lmmunoassay Antibody Purification In vitro Diagnostics, etc.

Transcription

1 Ver. 1.0 (EN) AccuNanoBead TM Next Generation Magnetic Bead Applications Nucleic Acid Purification Cell Purification Peptide Preparation lmmunoassay Antibody Purification In vitro Diagnostics, etc. 8-11, Munpyeongseoro, Daedeok-gu, Daejeon, 34302, Republic of Korea Tel: (Korea) (International) sales@bioneer.com

2 Product Description Recently, many of bio related institutes and industries are approaching their research applying magnetic process. The principle of magnetic beads are coating with appropriate functional group in the surface of magnetic beads. With the functional group, magnetic beads can combine protein and then use external Features of AccuNanoBead TM Fast Binding guaranties higher throughput automation Large Surface Area enables better sensitive assay Globular structure increases Uniform Spheres and Much Smaller than Others Figure1. FE-SEM and TEM Image of Magnetic Beads SEM photographs of magnetic Beads from: (a) Bioneer (AccuNanoBead ) (b) company A (c) company B (d) company C ; (e) company D.

3 Eliminate Repeated Pipetting or Centrifugation Simpler Centrifugation Repeated Pipetting agarose beads needed centrifugation and repeatated pipetting. For this reason, each step. AccuNanoBead enables user to remove supernatant easily without lose of samples, because it can be immobilized by magnet. Complete Recovery within 1 Second Faster Figure 3. Recovery test of Magnetic Beads from AccuNanoBead (Dark Blue) and Competitor L (Brown) Recovery of AccuNanoBead is completed within 1 second. It is 15 times faster than Competitor L product. Rapid recovery allows More AccuNanoBead is notably higher than competitors products. This is due to increased surface area

4 Products of AccuNanoBead TM AccuNanoBead Silica Magnetic Nano Beads AccuNanoBead Protein G Magnetic Nano Beads Matrix AccuNanoBead Epoxy Magnetic Nano Beads AccuNanoBead C18 Silica-coated Fe3O4 400 nm Average size Ligand - OH Protein G Epoxy C18 Working Temp C 4 C- room temp. 4 C- room temp C Storage Store at room temperature upon receipt Store at 4 C upon receipt Store at 4 C upon receipt Store at room temperature upon receipt AccuNanoBead TM C18 Magnetic Nano Beads AccuNanoBead TM C18 Nano Beads is silica magnetic nano beads containing hydrophobic C18 alkyl groups on the surface. It binds to peptides or proteins using strong hydrophobic absorption interaction. Therefore, beads can be spectrometry (MS) analysis. AccuNanoBead TM Epoxy Magnetic Nano Beads AccuNanoBead TM Epoxy Magnetic Nano Beads is silica magnetic nano beads coated with epoxy ligands on the surface. Beads can form covalent bond with hydroxyl-, amine and thiol- ligands. Coupling reaction of each ligand is favored at ph 11-12, ph>9 and ph , respectively. Epoxy ligand is able to react with these ligands. It can be applied to cell sorting, immunoprecipitation, and etc. - R- CH2- CH - CH2 O Figure 5. Epoxy-Coupling Reaction + R -OH R -SH R -NH2 O - R - CH2 - CH - CH - X - R 2

5 AccuNanoBead TM Protein G Magnetic Nano Beads AccuNanoBead TM Protein G Magnetic Nano Beads is product coated with Protein G on the surface of silica magnetic Loading Binding Elution Washing AccuNanoBeadTM Protein G Magnetic Nano Beads Antibody Target Non-Target Protein M 97kDa 66kDa 45kDa W Whole blood M U W Pl a Plasma L U sm a W ho le bl oo d AccuNanoBead Protein G Magnetic Nano Beads Magnetic beads mixed with protein M N IgG E F Heavy chain 29kDa Light chain 20.1kDa 14.4kD 6.5kDa Sample Sample amount used Antibody Human Plasma 100 μl 91 μg Human whole blood 100 μl 109 μg ExiProgen ExiProgen ExiProgen. M : protein marker, L: loading fraction, U: unbound fraction, W: washing fraction, N:negative control (only beads), IgG: Human IgG, E: elution fraction. AccuNanoBead with a competitor (a) Eluted fraction after binding of Human IgG followed by washing through Protein G magnetic bead. Upper and respectively. (b) Comparison of binding capacity with competitor s product.

6 Ordering Information Cat no. Size Product TA Silica Magnetic Nanobeads, size 400 nm /25 ml TA Silica Magnetic Nanobeads, size 400 nm 1.0 g/50 ml TA Silica Magnetic Nanobeads, size 400 nm 10 g/500 ml TA NH2 Magnetic Beads, size 400 nm TA COOH Magnetic Beads, size 400 nm TA Epoxy Magnetic Beads, size 400 nm TA C18 Magnetic Beads, size 400 nm TA Biotin Magnetic Beads, size 400 nm /25 ml TA Ni-NTA Magnetic Beads, size 400 nm /25 ml TA Thiol Magnetic Beads, size 400 nm TA Protein G Magnetic Nanobeads, size 400 nm 40 mg/1 ml TA Protein G Magnetic Nanobeads, size 400 nm 200 mg/10 ml TA Protein G Magnetic Nanobeads, size 400 nm 500 mg/25 ml Contact Us Bioneer Corporation Bioneer Inc. Bioneer R&D Center 8-11 Munpyeongseo-ro, Daedeok-gu Daejeon, 34302, Republic of Korea Tel: (Korea: ) sales@bioneer.com 155 Filbert St. Suite 216 Oakland, CA 94607, USA Toll Free: Fax: order.usa@bioneer.us.com Korea Bio Park BLDG #B Daewangpangyo-ro, Bundang-gu, Seongnam-si Gyeonggi-do, 13488, Republic of Korea Tel: Fax: