Lab 1, 2 and 3: Stain, Observe and Identify the Microbes. BIOHAZARD Rules. VIOLATORS will lose points. A) Lab Safety Rules Lab Safety Form Signup
|
|
|
- Marianna Blair
- 5 years ago
- Views:
Transcription
1 MICROLAB PREPARATIONS A) Lab Safety Rules Lab Safety Form Signup B) Lab Participation Instructor Review Peer Review Form C) Biohazard Rules How to dispose Trash REQUIRED Items: 1) LAB Manual/Journal 2) Scantrons 882-E, No.2 pencil USEFUL Items 1) Colored Pencils Purple/Pink/Red/Blue/Green BIOHAZARD Rules VIOLATORS will lose points C1) NO FOOD/DRINK ALLOWED in LAB C2) CORRECT TRASH in CORRECT CONTAINER Do not put regular trash in any of the biohazard bins All laboratory supplies (which touches live biospecimen) and biospecimen must be placed in the BIO HAZARD bin. C2A) BIOHAZARD Cardboard Container (plastic/paper) C2B) BIOHAZARD Plastic Container (glass culture vials) you will learn the basic skill sets necessary to Lab 1, 2 and 3: Stain, Observe and Identify the Microbes ACGM/CSLO Goals: 1. Use and comply with laboratory safety rules, procedures, and universal precautions. 2. Demonstrate proficient use of a compound light microscope. 3. Describe and prepare widely used stains and wet mounts, and discuss their significance in identification of microorganisms. 4. Perform basic microbiology procedures using aseptic techniques for transfer, isolation and observation of commonly encountered, clinically significant bacteria. 1
2 Figure 3.4 Approximate size of various types of cells. ~25 um Red Blood Cells 1.5mm 1500 um = 1500 Width of penny Figure 4.3 The limits of resolution (and some representative objects within those ranges) of the human eye and of various types of microscopes. 3 General Principles of Microscopy Magnification Resolution Contrast Official definitions Magnification, the ratio of an object s image size to its real size Resolution, the measure of the clarity of the image, or the minimum distance of two distinguishable points Contrast, visible differences in brightness between parts of the sample 2
3 3 General Principles of Microscopy A) Magnification (enlarge) B) Resolution (tell apart 2 objects close together) C) Contrast (Differences in intensity between two objects) How to increase all 3 of these? A) Magnification vs B) Resolution High Magnification but Low Resolution How to increase A) Magnification and B) Resolution VIBGYOR Smaller the object = Use smaller wavelength 3
4 50 µm General rule for any microscopy/detector Smaller the wavelength smaller the object you can see Light vs Electron microscope Visible Light Electron has very small wavelength compared to visible light Light vs Electron microscope Visible Light Electron Magnifies objects 1000X Magnifies objects 100,000X C) Contrast (Differences in intensity between two objects) Staining increases contrast Brightfield (unstained specimen) Brightfield (stained specimen) 4
5 Figure 4.16 Simple stains. Light Microscopy In a light microscope (LM), visible light is passed through a specimen and then through glass lenses Glass lenses focuses light and enlarges and resolves objects Light changes direction (refracts) when it moves from one medium to another Light Air Air Glass 5
6 polish glass Magnification = 50/5 = 10X Focal point 5 50 Specimen Convex lens Magnify using lenses Inverted, reversed, and Enlarged image Light Microscopy Bright-field microscopes Simple Microscopes Contain a single magnifying lens Similar to magnifying glass Leeuwenhoek used simple microscope to observe microorganisms for the first time in history Fresh pond water Light Microscopy (Bright-field microscopes) Compound multiple lenses Series of lenses for magnification Light passes through specimen into objective lens Have one or two ocular lenses Total magnification = magnification of objective lens X magnification of ocular lens e.g. 10 times X 10 times = 100 times 6
7 Figure 4.4 A bright-field, compound light microscope. occular lens Line of vision Ocular lens Remagnifies the image formed by the objective lens Body Transmits the image from the objective lens to the ocular lens using prisms Arm Objective lenses Primary lenses that magnify the specimen Stage Holds the microscope slide in position Condenser Focuses light through specimen Diaphragm Controls the amount of light entering the condenser Illuminator Light source Ocular lens Path of light Prism Body Objective lenses Specimen Condenser lenses Illuminator Coarse focusing knob Moves the stage up and down to focus the image Fine focusing knob Base objective lens Figure 4.5 The effect of immersion oil on resolution. Oil immersion lens (100X) is a special type of objective lens Microscope objective Lenses Microscope objective Refracted light rays lost to lens Glass cover slip More light enters lens Glass cover slip Immersion oil Slide Slide Specimen Light source Without immersion oil Light source With immersion oil Oil immersion lens Increases magnification and resolution Light changes direction (refracts) when it moves from one medium to another Light Air Air Glass 7
8 50 µm Figure 4.5 The effect of immersion oil on resolution. Oil immersion lens (100X) is a special type of objective lens Microscope objective Lenses Microscope objective Refracted light rays lost to lens Glass cover slip More light enters lens Glass cover slip Immersion oil Slide Slide Specimen Light source Without immersion oil Light source With immersion oil Oil immersion lens Increases magnification and resolution Staining increases contrast Brightfield (unstained specimen) Brightfield (stained specimen) Light Microscopy Staining Principles of Staining Dyes used as stains are usually salts Chromophore is the colored portion of the dye Basic dyes Basic dyes (positively charged) stain acidic (negatively charged) structures Acidic dyes (negatively charged) stain alkaline structures 8
9 Inside of most bacterial cells is negatively charged + Basic dyes Therefore Basic dyes are more commonly used to stain bacterial cells Positive Staining Inside of most bacterial cells is negatively charged Acidic dyes Therefore Acidic dyes are more commonly used to stain background Negative Staining Simple/Differential Staining? Positive/Negative Staining? 9
10 Simple/Differential Staining? Positive/Negative Staining? Negative staining Rhodospirillum rubrum R. rubrum Rhodospirillum rubrum R. rubrum This large ( mm), nonpathogenic gram negative spirillum exhibits polar amphitrichous flagellation and a characteristic red pigment. 10
11 Binomial nomenclature naming species of living things by giving each species a name composed of two parts humans belong to the genus Homo species Homo sapiens. Homo sapiens noun-genus Capitalized adjective-specific epithet Homo sapiens Homo naledi (2015) Microbe Naming Rules Each species has a unique name Genus specific epithet G. specific epithet Genus specific epithet G. specific epithet Escherichia coli Escherichia coli E. coli E. coli 11
12 X Microbe Naming Rules Genus specific epithet X Genus specific epithet Y Genus specific epithet Y Genus specific epithet Bacteria have shapes/morphology Figure 3.12 Bacterial shapes and arrangements. 3 major shapes 1) coccus 2) bacillus 3) spirillum 1) staphylo 2) strepto 2 major arrangements 12
13 cocci bacilli shape arrangement Streptococci Streptobacilli Staphylococci Microbe Naming Rules based on shape and arrangement (Arrangement+shape) (specific epithet) Streptobacillus (specific epithet) Staphylococcus (specific epithet) Light Microscopy Staining Simple stains = single stains Can be used for Positive or Negative Stains Positive Stain = any bacteria Negative Stain = any bacteria/capsule/flagella 13
14 Light Microscopy Staining Differential stains = multiple stains Gram stain/acid-fast stain/endospore stain Gram stain Microscopy Light Microscopy 1) Simple Staining Preparing a specimen for staining Aseptic technique Innoculating loop Inoculum (small amount of culture) 14
15 Prokaryotic Microbes Bacteria with 3 shapes/morphology Prokaryotic Microbes bacteria Bacteria cell model 15
16 Bacteria Prokaryotic Microbes vs Eukaryotic Microbes Protozoa - Paramecium Algae - Spirogyra Fungus - Yeast Eukaryotic Microbes paramecium 16
17 Eukaryotic Microbes spirogyra Eukaryotic Microbes yeast Culturing Microorganisms Culture Act of cultivating microorganisms or the microorganisms that are cultivated Mixed Culture vs Pure Culture Inoculum (small amount of culture) introduced into medium 17
18 Only live bacteria will grow and form colony Inoculum (small amount of culture) 1 bacteria = 1 COLONY Therefore bacteria also called CFU = COLONY forming unit colony composed of cells arising from a single progenitor therefore progenitor bacteria is termed a colony-forming unit (CFU) 10 COLONIES Therefore original culture had how many COLONY forming units? How many live bacteria? Mixed Culture 1 bacteria = 1 COLONY Separate bacteria from each other Mixed Culture? Pure Culture 18
19 2 methods to acquire pure cultures from mixed cultures Streak plates Pour plates The streak-plate method of isolation Can individually pick single cell of some large microorganisms and use to establish a culture The pour-plate method of isolation Sequential inoculations 1.0 ml 1.0 ml 1.0 ml Initial sample 9 ml broth 9 ml broth 9 ml broth 1.0 ml to each Petri dish, add 9 ml warm agar, swirl gently to mix Colonies Fewer colonies 19
Microscopy, Staining, and Classification
PowerPoint Lecture Presentations prepared by Mindy Miller-Kittrell, North Carolina State University C H A P T E R 4 Microscopy, Staining, and Classification Figure 3.4 Approximate size of various types
Figure 3.4 Approximate size of various types of cells. ~10 um. Red Blood Cells = mm 1500 um. Width of penny Pearson Education, Inc.
Figure 3.4 Approximate size of various types of cells. ~10 um Red Blood Cells 1.5mm 1500 um Width of penny = 1500 Figure 4.3 The limits of resolution (and some representative objects within those ranges)
Microbiology: Observing Bacteria Laboratory -1. Name Date
Microbiology: Observing Bacteria Laboratory -1 Name Date Prelab: Part 1 Introduction to the microscope- please read through this handout and label the picture on the next page before starting the lab Care
Scale. A Microscope s job in life. The Light Microscope. The Compound Microscope 9/24/12. Compound Microscope Anatomy
The Study of Microbial Structure: Microscopy and Specimen Preparation Scale A Microscope s job in life 1.Magnify 2. Resolve ability to separate or distinguish between two points 3. Contrast How much or
Microbiology Laboratory 2
Microbiology Laboratory 2 Microscopy Background Microorganisms are too small to be seen with the naked eye. Thus a microscope is used to magnify objects so they can be observed. A lens consists of one
Ex 1: Introduction to the microscope
Ex 1: Introduction to the microscope So what exactly is a microorganism? Microorganisms = any living thing that is too small to be seen with the unaided eye fungus protist bacteria virus Parasitic worm
Observing Microorganisms through a Microscope
2016/2/19 PowerPoint Lecture Presentations prepared by Bradley W. Christian, McLennan Community College CHAPTER 3 Observing Microorganisms through a Microscope 1 Figure 3.2 Microscopes and Magnification.
THE COMPOUND BRIGHTFIELD MICROSCOPE
THE COMPOUND BRIGHTFIELD MICROSCOPE Microbiology is the study of microscopic organisms that are so small that they are below the limit of vision of the human eye. Bacteria are the smallest of microorganisms
Chapter 3. Observing Microorganisms Through a Microscope
Chapter 3 Observing Microorganisms Through a Microscope Microbial Size Macroscopic organisms can be measured in the range from meters (m) to centimeters (cm) Microscopic organisms fall into the range
MICROSCOPY MICROSCOPE TERMINOLOGY
1 MICROSCOPY Most of the microorganisms that we talk about in this class are too small to be seen with the naked eye. The instruments we will use to visualize these microbes are microscopes. The laboratory
Lab 2 T. Microbes in Everyday Life; Pure Culture Project; Hand Washing; Light Microscopy
Microbes in Everyday Life; Pure Culture Project; Hand Washing; Light Microscopy Lab 2 T oday s lab looks at the results of microbial diversity and continues the project of working toward producing a pure
LAB 1 Introduction to Microscopy
I. Ubiquity of Microorganisms II. Microscopy LAB 1 Introduction to Microscopy I. UBIQUITY OF MICROORGANISMS Microorganisms are ubiquitous; that is, they are present nearly everywhere. In this lab you will
A BRIEF INTRODUCTION TO MICROSCOPY The two key properties of a microscope that allow you to see microbes are resolution and magnification.
A BRIEF INTRODUCTION TO MICROSCOPY The two key properties of a microscope that allow you to see microbes are resolution and magnification. Magnification refers to the enlargement of the specimen when seen
Exercise 2-A MICROSCOPIC TECHNIQUE & EXAMINATION OF MICROORGANISMS
Exercise 2-A MICROSCOPIC TECHNIQUE & EXAMINATION OF MICROORGANISMS Introduction to Microscopic Technique Microbiology is the science or study of living organisms too small to be seen with the naked eye.
Burton's Microbiology for the Health Sciences
Burton's Microbiology for the Health Sciences Chapter 2. Viewing the Microbial World Chapter 2 Outline Introduction Using the metric system to express the sizes of microbes Microscopes Simple microscopes
Exercise 2-A MICROSCOPIC TECHNIQUE & EXAMINATION OF MICROORGANISMS
Exercise 2-A MICROSCOPIC TECHNIQUE & EXAMINATION OF MICROORGANISMS Introduction to Microscopic Technique Microbiology is the science or study of living organisms too small to be seen with the naked eye.
Biology 29 Cell Structure and Function Spring, 2009 Springer LABORATORY 1: THE LIGHT MICROSCOPE
Biology 29 Cell Structure and Function Spring, 2009 Springer LABORATORY 1: THE LIGHT MICROSCOPE Prior to lab: 1) Read these instructions (p 1-6) 2) Go through the online tutorial, the microscopy pre-lab
2018 MICROSCOPE REVIEW by Karen L. Lancour RELATIVE SIZE OF MICROBES
2018 MICROSCOPE REVIEW by Karen L. Lancour RELATIVE SIZE OF MICROBES 1000 millimeters (mm) = 1 meter (m) 1000 micrometers (µm or mcm) = 1 millimeter (mm) 1000 nanometers (nm) = 1 micrometer (mcm) Size
Laboratory Introduction
Laboratory Introduction There are two basic categories of microscopes: light microscopes and electron microscopes. Light, or optical, microscopes require light waves to provide the illumination while electron
The Care and Use of the Microscope. Lab Exercise #4
Lab Safety No eating or drinking!!! Long hair must be tied back Clean up your workstation before you leave! Return all materials to the storage sites Clean glassware and wipe down countertops Follow directions
2017 MICROSCOPE REVIEW by Karen L. Lancour RELATIVE SIZE OF MICROBES
2017 MICROSCOPE REVIEW by Karen L. Lancour RELATIVE SIZE OF MICROBES 1000 millimeters (mm) = 1 meter (m) 1000 micrometers (µm or mcm) = 1 millimeter (mm) 1000 nanometers (nm) = 1 micrometer (mcm) Size
Compound Light Microscopy. Microscopy. Things to remember... 1/22/2017. This is what we use in the laboratory
Compound Light Microscopy This is what we use in the laboratory Microscopy Chapter 3 BIO 440 A series of finely ground lenses is used to form a magnified image Specimen is illuminated with visible light
The microscope is useful in making observations and collecting data in scientific experiments. Microscopy involves three basic concepts:
AP BIOLOGY Chapter 6 NAME DATE Block MICROSCOPE LAB PART I: COMPOUND MICROSCOPE OBJECTIVES: After completing this exercise you should be able to: Demonstrate proper care and use of a compound microscope.
Observing Microorganisms through a Microscope LIGHT MICROSCOPY: This type of microscope uses visible light to observe specimens. Compound Light Micros
PHARMACEUTICAL MICROBIOLOGY JIGAR SHAH INSTITUTE OF PHARMACY NIRMA UNIVERSITY Observing Microorganisms through a Microscope LIGHT MICROSCOPY: This type of microscope uses visible light to observe specimens.
King Saud University Dept. of Bot. & Microbiology. General Microbiology 140 MIC
King Saud University Dept. of Bot. & Microbiology General Microbiology 140 MIC Lab coat. Do not wearing the lab coat outside the lab. Gloves. Proper Clothing and closed shoes. Hair should be tied back.
Match the microscope structures given in the left column with the statements in the right column that identify or describe them.
49 Prelab for Name Match the microscope structures given in the left column with the statements in the right column that identify or describe them. Key: a. coarse adjustment knob f. turret or nosepiece
MICROSCOPE LAB. Resolving Power How well specimen detail is preserved during the magnifying process.
AP BIOLOGY Cells ACTIVITY #2 MICROSCOPE LAB OBJECTIVES 1. Demonstrate proper care and use of a compound microscope. 2. Identify the parts of the microscope and describe the function of each part. 3. Compare
THE BRIGHT-FIELD MICROSCOPE: ITS PROPER USE AND CARE
Microbiology Laboratory (BIOL 3702L) Page 1 of 17 THE BRIGHT-FIELD MICROSCOPE: ITS PROPER USE AND CARE The microscope is the basic instrument used to visualize cells and their structures. In the field
Microscopy Techniques that make it easy to see things this small.
Microscopy Techniques that make it easy to see things this small. What is a Microscope? An instrument for viewing objects that are too small to be seen easily by the naked eye. Dutch spectacle-makers Hans
MICROSCOPY and CELL STRUCTURE
MICROSCOPY and CELL STRUCTURE Readings: Review pp. 69-71, and Fig. 4.1 on p. 65 in your text (POHS, 5 th ed.). Introduction: Biologists rely on many different types of microscopic techniques to find out
Microscopy. Krishna Priya.K Lecturer Dept. of Microbiology
Microscopy Krishna Priya.K Lecturer Dept. of Microbiology TERMS AND DEFINITIONS Principle Microscopy is to get a magnified image, in which structures may be resolved which could not be resolved with the
2/4/15. Brightfield Microscopy! It s all about Magnification..! or is it?!
Brightfield Microscopy It s all about Magnification.. or is it? 1 What actually does go into chosing a microscope Choice depends on what you need the microscope to do. Do you want to magnify stained specimens?
Name: Date Completed: Class: Lab Minutes: Teacher:
Name: Date Completed: _ Class: Lab Minutes: _ Teacher: Introduction to the Microscope Lab Activity This lab was created by Mr. Buckley from Edward Knox High School. Credit is given for this original activity
Protist Microscope Lab
Name: Block: Due Date: Protist Microscope Lab Pre-Lab Assignment 1. Fill out the table for question #4 on the second page of your lab packet. (You may use the Biology textbook pages R8 and R9 in the back
The microscope is useful in making observations and collecting data in scientific experiments. Microscopy involves three basic concepts:
Lab #4 Biology 10 BCC Topic: MICROSCOPE LAB PART I: COMPOUND LIGHT MICROSCOPE OBJECTIVES: After completing this exercise you should be able to: Demonstrate proper care and use of a compound microscope.
LAB 3 Use of the Microscope
LAB 3 Use of the Microscope Introduction In this laboratory you will be learning how to use one of the most important tools in biology the compound light microscope to view a variety of specimens. You
Chapter 2 The Study of Microbial Structure: Microscopy and Specimen Preparation
Chapter 2 The Study of Microbial Structure: Microscopy and Specimen Preparation 1 Lenses and the Bending of Light light is refracted (bent) when passing from one medium to another refractive index a measure
Visual Anatomy ansd Physiology Lab Manual Pig Version 2nd Edition Sarikas TEST BANK
Visual Anatomy ansd Physiology Lab Manual Pig Version 2nd Edition Sarikas TEST BANK https://testbankreal.com/download/visual-anatomy-ansd-physiology-labmanual-pig-version-2nd-edition-sarikas-test-bank/
Introduction to the Compound Microscope Cell Structure & Function
Introduction to the Compound Microscope Cell Structure & Function Revised Fall 2018 Laboratory Safety Lab coat, long pants, closed-toe shoes, safety goggles, and nitrile or latex gloves are required. **You
Microscopy http://www.microscopyu.com/articles/phasecontrast/phasemicroscopy.html http://micro.magnet.fsu.edu/primer/anatomy/anatomy.html 2005, Dr. Jack Ikeda & Dr. Gail Grabner 9 Nikon Labophot (Question
What you should have learned from the microscope labs.
What you should have learned from the microscope labs. Microscope Lab 1 Directionality Items appear backwards and inverted On Stage In Microscope NOT!!!! Microscope Lab 1 More Directionality Items move
Unit Two Part II MICROSCOPY
Unit Two Part II MICROSCOPY AVERETT 1 0 /9/2013 1 MICROSCOPES Microscopes are devices that produce magnified images of structures that are too small to see with the unaided eye Humans cannot see objects
Microscope Notes. units of life.
Microscope Notes Microscope an instrument that produces an enlarged image of an object. Biologists use microscopes to study cells, cell parts, and organisms that are too small to be seen with the naked
Introduction. Instructional Objectives. Materials. Procedure. I. Microscope Parts and Function. Honors Biology
Honors Biology Introduction to the Microscope Lab Activity This lab was created by Mr. Buckley from Edward Knox High School. Credit is given for this original activity to Mr. Buckley. Introduction "Micro"
Microscope Review. 1. A compound light microscope is represented in the diagram below.
Name Microscope Review Date 1. A compound light microscope is represented in the diagram below. 5. The diagram below represents a hydra as viewed with a compound light microscope. If the hydra moves toward
used for low power magnification of a sample image is 3 dimensional
MICROSCOPES One of the most important inventions in the advancement of Biology 1. Simple Microscopes ie. magnifying glass, stereoscope (dissecting scope) have a single lens or a pair of lenses combined
Anatomy: Introduction to the Light Microscope
Anatomy: Introduction to the Light Microscope Background: Microscopes are very important tools in biology. The term microscope can be translated as to view the tiny, because microscopes are used to study
Light Microscopy. Upon completion of this lecture, the student should be able to:
Light Light microscopy is based on the interaction of light and tissue components and can be used to study tissue features. Upon completion of this lecture, the student should be able to: 1- Explain the
Perfecting Microscope Skills
I. Introduction to the Microscope Perfecting Microscope Skills There are different types of microscopes used by biologists depending on the job they wish to accomplish, including dissecting (or "stereoscopic")
The light microscope
What is a microscope? The microscope is an essential tool in modern biology. It allows us to view structural details of organs, tissue, and cells not visible to the naked eye. The microscope should always
Marine Invertebrate Zoology Microscope Introduction
Marine Invertebrate Zoology Microscope Introduction Introduction A laboratory tool that has become almost synonymous with biology is the microscope. As an extension of your eyes, the microscope is one
BIOLOGY 1101 LAB 2: MICROSCOPES AND CELLS
BIOLOGY 1101 LAB 2: MICROSCOPES AND CELLS READING: Please read Chapter 4 in your text book to learn about the history of microscopy and basic cell structure. INTRODUCTION: The microscope is an important
LAB ACTIVITY: USING A MICROSCOPE
Name: Date: Period: Lab Partner(s): LAB ACTIVITY: USING A MICROSCOPE Objectives: Demonstrate the proper use and care of a compound light microscope and stereomicroscope. Focus the compound light microscope
STRUCTURE OF THE MICROSCOPE
STRUCTURE OF THE MICROSCOPE Use the word list to label the microscope below: Light Source Coarse adjustment knob Diaphragm Stage Clips Objectives Fine Adjustment Knob Base Stage Stage Clips Arm Revolving
Bio 252: Microscopy Study THE COMPOUND MICROSCOPE
Name: Date: Block: Microscope Number: Bio 252: Microscopy Study THE COMPOUND MICROSCOPE I. Introduction The compound microscope is one of the most important instruments used by biologists today. Through
Microscope (and The Cell) Lab Exercise #1
Lab Safety-General No eating or drinking Only registered students allowed in the class Long hair must be tied back Familiarize yourself with the emergency stations Do not mark on the models Inform me of
Basics of Light Microscopy and Metallography
ENGR45: Introduction to Materials Spring 2012 Laboratory 8 Basics of Light Microscopy and Metallography In this exercise you will: gain familiarity with the proper use of a research-grade light microscope
BASIC CULTURE TECHNIQUE: STREAK PLATE
Microbiology Laboratory (BIOL 3702L) Page 1 of 8 Principle and Purpose BASIC CULTURE TECHNIQUE: STREAK PLATE The isolation of pure cultures of microorganisms is a technique essential to many types of experiments
Microscope. Dr. Leena Barhate Department of Microbiology M.J.College, Jalgaon
Microscope Dr. Leena Barhate Department of Microbiology M.J.College, Jalgaon Acknowledgement http://www.cerebromente.org.br/n17/histor y/neurons1_i.htm Google Images http://science.howstuffworks.com/lightmicroscope1.htm
Microscope Skills. Scientific Skills the Microscope!
Microscope Skills Scientific Skills the Microscope! T. Trimpe 2005 http://sciencespot.net/ Body Tube Ocular lens (Eyepiece) Nosepiece Objectives Stage Clips Diaphragm Light Always carry a microscope with
Key Points Refer to How to Use the Compound Light Microscope :
MODULE 1 Objective 1.2 Lesson B Introduction to the Microscope Using the Light Microscope and Slide Preparation Course Advanced Biotechnology Unit Biotech Basics Essential Question How do scientists view
Using a Microscope. Year Group: BVSc1 + Document number: CSL_L07
Year Group: BVSc1 + Document number: CSL_L07 Equipment list: Equipment for this station: Microscope Power supply and a level surface to work on Gloves The sample to examine Marker or pencil for labelling
CALIBRATION OF MICROSCOPE EYEPIECE GRATICULE
CALIBRATION OF MICROSCOPE EYEPIECE GRATICULE A typical eyepiece graticule looks like this: It is 10mm in length and each mm is divided into 10 parts So each small division = 0.1mm = 100µm The eyepiece
Basic Microscopy for Plant Biology
Page 1 of 8 Basic Microscopy for Plant Biology OBJECTIVES After completing this exercise, you should be able to do the following: a. Name the parts of the compound microscope and the functions of each.
Introduction. Laboratory Equipment & Supplies. Model 1333PHi Shown (Phase Contrast) (2) Eyepieces (Eyecups installed) Diopter Adjustment Mechanism
Introduction With the invention of the microscope in the early 17th century, it was made possible to view objects which were too small for the human eye to see. As the microscope evolved, the structure
! Because microbiology deals with organisms too small they cannot be seen distinctly with the unaided eye, the microscope is essential.
Microscopy! Because microbiology deals with organisms too small they cannot be seen distinctly with the unaided eye, the microscope is essential.! The light microscope is the single most important research
The Microscope. Packet #2. 10/17/2016 9:12:02 PM Ryan Barrow 2012
1 The Microscope Packet #2 10/17/2016 9:12:02 PM Ryan Barrow 2012 2 Historical Timeline 1609 Galileo Galilei develops a compound microscope with a convex and a concave les. 1665 Robert Hooke publishes
Care and Use of the Compound Light Microscope
EXERCISE 2 Care and Use of the Compound Light Microscope Time Estimates for Completing This Lab The activities in this laboratory exercise can be completed in 2 to 2.5 hours. Extra time will be required
1.When an object is sharply focused and the slide is moved towards you, in which direction does the
image upright or inverted? Name: Date: _ BIOLOGY EXPERIMENT:Class: Using a Compound Light Microscope II: Depth Perception, resolution, field of view MATERIALS: Compound light microscopecolor magazine clipping
1. A laboratory technique is illustrated in the diagram below. Explain why the coverslip is lowered at an angle.
1. A laboratory technique is illustrated in the diagram below. Explain why the coverslip is lowered at an angle. 2. Base your answer to the following question on Which laboratory procedure is represented
Basic Microscopy. OBJECTIVES After completing this exercise, you should be able to do the following:
Page 1 of 10 Basic Microscopy OBJECTIVES After completing this exercise, you should be able to do the following: a. Name the parts of the compound microscope and the functions of each. b. Describe how
Using a Compound Light Microscope Lab Pre-Lab Assignment
Name: Block: Due Date: Using a Compound Light Microscope Lab Pre-Lab Assignment Pre-Lab Assignment This assignment must be completed by the next class period in order to be allowed to participate in the
MICROSCOPES. Magnification: Resolution: Field of View: Describes the visual picture seen when looking through the eyepiece of the microscope
Microscopes MICROSCOPES Magnification: Resolution: Field of View: Describes the visual picture seen when looking through the eyepiece of the microscope 7X 45X 112.5X 225X 1 st crude microscope made by
EXERCISE 3 The Microscope
Instant download and all chapters Solutions Manual Human Anatomy Laboratory Manual with Cat Dissections 7th Edition Marieb Smith https://testbankdata.com/download/solutions-manual-human-anatomy-laboratorymanual-cat-dissections-7th-edition-marieb-smith/
Microscopes & cells. 2. arm. 3. ocular lens. 4. objective lenses. 5. stage. 6. slide clamp. 7. stage controls
Microscopes & cells Objectives: At the end of this lab you should be able to: o demonstrate the safe and proper handling of a microscope, including carrying a microscope, slide placement, and storage.
Laboratory 2: Microscopy and Observation of Cells authors: Dr. Ruth Dahlquist-Willard & Michael Kunz
Laboratory 2: Microscopy and Observation of Cells authors: Dr. Ruth Dahlquist-Willard & Michael Kunz Corresponding Readings: Campbell Ch. 4 BIOL-100L Safety Information: We will be using laboratory glassware
The invention of the microscope made it possible for scientists to view and study cells. Cells the basic units of all living organisms.
The Discovery of Cells The invention of the microscope made it possible for scientists to view and study cells. Cells the basic units of all living organisms. The Cell Theory All living things are made
Lab: The Compound Microscope
Lab: The Compound Microscope Purpose: To learn the parts of the compound microscope and to learn the basic skills needed to use the microscope properly. Materials: Microscope Colored paper Cover slips
MICROSCOPE TERMS 7X 45X 112.5X 225X
Microscopes MICROSCOPE TERMS Magnification- how much larger the image is Resolution- how clear the image is Field of View: Describes the visual picture seen when looking through the eyepiece of the microscope
Ocular Lenses. Head. Arm. Objective Lenses. Slide Holder Stage. On / Off Switch. Condenser with Iris Diaphragm. Light Intensity Control
BIOLOGY 211: HUMAN ANATOMY & PHYSIOLOGY ********************************************************************************************************* USE OF THE LIGHT MICROSCOPE **********************************************************************************************************
Introduction to Microscopes
INTRODUCTION TO THE MICROSCOPE Introduction to Microscopes The first microscopes worked by the same basic principle as the ones you will be using in lab. They are light microscopes. Visible light passes
Microscopy. Danil Hammoudi.MD
Microscopy Danil Hammoudi.MD Care and Handling of the Microscope: A microscope is a delicate piece of equipment and should be treated with care. Use two hands when carrying the microscope. Place one hand
Motorized Axio Observer Start-up instructions
Start-up instructions 1. If using fluorescence turn on Fluorescent light source. TL light Source (Hal 100) 2. Turn on microscope using switch on lower left side of the microscope. 3. If imaging, turn on
Biology The Microscope. May 20 1:19 PM. Using a Microscope to Explore the Cell
Biology 2201 1.2 The Microscope Using a Microscope to Explore the Cell Resolution or Resolving power The ability of the eye, or other instrument, to distinguish between two objects that are close together
Indian Institute of technology Madras Presents NPTEL NATIONAL PROGRAMME ON TECHNOLOGY ENHANCED LEARNING
Indian Institute of technology Madras Presents NPTEL NATIONAL PROGRAMME ON TECHNOLOGY ENHANCED LEARNING Lecture - 5 Materials Characterization Fundamentals of Optical microscopy Dr. S. Sankaran Associate
I. The First Microscopes. Microscope Basics. II. The Bright Field Microscope. Confocal Laser Scanning Microscopy. A. The Compound Microscope
Microscope Basics I. The First Microscopes NGSSS: SC.912.N.2.1 through N.4.2 A. About 1590, two Dutch spectacle makers, Zaccharias Janssen and his son Hans, while experimenting with several lenses in a
Tissue Preparation ORGANISM IMAGE TISSUE PREPARATION. 1) Fixation: halts cell metabolism, preserves cell/tissue structure
Lab starts this week! ANNOUNCEMENTS - Tuesday or Wednesday 1:25 ISB 264 - Read Lab 1: Microscopy and Imaging (see Web Page) - Getting started on Lab Group project - Organ for investigation - Lab project
Microscopy Primer. Fig A compound light microscope with important parts labeled.
BIOL 221 Concepts of Botany Fall 2010 Microscopy Primer A. Introduction: The microscope is a vital scientific tool that will be used often to study plants. We shall begin our studies of plants with a brief
Instruction Manual T Binocular Acromat Research Scope T Trinocular Acromat Research Scope
Research Scope Instruction Manual T-29031 Binocular Acromat Research Scope T-29041 Trinocular Acromat Research Scope T-29032 Binocular Semi-Plan Research Scope T-29042 Trinocular Semi-Plan Research Scope
Using Microscopes. Life Science: Molecular
Using Microscopes Life Science: Molecular Light Microscopy: Instrumentation and Principles A light microscope is so named because it uses visible light to produce a magnified image. Compound light microscopes
Imaging Introduction. September 24, 2010
Imaging Introduction September 24, 2010 What is a microscope? Merriam-Webster: an optical instrument consisting of a lens or combination of lenses for making enlarged images of minute objects; especially:
UNIT: THE MICROSCOPE AND CELLULAR DIVERSITY
Course: Biology Agricultural Science & Technology UNIT: THE MICROSCOPE AND CELLULAR DIVERSITY State Standard: State Objectives: Unit Objectives: A. Learn how to use the compound microscope. B. Learn the
FLUORESCENCE MICROSCOPY. Matyas Molnar and Dirk Pacholsky
FLUORESCENCE MICROSCOPY Matyas Molnar and Dirk Pacholsky 1 The human eye perceives app. 400-700 nm; best at around 500 nm (green) Has a general resolution down to150-300 μm (human hair: 40-250 μm) We need
Lab: Using a Compound Light Microscope
Name Date Period Lab: Using a Compound Light Microscope Background: Microscopes are very important tools in biology. The term microscope can be translated as to view the tiny, because microscopes are used
VISUAL PHYSICS ONLINE DEPTH STUDY: ELECTRON MICROSCOPES
VISUAL PHYSICS ONLINE DEPTH STUDY: ELECTRON MICROSCOPES Shortly after the experimental confirmation of the wave properties of the electron, it was suggested that the electron could be used to examine objects
Title: Thinking with the Eyes Author(s): Elizabeth Haggerty Hutton Date Created: 8/5/2011 Subject: Biology Grade Level: 9 th Grade Honors Standards:
Title: Thinking with the Eyes Author(s): Elizabeth Haggerty Hutton Date Created: 8/5/2011 Subject: Biology Grade Level: 9 th Grade Honors Standards: SC.912.N.1.1: The practice of science SC.912.L.14.4:
microscopy A great online resource Molecular Expressions, a Microscope Primer Partha Roy
Fundamentals of optical microscopy A great online resource Molecular Expressions, a Microscope Primer http://micro.magnet.fsu.edu/primer/index.html Partha Roy 1 Why microscopy Topics Functions of a microscope
Very short introduction to light microscopy and digital imaging
Very short introduction to light microscopy and digital imaging Hernan G. Garcia August 1, 2005 1 Light Microscopy Basics In this section we will briefly describe the basic principles of operation and
What is it? Study the mystery photos and try to identify each one! Have access to a computer?
Station 1 Solve the Mystery What is it? Study the mystery photos and try to identify each one! They are all common objects that might be found in your home or a classroom. Write your guesses for the mystery
REVIEW FOR TEST ON MONDAY
1. The diagram below shows an ameba moving out of the high-power field of view of a compound microscope in the direction indicated by the arrow. 4. The diagram below represents two cells next to a metric