Specifications of the Instruments:

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Wendy Dorsey
6 years ago
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1 SHORT QUOTATION NOTICE TU/11-24/Pur/Qtn/2010/ 2148-A, Dt:22/8/11 Sealed Quotations are invited from manufacturer, dealers, etc. for supply of Laboratory equipments for DBT Twinning Project of Professor A. K. Buragohain. Last date of submission of quotations is 19/9/11. Registrar Memo No. TU/11-24/Pur/Qtn/2010/ 2148-A Dt:22/8/11 Specifications of the Instruments: 1)Specification of Real Time PCR Real time PCR with block of 96 x 0.2 ml/48x0.1ml tubes or plate to Run typical 0.2ml/0.1ml tubes, strips, and plates. The base thermal cycler should be able to be used for standard PCR Detection of two/three different fluorescent reporters in the same tube. Two Excitation and Emission Filters should be included with fluorescence excitation range of nm and detection range: nm in one channel and nm in the other channel. Should be capable of detecting 1 copy number of genomic DNA under standard conditions Maximum Ramping speed : >2.5-3 C per sec Peltier / Joule heating and cooling for uniform temperature control

2 Internal reference dye should be required. True 2/3 Color Multiplexing with use of 2/3 different flourophores with the need of addition of any internal reference dye. Tungsten-halogen/LED as excitation source and CCD/photodiode as detector Open system capable of running various chemistries Temperature range OC with accuracy of ±0.3 OC and uniformity of ±0.4 C within 10 sec of arrival at 90 C Sample Size : μl Automatic allelic discrimination by end point fluorescence or threshold cycle Absolute quantitation with standard curve Gene expression analysis by relative quantity (ΔCt ) or normalized expression(δδct) with multiple reference genes End point analysis for upto 2 fluorophores Should have mode for Melt curve analysis Multiple gene expression analysis for comparison of over 5,000 Ct values from different data files should be possible System should be capable for detection of dyes- SYBR GREEN I, ROX, FAM,VIC,JOE, on a single plate. The data collection and instrument control software should provide multicomponenting algorithm for deconvoltion of multiple dye signal with minimum cross talk 2) Trinocular Inverted Phase Contrast & Fluorescence Research Microscope with Built in Camera Port and Digital Photography System Sturdy stand with built-in 12V 35W halogen light illumination. 5x nosepiece to accommodate five different objectives. Large Specimen stage of minimum 210 x 290 mm to accommodate different samples Viz., large tissue culture bottles, petri dishes and culture flasks. Built-in documentation port at the front for attaching SLR camera or Digital / CCD Camera systems to ensures space saving design and easy access to the work area of the stage.

3 Universal mounting frame suitable for holding specimen slide and petri dishes of minimum dia mm shall be available as an option. Conversion filter K for photomicrography and green filter for phase contrast. Objectives suitable for phase contrast and bright field. Plan Achromat Objectives A-Plan 10x/0.25 Ph 1, LD A-Plan 20x/0.30 Ph 1 and LD A-Plan 40x/0.50 Ph 2 with XY movement of Specimen Stage. Universal Condenser for bright field and phase contrast. This Condenser shall have a comfortable working distance of minimum 53mm to make the Microscope upgradable for IVF and Micromanipulation work. The Microscope shall have fluorescence attachment. The fluorescence illumination light path shall be of Light Trap technology to get a good contrast in the final image. The filter sets shall be easily insertable and removable by Push & Click method The Microscope shall have provision to up-grade to DIC, preferably Plas DIC for getting relief contrast of cells in plastic container. Scientific Digital Monochrome 1388 (H) x 1038 (V) Camera = 1.4 Mega Pixel, Pixel Size: 4.65 µm x 4.65 µm, Chip size:, ½, Spectral range: 400 to 1000 nm with protection glass, H x V Frame 1388 x fps or equivalent, Digitization: 12 bit pixel, Registration: CE or equivalent, Power Supply: via FireWire interface, Dual Chip Firewire card Environmental conditions: Celsius, Navigator windows, Interactive measurement: length, contours, angles, Management, visualization and printing of metadata and images, µ bar scales, Text annotation, Image printout, Image properties with display functions, automatic saving, Image File Browser, Postprocessing of images; standard operations for image optimization contrast, brightness, gamma, colors etc. Branded Desk top Computer Dell, IBM, HP, ACER, WIPRO, HCL or equivalent. Core 2 Duo, C2D 2.66 GHz, 400 GB SATA HDD, 4 GB RAM DDR2, 18.5 / 19 TFT Monitor, DVD Writer, Optical Mouse, Key Board, or equivalent latest version, HP / Canon / Epson Desk Jet Printer. Microscope, Camera, Software should preferably be from same manufacturer for better integration and after sales support. 3) Sonicator Name of the Item Specifications(essential) Desirable

4 SONICATOR 1. The Sonicator must have shorter processing times and greater sample consistency 2. Should be simple and effective in operation 3. Should possess thumb switch to hold probe and deliver precise energy. 4. Should allow close monitoring of sample. 5. Should have auto tuning facility 6. Should have programmable timer and adjustable pulse. 1. Should include probe for processing samples of different volumes (0.5-15ml and 10-50ml) 2. Also should include Sound proof box. 4) Laminar Flow Name of the Item LAMINAR FLOW Specifications(essential) 1. The laminar flow should be of a work area of 4 x2 x2. 2. It should be acid, pest and weather resistant. 3. Should be RUST FREE. 4. Should have efficient HEPA filter to retain particulates and biological contaminants larger than 0.3 microns. 5. The glass sheet should be clear and UV protective. 6. Should include UV tube, Fluorescent tube light, Gas cock, manometer and complete CP fitting. 7. Should work on 220/230 V AC 50HZ. GENERAL TERMS & CONDITIONS: 1. No separate tender paper will be issued from the office: one should only download the List(s) from the website.

5 2. The rates should be quoted on FOR Tezpur University, Napaam Basis. 3. Quotations should be accompanied byi) A call deposit (earnest money) amounting 5,000/- pledge in favor of Registrar, Tezpur University, payable at Tezpur. 4. The rates should be exclusive of taxes and applicable tax% should be clearly indicated. 5. Quoted rates should be valid at least for a period of 6 months. 6. The rates should quoted along with supporting documents of specifications and technical features and list of users. 7. Vendor to provide point to point technical compliance statement. Details of availability of after sales support will have to be furnished. After sales support directly from manufacturer and from Assam ( Guwahati / Tezpur) will be preferred. 8. The University is exempted from paying Custom and Excise duty. 9. Proprietary items should be quoted with sole Manufacturer/Distributorship certificate. 10. No Advance payment will be made. However if the item is of foreign origin, advance payment will be made either by FDD or vide LOC. In such cases 10% Performance Bank Guarantee should be submitted before issuing FDD/LOC, covering the warranty period. 11. The University reserves the right to accept or reject any or all the quotations without assigning any reason. 12. Applications should be forwarded to the Registrar for release of call deposit against unaccepted quotations. 13. Without application EMD will not be released. Registrar.

SPECIFICATION OF SOLAR CELL TESTER

SPECIFICATION OF SOLAR CELL TESTER A. Purpose: This equipment is to be used for testing of mono and multi crystalline silicon solar cells under standard testing conditions. The equipment shall be interfaced