Accessories Handbook. for NanoWizard, ForceRobot and CellHesion. systems. valid from Unlimited possibilities.

Transcription

1 Accessories Handbook for NanoWizard, ForceRobot and CellHesion systems Unlimited possibilities. valid from 2012

2 The NanoWizard, ForceRobot and CellHesion family Optional heads CellHesion 200 CellHesion 200 ForceRobot 300 ForceRobot 300 Head NanoWizard 3 NanoWizard 3 NanoScience and BioScience versions NanoWizard 3 NanoWizard 3 NanoOptics fiber port and UV transparency versions Vortis SPMControl Vortis SPMControl Vortis Advanced SPMControl SPMControl Control electronics options Signal Access Module Signal Access Module (SAM) (SAM) Software SPM Control & DATA SPM Control & DATA Processing Processing Experiment Planner Manipulation & lithography Direct Overlay Advanced Force Spectroscopy QI Advanced System options TAO CellHesion BioMAT Module Module TopView Optics PetriDishHeater for BioMAT 3-axes 2-axes Stages Motorized precision stage Life sciences stage Standard stage HCS Heating cooling stage ECCell Electrochemisty cell Temperature control PetriDishHeater BioCell ECCell Electrochemistry cell HCM Heating cooling module HTHS High temperature heating stage CoverslipHolder Fluid cells & cantilever holders SmallCell Small volume SmallCell Standard CantileverHolder Supercut CantileverHolder Magnetic field CantileverHolder Side-view CantileverHolder CantileverHolder with electrical tip connection Electrical modes & application modules STM module High Voltage Sample Bias Amplifier Conductive AFM module Kelvin Probe module Tuning fork module HyperDrive fluid imaging package CoverslipHolder with electrical sample connection Extras & OEM 8x FluidicsModule Acoustic enclosure Vibration isolation Base frame for active isolation tables Fiber coupled detection module Cameras Setups and accessories for BioScience applications highlighted with LIGHT BLUE BOXES Typical BioScience applications and research fields: High resolution imaging in air and liquid of single molecules such as proteins, DNA/RNA, carbohydrates, lipids, Live Cell Imaging, bacteria and virus characterization, cellular adhesion and cytomechanics, molecular interactions, receptor-ligand, antibody-antigen, molecular unfolding, molecular recognition, biomaterials, compound and implant characterization, material properties such as adhesion, elasticity, hardness; drug formulation, encapsulation and aging studies and many more Setups and accessories for NanoScience applications highlighted with GRAY BOXES Typical NanoScience applications and research fields: High resolution imaging in air and liquid of single molecules, thin films, nanoparticles or nanowires, surface properties characterization such as adhesion, stiffness, friction, conductivity, charges, hardness; polymer science, single molecule force measurements, smart organic materials, surface properties of conjugated polymers, biosensors, degradable materials, electrochemistry, colloidal probe, surface forces and interface phenomena; and many more For more detailed information about all options see the brochures and product notes or visit 2

3 Accessories Handbook Contents 1 Control electronics options 4 Vortis SPMControl Station Vortis Advanced SPMControl Station Signal Access Module (SAM) 2 System options 5 NanoWizard 3 NanoOptics version head ForceRobot 300 CellHesion 200 TAO Tip Assisted Optics module BioMaterials Workstation BioMAT CellHesion module 3 Software modules 7 DirectOverlay module NanoLithography/NanoManipulation module Advanced Force Spectroscopy module QI Advanced software module ExperimentPlanner software module 4 Applications modules and modes 8 HyperDrive fluid imaging package Electrical sample connection module CoverslipHolder with electrical sample connection Kelvin Probe (KPM) and Scanning Capacitance (SCM) Microscopy module Conductive AFM module Conductive AFM module Enclosed Volume Scanning Tunnelling Microscopy (STM) module Tuning Fork module High Voltage Sample Bias Amplifier ForceWheel 5 Sample stages 10 Motorized precision stage Manual precision stage Sample holder for large samples Standard stage 6 Cantilever holder options 11 Standard cantilever holder Supercut version cantilever holder Cantilever holder with electrical tip connection Side-view cantilever holder Magnetic field cantilever holder 7 Environmental control options fluid cells and temperature controls 12 BioCell CoverslipHolder PetriDishHeater PetriDishHeater for BioMAT SmallCell 3-port small volume SmallCell High Temperature Heating Stage (HTHS ) Heating Cooling Module (HCM ) Heating Cooling Stage (HCS ) 8 Electrochemistry solutions 14 Temperature controlled ECCell Heating Cooling Stage (HCS ) with electrochemistry cell OEM Potentiostats from edaq 9 Vibration and acoustic isolation 15 Active vibration isolation from Accurion Active vibration isolation from Table Stable Active air damping tables from TMC JPK acoustic enclosure JPK base for acoustic enclosure 10 Optics accessories 16 TopViewOptics TopViewOptics with boom stand TopViewOptics for use with inverted optical microscopes or the JPK BioMAT Fiber coupled detection module Inverted optical microscope configurations 11 OEM solutions and small parts 17 JPK sample holder for small samples JPK cable anchorage pillow JPK cantilever changing tool JPK biocompatible glue JPK spare parts OEM syringe pumps JPK 8x FluidicsModule AFM cantilevers, substrates and standards CCD cameras OEM Scanning Thermal AFM from Anasys OEM Indentation solutions OEM incubators for Live Cell experiments under controlled environments OEM glove box system for NanoWizard AFMs 3

4 1 Control electronics options Vortis SPMControl Station State-of-the-art digital controller with lowest noise levels. High speed 16bit AD conversion with 60 MHz for the photodetector signals 24 bit ultra precise ADC with 2.5 MHz High speed lock-in amplifier technology for precise amplitude and phase detection High speed data capture with optional burst mode Modular hybrid analog/digital design with latest FPGA/PPC technology MHz) Gigabit Ethernet interface for fast data link Number of data points that can be captured continuously: restricted only by HDD Thermal noise acquisition up to 3.25 MHz Vortis Advanced SPMControl Station The most advanced SPM controller. (see product note) State-of-the-art digital controller with lowest noise levels and highest number of signal channels 4 high speed 16 bit ADC channels with 60 MHz 12 ADCs with 18 bit with 800 khz 24 bit ultra precise ADC with 2.5 MHz 3 channels 14 bit DAC with 120 MHz 8 channels 24 bit DAC with 400 khz 2 high-speed and 1 mid-speed lock-in amplifiers for precise amplitude and phase detection High speed data capture Modular hybrid analog/digital design with latest FPGA/PPC technology 660 MHz / 240 MHz) Gigabit Ethernet interface for fast data link Number of data points that can be captured continuously: restricted only by HDD Thermal noise acquisition up to 3.25 MHz 2 gated photon counters Signal Access Module (SAM) Upgrade for the Vortis controller for more flexibility. Analog and digital connectors for maximum experimental freedom Analog inputs: 27; analog outputs: 23 Digital inputs: channels for, e.g., photon counting Digital outputs:

5 2 System options NanoWizard 3 NanoOptics head in two versions: with fiber port for fiber SNOM experiments UV transparency for top/down illumination The new NanoWizard 3 NanoOptics AFM head is optimized for a broad range of applications ranging from nanoscale optical imaging by aperture and scattering-type SNOM to experiments involving interactions of light with the sample such as absorption, excitation, nonlinear effects and quenching. (see NanoWizard 3 NanoOptics brochure) Comprehensive solutions for AFM and Raman spectroscopy, Tip-Enhanced Raman Spectroscopy (TERS), Aperture SNOM and Scattering-type SNOM (ssnom), Confocal microscopy, NanoManipulation in optical fields Compatible with most commercially available inverted research microscopes (Zeiss Axiovert and Axio Observer lines, Nikon TE and Ti lines, Olympus IX line and Leica DMI line) Unique integration with optical microscopy by tip and sample scanning design, DirectOverlay mode and smart engineering Seamless integration with inverted microscopes, Raman spectrometers, photon counting systems 980 nm laser source for cantilever deflection detection prevents cross talk with other wavelengths Wide range of operation modes and accessories such as Tuning Fork, STM, Conductive AFM, Fiber SNOM ForceRobot 300 The innovative force spectroscope with fully automated work flow. Take more than 80,000 force curves within 24 h while varying parameters such as temperature or loading rate. (see ForceRobot 300 brochure) Fully automated force spectroscope with highest flexibility Cutting edge force spectroscopy and force mapping in combination with single molecule fluorescence Automated laser and detector alignment Automated cantilever drift compensation Advanced and dedicated software with ExperimentPlanner, RampDesigner and built-in data batch processing Fits to the Vortis controller CellHesion 200 The single cell force testing solution for cell adhesion and elasticity studies. Specialized platform for cell adhesion and cell mechanics measurements from single molecules to entire cells. (see CellHesion 200 brochure) Cantilever sensor lifting system with >110 µm travel range with closed-loop control through high-speed capacitive sensor feedback Integrates with advanced optical imaging (DIC, CLSM, TIRF, FRET...) Fits to the Vortis controller 5

6 TAO Tip Assisted Optics module Specialized sample stage for advanced experiments combining AFM and optical spectroscopy based on proven NanoWizard technology. 2 axis and 3 axis version available. (see TAO module product note) Ultimate platform for TERS, scatter type SNOM, single molecule imaging/spectroscopy experiments NanoOptical studies such as quenching, field enhancement or bleaching Integrated sample scanning confocal imaging Integrates with conventional and advanced optical imaging (DIC, Phase contrast, CLSM, TIRF, FRET...) Ultimate flexibility through simultaneous control of up to 6 scan axes with closed loop precision (linearized with capacitive sensors): AFM tip scanner: µm 3 Sample scanner: µm µm µm 3 Flexible software system solution for experimental freedom and remote operation Fits to the Vortis Advanced controller BioMaterials Workstation BioMAT Completely integrated system comprising upright optical microscope and AFM. The specialist for investigations of opaque samples in life and material sciences. (see BioMAT brochure) Studies on non-transparent substrates or samples with AFM and optics Outstanding reproducibility of the focussed position (ROI) with both systems Unique capability to investigate the ROI precisely with optics and AFM Also perfect during operation in liquids Wide range of applications: Biochips, cell chips or patterned substrates for cell adhesion, plant cell applications, microbiology, tissue engineering,... Flexible concept with shuttle stage Both techniques can be operated even in different laboratory rooms CellHesion module As an add-on to the NanoWizard, the CellHesion module combines the capabilities of the BioAFM with precise adhesion force measurements and all optical microscopy features simultaneously. (see CellHesion module product note) 100 µm additional z-travel Perfect hardware linearization through capacitive sensors Simultaneous focus tracking optional Integrates with advanced optical imaging (DIC, Confocal microscopy, TIRF, FRET...) 6

7 3 Software modules DirectOverlay module Perfect integration of optical and AFM data JPK s proprietary and patented solution for perfect overlay of optical and AFM information. Perfect overlay of optical and AFM data with sub-diffraction limit precision Direct in optical image selection of AFM measurements (imaging and force curves) Dramatic reduction of overview image scanning in AFM, giving faster results & lower tip contamination Optical image navigation to specific regions of interest, even without AFM scanning. This protects functionalized tips for molecular recognition, avoiding tip passivation from image scanning before the force measurements. NanoLithography/NanoManipulation module Software module for NanoWizard systems. Free-hand or vector-based patterns are possible Moving objects or scratching the substrate with controlled force are possible Pattern creation by electrical current such as anodic oxidation 350nm Advanced Force Spectroscopy module For advanced force measurement experiments from single protein unfolding, DNA stretching to probing of cells and tissue. Integrated RampDesigner for user defined segmentation of force curves Advanced force clamp Tuneable frequency sweep from 0.1 to 10 khz Setting of user-defined patterns for rheology applications QI Advanced software module QI Advanced Software module for NanoWizard systems delivers quantitative mechanical properties. (see QI brochure) Any kind of sample can be imaged: samples with steep edges, loosely attached samples, soft, sticky and brittle samples Works under ambient conditions and in fluid Quick to learn and easy to operate Provides additionally adhesion, stiffness and dissipation data while scanning Depending on application, it can also deliver electrical conductivity or molecular recognition in a single scan ExperimentPlanner software module For comprehensive planning of an experiment with all of the external parameters. Delivers full control over all system options Design of advanced experiments Scanners, motors, light sources, detectors, environmental control options etc can be master controlled and timed 7

8 4 Applications modules and modes HyperDrive fluid imaging package For highest resolution imaging of small structured samples in liquid. (see jpk website for more information) Optional for Nanowizard 3 Nanoscience and Bioscience version Minimized tip-sample interaction by using smallest amplitudes Consists of a special cantilever holder with direct drive functionality, and a control software module Electrical sample connection module Accommodates electrical conductive samples such as AFM metal stubs by magnetic fixation or transparent samples such as ITO coated glass coverslips Insert for Life Science stages CoverslipHolder with electrical sample connection For electrical measurements such as Conductive AFM or STM on a coverslip in combination with high NA optics. Accommodates electrical conductive samples and transparent samples such as ITO coated glass coverslips Insert for Life Science stages Kelvin Probe (KPM) and Scanning Capacitance (SCM) Microscopy module Option for nanoscale mapping of surface potential distribution. Surface Potential Mapping Electrical tip connection External bias electronics module (+/-10 V) Software interface Conductive AFM module For conductivity and I/V measurements on the nanoscale. High-performance conductive AFM experiments Recording I/V curves Bias voltage range ± 10 V Noise limit 0.55 pa RMS Sample holders for opaque samples Sample holder for transparent samples works with all optical microscopes 8

9 Conductive AFM module Enclosed Volume For experiments in inert atmosphere; for conductivity and I/V measurements on the nanoscale under controlled environmental conditions. High-performance conductive AFM experiments under user defined gas atmosphere Recording I/V curves Bias voltage range ± 10 V Noise limit 0.55 pa RMS Sample holders for opaque samples Sample holder for transparent samples works with all optical microscopes Scanning Tunnelling Microscopy (STM) module The STM module fits directly to the NanoWizard head. The module can accommodate different STM probes Is compatible to the JPK electrical sample connection module Compatible with the JPK TopViewOptics for controlled tip positioning Tuning Fork module The Tuning Fork module fits directly to the NanoWizard head. Works with self excitation or external mechanical dither Allows vertical (shear force) or horizontal mounting of tuning forks Includes two holder boards and two unpackaged 40 khz tuning forks for self assembly High Voltage Sample Bias Amplifier Can be used for biasing a sample, e.g., in electro-optical experiments or in Piezo-Response Force Microscopy (PFM). +/- 50 V can be applied between sample and tip Software controlled ForceWheel JPK s ForceWheel handheld accessory for most sensitive experiment control, e.g., for force spectroscopy. Connected via USB Manual force curve acquisition Continuous setting of gains and setpoints Easy attachment of cells or beads with cantilever tip 9

10 5 Sample stages Motorized precision stage Offers automatic motion control for precise positioning of the sample relative to optical axis and AFM probe. Transmission illumination capability for inverted optical microscopes like Olympus IX 70/71 Zeiss Axio Observer/Axiovert 100/135/200 Nikon TE 2000 or Ti series Leica DMI 4000/5000 Please specify model upon order Travel range of the sample mm² Step size (Resolution): 1 µm Repeatability (unidirectional): 2 µm Maximum velocity: 1 mm/s With joystick or software control For automated tiling or mapping applications Manual precision positioning of the AFM tip Stand alone use or for inverted optical microscopes Compatible with all JPK add-ons, glass slides, Petri dishes etc. Compatible with NanoWizard II and 3, ForceRobot 300 and CellHesion 200 heads Manual precision stage Offers sub-micron resolution & fine motion control for precise positioning of the AFM tip relative to optical axis and the sample. Transmission illumination capability for inverted optical microscopes like Olympus IX 70/71 Zeiss Axio Observer/Axiovert 100/135/200 Nikon TE 2000 or Ti series Leica DMI 4000/5000 Please specify model upon order Precise positioning of the sample holder with thermally decoupled, magnetic fixation Flexible design for fluid cells, temperature control options, and custom sample holders Detachable from optical microscope for stand alone operation Drift compensated design Travel range of the sample mm² Compatible with NanoWizard II and 3, ForceRobot 300 and CellHesion 200 heads Sample holder for large samples The holder module fits directly to the manual or motorized precision stage. The holder can accommodate larger samples such as microchips or wafers and is equipped with spring clips which can be varied Standard stage Offers fine motion control for precise positioning of the AFM tip relative to the sample. Travel range of the AFM head (tip positioning) 10 10mm² Stand alone use in combination with JPK TopViewOptics Magnetic sample holder Flexible design for fluid cells and different sample holders Rigid, chemical resistive surface Maximum flexibility for customized sample mounting 10

11 6 Cantilever holder options Standard cantilever holder The standard holder for all-round applications with a large optical field of view. Chemically inert cantilever holder Optimized for the use with top-view optics Robust design using glass and medical steel or gold spring only Immersible to 8 mm fluid depth Easy to clean by detergents, ultrasonic bath or autoclaving Easy handling, even with gloves Measurements in droplets or in fluid baths like Petri dishes or home made fluid cells Works in air and liquid, including aggressive media Supercut version cantilever holder The holder is designed for use in air or liquids with removable cantilever spring for maximum cleaning results. Chemically inert cantilever holder For use with JPK BioCell, CoverslipHolder or PetriDishHeater for easier navigation Robust design using glass and medical steel or gold spring only Immersible to 8 mm fluid depth Easy to clean by detergents, ultrasonic bath or autoclaving Easy handling, even with gloves Measurements in droplets or in fluid baths like Petri dishes or home made fluid cells Works in air and liquid, including aggressive media Cantilever holder with electrical tip connection The holder is designed for use in air or liquids with a fixed cantilever spring and an electrical tip connection. For air or aqueous solutions Compatible with JPK BioCell, CoverslipHolder or PetriDishHeater for easier navigation Measurements in fluid droplets or in fluid baths like Petri dishes or home made fluid cells Robust design using glass and medical steel Immersible to 8 mm fluid depth Easy to clean Easy handling, even with gloves Electrical tip connection Side-view cantilever holder This holder is designed for use in combination with inverted optical microscopes to observe the cantilever region from the side. For use with JPK BioCell, CoverslipHolder or PetriDishHeater for easier navigation Measurements in fluid droplets or in fluid baths like Petri dishes or home made fluid cells Immersible to 8 mm fluid depth Easy to clean Easy handling, even with gloves Magnetic field cantilever holder This holder is designed for magnetic cantilever actuation. 11

12 7 Environmental control options fluid cells and temperature controls BioCell The BioCell allows for the temperature-dependent study of living cells or single molecules with the NanoWizard AFM in their native environment. It is therefore particularly suited for applications in cell biology and biomedical research. As an addon for inverted optical microscopes the NanoWizard AFM in combination with the BioCell offers the unique possibility to simultaneously study temperature-dependent processes like cell motility and metabolism with the AFM and simultaneous advanced optical imaging. During AFM measurement all the optical microscope s operation modes, in particular contrast techniques, remain constantly available. Even high-resolution fluorescence confocal microscopy and single-molecule fluorescence techniques are possible since objective lenses with highest numerical aperture can be used. Insert for Life Science stage Temperature range: 15 to 60 C Resolution: 0.1 C Quick-exchange cover slip bottom Connections for liquid perfusion and gas flow (e.g., CO 2 mix) Compatible to the NanoWizard AFM sample stage (Life Science version) Drift-minimized in all direction CoverslipHolder With the same design as the BioCell but without temperature control, the CoverslipHolder is the ultimate high resolution liquid cell with coverslip bottom. Insert for Life Science stage Closed liquid cell with standard coverslip bottom High-N.A. immersion optics possible by exchangeable cover slip bottom (0.17 mm thickness) Ports for perfusion & gas flow, e.g., CO 2 or nitrogen Superior design for combining AFM and confocal laser scanning Made of glass, steel or gold, and silicon only Easy to clean, also in ultrasonic bath Autoclavable inlet PetriDishHeater The heater system is fully compatible with JPK s range of NanoWizard AFM and CellHesion systems. This means all the standard imaging and measurement techniques may be applied at temperatures up to 60 C with 0.1 C precision. These include fluorescence, brightfield, phase contrast and DIC, AFM imaging and cell mechanics study. The system also offers perfusion control employing standard syringe pumps and gas flow of carbon dioxide possible for long term cell studies. Glass bottomed dishes are important for high numerical aperture fluorescence experiments such as confocal laser scanning used in combination with AFM. Insert for Life Science stage Temperature range: room temperature up to 60 C Compatible with mm Petri dishes from WPI and TPP - plastic or plastic with glass bottom Adaptors for other suppliers such as Matek, Willco, IWAKI, Ibidi, and BD upon request Ports for perfusion tubes and gas supply (only available for certain types of Petri dishes) Software control Temperature stability 0.1 K Fully compatible with JPK s range of NanoWizard AFM and CellHesion systems Fits to all inverted microscope brands in combination with a JPK sample stage 12

13 PetriDishHeater for BioMAT Shuttle stage with integrated Petri dish heater for JPK BioMaterials workstation. Temperature range: room temperature up to 60 C Compatible with mm Petri dishes from WPI and TPP plastic or plastic with glass bottom Dipping lenses need to be compatible with type of Petri dish Temperature stability 0.1 K Software control SmallCell The perfect solution for smallest volume experiments in a hermetically sealed environment, the SmallCell is perfect for fluid or gas exchange. The SmallCell is compatible with the JPK temperature control systems like the HTHS, HCM and the HCS. Solvent, acid and chloroform resistant Multi-purpose closed fluid cell for small volumes (< 150 µl) Inlet and outlet ports for perfusion experiments Glass, steel or gold, and resistive seal only Easy to clean, also in ultrasonic bath Autoclavable Temperature range up to 100 C 3-port small volume SmallCell The perfect solution for smallest volume experiments in a hermetically sealed environment, the small volume SmallCell is perfect for aqueous solutions exchange. The SmallCell is compatible with the JPK temperature control systems like the HTHS, HCM and the HCS. Closed fluid cell for minimized volumes (< 60 µl) Optimized for aqueous solutions Inlet and outlet ports for perfusion experiments with Luer connectors Temperature range up to 80 C Extra port for small amounts of liquids such as drugs, proteins etc. Polycarbonate, steel or gold, and seal only Easy to clean, also in ultrasonic bath Autoclavable High Temperature Heating Stage (HTHS ) This high performance heating stage is designed for demanding polymer applications as well as for stable long time temperature studies of single molecules or nanoparticles. Temperature range from ambient to 300 C in air and ambient to 80 C in fluid when used together with the JPK SmallCell Temperature resolution 0.1 C Drift minimized in all directions for full temperature ramps simultaneously with AFM imaging No water cooling or fan required Heating Cooling Module (HCM ) Designed for heating and cooling experiments in gas or liquids with minimized drift in all dimensions. Temperature range from -30 C to 120 C ~0-80 C temperature range for experiments in aqueous solutions Temperature resolution 0.1 C Compatible with JPK Manual precision stage and Motorized precision stage 13

14 Heating Cooling Stage (HCS ) Designed for AFM experiments in air or liquids from 0 C up to 100 C with minimized drift in all dimensions HCS follows the same design criteria as HTHS. Temperature range from 0 C to 100 C Temperature resolution 0.1 C Twin stage thermoelectric element Drift minimized in all directions for full temperature ramps simultaneous to AFM imaging Heatpipe design no water cooling or fan required Can be used together with closed fluid cells such as SmallCell 8 Electrochemistry solutions Temperature controlled ECCell Electrochemistry with temperature control and transmission optics such as phase contrast, DIC or fluorescence together with AFM. The unique combination of temperature controlled electrochemical measurements with high-end optics makes the ECCell the ideal tool for combined studies of temperature and potential sensitive biomolecules. Works with transparent or non-transparent substrates Accommodates standard coverslips, metal or silicon chips or other substrates Temperature range from ambient to 60 C 500 µl liquid volume with perfusion capability Sealed design for inert gas filling Wire electrodes and miniature reference electrode Suitable for conductive films or substrates (e.g., ITO coated glass) Compatible with common potentiostats Allows the use of high numerical aperture lenses for optimum fluorescence performance Unrestricted high resolution AFM imaging Heating Cooling Stage (HCS ) with electrochemistry cell Designed for electrochemistry AFM experiments with controlled heating and cooling of the sample. For opaque samples Temperature range from 0 C to 100 C Temperature resolution 0.1 C Twin stage thermoelectric element Drift minimized in all directions for full temperature ramps simultaneous to AFM imaging Heatpipe design no water cooling or fan required Top-view optical access for easy navigation 500 µl liquid volume with perfusion capability Sealed design for inert gas filling Wire electrodes and miniature reference electrode OEM Potentiostats from edaq JPK offers third party potentiostats for electrochemistry applications together with NanoWizard systems. edaq Pty Ldt. 14

15 9 Vibration and acoustic isolation Active vibration isolation from Accurion i4 Series compact table-top vibration isolation unit Active Workstation 780 and 900 active vibration isolation workstations Accurion GmbH Active vibration isolation from Table Stable TS 150 Small size table-top vibration isolation system for loads up to 150kg TS 140 medium size table-top system for loads up to 140kg Table Stable Ltd. Active air damping tables from TMC For large setups such as AFM/Raman combinations or confocal microscopy For less-critical vibration level environments JPK acoustic enclosure Approved for high performance applications: acoustic hood from JPK for utmost stability and isolation. Technical Manufacturing Corporation Divisible in two parts for easy transport and setup Special wall design made by sandwich construction and acoustic foam for best damping results Optional version without foam for S2 lab conditions 2 front doors with windows Dimensions: 1100 mm 1100 mm 1250 mm Demands table with 1100 mm 1100 mm area JPK base for acoustic enclosure Approved for high performance applications: base frame with top plate from JPK for utmost stability. Welded steel base frame 100 mm MDF sandwich table top Dimensions: footprint 1100 mm 1100 mm Height adjustable 15

16 10 Optics accessories TopViewOptics The optical system for non transparent samples or substrates such as HOPG, mica, ceramics, metall etc. For opaque samples Long working distance zoom lens from Navitar 2 mm µm field of view Köhler illumination, fiber light illumination Color CCD camera Easy positioning and focus adjustment TopViewOptics with boom stand The optical system for non transparent samples or substrates such as HOPG, mica, ceramics, implants etc. Can be used in combination with inverted microscopes. For opaque samples Long working distance zoom lens from Navitar 2 mm µm field of view Köhler illumination, fiber light illumination Color CCD camera Easy positioning and focus adjustment TopViewOptics for use with inverted optical microscopes or the JPK BioMAT The optical system for non transparent samples or substrates such as HOPG, mica, ceramics, implants etc. Can be used in combination with inverted microscopes. For opaque samples Long working distance zoom lens from Navitar 2 mm μm field of view Köhler illumination, fiber light illumination Color CCD camera Easy positioning and focus adjustment Fiber coupled detection module Allows the precise capture of light emitted by the SPM tip region into a fiber coupled detector. Suitable for home-build SNOM or other light emitting tip experiments. Fiber coupler for 50 µm core multi-mode optical fiber (FC connector) Fits to c-mount equipped inverted optical microscopes Precise manual xy positioning of the fiber end Built-in CCD-camera system for the alignment of emission spot to fiber center Three way optical switch for viewing sample, fiber end, and semi-transparent overlay Inverted optical microscope configurations Research lines of inverted optical microscopes from Zeiss, Olympus, Nikon, and Leica. Ask the JPK applications team for more information. 16

17 11 OEM solutions and small parts JPK sample holder for small samples Adaptor for standard sample holder. Holds magnetically fixed AFM metal stubs. JPK cable anchorage pillow Heavy weight pillow for fixation of cables to reduce noise coupling. JPK cantilever changing tool Probe loading station for convenient cantilever exchange. JPK biocompatible glue For cantilever or sample fixation, easy removable and biocompatible. JPK spare parts Available on request. Springs for different cantilever holders Fluid cell seals Adapters Coverslips Tubing for fluid cells 17

18 OEM syringe pumps JPK offers syringe pumps from several manufacturers. Aladdin AL-1000 from WPI Single pump for 1-60 ml or µl syringes Step motor with 400 steps per revolution and 5.1 cm/min (max speed)/ cm/hr (min speed) Pumping rate 1699 ml/hr with 60 ml syringe/0.73 µl/hr with 1 ml syringe KDS200 from KD Scientific Two-syringe pump for sizes 10 µl to 140 ml µm (1/16 step) advance per microstep Accuracy ± < 1% JPK 8x FluidicsModule JPK FluidicsModule with up to 8 different liquids such as buffer solutions Software-controlled flow rate Automated incubation or adding of chemicals Comprehensive safety package AFM cantilevers, substrates and standards The NanoWizard AFM platform is compatible with all commercially available cantilevers. JPK provides cantilevers from leading suppliers like AppNano, Budgetsensors, Mikromasch, NanoSensors, Nanotools, Nanocraft, NanoWorld, Team Nanotec, etc. AFM Cantilevers Standard contact and AC mode cantilevers High-resolution EBD tip cantilevers Chemically functionalized cantilevers Coated cantilevers Tipless cantilevers for customized force experiments Test grids and substrates And many more options Please visit our online store at cantilevers.jpk.com for an overview. NanoWorld AG, nanotools GmbH 18

19 CCD cameras The DirectOverlay optical integration can be used with any camera. The following CCD cameras are preferred and can be offered directly by JPK. Andor Technology ixon+ EMCCD Luca EMCCD Clara Interline CCD Andor Technology plc. Jenoptik ProgRes cameras series, such as MFcool CFcool Jenoptik AG The Imaging Source FireWire CCD color camera Imaging Source Europe GmbH IDS Imaging Development Systems Compact USB CCD and CMOS cameras of type µeye IDS Imaging Development Systems GmbH OEM Scanning Thermal AFM from Anasys JPK offers third party solutions for scanning thermal applications together with NanoWizard systems. OEM Indentation solutions JPK offers different third party solutions for indentation. OEM incubators for Live Cell experiments under controlled environments JPK offers different incubator models for LIVE CELL applications together with NanoWizard or CellHesion systems. OEM glove box system for NanoWizard AFMs The AFM system can be used in a glove box under controlled atmosphere. GS Glovebox Systemtechnik GmbH Please see dedicated brochures or product notes for more detailed information on our website at 19

20 Be more than a customer be our partner PK WORLDWIDE Providing the best service and support worldwide is an Our representatives in different countries are hand-selected essential part of JPK s philosophy. To accelerate this, JPK to offer a service which meets the JPK quality standards. We founded global offices in Singapore, Tokyo, Paris and Cambridge achieve this through long-term partnership and by continuous (UK). JPK has the largest applications and service team training of applications scientists and service engineers. For specialized in life science and nano science applications to more detailed questions our JPK applications team is just an support your research. or phone call away from you. JPK WORLDWIDE JPK WORLDWIDE JPK offers regular user trainings and courses for beginners or experts in our imaging facilities worldwide. Here users can learn to operate new instruments or get tips and tricks ranging from experimental procedures to sample preparation. BERLIN HEADQUARTERS UK FRANCE JAPAN SINGAPORE To find a representative in your region visit our website or call us. The handbook reflects a large variety of options and will grow further with latest developments. If you cannot find an accessory or operating mode in this handbook ask us directly. JPK is able to deliver customized solutions even for advanced applications. NanoWizard, CellHesion, TAO, BioMAT, NanoTracker, ForceRobot, Vortis, DirectOverlay, QI, HyperDrive, RampDesigner and ExperimentPlanner are trademarks or registered trademarks of JPK Instruments AG. All information and specifications are subject to change without notice. All rights reserved. 2012, JPK Instruments AG JPK Instruments AG Bouchéstraße Berlin, Germany tel.: fax.: Visit the JPK web site for more information and sign up for our enewsletter or follow us on Facebook or YouTube.