Microscope Review. 1. A compound light microscope is represented in the diagram below.

Transcription

1 Name Microscope Review Date 1. A compound light microscope is represented in the diagram below. 5. The diagram below represents a hydra as viewed with a compound light microscope. If the hydra moves toward the right of the slide preparation, which diagram best represents what will be observed through the microscope? A) B) Which microscope part is correctly paired with its function? A) A magnifies the image of the specimen B) B used for focusing only when the high-power objective is used C) C provides the field of view with the largest diameter D) D holds the specimen on the stage 2. A microscope with 10 and 40 objectives is used to scan a slide to locate a specimen. The largest field of vision would be provided by using the C) D) 6. A student studied the upper layer of cells of a tissue sample under the high-power objective of the compound light microscope shown below. A) coarse adjustment B) widest diaphragm opening C) 10 objective D) 40 objective 3. The structure of a cell nucleus would be seen in the greatest detail by use of A) a compound light microscope B) an ultracentrifuge C) a dissecting microscope D) an electron microscope 4. A student using a compound microscope measured the diameter of several red blood cells and found that the average cell length was millimeter. What is the average length of a single red blood cell in micrometers? A) 0.8 B) 8 C) 80 D) 800 Which part of the microscope should the student adjust to observe the cells in the lower layer of the sample? A) A B) B C) C D) D

2 7. The diagram at the right represents a strand of algae viewed under the low-power objective of this microscope. Under the high-power objective, how would this same slide appear? A) B) C) D) 11. To locate a specimen on a prepared slide with a compound microscope, a student should begin with the low-power objective rather than the high-power objective because the A) field of vision is smaller under high power than under low power B) field of vision is smaller under low power than under high power C) specimen does not need to be stained for observation under low power but must be stained for observation under high power D) portion of the specimen that can be observed under low power is less than the portion that can be observed under high power Base your answers to questions 8 and 9 on the diagram below and on your knowledge of biology. 12. A student sees the image above when observing the letter "f" with the low-power objective lens of a microscope. Which diagram below most closely resembles the image the student will see after switching to high power? A) B) C) D) 13. The field of view becomes darker when a compound microscope is switched from low to high power. The field of view can then be made brighter by 8. Which part must be adjusted to enable the viewer to continually observe a paramecium swimming at changing levels in a wet mount under high power? A) decreasing the size of the diaphragm opening B) increasing the size of the diaphragm opening C) refocusing with the fine adjustment D) refocusing with the coarse adjustment 14. What is the approximate diameter of the cell shown in the low-power field of a compound light microscope represented below? A) 7 B) 2 C) 3 D) 8 9. After part 3 is turned, which part must often be adjusted? A) 1 B) 5 C) 6 D) A student views some cheek cells under low power. Before switching to high power, the student should A) adjust the eyepiece B) center the image being viewed C) remove the slide from the stage D) remove the coverslip A) 100 µm B) 500 µm C) 800 µm D) 1,000 µm 15. Which microscope magnification should be used to observe the largest field of view of an insect wing? A) 20 B) l00 C) 400 D) 900

3 16. The diagram below shows three cells in the field of view of a microscope. The diameter of the field of view is 1.5 millimeters. 21. The white blood cell represented below measures 0.02 millimeter from point A to point B. What is the approximate diameter of each cell? A) 50 µm B) 250 µm C) 500 µm D) 4500 µm 17. A student calculated the diameter of the high power field of a compound light microscope to be 0.5 millimeter. If 10 plant cells fit end to end across the diameter of the high-power field, the average length of each plant cell would be The length of the bacterium is approximately A) 2000 µm B) 500 µm C) 20 µm D) 5 µm 22. The diagram below represents a portion of a metric ruler as seen with a compound microscope. A) 50 mm B) 5 mm C) 200 mm D) 20 mm 18. The diagram below represents cells seen in the low-power field of view of a compound light microscope. The diameter of the field of view illustrated is approximately The length of one of these cells is approximately A) 200 µm B) 400 µm C) 500 µm D) 2,000 µm 19. A student determined that exactly 10 pollen grains could fit along the diameter of the field of view of a microscope. If each pollen grain has a diameter of 200 micrometers, what is the diameter in millimeters of this microscope's field of view? A) 2,000 mm B) 2 mm C) 5 mm D) 500 mm 20. The 10 objective of a compound microscope is being used to observe a specimen. If a total magnification of 150 is achieved, then the magnification of the ocular of this microscope must be A) 10 B) 15 C) 150 D) 1500 A) 1,600 mm B) mm C) 1,600 µm D) 2,000 µm 23. Each division of the metric ruler shown in the diagram below equals 1 millimeter. The diameter of the field of vision is approximately A) 2,800 µm B) 3,700 µm C) 4,400 µm D) 4,700 µm 24. What is the highest magnification that can be obtained with a microscope that has a 10 eyepiece with 10 and 43 objectives? A) 43 B) 100 C) 430 D) 4300

4 25. The diagram below represents a specimen in the low-power field of view of a compound light microscope. If the slide is not moved, which view best represents the way the specimen will look when the high-power objective lens is switched into place? A) B) C) D) 26. Base your answer to the following question on the information and diagrams below and on your knowledge of biology. The drawings below were made during a laboratory exercise in which a microscope was used to view slides of preserved protozoa. The microscope had a 10x eyepiece and two different objectives. Which statement about the size of the organisms is correct? A) Organism A is larger than organism B. B) Organism B is larger than organism A. C) Organisms A and B are both the same size. D) The relative size of the organisms cannot be determined from the information given.

5 Base your answers to questions 27 and 28 on the diagram of a microscope below and on your knowledge of biology. 30. Figure A represents a cell as viewed by a student using the 10 ocular and the 10 objective of a compound light microscope. Figure B represents the same cell as seen with a different objective. The magnification of the objective used to observe the cell shown in figure B is most likely 27. Which lettered part should be used to focus the image while using high power? A) 4 B) 40 C) 60 D) What is the lowest possible magnification that can be obtained using the microscope shown below? 28. Information about which two lettered parts is needed in order to determine the total magnification of an object viewed with the microscope in the position shown? 29. Base your answer to the following question on the diagram below of a microscope and on your knowledge of biology. A) 20 B) 200 C) 40 D) A compound microscope has four objectives labeled 4, 10, 43, and 97. Which objective, when used in combination with a 10 ocular lens, provides the largest field of view? The highest possible magnification that can be obtained when using this microscope is A) 40 B) 100 C) 400 D) 4,000 A) 97 B) 43 C) 10 D) Which part of a compound light microscope should a student adjust to allow more light to pass through a specimen? A) fine adjustment B) ocular C) diaphragm D) stage

6 34. The diagrams below show four different one-celled organisms (shaded) in the field of view of the same microscope using different magnifications. Which illustration shows the largest one-celled organism? A) B) C) D) 35. What information from the list below must be used to estimate the length of a cell under the low-power objective of a compound light microscope? Information A Type of cell in the field B Magnification of the low-power objective C Magnification of the high-power objective D Diameter of the low-power field of view E Number of cells that fit end-to-end across the diameter of the low-power field of view A) A and C, only B) A, B, C, and E, only C) D and E, only D) A, B, C, D, and E 36. A student used a light microscope to observe a cell under low power. After the student switched to high power and attempted to focus, the cell was no longer visible. What was most likely the cause of the disappearance of the cell? A) The diaphragm was open while the student observed the cell under low power. B) The distance between the specimen and the objective lens decreased after the student switched to high power. C) The student focused the eyepiece before observing the cell under high power. D) The cell was not in the center of the field of view when the student observed it under low power. 37. Which activity might lead to damage of a microscope and specimen? A) cleaning the ocular and objectives with lens paper B) focusing with low power first before moving the high power into position C) using the coarse adjustment to focus the specimen under high power D) adjusting the diaphragm to obtain more light under high powe 38. A student prepared a slide of pollen grains from a flower. First the pollen was viewed through the low-power objective lens and then, without moving the slide, viewed through the high- power objective lens of a compound light microscope. Which statement best describes the relative number and appearance of the pollen grains observed using these two objectives? A) low power: 25 small pollen grains; high power: 100 large pollen grains B) low power: 100 small pollen grains; high power: 25 large pollen grains C) low power: 25 large pollen grains; high power: 100 small pollen grains D) low power: 100 large pollen grains; high power: 25 small pollen grains 39. After switching from the high-power to the low-power objective lens of a compound light microscope, the area of the low-power field will appear A) larger and brighter B) smaller and brighter C) larger and darker D) smaller and darker 40. A slide of human blood cells was observed in focus under the low-power objective of a compound light microscope that had clean lenses. When the microscope was switched to high power, the image was dark and fuzzy. Which parts of the microscope should be used to correct this situation? A) nosepiece and coarse adjustment B) diaphragm and ocular C) objective and fine adjustment D) diaphragm and fine adjustment 41. Which sequence contains the correct order of steps for a student to follow to observe the nucleus of protozoa in a stained wet mount, using a compound light microscope? A) 1 B) 2 C) 3 D) Which part of a light microscope would most likely be damaged if the coarse adjustment is improperly used while a specimen is being observed under high power? A) objective lens B) light source C) iris diaphragm D) eyepiece lens

7 43. A student observed several cell layers positioned on top of one another in the high-power field of view of a compound light microscope. To observe the details of only one of these cell layers, the student should 47. Base your answer on the diagram below of a compound light microscope on your knowledge of biology. A) move the slide from left to right B) adjust the diaphragm to a smaller opening C) rotate the fine adjustment D) turn the ocular Base your answers to questions 44 and 45 on the diagrams below and on your knowledge of biology. 44. One similarity between all the microscopes shown is that they A) lack a diaphragm and coarse adjustment B) have three oculars C) depend on electrons passing through the specimen D) have two objective lenses 45. Which microscope setup would allow a student to see the largest field? A) A B) B C) C D) D To locate and observe a specimen on a slide with this microscope, a student should begin by using A) the coarse adjustment and the low-power objective B) the fine adjustment and the high-power objective C) a closed diaphragm and the low-power objective D) high light intensity and the high-power objective 48. The diagram below illustrates a part of a hydra as seen with the low-power objective of a compound microscope. The diameter of the low-power field of view is 1.4 millimeters. 46. The diagram below shows a cell as seen in the low-power field of a compound microscope. The length of one of the tentacles is closest to In which direction should the slide be moved to center the cell in the field of view? A) 300 µm B) 700 µm C) 1,100 µm D) 1,400 µm A) toward 1 B) toward 2 C) toward 3 D) toward 4

8 49. According to the diagram below, the diameter of the black circle shown in the field of view of the microscope is closest to 50. Base your answer to the following question on the diagram of a compound light microscope below and on your knowledge of biology. A) 200 µm B) 600 µm C) 800 µm D) 1,400 µm Which parts of this compound light microscope with clean lenses should be used to improve the quality of the image being observed in the high-power field of view? A) ocular and high-power objective B) diaphragm and fine-adjustment knob C) coarse-adjustment knob and low-power objective D) ocular and diaphragm

9 Answer Key Microscope Quiz* 1. A 2. C 3. D 4. B 5. B 6. B 7. D 8. B 9. D 10. B 11. A 12. A 13. B 14. B 15. A 16. C 17. A 18. B 19. B 20. B 21. D 22. C 23. B 24. C 25. A 26. A 27. E 28. A and C 29. C 30. A 31. B 32. D 33. C 34. C 35. C 36. D 37. C 38. B 39. A 40. D 41. A 42. A 43. C 44. D 45. A 46. D 47. A 48. B 49. C 50. A