INDIAN RIVER CRIME LABORATORY

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1 03166 INDIAN RIVER CRIME LABORATORY at INDIAN RIVER STATE COLLEGE 4602 KIRBY LOOP ROAD FT. PIERCE, FL August 2, 2016 The Honorable Sheriff Ken Mascara St. Lucie County Sheriffs Office 4700 W. Midway Rd. Ft. Pierce, FL Case Officer: Robert P. Barton Lab Case#: Report #: 0004 Agency Case #: SUBJECT: Holmes, Keith SUBJECT: MacNaught, Brian DECEASED: Semer, Demarcus C. EVIDENCE SUBMITTED The Results and Remarks are the conclusions, opinions, and/or interpretations of the analyst whose signature appears on the report. On 5/10/2016, Blood Alcohol Analyst Robert Parsons, Jr. received the following evidence from the St. Lucie County Sheriffs Office via Amanda Rodriguez: PURPOSE Exhibit# : Exhibit# : One sealed manila envelope containing one large plastic zip-lock bag with some plant matter. One sealed cardboard box containing multiple vacuum filters. To determine the presence or absence of substances controlled under sections Florida Statutes, the Florida Comprehensive Drug Abuse Prevention and Control Act. EXAMINATIONS PERFORMED Exhibit # Duquenois-Levine Macromorphology Microscopy (stereo reflection) NFLIS Report Exhibit # Duquenois-Levine Macromorphology Microscopy (stereo reflection) NFLIS Report RESULTS Exhibit # Cannabis, Schedule I, net: 8.68 grams Exhibit # Cannabis, Schedule I, net: 0.01 grams REMARKS Cannabis (marijuana) is a naturally occurring plant with hallucinogenic properties, and is controlled under Florida Statutes Chapter 893 (2013) as a Schedule I substance (893.03(1)(c)7). v Page: 1 of 2

2 7Labor~tory Report, Continued 8/2/16"' Please be advised only one filter was analyzed in Exh # The submitting authority is requested to pick up the exhibit(s) at their earliest opportunity. CC: State Attorney's Office v Page: 2 of 2 Analyst's initials 03167

3 03168 INDIAN RIVER CRIME LABORATORY at INDIAN RIVER STATE COLLEGE 4602 KIRBY LOOP ROAD FT. PIERCE, FL July 13, 2016 Marl< A. Chapman Criminalist The Honorable Sheriff Ken Mascara St. Lucie County Sheriff's Office 4700 W. Midway Rd. Ft. Pierce, FL Case Officer: Robert P. Barton Lab Case#: Report#: Agency Case #: SUBJECT: Holmes, Keith SUBJECT: MacNaught, Brian DECEASED: Semer, Demarcus C. EVIDENCE SUBMITTED The Results and Remarks are the conclusions, opinions, and/or interpretations of the analyst whose signature appears on the report. On 5/10/2016, Blood Alcohol Analyst Robert Parsons; Jr. received the following evidence from the St. Lucie County Sheriff's Office via Amanda Rodriguez: Exhibit# : Exhibit# : Exhibit# : Exhibit# : Exhibit# : Exhibit# : Exhibit# : Exhibit# : Exhibit# : Exhibit# : Exhibit# : Exhibit# : Exhibit# : Exhibit# : Sealed manila envelope containing an unsealed 35mm film canister, containing a bullet. Sealed manila envelope containing an unsealed 35mm film canister, containing a bullet fragment. Sealed white envelope containing a gray short-sleeved tee shirt. Sealed.manila envelope containing a sealed smaller envelope, containing a bullet. Sealed manila envelope containing an unsealed 35mm film canister, containing a bullet fragment. v Page: 1 of 2

4 03169 July 13, 2016 Exhibit# :, Continued Sealed manila envelope containing an unsealed 35mm film canister, containing a bullet fragment. Exhibit# : Sealed manila envelope containing an unsealed 35mm film canister, containing a bullet. On 6/7/2016, Blood Alcohol Analyst Robert Parsons, Jr. received the following evidence from the St. Lucie County Sheriff's Office via RENEE PIENIAZEK: Exhibit# : Sealed manila envelope containing three bullets and three cartridge cases test fired in the firearm in Exhibit # Exhibit# : Sealed manila envelope containing three bullets and three cartridge cases test fired in the firearm in Exhibit # PURPOSE: Function examination Comparison Gunshot residue testing RESULTS The firearms in Exhibits # and # were examined for functional defects and test fired. No functional defects were found with either one. The cartridge cases in Exhibits # through # were identified as having been fired in the firearm in Exhibit # The cartridge cases in Exhibits # through # and the bullet fragment in Exhibit # were identified as having been fired in the firearm in Exhibit # The bulleubullet fragments in Exhibits #223256, #223259, #223270, # and # could not be identified as having been fired in the firearm in Exhibit # or the firearm in Exhibit # However, similar class characteristics (caliber and rifling) indicate that they could have been fired in the firearm in Exhibit # or the firearm in Exhibit # The area around the hole in the right middle back of the tee shirt in Exhibit # was microscopically examined and chemically processed for the presence of gunshot residues and none were found. REMARKS The firearms designated as Exhibits # and # were delivered to the Indian River Crime Laboratory by a representative of the St. Lucie County Sheriff's Office for function testing and test firing. The representative was present during the entire examination process and transported the Exhibits along with the generated test fires back to their agency. The test fires were later submitted for comparison purposes as Exhibits # (from the firearm in Exhibit #222766) and # (from the firearm in Exhibit # ). A blood-like substance was visible on the bullet in Exhibit # The submitting authority is requested to pick up the exhibit(s) at their earliest opportunity. CC: State Attorney' s Office v Page: 2 of 2 1~ Analyst Initials

5 03170 INDIAN RIVER CRIME LABORATORY at INDIAN RIVER STATE COLLEGE 4602 KIRBY LOOP ROAD FT. PIERCE, FL June 21, 2016 Submitted by: w w~ wai j.,.wafker Criminalist The Honorable Sheriff Ken Mascara St. Lucie County Sheriff's Office 4700 W. Midway Rd. Ft. Pierce, FL Case Officer: Robert P. Barton Lab Case#: Report#: Agency Case #: SUBJECT: Holmes, Keith SUBJECT: MacNaught, Brian DECEASED: Semer, Demarcus C. EVIDENCE SUBMITTED The Results and Remarks are the conclusions, opinions, and/or interpretations of the analyst whose signature appears on the report. On 5/10/2016, Blood Alcohol Analyst Robert Parsons, Jr. received the following evidence from the St. Lucie County Sheriff's Office via Amanda Rodriguez: Exhibit# : One sealed manila envelope containing an oral standard from Brian MacNaught Exhibit# : Exhibit# : Exhibit# : Exhibit# : Exhibit# : Exhibit# : One sealed manila envelope containing an oral standard from Keith Holmes One sealed manila envelope containing a piece of plastic bag One sealed manila envelope containing a bloodstain card from Demarcus Semer One sealed manila envelope containing swabs of back door step One sealed paper bag containing denim pants & belt One sealed manila envelope containing swabs of a palm print, windshield frame driver's side Exhibit# : One sealed manila envelope containing swabs of a palm passenger window print, front Exhibit# : Exhibit# : Exhibit# : Exhibit# : Exhibit# : v One sealed manila envelope containing swabs of fingerprints, upper rear passenger side quarter panel One sealed manila envelope containing swabs of fingerprint, front passenger window One sealed manila envelope containing swabs of a palm print, hood One sealed manila envelope containing swabs of a smear, rear driver's side window One sealed manila envelope containing swabs from above front passenger door Page: 1 of 5

6 June 21, 2016, Continued Exhibit# : Exhibit# : Exhibit# : Exhibit# : Exhibit# : Exhibit# : Exhibit# : Exhibit# : Exhibit# : Exhibit# : Exhibit# : Exhibit# : Exhibit# : Exhibit# : Exhibit# : Exhibit# : Exhibit# : PURPOSE Forensic Biology Examinations One sealed manila envelope containing swabs of driver's door window frame One sealed manila envelope containing swabs of a fingerprint, front passenger door One sealed manila envelope containing swabs of front passenger window One sealed manila envelope containing swabs of front passenger window frame One sealed manila envelope containing swabs of a fingerprint, front passenger window frame One sealed manila envelope containing swabs of a fingerprint, passenger side window midsection One sealed manila envelope containing swabs of a fingerprint, passenger side window far right One sealed manila envelope containing swabs of driver's side door panel top One sealed manila envelope containing swabs of driver's side door arm rest One sealed manila envelope containing swabs of driver's side door lock One sealed manila envelope containing swabs of passenger door panel top One sealed manila envelope containing swabs of passenger side door arm rest One sealed manila envelope containing swabs of gear shift One sealed manila envelope containing swabs of center console One sealed manila envelope containing swabs of a fingerprint, passenger window upper front One sealed manila envelope containing swabs of a fingerprint, driver's side window One sealed manila envelope containing swabs of a fingerprint, driver's side window RESULTS The back door step swabs (Exhibit ) and denim pants & belt (Exhibit ) tested positive chemically (phenolphthalein) indicating the presence of blood. A sample from the piece of plastic bag (Exhibit ) was collected in an attempt to determine the possible handler of this exhibit. v Page: 2 of 5 Analyst's Initials 03171

7 June 21, 2016, Continued DNA isolated from the swabs of a palm print, windshield frame driver's side (Exhibit ), swabs of a palm print, front passenger window (Exhibit ), swabs of fingerprints, upper rear passenger side quarter panel (Exhibit ), swabs of fingerprint, front passenger window (Exhibit ), swabs of a palm print, hood (Exhibit ), swabs of a smear, rear driver's side window (Exhibit ), swabs from above front passenger door (Exhibit ), swabs of driver's door window frame (Exhibit ), swabs of a fingerprint, front passenger door (Exhibit ), swabs of front passenger window (Exhibit ), swabs of front passenger window frame (Exhibit ), swabs of a fingerprint, front passenger window frame (Exhibit ), swabs of a fingerprint, passenger side window midsection (Exhibit ), swabs of a fingerprint, passenger side window far right (Exhibit ), swabs of driver's side door panel top (Exhibit ), swabs of driver's side door arm rest (Exhibit ), swabs of driver's side door lock (Exhibit ), swabs of passenger door panel top (Exhibit ), swabs of passenger side door arm rest (Exhibit ), swabs of gear shift (Exhibit ), swabs of center console (Exhibit ), swabs of a fingerprint, passenger window upper front (Exhibit ), swabs of a fingerprint, driver's side window (Exhibit ), swabs of a fingerprint, driver's side window (Exhibit ), swabs of back door step (Exhibit ), a piece of plastic bag (Exhibit ), oral standard from Brian MacNaught (Exhibit ), oral standard from Keith Holmes (Exhibit ), and bloodstain card from Demarcus Semer (Exhibit ) was amplified by the polymerase chain reaction (PCR) and characterized at the following loci: D8S1179, D21 S11, D7S820, CSF1 PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vwa, TPOX, D18S51, D5S818, FGA, and the gender-determining marker Amelogenin. No further analysis was performed on the denim pants & belt (Exhibit ). v Page: 3 of 5 Analyst's Initials 03172

8 June 21, 2016, Continued REMARKS Complete DNA profiles were obtained from the oral standard from Brian MacNaught (Exhibit ), oral standard from Keith Holmes (Exhibit ), and bloodstain card from Demarcus Semer (Exhibit ). These DNA profiles have been entered into the Combined DNA Index System (CODIS) and will be periodically searched against the appropriate indexes, with any matches being reported. Inconclusive DNA profiles were obtained from the following exhibits which are not suitable for comparison due to insufficient DNA quality and/or quantity: swabs of driver's side door panel top (Exhibit ) swabs of driver's side door arm rest (Exhibit ) swabs of passenger door panel top (Exhibit ) swabs of passenger side door arm rest (Exhibit ) swabs of center console (Exhibit ) Insufficient DNA/data was obtained from the following exhibits which are not suitable for comparison due to insufficient DNA quality and/or quantity: swabs of a palm print, windshield frame driver's side (Exhibit ) swabs of a smear, rear driver's side window (Exhibit ) swabs of driver's door window frame (Exhibit ) swabs of a fingerprint, passenger side window midsection (Exhibit ) swabs of a fingerprint, driver's side window (Exhibit ) swabs of a fingerprint, driver's side window (Exhibit ) piece of plastic bag (Exhibit ) No DNA profile was obtained from the following exhibits. Insufficient DNA quality and/or quantity can affect the ability to generate a DNA typing result: swabs of a palm print, front passenger window (Exhibit ) swabs of fingerprints, upper rear passenger side quarter panel (Exhibit ) swabs of fingerprint, front passenger window (Exhibit ) swabs of a palm print, hood (Exhibit ) swabs from above front passenger door (Exhibit ) swabs of a fingerprint, front passenger door (Exhibit ) swabs of front passenger window (Exhibit ) swabs of front passenger window frame (Exhibit ) swabs of a fingerprint, front passenger window frame (Exhibit ) swabs of a fingerprint, passenger side window far right (Exhibit ) swabs of a fingerprint, passenger window upper front (Exhibit } A mixed DNA profile was obtained from the swabs of driver's side door lock (Exhibit ). Demarcus Semer (Exhibit ) is included as a possible contributor to this mixture. For the loci D8S1179, D21S11, CSF1PO, D3S1358, TH01, D13S317, D16S539, D19S433, vwa, and TPOX, the probability that a randomly chosen, unrelated individual would be included as a possible contributor to this mixture is 1 in 182,800 using an African American database. Brian MacNaught (Exhibit ) and Keith Holmes (Exhibit ) are excluded as possible contributors to this mixture. A mixed DNA profile was obtained from the swabs of gear shift (Exhibit ). The male DNA profile obtained from the major component of this mixture matches the DNA profile obtained from the bloodstain card from Demarcus Semer (Exhibit ) at nine loci; comparisons at the remaining six loci (CSF1 PO, D2S1338, D19S433, vwa, D18S51, and FGA) were inconclusive due to insufficient DNA quantity and/or quality. For the loci D8S1179, D21S11, D7S820, D3S1358, TH01, D13S317, D16S539, TPOX, and D5S818, the frequency with v Page: 4 of 5 Analyst's Initials 03173

9 June 21, 2016, Cantin ued which the major evidence DNA profile is expected to be found in an unrelated individual in the population at large is 1 in 6,583,000,000 using an African American database. Comparisons to the remainder of DNA present in this mixture sample can not be performed due to limited DNA quality and/or quantity. A complete male DNA profile was obtained from the swabs of back door step (Exhibit ). This DNA profile matches the DNA profile obtained from the bloodstain card from Demarcus Semer (Exhibit ) at all fifteen loci. To a reasonable degree of scientific certainty, in the absence of an identical twin, Demarcus Semer (Exhibit ) is the source of the DNA profile obtained from ~he swabs of back door step (Exhibit ). Portions of the swabs of a palm print, windshield frame driver's side (Exhibit ), swabs of a palm print, front passenger window (Exhibit ), swabs of fingerprints, upper rear passenger side quarter panel (Exhibit ), swabs of fingerprint, front passenger window (Exhibit ), swabs of a palm print, hood (Exhibit ), swabs of a smear, rear driver's side window (Exhibit ), swabs from above front passenger door (Exhibit ), swabs of driver's door window frame (Exhibit ), swabs of a fingerprint, front passenger door (Exhibit ), swabs of front passenger window (Exhibit ), swabs of front passenger window frame (Exhibit ), swabs of a fingerprint, front passenger window frame (Exhibit ), swabs of a fingerprint, passenger side window midsection (Exhibit ), swabs of a fingerprint, passenger side window far right (Exhibit ), swabs of driver's side door panel top (Exhibit ), swabs of driver's side door arm rest (Exhibit ), swabs of driver's side door lock (Exhibit ), swabs of passenger door panel top (Exhibit ), swabs of passenger side door arm rest (Exhibit ), swabs of gear shift (Exhibit ), swabs of center console (Exhibit ), swabs of a fingerprint, passenger window upper front (Exhibit ), swabs of a fingerprint, driver's side window (Exhibit ), swabs of a fingerprint, driver's side window (Exhibit ), swabs of back door step (Exhibit ), oral standard from Brian MacNaught (Exhibit ), oral standard from Keith Holmes (Exhibit ), and bloodstain card from Demarcus Semer (Exhibit ), as well as any remaining DNA extracts, are being retained by the Indian River Crime Laboratory. The submitting authority is requested to pick up the exhibit(s) at their earliest opportunity. CC: State Attorney's Office v Page: 5 of 5 Analyst's Initials 03174

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