Introducing the Agilent 7000A Triple Quadrupole GC/MS

Transcription

1 Introducing the Agilent 7000A Triple Quadrupole GC/MS Paul Zavitsanos, Joe Weitzel, Monty Benefiel and Terry Sheehan May 2008 Page 1

2 What s a Triple Quad? Page 2

3 GC/MS Triple Quad (QQQ) Collision Gas (N2 ) Ion Source Ionize Quad 1 Hexapole Collision Cell Quad 2 Detector Page 3

4 What is MRM MS/MS? Multiple Reaction Monitoring EI: many ions from the source EI-MS/MS Product 2 Q1 SIM isolate precursor before CID Product 3 Product 1 CID + Q3 filter Page 4

5 Why MS/MS? Greater Selectivity Than SIM in complex samples EI-SIM selectivity proportional to spectral resolution no selectivity against ions with same m/z interference analyte EI-MS/MS Precursor selectivity same as SIM High probability that at least one product ions will be a unique dissociation product of the precursor BUT not the interference Product 2 unit mass resolution Product 3 Product 1 interference Precursor Ion The precursor ion should NOT be used for ion ratios or quantitation since the interferences will be the same as the SIM ion Page 5

6 Why a GC/QQQ System? Allows for the selective quantitation of target compounds in high chemical background samples Better S/N in complex matrices than can be achieved by single quad approaches Newer regulations in some markets specify analytical power commensurate with GC/QQQ Page 6

7 Why a Triple? A Picture is worth a thousand words. 100 fg HCB GC/MS Single Quad SIM MS SIM MS/MS MRM GC/MS/MS QQQ MRM EI 100fg HCB in DIRTY Matrix S/N: 116:1 RMS Page 7

8 SIM vs MRM for HCB 100 fg HCB in Clean Matrix 300 fg HCB in Diesel Single MS: SIM S/N=26:1 RMS S/N=6:1 RMS MS/MS: 283.8:213.9 S/N=37:1 RMS S/N=86:1 RMS Page 8

9 Technology Agilent took the Best technologies from their industry leading 597X Series GC/MSD: Heated monolithic gold plated quartz quadrupole Proven reliable high performance source design AUTOTUNE And the Agilent 6410A LC/QQQ: Linear acceleration enhanced Collision Cell Wide Mass-Bandwidth QQQ ion optics Mass Hunter software Page 9

10 Why Proprietary Heated Quartz Gold Quads? Heated monolithic gold plated hyperbolic quartz quadrupole is the gold standard in quadrupole instruments for GC/MS High 200C temperature quadrupole operation results in greater reliability with dirty samples Virtually eliminates the need to clean quads as a maintenance issue More stable tunes and methods over a longer period of time in real world sample environments High speed MRMs (500 MRMs/sec) possible with Agilent Quad electronics AUTOTUNE Friendly!!! Page 10

11 Agilent 7000A Analyzer Page 11

12 Analyzer Details Page 12

13 High Performance Differentially Pumped Manifold N 2 (1 sccm) He (1-5 sccm) He or H 2 (1-8 sccm) CH 4 (0-2 sccm) P1 5-20E-5 Torr Source P2 8E-5 Torr Collision Cell Analyzer Rough Pump Edwards Split Flow Turbo Page 13

14 Analyzer Details Page 14

15 Analyzer Doors Open Page 15

16 Proven source performance Inert source design proven over thousands of systems in current use Stay-Clean design extends maintenance intervals with dirty samples Source tune parameters in the Autotune file Dual Filament design reduces maintenance intervals Page 16

17 EI Source Details Page 17

18 High Performance Collision Cell Design Linear acceleration design is optimized for high speed performance without ion ghosting or cross-talk MRM speed to 500 MRMs/sec allows determination of more compounds per ion group High sensitivity with wide mass bandwidth eliminates the need to tune on my your Compound for optimum sensitivity Helium seeded chemical noise reduction collision cell increases S/N. Page 18

19 Q1-Collision Cell Interface Page 19

20 Collision Cell Details (2 rods removed for clarity) Page 20

21 High performance triple off-axis detector Ultra low neutrals noise Long life and high linearity Gain calibration corrects tune file for detector age to allow repeatable long term method performance. Same design as used in the 5975 GC/MSD Page 21

22 Triple-Axis Detector hyperbolic quartz transmission quadrupole analyzer steering rod shield for secondary particles Offset Z high energy dynode Y ion beam X triple channel electron multiplier Page 22

23 Q2-Detector Interface Page 23

24 Autotune Proprietary program tunes the source, mass analyzer, and detector for (as applicable): Ion transmission Mass axis calibration Mass resolution Detector gain vs Voltage Autotune settings are saved with the method for repeatable method performance. Manual Tune override is available Page 24

25 Performance Page 25

26 Agilent 7000A 100 fg HCB Sensitivity x MRM ( > 248.9) 100fgHCB_newvial_methHCB_MRM_PW1p21p2 527N2DAC_600HeDAC_35VColE_1e7Gain_2040EMV_0Baseline_12.d Noise (RMS) = 1.99; SNR (6.387min) = :1 RMS S/N vs Spec of 100: Abundance vs. Acquisition Time (min) 1pg HCB Benchmark comparison Agilent GC/QQQ Competitor 1 >1000:1 100:1 Page 26

27 Agilent 7000A OFN Sensitivity x MRM ( > 241.0) 100fgOFN_MRM_N2DAC1160_He18p5pct_30VColE_1e7Gain_2410EMV_GaugeOff_02.d Noise (RMS) = 1.10; SNR (5.471min) = fg OFN in clean Matrix 272:241 Transition 186:1 RMS S/N Abundance vs. Acquisition Time (min) Page 27

28 Innovation that led to this level of GC/QQQ sensitivity Page 28

29 Helium Seeded Collision Cell 1 ml/min N2 Collision Gas He* + Q1 Ions In Collision Cell Ions Out He* + N 2 N He N A A +. + N 2 Page 29

30 Helium Seeded Collision Cell 1 ml/min N2 Collision Gas He* + Q1 Ions In Collision Cell Ions Out 2.5 ml/min He Buffer Gas He* + He 2He + Heat He* + N 2 N He Page 30

31 Effect of Helium Buffer Gas Tested with HCB S/N 341:1 Helium Buffer Gas OFF Page 31

32 Effect of Helium Buffer Gas tested with Hexachlorobenzene S/N 341:1 Helium Buffer Gas OFF Noise Comparison S/N 1294:1 Helium Buffer Gas ON Page 32

33 Software Page 33

34 Mass Hunter MS Workstation Software Modern software interpretation of the proven industry standard GC/MS Chemstation platform Single software Platform for all Agilent MS Systems LC/SQ, LC/QQQ, LC/TOF, LC/QTOF GC/SQ, GC/QQQ ICP/MS Page 34

35 GC/QQQ Software Modules Acquisition Analysis Excel Reporting Page 35

36 Instrument Control UI/navigation Page 36

37 QQQ Acquisition Method Editor Dialog Single window acquisition setup Page 37

38 Agilent G7000A GC/QQQ Outstanding sensitivity 100fg of OFN on column at 100:1 S/N RMS in MS/MS mode using AUTOTUNE parameters verified at customer installation amu Mass Range 500 MRM/sec Speed Reliable heated monolithic gold plated hyperbolic quadrupoles Differentially pumped vacuum system CI/NCI upgrade available New Helium seeded collision cell technology Agilent 7890 GC with Capillary Flow technology. Mass Hunter Software. Page 38