DNA Parentage Test No Summary Report

Transcription

1 Collaborative Testing Services, Inc FORENSIC TESTING PROGRAM DNA Parentage Test No Summary Report This proficiency test was sent to 45 participants. Each participant received a sample pack consisting of the standard paternity trio, collected from a mother, a son, and the potential father. Participants were requested to analyze the samples using their existing protocols. Data were returned from 42 participants (93% response rate) and are compiled into the following tables: Page Manufacturer's Information 2 Summary Comments 4 Table 1: STR Amplification Kit(s) & Results 5 Table 2: Item 3 Paternity Index Results 27 Table 3: YSTR Amplification Kit(s) & Results 34 Table 4: Additional DNA & PI Results 42 Table 5: Paternity DNA Statistics 43 Table : Paternity Conclusions 45 Table 7: Kinship DNA Statistics 4 Table 8: Additional Kinship Statistical Results 48 Table 9: Additional Comments 51 Appendix: Data Sheet 53 This report contains the data received from the participants in this test. Since these participants are located in many countries around the world, and it is their option how the samples are to be used (e.g., training exercise, known or blind proficiency testing, research and development of new techniques, etc.), the results compiled in the Summary Report are not intended to be an overview of the quality of work performed in the profession and cannot be interpreted as such. The Summary Comments are included for the benefit of participants to assist with maintaining or enhancing the quality of their results. These comments are not intended to reflect the general state of the art within the profession. Participant results are reported using a randomly assigned "WebCode". This code maintains participant's anonymity, provides linking of the various report sections, and will change with every report.

2 DNA Parentage Test Manufacturer's Information Each sample set was a collection of known blood samples, provided on FTA Micro cards, from three individuals (Items 13); a mother, a son, and a potential father. Participants were requested to analyze these items using their existing protocols. Also included in the data sheet was a kinship exercise that consisted of autosomal DNA profiles of two individuals for comparison. Participants were requested to determine if an half sibling relationship claim was supported following the review of these profiles. SAMPLE PREPARATION: All stains were prepared from human whole blood which was drawn into EDTA tubes. Item 1 (75 μl) was blood from a female (mother) donor, Item 2 (75 μl) was from a male (son) donor and Item 3 (75 μl) was created using blood collected from a male donor who was the biological father of the Item 2 male. The different Items were prepared at separate times and were packaged once they were thoroughly dried. Completed sample sets were stored at 20 C until shipment on May 15th, SAMPLE SET ASSEMBLY: For each sample set, all three Items (13) were placed in a prelabeled sample pack envelope and sealed. The sample pack envelopes were then packaged in prelabeled Heat Seal envelopes and sealed. This process was repeated until all of the sample sets were prepared. KINSHIP EXERCISE: This exercise included allelic results representing a half sibling relationship. VERIFICATION: Laboratories that conducted predistribution analysis of the samples reported consistent results and associations. ( 2 )

3 DNA Parentage Test Amelogenin and STR Results Results compiled from predistribution laboratories and a consensus of at least 10 participants. Item D2S441 D3S1358 D4S2408 D5S818 DS1043 D8S1179 D9S1122 D10S1248 D12S391 D1S539 D17S1301 D18S51 D19S433 D20S482 D21S11 D22S1045 Amelogenin CSF1PO 1 13, ,20 11,11.3 * * 11,14 * 14, ,25 9,9 * * 11,11 5,12 18, ,18 18, ,14 * * 9,14 * 17.3,22 12,12 * * 15,17 5, , ,24 * * 9,10 * 12,15 18,22 * * 9, ,31.2 Item 2 3 YSTR Results Results compiled from predistribution laboratories and a consensus of at least 10 participants. DYF387S1 DYS19 DYS385 DYS389I DYS389II DYS390 DYS391 DYS392 DYS393 DYS437 DYS438 DYS439 DYS448 DYS449 DYS45 DYS458 DYS40 DYS481 DYS505 DYS518 DYS522 DYS533 DYS549 DYS570 DYS57 DYS12 DYS27 DYS35 DYS43 YGATAH4 Y Indel * 14 11, * 1 1 * 21 * * * 12 * * * 24 * 12 * * 14 11, * 1 1 * 21 * * * 12 * * * 24 * 12 * Paternity Indices Median Paternity Index results compiled from predistribution laboratories and a consensus of at least 10 participants. Item D2S441 D3S1358 D4S2408 D5S818 Database DS1043 D8S1179 D9S1122 D10S1248 D12S391 D1S539 D17S1301 D18S51 D19S433 D20S482 D21S11 D22S1045 Amelogenin CSF1PO 3 FBI * * * PopStats * * * * * * 2.77 * * * * * NIST * STRBASE * * * * * * * Results were not received from a minimum of 10 participants for the loci indicated. Printed: August 31, 2017 ( 3 )

4 DNA Parentage Test Summary Comments The DNA Parentage test was designed to allow participants to assess their proficiency in the analysis and interpretation of a standard trio of blood samples on FTA Micro cards. Item 1 was blood collected from a female donor (mother), Item 2 was blood collected from a male donor (son), and Item 3 was blood collected from a male donor who is the biological father of the Item 2 male. Participants were requested to analyze the samples and provide allelic and statistical results as well as relationship conclusions. Sample sets also included a kinship exercise where participants were requested to evaluate the provided DNA profiles and report the kinship index and relationship conclusions (Refer to the Manufacturer's Information for preparation details). DNA Analysis: All 42 participants who returned data reported results for STRs for all three items. The individual profiles for Item 1 and Item 3 were concordant across all participants. Item 2 had two participants with discordant results. One participant reported an allele call of at Amelogenin where the consensus was. Another participant reported a 10,3 at the D8S1179 locus where the consensus was. Lastly, all 31 participants returned concordant data for YSTR analysis for Item 2 and Item 3. Paternity DNA Statistics: Of the 42 participants who returned data, 41 provided a combined Paternity Index calculation and reported a specific population database. The FBI PopStats database and the NISTSTRBASE were reported most frequently by participants. The averaged combined Paternity Index for those participants using the FBI PopStats database is 5.7E+10 while the averaged combined Paternity Index for those participants using the NISTSTRBASE database is 3.24E+12. All the 41 participants that provided combined Paternity Index calculations generated a probability of paternity greater than or equal to 99.9%. For the paternity conclusions, all 42 participants who returned data responded that the male donor of Item 3 could not be excluded as the father of the son (Item 2). Kinship DNA Statistics: There were 22 participants who responded for the kinship exercise. Of the participants who responded, 19 reported that the Caucasian Half Sibling relationship was supported by the genetic evidence. One participant reported that there was not enough genetic evidence to make a conclusion, however the half sibling relationship could not be excluded. One participant reported that the DNA evidence did not support the proposed relationship. Another participant reported that a full sibling relationship was supported instead of half siblings ( 4 )

5 DNA Parentage Test STR Amplification Kit(s) & Results Item Amplification Kits DS1043 D21S11 D1S539 D22S1045 TABLE 1 D2S441 D3S1358 D4S2408 D5S818 D8S1179 D9S1122 D10S1248 D12S391 D17S1301 D18S51 D19S433 D20S482 Amelogenin CSF1PO Item 1 STR Results 22HHME PowerPlex c 13, ,20 11, ,25 9 X 11 5,12 18,29.2 2BPGWF PowerPlex Fusion 13, ,20 11, ,25 9 X 11 5,12 3Y9UME PowerPlex Fusion 13, ,20 11, ,25 9 X 11 5,12 4WJ8B Identifiler PLUS 18,20 9,9 11,11 J8Y3J VeriFiler Express 13, ,20 11, ,19 11,14 14, ,25 9,9 11,11 5,12 ( 5 )

6 DNA Parentage Test TABLE 1 Item Amplification Kits DS1043 D21S11 D1S539 D22S1045 D2S441 D3S1358 D4S2408 D5S818 D8S1179 D9S1122 D10S1248 D12S391 D17S1301 D18S51 D19S433 D20S482 Amelogenin CSF1PO Item 1 STR Results 7TNE GlobalFiler 13, ,20 11, , ,25 9,9 11,11 18,29.2 7L7RH GlobalFiler 13, ,20 11, ,25 9 X 11 18,29.2 7EAZ4E GlobalFiler 13, ,20 11, , ,25 9,9 11,11 18,29.2 7FFFB PowerPlex Fusion 13, ,20 11, ,25 9 X 11 5,12 7ZA2FH Identifiler Direct 18, FHCKF Investigator 24plex 13, ,20 11, , ,25 9,9 11,11 18,29.2 ( )

7 DNA Parentage Test TABLE 1 Item Amplification Kits DS1043 D21S11 D1S539 D22S1045 D2S441 D3S1358 D4S2408 D5S818 D8S1179 D9S1122 D10S1248 D12S391 D17S1301 D18S51 D19S433 D20S482 Amelogenin CSF1PO Item 1 STR Results 9DPXAB PowerPlex 21 13, , ,19 11, ,25 9,9 11,11 5,12 9VQKZA PowerPlex Fusion 13, ,20 11, ,25 9 X 11 5,12 A2B3UC PowerPlex 21 13, , ,19 11, ,25 9,9 11,11 5,12 AAKUWE GlobalFiler 13, ,20 11, , ,25 9,9 11,11 18,29.2 CRZKB Identifiler Direct 18, , D3UFL PowerPlex Fusion 13, ,20 11, ,25 9 X 11 5,12 ( 7 )

8 DNA Parentage Test TABLE 1 Item Amplification Kits DS1043 D21S11 D1S539 D22S1045 D2S441 D3S1358 D4S2408 D5S818 D8S1179 D9S1122 D10S1248 D12S391 D17S1301 D18S51 D19S433 D20S482 Amelogenin CSF1PO Item 1 STR Results DFJFC8 PowerPlex 21 13, , ,19 11, ,25 9,9 11,11 5,12 DGEVP4 PowerPlex FUSION SYSTEM 13, ,20 11, , ,25 9,9 11,11 5,12 EGQW9H Identifiler Direct 18,20 9,9 11,11 ERQMR4 PowerPlex PP21 13, , ,19 11, ,25 9 X 11 5,12 FTD8G PowerPlex FUSION, GlobalFiler EXPRESS 13, ,20 11, , ,25 9,9 11,11 5,12 18,29.2 G9CPNY Identifiler Plus 18,20 9,9 11,11 ( 8 )

9 DNA Parentage Test TABLE 1 Item Amplification Kits DS1043 D21S11 D1S539 D22S1045 D2S441 D3S1358 D4S2408 D5S818 D8S1179 D9S1122 D10S1248 D12S391 D17S1301 D18S51 D19S433 D20S482 Amelogenin CSF1PO Item 1 STR Results JNGXGX Identifiler Direct 18, , K4MKGY Investigator IDPlex Plus 18,20 9,9 11,11 KNB49B Identifiler Plus,, 18,20 9 X 11 5,12 LYQCCV GlobalFiler 18,20 9 X 11 N7ZHZX PowerPlex 21 13, , ,19 11, ,25 9,9 11,11 5,12 NVZV92 GlobalFiler 13, ,20 11, , ,25 9,9 11,11 18,29.2 ( 9 )

10 DNA Parentage Test TABLE 1 Item Amplification Kits DS1043 D21S11 D1S539 D22S1045 D2S441 D3S1358 D4S2408 D5S818 D8S1179 D9S1122 D10S1248 D12S391 D17S1301 D18S51 D19S433 D20S482 Amelogenin CSF1PO Item 1 STR Results QHP2UT Identifiler Plus 18,20 9,9 11,11 R7QKHX GlobalFiler 13, ,20 11, , ,25 9,9 11,11 18,29.2 RCVLHR PowerPlex FUSION 5C 13, ,20 11, , ,25 9,9 11,11 5,12 T4TUM3 Identifiler Plus 18,20 9,9 11,11 VGU8YK PowerPlex fusion, powerplex esx 17 13, ,20 11, , ,25 9,9 11,11 5,12 18,29.2 WNA8TM PowerPlex Fusion C 13, ,20 11, , ,12 18,29.2 ( 10 )

11 DNA Parentage Test TABLE 1 Item Amplification Kits DS1043 D21S11 D1S539 D22S1045 D2S441 D3S1358 D4S2408 D5S818 D8S1179 D9S1122 D10S1248 D12S391 D17S1301 D18S51 D19S433 D20S482 Amelogenin CSF1PO Item 1 STR Results WNABJR GlobalFiler Express 13, ,20 11, ,25 9 X 11 18,29.2 Y72X3L PowerPlex 21 13, , ,19 11, ,25 9,9 11,11 5,12 YDNNNG 18,20 9,9 11,11 YYKJYG Identifiler Plus 18,20 9,9 11,11 Z9L4YN PowerPlex Fusion 5C 13, ,20 11, , ,25 9,9 11,11 5,12 ZDF4DJ PowerPlex Fusion, NGM SE 13, ,20 11, , ,25 9,9 11,11 5,12 18,29.2 ( 11 )

12 DNA Parentage Test TABLE 1 Item Amplification Kits DS1043 D21S11 D1S539 D22S1045 D2S441 D3S1358 D4S2408 D5S818 D8S1179 D9S1122 D10S1248 D12S391 D17S1301 D18S51 D19S433 D20S482 Amelogenin CSF1PO Item 1 STR Results ZHCMFG Identifiler Direct 18, , ( 12 )

13 DNA Parentage Test TABLE 1 Item Amplification Kits DS1043 D21S11 D1S539 D22S1045 D2S441 D3S1358 D4S2408 D5S818 D8S1179 D9S1122 D10S1248 D12S391 D17S1301 D18S51 D19S433 D20S482 Amelogenin CSF1PO Item 2 STR Results 22HHME PowerPlex c 17.3,18 18, , , ,17 5, BPGWF PowerPlex Fusion 17.3,18 18, , , ,17 5,10 3Y9UME PowerPlex Fusion 17.3,18 18, , , ,17 5,10 4WJ8B Identifiler PLUS 18,19 12,12 J8Y3J VeriFiler Express 17.3,18 18, , ,19 9, ,22 12,12 15,17 5,10 7TNE GlobalFiler 17.3,18 18, , ,22 12,12 15, ,29.2 ( 13 )

14 DNA Parentage Test TABLE 1 Item Amplification Kits DS1043 D21S11 D1S539 D22S1045 D2S441 D3S1358 D4S2408 D5S818 D8S1179 D9S1122 D10S1248 D12S391 D17S1301 D18S51 D19S433 D20S482 Amelogenin CSF1PO Item 2 STR Results 7L7RH GlobalFiler 17.3,18 18, , , , EAZ4E GlobalFiler 17.3,18 18, , ,22 12,12 15, ,29.2 7FFFB PowerPlex Fusion 17.3,18 18, , , ,17 5,10 7ZA2FH Identifiler Direct 18, FHCKF Investigator 24plex 17.3,18 18, , ,22 12,12 15, ,29.2 9DPXAB PowerPlex ,18 18, ,19 9, ,22 12,12 5,10 ( 14 )

15 DNA Parentage Test TABLE 1 Item Amplification Kits DS1043 D21S11 D1S539 D22S1045 D2S441 D3S1358 D4S2408 D5S818 D8S1179 D9S1122 D10S1248 D12S391 D17S1301 D18S51 D19S433 D20S482 Amelogenin CSF1PO Item 2 STR Results 9VQKZA PowerPlex Fusion 17.3,18 18, , , ,17 5,10 A2B3UC PowerPlex ,18 18, ,19 9, ,22 12,12 5,10 AAKUWE GlobalFiler 17.3,18 18, , ,22 12,12 15, ,29.2 CRZKB Identifiler Direct 18,19 2 9,14 12 D3UFL PowerPlex Fusion 17.3,18 18, , , ,17 5,10 DFJFC8 PowerPlex ,18 18, ,19 9, ,22 12,12 5,10 ( 15 )

16 DNA Parentage Test TABLE 1 Item Amplification Kits DS1043 D21S11 D1S539 D22S1045 D2S441 D3S1358 D4S2408 D5S818 D8S1179 D9S1122 D10S1248 D12S391 D17S1301 D18S51 D19S433 D20S482 Amelogenin CSF1PO Item 2 STR Results DGEVP4 PowerPlex Fusion System 17.3,18 18, ,14 10,3 17.3,22 12,12 15,17 5,10 EGQW9H Identifiler Direct 18,19 12,12 ERQMR4 PowerPlex PP ,18 18, ,19 9, , ,10 FTD8G PowerPlex FUSION, GlobalFiler EXPRESS 17.3,18 18, , ,22 12,12 15,17 5, ,29.2 G9CPNY Identifiler plus 18,19 12,12 JNGXGX Identifiler Direct 18,19 2 9,14 12 ( 1 )

17 DNA Parentage Test TABLE 1 Item Amplification Kits DS1043 D21S11 D1S539 D22S1045 D2S441 D3S1358 D4S2408 D5S818 D8S1179 D9S1122 D10S1248 D12S391 D17S1301 D18S51 D19S433 D20S482 Amelogenin CSF1PO Item 2 STR Results K4MKGY IDplex Plus 18,19 12,12 KNB49B Identifiler Plus, and 18, ,10 LYQCCV GlobalFiler 18,19 12 N7ZHZX PowerPlex ,18 18, ,19 9, ,22 12,12 5,10 NVZV92 GlobalFiler 17.3,18 18, , ,22 12,12 15, ,29.2 QHP2UT Identifiler Plus 18,19 12,12 ( 17 )

18 DNA Parentage Test TABLE 1 Item Amplification Kits DS1043 D21S11 D1S539 D22S1045 D2S441 D3S1358 D4S2408 D5S818 D8S1179 D9S1122 D10S1248 D12S391 D17S1301 D18S51 D19S433 D20S482 Amelogenin CSF1PO Item 2 STR Results R7QKHX GlobalFiler 17.3,18 18, , ,22 12,12 15, ,29.2 RCVLHR PowerPlex FUSION 5C 17.3,18 18, , ,22 12,12 15,17 5,10 T4TUM3 Identifiler Plus 18,19 12,12 VGU8YK PowerPlex FUSION, PowerPlex ESX ,18 18, , ,22 12,12 15,17 5, ,29.2 WNA8TM PowerPlex Fusion C 17.3,18 18, , , ,17 5, WNABJR GlobalFiler Express 17.3,18 18, , , , ( 18 )

19 DNA Parentage Test TABLE 1 Item Amplification Kits DS1043 D21S11 D1S539 D22S1045 D2S441 D3S1358 D4S2408 D5S818 D8S1179 D9S1122 D10S1248 D12S391 D17S1301 D18S51 D19S433 D20S482 Amelogenin CSF1PO Item 2 STR Results Y72X3L PowerPlex ,18 18, ,19 9, ,22 12,12 5,10 YDNNNG 18,19 12,12 YYKJYG Identifiler Plus 18,19 12,12 Z9L4YN PowerPlex Fusion 5C 17.3,18 18, , ,22 12,12 15,17 5,10 ZDF4DJ PowerPlex Fusion, NGM SE 17.3,18 18, , ,22 12,12 15,17 5, ,29.2 ZHCMFG Identifiler Direct 18,19 12 ( 19 )

20 DNA Parentage Test TABLE 1 Item Amplification Kits DS1043 D21S11 D1S539 D22S1045 D2S441 D3S1358 D4S2408 D5S818 D8S1179 D9S1122 D10S1248 D12S391 D17S1301 D18S51 D19S433 D20S482 Amelogenin CSF1PO Item 3 STR Results 22HHME PowerPlex c 19, ,15 18, , ,31.2 2BPGWF PowerPlex Fusion 19, ,15 18, ,12 3Y9UME PowerPlex Fusion 19, ,15 18, ,12 4WJ8B Identifiler PLUS 19,24 J8Y3J VeriFiler Express 19,24 3 9,10 12,15 18,22 9,12 7TNE GlobalFiler 19,24 12,15 18, ,31.2 ( 20 )

21 DNA Parentage Test TABLE 1 Item Amplification Kits DS1043 D21S11 D1S539 D22S1045 D2S441 D3S1358 D4S2408 D5S818 D8S1179 D9S1122 D10S1248 D12S391 D17S1301 D18S51 D19S433 D20S482 Amelogenin CSF1PO Item 3 STR Results 7L7RH GlobalFiler 19, ,15 18, ,31.2 7EAZ4E GlobalFiler 19,24 12,15 18, ,31.2 7FFFB PowerPlex Fusion 19, ,15 18, ,12 7ZA2FH Identifiler Direct 19, FHCKF Investigator 24plex 19,24 12,15 18, ,31.2 9DPXAB PowerPlex 21 19,24 3 9,10 18,22 9,12 ( 21 )

22 DNA Parentage Test TABLE 1 Item Amplification Kits DS1043 D21S11 D1S539 D22S1045 D2S441 D3S1358 D4S2408 D5S818 D8S1179 D9S1122 D10S1248 D12S391 D17S1301 D18S51 D19S433 D20S482 Amelogenin CSF1PO Item 3 STR Results 9VQKZA PowerPlex Fusion 19, ,15 18, ,12 A2B3UC PowerPlex 21 19,24 3 9,10 18,22 9,12 AAKUWE GlobalFiler 19,24 12,15 18, ,31.2 CRZKB Identifiler Direct 19,24 3 9, D3UFL PowerPlex Fusion 19, ,15 18, ,12 DFJFC8 PowerPlex 21 19,24 3 9,10 18,22 9,12 ( 22 )

23 DNA Parentage Test TABLE 1 Item Amplification Kits DS1043 D21S11 D1S539 D22S1045 D2S441 D3S1358 D4S2408 D5S818 D8S1179 D9S1122 D10S1248 D12S391 D17S1301 D18S51 D19S433 D20S482 Amelogenin CSF1PO Item 3 STR Results DGEVP4 PowerPlex Fusion System 19,24 12,15 18,22 9,12 EGQW9H Identifiler Direct 19,24 ERQMR4 PowerPlex PP21 19,24 3 9, , ,12 FTD8G PowerPlex FUSION1, GlobalFiler EXPRESS 19,24 12,15 18,22 9, ,31.2 G9CPNY Identifiler Plus 19,24 JNGXGX Identifiler Direct 19,24 3 9, ( 23 )

24 DNA Parentage Test TABLE 1 Item Amplification Kits DS1043 D21S11 D1S539 D22S1045 D2S441 D3S1358 D4S2408 D5S818 D8S1179 D9S1122 D10S1248 D12S391 D17S1301 D18S51 D19S433 D20S482 Amelogenin CSF1PO Item 3 STR Results K4MKGY IDplex Plus 19,24 KNB49B Identifiler Plus 19, ,12 LYQCCV GlobalFiler 19, N7ZHZX PowerPlex 21 19,24 3 9,10 18,22 9,12 NVZV92 GlobalFiler 19,24 12,15 18, ,31.2 QHP2UT Identifiler Plus 19,24 ( 24 )

25 DNA Parentage Test TABLE 1 Item Amplification Kits DS1043 D21S11 D1S539 D22S1045 D2S441 D3S1358 D4S2408 D5S818 D8S1179 D9S1122 D10S1248 D12S391 D17S1301 D18S51 D19S433 D20S482 Amelogenin CSF1PO Item 3 STR Results R7QKHX GlobalFiler 19,24 12,15 18, ,31.2 RCVLHR PowerPlex FUSION 5C 19,24 12,15 18,22 9,12 T4TUM3 Identifiler Plus 19,24 VGU8YK PowerPlex FUSION, PowerPlex ESX17 19,24 12,15 18,22 9, ,31.2 WNA8TM PowerPlex Fusion C 19, ,15 18, , ,31.2 WNABJR GlobalFiler 19, ,15 18, ,31.2 ( 25 )

26 DNA Parentage Test TABLE 1 Item Amplification Kits DS1043 D21S11 D1S539 D22S1045 D2S441 D3S1358 D4S2408 D5S818 D8S1179 D9S1122 D10S1248 D12S391 D17S1301 D18S51 D19S433 D20S482 Amelogenin CSF1PO Item 3 STR Results Y72X3L PowerPlex 21 19,24 3 9,10 18,22 9,12 YDNNNG 19,24 YYKJYG Identifiler Plus 19,24 Z9L4YN PowerPlex Fusion 5C 19,24 12,15 18,22 9,12 ZDF4DJ PowerPlex Fusion, NGM SE 19,24 12,15 18,22 9, ,31.2 ZHCMFG Identifiler Direct 19,24 3 9, ( 2 )

27 DNA Parentage Test Item 3 Paternity Index Results TABLE 2 Item Population Database(s) DS1043 D21S11 D1S539 D22S1045 D2S441 D3S1358 D4S2408 D5S818 D8S1179 D9S1122 D10S1248 D12S391 D17S1301 D18S51 D19S433 D20S482 Amelogenin CSF1PO Item 3 Paternity Index Results 22HHME NISTSTRBASE BPGWF NISTSTRBASE Y9UME FBI PopStats WJ8B NISTSTRBASE J8Y3J Laboratory database TNE NISTSTRBASE ( 27 )

28 DNA Parentage Test Item Population Database(s) DS1043 D21S11 D1S539 D22S1045 TABLE 2 D2S441 D3S1358 D4S2408 D5S818 D8S1179 D9S1122 D10S1248 D12S391 D17S1301 D18S51 D19S433 D20S482 Amelogenin CSF1PO Item 3 Paternity Index Results 7L7RH FBI PopStats EAZ4E [Country] Caucasian Pop. Database ([Organization]) FFFB NISTSTRBASE ZA2FH NISTSTRBASE FHCKF NISTSTRBASE DPXAB Powerplex 21 [Country] ( 28 )

29 DNA Parentage Test Item Population Database(s) DS1043 D21S11 D1S539 D22S1045 TABLE 2 D2S441 D3S1358 D4S2408 D5S818 D8S1179 D9S1122 D10S1248 D12S391 D17S1301 D18S51 D19S433 D20S482 Amelogenin CSF1PO Item 3 Paternity Index Results 9VQKZA NISTSTRBASE A2B3UC Laboratory specific database AAKUWE FBI PopStats CRZKB NISTSTRBASE D3UFL NISTSTRBASE DFJFC8 NISTSTRBASE ( 29 )

30 DNA Parentage Test Item Population Database(s) DS1043 D21S11 D1S539 D22S1045 TABLE 2 D2S441 D3S1358 D4S2408 D5S818 D8S1179 D9S1122 D10S1248 D12S391 D17S1301 D18S51 D19S433 D20S482 Amelogenin CSF1PO Item 3 Paternity Index Results DGEVP4 [Country] Caucasian EGQW9H NISTSTRBASE ERQMR4 Promega FTD8G NISTSTRBASE G9CPNY FBI PopStats JNGXGX NISTSTRBASE ( 30 )

31 DNA Parentage Test Item Population Database(s) DS1043 D21S11 D1S539 D22S1045 TABLE 2 D2S441 D3S1358 D4S2408 D5S818 D8S1179 D9S1122 D10S1248 D12S391 D17S1301 D18S51 D19S433 D20S482 Amelogenin CSF1PO Item 3 Paternity Index Results K4MKGY NISTSTRBASE KNB49B FBI PopStats, Promega database for or LYQCCV Life Technologies Database N7ZHZX Powerplex 21 [Country] Caucasian Dataset NVZV92 FBI PopStats QHP2UT NISTSTRBASE ( 31 )

32 DNA Parentage Test Item R7QKHX TABLE 2 Population Database(s) D2S441 D3S1358 D4S2408 D5S818 DS1043 D8S1179 D9S1122 D10S1248 D12S391 D1S539 D17S1301 D18S51 D19S433 D20S482 D21S11 D22S1045 Amelogenin CSF1PO Item 3 Paternity Index Results FBI PopStats N/A RCVLHR FBI PopStats T4TUM3 Local/State Database VGU8YK NISTSTRBASE WNA8TM NISTSTRBASE WNABJR FBI PopStats ( 32 )

33 DNA Parentage Test Item Population Database(s) DS1043 D21S11 D1S539 D22S1045 TABLE 2 D2S441 D3S1358 D4S2408 D5S818 D8S1179 D9S1122 D10S1248 D12S391 D17S1301 D18S51 D19S433 D20S482 Amelogenin CSF1PO Item 3 Paternity Index Results Y72X3L NISTSTRBASE YYKJYG NISTSTRBASE Z9L4YN FBI PopStats ZDF4DJ [Country] Population ZHCMFG NISTSTRBASE ( 33 )

34 DNA Parentage Test YSTR Amplification Kit(s) & Results TABLE 3 Amplification Kit Item DYF387S1 DYS19 DYS385 DYS389_I DYS389_II DYS390 DYS391 DYS392 DYS393 DYS437 DYS438 DYS439 DYS448 DYS449 DYS45 DYS458 DYS40 DYS481 DYS505 DYS518 DYS522 DYS533 DYS549 DYS570 DYS57 DYS12 DYS27 DYS35 DYS43 YGATAH4 Y Indel Item 2 YSTR Results 22HHME PowerPlex Fusion c BPGWF Yfiler , Y9UME PowerPlex Fusion WJ8B Yfiler , TNE Yfiler Plus, GlobalFiler 2 35, , L7RH Yfiler Plus, GlobalFiler 2 35, , EAZ4E GlobalFiler FFFB Yfiler , ZA2FH Yfiler , FHCKF Investigator 24plex 2 11 ( 34 )

35 DNA Parentage Test TABLE 3 Amplification Kit Item DYF387S1 DYS19 DYS385 DYS389_I DYS389_II DYS390 DYS391 DYS392 DYS393 DYS437 DYS438 DYS439 DYS448 DYS449 DYS45 DYS458 DYS40 DYS481 DYS505 DYS518 DYS522 DYS533 DYS549 DYS570 DYS57 DYS12 DYS27 DYS35 DYS43 YGATAH4 Y Indel Item 2 YSTR Results 9VQKZA Yfiler , AAKUWE Yfiler Plus 2 35, , CRZKB Yfiler , D3UFL Yfiler , DGEVP4 PowerPlex Fusion ERQMR4 PowerPlex Y Y , FTD8G PowerPlex Y 23, GlobalFiler , G9CPNY Yfiler plus 2 35, , JNGXGX Yfiler , K4MKGY Yfiler Plus 2 35, , ( 35 )

36 DNA Parentage Test TABLE 3 Amplification Kit Item DYF387S1 DYS19 DYS385 DYS389_I DYS389_II DYS390 DYS391 DYS392 DYS393 DYS437 DYS438 DYS439 DYS448 DYS449 DYS45 DYS458 DYS40 DYS481 DYS505 DYS518 DYS522 DYS533 DYS549 DYS570 DYS57 DYS12 DYS27 DYS35 DYS43 YGATAH4 Y Indel Item 2 YSTR Results NVZV92 Yfiler Plus 2 35, , QHP2UT Yfiler , R7QKHX Yfiler Plus 2 35, , RCVLHR PowerPlex Y , VGU8YK PowerPlex Y 23, PowerPlex Fusion , WNA8TM PowerPlex Fusion C WNABJR GlobalFiler YYKJYG Yfiler , Z9L4YN Yfiler , ZDF4DJ Yfiler , ( 3 )

37 DNA Parentage Test TABLE 3 Amplification Kit Item DYF387S1 DYS19 DYS385 DYS389_I DYS389_II DYS390 DYS391 DYS392 DYS393 DYS437 DYS438 DYS439 DYS448 DYS449 DYS45 DYS458 DYS40 DYS481 DYS505 DYS518 DYS522 DYS533 DYS549 DYS570 DYS57 DYS12 DYS27 DYS35 DYS43 YGATAH4 Y Indel Item 2 YSTR Results ZHCMFG Yfiler , ( 37 )

38 DNA Parentage Test TABLE 3 Amplification Kit Item DYF387S1 DYS19 DYS385 DYS389_I DYS389_II DYS390 DYS391 DYS392 DYS393 DYS437 DYS438 DYS439 DYS448 DYS449 DYS45 DYS458 DYS40 DYS481 DYS505 DYS518 DYS522 DYS533 DYS549 DYS570 DYS57 DYS12 DYS27 DYS35 DYS43 YGATAH4 Y Indel Item 3 YSTR Results 22HHME PowerPlex Fusion c BPGWF Yfiler , Y9UME PowerPlex Fusion WJ8B Yfiler , TNE Yfiler Plus, GlobalFiler 3 35, , L7RH Yfiler Plus, GlobalFiler 3 35, , EAZ4E GlobalFiler FFFB Yfiler , ZA2FH Yfiler , FHCKF Investigator 24plex 3 11 ( 38 )

39 DNA Parentage Test TABLE 3 Amplification Kit Item DYF387S1 DYS19 DYS385 DYS389_I DYS389_II DYS390 DYS391 DYS392 DYS393 DYS437 DYS438 DYS439 DYS448 DYS449 DYS45 DYS458 DYS40 DYS481 DYS505 DYS518 DYS522 DYS533 DYS549 DYS570 DYS57 DYS12 DYS27 DYS35 DYS43 YGATAH4 Y Indel Item 3 YSTR Results 9VQKZA Yfiler , AAKUWE Yfiler Plus 3 35, , CRZKB Yfiler , D3UFL Yfiler , DGEVP4 PowerPlex Fusion ERQMR4 PowerPlex Y Y , FTD8G PowerPlex Y 23, GlobalFiler , G9CPNY Yfiler Plus 3 35, , JNGXGX Yfiler , K4MKGY Yfiler Plus 3 35, , ( 39 )

40 DNA Parentage Test TABLE 3 Amplification Kit Item DYF387S1 DYS19 DYS385 DYS389_I DYS389_II DYS390 DYS391 DYS392 DYS393 DYS437 DYS438 DYS439 DYS448 DYS449 DYS45 DYS458 DYS40 DYS481 DYS505 DYS518 DYS522 DYS533 DYS549 DYS570 DYS57 DYS12 DYS27 DYS35 DYS43 YGATAH4 Y Indel Item 3 YSTR Results NVZV92 Yfiler Plus 3 35, , QHP2UT Yfiler , R7QKHX Yfiler Plus 3 35, , RCVLHR PowerPlex Y , VGU8YK PowerPlex Y 23, PowerPlex Fusion , WNA8TM PowerPlex Fusion C WNABJR GlobalFiler YYKJYG Yfiler , Z9L4YN Yfiler , ZDF4DJ Yfiler , ( 40 )

41 DNA Parentage Test TABLE 3 Amplification Kit Item DYF387S1 DYS19 DYS385 DYS389_I DYS389_II DYS390 DYS391 DYS392 DYS393 DYS437 DYS438 DYS439 DYS448 DYS449 DYS45 DYS458 DYS40 DYS481 DYS505 DYS518 DYS522 DYS533 DYS549 DYS570 DYS57 DYS12 DYS27 DYS35 DYS43 YGATAH4 Y Indel Item 3 YSTR Results ZHCMFG Yfiler Classic , ( 41 )

42 DNA Parentage Test Additional DNA & PI Results TABLE 4 Locus Item 1 Item 2 Item 3 DX10103 ZDF4DJ 18, DXS10074 ZDF4DJ 17, DXS10079 ZDF4DJ 1, DXS10101 ZDF4DJ 29.2, DXS10134 ZDF4DJ 3, DXS10135 ZDF4DJ 19, DXS1014 ZDF4DJ 25, DXS10148 ZDF4DJ 24.1, DXS7132 ZDF4DJ 13, DXS7423 ZDF4DJ 14, DXS8378 ZDF4DJ 11, Item 3 Paternity Index F13A ERQMR4 3.2, 3.2, F13B ERQMR4 9, FESFPS ERQMR HPRTB ZDF4DJ 12, LPL ERQMR4 11, PENTA C ERQMR4 11,13 5, ( 42 )

43 DNA Parentage Test Paternity DNA Statistics 22HHME Combined Paternity Index TABLE 5 Probability of Paternity 1.9E +12 N/A Population Database Used NISTSTRBASE 2BPGWF 3Y9UME 4WJ8B J8Y3J 7TNE 7L7RH 7EAZ4E 7FFFB 7ZA2FH 8FHCKF 9DPXAB 9VQKZA A2B3UC AAKUWE CRZKB D3UFL DFJFC8 DGEVP4 EGQW9H ERQMR4 FTD8G G9CPNY JNGXGX K4MKGY KNB49B 2.0 x 10^ % 99,380,000, % % 18,341,90, ,90,000, ,835,535,25 greater than 99.99% 20 billion % Billion >99.99% 219,320, % 20 billion 99.9% 1.904E ,90,000, % 2.0E E % 2,5E+13 99, N/A 1,24,915,02, ,150, % % ,300, % NISTSTRBASE FBI PopStats NISTSTRBASE Laboratory database NISTSTRBASE FBI PopStats [Country] Caucasian Pop. Database ([Organization]) NISTSTRBASE NISTSTRBASE NISTSTRBASE Powerplex 21 [Country] NISTSTRBASE Laboratory specific database FBI PopStats NISTSTRBASE NISTSTRBASE NISTSTRBASE [Country] Caucasian NISTSTRBASE Promega NISTSTRBASE FBI PopStats NISTSTRBASE NISTSTRBASE FBI PopStats, Promega database for or ( 43 )

44 DNA Parentage Test LYQCCV Combined Paternity Index TABLE 5 Probability of Paternity 1,243, Population Database Used Life Technologies Database N7ZHZX NVZV92 QHP2UT R7QKHX RCVLHR T4TUM3 VGU8YK WNA8TM WNABJR Y72X3L YYKJYG Z9L4YN ZDF4DJ ZHCMFG 7.2 x 10^8 N/A 42,90,000, , ,90,000, % % 1.8 E+12 > , e , % 40,000,000, e % % 99,380,000, , , % Powerplex 21 [Country] Caucasian Dataset FBI PopStats NISTSTRBASE FBI PopStats FBI PopStats Local/State Database NISTSTRBASE NISTSTRBASE FBI PopStats NISTSTRBASE NISTSTRBASE FBI PopStats [Country] Population NISTSTRBASE ( 44 )

45 DNA Parentage Test Paternity Conclusions TABLE Conclusions Conclusions 22HHME KNB49B 2BPGWF LYQCCV 3Y9UME N7ZHZX 4WJ8B NVZV92 J8Y3J QHP2UT 7TNE R7QKHX 7L7RH RCVLHR 7EAZ4E T4TUM3 7FFFB VGU8YK 7ZA2FH WNA8TM 8FHCKF WNABJR 9DPXAB Y72X3L 9VQKZA YDNNNG A2B3UC YYKJYG AAKUWE Z9L4YN CRZKB ZDF4DJ D3UFL ZHCMFG DFJFC8 DGEVP4 Response Summary Total: 42 EGQW9H ERQMR4 FTD8G Responses 42 Excluded 0 Inconclusive 0 G9CPNY JNGXGX K4MKGY ( 45 )

46 DNA Parentage Test Kinship DNA Statistics Is the claim of a Caucasian Half Siblings relationship supported by the genetic evidence? TABLE 7 Database Kinship Index Claim Supported? J8Y3J 7TNE 7ZA2FH AAKUWE CRZKB Laboratory database NISTSTRBASE NISTSTRBASE FBI PopStats NISTSTRBASE 2704 Yes 9300 Yes yes 2,412 Yes yes DGEVP4 [Country] Caucasian Full Siblings: LR=7,5E+4; Half Sibling: LR=1,E+4; Unrelated: Posterior probabilty= 0,00101 Genetic evidence suports the hypothesis that they are rather full siblings of Caucasian origin: LR=7,5E+4, LR for half siblings of Caucasian origin amounts 1,1E+4. EGQW9H ERQMR4 G9CPNY JNGXGX K4MKGY KNB49B N7ZHZX NVZV92 QHP2UT R7QKHX RCVLHR T4TUM3 NISTSTRBASE Promega FBI PopStats NISTSTRBASE NISTSTRBASE FBI PopStats, Promega database for or Powerplex 21 [Country] Caucasian Dataset FBI PopStats NISTSTRBASE FBI PopStats FBI PopStats Local/State Database 9300 Yes Yes 5,347,99 Yes yes 9, Yes, it is Yes 450 Yes 2,412 yes 9, Supported 2,412 Yes 5481 No, because there is not genetic evidence from the parents and/or there is a lack of genetic information, however they can not be excluded from being half siblings No, the DNA evidence does not support the relationship ( 4 )

47 DNA Parentage Test TABLE 7 Database Kinship Index Claim Supported? VGU8YK WNA8TM YYKJYG ZDF4DJ NISTSTRBASE NISTSTRBASE NISTSTRBASE [Country] Population 9318 Yes, it is 704 Yes, it is Yes YES ( 47 )

48 DNA Parentage Test Additional Kinship Statistical Results J8Y3J TABLE 8 Additional Statistical Results Please note, is not included in the calculation as it does not form part of our laboratory database. 7ZA2FH AAKUWE [Participant created a manually formatted table within the free form text space. This special formatting was not transferable into the final report. Data is presented as is.] U=Half Brother A=Half Sister U/?:M+F A:M+X Locus STR Half Brother Half Sister Formula Given frequency LR (p+u+4pu) / 8pu p=12, u= ,18 18,24 (1+4q) / 8q q= D2S44 (1+4q) / 8q q= D3S ,17 (1+4p) / 8p p= D5S818 11,11 (1+2p) / 4p p= ,11 8,11 (p+u+4pu) / 8pu p=8, u= D8S ,1 (1+4p) / 8p p= D10S1248 (1+2p) / 4p p= D12S391 17,22 18,19 1/ ,11 8,12 (1+4p) / 8p p= D1S539 9,12 (1+4s) / 8s s= D18S51 13,17 12,1 1/ D19S433.2 (1+4p) / 8p p= D21S11 27,28 28,32.2 (1+4q) / 8q q= D22S ,15 15,15 (1+2p) / 4p p= AMEL CSF1PO 11,11 11,11 (1+p) / 2p p= ,2 18,2 (p+u+4pu) / 8pu p=18,u= PENTA D 9,9 9,12 (1+2p) / 4p p= PENTA E 7,8 7,11 (1+4p) / 8p p= ,17 17,20 (1+4p) / 8p p= ,9 9,9.3 (1+4s) / 8s s= ,9 8,8 (1+2p) / 4p p= VWA 14,17 (1+4p) / 8p p= cumulative LR= Probability of Sibship= LR/(LR+1)X 100% = /( )X 100% = % [Participant created a manually formatted table within the free form text space. This special formatting was not transferable into the final report. Data is presented as is.] Item 3: Autosomal STRs: The DNA profile is single source. The alleged father, Subject Subject, cannot be excluded as the potential biological father of the child, Child Victim, using Autosomal STRs. These profiles are "X' times more likely to occur if Child Victim is the child of Victim Victim and Subject Subject than if Child Victim is the child of Victim Victim and a random person from the reference populations listed where "X" equals: African American 820 BILLION; Caucasian 42 BILLION; Hispanic 1.2 TRILLION. Y STRs: The DNA profile is single source. The alleged father, Subject Subject, cannot be excluded as the potential biological father of Child Victim using YSTRs. These profiles are "X" times more likely to occur if the abovereferenced individuals (or their patrilineal relative(s)) are the contributors than if the source of the evidence is a random, unrelated person from the reference populations listed where "X" equals*: African American 1852; Caucasian 215; Hispanic *Numbers are based upon the US YSTR database and a 95% confidence limit. KINSHIP DNA STATISTICS (NONPARENTAGE) Half Brother and Half Sister: Autosomal STRs: The DNA profile is single source. The kinship index supports the hypothesis that Profile B (Half Sister) is the half sibling of Profile A (Half Brother) using the reference populations listed. The genotype observed for Profile B is "X" times more likely to occur in a half sibling of Profile A than in someone unrelated to Profile A from the reference populations listed where "X" equals: African American 30 THOUSAND; Caucasian 2.4 THOUSAND; Hispanic 57 THOUSAND ( 48 )

49 DNA Parentage Test TABLE 8 Additional Statistical Results CRZKB [Participant created a manually formatted table within the free form text space. This special formatting was not transferable into the final report. Data is presented as is.] U = Half brother A = Half sister U/?:M+F A:M+Y Locus STR Half Brother Half Sister Formula Given frequency LR (p+u+4pu) / 8pu p=12, u= ,18 18,24 (1+4q) / 8q q= D2S44 (1+4q) / 8q q= D3S ,17 (1+4p) / 8p p= D5S818 11,11 (1+2p) / 4p p= ,11 8,11 (p+u+4pu) / 8pu p=8, u= D8S ,1 (1+4p) / 8p p= D10S1248 (1+2p) / 4p p= D12S391 17,22 18,19 1/ ,11 8,12 (1+4p) / 8p p= D1S539 9,12 (1+4s) / 8s s= D18S51 13,17 12,1 1/ D19S433.2 (1+4p) / 8p p= D21S11 27,28 28,32.2 (1+4q) / 8q q= D22S104511,15 15,15 (1+2p) / 4p p= AMEL CSF1PO 11,11 11,11 (1+p) / 2p p= ,2 18,2 (p+u+4pu) / 8pu p=18,u= PENTA D 9,9 9,12 (1+2p) / 4p p= PENTA E 7,8 7,11 (1+4p) / 8p p= ,17 17,20 (1+4p) / 8p p= ,9 9,9.3 (1+4s) / 8s s= ,9 8,8 (1+2p) / 4p p= VWA 14,17 (1+4p) / 8p p= cumulative LR= Probability of Sibship= / x = % DGEVP4 ERQMR4 G9CPNY JNGXGX Genetic evidence suports the hypothesis that they are rather full siblings of Caucasian origin: LR=7,5E+4, LR for half siblings of Caucasian origin amounts 1,1E+4. Genetic evidence suports the hypothesis that they are rather full siblings of AfroAmerican origin: LR=5,14E+, LR for half siblings of Caucasian origin amounts 1,4E+.Genetic evidence suports the hypothesis that they are rather full siblings of Asian origin: LR=5,52E+5, LR for half siblings of Asian origin amounts 7,79E+5. Based on the performed calculations the most likely is hipothesis that the examined persons are full siblings of Caucasian origin. Probability= % Half Sibling profiles were compared by using the Caucasian population database in previous sections. Theses two DNA profiles are likely to be biologically related as half sibling (probability of brotherhood = 99.98%). [Participant created a manually formatted table within the free form text space. This special formatting was not transferable into the final report. Data is presented as is.] U=half brother A=half sister U/?:M+F A:M+X Locus STR Half Brother Half Sister Formula Given frequency LR (p+u+4pu)/8pu p=12,u= ,18 18,24 (1+4q)/8q q= D2S44 (1+4q)/8q q= D3S ,17 (1+4p)/8p p= D5S818 11,11 (1+2p)/4p p= ,11 8,11 (p+u+4pu)/8pu p=8,u= D8S ,1 (1+4p)/8p p= D10S1248 (1+2p)/4p p= D12S391 17,22 18,19 1/ ,11 8,12 (1+4p)/8p p= D1S539 9,12 (1+4s)/8s s= D18S51 13,17 12,1 1/ D19S433.2 (1+4p)/8p p= D21S11 27,28 28,32.2 (1+4q)/8q q= D22S ,15 15,15 (1+2p)/4p p= AMEL XY XX CSF1PO 11,11 11,11 (1+p)/2p p= ,2 18,2 (p+u+4pu)/8pu p=18,u= PENTA D 9,9 9,12 (1+2p)/4p p= PENTA E 7,8 7,11 (1+4p)/8p p= ,17 17,20 (1+4p)/8p p= ,9 9,9.3 (1+4s)/8s s= ,9 8,8 (1+2p)/4p p= VWA 14,17 (1+4p)/8p p= Cumulative LR= Probability of sibship=lr/(lr+1)*100 =(( / ))*100 = % ( 49 )

50 DNA Parentage Test TABLE 8 Additional Statistical Results KNB49B Data recorded above are calculated with the NIST database. Probability of relationship is 99.98%. Inhouse calculation of such a scenario would make use of inhouse database frequencies, which are based on the FBI information available in Brenner's DNAView program. Using those frequencies, the kinship index would be reported as , yielding a probability of a half sibling relationship of 99.97% (prior = 0.5). NVZV92 QHP2UT R7QKHX RCVLHR T4TUM3 VGU8YK WNA8TM YYKJYG ZDF4DJ ZHCMFG The kinship index supports the hypothesis that Half Sister is the half sibling of Half Brother using the reference populations listed. The genotype observed for Half Sister is X times more likely to occur in a half sibling of Half Brother than in someone unrelated to Half Brother from the reference populations listed where X equals: African American 30 thousand; Caucasian 2.4 thousand; Hispanic 57 thousand Half Sibling Kinship Index = 9, Posteria Probability = % Probability = %. The kinship index supports the hypothesis that Item 001.C is the half sister of Item 001.B using the reference populations listed. The genotype observed for Item 001.C is X times more likely to occur in a half sister of Item 001.B than in someone unrelated to Item 001.B from the reference populations listed where X equals: African American 30 THOUSAND; Caucasian 2.4 THOUSAND; Hispanic 57 THOUSAND By using the population database for caucasian. There is greater probability they are half siblings HSS (Half Siblings, One of each s parents are halfsiblings to each other). No conclusion could be made as to whether the two individuals are half siblings or not When comparing the male profile "Half brother" and the female profile "Half sister", a kinship index of 9318 and a probability of brotherhood of 99.99% were obtained. According to results obtained by DNAVIEW software, the kinship analysis better supports the hypothesis of full siblings instead of half siblings as suggested by the question posed (full siblings kinship index = 57540), but in any case there is a genetic evidence supporting the relationship between these two subjects. Two DNA profiles were compared by using the Caucasian population database same as previous section. There are likely to be half siblings relationship because a probability of kinship index is greater than 99.88% For scenario: pq, pq: LR = (p+q+4pq)/8pq. pq, pr: LR = (1=$p) / 8p. pq, pp: LR = (1+2p) / 4p. pq, rs: LR = 0,5. pp, pp: LR = 2p(1+p) / (2pp) Such service is not provided. ( 50 )

51 DNA Parentage Test BPGWF Additional Comments TABLE 9 Additional Comments PowerPlex Fusion and AmpFlstr YFiler were performed on items 2 and 3. Results were concordant at DYS391. 7FFFB 7ZA2FH 9VQKZA A2B3UC CRZKB D3UFL DGEVP4 G9CPNY JNGXGX N7ZHZX DYS391 locus was not available in the PowerPlex Fusion amplification kit to report the allele calls. The allele call at DYS391 was found to be concordant in the YFiler and PowerPlex Fusion kits for Item 2 and Item On comparison of the DNA profiles, I found that the source of bloodstained specimen Item 3 is the biological father to the source of bloodstained specimen Item 2 (given that the biological mother is represented by the source of bloodstained specimen Item 1 ). 2. Item 1, Item 2 and Item 3 were extracted insitu method and amplified using AmpFISTR Direct Kit. 3. YSTR analysis was carried out on Item 2 and Item Electrophoresis were carried out using Applied Biosystem 3130xL Genetic Analyzer. 5. Reagent blank, positive and negative control were carried out along with the analysis and gave designated result.. The statistical formula were derived by DNA View Statistical Software and calculated using Microsoft Excel. PowerPlex Fusion and YFiler results were concordant at DYS391 for samples 02A and 03A. PART III KINSHIP DNA STATISTICS (NONPARENTAGE) NOT APPLICABLE TO THIS LABORATORY. 1. I found that the donor of bloodstained specimen Item 3 is the biological father to the donor of bloodstained specimen Item 2 (given that the biological mother is represented by "Item 1"). 2. Item 1, item 2 and item 3 were extracted using insitu method and amplified using AmpFISTR Direct Kit. 3. YSTR analysis was conducted on Item 2 and item Electrophoresis were carried out using Applied Biosystem 3130xL Genetic Analyzer. 5. Reagent blank, positive and negative control were carried out along with the analysis.. The statistical formula were derived by DNA View Statistical Software and calculated using Microsoft Excel. Item 2 demonstrated concordance at DYS391 in PowerPlex Fusion and YFiler. Item 3 demonstrated concordance at DYS391 in PowerPlex Fusion and YFiler. Paternity DNA: There is 2,5E+13 times more probable to obtain the genetic results (determined DNA profiles of Mother, her Son and alleged Father) given that the alleged father is the biological father of son than to obtain the same genetic results if the examined trio are unrelated. The Alleged parent (Item 3) could not be excluded as the biological parent of the child (Item 2) based on the DNA results tested. 1. I found that the donor of bloodstained specimen Item 3 is the biological father to the donor of bloodstained specimen Item 2 (given that the biological mother is represented by "Item 1"). 2. Item 1, item 2 and item 3 were extracted using insitu method and amplified using AmpFISTR Direct Kit. 3. YSTR analysis was conducted on Item 2 and item Electrophoresis were carried out using Applied Biosystem 3130xL Genetic Analyzer. 5. Reagent blank, positive and negative control were carried out along with the analysis.. The statistical formula were derived by DNA View Statistical Software and calculated using Microsoft Excel. Powerplex 21 [Country] Caucasian Dataset used, using formula of Balding and Nichols with Theta = Assuming mother and alleged father both from this population. Powerplex 21 [Country] Caucasian Dataset also used for kinship index, also considering Theta = 0.02 and formula of Balding and Nichols. Suggestion: It would be helpful if a value was given for theta to use in these calculations to allow comparison between laboratories. ( 51 )