The equipment used share any common features regardless of the! being measured. Electronic detection was not always available.
|
|
- Emil Barker
- 5 years ago
- Views:
Transcription
1 The equipment used share any common features regardless of the! being measured. Each will have a light source sample cell! selector We ll now look at various equipment types. Electronic detection was not always available. Early absorption methods were based on using the human eye as our. In some cases, this is still a reasonable approach. Color comparison This eye-ball method only requires that you compare your unknown to a series of standards. Depth Comparison This is an extension of the color comparison method. The depths of the solutions are adjusted until there is a match between the two. d S c = R d R c S
2 All of the methods have the same general components Source, Wavelength selector Sample cell, Detector Read-out The actual arrangement of the components will vary based on the method. Source & Sample! selector readout With emission methods, the sample is an integral portion of the source. It is used to produce the EM radiation that will be measured. Source! selector Sample Common arrangement for UV/Vis readout! Source Sample readout selector Common arrangement for IR source! selector sample This is an emission method. All three of these work together as our source and sample.! selector readout Each system will have: The proper arrangement of components to measure the phenomenon. Components designed to work together. Proper slits, lenses, controls,...
3 Cell materials UV quartz, fused silica Visible glass, plastic (UV cells can be used) IR KBr, NaCl crystals are most common material nm range silica glass plastic standard liquid cuvette liquid sandwiched between two NaCl plates for IR For a general purpose instrument, we need a way to produce a broad range of! with reasonably uniform intensity. We can seldom obtain uniform intensity but most instruments can account for this. sample cell for gases Lets review some of the more common sources. The tungsten lamp is similar to a normal light bulb.! range: nm Useful in visible and near IR range. D 2 lamp D 2 + electrical energy D 2 * D 2 + h"! range: nm
4 This source produces emission lines specific for the element used to construct the cathode. Similar to a hollow cathode lamp in its use, it produces spectral lines by RF excitation of a metal salt - used for more volatile on nonconducting materials. This source is used with atomic absorption and fluorescence methods. RF coil salt containing bulb We typically only want to look at a single wavelength at any given time. Only interested in a single!. Scan a range of!, in sequence. The goal of a wavelength selector is to only allow a specific! to reach our and any given time. We can t really obtain a single wavelength, regardless of the source. Line sources are subject to the Doppler effect which causes line broadening. Our slits allow a range of wavelengths to pass through.
5 ! selected 50% effective bandwidth Absorbance filters Interference filters light allowed to pass material 1 material 2 Colored glass plates are used to absorb the! that are of no interest. Thin coating of CaF 2 or MgF 2 d! MAX = 2 d n N where: d = thickness n = refractive index N = order
6 Bandwidth is a function of the exit slit width. Changing the position of the prism will change the! that will pass through the exit slit. n! = d(sin i + sin r)! r i b! i - incident angle of light beam r - reflective angle of light beam d distance between lines n - order of reflection! - wavelength
7 As with a prism, we still need the proper lenses and slits in order for a grating to work as a monochromator. OK, now we need a way of detecting any light that has made it though our system. The purpose of a is to convert our response into a measurable signal. The approach taken varies based on the type of light that is being used. cathode anode 90 V + -
8 anode dynode conversion dynode window A single electron is ejected at the conversion dynode. Subsequent dynodes are ~90V more positive which results in the e - being accelerated and ejecting additional electrons. top view Amplifications of are obtained. p region n region
9 thermistor or thermocouple focusing mirror mirror i wafer slit - + pneumatic chamber absorbing film flexible mirror All instruments can be expected to have:! Proper amplification to produce a measurable signal.! Signal processing to remove, average data, drive a readout, A/D conversion.! A readout - meter, digital meter, chart,...! It may have some numerical processing capability.
10 light source 4 - sample cell 2 - wavelength selector shutter 6 - readout W lamp Another view grating This type of instrument works with a single light path. One must account for variations in response and source output for each!. cuvette! selector It is best when working with single! methods and individual analytes.. DB in time. recombining mirror A DB in time instrument works by splitting the light at regular intervals using a chopper. source s Half of the light passes through your sample, the other through a reference (blank).! selector chopper r The ratio of the sample to reference is used to measure absorbance and account for other variations.
11 This approach will account for variations in and source response since it is the ratio that is measured. Noise spikes are also reduced by using a lock-in R amp that only measures a signal with the right frequency - based on our chopper. S While a double beam in time instrument can reduce much of our noise and make it possible to obtain entire spectra, there are still problems. The major one is that you can t look at anything that changes at a rate near or faster that the chopper rate. Double beam in space. beam splitter With a typical instrument - no kinetic studies are possible. With this approach, we simply split the beam into two identical paths. No chopper is needed so we can look at time dependent processes. Not as much noise reduction. It also requires two s that are closely matched. With current computer technology, some manufacturers offer single beam scanning instruments. You acquire a blank run which is stored. Subsequent runs can then be corrected based on the blank.
12 One problem with traditional scanning instruments is that it can take several minutes to acquire a complete scan. Your sample can decompose during that time. Volatile solvent can evaporate. Also, don t forget that we all hate to wait. photodiode array The photodiode array is able to measure a range of! at once. You typically have a trade off between resolution and! range. A resolution of 1 nm is possible An entire spectrum can be measured in less than one second. sample absorption light source excitation monochromator emission monochromator fluorescence
13 source moving mirror source sample monochromator beam splitter fixed mirror sample data processing A complex signal is produced by passing light through an interference filter and varying the path length. sample
14
Spectroscopy in the UV and Visible: Instrumentation. Spectroscopy in the UV and Visible: Instrumentation
Spectroscopy in the UV and Visible: Instrumentation Typical UV-VIS instrument 1 Source - Disperser Sample (Blank) Detector Readout Monitor the relative response of the sample signal to the blank Transmittance
More informationSpectrophotometer. An instrument used to make absorbance, transmittance or emission measurements is known as a spectrophotometer :
Spectrophotometer An instrument used to make absorbance, transmittance or emission measurements is known as a spectrophotometer : Spectrophotometer components Excitation sources Deuterium Lamp Tungsten
More informationBasic Components of Spectroscopic. Instrumentation
Basic Components of Spectroscopic Ahmad Aqel Ifseisi Assistant Professor of Analytical Chemistry College of Science, Department of Chemistry King Saud University P.O. Box 2455 Riyadh 11451 Saudi Arabia
More informationCHAPTER 7. Components of Optical Instruments
CHAPTER 7 Components of Optical Instruments From: Principles of Instrumental Analysis, 6 th Edition, Holler, Skoog and Crouch. CMY 383 Dr Tim Laurens NB Optical in this case refers not only to the visible
More informationComponents of Optical Instruments
Components of Optical Instruments General Design of Optical Instruments Sources of Radiation Wavelength Selectors (Filters, Monochromators, Interferometers) Sample Containers Radiation Transducers (Detectors)
More informationChemistry Instrumental Analysis Lecture 10. Chem 4631
Chemistry 4631 Instrumental Analysis Lecture 10 Types of Instrumentation Single beam Double beam in space Double beam in time Multichannel Speciality Types of Instrumentation Single beam Requires stable
More informationComponents of Optical Instruments. Chapter 7_III UV, Visible and IR Instruments
Components of Optical Instruments Chapter 7_III UV, Visible and IR Instruments 1 Grating Monochromators Principle of operation: Diffraction Diffraction sources: grooves on a reflecting surface Fabrication:
More informationSCCH 4: 211: 2015 SCCH
SCCH 211: Analytical Chemistry I Analytical Techniques Based on Optical Spectroscopy Atitaya Siripinyanond Office Room: C218B Email: atitaya.sir@mahidol.ac.th Course Details October 19 November 30 Topic
More informationUltraviolet Visible Infrared Instrumentation
Ultraviolet Visible Infrared Instrumentation Focus our attention on measurements in the UV-vis region of the EM spectrum Good instrumentation available Very widely used techniques Longstanding and proven
More informationUniversity of Wisconsin Chemistry 524 Spectroscopic Components *
University of Wisconsin Chemistry 524 Spectroscopic Components * In journal articles, presentations, and textbooks, chemical instruments are often represented as block diagrams. These block diagrams highlight
More informationDETECTORS Important characteristics: 1) Wavelength response 2) Quantum response how light is detected 3) Sensitivity 4) Frequency of response
DETECTORS Important characteristics: 1) Wavelength response 2) Quantum response how light is detected 3) Sensitivity 4) Frequency of response (response time) 5) Stability 6) Cost 7) convenience Photoelectric
More informationApplications of Steady-state Multichannel Spectroscopy in the Visible and NIR Spectral Region
Feature Article JY Division I nformation Optical Spectroscopy Applications of Steady-state Multichannel Spectroscopy in the Visible and NIR Spectral Region Raymond Pini, Salvatore Atzeni Abstract Multichannel
More informationComponents of Optical Instruments 1
Components of Optical Instruments 1 Optical phenomena used for spectroscopic methods: (1) absorption (2) fluorescence (3) phosphorescence (4) scattering (5) emission (6) chemiluminescence Spectroscopic
More informationChemistry 524--"Hour Exam"--Keiderling Mar. 19, pm SES
Chemistry 524--"Hour Exam"--Keiderling Mar. 19, 2013 -- 2-4 pm -- 170 SES Please answer all questions in the answer book provided. Calculators, rulers, pens and pencils permitted. No open books allowed.
More informationModern Instrumental Methods of Analysis Prof. Dr. J.R. Mudakavi Department of Chemical Engineering Indian Institute of Science, Bangalore
Modern Instrumental Methods of Analysis Prof. Dr. J.R. Mudakavi Department of Chemical Engineering Indian Institute of Science, Bangalore Module No. # 02 Lecture No. # 08 Ultraviolet and Visible Spectrophotometry
More informationEfficiency of an Ideal Solar Cell (Henry, C. H. J. Appl. Phys. 51, 4494) No absorption radiative recombination loss Thermalization loss Efficiencies of multi-band-gap Solar Cell (Henry, C. H. J. Appl.
More informationcombustion diagnostics
3. Instrumentation t ti for optical combustion diagnostics Equipment for combustion laser diagnostics 1) Laser/Laser system 2) Optics Lenses Polarizer Filters Mirrors Etc. 3) Detector CCD-camera Spectrometer
More informationAbsentee layer. A layer of dielectric material, transparent in the transmission region of
Glossary of Terms A Absentee layer. A layer of dielectric material, transparent in the transmission region of the filter, due to a phase thickness of 180. Absorption curve, absorption spectrum. The relative
More informationChemistry Instrumental Analysis Lecture 7. Chem 4631
Chemistry 4631 Instrumental Analysis Lecture 7 UV to IR Components of Optical Basic components of spectroscopic instruments: stable source of radiant energy transparent container to hold sample device
More informationThe University of Toledo R. Ellingson and M. Heben
focal length, f Spectral Measurement Using a Monochromator, Thermopile Detector, and Lock-In Amplifier September 18, 2012 The University of Toledo R. Ellingson and M. Heben Where are We, Where we are Going?
More informationMeasurement Method of High Absorbance (Low Transmittance) Samples by UH4150 INTRODUCTION
INTRODUCTION With UH4150, a detector can be selected depending on the analysis purpose. When analyzing a solid sample which doesn t contain any diffuse components, by selecting the direct light detector,
More informationCONFIGURING. Your Spectroscopy System For PEAK PERFORMANCE. A guide to selecting the best Spectrometers, Sources, and Detectors for your application
CONFIGURING Your Spectroscopy System For PEAK PERFORMANCE A guide to selecting the best Spectrometers, s, and s for your application Spectral Measurement System Spectral Measurement System Spectrograph
More informationComponents of Optical Instruments
Components of Optical Instruments Optical spectroscopic methods are based upon six phenomena: 1. Absorption 2. Fluorescence 3. Phosphorescence 4. Scattering 5. Emission 6. Chemiluminescence Although the
More informationUSB. Part No Wavelength range. Spectral bandwidth 5 nm 4 nm Optical system
Visible range spectrophotometers V-1100 and VR-2000 V-1100 MODEL WITH MANUAL WAVELENGTH SETTINGS AND AUTOMATIC BLANK. VR-2000 MODEL WITH AUTOMATIC WAVELENGTH SETTINGS AND BLANK. V-1100 Part no. 4120025
More informationSouthern African Large Telescope. RSS Throughput Test Plan
Southern African Large Telescope RSS Throughput Test Plan Kenneth Nordsieck University of Wisconsin Document Number: SALT-3160AP0005 Revision 1.0 27 June, 2006 Change History Rev Date Description 1.0 27
More information6 Experiment II: Law of Reflection
Lab 6: Microwaves 3 Suggested Reading Refer to the relevant chapters, 1 Introduction Refer to Appendix D for photos of the apparatus This lab allows you to test the laws of reflection, refraction and diffraction
More informationHuman Retina. Sharp Spot: Fovea Blind Spot: Optic Nerve
I am Watching YOU!! Human Retina Sharp Spot: Fovea Blind Spot: Optic Nerve Human Vision Optical Antennae: Rods & Cones Rods: Intensity Cones: Color Energy of Light 6 10 ev 10 ev 4 1 2eV 40eV KeV MeV Energy
More informationThe FTNIR Myths... Misinformation or Truth
The FTNIR Myths... Misinformation or Truth Recently we have heard from potential customers that they have been told that FTNIR instruments are inferior to dispersive or monochromator based NIR instruments.
More informationCHAPTER 9 POSITION SENSITIVE PHOTOMULTIPLIER TUBES
CHAPTER 9 POSITION SENSITIVE PHOTOMULTIPLIER TUBES The current multiplication mechanism offered by dynodes makes photomultiplier tubes ideal for low-light-level measurement. As explained earlier, there
More informationECEN. Spectroscopy. Lab 8. copy. constituents HOMEWORK PR. Figure. 1. Layout of. of the
ECEN 4606 Lab 8 Spectroscopy SUMMARY: ROBLEM 1: Pedrotti 3 12-10. In this lab, you will design, build and test an optical spectrum analyzer and use it for both absorption and emission spectroscopy. The
More informationPhotolithography II ( Part 2 )
1 Photolithography II ( Part 2 ) Chapter 14 : Semiconductor Manufacturing Technology by M. Quirk & J. Serda Saroj Kumar Patra, Department of Electronics and Telecommunication, Norwegian University of Science
More informationSpectroscopy Lab 2. Reading Your text books. Look under spectra, spectrometer, diffraction.
1 Spectroscopy Lab 2 Reading Your text books. Look under spectra, spectrometer, diffraction. Consult Sargent Welch Spectrum Charts on wall of lab. Note that only the most prominent wavelengths are displayed
More informationPeriod 3 Solutions: Electromagnetic Waves Radiant Energy II
Period 3 Solutions: Electromagnetic Waves Radiant Energy II 3.1 Applications of the Quantum Model of Radiant Energy 1) Photon Absorption and Emission 12/29/04 The diagrams below illustrate an atomic nucleus
More informationAP Chemistry Cell Phone Spectroscopy Lab Adopted from Alexander Scheeline Department of Chemistry University of Illinois at Urbana-Champaign
AP Chemistry Cell Phone Spectroscopy Lab Adopted from Alexander Scheeline Department of Chemistry University of Illinois at Urbana-Champaign Back Ground Electromagnetic radiation Electromagnetic radiation
More informationChapter 16 Light Waves and Color
Chapter 16 Light Waves and Color Lecture PowerPoint Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display. What causes color? What causes reflection? What causes color?
More informationCHEM Course Outline (Part 14) Absorption Spectroscopy update 2011 For an html version of 2005 notes, click here
CHEM 524 -- Course Outline (Part 14) Absorption Spectroscopy update 2011 For an html version of 2005 notes, click here IX. Absorption Spectroscopy UV-VIS-near IR (Read Chap. 13) the most widely used analytical
More informationLight, Lasers, and Holograms Teleclass Webinar!
Welcome to the Supercharged Science Light, Lasers, and Holograms Teleclass Webinar! You can fill out this worksheet as we go along to get the most out of time together, or you can use it as a review exercise
More informationDual-FL. World's Fastest Fluorometer. Measure absorbance spectra and fluorescence simultaneously FLUORESCENCE
Dual-FL World's Fastest Fluorometer Measure absorbance spectra and fluorescence simultaneously FLUORESCENCE 100 Times Faster Data Collection The only simultaneous absorbance and fluorescence system available
More informationScintillation Counters
PHY311/312 Detectors for Nuclear and Particle Physics Dr. C.N. Booth Scintillation Counters Unlike many other particle detectors, which exploit the ionisation produced by the passage of a charged particle,
More informationLEP Optical pumping
Related topics Spontaeous emission, induced emission, mean lifetime of a metastable state, relaxation, inversion, diode laser. Principle and task The visible light of a semiconductor diode laser is used
More informationChapter 17: Wave Optics. What is Light? The Models of Light 1/11/13
Chapter 17: Wave Optics Key Terms Wave model Ray model Diffraction Refraction Fringe spacing Diffraction grating Thin-film interference What is Light? Light is the chameleon of the physical world. Under
More information(A) 2f (B) 2 f (C) f ( D) 2 (E) 2
1. A small vibrating object S moves across the surface of a ripple tank producing the wave fronts shown above. The wave fronts move with speed v. The object is traveling in what direction and with what
More informationNd: YAG Laser Energy Levels 4 level laser Optical transitions from Ground to many upper levels Strong absorber in the yellow range None radiative to
Nd: YAG Lasers Dope Neodynmium (Nd) into material (~1%) Most common Yttrium Aluminum Garnet - YAG: Y 3 Al 5 O 12 Hard brittle but good heat flow for cooling Next common is Yttrium Lithium Fluoride: YLF
More informationSection 1: SPECTRAL PRODUCTS
Section 1: Optical Non-dispersive Wavelength Selection Filter Based Filter Filter Fundamentals Filter at an Incidence Angle Filters and Environmental Conditions Dispersive Instruments Grating and Polychromators
More informationBlack Body Radiation. References: P.A. Tipler, Modern Physics, pp (Worth Publishers, Inc., NY, 1978).
Black Body Radiation References: P.A. Tipler, Modern Physics, pp. 102-107 (Worth Publishers, Inc., NY, 1978). Read carefully the material in this reference or any other Modern Physics text. The goal of
More informationSystems & Accessories
Light Source Sample Chambers Stepping Motor Controller Detector Variable Wavelength Fiber Optics Modules Tunable Light Sources For Applications In: Analytical Chemistry Physics Life Sciences Engineering
More informationHigh frequency sounds, beyond the range of human hearing, are called ultrasound.
Mr Downie 2014 1 Sound Waves To produce a sound the particles in an object must vibrate. This means that sound can travel through solids, liquids and gases. Sound cannot travel through a vacuum as it contains
More informationPrinciples of Fluorescence Techniques 2014 Urbana-Champaign, Illinois April 7-10, 2014
Principles of Fluorescence Techniques 2014 Urbana-Champaign, Illinois April 7-10, 2014 Basic Instrumentation: David Jameson (some of these slides were prepared by Theodore Chip Hazlett and Joachim Müller)
More informationMeasurement of the Speed of Light in Air
(revised, 2/27/01) Measurement of the Speed of Light in Air Advanced Laboratory, Physics 407 University of Wisconsin Madison, WI 53706 Abstract The speed of light is determined from a time of flight measurement
More informationData sheet for TDS 10XX system THz Time Domain Spectrometer TDS 10XX
THz Time Domain Spectrometer TDS 10XX TDS10XX 16/02/2018 www.batop.de Page 1 of 11 Table of contents 0. The TDS10XX family... 3 1. Basic TDS system... 3 1.1 Option SHR - Sample Holder Reflection... 4 1.2
More information5. Scintillation counters
5. Scintillation counters to detect radiation by means of scintillation is among oldest methods of particle detection historical example: particle impinging on ZnS screen -> emission of light flash principle
More informationDavid Jameson 3/23/2015
Principles of Fluorescence Techniques 2015 Urbana-Champaign, Illinois April 6-9, 2015 Basic Instrumentation: David Jameson (some of these slides were prepared by Theodore Chip Hazlett and Joachim Müller)
More informationShimadzu RF-5301 Fluorimeter operation guide for students
Department of Chemistry Teaching Laboratories Shimadzu RF-5301 Fluorimeter operation guide for students General directions Detailed instructions for use of the fluorimeter may be given in the lab script,
More information5. Scintillation counters
5. Scintillation counters to detect radiation by means of scintillation is among oldest methods of particle detection particle impinging on ZnS screen -> emission of light flash principle of scintillation
More informationThe Weber Conference on Advanced Fluorescence Microscopy Techniques December 12-17, 2011 Buenos Aires
The Weber Conference on Advanced Fluorescence Microscopy Techniques December 12-17, 2011 Buenos Aires Basic Instrumentation: David Jameson (many of these slides were prepared by Theodore Chip Hazlett and
More informationCompact High Intensity Light Source
Compact High Intensity Light Source General When a broadband light source in the ultraviolet-visible-near infrared portion of the spectrum is required, an arc lamp has no peer. The intensity of an arc
More informationInterference [Hecht Ch. 9]
Interference [Hecht Ch. 9] Note: Read Ch. 3 & 7 E&M Waves and Superposition of Waves and Meet with TAs and/or Dr. Lai if necessary. General Consideration 1 2 Amplitude Splitting Interferometers If a lightwave
More informationOPTI510R: Photonics. Khanh Kieu College of Optical Sciences, University of Arizona Meinel building R.626
OPTI510R: Photonics Khanh Kieu College of Optical Sciences, University of Arizona kkieu@optics.arizona.edu Meinel building R.626 Photodetectors Introduction Most important characteristics Photodetector
More informationUV-Vis-NIR Spectrophotometer Quick Start Guide
UV-Vis-NIR Spectrophotometer Quick Start Guide The following instructions are provided as a Quick Start Guide for powering up, running measurements, and shutting down the Lambda 950 UV-Vis Spectrophotometer.
More informationDivision C Optics KEY Captains Exchange
Division C Optics KEY 2017-2018 Captains Exchange 1.) If a laser beam is reflected off a mirror lying on a table and bounces off a nearby wall at a 30 degree angle, what was the angle of incidence of the
More informationLithography. 3 rd. lecture: introduction. Prof. Yosi Shacham-Diamand. Fall 2004
Lithography 3 rd lecture: introduction Prof. Yosi Shacham-Diamand Fall 2004 1 List of content Fundamental principles Characteristics parameters Exposure systems 2 Fundamental principles Aerial Image Exposure
More informationHigh Performance Thin Film Optical Coatings Technical Reference Document 09/13. Coatings Capabilities. Heat Control - Hot Mirror Filters
Heat Control - Hot Mirror Filters A hot mirror is in essence a thin film coating applied to substrates in an effort to reflect infra-red radiation either as a means to harness the reflected wavelengths
More informationPreview of Period 2: Electromagnetic Waves Radiant Energy I
Preview of Period 2: Electromagnetic Waves Radiant Energy I 2.1 Energy Transmitted by Waves How can waves transmit energy? 2.2 Refraction of Radiant Energy What happens when a light beam travels through
More informationMeasuring optical filters
Measuring optical filters Application Note Author Don Anderson and Michelle Archard Agilent Technologies, Inc. Mulgrave, Victoria 3170, Australia Introduction Bandpass filters are used to isolate a narrow
More informationLambda X65 series. UV/Vis Simplified for your Lab. Gerlinde Wita October 2015
Lambda X65 series UV/Vis Simplified for your Lab Gerlinde Wita October 2015 KEY MESSAGES More than 50 years history of winning UV-Vis technology by... THE HEART OF LAMBDA IN A SMALLER AND INNOVATIVE PACKAGE
More informationAqualog. CDOM Measurements Made Easy PARTICLE CHARACTERIZATION ELEMENTAL ANALYSIS FLUORESCENCE GRATINGS & OEM SPECTROMETERS OPTICAL COMPONENTS RAMAN
Aqualog CDOM Measurements Made Easy ELEMENTAL ANALYSIS FLUORESCENCE GRATINGS & OEM SPECTROMETERS OPTICAL COMPONENTS PARTICLE CHARACTERIZATION RAMAN SPECTROSCOPIC ELLIPSOMETRY SPR IMAGING CDOM measurements
More informationUV / VIS Spectrophotometer EMCLAB Instruments GmbH
A Professional Manufacturer UV / VIS Spectrophotometer EMCLAB Instruments GmbH EMCLAB Instruments GmbH Bismarckstrasse 120 47057 Duisburg Germany Phone: +49 203 3064042 Fax: +49 203 3064044 E-mail: info@emc-lab.de
More informationmos-500 SPECTROPOLARIMETER Circular Dichroism Spectrometer Everything you expect from a spectropolarimeter
mos-5 SPECTROPOLARIMETER Circular Dichroism Spectrometer Everything you expect from a spectropolarimeter Speed and Sensitivity Stability Modularity Low running cost user-friendliness innovation and Performance
More informationVisible & UV/Vis Spectrophotometers
Visible & UV/Vis Spectrophotometers Accurate Precise Affordable S20 Series UV/Vis Spectrophotometers The Unico S20 Series Spectrophotometers are simple, flexible, easytouse and maximize value while providing
More informationOptical In-line Control of Web Coating Processes
AIMCAL Europe 2012 Peter Lamparter Web Coating Conference Carl Zeiss MicroImaging GmbH 11-13 June / Prague, Czech Republic Carl-Zeiss-Promenade 10 07745 Jena, Germany p.lamparter@zeiss.de +49 3641 642221
More informationApplications Information
Applications Information Window Materials % TRANSMISSION 100 90 80 70 60 50 40 30 20 10 UV Sapphire UV Quartz Pyrex & Glass 100 200 300 400 500 600 700 800 900 Wavelength (nm) Pyrex only In applications
More informationDavid Jameson 4/4/2018
Principles of Fluorescence Techniques 2016 Urbana-Champaign, Illinois April 3-6, 2018 Basic Fluorescence Principles IV: David Jameson Basic Instrumentation (some of these slides were prepared by Theodore
More informationLight, Lasers, and Holograms Teleclass Webinar!
Welcome to the Supercharged Science Light, Lasers, and Holograms Teleclass Webinar! You can fill out this worksheet as we go along to get the most out of time together, or you can use it as a review exercise
More informationForm 4: Integrated Science Notes TOPIC NATURAL AND ARTIFICIAL LIGHTING
Form 4: Integrated Science Notes TOPIC NATURAL AND ARTIFICIAL LIGHTING OBJECTIVES: 1. Define natural and artificial lighting. 2. Use of fluorescent and filament lamps. 3. Investigation of white light and
More informationNIR SPECTROSCOPY Instruments
What is needed to construct a NIR instrument? NIR SPECTROSCOPY Instruments Umeå 2006-04-10 Bo Karlberg light source dispersive unit (monochromator) detector (Fibres) (bsorbance/reflectance-standard) The
More informationThe only simultaneous absorbance and f uorescence system for water quality analysis! Aqualog
The only simultaneous absorbance and fluorescence system for water quality analysis! Aqualog CDOM measurements made easy. The only simultaneous absorbance and fluorescence system for water quality analysis!
More informationGCMS-3 GONIOSPECTROPHOTOMETER SYSTEM
MURAKAMI Color Research Laboratory 11-3 Kachidoki 3-Chome Chuo-Ku Tokyo 104 Japan Tel: +81 3 3532 3011 Fax: +81 3 3532 2056 GCMS-3 GONIOSPECTROPHOTOMETER SYSTEM GSP-1 Main System Overview The colour and
More information- Optics Design - Lens - Mirror - Window - Filter - Prism
- Optics Design - Lens - Mirror - Window - Filter - Prism Optics Design Camera lens Design Laser Line Beam Design Lithography lens Design F-theta lens Design Beam Expender Design Zoom Lens Design, etc.
More informationLSM 510 META in Chang Gung University
Content LSM 510 META in Chang ung University LSM 510 META 路 理 The features and applications of LSM 510 META 01-09 Introduction of the hardware 10-12 Fluorescence observation in conventional microscope
More informationExercise 8: Interference and diffraction
Physics 223 Name: Exercise 8: Interference and diffraction 1. In a two-slit Young s interference experiment, the aperture (the mask with the two slits) to screen distance is 2.0 m, and a red light of wavelength
More informationElectromagnetic waves (Option G)
Electromagnetic waves (Option G) 12.1 The nature of electromagnetic (EM) waves and light sources Assessment statements G.1.1 Outline the nature of EM radiation. G.1.2 Describe the different regions of
More informationABC Math Student Copy. N. May ABC Math Student Copy. Physics Week 13(Sem. 2) Name. Light Chapter Summary Cont d 2
Page 1 of 12 Physics Week 13(Sem. 2) Name Light Chapter Summary Cont d 2 Lens Abberation Lenses can have two types of abberation, spherical and chromic. Abberation occurs when the rays forming an image
More informationUSER MANUAL FOR VISIBLE SPECTROPHOTOMETER
USER MANUAL FOR VISIBLE SPECTROPHOTOMETER 1 Table of Contents 1. MAIN USAGES...3 2. WORKING ENVIRONMENT...3 3. MAIN TECHNICAL DATA AND SPECIFICATIONS...4 4. WORKING PRINCIPLE...5 5. OPTICAL PRINCIPLE...6
More informationLeica TCS SP8 Quick Start Guide
Leica TCS SP8 Quick Start Guide Leica TCS SP8 System Overview Start-Up Procedure 1. Turn on the CTR Control Box, Fluorescent Light for the microscope stand. 2. Turn on the Scanner Power (1) on the front
More informationDoppler-Free Spetroscopy of Rubidium
Doppler-Free Spetroscopy of Rubidium Pranjal Vachaspati, Sabrina Pasterski MIT Department of Physics (Dated: April 17, 2013) We present a technique for spectroscopy of rubidium that eliminates doppler
More informationTHROUGHPUT OF AN OPTICAL INSTRUMENT CHEM 314
THROUGHPUT OF AN OPTICAL INSTRUMENT CHEM 314 OBJECTIVES Calculate the number of photons present in a single beam UV- Vis: At the source Entering the monochromator Incident on the diffracgon gragng Emerging
More informationevent physics experiments
Comparison between large area PMTs at cryogenic temperature for neutrino and rare Andrea Falcone University of Pavia INFN Pavia event physics experiments Rare event physics experiment Various detectors
More informationUses of Electromagnetic Waves
Uses of Electromagnetic Waves 1 of 42 Boardworks Ltd 2016 Uses of Electromagnetic Waves 2 of 42 Boardworks Ltd 2016 What are radio waves? 3 of 42 Boardworks Ltd 2016 The broadcast of every radio and television
More informationTRAINING MANUAL. Multiphoton Microscopy LSM 510 META-NLO
TRAINING MANUAL Multiphoton Microscopy LSM 510 META-NLO September 2010 Multiphoton Microscopy Training Manual Multiphoton microscopy is only available on the LSM 510 META-NLO system. This system is equipped
More informationThe Photoelectric Effect
The Photoelectric Effect 1 The Photoelectric Effect Overview: The photoelectric effect is the light-induced emission of electrons from an object, in this case from a metal electrode inside a vacuum tube.
More informationinstruments Solar Physics course lecture 3 May 4, 2010 Frans Snik BBL 415 (710)
Solar Physics course lecture 3 May 4, 2010 Frans Snik BBL 415 (710) f.snik@astro.uu.nl www.astro.uu.nl/~snik info from photons spatial (x,y) temporal (t) spectral (λ) polarization ( ) usually photon starved
More informationSkoog Chapter 1 Introduction
Skoog Chapter 1 Introduction Basics of Instrumental Analysis Properties Employed in Instrumental Methods Numerical Criteria Figures of Merit Skip the following chapters Chapter 2 Electrical Components
More informationPHYS 241 FINAL EXAM December 11, 2006
1. (5 points) Light of wavelength λ is normally incident on a diffraction grating, G. On the screen S, the central line is at P and the first order line is at Q, as shown. The distance between adjacent
More informationUVS-2800 Spectro UV-VIS Split Beam (PC) is a precise scanning
Spectro UV-VIS Split Beam (PC) is a precise scanning Spectrophotometer with a new design of 8 microprocessor automatic 2 row cell holder that moves noiseless with a special membrane. This Split Beam Spectro
More informationMotorized Axio Observer Start-up instructions
Start-up instructions 1. If using fluorescence turn on Fluorescent light source. TL light Source (Hal 100) 2. Turn on microscope using switch on lower left side of the microscope. 3. If imaging, turn on
More informationHR2000+ Spectrometer. User-Configured for Flexibility. now with. Spectrometers
Spectrometers HR2000+ Spectrometer User-Configured for Flexibility HR2000+ One of our most popular items, the HR2000+ Spectrometer features a high-resolution optical bench, a powerful 2-MHz analog-to-digital
More informationCOMPONENTS OF OPTICAL INSTRUMENTS. Chapter 7 UV, Visible and IR Instruments
COMPONENTS OF OPTICAL INSTRUMENTS Chapter 7 UV, Visible and IR Instruments 1 Topics A. GENERAL DESIGNS B. SOURCES C. WAVELENGTH SELECTORS D. SAMPLE CONTAINERS E. RADIATION TRANSDUCERS F. SIGNAL PROCESSORS
More informationCOMPONENTS OF OPTICAL INSTRUMENTS. Topics
COMPONENTS OF OPTICAL INSTRUMENTS Chapter 7 UV, Visible and IR Instruments Topics A. GENERAL DESIGNS B. SOURCES C. WAVELENGTH SELECTORS D. SAMPLE CONTAINERS E. RADIATION TRANSDUCERS F. SIGNAL PROCESSORS
More informationChapter 14. Tunable Dye Lasers. Presented by. Mokter Mahmud Chowdhury ID no.:
Chapter 14 Tunable Dye Lasers Presented by Mokter Mahmud Chowdhury ID no.:0412062246 1 Tunable Dye Lasers: - In a dye laser the active lasing medium is an organic dye dissolved in a solvent such as alcohol.
More informationCroma Enterprise Cromtech India
UV 1601 - Split Beam & Dual (equivalent to Double beam Spectro Photometer ) Wide wavelength range, satisfying requirements of various fields. The split-beam ratio monitoring system provides accurate measurements
More information