing a microscope Choice depends on what you need the microscope to do. Do you want to magnify stained specimens?
The microscope is useful in making observations and collecting data in scientific experiments. Microscopy involves three basic concepts:
AP BIOLOGY Chapter 6 NAME DATE Block MICROSCOPE LAB PART I: COMPOUND MICROSCOPE OBJECTIVES: After completing this exercise you should be able to: Demonstrate proper care and use of a compound microscope.
Operating Instructions for Zeiss LSM 510
Operating Instructions for Zeiss LSM 510 Location: GNL 6.312q (BSL3) Questions? Contact: Maxim Ivannikov, maivanni@utmb.edu 1 Attend A Complementary Training Before Using The Microscope All future users
Laboratory Introduction
Laboratory Introduction There are two basic categories of microscopes: light microscopes and electron microscopes. Light, or optical, microscopes require light waves to provide the illumination while electron
INTRODUCTION THIN LENSES. Introduction. given by the paraxial refraction equation derived last lecture: Thin lenses (19.1) = 1. Double-lens systems
Chapter 9 OPTICAL INSTRUMENTS Introduction Thin lenses Double-lens systems Aberrations Camera Human eye Compound microscope Summary INTRODUCTION Knowledge of geometrical optics, diffraction and interference,
Heisenberg) relation applied to space and transverse wavevector
2. Optical Microscopy 2.1 Principles A microscope is in principle nothing else than a simple lens system for magnifying small objects. The first lens, called the objective, has a short focal length (a
2017 MICROSCOPE REVIEW by Karen L. Lancour RELATIVE SIZE OF MICROBES
2017 MICROSCOPE REVIEW by Karen L. Lancour RELATIVE SIZE OF MICROBES 1000 millimeters (mm) = 1 meter (m) 1000 micrometers (µm or mcm) = 1 millimeter (mm) 1000 nanometers (nm) = 1 micrometer (mcm) Size
Light microscopy. Part II
Light microscopy Part II What is numerical aperature (NA)? Usually, higher magnifica>on objec>ves have greater NAs Sample specifica>ons objective magnification NA working distance (mm) Achromat 10x 0.25
BIOIMAGING AND OPTICS PLATFORM EPFL SV PTBIOP BASICS IN LIGHT MICROSCOPY
BASICS IN LIGHT MICROSCOPY INTERNAL COURSE 2014 13 TH JANUARY OVERVIEW 1. Motivation 2. Basic in optics 3. How microscope works 4. Illumination and resolution 5. Microscope optics 6. Contrasting methods
Microscope. Dr. Leena Barhate Department of Microbiology M.J.College, Jalgaon
Microscope Dr. Leena Barhate Department of Microbiology M.J.College, Jalgaon Acknowledgement http://www.cerebromente.org.br/n17/histor y/neurons1_i.htm Google Images http://science.howstuffworks.com/lightmicroscope1.htm
CCAM s Selection of. Zeiss Microscope Objectives
CCAM s Selection of Zeiss Microscope Objectives 1. Magnification Image scale 2. Resolution The minimum separation distance between two points that are clearly resolved. The resolution of an objective is
Reichert Univar Manual
Reichert Univar Manual Translated from the 11/1975 German language edition, with slight modifications. William R. Porter San Marcos CA USA 2017 v 1.3 Notes This is a very slightly-modified, new (2017)
CCAM Microscope Objectives
CCAM Microscope Objectives Things to consider when selecting an objective Magnification Numerical Aperture (NA) resolving power and light intensity of the objective Working Distance distance between the
Basic Microscopy for Plant Biology
Page 1 of 8 Basic Microscopy for Plant Biology OBJECTIVES After completing this exercise, you should be able to do the following: a. Name the parts of the compound microscope and the functions of each.
Marine Invertebrate Zoology Microscope Introduction
Marine Invertebrate Zoology Microscope Introduction Introduction A laboratory tool that has become almost synonymous with biology is the microscope. As an extension of your eyes, the microscope is one
Microbiology Laboratory 2
Microbiology Laboratory 2 Microscopy Background Microorganisms are too small to be seen with the naked eye. Thus a microscope is used to magnify objects so they can be observed. A lens consists of one
User Manual. Cat.-No /1
User Manual Cat.-No. 16100/1 No. DATE / Rev. REVISION DESCRIPTION 1 01/2004-07 First edition 2 02/2006-08 Addition of Chapter 4.2.1 / Köhler Illumination; Update Specifications i ii 1 INTRODUCTION This
LSM 510 META in Chang Gung University
Content LSM 510 META in Chang ung University LSM 510 META 路 理 The features and applications of LSM 510 META 01-09 Introduction of the hardware 10-12 Fluorescence observation in conventional microscope
Manual for BMS E1 eplan series, compound microscope
Manual for BMS E1 eplan series, compound microscope The compound microscope allows it to study, at cell level, structures of textures of botanical and zoological nature. (e.g. slides of roots, leaves and
Lecture 4 to 5 MICROSCOPY-PRINCIPLES AND TYPES
Lecture 4 to 5 MICROSCOPY-PRINCIPLES AND TYPES Microorganisms are too small to be seen by our unaided eyes and the microscopes are of crucial importance as they help to view the microbes. A microscope
plasmonic nanoblock pair
Nanostructured potential of optical trapping using a plasmonic nanoblock pair Yoshito Tanaka, Shogo Kaneda and Keiji Sasaki* Research Institute for Electronic Science, Hokkaido University, Sapporo 1-2,
Chapter 3 Op,cal Instrumenta,on
Imaging by an Op,cal System Change in curvature of wavefronts by a thin lens Chapter 3 Op,cal Instrumenta,on 3-1 Stops, Pupils, and Windows 3-4 The Camera 3-5 Simple Magnifiers and Eyepieces 1. Magnifiers
Chapter 1 Parts. Figure 1.1. Parts of a Compound Light Microscope
Chapter 1 Parts Chapter 1 Parts Figure 1.1 illustrates the parts of an upright compound microscope and indicates the terminology that I use in these notes. Figure 1.1. Parts of a Compound Light Microscope
User Manual. Digital Compound Binocular LED Microscope. MicroscopeNet.com
User Manual Digital Compound Binocular LED Microscope Model MD82ES10 MicroscopeNet.com Table of Contents i. Caution... 1 ii. Care and Maintenance... 2 1. Components Illustration... 3 2. Installation...
Nikon E800 Operating Instructions.
Nikon E800 Operating Instructions. You can request electronic copies of this manual by contacting lshats@jhsph.edu Copies are also available on the JHU MMI Department web site. Please send your comments
<Chap. 2 Optics> 1.Light directivity. Light directivity can be seen using smoke and milky water in a plastic bottle
1.Light directivity Light directivity can be seen using smoke and milky water in a plastic bottle Laser 3 cm Principle of pinhole camera (γray camera) Object Dark image Eye Ground glass
Match the microscope structures given in the left column with the statements in the right column that identify or describe them.
49 Prelab for Name Match the microscope structures given in the left column with the statements in the right column that identify or describe them. Key: a. coarse adjustment knob f. turret or nosepiece
MICROSCOPY FOR THE DEVELOPMENTAL BIOLOGY STUDENT...
MICROSCOPY FOR THE DEVELOPMENTAL BIOLOGY STUDENT... You will be using two configurations of microscope during the course of the semester to observe specimens and record your results: compound microscopes
Exercise 8: Interference and diffraction
Physics 223 Name: Exercise 8: Interference and diffraction 1. In a two-slit Young s interference experiment, the aperture (the mask with the two slits) to screen distance is 2.0 m, and a red light of wavelength
Examination, TEN1, in courses SK2500/SK2501, Physics of Biomedical Microscopy,
KTH Applied Physics Examination, TEN1, in courses SK2500/SK2501, Physics of Biomedical Microscopy, 2009-06-05, 8-13, FB51 Allowed aids: Compendium Imaging Physics (handed out) Compendium Light Microscopy
Olympus Fluoview 1000S Spectral Confocal Microscope Introduction to the NRI-MCDB Microscopy Facility Spectral Confocal Microscope
Olympus Fluoview 1000S Spectral Confocal Microscope Introduction to the NRI-MCDB Microscopy Facility Spectral Confocal Microscope Improved Optics More Lasers 405 diode 440 diode 488 Argon 515 Argon 559
used for low power magnification of a sample image is 3 dimensional
MICROSCOPES One of the most important inventions in the advancement of Biology 1. Simple Microscopes ie. magnifying glass, stereoscope (dissecting scope) have a single lens or a pair of lenses combined
Applications of Optics
Nicholas J. Giordano www.cengage.com/physics/giordano Chapter 26 Applications of Optics Marilyn Akins, PhD Broome Community College Applications of Optics Many devices are based on the principles of optics
Properties of optical instruments. Visual optical systems part 2: focal visual instruments (microscope type)
Properties of optical instruments Visual optical systems part 2: focal visual instruments (microscope type) Examples of focal visual instruments magnifying glass Eyepieces Measuring microscopes from the
Microscopy. Lecture 2: Optical System of the Microscopy II Herbert Gross. Winter term
Microscopy Lecture 2: Optical System of the Microscopy II 212-1-22 Herbert Gross Winter term 212 www.iap.uni-jena.de Preliminary time schedule 2 No Date Main subject Detailed topics Lecturer 1 15.1. Optical
BASICS IN LIGHT MICROSCOPY
BASICS IN LIGHT MICROSCOPY INTERNAL COURSE 2015 26 TH JANUARY OVERVIEW Light microscopy Why do we need it? How does it work? What are its limitations? What do we need to consider? - 2 - HUMAN EYE Normal
Indian Institute of technology Madras Presents NPTEL NATIONAL PROGRAMME ON TECHNOLOGY ENHANCED LEARNING
Indian Institute of technology Madras Presents NPTEL NATIONAL PROGRAMME ON TECHNOLOGY ENHANCED LEARNING Lecture - 5 Materials Characterization Fundamentals of Optical microscopy Dr. S. Sankaran Associate
Chapter Ray and Wave Optics
109 Chapter Ray and Wave Optics 1. An astronomical telescope has a large aperture to [2002] reduce spherical aberration have high resolution increase span of observation have low dispersion. 2. If two
PHY385H1F Introductory Optics Term Test 2 November 6, 2012 Duration: 50 minutes. NAME: Student Number:.
PHY385H1F Introductory Optics Term Test 2 November 6, 2012 Duration: 50 minutes NAME: Student Number:. Aids allowed: A pocket calculator with no communication ability. One 8.5x11 aid sheet, written on
Components of the Microscope
Swift M3 Microscope The Swift M3 is a versatile microscope designed for both microscopic (high magnification, small field of view) and macroscopic (low magnification, large field of view) applications.
Basic Microscopy. OBJECTIVES After completing this exercise, you should be able to do the following:
Page 1 of 10 Basic Microscopy OBJECTIVES After completing this exercise, you should be able to do the following: a. Name the parts of the compound microscope and the functions of each. b. Describe how
Bandpass Edge Dichroic Notch & More
Edmund Optics BROCHURE Filters COPYRIGHT 217 EDMUND OPTICS, INC. ALL RIGHTS RESERVED 1/17 Bandpass Edge Dichroic Notch & More Contact us for a Stock or Custom Quote Today! USA: +1-856-547-3488 EUROPE:
Chapter 3 Op+cal Instrumenta+on
Chapter 3 Op+cal Instrumenta+on 3-1 Stops, Pupils, and Windows 3-4 The Camera 3-5 Simple Magnifiers and Eyepieces 3-6 Microscopes 3-7 Telescopes Today (2011-09-22) 1. Magnifiers 2. Camera 3. Resolution
Final Exam, 150 points PMB 185: Techniques in Light Microscopy
Final Exam, 150 points Name PMB 185: Techniques in Light Microscopy Point value is in parentheses at the end of each question. Note: GFP = green fluorescent protein ; CFP = cyan fluorescent protein ; YFP