H.S.K No. ITEM No. DESCRIPTION UNIT QUANTITY

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1 購買 ( 英文 ) 規格書 COMMODITY DESCRIPTION 관세분류번호 품목번호 품목및규격 단위 수량 H.S.K No. ITEM No. DESCRIPTION UNIT QUANTITY Spectral Confocal Laser Scanning Microscope with 8 channel imaging SET 1 A. Feature 1) The confocal laser scanning system is capable of spectral 8 channel imaging without crosstalk to capture snapshot of high-resolution morphology of complicated sample and slow moving live cell or phenomena. 2) The spectral confocal image is scanned point to point without impairing original resolution while removing efficiently crosstalk between chann This point to point scan shows best image compared to other scan type such as line-to-line scan and Nipkow disc scan. 3) Quality image is sequentialy taken with 8 channel imaging of 8 different fluorescent dyes. 4) This spectral detector consists of one PMT, motorized slit and prism optics. Motorized slit can choose arbitrary the range in spectrum out of fluorescence emission by prism. 5) This "Design-a-filter" can detect fluorescenc emission of any dyes, but filter-type confocal requires upgrade of new dedicated filter to that dye. 6) Emission range of each channel can be changed on-line without stopping scan, but filter-type confocal must stop scan to change detection filter. 7) More brighter image is obtained with this spectral detector compared to old filter-type confocal. Bright image with quality resolution resulted from 95% of high O.T.E (Optical Transmission Efficiency) of filter-free spectral detector. Compared to filter-type confocal, smaller intensity of laser line shows same brightness of confocal image. Less intensity of laser line save laser lifetime and prevent photobleach or photodamage of stained sample. 8) This tuneable bandwidth of spectral detector enable user to easily avoid crosstalk range in detection of the given dye. Even this tuneable bandwidth could separate signals of two dyes excited by one laser line sharing some crosstalk range. 9) Lambda Scan to produce emission graph by charting intensity vs. wavelength of given ROI. Easily investigate real emission spectrum of unknown dye or under various solution environment. Autofluorescence is easily confirmed and characterized for user to prevent bleedthrough. 10) BF detector can get non-confocal BF images for overall morphology and background for overlay with fluorescence channels. 11) AOTF (Acousto Optical Tunable Filter) technology to pinpoint excitation laser line. But old excitation filter of old filter-type confocal shows broad selection of laser lines and could not pinpoint each wavelength. AOTF attenuated intensity continously within several millisecond, 1

2 but filter-wheel of old confocal attenuate 8 step with several hundred millisecond 12) Sequential Scan of one laser line to respective channel resulting in general crosstalk imaging User can easily 8 channel images without considering general bleedthough between channels 13) Multi-dimensional scan mode : combination of X, Y, focal position(z), time(t), wavelength(lambda) and multi-site on stage 14) High Q.E. (Quantum Efficiency) of PMT from UV to IR : 30-40% Q.E. 15) User-switchable light weighed scanhead between upright and inverted microscopes. Scanner head fits onto upright and inverted microscope. 16) Compact desgin save workspace in user' lab : all laser tubes are contained by personal computer unit. 17) Super Z stage with 3nm step accuracy and 1.5mm travel range for high resolution 3D XYZ imaging and live XZ scan. 18) 4 diode laser tubes with less noise, high power, no alignment and no cooling. blue, green and red lasers But, many confocals have gas lasers which require regular laser alignment and need air cooling and stabilisation time. 19) ACS optics : no chromatic aberration of UV, VIS and IR image focussed on the same focal plane 20) digital microscope : one-touch click make microscope ready for each contrast method such as bright field, fluorescence, PH and DIC For each objective, last used settings such as light intensity, aperture, field diaphgram size and so on are automatically saved. 21) digital inverted microscope optimised for long-term obseravtion of slow moving live cell : no focus drift Even 3 day inclubation could not change focus of inverted microscope. 22) External fluorescence light source provide cold light for fluorescence with fast shutter and 5 step intensity control. This light source have the structure which do not requires lamp alignment after lamp exchange. 23) Simple design of confocal software : easy to use, easy to learn and easy to teach Image acquisition, image view, image processing and quantification are performed in this simple software. 24) One page acqusition interface enable user eaily configure and save specific acquisition settings of each sample. Acquired images have acqusition settings which can later be recalled to current setting. 25) Images by focal serial scan can be model into 3D by projection algorithms such as MIP (Maximum Intensity Projection), average intensity projection, transparency projection and color-coded projection. 26) 3D rotation of the reconstructed 3D model, timelapse and Z sweeping can be recorded into AVI movie files. 27) Image processing : changing bits, crop multidimensional data, image enhancement, image baseline, image colour adjustment segmentation, morphological filter and so on 28) Quantification such as line profile, histogram and stack profile. All quantification step provide ROI tool, graphing, chart, save to XML report and exporting raw data to excel files. 29) Colocalization anaysis display the colocalized pixels as several formats and shows scatter plot between two channgels. Analysis module calculate the degree of colocalisation, Pearson's correlationcoefficient and area percentage. 2

3 B. Specification 1. Scan head Scanning concept : optically correct scanning at low interia Spectral detector, pinhole, double dichroic beamsplitter, beam expander, mini scanner Short length of optical path, miniaturized scanner and beam expander Light weight to switch easily scan head between upright and inverted microscope Optical Transfer Efficiency (OTE) of 95% a) spectral detector with high quantum efficiency 1 PMT, motorized slits and prism for confocal fluorescence image 1 BF detector for non confocal bright field image Wavelength detection band adjustable during scanning Minimum spectral interval of 1nm and 2nm default High sensitive PMTs with 30-40% Q.E. one 12/16bit AD converters for PMT b) Pinhole one detection pinhole No need to align pinholes along with optical path Single, computer controlled, variable detector pinhole Precise localization of multi fluorochromes / no pixel shift illumination pinhole per each laser c) miniaturized scanner scanner with two independent galvanometers Cardanic single-mirror system / xy scanning simultaneously Scan resolution from 128x128 up to 2048 x 2048 pixels speed from 0.36 to 3 frames per second; 1 frame per second at 512 x 512 live XZ scan operates at 10Hz Electronic zoom from 1.0 to 15 times 3

4 2. Lasers and electronics a) Laser unit 405nm diode solid state laser, 50mW 488nm diode solid state laser, 15mW 532nm DPSS, 15mW 633nm diode laser, 15mW b) Laser coupling device with AOTFs AOTF for all lasers to pinpoint excitation laser line and to attenuate its intensity, 8 channels 3. Microscope part a) High resolution Z stage Z-displacement of sample is independent of optical microscope and allows for unimpeded use on both sides of microscope Z-Galvo stage : step accuracy = 3nm, Max. travel range = 1.5mm Galvanometric stage of extra high precision b) Safety lock To prevent the accidental exposure of laser light to user Safety lock will be shut down the light when illumination column of microscope move back. c) Transmitted Light Detector Widefield PMT to visualize non-confocal DIC or BF image d) High performance Advanced Digital Inverted Microscope Infinity-corrected ACS series objective and specially modified glassless phototube ACS optic : no shift between UV image and VIS-IR image, no chromatic aberration System developed newly and optical excellence meets superior versatility to allow all experiments At high magnification, all Apo objectives are configured It basically includes Auto contrast manager, Auto illumination and Stability manager which provide razor-sharp, brilliant images at clicking button Powerful integration, adapted but individual flexibility, intelligent imaging and good experiences. 4

5 1) Magnification : 100x to 630 in optical base 2) Field of view : 22mm 3) Universal stand In CTR4000 electronics box and information display 77x49cm Interpupillary distance adjustment : 55 to 75mm and inclined : 30 RS232 and ready for USB Co-axial and coarse focusing adjustment knob Field and aperture diaphragm : built-in type All of HCS (Harmonic Component System) Optic system 4) Revolving nosepiece Setuptuble holes and manual change between objective manual focus dials : coarse and fine adjustment of Z movement 6 x 25M standard, color coded 5) 7 freely programmable buttons with fixed functions Illumination manager, camera ports, subsequent magnification and fluorescence functions 6) Stage Manual 3 plate stage as ceramic coated, over 20 different inserts available Rectangular, traverse range 52x76mm It can be equipped with stage cooling and heating inserts 7) Illuminator for transmitted Halogen lamp 12V 100W and variable voltage within indication manul field diaphragm and manual stop 8) Condenser unit Turret for optical elements with 4 large opening for prisms, DF stop, BF, PH rings and 3 small for BF, PH rings Integrated motorized aperture diaphragm 5

6 Suitable for 1.25x to 100x Motorized condenser turret with coded Fixed condenser head and motorized lens for low magnification 9) Eyepiece 10x field of view index 20, pair 10) Fluorescence axis Motorized 6 filter slots, 4 fluorescences, 1 transmitted or Confocal and 1 spare cube slot Stopper with BG38 filter to prevent unnecessary exposure of UV onto specimen Fluorescence filters for microscope : A, I3 and N2.1 light color excitation dichroic emission Filter System A UV BP LP425 Filter System I3 blue BP LP515 Filter System N2.1 green BP LP590 11) Automation functions a) IM : Illumination Manager, adjustment of brightness, aperture and field diaphragms to objectives and contrast technique b) CM : Contrast Manager, adaptation of optical elements such as prism and right rings to objectives and contrast technique c) FIM : Fluorescence Intensity Manager, adaptation of brightness in 5 fixed levels d) IFW : Integrated Fast Filterwheel, very fast switching of excitation with changeover times of less than 0.05 second e) Adjustment of field diaphragm to eyepieces or camera chips(round or rectangular diaphragms) 12) Objectives ACS APO 10x/0.30 CS - /C1 K3 3.0 HC PL FLUOTAR 20x/0.50 ACS APO 40x/1.15 Oil CS 0.17 /E K ACS APO 63x/1.30 Oil CS 0.17 /E K ) EL6000 : External fluorescence light source 120W Hg lamp 2000hour lifetime cold fluorescence light, no heat to microscope alignment free after exchange of Hg lamp 6

7 fast 6ms shutter and 5 step attenuation 14) anti-vibration table for inverted microscope, granite base plate 4. Confocal software a) Configuration mode microscope settings : imaging port, LAS connection, objective details laser settings : iniitate laser line and intensity of multi-line laser dye database : user look into the information of several dyes including excitation spectra and emission spectra b) Acquire mode Predefined beampath settings Combinatorial scan mode of X, Y, Z, Time, wavelength Scan resolution from 128 x 128 to 2048 x 2048 Scan speed from 0.36 to 3 frame per second continuous detection pinhole size Electronic zoom from 1 to 15, zoom-in and undo zoom-in Series scan, Single scan, Preview scan button Accumulation and Frame average Upper boundary, Lower boundary and number of frames for Z series scan Start wavelength, End wavelength, Interval and number of frames for wavelength scan simple time lapse dialog c) Image Window Separate Channel Button to show on and off the given channels Double clicking on image to go into full image and revert into multichannel view Play button to show all images from serial scan such as Z scan, T scan and Lambda scan Software zoom button to zoom in/out the captured image progress control to navigate into the serial images Original > GlowOverUnder > BlackAndWhite button : to assess how pixel is saturated or to compare how bright between channels ROI tools for preview images : scale bar, rectangle, ellipsis Annoataion toos for taken images : scale bar, rectangle, ellipsis, count, distance, text box 7

8 Autocontrast tool and show histogram snapshot to adjust image quality Gallery view to serial data, MIP view for Z series data d) Process mode Image enhancement such as brightness, contrast and gamma baseline, color adjustment, segmentation, seeding Arithmetic operation between images or between image and constant Merging and Separation for editing serial images 1) 3D Visualisatioin mode 3D visualization for projection and movie making Maximum Intensity Projection Average Intensity Projection Transparency Projection Color coded projection Translate 3D model Rotate 3D model Zoom 3D model Movie maker for rotation of 3D model e) Quantification mode Select a certain geometrical shape as region of interest as line, rectangle, ellipse and polygon Graph or chart to shows the quantified values Intensity profile analysis along with lines Histogram analysis to given ROI or whole channels Stack profile analysis to measure intensity of given ROI or whole channel versus Z position or wavelength Report wizard to generate XML report of analysis results Axis scale can be set to auto or absolute mode in chart Export trace of graphed value into MS excel file Chart shows sum, mean, max, min of intensity, area and count 5. Computer system 8

9 MS Windows XP professional or higher Computer specification will be supplied the latest version if it is delivered C. Standard accessories Documentation for principle & operation instruction Instruction manual Immersion oil Laser protection cover D. Consisting of ; * Spectral Confocal Laser Scanning Microscope system 1) Leica TCS SPE w. DMI 4000 CSQ V-VIS 1 set 2) LAS AF SPE1000 3D Visualization 1 set 3) HC PL FL 20x/ /D ea 4) HCX PL APO 40x /1.25 Oil, PH3 CS,017 1 ea 5) Microscope Table Inverted passive 1 set 6) Insert for Super Z inverse, universal 1 ea 7) Filter system A 1 ea 8) SPE1000 TLD Kit Invers 1 set E. Remarks a) The warranty for this instrument is one year from the date of installation. b) Installation and operation training shall be performed by maker's certified / trained engineer with free of charge. c) The software developed within 5 years since the main instrument has installed should be provided in free of charge. 9

Leica TCS SP8 Quick Start Guide

Leica TCS SP8 Quick Start Guide Leica TCS SP8 System Overview Start-Up Procedure 1. Turn on the CTR Control Box, EL6000 fluorescent light source for the microscope stand. 2. Turn on the Scanner Power