Possibilitats Instrumentació actual HPLC. Marisa Lleberia Account Manager 1220 Infinity 1260 Infinity 1290 Infinity

Transcription

1 Possibilitats Instrumentació actual HPLC. Marisa Lleberia Account Manager 1220 Infinity 1260 Infinity 1290 Infinity

2 The Agilent 1200 Infinity Series HPLC 1200 Infinity Series 1220 Infinity 1260 Infinity Prep- Scale LC 1290 Infinity Isocratic, manual Low-flow Low- Flow LC Cap.LC Analytical flow Binary Prep-flow Isocratic Binary Gradient, manual Nano-LC Chip-LC Binary-SFC Binary Quaternary Gradient, automated Quaternary Quaternary Bio-Inert Isocratic Page 2

3 Agilent 1200 Infinity Series HPLC Raising the Standard in HPLC UHPLC for EVERY Lab 1220 Infinity LC Integracromatográfique s ted 600 bar, 5ml/min 80 Hz Most affordable HPLC and UHPLC 1260/1290 Technology 1260 Infinity LC Modular 600 bar, 5ml/min 80 Hz 2x or 10x UV Sensitivity 100% HPLC Compatible HPLC Price UHPLC Capability 1290 Infinity LC Modular 1200 bar, 5ml/min 160 Hz 1st Universal LC by ISET Highest Performance HPLC Service Costs Page 3

4 Instrument Column Compatibility Column Type Particle HPLC 3 5um UHPLC < 2um (STM) UHPLC Superficial Porous Column Length / mm Short: Long: CoIumn I.D. / mm Max Pressure / bar Agilent Column type Various RRHD RRHD RRHT RRHD RRHD RRHT Poroshell Poroshell 1290B/Q 1200bar 1260B 600bar 2) 1260Q/1220G 600bar 1) 2) 1) Conventional LC 400bar 2) 2) 2) 2) 3) 3) best good accept. compr. Any column of any dimension and particle can be used on 1220/1260/1290 Infinity LC s 1200 Infinity Series matches UHPLC columns pressure rating: 1290 Infinity LC: 1200bar + lowest delay volume (DV) for optimum support of mm ID STM (RRHD) 1260/1220 Infinity LC: 600bar + std DV for optimum support of 3-4.6mm ID STM (RRHT) & SP (Poroshell) STM-short: sub-2µm particles, 30 50mm length STM-long: sub-2µm particles, mm length HPLC: 3-5 µm particles Page 4 1) Because of delay volume. 2) Because of delay volume and pressure. 3) Because of pressure.

5 The Agilent 1200 Infinity Series - Power range pressure Infinity LC All analytical columns Method Transfer Higher Resolution Faster Analysis More viscous solvents Lower temperatures Semi-prep Infinity Binary LC 1260 Infinity Quat./Iso LC 1220 Infinity LC flow Page 5

6 From HPLC to RRLC From HPLC to RRLC on the 1220 Infinity LC by Varying Particle Size and Column Length mau Flow rate: 1.2 ml/min at 40 C x 50, 1.8 µm, Tg = 3 min Resolution peak 6 = 1.09 Run time = 3.2 min 3 x 100, 1.8 µm, Tg = 6 min Resolution peak 6 = 1.7 Run time = 5.7 min x 100, 3.5 µm, Tg = 6 min Resolution peak 6 = 1.29 Run time = 5.5 min min 3 x 150, 5 µm, Tg = 9 min Resolution peak 6 = 1.23 Run time = 7.5 min

7 Increasing Flow Rate for Shorter Analysis Time with Improved Resolution on Agilent 1220 Infinity LC mau mau VWD1 A, Wavelength=254 nm (TEST1220\PART D) VWD1 A, Wavelength=254 nm (TEST1220\PART D) x50mm, 1.8µm column at 1.2mL/min at 315bar, 40 C, 80Hz Resolution for peak 6= 1.09 min 3x50mm, 1.8µm column at 2.4mL/min at 510bar, 50 C, 80Hz Resolution for peak 6 = 2.53 Increase flow rate and adapt gradient time and temperature min

8 1220 Infinity LC Isocratic and Gradient RRLC at most affordable Price Most affordable access to 1260 Infinity Quality & Performance 1220 Infinity LC Integracromatográfique s ted 600 bar, 5ml/min 80 Hz Most affordable HPLC and UHPLC 1260/1290 Technology Available flavors: 1220 Infinity manual isocratic system (upgraedable) 1220 Infinity manual binary gradient system (upgradeable) 1220 Infinity automated binary gradient system Productivity: 10x higher than conventional HPLC systems 600 bar up to 5 ml/min (10 ml/min at 200 bar) 80 Hz detector speed Technology and Quality: 1260 and 1290 Infinity inside! Degassing: Same Technology/Parts as 1290 Bin/1260 Quat degasser Pump: Same Technology/Parts as 1260 Quat Pump (2-channel) Autosampler: Same Technology/Parts as 1260 ALS (no cooling) VWD: Same Technology/Parts as 1260/1290 VWD Upgrade capabilities Page 8

9 1260 Quaternary LC System Enhanced Quaternary & Binary RRLC at Quaternary HPLC 1260 Infinity LC Modular 600 bar, 5ml/min 80 Hz 2x or 10x UV Sensitivity 100% HPLC Compatible HPLC Price UHPLC Capability Productivity: 10x higher than 1200/1100 Quaternary HPLC 600 bar up to 5 ml/min (10 ml/min at 200 bar) 80 Hz Detector Speed Sensitivity: * ) 60,000 systems sold! Up to 10x higher than 1200/1100 Quaternary HPLC 10x by new 1260 Infinity DAD with 6 cm flow cell (± 0.6 µau) 2x by new 1260 Infinity VWD and 1260 Infinity DAD with 1cm flow cell Technology: Leverage of 1290 Infinity LC innovations Pump: New, build-in degassing unit and next generation inlet valves Detectors: Next generation VWD and DAD-technology Price: Similar as 1200 Series Quat (400 bar, 20 Hz, ± 6 µau ) Page 9

10 1260 Infinity Bio-inert HPLC I 100% Bio-inert Metal-free sample flow path (instrument and column) Stainless steel free solvent flow path Widest ph range (1-13, short-term 14) 600bar power range From lowest pressure up to highest pressure STM columns Semi prep 10ml/min When is this instrument recommended?? Infinitely Better for Bio-molecule Analysis Page 10

11 1260 Infinity Bio-inert HPLC 600 bar (UHPLC) now also for fast or high resolution Bioseparations What s new compared to our standard 1260 Infinity Quaternary LC Pump titanium based, no corrosion Active Seal included. Autosampler metal-free Capillaries and connections metal-free Detector Flow Cell metal-free ph-range 1-13, shortterm ph 14 Advantatges: Wide ph range High salt tolerance Lowest surface activity Bio-Inertness, no passivation needed 11

12 Target Applications Biologic Characterization Reverse Phase: Separation of Intact Biologics/Variants Ion Exchange: Separation of Isoforms Size Exclusion: Aggregate Analysis Peptide Mapping: Post-translational modifications 12

13 1290 Infinity LC More Affordable Access to the World s Best UHPLC 1290 Infinity LC Modular 1200 bar, 5ml/min 160 Hz st Universal LC by ISET Highest Performance HPLC Service Costs UHPLC Productivity with HPLC Service Costs Service cost similar to standard HPLC, attractive upfront contracts Based on proven robustness 2x higher than 1100 Series at introduction Increased parts lifetime / longer maintenance intervalls new design-for-supportability technology for faster maintenance, diagnostics and repair Technology: 1200 bar system pressure for pump, autosampler and valves to match any sophisticated application (method development solution). Highest detector sensitivity on the market (0.6 µau/cm noise level) Lowest autosampler carry-over (<0.001 %, <10 ppm for UV and MS detection in combination with 1290 Infinity Flexible Cube) New high-capacity solution for highest throughput analyses Page 13

14 Bombas UHPLC 1290 Infinity Robustez y alta capacidad de disipación de calor prestaciones. Capilares ( ) Multi-Capa para un óptimo intercambio de calor entre los cabezales de las bombas. Nuevo material del pistón: SSiC* (Carburo de silicio sintético). Proporciona Mejor Conductividad térmica y menor fricción para menor desgaste. Permite una optima disipación de calor Mayor presión x flujo (1200bar at 2 ml/min) Alarga el tiempo de vida de los sellos/ intervalos de mantenimiento (>150L) Un solo sello para fase normal y reversa SSiC*: Material cerámico de alta dureza formado por Carburo de Silicio sintético. Volumen Embolada variable (patentado) menor desgaste y mejores

15 1290 Infinity LC Delay Volume and Mixing Noise - How to achieve lowest delay volume, lowest noise and highest RT precision? 1200 Series Solvent A 1200 Series Solvent B Passive Damper µl Passive Mixer 400 µl Very Good Noise ~ 800 µl 1290 Inifinity Solvent A 1290 Inifinity Solvent B Active Damping < 5µl Microfluidic Mixing + = 35µl 5 µl / 40µl DV: 80x / 20x lower Lowest Noise < 50% Page 15

16 1290 Infinity Binary Pump Amortiguación electrónica de pulsos Motor del Pistón OF OF N Motores independientes para cada pistón-> minimas oscilaciones en la linea de base Pasos de 300 picolitros HPLC Inifinity Chanel 1290 Inifinity A Chanel B UHPLC 1290 Page 16

17 Agilent Jet Weaver: Nuevo sistema de mezclado Mezclador de alta eficacia. PLACA ACERO INOXIDABLE CON MICROCANALES DE 35µl de volumen La mas alta eficiencia de mezcla con el mínimo volumen para un bajo nivel de ruido V=35 µl, para aplicaciones UV estándar. Tecnología: Tecnología Propietaria de Microfluidos Multi-capa. Bandas de difusión de acero con estructuras de (100x120µm) Page 17

18 1290 Infinity Compartimento de columnas termost. Siguiente nivel en facilidad de uso: New leak funnel New capillary guide New 2PS/6PT, 2PT/10PT and 9PT/8PS Quick- Change high pressure valves Page 18

19 1290 Infinity Diode Array Detector - How the optics work Mirror Grating Programmable slit Deuterium lamp Max-Light cartridge cell 1024 element diode-array

20 Max-Light Cartridge Cell - Optofluidic waveguides Non-coated fiber (fused silica) High Light Transmission due to Total-Internal Reflection (TIR) principle (~ 100 % Light efficiency) Optofluidic Waveguides Long path length Small cell volume High light transmission Highest S/N Benefits: Highest Sensitivity (S/N) with small cell volumes (dispersion effects) More reliable and robust peak integration (automated) due to nearly no Refractive Index and thermal effects (solvent temperature) Cartridge design for ease of use Page 20

21 1290 Infinity Diode Array Detector - Baseline robustness due to significant reduced RI effects 1200 RRLC System (no mixer, damper) Flow rate: 0.5 ml/min (5-95 % ACN) Temp: 80 C, Data rate: 40 Hz, 230 nm with Reference Signal (360 nm) 1290 Infinity DAD 1200 DAD SL Next Level Sensitivity and Productivity Automated integration at any condition 2 20x higher sens with 1 cm Cell 8 80x higher sens with 6 cm UHS Cell Page 21

22 11.4 x Higher Sensitivity Infinity DAD compare to 1200 Series DAD SL mau Columns: 150 x 4.6mm Zorbax SB C18, 5µm Sample: Anthracene: 835 pg/µl Mobile phase: A: Water, B: Acetonitrile Elution: isocratic 80 % B Injection volume: 5 µl Flow: 1.5 ml/min DAD: 251/4nm, Ref= 450/80nm, 2.5Hz, slit width 4nm Infinity DAD 1200 Series DAD SL 1290 DAD 60 mm 1200 DAD SL 10mm Height [mau] Noise [mau] Signal/ noise Sensitivity increase min 22

23 1290 Infinity Diode-Array Detector - Programmable slit Absorbance (mau) Noise level nm 1 nm 2 nm 4 nm 8 nm Programmable slit for easy optimization of sensitivity, linearity and spectral resolution Page 23

24 Instrument to Instrument Method Transferability - Impact of delay volume and mixing behavior What happens with a programmed gradient? response 90% Gradient slope/ Mixing behavior Programmed gradient step 1200 Series Binary Pump SL 1290 Infinity Binary Pump 10% Delay volume time 1200 Series 1290 Infinity Total delay volume of the system (sum of capillaries, mixer, cells, valves..) Page 24

25 NEW Intelligent System Emulation Technology (ISET) The Solution Page 25

26 Intelligent System emulation technology - How it works (1290 to 1200) mau Injection Same gradient conditions by moving the gradient and by emulating the gradient mixing behavior Programmed gradient 1290 gradient 1200 gradient min Page 26

27 Method Transferability: 1290 Infinity LC - System Emulation Technology Concept F A Emulated System A Select System to be emulated by a simple mouse click: Agilent 1100 Series Agilent 1200 Series LC Agilent 1260 Infinity LC Agilent 1220 Infinity LC Alliance Page 27

28 Method transfer, pesticide example 2.1x100 mm Zorbax Eclipse Plus, 1.8 µm column, flow: 0,8 ml/min (A) 1100 Quaternary 400 bar 1 2, , ,11, (B) 1290 Infinity 1 200bar (C) 1290 Infinity 1200 bar with ISET Page 28

29 Agilent s Analytical LC Portfolio Compatible and Scaleable across entire Series 1290 Infinity LC 1260/1220 Infinity LC Methods compatibility Run 1100/1200 Series Methods on 1260/1290 Modules Upgrade 1000/1100 Infinity LC 1100/1200 Series with 1260/1290 Infinity Page 29