Axio Zoom.V16 The Fluorescence Zoom Microscope for Large Fields
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1 Product Information Interactive PDF internet-link video/animation Release 1.0
2 It s About Brilliance. Because Only the Best Is Good Enough In Brief The Advantages The Applications In 1994, the molecular biologist Douglas Prasher discovered that GFP was suitable as a marker for proteins. At the time, hardly anybody suspected what this would mean for the development of the stereo microscope: stereo magnifiers experienced a renaissance. Scientists were now able to view complete model organisms in fluorescence contrast. Alongside the enthusiasm for these devices, the performance of stereo and zoom microscopes has also grown noticeably, with systems from Carl Zeiss keeping the bar high. As we say: they are simpler, more intelligent, more integrated. They also give your work the brilliance it deserves. The System Technology and Details Service Animation 2
3 : Simpler. More Intelligent. More Integrated. Bright Fluorescence in Large Fields For the first time, combines a 16x zoom with a high numerical aperture of NA 0.25, moving to the forefront of all known stereo and zoom microscopes. It achieves a very high aperture in the medium zoom range already: you get superior fluorescence brightness in large object fields. With Plan-NEOFLUAR Z 2.3x you can achieve a numerical aperture of NA 0.5 in an object field of 1.5 millimeters. Use ApoTome.2 for fluorescence imaging to obtain optical sections. You get a system that masters routine screening as well as your most demanding multidimensional imaging applications does it all brilliantly. Optimized Zoom for a Variety of Applications The ezoom of works with a motorized iris diaphragm coupled to the zoom. Simply select the best mode for your purpose by pressing a button on the SYCOP 3. Brightness mode: Observe fluorescence images over the complete zoom range with highest possible brightness. Eyepiece mode: This is ideal if you work mainly with ocular observation using conventional illumination. You can zoom from large object fields with maximum depth of field to high magnifications with maximum resolution. Camera mode: The optics of your adapt to the performance of your camera. You get an optimal relation between resolution and depth of field across the whole zoom range. Intelligent Transmitted Light Over the Whole Zoom Range In addition to brightfield, darkfield and oblique illumination, you can get an increased contrast brightfield at the touch of a button. With the Best Mode button, determines the actual optical state and optimizes transmitted light automatically. Use the Adjust control to fine-tune Best Mode more precisely to your application. Then simply save your setting and reload it for your next experiment - once again, at the touch of a button. 3
4 Your Insight Into the Technology Behind it At the Limits: Conventional Zoom Technology The zoom body is the core of stereo and zoom microscopes. When zooming, lenses have to be positioned precisely. Until now, a metal component milled with great care would determine the exactness of this movement, and with it the optical quality of your microscope. Now ezoom Images Are Twice as Sharp With, ezoom replaces the mechanical curve with an electronic one. Stepping motors position the moveable lenses precisely and take the tolerances of the individual lenses into account. Each zoom body describes its own zoom curve and captures visibly more details. ezoom follows the base line for image sharpness over the magnification range with a doubled precision, compared to a mechanical zoom body. Zoom curves can be programmed individually. Dectocus position in µm Magnification depth of field curve, within these parameters images are in focus typical defocus curve of a single zoom channel with mechanical zoom curve typical defocus curve of a single zoom channel with eletronic zoom curve When the micro clapper of the computer-controlled glue leveling machine brings ezoom s lens in the zoom body into position it is glued and cured with UV light. From around 7,000 reference points, the zoom body adjustment device calculates the zoom control curve. 4
5 Tailored Precisely to Your Applications Typical applications, typical specimens Plant biology Developmental biology Forensics Task Observe whole living plants Screen a large number of whole model organisms, e.g. embryos of Zebrafish and Drosophila, Identify promising specimens, Image with high fluorescence intensity Find minute human traces on large objects provides Fully motorized Fully motorized Plan-NEOFLUAR Z 1x SYCOP 3 SYCOP 3 HXP 200 C Plan-NEOFLUAR Z 2.3x Plan-NEOFLUAR Z 1x Filterset FS 38 HE HXP 200 C Filterset FS 38 HE, FS 43 HE HXP 200 C Filterset FS 27 3D - micromanipulator aureka ( Example Identify and document weakly autofluorescent gland tissue of Nepenthes, Isolate glands of interest Sort and evaluate Drosophila embryos, Acquire multidimensional images Identify human cells, Isolate and transport them to the PCR tube 5
6 Your Insight into the Technology Behind It ApoTome.2 Create Optical Sections of Your Fluorescent Samples With structured illumination, you know that only the focal plane appears in your image: ApoTome.2 recognizes the magnification and moves the appropriate grid into the beampath. The system then calculates your optical section from three images with different grid positions. It s a totally reliable way to prevent scattered out-of-focus light, even in your thicker specimens. You get images with high contrast in the best possible resolution simply brilliant optical sections. Animation from Mike Davidson, FSU, Tallahassee A B C D ApoTome.2 Grid in the Beampath Fluorescence excitation light passes through two glass plates in the ApoTome.2 slider. When a grid structure is applied to the first glass plate, the grid pattern is imprinted in the excitation light. A scanning mechanism tilts the second glass plate and the image of the grid is laterally shifted in the focal plane of the specimen. Schematic illustration of the grid projection. A-C: raw images with different positions of grid D: optical section through sample 6
7 : A Flexible Choice of Components for fluorescence screening 1 Microscope microscope body Binocular ergo phototube Z 0-30 mot with PL 16x/16 eyepieces Stand base 450 Focus motor 3 with profile column Objective nosepiece Z, 2x cod Mechanical stage S 150x100 mot; CAN System Operator Panel SYCOP 3 with Controller EMS 3 Option: ApoTome.2 module 2 Objectives Plan-NEOFLUAR Z 1.0x/0.25 FWD 56mm Plan-NEOFLUAR Z 2.3x/0.57 FWD 10.6mm Illumination Fluar Illuminator Z mot Reflector modules Z FL P&C (ACR or ACR RW) HXP 200 C (metal halide) Transillumination top 450 mot (brightfield, brightfield+, darkfield, oblique illumination) Cameras AxioCam HRm AxioCam MRm 5 Software Recommended ZEN modules: Multi Channel, Z Stack, Time Lapse (imaging) Tiles & Positions (imaging with scanning stage) 7
8 : A Flexible Choice of Components for fluorescence imaging 1 Microscope microscope body Binocular phototube Z 15 with PL 10x/23 eyepieces Transmitted light base 300 Coarse/fine drive with profile column 490mm Mount S with diameter 76mm 2 Objective Objective Plan-NEOFLUAR Z 1.0x/0.25 FWD 56mm 3 Illumination Fluar Illuminator Z mot Reflector modules Z FL P&C HXP 200 C (metal halide) Brightfield, darkfield, oblique transmitted light
9 : System Overview 9
10 : System Overview 10
11 : System Overview 11
12 Technical Specifications Technology and Details Objective PlanApo Z 0.5x Parfocal length 164 mm PlanApo Z 1.0x Parfocal length 164 mm Apo Z 1.5x Parfocal length 133 mm PlanNEOFLUAR Z 1.0x Parfocal length 133 mm FWD (mm) Eyepiece PL 10x/23 Magnification Object field (mm) x... 56x x x x x x 56 7x x Eyepiece PL 16x/16 Magnification Object field (mm) 5.6x... 90x x x x x x x
13 Count on Service in the True Sense of the Word Your results really matter to us: we want you to get the best you expect from your microscope. Depend on Carl Zeiss for everything you need: technology, software, advice and service. We stay with you long after installation of your microscope on site. ZEISS specialists will continue to maintain your systems, repair them, supply spare parts and much more. Just call us: we are always here for you. Total Protection with Your Carl Zeiss Service Contract It s the safe and practical way to preserve the efficiency of your microscope system. Our service contract protects you against expensive downtime. Preventive Maintenance Plus Optimizes Performance Our specialists will maintain and tune your system at regular intervals. You get valuable advice and comprehensive answers to any and all questions. We will also keep you right up to date on developments in your field of application. The Standard Contract Also Includes Repairs and Support In addition to all the services of Preventive Maintenance Plus, the standard contract covers all repair and support services. The only costs you will ever pay are for replacement components. Another important aspect of the standard contract is installation of software updates your system will always be running the latest program. The Premium Contract Covers Spare Parts Too Opt for the premium contract and you will have all services of the standard contract, plus free spare parts. This means you can predict your running costs precisely and budget for them. We are here for you: 13
14 The moment technology provides you with a result the first time. This is the moment we work for. // CONFIDENCE made by CARL ZEIss 14
15 facebook.com/zeissmicroscopy twitter.com/zeiss_micro youtube.com/zeissmicroscopy e CZ-IX/2011 Design, scope of delivery and technical progress subject to change without notice. Carl Zeiss Microscopy GmbH Carl Zeiss Microscopy GmbH Jena, Germany BioSciences
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