Double Emulsion Chip (100 μm etch depth), water-oil-water Part No
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1 Unit 1, Anglian Business Park, Orchard Road, Royston, Hertfordshire, SG8 5TW, UK T: +44 (0) F: +44 (0) E: W: Dolomite Microfluidics North America Office Blacktrace Inc, 29 Albion Place Charlestown, MA 02129, USA C: T: F: E: Double Emulsion Chip (100 μm etch depth), water-oil-water Part No product datasheet page Description 2 Benefits 2 Specifications 3 Sealing Interface 3 Geometry 4 Surface Coatings 4 Droplet Formation 5 Custom Options 5 Optical Transmission 6 The Dolomite Centre Limited Page 1 of 7
2 Description Featuring both a fluorophilic capillary and hydrophilic flow focussing droplet junction, this glass microfluidic chip enables the formation of water-in-oil-in-water droplets ranging from 80 to 150μm (outer) and 50 to 140μm (inner) in diameter. Applications include drug delivery, high throughput screening, food based emulsions and bio-analytical studies. The Double Emulsion Chip (100μm etch depth), W/O/W is compatible with the Linear Connector 7-way (Part No ) and the H Interface 7-way (11.25mm) (Part No ) for quick and reliable fluidic connection from tubing to chip. Left: Double Emulsion Chip (100 μm etch depth), W/O/W (Part No ) Top Right: Double emulsion of water-in-oil-in-water formed in Double Emulsion Chip (100 μm etch depth), W/O/W (Part No ) Bottom Left: Microscope image of double emulsion droplets with an outer diameter of 100μm Benefits Features a fluorophilic capillary and hydrophilic flow focusing droplet junction Rapid generation of water-in-oil-in-water droplets in the size range of 80 to 150μm (outer droplet) and 50 to 140μm (inner droplet) in diameter Ideal for producing and analysing micro-emulsions Excellent chemical compatibility High visibility (excellent access for optics) Compatible with Dolomite Multiflux Linear Connectors 7-way for quick and reliable fluidic connection MAR V.A.9 Page 2 of 7
3 Specifications Specification Channel cross-section at junction (depth x width) Wide channel cross-section (depth x width) Droplet size range Capillary Surface Chip Surface Double Emulsion Chip (100μm etch depth), W/O/W (Part No ) 100μm x 105μm 100μm x 300μm Outer droplet: Ø μm Inner droplet: Ø μm Fluorophilic Hydrophilic Number of inputs 5 Number of outputs 1 Chip size (length x width x thickness) Chip top and base layer thickness Channel length after junction Surface roughness of channels (Ra) Max. operating pressure Material Fabrication 11.25mm x 15.0mm x 4mm 2.0mm 6mm 5nm up to 30bar Glass (chip), Fused Silica (capillary) HF etching and thermal bonding Sealing Interface Connector body (PEEK) Seal Glass microfluidic chip Connector tubing PTFE, FEP Microchannel MAR V.A.9 Page 3 of 7
4 Geometry Channel at junction Wide channel 100µm 100µm 105µm 300µm Surface Coatings The glass channel surface is naturally hydrophilic. Fluorophilic coating has been applied to the fused silica capillary. This enables a sheath flow of water-in-oil to be formed, which allow droplets of water-in-oil-in-water to be created at the junction. Fluorophilically coated capillary Hydrophilic chip surface Water-in-Oil-in-Water Droplets Capillary ends at this point, enabling a sheathed droplet to be created at the junction MAR V.A.9 Page 4 of 7
5 Droplet Formation The size, consistency, and production rate of droplet formation is a function of several physical parameters, including: Channel size Viscosity and surface tension of the various fluids Presence of surfactants Miscibility of the fluids Use of hydrophobic or hydrophilic coating on the channel walls Total flow rate and relative flow rate of each fluid Flow stability To accelerate development work in droplet microfluidics, Dolomite offers a range of modular micro droplet systems featuring Dolomite s industry leading microfluidic pumps, connectors and chips. The Droplet Advanced System is recommended for use with the small droplet chip for optimum results. This features the Mitos P-Pump, which provides stable, pulse-free flow for generation of droplets with extremely consistent diameters (monodisperse). Please contact Dolomite to configure a droplet system that fits your requirements. Mitos P-Pump, Flow Resistors and Droplet Chips featured in the Droplet Advanced System Custom Options Other chip configurations are available on request. If you would like to generate droplets of a different size, operate with challenging fluids or perform complex droplet functions, Dolomite can design the junction geometry required. The range of Dolomite services available covers all aspects of the development process from characterization of liquids for droplet generation to the design of commercial instruments in the field of droplet microfluidics. Please contact Dolomite to discuss your application. MAR V.A.9 Page 5 of 7
6 Transmittance, % The Dolomite Centre Ltd Optical Transmission Spectral Transmittance of 2mm glass layer Wavelength, nm Optical Transmission of Glass MAR V.A.9 Page 6 of 7
7 MAR V.A.9 Page 7 of 7
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