Jeffrey J. Field, Ph.D.
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1 Jeffrey J. Field, Ph.D. Director, Microscope Imaging Network Core Facility Assistant Professor, Department of Biochemistry and Molecular Biology Research Scientist I, Department of Electrical and Computer Engineering office: (970) lab: (970) www: min.colostate.edu 1301 Campus Delivery Fort Collins, CO RESEARCH INTERESTS Coherent diffractive imaging; novel non-luminescent contrast mechanisms for biological microscopy; highfidelity imaging through turbid media; novel multiphoton microscopy imaging platforms; super-resolution microscopy; ultrafast optics; nonlinear optics; computational imaging; computational biophysics. EDUCATION Colorado School of Mines (CSM), Golden, CO Ph.D. in Applied Physics with Dr. Jeff A. Squier ( ) Thesis title: Differential Multiphoton Microscopy M.Sc. in Applied Physics with Dr. Jeff A. Squier ( ) Thesis title: High-efficiency ultrashort pulse shaper using a 4096-element reflective spatial light modulator B.Sc. in Engineering Physics ( ) ACADEMIC EMPLOYMENT Oct present Assistant Professor Dept. of Biochemistry and Molecular Biology (Non-tenure track) Jan present Director Microscope Imaging Network Core Facility, Dept. of Biochemistry and Molecular Biology Oct present Research Scientist I Dept. of Electrical and Computer Engineering Aug Dec Interim Director Microscope Imaging Network Core Facility, Dept. of Biochemistry and Molecular Biology Feb Sep Postdoctoral Fellow Dept. of Electrical and Computer Engineering Mar Feb Postdoctoral Fellow Department of Physics University of California, San Diego May 2010 Feb Postdoctoral Fellow Department of Physics 1
2 Colorado School of Mines Aug May 2010 Graduate Research Assistant Department of Physics Colorado School of Mines HONORS AND AWARDS 2013 Best poster presentation by a postdoc, Front Range Neuroscience Group Annual Meeting 2012 National Research Council Postdoctoral Fellowship (Did not accept) National Institute of Standards and Technology, Boulder, CO, Time-Frequency Division Awards determined by a competitive grant application process Proposal was written for work in Dr. Scott Diddams' group 2006 Runner-up, Best Poster Award, Colorado School of Mines Graduate Research Fair TEACHING EXPERIENCE Spring 2017 (scheduled) Guest Lecturer & Lab Coordinator Biology CM702E Methods in Cell and Molecular Fall 2016 Guest Lecturer ECE581B7/BIOM581B7 Advanced Optical Imaging Spring 2015 Guest Lecturer ECE457 Fourier Optics Spring 2007 Graduate Teaching Assistant Graduate Quantum Mechanics II Spring 2006 Graduate Teaching Assistant Quantum Optics Graduate Teaching Assistant Physics I: Mechanics Summer 2005 Graduate Teaching Assistant Physics Field Session, Computing Section PUBLICATIONS Peer-Reviewed Journal Articles J.J. Field, K.A. Wernsing, J.A. Squier, and R.A. Bartels, Toward deep-tissue super-resolution microscopy, Opt. Photon. News, Year in Review Special Issue, (Dec. 2016). In press. J.J. Field, D.G. Winters, and R.A. Bartels, "Single-pixel fluorescent imaging with temporally labeled illumination patterns," Optica 3, (2016). J.J. Field, K.W. Wernsing, S.R. Domingue, A.M. Allende-Motz, Keith F. DeLuca, Dean H. Levi, Jennifer G. DeLuca, Michael D. Young, Jeff A. Squier and Randy A. Bartels, "Superresolved multiphoton microscopy with spatial frequency-modulated imaging," Proc. Nat. Acad. Sci. USA 113, (2016). E. Block, M.D. Young, D.G. Winters, J.J. Field, R.A. Bartels and J.A. Squier, "Simultaneous spatial frequency modulation imaging and micromachining with a femtosecond laser," Opt. Lett. 41, (2016). J.J. Field, D.G. Winters, and R.A. Bartels, "Plane wave analysis of coherent holographic image reconstruction by phase transfer (CHIRPT)," J. Opt. Soc. Am. A. 32, (2015). M.D. Young, J.J. Field, K.E. Sheetz, R.A. Bartels, and J.A. Squier, A pragmatic guide to multiphoton microscope design, Invited tutorial, Adv. Opt. Photon. 7, (2015). 2
3 P.S. Tsai, C. Mateo, J.J. Field, C.B. Schaffer, M.E. Anderson, and D. Kleinfeld, "Ultra-large field-of-view twophoton microscopy," Opt. Express 23, (2015). E.E. Hoover, J.J. Field, D.G. Winters, M.D. Young, E.V. Chandler, J.C. Speirs, J.T. Lapenna, S.M. Kim, S.-y. Ding, R.A. Bartels, J.W. Wang, and J.A. Squier. Eliminating the scattering ambiguity in multifocal, multimodal, multiphoton imaging systems, Invited article, J. Biophotonics, 5, (2012). J.J. Field, K.E. Sheetz, E.V. Chandler, E.E. Hoover, M.D. Young, S.-Y. Ding, A. Sylvester, D. Kleinfeld, and J.A. Squier. Differential multiphoton laser scanning microscopy, Invited article, IEEE J. Sel. Top. Quant. Elec. 18, (2012). J.D. Driscoll, A.Y. Shih, J.J. Field, G.A. White, J.A. Squier, G. Cauwenberghs, and David Kleinfeld, Photon counting and fluorescent lifetime imaging for improved detection in two-photon microscopy, J. Neurophys. 105, (2011). E.E. Hoover, M.D. Young, E.V. Chandler, A. Luo, J.J. Field, K.E. Sheetz, A.W. Sylvester, and J.A. Squier, Remote focusing for programmable multi-layer differential multiphoton microscopy, Biomed. Opt. Exp. 2, (2011). J.J. Field, C.G. Durfee, and J.A. Squier, Blind FROG measurements for quantitative differential multiphoton microscopy, Opt. Lett. 35, (2010). J.J. Field, R. Carriles, K. Sheetz, E. Hoover, E. Chandler, S. Tillo, T. Hughes, A. Sylvester, D. Kleinfeld, and J.A. Squier, Optimizing the fluorescent yield in two-photon laser scanning microscopy with dispersion compensation, Opt. Exp. 18, (2010). R. Carriles, D.N. Schafer, K.E. Sheetz, J.J. Field, R. Cisek, V. Barzda, and J.A. Squier, Invited Review Article: Imaging techniques for harmonic and multiphoton absorption fluorescence microscopy, Invited article, Rev. Sci. Instrum. 80, (2009). E.V. Chandler, E.E. Hoover, J.J. Field, K.E. Sheetz, W.A. Amir, R. Carriles, S. Ding, and J.A. Squier, Highresolution mosaic imaging with multifocal, multiphoton photon-counting microscopy, App. Opt. 48, (2009). J.J. Field, C.G. Durfee, J.A. Squier, and S. Kane, Quartic-phase-limited grism-based ultrashort pulse shaper, Opt. Lett. 32, (2007). J.J. Field, T.A. Planchon, W. Amir, C.G. Durfee, and J.A. Squier, Characterization of a high efficiency, ultrashort pulse shaper incorporating a reflective 4096-element spatial light modulator, Opt. Comm. 278, (2007). T.A. Planchon, W. Amir, J.J. Field, C.G. Durfee, J.A. Squier, P. Rousseau, O. Albert, and G. Mourou, Adaptive correction of a tightly focused, high-intensity laser beam by use of a third-harmonic signal generated at an interface, Opt. Lett. 31, (2006). Book Chapter E.E. Hoover, E.V. Chandler, J.J. Field, D.N. Vitek, M.D. Young, and J.A. Squier. Utilising ultrafast lasers for multiphoton biomedical imaging. In Ultrafast Nonlinear Optics. Springer International Publishing, Conference Proceedings J.J. Field and R.A. Bartels, Digital aberration correction of fluorescent images with coherent holographic image reconstruction by phase transfer (CHIRPT), Proc. SPIE 9317, 97130B (2016). K.A. Wernsing, J.J. Field, S.R. Domingue, A.M. Allende-Motz, K.F. DeLuca, D.H. Levi, J.G. DeLuca, M.D. Young, J.A. Squier and R.A. Bartels, Point spread function engineering with multiphoton SPIFI, Proc. SPIE 9713, (2016). 3
4 Technical Whitepapers & Application Notes Shaping ultrafast optical pulses with liquid crystal spatial light modulators, Boulder Nonlinear Systems Application Note 8010, May Available online: (Last accessed: ) CONFERENCE CONTRIBUTIONS J. J. Field and R. Bartels, "Digital refocusing of fluorescent light intensity with spatial frequency modulated imaging," in Frontiers in Optics 2016, OSA Technical Digest (online) (Optical Society of America, 2016), paper FTh4C.7. P.A Stockton, R.A. Bartles, and J.J. Field, Simultaneous fluorescent and quantitative phase imaging through spatial frequency projections, in Frontiers in Optics 2016, OSA Technical Digest (online) (Optical Society of America, 2016), paper FTu2F.2. K.A. Wernsing, J. J. Field, and R.A. Bartels, "Super-Resolved Microscopy with Spatial Frequency-Modulated Imaging," in Frontiers in Optics 2016, OSA Technical Digest (online) (Optical Society of America, 2016), paper JW4A.121. M. D. Young, J. J. Field, R. Bartels, and J. Squier, Spatial Frequency Modulated Imaging (SPIFI) in Amplitude with a Spatial Light Modulator," in Frontiers in Optics 2016, OSA Technical Digest (online) (Optical Society of America, 2016), paper JTh2A.57. R. Stokoe, P. Stockton, J.J. Field, and R.A. Bartels, Large field of view single pixel interference projection imaging, Biomedical Engineering Society Annual Meeting, Minneapolis MN, M.D. Young, E.K. Block, D.G. Winters, J.J. Field, K.A. Wernsing, R.A. Bartels and J.A. Squier, Simultaneous spatial frequency modulation imaging and micromachining with a femtosecond laser, Proc. SPIE 9764, (Feb. 16, 2016). K.A. Wernsing, J.J. Field, S.R. Domingue, A.M. Allende-Motz, K.F. DeLuca, D.H. Levi, J.G. DeLuca, M.D. Young, J.A. Squier and R.A. Bartels, Super-resolved nonlinear microscopy with spatial frequency modulated imaging, Proc. SPIE 9713, (Feb. 15, 2016) J.J. Field, D.G. Winters and R.A. Bartels, Coherent image reconstruction with incoherent contrast light, Proc. SPIE 9713, (Feb. 15, 2016) R. A. Bartels and J.J. Field, "Single Pixel Tomographic Fluorescent Imaging with Spatial-temporal Modulated Illumination Light," in Optics in the Life Sciences, OSA Technical Digest (online) (Optical Society of America, 2015), paper NW2C.1. J.A. Squier, E. Block, M. Greco, M. Young, C.G. Durfee, J. Thomas, J.J. Field, and R. Bartels, "Multiphoton imaging and manipulation of biological systems," in CLEO: 2014, OSA Technical Digest (Optical Society of America, 2014), paper ATu3P.2. J.J. Field, M.D. Young, C.M. Eitel, S.A. Tobet, J.A. Squier, and R.A. Bartels, A large field-of-view nonlinear microscope for biological imaging, 11th Annual Meeting of the Front Range Neuroscience Group, Fort Collins, CO, Dec P.S. Tsai, M. Anderson, J.J. Field, C. Mateo, and D. Kleinfeld, Ultrawide-field two photon laser scanning microscope, Society for Neuroscience Annual Meeting (November, 2013). J.J. Field, High-speed volumetric fluorescence and nonlinear microscopy through phase-encoded axial position, SPIE Ultrafast Imaging and Spectroscopy, Paper , San Diego, CA, Aug J.J. Field, C. Eitel, D. Smith, S. Tobet, and Randy Bartels, A Survey of Harmonic Generation in Murine Tissues under Multimodal Multiphoton Microscopy, Biomedical Engineering Society Annual Meeting, Atlanta GA, R. Carriles, E. E. Hoover, J.J. Field, K. E. Sheetz, and J.A. Squier, Multifocal nonlinear microscope with single element detector for multidepth imaging, Proc. SPIE 8011, 22nd Congress of the International Commission for Optics: Light for the Development of the World, 80118K (Nov. 2, 2011). 4
5 E. E. Hoover, M. D. Young, S. M. Kim, E. V. Chandler, J.J. Field, D. N. Vitek, K. E. Sheetz, J. W. Wang, and J. A. Squier, Remote focusing differential multiphoton microscopy: Application to neuronal imaging, CLEO Presentation CThF, Baltimore, MD (2011). A. Meier, J.J. Field, S. Kane, J. Squier, C. Durfee, Design of a 3-bounce grism compressor for ultrafast laser pulses, APS March Meeting, D (2010). J.A. Squier, J.J. Field, E.E. Hoover, E.V. Chandler, M.D. Young, and D.N. Vitek, Differential Multiphoton Microscopy, in International Conference on Ultrafast Phenomena, OSA Technical Digest (Optical Society of America, 2010), paper WC6. J.J. Field, C.G. Durfee, and J. Squier, Blind FROG measurements for quantitative differential multiphoton microscopy, CLEO Presentation CMK2, San Jose, CA (2010). M. Young, E. Hoover, E. Chandler, J.J. Field, and J. Squier, Axial-scanning techniques for multiphoton imaging, Four Corners Section of APS Meeting, Golden, CO (2009). E. Hoover, E. Chandler, J.J. Field, K. Sheetz, R. Carriles, and J. Squier, Using FPGAs for simultaneous photon counting imaging of multiple focal planes, Four Corners Section of APS Meeting, Golden, CO (2009). E.V. Chandler, E.E. Hoover, J.J. Field, K. Sheetz, R. Carriles, and J. Squier, Simultaneous Imaging of Multiple Focal Planes via Multiphoton, Photon-Counting Microscopy, Novel Techniques in Microscopy, OSA Technical Digest (CD) (Optical Society of America, 2009), paper NMD4. J.J. Field, R. Carriles, E. Chandler, E. Hoover, K. Sheetz, and J. Squier, Multimodal, multifocal, multiphoton, photon-counting microscopy, Novel Optical Materials and Applications Conference, Cetraro, Italy (2009). Invited talk. J. Squier, W. Amir, R. Carriles, E. Chandler, J.J. Field, E. Hoover, D. Schafer, and K. Sheetz, Multifocal, multimodal, photon counting, multiphoton microscopy, CLEO Presentation CME1, Baltimore, MD (2009). Invited talk. J.J. Field, R. Carriles, J. Squier, and D. Kleinfeld, Photon-counting photobleaching measurements and the effect of dispersion in two-photon microscopy, CLEO Poster Session JTuD58, Baltimore, MD (2009). R. Carriles, J.J. Field, E. Hoover, K. Sheetz, and J. Squier, Multimodal, multifocal, multiphoton, photoncounting microscopy, Photonics West, BiOS Session [ ], (2009). C.G. Durfee, J.A. Squier, J.J. Field, and S. Kane, A Modular Approach to Spectral Phase Calculation: Application to Grism Optimization, Frontiers in Optics, OSA Technical Digest (CD) (Optical Society of America, 2008), paper FWQ4. C. Durfee, J.J. Field, J. Squier, and S. Kane, Control of Spectral Phase of Ultrafast Optical Pulses with Grisms, 2008 Joint Fall Meeting of the Texas and Four Corners Sections of APS, AAPT, and Zones 13 and 16 of SPS, and the Societies of Hispanic and Black Physicists, Oct. 2008, abstract #CFC.40. J.J. Field, C. Durfee, S. Kane, and J. Squier, Quartic-phase-limited grism-based femtosecond pulse shaper, CLEO Presentation CThDD2, San Jose, CA (2008). C. Durfee, T. Planchon, S. Bera, J.J. Field, D. Adams, C. Childress, and J. Squier, Development of a khzrepetition rate TW ultrafast amplifier, Four Corners Section of the APS Fall Meeting, Oct. 2006, abstract #B J.J. Field, T.A. Planchon, C.G. Durfee, and J.A. Squier, High resolution and high efficiency prism-based pulse shaper, Colorado Photonics Industry Association Annual Meeting Poster Session, Boulder, CO (2006). T.A. Planchon, W. Amir, J.J. Field, P. Rousseau, O. Albert, G. Mourou, C.G. Durfee, and J. Squier, Simple and robust solution for high-intensity laser adaptive optic correction on a tightly focused beam using thirdharmonic signal generated at an interface, CLEO Poster Session JTuD, Long Beach, CA (2006). J.J. Field, T.A. Planchon, C.G. Durfee, and J.A. Squier, High resolution and high efficiency prism-based pulse shaper, CLEO Poster Session JWB41, Long Beach, CA (2006). 5
6 INVITED SEMINAR TALKS Developing next-generation microscopes for biological imaging, Department of Biochemistry and Molecular Biology Seminar Talk,. Presentation date: Sep. 19, "New Tools for Biological Imaging," Department of Physics Colloquium Talk, Colorado School of Mines. Presentation date: Jan. 27, New Tools for Large Scale Biological Imaging with High Spatial Resolution, School of Biomedical Engineering Seminar Talk,. Presentation date: April 7, References available upon request. 6
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