BioInstrumentation Laboratory
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1 BioInstrumentation Laboratory Ian Hunter Vienna, May BioInstrumentation Lab, Mechanical Engineering, MIT
2 - Robotic endoscopes - Needle-free drug delivery devices - Eye micro-surgery robots - High throughput fiber testing machines - High throughput drug screening machines - Single muscle cell testing instrumentation - Very high speed digital microscope - X-ray microscope - 10,000 channel DNA mutational spectrometer - Raman laser microscopes - Nano-calorimeters - Nano-Walker robot - Micro mass spectrometer - Micro FT/FO Raman spectrometers - Micro quantum coherent spectrometers - Continuous gas chromatograph - Scanning tunneling electron microscopes m mm mm nm pm
3 Nanowalker Wireless communication for real-time control LEDs for optical tracking and global positioning Piezo tube arm Heatsinks Piezo tube legs Adam Wahab Embedded controller and power supply Micro-gripper module may be swapped for various tools Ruby feet
4 Tasks Microfabrication/Assembly Pick and place Adhesive bonding Property Measurement Electrical Thermal Optical Speed of sound Mechanical strength Magnetic Two units measuring the speed of sound through a conducting polymer wire Large-format Measurement Scanning Tunneling Microscopy (STM) Atomic Force Microscopy (AFM) Magnetic Force Microscopy (MFM) Micro-assay monitoring
5 Example: Multi-agent cooperative testing Two units hold a sample of polymer wire, while two other units measure its electrical conductivity.
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9 Small Imaging Transform Spectrometer Design of a small imaging transform spectrometer with custom optics, mechanics and electronics Can obtain Fourier transform spectra of coherent (laser) and incoherent (white light) sources High resolution spectra (< 1 nm resolution) Can obtain high resolution spectra quickly using special mathematical techniques Ellen Chen Prototype V0.5 Interference Patterns White Light LED
10 Miniaturized Continuous Chromatography via System Identification Eli Paster, BioInstrumentation Lab
11 Determining Chromatograms via Stoichastic System Identification Sample 1. Prepare sample for chromatographic methods Sample Pertubations Thermal Pertubations Flow Pertubations Multiple Columns 2. Perform stochastic or binary stochastic modulation of system input parameters TCD FID 3. Continuously measure the input and output signals Stoichastic System ID 4. Determine the impulse response (chromatogram) via stochastic system ID Constituent Chemical Identification and Monitoring 5. Compute relative concentrations (areas under the curve) and retention times, which are comparable to traditional chromatogram
12 Signal (arb) Signal (arb) Signal (arb) Signal (arb) Signal (arb) x System Identification Method Time (min) Output lags Cross-correlation Deconvolve Impulse Response Autocorrelation Impulse Response Input Time (s) lags Time (min)
13 Impulse Response Impulse Response Chromatogram Comparisons Traditional Chromatogram Heptane (125 o C) Similar retention times System ID Chromatogram 10 times longer data Improved peak shape Noisier baseline Impulse Response Impulse Response Time (s) Time (s)
14 Brian Hemond μms
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17 Detector Current [na] μms Miniature Portable Mass Spectrometer N H 2 O O N 14 N Ar Ar 2+ CO m/z
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24 μms Sensors MS Electric field voltage, 16 bit, 1 khz Electric field current, 16 bit, 1 khz Electron source voltage, 16 bit, 1 khz Electron source current, 16 bit, 1 khz Electron multiplier voltage, 16 bit, 1 khz Source slit width, 16 bit, 1 khz Analyzer slit width, 16 bit, 1 khz Magnetic flux density, 16 bit, 1 khz Inlet pump mass flow rate, 14 bit, 100 Hz Vacuum level, 16 bit, 100 Hz Internal temperature, 14 bit, 2 Hz Device GPS 3-axis position, 4 Hz 3-axis acceleration, 16-bit, 1 khz 3-axis angular velocity, 16-bit, 1 khz 3 axis magnetic heading, 12-bit, 100 Hz External Microphone, 16 bit, 20 khz Temperature, 14 bit, 2 Hz Relative humidity, 12 bit, 2 Hz Barometric pressure, 16 bit, 100 Hz Airflow, 16 bit, 1 khz Ambient illumination, 16 bit, 1 khz
25 Control and Orthogonal Stochastic Modulation Ion acceleration field voltage Electrostatic lens voltages Magnetic field flux density Electron source intensity Inlet pump mass flow rate Source slit width Analyzer slit width Temperature Electron multiplier voltage
26 End
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