Droplet Pillar Merger Chip
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1 Unit 1, Anglian Business Park, Orchard Road, Royston, Hertfordshire, SG8 5TW, UK T: +44 (0) F: +44 (0) E: W: Dolomite Microfluidics North America Office Blacktrace Inc, 29 Albion Place Charlestown, MA 02129, USA C: T: F: E: Droplet Pillar Merger Chip product datasheet page Description 2 Benefits 3 Specifications 4 Droplet formation 5 Surface coating 6 Geometry 6 Optical Transmission 7 IP License 7 The Dolomite Centre Limited Page 1 of 8
2 Part name Part number Droplet Pillar Merger Chip (200µm etch depth) Droplet Pillar Merger Chip (200µm etch depth), hydrophobic Droplet Pillar Merger Chip (200µm etch depth), fluorophilic The standard hydrophobic coating is not optimised for use with fluorocarbon oils. If fluorocarbon oils are to be used we suggest using a fluorophilic coating on the channel surface. Description The Droplet Pillar Merger Chip (200µm etch depth) is a glass microfluidic device designed for continuous merging in a controlled manner of two droplets with different composition in the volume range 50 to 175nl. This microfluidic chip features an in-line merging chamber mechanism allowing two-at-atime droplet merging between reference library samples and the test. The resulting merged droplets are stable and can be collected off-chip for further manipulations or analysis. The chip is indispensable for high throughput screening applications such as cell assays, ratiometric screening for protein crystallization and directed evolution of enzymes benefiting various industries including synthetic chemistry, fundamental biology and pharmacology. Top Left: Droplet Pillar Merger Chip (200µm etch depth) (Part No ) Top Right: H Interface 7-way (Part No ) with two Linear Connector s7-way microchannel (Part No ) Bottom: Merging mechanism on the Droplet Pillar Merger Chip (200µm etch depth), fluorophilic (Part No ) MAR va.11 Page 2 of 8
3 The Droplet Pillar Merger Chip (200µm etch depth) can be used with any microfluidic system setup merging from a pre-created droplet library. It has been designed to fit into the H Interface 7-way (11.25mm) (Part No ) with two Linear Connectors 7-way microchannel (Part No ). This connector uses micro alignment pins to ensure accurate alignment of tubing to channel. The Droplet Pillar Merger Chip (200µm etch depth) is ideal for integration with Dolomite s Mitos Dropix System Mode 2 for in-line droplet merging. In this configuration, this dropleton-demand system allows mixing or concentration gradients from 2 or more wells using the chip. Utilizing this droplet merging chip, ratiometric mixing from up to 24 wells, results in effectively infinite droplet combinatorial reactions. Mitos Dropix is a droplet-on-demand microfluidic system designed to generate droplets of up to 24 different samples and store up to 1000 droplets with sizes down to 50nl Benefits Innovative merging mechanism Ideal for integration with Mitos Dropix for in-line droplet merging One to one merging Wide temperature and pressure range Excellent chemical compatibility Quick connect/disconnect High visibility (excellent access for optics) 6 channels to merge 6 streams simultaneously, or increase the life of the chip MAR va.11 Page 3 of 8
4 Specification Specification Part No Part No Part No Channel cross-section at entrance and exit (depth x width) 200μm x 205μm Droplet size range (1 st droplet) nl Droplet size range (2nd droplet) nl Droplet size range (merged) nl* Maximum merging rate 10Hz Internal volume of chip Internal volume of a channel with merging chamber Surface coating Hydrophilic Hydrophobic Fluorophilic 3.9µl 0.6µl Number of inputs Number of outputs 1 (edge) 1 (edge) Number of channels with merging chambers 6 Chip size (length x width x thickness) Chip top and base layer thickness Surface roughness of channels (R a ) Max. operating pressure Material 11.25mm x 15.0mm x 4mm 2mm 5nm up to 30bar Glass Fabrication HF etching and thermal bonding * At a flow rate of 5 μl/min. At higher flow rates, the range of droplet volumes that can be efficiently merged varies from 50 to 125nl. MAR va.11 Page 4 of 8
5 Droplet Formation The pillar design enables the first droplet to be immobilised in mid-stream allowing the next droplet to merge. The merged droplet then exits the chamber. Please see diagram below: t = 0 s 30 ms 110 ms 250 ms 280 ms 320 ms 330 ms 370 ms 430 ms 450 ms 480 ms 490 ms 520 ms 550 ms 570 ms 590 ms 610 ms 630 ms Droplets travel left to right. Time lapse image sequence over a total period of 630 milliseconds, showing two consecutive droplets merging. A 30 nl sized red leading droplet mixes with a 40 nl sized trailing blue droplet resulting in a 70 nl sized purple droplet. Time stamps are not equally spaced, but instead capture significant instances during merging MAR va.11 Page 5 of 8
6 Surface Coatings The glass channel surface is naturally hydrophilic. This will form organic droplets in an aqueous carrier phase. To form aqueous droplets in an organic phase, a hydrophobic coating is required. Part numbers for the three chip types are shown below: Left: Aqueous droplets, in Organic carrier Droplet Pillar Merger Chip (200µm etch depth), hydrophobic (Part No ) Middle: Organic Droplets, in aqueous carrier Droplet Pillar Merger Chip (200µm etch depth) (Part No Right: Aqueous Droplets, in Fluorocarbon oil carrier Droplet Pillar Merger Chip (200µm etch depth), fluorophilic (Part No ) The hydrophobic coating is resistant to organic solvents. It can be removed using acidic or basic solutions, for example a 0.1M Sodium Hydroxide for 24 hours. The fluorophilic coating is resistant to organic solvents, strong acids, and bases. Geometry MAR va.11 Page 6 of 8
7 Transmittance, % The Dolomite Centre Ltd Optical Transmission Spectral Transmittance of 2mm glass layer Wavelength, nm Optical Transmission of Glass IP License Dolomite is a licensee of Japan Science and Technology Agency ( JST ) under JST s microdroplet generation technology. Mitos Dropix was developed by Dolomite under exclusive sub-licence with Drop-Tech Ltd. having won Dolomite's 2012 Productizing Science competition. Drop-Tech was formed from an academic collaboration between Cambridge University and Imperial College London and is the exclusive licensee of their patented droplet generation technology used in Mitos Dropix (Patent Pending: PCT/GB2013/051668). Please see our website for further details. MAR va.11 Page 7 of 8
8 MAR va.11 Page 8 of 8
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