OBIS CellX. The Universal Light Engine FEATURES

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1 OBIS CellX The Universal Light Engine OBIS CellX is a multi-wavelength platform for use as the laser excitation Light Engine in applications requiring or laser wavelengths from a single module. CellX delivers up to four wavelengths from a single, compact module that includes user-adjustable steering and telescopes used to optimize the beams to your target requirements. For example, CellX can be aligned to give flexible patterns of focused stripes in a flow cytometer. Using the same optical Cores that are at the heart of Coherent s OBIS suite of lasers, CellX delivers best-in-class optical performance and reliability. By consolidating control, thermal management and packaging, CellX reduces complexity encountered when integrating multiple separate lasers. The savings that come from deleting redundant elements is reinvested into features to benefit your application. FEATURES Up to wavelengths OBIS Performance Common power, control and I/O interfaces User-adjustable beam steering and telescopes APPLICATIONS Flow Cytometry Microscopy Medical Imaging Optogenetics CellX has a single electrical interface for ease of installation. Each laser wavelength can be individually controlled via RS- or USB computer interface, or via analog and digital control lines. With its low cost, alignment flexibility and ease-of-integration, CellX is the universal laser Light Engine for your application.

2 OPTICAL SPECIFICATIONS Wavelength (nm) Output Power (mw) Part Number n/a n/a Spatial Mode TEM 00 M (Beam Quality). Beam Asymmetry Beam Diameter at /e (mm) Beam Divergence (mrad, full angle) 0. Pointing Stability Over Temperature (µrad/ C) <0 :. Beam Colinearity (µrad) <00 RMS Noise 5 (%) (0 Hz to 0 MHz) <0.5 Peak-to-Peak Noise 5 (%) (0 Hz to 0 khz) < Long-term Power Stability (%) (8 hours, ± C) < Warm-up Time 6 (minutes) (from cold start) <5 Polarization Extinction Ratio >50: >75: >50: >50: Polarization Azimuth Vertical ±5 CONTROL SPECIFICATIONS Interface for Computer Control Laser Drive Modes (Four Operating Modes, individually selected for each wavelength thru USB or RS-) Digital Modulation USB (mini-b) and RS- (from DB7, 500 Baud) ) CW with Power Control via USB/RS- ) Analog Modulation ) Digital Modulation ) Mixed Analog and Digital Modulation (simultaneous Analog and Digital) Connection on DB7 Interface Pin Pin Pin Pin 7 Voltage and Impedance 0-.V 7, kohm input impedance each, Normally Low (off) Maximum Bandwidth (khz) 50 Rise Time (0% to 90%) (µsec) <5 Fall Time (90% to 0%) (µsec) <5 Modulation Depth (extinction ratio) Power Range Analog Modulation Infinite Modulate from 0% to Set Power (USB or RS-) in Digital Mode Connection on DB7 Interface Pin Pin Pin 6 Pin 6 Voltage and Impedance 0 to 5V, kohm input impedance each, Normally Low (off) Maximum Bandwidth, db (khz) 50 Rise Time (0% to 90%) (µsec) <5 Fall Time (90% to 0%) (µsec) <5 Modulation Depth (extinction ratio) >50: >50: >50: >50: Power Range Modulate from 0% to 0% with 0 to 5V in Analog Mode Laser-to-laser center wavelength tolerance: 05 nm ±5 nm. 88 nm and 56 nm with ± nm, 60 nm with 6 to 6 nm range. Short pass filter suppression of out-of-band emission for 60 nm. Output power is measured at the output window of CellX. Power is variable in CW Mode from 5% (05 nm and 60 nm) to 0% of rated power. Output power is variable in CW Mode from 0 mw (88 nm and 56 nm) to 0% of rated power. Specifications are valid for 00% power. For 88 nm and 56 nm any residual laser emission at 808 nm fundamental is <0. mw. Beam Quality (M ) measured per laser channel using ModeMaster with 90/0 clip levels. Standard alignment. User adjustable 5 RMS Noise and Peak-to-Peak Noise Specifications are per laser channel, during CW operation. 6 Typical power-on delay of minute from cold start. 7 Digital input is 5V tolerant.

3 MECHANICAL AND ENVIRONMENTAL SPECIFICATIONS MECHANICAL SPECIFICATIONS OBIS CellX Dimensions (mm) (L x D x H) 55 x 80 x 5. Beam Position from Reference (mm) <0.5 Beam Angle (mrad) <5 Laser Safety Classification ESD Protection Baseplate Operating Temperature ( C) 0 to 5 Heat Dissipation of Laser Head (Watts) Typical 0, Maximum 60 Ambient Temperature ( C) 0 to 5 Non-Operating Condition ( C) -0 to +60 Shock Tolerance (6 ms) Weight (kg). ELECTRICAL SPECIFICATIONS Power Input Connector Supply Voltage (V DC) EN66- (8 kv Air Discharge, kv Contact Discharge) 0g Use Molex for Power Cable Connector, Pins,, for Power, Pins,5,6 for Ground ± (00 Watt minimum) Power Consumption (W) Typical 0, Maximum 60 See mechanical drawing. OEM Product - does not comply with CDRH CFR 00.0 and 00. without appropriate integration. Typically 85% of heat load through the base plate. See Users Manual for more detail. Non-Condensing. See User Manual for more detail. OBIS CellX 0.0 mm (0.9 in.).0 mm (0.6 in.) 8.7 mm (7.7 in.) 80.0 mm (7.09 in.) 60.0 mm (6.0 in.) X Ø.8 mm (0.9 in.) Thru All Mounting Holes for M or #8 Socket Head Cap Screws 7.0 mm (5.79 in.) Top View 55.0 mm (6.0 in.) X screws to remove lid to access beam adjustments X -0 UNC.8 mm (0.9 in.) Tapped Holes for Optional Objective or Accessory Mounting Ø 5.6 mm (.0 in.) Front View Status Indicator USB Laser Control I/O DB7 Power Recepticle Interlock Indicator.8 mm (.77 in.) 5. mm (.06 in.) 5. mm (.00 in.).8 mm (.5 in.) Laser Aperture Ø 0. mm (0.0 in.) Rear View

4 OBIS CellX Objective Lens Accessories Datasheet 5 OPTICAL PERFORMANCE OBIS CellX Objective Lens Accessories SPECIFICATIONS OL0-VIS OL5-VIS Part Number Wavelength Range (nm) 00 to 700 Beam Profile at Focus (Vertical, Horizontal) Gaussian, Gaussian Focus Spot Size Vertical (µm) (/e ) 0 ± 5 ± Focus Side-lobes Vertical (% of peak) <0 Focus Spot Size Horizontal (µm) (/e ) Adjustable from 60 ±5 to 80 ±0 Adjustable from 90 ±0 to 0 ±0 Working Distance (mm) Dimensions (mm) x x 56 x x 7.7 Vertical Adjustment, (µm) ±50 Horizontal Adjustment, (µm) ±50 Focus Adjustment 5 Independent focus adjustment of all wavelengths Measured at location of best vertical focus. System aligned to lower limit on delivery. Horizontal beam size can be adjusted up to the upper limit. Pre-alignment to a wider horizontal waist is available. Measured from mechanical surface (output end) of the objective assembly - see drawing. Measured from nominal beam axis. Adjustment using tilt/yaw adjustment internal to CellX, while meeting all optical specifications. Assumes the objective assembly mounted within less than 00 mm (optical path length) from the output face of CellX. Adjustment using telescope adjustment internal to CellX, while meeting all optical specifications. NOMINAL OPTICAL PERFORMANCE Nominal optical performance through focus OL0-VIS Nominal optical performance through focus OL5-VIS Beam Diameter (µm) Working Distance (mm) 05 X 88 X 56 X 67 X 05 Y 88 Y 56 Y 67 Y Beam Diameter (µm) Working Distance (mm) 05 X 88 X 56 X 67 X 05 Y 88 Y 56 Y 67 Y Flow cytometry example: four laser focus with separated positions - user adjustable

5 PART NUMBER LASER 8680 CellX Laser x50 mw 05, 88, 67 nm 868 CellX Laser x50 mw 05, 88, 56, 67 nm 868 CellX Laser x00 mw 05, 88, 67 nm 868 CellX Laser x00 mw 05, 88, 56, 67 nm PART NUMBER ACCESSORY 5 CellX System x00 mw 05, 88, 56, 67 nm Developers Kit 0 Accessory Kit for CellX (Alignment Tools, Interlock Plug, USB Cable, Coherent Connection, User Manual) 985 Accessory, Objective Lens, OL0-VIS 0 µm Focus, CellX 986 Accessory, Objective Lens, OL0-VIS 5 µm Focus, CellX 96 Accessory, Mount, Front Aperture Objective Holder, CellX 96 Accessory, Translation Stage with Mount for Objective Lens, CellX 85 Heatsink, Fan-Cooled with Stage Platform Extension, CellX 5 Heatsink, OEM, CellX 999 Accessory, Control Board, Adjustable Power, CellX 9865 Accessory, Control Board, Key-Switch, RS-, Digital/Analog SMB, CellX 60 Accessory, Interlock Plug, DB7, CellX 597 Accessory, Control Board, Analog Modulation Inputs, RS- Coherent, Inc., 500 Patrick Henry Drive Santa Clara, CA 9505 p. (800) (08) f. (08) tech.sales@coherent.com Coherent follows a policy of continuous product improvement. Specifications are subject to change without notice. Coherent s scientific and industrial lasers are certified to comply with the Federal Regulations ( CFR Subchapter J) as administered by the Center for Devices and Radiological Health on all systems ordered for shipment after August, 976. Coherent offers a limited warranty for all OBIS CELLX lasers. For full details of this warranty coverage, please refer to the Service section at or contact your local Sales or Service Representative. Printed in the U.S.A. MC-xxx-7-0M07 Copyright 07 Coherent, Inc.

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