Prep TM Automated Extraction and Clean-up System for PCDDs, PCDFs and PCBs
|
|
|
- Phebe West
- 6 years ago
- Views:
Transcription
1 Power-Prep Prep TM Automated Extraction and Clean-up System for PCDDs, PCDFs and PCBs Jef Focant and Hamid Shirkhan Dioxin Lab. BELGIUM FMS Inc. USA
2 Dioxins Analysis! Extraction (Soxhlet, L-L, Micro-waves, SFE, ASE, SPE, )! Clean-up (Liquid chromatography)! Analysis (GC/MS, bio-assays, )
3 Power-prep System! Automated sample prep. System! PC controlled valves and pump! Modular system (1 to 20 samples in )! Stalling et al. paper based (Si, Al, C)! Disposable columns (1-25g packing)
4 Valves Driver Pump and Pressure Power supply Controller Valves
5 Gauge Silica 1 2 M3 1 2 M3 M2 Pump Hexane 2. 2% DCM 3. 50% DCM 4. EtAc/Benz 5. Toluene 1 2 M4 Carbon Sample Alumina 1 2 M4 M M6 2 M M M7 M5 1 2 M8 1. PCDD/Fs 2. PCB 3. Fraction 1 4. Fraction 2 5. Fraction 3 Organic waste
6 Multi-Matrix Matrix System! Biological (Patterson Jr., CDC)! Environmental (Rivera, Spain)! Food (De Pauw, Belgium)
7 PCDD/Fs and PCBs in Foodstuff! 95% of intake is via food! Dioxins are located in fat! Norm PCDD/Fs = 5 pg I-TEQ/g fat! Norm PCBs = 200 ng/g fat
8 Solid Samples (Meat, fish, dairy fat, eggs, )
9 Lipids Isolation! Sample Homogenisation (liquid N ) 2! Freeze-drying (overnight)! Grinding! ASE extraction (overnight)! Lipids determination and spiking
10 Background Levels Matrix Pork Horse Beef Poultry Eggs (yolk) Levels pg I-TEQ/g I fat
11 Required Amount of Fat Very low level of contamination ( 0.1 ppt) + LOQ (GC/IDHRMS( GC/IDHRMS) 0.1 ppt and recoveries <100% More than 1g fat is needed 4-55 g lipids are usually necessary
12 Clean-up (Fat Removal)! GPC is ϑ versatile and robust Λ a time limiting step!!! Power-Prep Prep is ϑ automated and rapid Λ limited to 1 g of fat... High Capacity Disposable Silica Columns (HCDS)
13 High Capacity Disposable Silica Columns (HCDS) Goal : GPC Increase the sample throughput Simplify the clean-up Properties : 28g acidic,, 16g basic, 6g neutral Disposable Part of PwP step
14 Si Al C HCDS
15 1,2,3,6,7,8-HxCDD 1,2,3,7,8,9-HxCDD 1,2,3,4,6,7,8-HpCDD OCDD 2,3,7,8-TCDF 1,2,3,7,8-PeCDF 2,3,4,7,8-PeCDF 1,2,3,4,7,8-HxCDF 1,2,3,6,7,8-HxCDF 1,2,3,7,8,9-HxCDF 2,3,4,6,7,8-HxCDF 1,2,3,4,6,7,8-HpCDF OCDF 3,3',4,4'-TCB 3,3',4,4',5-PeCB 3,3',4,4',5,5'-HxCB 1,2,3,4,7,8-HxCDD 2,3,7,8-TCDD 1,2,3,7,8-PeCDD % Recoveries 0 Recoveries for QC HCDS GPC
16 QC Chart for «in-house QC» 5,2 GPC HCDS Concentration (ppt) 4,7 4,2 3,7 3, Run number
17 Quality Control HCDS GPC RSD (%) Trueness (%) Range [4-16] [88-107] Average Range [3-11] [87-101] Average 6 96! Same blank levels! No carry-over! No GC/MS differences
18 HCDS vs GPC for Eggs HCDS GPC 1 TCDD PeCDD HxCDD 1 HxCDD 2 HxCDD 3 HpCDD OCDD TCDF PeCDF 1 PeCDF 2 HxCDF 1 HxCDF 2 HxCDF 3 HxCDF 4 HpCDF1 OCDF PCB 77 PCB 126 PCB pgteq/g FAT
19 Liquid Samples (Milk, digested tissues, )
20 Upgrade to Extraction! The system is implemented to include online extraction prior to clean-up! SPE on C is 18 carried out! Additional valves and solvents (aqueous)! Drying under N pressure 2! Sample FAST clean extract
21 Sample pre-treatment! Milk reconstitution (powder sample) or digestion of sample (adipose)! Spike! Fat globules coalescence! Side lipid percent determination
22 Gauge M6 M3 Pump M2 1. Hexane 2. 2% DCM 3. 50% DCM 4. EtAc/Benz 5. Toluene C M3 M4 Nitrogen M1 Carbon Sample 1. Water 2. Acetonitrile 3. 50% DCM Air Aques waste 1 2 M4 M6 1 2 M6 1 2 Silica Alumina M4 M5 M5 M M6 2 1 M6 1 M7 1 2 M8 1. PCDD/Fs 2. PCB 3. Fraction 1 4. Fraction 2 5. Fraction 3 Organic waste
23 SPE
24 Real Milk Samples! Background : ~ pg TEQ/g fat! 40 ml full fat (3.6 %) 1.5 g lipids! Sample load volume ~ 150 ml! Drying 30 min./30 PSI N 2! Hexane elute C Classical 18 run
25 Recoveries for Milk SPE-PWP FLASH SPE 1,2,3,4,7,8-HxCDD 1,2,3,6,7,8-HxCDD 1,2,3,7,8,9-HxCDD 1,2,3,4,6,7,8-HpCDD OCDD 2,3,7,8-TCDF 1,2,3,7,8-PeCDF 2,3,4,7,8-PeCDF 1,2,3,4,7,8-HxCDF 1,2,3,6,7,8-HxCDF 1,2,3,7,8,9-HxCDF 2,3,4,6,7,8-HxCDF 1,2,3,4,6,7,8-HpCDF OCDF 3,3',4,4'-TCB 3,4,5,4'-TCB 3,3',4,4',5-PeCB 3,3',4,4',5,5'-HxCB 1,2,3,7,8-PeCDD 2,3,7,8-TCDD % Recoveries
26 Reference Material (RM ( 534) 4,00 3,50 Assigned Measured 3,00 2,50 2,00 1,50 1,00 0,50 0,00 2,3,7,8-TCDD 1,2,3,7,8-PeCDD 1,2,3,4,7,8-HxCDD 1,2,3,6,7,8-HxCDD 1,2,3,7,8,9-HxCDD 2,3,7,8-TCDF 1,2,3,7,8-PeCDF 2,3,4,7,8-PeCDF 1,2,3,4,7,8-HxCDF 1,2,3,6,7,8-HxCDF 2,3,4,6,7,8-HxCDF ~ 4 g reconstituted material Mean RSD ~ 5% pg/g powder
27 Certified Material (CRM( 607) BCR 607 Assigned Values Measured Values Conc. pg/g SD pg/g Mean SD pg/g RSD % Justness % 2,3,7,8 TCDD 0,25 0,03 0,34 0, ,2,3,7,8 PeCDD 0,79 0,04 1,03 0, ,2,3,4,7,8 HxCDD 0,42 0,07 0,40 0, ,2,3,6,7,8 HxCDD 0,98 0,11 0,98 0, ,2,3,7,8,9 HxCDD 0,34 0,05 0,37 0, ,3,7,8 TCDF 0,05 0,03 n.d. 1,2,3,7,8 PeCDF 0,05 0,01 n.d. 2,3,4,7,8 PeCDF 1,81 0,13 2,23 0, ,2,3,4,7,8 HxCDF 0,94 0,04 0,81 0, ,2,3,6,7,8 HxCDF 1,01 0,09 1,10 0, ,3,4,6,7,8 HxCDF 1,07 0,05 1,18 0, Total 7,71 0,23 8,43 0, ~ 8 g reconstituted material
28 2,5 2 1,5 1 0,5 0 Manual vs Automated SPE-PWP FLASH SPE 1,2,3,7,8-PeCDD 1,2,3,4,7,8-HxCDD 1,2,3,6,7,8-HxCDD 1,2,3,7,8,9-HxCDD 2,3,7,8-TCDF 1,2,3,7,8-PeCDF 2,3,4,7,8-PeCDF 1,2,3,4,7,8-HxCDF 1,2,3,6,7,8-HxCDF 2,3,4,6,7,8-HxCDF 2,3,7,8-TCDD ~ 8 g reconstituted CRM 607 pg/g powder
29 General Situation Fast Sampling 1 h Fast Extraction & clean-up 2 h Slow Concentration 15 h Fast GC/MS analysis 1 h
30 System Evaluation! Repeatability (Acceptable RSD s)! Reproducibility (over time and personnel)! Robustness Solids : Eggs, pork, beef, poultry, mackerel, sperm whale, dairy fat, ) Liquids : Milk, serum (CDC),...
31 Conclusions! Automated extraction AND clean-up! Versatility (matrix, analytes)! Increased sample throughput! Reduced solvent consumption! Short personnel training
32 Acknowledgments! All the lab. staff! F.R.I.A. for financial support! FMS Inc. for collaboration! Thermo Finnigan MAT for symposium
A new gold standard for Dioxin analysis A combined approach ; automated efficient clean-up together with a new Triple Quadrupole GC-MS/MS
A new gold standard for Dioxin analysis A combined approach ; automated efficient clean-up together with a new Triple Quadrupole GC-MS/MS Haruhiko Miyagawa, GCMS Product Manager Analytical &Measuring Instruments
RSR011 SEVERN TRENT LABORATORIES, INC. Run Date: 9/22/06 SAMPLE CONFIRMATION REPORT Page : 1 Time : 8:06:14 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - The following
Persistant Organic Pollutants
Persistant Organic Pollutants Fast screening and precise confirmation with GC-MSMS and GC-HRMS. Hans-Joachim Huebschmann GC-MS Technology Manager Dirk Krumwiede Product & Application Specialist for Advanced
Minnesota Department of Health Environmental Laboratory Accreditation Program
Minnesota Department of Health Environmental Laboratory Accreditation Program Issues accreditation to State Laboratory ID: 048-999-468 EPA Lab Code: 02693 ALS Group USA Corp dba ALS Environmental Houston
Results from UNEP 2 nd Interlaboratory
Results from UNEP 2 nd Interlaboratory Overview on the intercalibration data of dl-pops Van Bavel B 1, Van der Veen I 2, Nilsson H 1, De Boer J 2, Fiedler H 3 1 Örebro University, School of Science and
Improved Separation and Quantification of Dioxin and Furan Congeners Using an Application-Specific Capillary Gas Chromatographic Column and GC-HRMS
Improved Separation and Quantification of Dioxin and Furan Congeners Using an Application-Specific Capillary Gas Chromatographic Column and GC-HRMS Frank Dorman, Shawn Reese, Gary Stidsen Restek Corporation,
Date Issued: July 01, 2018 Expiration Date: June 30, 2019
State of Florida Department of Health, Bureau of Public Health Laboratories This is to certify that VISTA ANALYTICAL LABORATORY INC. 1104 WINDFIELD WAY EL DORADO HILLS, CA 95762 has complied with Florida
Site-specific Bioaccumulation of PCDD/PCDFs in Mothers and their Infants Living in Vicinity of Bien Hoa Airbase, Southern Vietnam
4 th International Forum on Sustainable Future in Asia 4 th NIES International Forum Site-specific Bioaccumulation of PCDD/PCDFs in Mothers and their Infants Living in Vicinity of Bien Hoa Airbase, Southern
EVALUATION OF PCDDs AND PCDFs IN WILD GAME TAKEN FROM THE FLOODPLAIN ALONG THE TITTABAWASSEE RIVER
EVALUATION OF PCDDs AND PCDFs IN WILD GAME TAKEN FROM THE FLOODPLAIN ALONG THE TITTABAWASSEE RIVER Prepared by: ENTRIX, Inc. East Lansing, Michigan Prepared for: The Dow Chemical Company Midland, Michigan
Approaches to highest sensitivity analysis of selected Dioxin / Furan congeners:
Approaches to highest sensitivity analysis of selected Dioxin / Furan congeners: a.) Cryogenic Peak Zone Focusing (cyro - PZF) b.) Fast GC Dirk Krumwiede, Jeff Focant, Heinz Mehlmann, Daniela Cavagnino,
Photograph: Norith Eckbo
Photograph: Norith Eckbo Contaminants in Polar Regions PBDEs Particle deposition (high K OA ) HCHs High volatility: Single hoppers (high K AW ) Condensation DDT PCBs Evaporation Low volatility (low K AW
Profiling of Volatile Organic Compounds in Milk and Orange Juice Using Headspace Analysis
Profiling of Volatile Organic Compounds in Milk and Orange Juice Using Headspace Analysis HT3 Application Note Introduction Volatile organic compounds, VOCs, present in liquid food products play a very
Thermo Scientific DFS
Thermo Scientific DFS High Resolution Sector Field MS Instrument performance standards for Dioxin / Furan analysis Dirk Krumwiede, Product Specialist Trace MS Overview: presentation main topics 1.) NEW
Agilent 7697A GC Headspace Sampler YOUR GENUINELY BETTER SOLUTION FOR VOLATILES ANALYSIS
Agilent 7697A GC Headspace Sampler YOUR GENUINELY BETTER SOLUTION FOR VOLATILES ANALYSIS AGILENT 7697A HEADSPACE SAMPLER A high performance system powered by intuitive software. Now there is a genuinely
Frits Hydrophobic Polyethylene Hydrophobic Polyethylene 1/8 Thick Hydrophilic Polyethylene PTFE
Empty Polypropylene Cartridges Standard Cartridge with one Hydrophobic Frit Standard Cartridge with two Hydrophobic Frits Flangeless Cartridge with one Hydrophobic Frit Frits Hydrophobic Polyethylene Hydrophobic
MO BIO Laboratories, Inc Loker Ave. West, Carlsbad, CA 92010
Application Note Determining the Best Homogenization Protocol for Any Soil Nik von Atzigen and Suzanne Kennedy, Ph.D. MO BIO Laboratories, Inc. 2746 Loker Ave. West, Carlsbad, CA 92010 Introduction Isolating
Rev F 8/30/ O Neil Rd, Macedon NY Telephone: (315) Fax: (315)
Automated 2009.01/2011.25 and AACC 32-45.01/32-50.01 Methods This document is an addendum to the ANKOM Dietary Fiber Analyzer Operator s Manual. This document does NOT replace the Operator's Manual. Rev
Chem466 Lecture Notes. Spring, 2004
Chem466 Lecture Notes Spring, 004 Overview of the course: Many of you will use instruments for chemical analyses in lab. settings. Some of you will go into careers (medicine, pharmacology, forensic science,
FAPAS Report Pesticide Residues in Green Tea. January February Page 1 of 32
FAPAS Report 19162 Pesticide Residues in Green Tea N O T C O N TR O LL ED W H EN PR IN TE D January February 2014 Page 1 of 32 PARTICIPANT LABORATORY NUMBER Participants can log in to FAPAS SecureWeb at
Flash chromatography. MN Flash adsorbents a unique variety of phases. Separation efficiency and reproducibility
Flash chromatography MN Flash adsorbents a unique variety of phases Flash columns and cartridges from MACHEREY-NAGEL are available with all CHROMABOND SPE / Flash packings (more than 40 phases, e.g., C
UPHO. ULTIMATE SAMPLE HOMOGENIZER cell disruption - user guide
UPHO ULTIMATE SAMPLE HOMOGENIZER cell disruption - user guide Cell disruption is an essential step in the workflow to extract and purify important biomolecules, such as nucleic acids and proteins. When
M-Beads Magnetic Silica Beads WAX
M-Beads Magnetic Silica Beads WAX MoBiTec GmbH 2012 Page 2 Contents Technical data... 3 Application... 4 General information... 4 Bead usage... 4 Additional materials needed... 4 Protocols... 5 Order Information,
LA-950 Laser Diffraction Analyzer
LA-950 Laser Diffraction Analyzer mark.bumiller@horiba.com Laser Diffraction Particle size 0.01 3000 µm Low End Sensitivity 30, 40, 50, 70 nm latex standards Low End Sensitivity Sensitivity: small particle
Discover and enjoy Fast Flash purifications offered by EasyVarioFlash cartridges!
Discover and enjoy Fast Flash purifications offered by EasyVarioFlash cartridges! EasyVarioFlash cartridges offer the latest improvements in Flash cartridges design. Based on the "Ready-to-Connect" concept,
RAPID, RELIABLE ADSORPTION PROCESSING
RAPID, RELIABLE ADSORPTION PROCESSING Process Scale Adsorbent Cartridges for Pharmaceutical Purification Features and Benefits Proven cartridge design assures simple, reliable adsorption processing Fixed
LC/MS/MS. Page Header. triple quadrupole mass spectrometer.
LC/MS/MS VARIAN, INC. 320-MS Page Header triple quadrupole mass spectrometer www.varianinc.com VARIAN, INC. 320-MS Unsurpassed commitment to innovation Varian, Inc. is an innovator and leader in mass spectrometry
Development of PhotonAssay Technology for Rapid Assay of Gold and Other Elements in Mineral Ores. Dr James Tickner Chrysos Corporation
Development of PhotonAssay Technology for Rapid Assay of Gold and Other Elements in Mineral Ores Dr James Tickner Chrysos Corporation Outline The need for an improved gold analysis technique The PhotonAssay
VWR SPECTROPHOTOMETERS
VWR SPECTROPHOTOMETERS Reliable Accurate Easy to Use Your first choice for spectrophotometry VWR SPECTROPHOTOMETERS Quality Guaranteed VWR SPECTROPHOTOMETERS Single Beam Units VWR offers a choice of 5 models
PRECELLYS EVOLUTION. The most efficient & flexible homogenizer
PRECELLYS EVOLUTION The most efficient & flexible homogenizer Super efficient: powerful 3D motion, up to 10 000rpm Super strong: high quality manufacturing Super flexible: 5 sizes of tubes from 0.3ml to
Enriching Beads Oligo (dt) Magnetic Beads for mrna Purification
Enriching Beads Oligo (dt) Magnetic Beads for mrna Purification Isolate the mrna transcriptome in 15 minutes User Guidance Enriching Biotechnology Rev. 1.0 October 25th. 2018 Why choose Enriching Beads
Minimizing purification time with high resolution flash chromatography
Minimizing purification time with high resolution flash chromatography David Trail*, Veronica Thomason, and John Urh Teledyne Isco Inc., P.O. Box 82531, Lincoln NE 68501 vthomason@teledyne.com (800) 228-4373
Application note: A10-011A The Effect of Filter Selection on the LOD in the Fluorometric Determination of Histamine in Seafood
Fluorimeter 627/ Application note: A1-11A The Effect of Filter Selection on the in the Fluorometric Determination of Histamine in Seafood Introduction Histamine is a biogenic amine that can be formed in
fluidic factory preview» preview» preview modular 3D printer for fluidically sealed devices Microfluidic devices in minutes for as little as $1 each
fluidic factory modular 3D printer for fluidically sealed devices preview» preview» preview Microfluidic devices in minutes for as little as $1 each fluidic factory» overview Fluidic Factory is the world
EPIGENTEK. EpiQuik Circulating Cell-Free DNA Isolation Kit. Base Catalog # P-1064 PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE
EpiQuik Circulating Cell-Free DNA Isolation Kit Base Catalog # PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE Uses: The EpiQuik Circulating Cell-Free DNA Isolation Kit utilizes magnetic beads based sizefractionation
OMNI BEAD RUPTOR Bead Mill Homogenizer
Toughest Samples. Fastest Results. OMNI BEAD RUPTOR Bead Mill Homogenizer Convenient Front-Loading Design Consistent & High-Quality Results No Cool-Down Between Runs Broadest Performance Range: 0.8m/s
30 Plex Human Luminex (Invitrogen Kit, Single Plate)
30 Plex Human Luminex (Invitrogen Kit, Single Plate) 1. Defrost samples and bring to room temperature. 2. Bring Kit components to room temperature: Wash solution 20x. Assay Diluent. Incubation buffer.
Select-a-Size DNA Clean & Concentrator MagBead Kit Catalog No. D4084 & D4085
INSTRUCTION MANUAL Select-a-Size DNA Clean & Concentrator MagBead Kit Catalog No. D4084 & D4085 Highlights Tunable: Size selection can be tuned from 100 bp to 1000 bp with left, right, or double size selection
System Parameter and Performance Comparison Between Agilent 7697A and Agilent G1888A Headspace Samplers for USP <467>
System Parameter and Performance Comparison Between Agilent 7697A and Agilent G888A Headspace Samplers for USP Technical Overview Introduction The Agilent 7697A Headspace Sampler introduced onboard
CHEM Course Outline (Part 14) Absorption Spectroscopy update 2011 For an html version of 2005 notes, click here
CHEM 524 -- Course Outline (Part 14) Absorption Spectroscopy update 2011 For an html version of 2005 notes, click here IX. Absorption Spectroscopy UV-VIS-near IR (Read Chap. 13) the most widely used analytical
NZQA registered unit standard 764 version 7 Page 1 of 6. Explain a separation and standardisation process in a dairy processing operation
Page 1 of 6 Title Explain a separation and standardisation process in a dairy processing operation Level 5 Credits 20 Purpose This theory-based unit standard is for experienced people carrying out milk
Project Planning Considerations For Emerging Contaminants. Ed Corl NAVSEA LQAO
Project Planning Considerations For Emerging Contaminants Ed Corl NAVSEA LQAO Report Documentation Page Form Approved OMB No. 0704-0188 Public reporting burden for the collection of information is estimated
APPLICATIONS. NEW GENERATION Tissue homogenizer SAMPLE PREPARATION & CELL LYSIS.
APPLICATIONS DISTRIBUTOR R&D Biotechnology Molecular biology and proteomic Genotype determination Pharmacology and Toxicology Toxic analysis, Metabolism studies Forensic Medicine DNA/RNA tests, Drugs and
Hong Kong Association for Testing, Inspection and Certification Ltd.
Hong Kong Association for Testing, Inspection and Certification Ltd. Professional Certification Scheme for Testing Personnel (Chemical Testing) 1 Examination Disclaimer Criteria, measurement techniques,
OPTIMIZE YOUR SAMPLE PREP PROTOCOL Precellys Webinar
OPTIMIZE YOUR SAMPLE PREP PROTOCOL Precellys Webinar YOUR SPEAKERS TODAY ARE 2 Stéphane Karoubi Product Manager Sophie Dubacq Application Specialist AGENDA 3 1. Sample Preparation 1. Traditional methods
Additional reagents and materials that are not supplied
sparq PureMag Beads Cat. No. 95196-005 Size: 5 ml Store at 2 C to 8 C 95196-060 60 ml 95196-450 450 ml Description sparq PureMag Beads uses reversible nucleic acid-binding properties of magnetic beads
Finer Points of ICP-OES Setup and Operation
Finer Points of ICP-OES Setup and Operation (Part 1) James Bartos Office of Indiana State Chemist Challenges with Fertilizers Broad conc ranges from low ppm to upper % level Want to test all nutrient elements
OSP Interactive Educational Programming
OSP Interactive Educational Programming Lesson Title: Pioneer Life in the Okefenokee Swamp (History & Science Connections) OSP Educational Programming: Enhance our focus on Native American and early Okefenokee
L A B O R AT O R Y A D S O R P T I O N C A R T R I D G E O P E R AT I O N M A N U A L
L A B O R AT O R Y A D S O R P T I O N C A R T R I D G E O P E R AT I O N M A N U A L E-PAK lab systems are intended for use in a controlled environment and should be set up and operated in accordance
Pharmaceutical Manufacturing and Engineering Catalog Excerpt
Pharmaceutical Manufacturing and Engineering Catalog Excerpt PME 530 Introduction to Pharmaceutical Manufacturing Pharmaceutical manufacturing is vital to the success of the technical operations of a pharmaceutical
Ultrasensitive LC MS/MS: Agilent 6470 and 6495 LC-QQQ
Ultrasensitive LC MS/MS: Agilent 6470 and 6495 LC-QQQ ROCK SOLID Performance for Trace-Level Quantitation Agilent Technologies 1 Brief History of Agilent 6400 QQQ LC/MS 6410 2006 6460 Agilent Jet Stream
Direct Polysome IP from Brain Tissue Myriam Heiman:
Direct Polysome IP from Brain Tissue Myriam Heiman: bonillm@rockefeller.edu Protocol below is for 1 IP, scale accordingly General Notes: -7 mouse striata pooled per IP -IP with 50 µg 19C8 and 50 µg 19F7
CERTIFICATE OF ANALYSIS
CERTIFICATE OF ANALYSIS ATTENTION 1000 Lewes Blvd. Whitehorse, YT Y1A 6N2 Miles Hume PO NUMBER RECEIVED / TEMP 2018-08-23 12:50 / 16 C INFO Contract No. C00044694 (Vanier) COC NUMBER B65313/14/37/38/40
ISO INTERNATIONAL STANDARD. Oilseed meals Determination of oil content Part 1: Extraction method with hexane (or light petroleum)
Provläsningsexemplar / Preview INTERNATIONAL STANDARD ISO 734-1 Second edition 2006-11-01 Oilseed meals Determination of oil content Part 1: Extraction method with hexane (or light petroleum) Tourteaux
Roger Bardsley, Applications Chemist; Teledyne Tekmar Page 1
Application Note Meeting the Korean Method ES 04607.1 for Naphthalene ( 나프탈렌 ) with Static and Dynamic Headspace GC/MS Roger Bardsley, Applications Chemist; Teledyne Tekmar Page 1 Introduction The Korean
New fraction collection features with the Agilent 220 micro plate sampler and micro plate sampling software rev. A.03. Technical Note.
New fraction collection features with the Agilent 220 micro plate sampler and micro plate sampling software rev. A.03 Technical Note Introduction The Agilent 220 micro plate sampler (MPS) is an essential
Agilent InfinityLab LC Series. Specification Compendium. Agilent Technologies
Agilent InfinityLab LC Series Specification Compendium Agilent Technologies Notices Agilent Technologies, Inc. 2014-2015, 2016 No part of this manual may be reproduced in any form or by any means (including
Print microfluidic devices in minutes for as little as $1 each.
Print microfluidic devices in minutes for as little as $1 each www.dolomite-microfluidics.com fluidic factory» overview Fluidic Factory is the world s first commercially available 3D printer for quick
Supporting Information
Momiyama, Kanan, Liu page S1 Synthesis of Acyclic!,"-Unsaturated Ketones via Pd(II)-Catalyzed Intermolecular Reaction of Alkynamides and Alkenes Norie Momiyama, Matthew W. Kanan and David R. Liu* Department
Supporting Information
Supporting Information Development of an Inexpensive RGB Color Sensor for the Detection of Hydrogen Cyanide Gas Lee A. Greenawald* a, Gerry R. Boss b, Jay L. Snyder c, Aaron Reeder d, Suzanne Bell e AUTHOR
Assay of sodium thiosulfate and ionic impurities in sodium thiosulfate using ion chromatography
APPLICATION NOTE 72502 Assay of sodium thiosulfate and ionic impurities in sodium thiosulfate using ion chromatography Authors Hua Yang and Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA, USA Keywords
lmmunoassay Antibody Purification In vitro Diagnostics, etc.
20151110 Ver. 1.0 (EN) AccuNanoBead TM Next Generation Magnetic Bead Applications Nucleic Acid Purification Cell Purification Peptide Preparation lmmunoassay Antibody Purification In vitro Diagnostics,
CATALOG & PRICE. Magnetic Beads Products
CATALOG & PRICE Magnetic Beads Products 2017 Page 1 of 9 Table of Contents PROTEIN PURIFICATION MAGNETIC BEADS...4 STREPTAVIDIN MAGNETIC BEADS...4 PROTEIN A MAGNETIC BEADS...4 GLUTATHIONE (GST-TAG AFFINITY)
LC-MS/MS Method for the Determination and Quantitation of Penicillin G and its metabolites in Citrus Fruits Affected by Huanglongbing
LC-MS/MS Method for the Determination and Quantitation of Penicillin G and its metabolites in Citrus Fruits Affected by Huanglongbing Homogenized Citrus Citrus Affected by Huanglongbing Fadi Aldeek, Ph.D.
PRINTED CIRCUIT BOARD (PCB) MICRO-SECTIONING FOR QUALITY CONTROL
SUMNotes PUBLISHED BY BUEHLER, A DIVISION OF ILLINOIS TOOL WORKS VOLUME 5, ISSUE 1 PRINTED CIRCUIT BOARD (PCB) MICRO-SECTIONING FOR QUALITY CONTROL Introduction Quality control in Printed Circuit Board
WELCOME TO THE FUTURE
WELCOME TO THE FUTURE Fully Automated LCD Recycling Robotic Leading Technology High Volume Processing Legislation Compatible and Complainant The Process is Patented Protected Economic and Environmentally
MD PRESS Advanced Wastewater Solutions
Industry s Most Advanced Technology. Experience, Not Experiments. TRIDENT TM MD PRESS Advanced Wastewater Solutions Sludge Dewatering Technology Experts REVOLUTIONARY SLUDGE DEWATERING 1.604.330.2500 1.800.799.3740
Stability of Food Emulsions (2)
Stability of Food Emulsions (2) David Julian McClements Biopolymers and Colloids Laboratory Department of Food Science Droplet Coalescence Oiling Off Coalescence Aggregation due to fusing together of two
GALILEO TMA CK 4500 HTS Tissue Microarray Platform
GALILEO TMA CK 4500 HTS Tissue Microarray Platform Tissue Microarray (TMA) A Block Of Samples From Hundreds Of Blocks (S. M. Hewitt, M.D., Ph.D., Tissue Array Research Program, LP, CCR, NCI, NIH) TMA technology
New Certified Reference Materials for Chromatography
New Certified Reference Materials for Chromatography Dr. Frank Michel Frank.michel@sial.com sigma-aldrich.com/analytical 2012 Sigma-Aldrich Co. All rights reserved. Use of Certified Reference Materials
Introduction to Microfluidics. C. Fütterer, Institut Curie & Fluigent SA, Paris
Introduction to Microfluidics C. Fütterer, Institut Curie & Fluigent SA, Paris Miniaturisation & Integration Micro-Pipettes Problems: Minimal volume: 1μl Samples unprotected against evaporation & contamination
Nexera Method Scouting
Ultra High Performance Liquid Chromatograph Method Scouting System Nexera Method Scouting C190-E150A Maximizing Efficiency for Method Development The Nexera Method Scouting System design is based on the
Determination of the N-Nitrosamine Content in Rubber Articles Using the Agilent 7000A Triple Quadrupole GC/MS System
Determination of the N-Nitrosamine Content in Rubber Articles Using the Agilent 7A Triple Quadrupole GC/MS System Application Note Consumer Products Authors Yun Zou and Chongtian Yu Agilent Technologies
FIRECLAY SINKS PRODUCT KNOWLEDGE. Franke,
FIRECLAY SINKS PRODUCT KNOWLEDGE Franke Fireclay Sink Manufacturers Many Franke fireclay sinks are manufactured in France and Germany by Villeroy & Boch Apron-front models MHK110-20 MHK110-24 MHK710-20
Downloaded from:
Corran, PH; Cook, J; Lynch, C; Leendertse, H; Manjurano, A; Griffin, J; Cox, J; Abeku, T; Bousema, T; Ghani, AC; Drakeley, C; Riley, E (2008) Dried blood spots as a source of anti-malarial antibodies for
Microplate Reader EPC / PRODUCTS / APPLICATION / SOFTWARE / ACCESSORIES / CONSUMABLES / SERVICES. Analytical Technologies Limited
Turnkey Laboratories Solutions Microplate Reader M- 2201 EPC / PRODUCTS / APPLICATION / SOFTWARE / ACCESSORIES / CONSUMABLES / SERVICES Analytical Technologies Limited An ISO 9001 Certified Company www.analyticalgroup.net
Level Control Methods and Explanations for Common Electrical Control terms. John A. Evans President, Motor Protection Electronics
Level Control Methods and Explanations for Common Electrical Control terms John A. Evans President, Motor Protection Electronics A Quick Florida Joke! Control Topic # 1 Level Sensing Options Level Sensing
OptiFlex 2. any powder any part any place. Your global partner for high quality powder coating. Manual powder coating
OptiFlex 2 any powder any part any place Manual powder coating The new OptiFlex 2 puts the world s most advanced manual powder coating technology in the palm of your hand. Spray all powders with ease,
Tel.: ; Fax: Spain. Tel.: ; Fax: *Corresponding author:
POLYAMIDE AND POLYPROPYLENE TEXTILE DYEING USING SUPERCRITICAL CARBON DIOXIDE (sc-co2) Garay, I. 1* ; Pocheville. A. 1 ; Hernando, I. 1 ; Fages, E. 2 ; Gironés, S. 2 and Ferrándiz, M 2. 1 GAIKER-IK4. Parque
BIOL110L-Cell Biology Lab Spring Quarter 2012 Module 3-4 Wednesday May 30, 2012
BIOL110L-Cell Biology Lab Spring Quarter 2012 Module 3-4 Wednesday May 30, 2012 PART I: Isolation of over-expressed GFP-Karyopherins from yeast extracts by affinity capture on nucleoporins Summary: The
LOABeads Protein A. Product no Product Manual. Lab on a Bead AB
Product no 1001 LOABeads Protein A Product Manual Lab on a Bead AB Revision date 2016-03-08 Copyright 2015-2016 Lab on a Bead AB All rights reserved Table of Contents 1. General information 3 2. Antibody
TrueBlot Protein G Magnetic Beads PG ml. TrueBlot Protein G Magnetic Beads PG ml. Bead Mean Diameter 0.5 µm. Bead Concentration
Rockland s TrueBlot Protein G Magnetic Beads are uniform, non-aggregating, super-paramagnetic beads coupled with a biomolecule, such as Protein G. These beads are specifically designed, tested and quality
mag maxi kit Intended use of the mag maxi kits
mag maxi kit For in vitro diagnostic use 40403 40430 10 288 May 2014 LGC Genomics GmbH Ostendstr. 25 TGS Haus 8 12459 Berlin Germany Tel: +49 (0)30 5304 2200 Fax: +49 (0)30 5304 2201 Intended use of the
T A I N S T R U M E N T S
TA INSTRUMENTS TA HISTORY OF INNOVATION 1962-900 THERMAL ANALYZER FIRST COMMERCIAL DSC 1968 - PRESSURE DSC 1976 - FIRST COMMERCIAL DMA TA Instruments history of meeting customer needs for high technology
FlowPhase Examples Page 1 of 15. Table of Contents
FlowPhase Examples Page 1 of 15 Table of Contents 1. VLE Flash... 2 1.1 Basic Input... 2 1.2 Entering Components... 3 1.3 Entering mol fractions... 3 1.4 Performing a flash... 4 1.5 Changing Units... 4
Aquaphotomics & NIR hyperspectral imaging
Aquaphotomics & NIR hyperspectral imaging tools for understanding the role of water in foods aoife.gowen@ucd.ie School of Biosystems Engineering, University College Dublin, Ireland. Key Questions What
Procedure & Checklist - Greater Than 10 kb Template Preparation Using AMPure PB Beads
Procedure & Checklist - Greater Than 10 kb Template Preparation Using AMPure PB Beads Before You Begin This procedure can be used to prepare greater than 10 kb libraries from 5 μg of sheared and concentrated
SPECIFICATION CHIP LED DEVICE. [Contents]
![SPECIFICATION CHIP LED DEVICE. [Contents]](/thumbs/80/81813068.jpg "SPECIFICATION CHIP LED DEVICE. [Contents]")
*Customer: SPECIFICATION ITEM MODEL Revision Date CHIP LED DEVICE [Contents] 1. Features 2. Absolute maximum ratings 3. Electro-optical characteristics 4. Characteristic diagram 5. Soldering profile 6.
3. Click on the tab that say Footprint Basics. Read the information and answer the questions below:
Name: _ Block: Date: ECOLOGICAL FOOTPRINT Each of us consumes some of the Earth s products and services every day. How much we take depends on the ways in which we satisfy our needs and wants the many
.water.gas.hygienic.micro
.water.gas.hygienic.micro .water.gas.hygienic.micro Micro is a customer-oriented concept to combine the physical attributes of the application environments from your world. In this overview you will find
General information 1 / 17
General information 1 / 17 SF 6 -gas Sulphur hexafluoride (molecule) Sulphur hexafluoride is a non-toxic, inert, insulating and quenching gas of high dielectric strength and thermal stability. colourless
Supporting Information
Supporting Information rganocatalytic Mitsunobu Reactions Tracy Yuen Sze But and Patrick H. Toy * Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, People s Republic of China
Agilent 1200 Infinity Series
Agilent 1200 Infinity Series Specification Compendium Agilent Technologies Notices Agilent Technologies, Inc. 2010-2012, 2013 No part of this manual may be reproduced in any form or by any means (including
2,4 and 2,5-bis(benzooxazol-2 -yl)hydroquinone (DHBO) and their borate complexes: Synthesis and Optical properties
Electronic Supplementary Material (ESI) for ew Journal of Chemistry. This journal is The Royal Society of Chemistry and the Centre ational de la Recherche Scientifique 2016 Supplementary Material 2,4 and
Syncore Analyst Technical data sheet
STOP PROGRAMM SET DOWN UP DOWN UP Syncore Analyst Technical data sheet With the Syncore Analyst, BUCHI is offering a solution for a fast, gentle and environmentfriendly multiple sample concentration to
KASD Disposable Syringe Filter
KASD Disposable Syringe Filter KASD Syringe Filters are syringe-operated filters for the clarification of aqueous solutions (column eluates, tissue culture additives, HPLC samples, etc.). It is further
LVEM 25. Low Voltage Electron Microscope Fast Compact Powerful.... your way to electron microscopy
LVEM 25 Low Voltage Electron Microscope Fast Compact Powerful... your way to electron microscopy INTRODUCING THE LVEM 25 High Contrast & High Resolution Unmatched contrast of biologic and light material
The transducer component converts the measured signal and displays the actual value.
Digital inductive conductivity transmitter System Integration Provides low Total Cost of Ownership PEEK/PPA version for CIP applications TEACH IN and SIMULATION Function Approval PVDF Version PEEK Version
Nicolet Almega XR. High Performance Micro and Macro Dispersive Raman Analysis Systems
m o l e c u l a r s p e c t r o s c o p y Nicolet Almega XR High Performance Micro and Macro Dispersive Raman Analysis Systems Materials Research Identification/Verification Characterization Failure Analysis
NGC Chromatography Systems
Chromatography NGC Chromatography Systems Comprehensive Solutions for Protein Purification DESIGNED BY YOU. BUILT BY BIO-RAD. NGC Medium-Pressure Chromatography Systems The NGC instrument is an automated
Thermal Conductivity Sensor for Leak or Pressure Detection MTCS2601. MTCS2601 silicon sensing die in SMD ceramic package
Sensor Description Thermal Conductivity Sensor for Leak or Pressure Detection MTCS2601 MTCS2601 silicon sensing die in SMD ceramic package Thermal conductivity sensor for primary vacuum measurement Silicon