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1 Nanosurf easyscan 2 FlexAFM Your Versatile AFM System for Materials and Life Science

2 The new Nanosurf easyscan 2 FlexAFM scan head makes measurements in liquid as simple as measuring in air. The Nanosurf Available Options easyscan 2 FlexAFM System Perform vibration-free measurements with the Nanosurf isostage: Background image: Lead Tin Selenide. Nanosurf s renowned ease of use has been implemented consistently in the new FlexAFM scan head. The result is the most versatile and flexible AFM ever. Whether you want to operate the FlexAFM in air or in liquid environments, it makes no difference to you as a user. This is true flexibility! Our sample observation optics continuously provide a clear top and side view of the sample and cantilever, regardless of the measuring environment. The quality of our side view in liquid is unique, and is of great help during sample approach. The flexure structure at the basis of the XY-scanner is inherently flat and rugged. Being electromagnetically actuated, all movements in this plane are highly linear. A piezobased Z-scanner provides fast movements in this direction. The patented SureAlign laser optics eliminate laser adjustment once and for all, simply because the laser beam shift that normally occurs upon immersion into liquid is completely absent in the FlexAFM scan head. As a result, you are always working with an optimal laser signal. Optimized for Nanosurf AFMs Fastest setup ever: turn on and go! Calibration grid with 10 µm periodicity, showing the linearity of the FlexAFM. Ten samples from various disciplines No additional preparation tools required A specially designed cantilever holder allows wet samples to be measured in the same manner as dry samples. Changing between the two environments is also a breeze. The choice really is yours! Just place any dry or immersed wet sample under the scan head and start measuring. System setup and operation have remained simple and straightforward. Hook up the scan head to the easyscan 2 controller, connect the USB cable to your computer, start the software, and you re ready to go in minutes! The heart of the FlexAFM system, the Liquid Ready easyscan 2 controller, is as affordable and individually configurable as ever. All easyscan 2 modules and options (including previous easyscan 2 AFM and STM scan heads) are fully compatible, conferring even more versatility to your FlexAFM system. Key Features and Benefits Get the most from your measurement data with the Analysis and Report software: Professional and visually pleasing reports using templates Thiols deposited on a gold surface. Topography image (left). Lateral force map (right). Scan range: 800 nm 800 nm. The FlexAFM scan head on its sample stage. The FlexAFM sample stage provides an easy way to position and approach samples of various thickness and type. Even samples in liquid containers, such as standard Petri dishes, fit on the sample stage and can be accessed by the FlexAFM without any hassle. At the same time, three leveling screws on the FlexAFM scan head maintain the scan head s capability to perform stand-alone measurements without sample stage. The Liquid Ready easyscan 2 controller. In addition to the new FlexAFM scan head, all previous easyscan 2 scan heads can also be operated by this controller, which is fully compatible with all easyscan 2 soft- and hardware. Available in 2009 FlexAFM Micrometer Translation Stage Accurate and reproducible sample positioning Rat-2 fibroblasts imaged using Static mode in a physiological buffer. Cell nuclei and cytoskeletal filaments can be discerned. Scan range: 100 µm 100 µm. Flat, linear, and fast scanning thanks to the flexure-based scanner technology Color view of sample and cantilever Adjustable illumination Air No more laser adjustment because of SureAlign optics 2 FlexAFM Video Camera Option: Easy sample positioning and approach More measurement versatility with the FlexAFM s liquid capabilities and added Lateral Force mode Easy sample positioning and approach thanks to a clear top and side view in air and liquid Quick setup, easy handling, and immediate results because of Nanosurf s ease of use concept and design Easy automation of the data analysis process and simple template creation Particle counting and statistical analysis functions True measurement flexibility in air and in liquid Smart top and side view through the FlexAFM s optical system allow easy sample observation and approach. There is no difference in viewing quality between air and liquid. Teach AFM with ease using the Extended AFM Sample Kit: Manual and tools included The performance of the FlexAFM scan head is also at an entirely new level. The electromagnetically actuated flexure-based scanner technology enables metrology-like applications, even in liquid. It is flat, linear, rugged, and fast! Nanosurf s patented SureAlign technology makes laser adjustment a thing of the past. Whether measuring in air or in liquid, the AFM laser is always right on target. Compact active vibration isolation Bottom view of the Nanosurf easyscan 2 FlexAFM scan head. This image shows the relative location of the scan head leveling screws, the central scanner unit, and the removable cantilever holder. Inverted Microscope Option: Liquid Adapter plates for inverted microscopes The cantilever holder. As central part of the AFM detection system, the cantilever holder contains a cantilever alignment chip for exact cantilever positioning, and all optics related to the SureAlign and Nanosurf top and side view technology. It is magnetically attached to the scanner unit to allow removal from the scan head for easy cleaning and fast cantilever exchange. A removable cantilever spring makes this process even simpler. Measuring in air and in liquid is equally simple, as is shown in the images above. The cantilever holder allows a meniscus to be formed when measuring in a liquid droplet, but it can also dive directly into a liquid layer with up to 6 mm in height. Ability to merge optical microscopy data (e.g. fluorescence images) with AFM images Environment Control Option: DNA adsorbed to muscovite mica and imaged using Dynamic mode in air. Scan range: 250 nm 250 nm. 3 Closed measuring environment Full control of environment parameters (e.g. temperature & atmosphere) 4

3 The new Nanosurf easyscan 2 FlexAFM scan head makes measurements in liquid as simple as measuring in air. The Nanosurf Available Options easyscan 2 FlexAFM System Perform vibration-free measurements with the Nanosurf isostage: Background image: Lead Tin Selenide. Nanosurf s renowned ease of use has been implemented consistently in the new FlexAFM scan head. The result is the most versatile and flexible AFM ever. Whether you want to operate the FlexAFM in air or in liquid environments, it makes no difference to you as a user. This is true flexibility! Our sample observation optics continuously provide a clear top and side view of the sample and cantilever, regardless of the measuring environment. The quality of our side view in liquid is unique, and is of great help during sample approach. The flexure structure at the basis of the XY-scanner is inherently flat and rugged. Being electromagnetically actuated, all movements in this plane are highly linear. A piezobased Z-scanner provides fast movements in this direction. The patented SureAlign laser optics eliminate laser adjustment once and for all, simply because the laser beam shift that normally occurs upon immersion into liquid is completely absent in the FlexAFM scan head. As a result, you are always working with an optimal laser signal. Optimized for Nanosurf AFMs Fastest setup ever: turn on and go! Calibration grid with 10 µm periodicity, showing the linearity of the FlexAFM. Ten samples from various disciplines No additional preparation tools required A specially designed cantilever holder allows wet samples to be measured in the same manner as dry samples. Changing between the two environments is also a breeze. The choice really is yours! Just place any dry or immersed wet sample under the scan head and start measuring. System setup and operation have remained simple and straightforward. Hook up the scan head to the easyscan 2 controller, connect the USB cable to your computer, start the software, and you re ready to go in minutes! The heart of the FlexAFM system, the Liquid Ready easyscan 2 controller, is as affordable and individually configurable as ever. All easyscan 2 modules and options (including previous easyscan 2 AFM and STM scan heads) are fully compatible, conferring even more versatility to your FlexAFM system. Key Features and Benefits Get the most from your measurement data with the Analysis and Report software: Professional and visually pleasing reports using templates Thiols deposited on a gold surface. Topography image (left). Lateral force map (right). Scan range: 800 nm 800 nm. The FlexAFM scan head on its sample stage. The FlexAFM sample stage provides an easy way to position and approach samples of various thickness and type. Even samples in liquid containers, such as standard Petri dishes, fit on the sample stage and can be accessed by the FlexAFM without any hassle. At the same time, three leveling screws on the FlexAFM scan head maintain the scan head s capability to perform stand-alone measurements without sample stage. The Liquid Ready easyscan 2 controller. In addition to the new FlexAFM scan head, all previous easyscan 2 scan heads can also be operated by this controller, which is fully compatible with all easyscan 2 soft- and hardware. Available in 2009 FlexAFM Micrometer Translation Stage Accurate and reproducible sample positioning Rat-2 fibroblasts imaged using Static mode in a physiological buffer. Cell nuclei and cytoskeletal filaments can be discerned. Scan range: 100 µm 100 µm. Flat, linear, and fast scanning thanks to the flexure-based scanner technology Color view of sample and cantilever Adjustable illumination Air No more laser adjustment because of SureAlign optics 2 FlexAFM Video Camera Option: Easy sample positioning and approach More measurement versatility with the FlexAFM s liquid capabilities and added Lateral Force mode Easy sample positioning and approach thanks to a clear top and side view in air and liquid Quick setup, easy handling, and immediate results because of Nanosurf s ease of use concept and design Easy automation of the data analysis process and simple template creation Particle counting and statistical analysis functions True measurement flexibility in air and in liquid Smart top and side view through the FlexAFM s optical system allow easy sample observation and approach. There is no difference in viewing quality between air and liquid. Teach AFM with ease using the Extended AFM Sample Kit: Manual and tools included The performance of the FlexAFM scan head is also at an entirely new level. The electromagnetically actuated flexure-based scanner technology enables metrology-like applications, even in liquid. It is flat, linear, rugged, and fast! Nanosurf s patented SureAlign technology makes laser adjustment a thing of the past. Whether measuring in air or in liquid, the AFM laser is always right on target. Compact active vibration isolation Bottom view of the Nanosurf easyscan 2 FlexAFM scan head. This image shows the relative location of the scan head leveling screws, the central scanner unit, and the removable cantilever holder. Inverted Microscope Option: Liquid Adapter plates for inverted microscopes The cantilever holder. As central part of the AFM detection system, the cantilever holder contains a cantilever alignment chip for exact cantilever positioning, and all optics related to the SureAlign and Nanosurf top and side view technology. It is magnetically attached to the scanner unit to allow removal from the scan head for easy cleaning and fast cantilever exchange. A removable cantilever spring makes this process even simpler. Measuring in air and in liquid is equally simple, as is shown in the images above. The cantilever holder allows a meniscus to be formed when measuring in a liquid droplet, but it can also dive directly into a liquid layer with up to 6 mm in height. Ability to merge optical microscopy data (e.g. fluorescence images) with AFM images Environment Control Option: DNA adsorbed to muscovite mica and imaged using Dynamic mode in air. Scan range: 250 nm 250 nm. 3 Closed measuring environment Full control of environment parameters (e.g. temperature & atmosphere) 4

4 The new Nanosurf easyscan 2 FlexAFM scan head makes measurements in liquid as simple as measuring in air. The Nanosurf Available Options easyscan 2 FlexAFM System Perform vibration-free measurements with the Nanosurf isostage: Background image: Lead Tin Selenide. Nanosurf s renowned ease of use has been implemented consistently in the new FlexAFM scan head. The result is the most versatile and flexible AFM ever. Whether you want to operate the FlexAFM in air or in liquid environments, it makes no difference to you as a user. This is true flexibility! Our sample observation optics continuously provide a clear top and side view of the sample and cantilever, regardless of the measuring environment. The quality of our side view in liquid is unique, and is of great help during sample approach. The flexure structure at the basis of the XY-scanner is inherently flat and rugged. Being electromagnetically actuated, all movements in this plane are highly linear. A piezobased Z-scanner provides fast movements in this direction. The patented SureAlign laser optics eliminate laser adjustment once and for all, simply because the laser beam shift that normally occurs upon immersion into liquid is completely absent in the FlexAFM scan head. As a result, you are always working with an optimal laser signal. Optimized for Nanosurf AFMs Fastest setup ever: turn on and go! Calibration grid with 10 µm periodicity, showing the linearity of the FlexAFM. Ten samples from various disciplines No additional preparation tools required A specially designed cantilever holder allows wet samples to be measured in the same manner as dry samples. Changing between the two environments is also a breeze. The choice really is yours! Just place any dry or immersed wet sample under the scan head and start measuring. System setup and operation have remained simple and straightforward. Hook up the scan head to the easyscan 2 controller, connect the USB cable to your computer, start the software, and you re ready to go in minutes! The heart of the FlexAFM system, the Liquid Ready easyscan 2 controller, is as affordable and individually configurable as ever. All easyscan 2 modules and options (including previous easyscan 2 AFM and STM scan heads) are fully compatible, conferring even more versatility to your FlexAFM system. Key Features and Benefits Get the most from your measurement data with the Analysis and Report software: Professional and visually pleasing reports using templates Thiols deposited on a gold surface. Topography image (left). Lateral force map (right). Scan range: 800 nm 800 nm. The FlexAFM scan head on its sample stage. The FlexAFM sample stage provides an easy way to position and approach samples of various thickness and type. Even samples in liquid containers, such as standard Petri dishes, fit on the sample stage and can be accessed by the FlexAFM without any hassle. At the same time, three leveling screws on the FlexAFM scan head maintain the scan head s capability to perform stand-alone measurements without sample stage. The Liquid Ready easyscan 2 controller. In addition to the new FlexAFM scan head, all previous easyscan 2 scan heads can also be operated by this controller, which is fully compatible with all easyscan 2 soft- and hardware. Available in 2009 FlexAFM Micrometer Translation Stage Accurate and reproducible sample positioning Rat-2 fibroblasts imaged using Static mode in a physiological buffer. Cell nuclei and cytoskeletal filaments can be discerned. Scan range: 100 µm 100 µm. Flat, linear, and fast scanning thanks to the flexure-based scanner technology Color view of sample and cantilever Adjustable illumination Air No more laser adjustment because of SureAlign optics 2 FlexAFM Video Camera Option: Easy sample positioning and approach More measurement versatility with the FlexAFM s liquid capabilities and added Lateral Force mode Easy sample positioning and approach thanks to a clear top and side view in air and liquid Quick setup, easy handling, and immediate results because of Nanosurf s ease of use concept and design Easy automation of the data analysis process and simple template creation Particle counting and statistical analysis functions True measurement flexibility in air and in liquid Smart top and side view through the FlexAFM s optical system allow easy sample observation and approach. There is no difference in viewing quality between air and liquid. Teach AFM with ease using the Extended AFM Sample Kit: Manual and tools included The performance of the FlexAFM scan head is also at an entirely new level. The electromagnetically actuated flexure-based scanner technology enables metrology-like applications, even in liquid. It is flat, linear, rugged, and fast! Nanosurf s patented SureAlign technology makes laser adjustment a thing of the past. Whether measuring in air or in liquid, the AFM laser is always right on target. Compact active vibration isolation Bottom view of the Nanosurf easyscan 2 FlexAFM scan head. This image shows the relative location of the scan head leveling screws, the central scanner unit, and the removable cantilever holder. Inverted Microscope Option: Liquid Adapter plates for inverted microscopes The cantilever holder. As central part of the AFM detection system, the cantilever holder contains a cantilever alignment chip for exact cantilever positioning, and all optics related to the SureAlign and Nanosurf top and side view technology. It is magnetically attached to the scanner unit to allow removal from the scan head for easy cleaning and fast cantilever exchange. A removable cantilever spring makes this process even simpler. Measuring in air and in liquid is equally simple, as is shown in the images above. The cantilever holder allows a meniscus to be formed when measuring in a liquid droplet, but it can also dive directly into a liquid layer with up to 6 mm in height. Ability to merge optical microscopy data (e.g. fluorescence images) with AFM images Environment Control Option: DNA adsorbed to muscovite mica and imaged using Dynamic mode in air. Scan range: 250 nm 250 nm. 3 Closed measuring environment Full control of environment parameters (e.g. temperature & atmosphere) 4

5 The Nanosurf easyscan 2 Controller Specifications in Combination with a FlexAFM Scan Head Nanosurf easyscan 2 Controller Controller Module(s) required: AFM Basic AFM Dynamic Mode Extension Imaging Basic modes Static Force (Contact): Constant Force, Constant Height. Dynamic modes Dynamic Force (Intermittent Contact, etc.): Constant Amplitude, Constant Height. Extended modes Data points Scan modes Rotation and tilt Data display Spreading Resistance, Lateral Force. Force Modulation, Phase Contrast, Magnetic/Electrostatic Force. Up to points (individual width/height) Forward & backward scan / frame up, down, or continuous 0 360, hardware X/Y-slope compensation User definable profile and color map charts for all channels Spectroscopy Basic modes Force-Distance, Force-Voltage Dynamic modes Amplitude-Distance Extended modes Phase-Distance, Current-Voltage, Current-Distance,... Measurement positions Single X/Y point measurement or multiple measurements Data points Up to 2048 points (up to 1024 averages) Lithography (through optional software module) Automated drawing Vector graphic or bitmap graphic import Manual drawing Freehand drawing / Real-time tip movement with mouse Static Force Lithography (multi-pass Lithography with re-imaging) Lithography modes Dynamic Force Lithography (multi-pass Lithography with re-imaging) Accessible parameters Tip speed, Setpoint, Tip voltage, Amplitude,... Number of vectors Unlimited Number of layers 255 CAD vector drawing Supported CAD formats Scripting interface Visualization Special CAD program included GDS II, DXF, OASIS, CIF Allows special user created drawing modes Graphical preview of objects, Object moving, Object scaling, Real-time plot during drawing General Tip potential (resolution) ± 10 V (5 mv) Tip current (resolution) ± 100 µa (3 na) Dynamic freq. range (res.) khz (< 0.1 Hz) Phase Contrast range (res.) ± 90 (< 0.05 ) Phase reference range Computer requirements Windows 2000/XP/Vista (32-bit), USB 2.0 Electronics size / weight Power supply mm / 2.4 kg V AC, 50/60 Hz, 100 W Other products compatible with the Liquid Ready easyscan 2 controller The easyscan 2 STM scan heads The easyscan 2 AFM scan heads 5

6 Nanosurf easyscan 2 FlexAFM Scan Head and Options Specifications and Features Specifications Sample size Maximum Petri dish height (fluid level) Manual approach range Automatic approach range Unlimited without sample stage 100 mm on sample stage 9 mm (6 mm) 30 mm 1.1 mm Maximum scan range 100 µm (1) 10 µm (1) Maximum Z-range 10 µm (1) 3 µm (1) Drive Z-resolution nm (2) nm (2) Drive XY-resolution nm (2) nm (2) XY-linearity mean error < 0.1% XY-flatness at maximum scan range typ. 5 nm typ. 1 nm Z-measurement noise level (RMS, Static Mode in air) typ. 0.3 nm typ nm Z-measurement noise level (RMS, Dynamic Mode in air) typ nm typ nm Scan head dimensions Scan head weight (1) Manufacturing tolerances are ± 5% (2) Calculated by dividing the maximum range by 16 bits mm 1.25 kg Features General design Cantilever alignment Laser adjustment Electrical connection to tip Sample observation Sample illumination Cantilever holder Operating modes Tripod stand-alone, flexure-based electromagnetically actuated XY-scanner, piezo-based Z-scanner Automatic alignment for cantilevers with alignment grooves. Manual laser adjustment possible for special cantilevers. No laser adjustment required upon immersion of cantilever into liquid because of patented SureAlign laser optics Available Top and side view in air and liquid White axial illumination for top and side view. Transmission illumination with illuminated sample holder. Cleanable and replaceable cantilever spring Static Force Lateral Force Dynamic Force Phase Contrast MFM / EFM Spreading Resistance Force Modulation Multiple Spectroscopy modes Lithography and Manipulation modes Compatible Cantilevers Nanosurf easyscan 2 Scripting Interface Cantilevers should have the following properties: Grooves compatible with the alignment chip used by Applied Nanostructures, BudgetSensors, NanoSensors, NanoWorld, Nascatec, and VISTAprobes A nominal length of 225 µm or more, and a width of 40 µm or more A coating on the backside of the cantilever that reflects red light FlexAFM Video Camera Applications Included control software Remote control by Automating measurement tasks, lithography, custom evaluation functions, using third party measurement equipment,... Windows Scripting Host: Visual Basic Script, Java Script COM compatible languages: LabView, MathLab, Visual Basic, Delphi, C++, Camera system Camera resolution Color video camera for top view. High-contrast camera for side view. 3.1 MP ( pixels) for top view Zoom range 4-Fold digital zoom in 3 steps (1/2/4 ) Focus Video display Motorized, user-controlled focus for each camera Simultaneous display of top and side view in software Nanosurf easyscan 2 Signal Module S Available output signals Full scale corresponds to Power supply output X-Axis, Y-Axis, Z-Axis, Approach, Tip Voltage, STM Current or AFM Deflection, Excitation, Amplitude, Phase ± 10 V, Excitation: ± 5 V GND, +15 V, 15 V Video output Direct USB 2.0 connection Nanosurf easyscan 2 Signal Module A All output signals of Signal Module S Additional signal modulation inputs / outputs Free connectors X-Axis, Y-Axis, Z-Axis, Tip Voltage, Excitation 2 x Aux (connection made on user request) Modulation range ± 10 V (excitation: ± 5 V) User input / output 2 16 bit ADC / DAC converters, ± 10 V Microscopy Made Easy Additional analog user outputs Synchronisation output 2 16 bit D/A converters, ± 10 V 1 TTL: start, end, point sync Nanosurf AG Grammetstrasse 14 CH-4410 Liestal / Switzerland Phone: Fax: Additional modes Almost unlimited Nanosurf, Inc Center Avenue, Suite 507 Fort Lee, NJ / USA Phone: Fax: Nanosurf and the Nanosurf Logo are trademarks of Nanosurf AG, registered and/or otherwise protected in various countries. Copyright 2009 Nanosurf AG, Switzerland BT Specifications are subject to change without notice.

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