MagSi Beads. Magnetic Silica beads. and In-Vitro Diagnostics

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1 MagSi Beads Magnetic Silica beads for Research in Life Science and InVitro Diagnostics

2 Wide range of products for numerous Applications AMS Biotechnology supplies a unique range of magnetic and nonmagnetic silica beads in the range from 150 nm up to 5 µm for customized beads and own bead production. Our standard products have sizes of 600 nm and 1 µm and 1.2 µm. For most applications the 1 µm beads are ideal. The 600 nm particles give more surface area per mg of bead but the magnetic strength slightly decreased. The 1.2 µm beads are used for genomics and proteomics applications where highest magnetic strength is needed. Magnetic strength Narrow size distribution Surface area 600 nm µm The fast and flexible production process facilitates unrestricted customization possibilities with regard to sizes, kinetics, optical, surface and binding properties. MagSi beads are specifically designed for separation, concentration and purification of biomolecules and specific cells or cell compartments. They suit ideally as a solid phase in assay kits. 1. MagSi s Coating our magnetic silica beads with Streptavidin, Protein A, protein G or other ligand specific molecules allows isolating of specific target molecules or cells out of a large volume or complex matrix. In our beads we can also encapsulate fluorescent dyes or quantum dots which allows imaging and quantitative detection in automated systems. The MagSiTools are ideal for your own experiments and coupling procedures. MagSi magnetic beads are designed to meet the specific needs in typical biological applications. We know that scientists have a need for state of the art, innovative products addressing forefront applications. MagSi Advantages High customization potential (e.g. sizes, surfaces, colors) Speed & Robustness Flexibility & Scalability Ideal for automated systems High binding capacity nm & µm sized magnetic beads proved for downstream applications like PCR etc. Minimal nonspecific absorption

3 Bead Application Surface Encapsulation possibilities MagSiTools Customer development Tailored products Silica COOH, NH2 SH CHO MagSiSTA Isolate biotinylated target molecules Immuno assays Bind biotinylated antibodies Cell/bacteria isolation Protein purification Streptavidin covalently bound to silica MagSiprotein A MagSiprotein G Bind IgG antibodies Antibody purification Immuno precipitation from small sample volumes Protein A or G covalently bound to silica MagSiProteomics Protein Purification Concentration and/or desalting of peptide and protein digests C4, C8 or C18 MagSi beads for DNA isolation isolation of genomic DNA PCR clean up DNA sequencing plain silica COOH MagSiWCX Sample preparation Prefractionation prior to mass spectrometry SDSPAGE analysis Protein and peptide separation Biomarker analysis Serum/plasma profiling MagSi with Target Specific Ligand Cell capturing Protein isolation Immuno assays Target specific ligand directly bound on bead surface, e.g.: CD4, CD14 antibodies

4 1.1 MagSiTools The MagSiS platform has a broad range of functionalisation possibilities such as COOH, NH 2, SH or CHO. It is a versatile and adaptable solid phase in biomagnetic separations and component of diagnostic kits therefore ideal for your own specific modification and development. MD16003 MagSiS 600 MD01005 MagSiSNH2 1.0 MD18003 MagSiS 600 MD03005 MagSiSNH2 1.0 MD19003 MagSiS 600 MD04005 MagSiSNH2 1.0 MD01003 MagSiS 1.0 MD16006 MagSiSSH 600 MD03003 MagSiS 1.0 MD18006 MagSiSSH 600 MD04003 MagSiS 1.0 MD19006 MagSiSSH 600 MD16004 MagSiSCOOH 600 MD01006 MagSiSSH 1.0 MD18004 MagSiSCOOH 600 MD03006 MagSiSSH 1.0 MD19004 MagSiSCOOH 600 MD04006 MagSiSSH 1.0 MD01004 MagSiSCOOH 1.0 MD16007 MagSiSCHO 600 MD03004 MagSiSCOOH 1.0 MD18007 MagSiSCHO 600 MD04004 MagSiSCOOH 1.0 MD19007 MagSiSCHO 600 MD16005 MagSiSNH2 600 MD01007 MagSiSCHO 1.0 MD18005 MagSiSNH2 600 MD03007 MagSiSCHO 1.0 MD19005 MagSiSNH2 600 MD04007 MagSiSCHO 1.0 s > 100 ml on request 1.2 MagSiProteomics The MagSiproteomics C18 beads are an ideal tool for the purification, concentration and desalting of peptides and protein digests. MagSiproteomics C8 beads represent an intermediate hydrophobicity (less hydrophobic than C18 and more hydrophobic than C4 beads) and are suitable for sample preparation in context to proteomic profiling and biomarker research. The relatively low hydrophobicity of the C4 beads allows the purification and fractionation of larger biomolecules like proteins. MD01014 MagSiproteomics C4 MD03015 MagSiproteomics C8 MD02014 MagSiproteomicsC4 MD01009 MagSiproteomics C18 MD03014 MagSiproteomics C4 MD03009 MagSiproteomics C18 MD01015 MagSiproteomics C8 MD04009 MagSiproteomics C18 MD02015 MagSiproteomics C8 s > 100 ml on request

5 1.3 MagSiSTA MagSiSTA beads are uniform silica particles with a monolayer of high quality streptavidin covalently attached to the bead surface. They come as readytousesystem. They can be used to isolate biotinylated proteins or other biotinylated molecules. The magnetic properties allow easy an quick washing steps in ELISA assays and protein or DNA/RNA isolations. In addition, since beads are in suspension and therefor the coupled antibody the incubation time can be shortened when compared to ELISA tests using antibody coated micro plates. 1.4 MagSiprotein A and G Protein A and protein G are recombinant proteins that bind with high affinity to the Fc portion of several classes and subclasses of immunoglobulin from a variety of species. Both proteins form reversible complexes with different IG subclasses and species. MagSiprotein A/G is is an ideal tool for efficient and economical purifications and immunoprecipitations from small sample volumes. The recombinant protein G (~26kDa) on the surface of our beads contain only IgG binding sites. The albumin, cell wall and cell membrane binding domains, present on the wildtype protein G, have been removed to optimize binding properties. The 42kDa protein A is from Staphylococcus aureus. The advantage of protein G an A beads, compared to Streptavidin beads, is that the antibody does not need to be biotinylated and the binding is reversible. So they are ideal for protein isolation and proteomics. Also, the magnetic properties allow easy and quick washing steps in protein isolations. MD16001 MagSiSTA 600 MD10012 MagSiprotein G 600 MD18001 MagSiSTA 600 MD11012 MagSiprotein G 600 MD19001 MagSiSTA 600 MD01012 MagSiprotein G 1.0 MD01001 MagSiSTA 1.0 MD02012 MagSiprotein G 1.0 MD03001 MagSiSTA 1.0 MD10013 MagSiprotein A/G 600 MD04001 MagSiSTA 1.0 MD11013 MagSiprotein A/G 600 MD10011 MagSiprotein A 600 MD01013 MagSiprotein A/G 1.0 MD11011 MagSiprotein A 600 MD02013 MagSiprotein A/G 1.0 MD01011 MagSiprotein A 1.0 MD02011 MagSiprotein A 1.0 s > 100 ml (and > 5 ml for MagSiprotein A or G) on request 1.5 MagSiGiant Quantum Dots AMSBIO can supply magnetic MagSi beads loaded with single quantum dots (QD). This allows combination of imaging, detection and quantification using the above features (Streptavidin, protein A/G). The packaging of QD further enhances current detection limits. MagSiGiant Quantum Dots are offered for client specific applications as MagCustom project.

6 1.6 MagSi beads and kits for DNA isolation and purification MagnaMedcsi has developed several distinct magnetic bead types for nucleic acid isolation applications: High throughput applications supported by robotic liquid handling stations typically need magnetic beads with strong magnetic response whereas highly complex, manual DNA extraction applications needs magnetic beads with highest DNA capture, DNA selectivity and lowest unspecific binding. For details please view our selection guide for genomics applications! 1.7 silica MagSi magnetic beads for nucleic acid isolation MagSi magnetic silica beads with high magnetic content optimized for nucleic acid isolation from various sources (blood, cells, bacteria etc.) for robotic and manual workflow. MD01017 MagSiDNA 300 mg/ml 300 nm MD02017 MagSiDNA 300 mg/ml 300 nm 10 ml MD03017 MagSiDNA 300 mg/ml 300 nm 100 ml MD01018 MagSiDNA allround 20 mg/ml MD02018 MagSiDNA allround 20 mg/ml 10 ml MD03018 MagSiDNA allround 20 mg/ml 100 ml 1.8 carboxylated MagSi beads for nucleic acid isolation The carboxylated surface guarantees best results in terms of DNA purity and yield. These beads are specially recommended for high DNA yield applications like isolation of viral DNA, DNA sequencing or PCR clean up. MD01019 MagSiDNA COOH 300mg/ml 300nm MD02019 MagSiDNA COOH 300mg/ml 300nm 10 ml MD03019 MagSiDNA COOH 300 mg/ml 300nm 100 ml MD01020 MagSiDNA allround COOH MD02020 MagSiDNA allround COOH 20 mg/ml 1.2 µm 10 ml MD03020 MagSiDNA allround COOH 1.2 µm 100 ml 1.9 MagSigDNA Blood Kit MagSigDNA Blood Kit for isolation of gdna from human and mammalian whole blood or buffy coat, non mammalian blood samples, cerebrospinal fluids and bone marrow, gdna from swabs or rinsed liquid from swabs. Volume MD60001 MagSigDNA Blood Kit 1,000 isolations MD60002 MagSigDNA Blood Kit 10,000 isolations 1.10 MagSi DNA Clean Kit MagSiDNA Clean Kit for purification and concentration of DNA fragments from enzymatic reactions like PCR products, cdna synthesis, enzyme restriction digestions and removal of DyeDeoxyTM terminators from DNA cycle sequencing reactions of PCRproducts and Plasmids after use ABI Prism TM terminator Kits Volume MD60013 MagSi DNA Clean Kit 150 purifications MD60014 MagSi DNA Clean Kit 15,000 purifications

7 1.11 MagSiWCX and MagSiWAX MagSiWCX (weak cation exchange) and MagSiWAX (weak anion exchange) beads are an ideal tool for the reduction of protein or peptide complexity (e.g. cell lysates). Main applications include: Sample preparation and prefractionation prior to mass spectrometry (e.g. MALDITOF analysis) SDSPAGE analysis Protein and peptide separation for multiple downstream applications Biomarker analysis and serum/plasma profiling The high magnetic strength makes them applicable for both manual and automated/robotic fractionation, because the beads will typically collect in less than 1 minute when magnetic force is applied. This quick and complete separation gives very good reproducibility since no beads will be lost during washing steps. The quick protein adsorption, desorption and magnetic collection significantly shortens the protocol time over conventional column based ion exchange chromatography. MagSiWAX MD01025 MagSiWAX MD02025 MagSiWAX MagSiWCX MD01023 MagSiWCX MD04023 MagSiWCX 10 ml 1.12 MagCustom Tailor made magnetic and non magnetic beads are available in small, mid and large scales and at reasonable prices Art. description Phase MD80001 MagCustom Feasibility Study Phase 1 MD80002 MagCustom Project Define Phase Phase 2 MD80003 MagCustom ion Phase Phase Separation Tools Art. nr. Description MD90001 MMSeparator M Magnetic separator for isolation of MagSi beads in 12x 1,5 ml and 12x 2 ml tubes. MD90002 MMSeparator M96 Magnetic separator for isolation of MagSi beads in microtiterplate format (96 wells) MD90003 MMSeparator 25 Magnetic separator for volumes up to 25 ml MD90004 MMSeparator 250 Magnetic separator for volumes up to 250 ml

8 Applications 2.1 Biocompatibility Different tests in blood and cell cultures show that MagSi beads have little to no toxicity. When incubating lung cells (A549) with regular MagSi silica beads, no increase in IL6 or IL8 was visible up to 500 µg/ml of beads. Comparable results were found for human whole blood. This means our beads are suitable for applications in living cells or blood when you want to keep the cells viable and the level of cell stress very low. 2.2 Cytokines in whole blood 0,8 Con IL6 and IL8 (ng/ml) 2. IL6 (ng/ml) 0,6 IL8 (ng/ml) 0,4 Blank 0,2 0, Conc MagSi beads (µg/ml) IL6 and IL 8 production in blood after 4h incubation with MagSiS beads Protein assay MagSi Streptavidin beads can be used for ELISA assays in micro plates. A biotinylated primary antibody is bound to the streptavidin to isolate the target protein, followed by detection of that protein with a labeled second antibody. OD 405nm 4 3 To measure IgG, a biotinylated antiigg is bound to the MagSiSAV bead. After incubation of the beads with an analyte, a second antibody labeled with peroxidase is added. Then substrate (ABTS*) is added and the green color development measured via a micro plate reader (absorption at 405nm, corrected for dilution). There is no difference visible between the MagSi beads (dashed line) and the reference beads (solid line) IgG (ng/ml) * 2,2 Azinobis(3ethylbenzothiazoline6sulfonic acid) When testing our streptavidin beads in an automated system (e.g. Bioveris MSERIES ) the noise level is very low compared to references and they have good reproducibility. 2.3 Minimal nonspecific absorption 1 The knowhow in our company and flexibility of production has allowed us to develop beads with low background absorption. When a protein needs to be isolated from a mix of other proteins, this background absorption determines the yield and purity of your target protein. MagSi bead tailored blocking methods reduces the nonspecific binding of proteins to an absolute minimum. In the image on the right side there is a SDS PAGE gel of MagSiSTA and MagSiprotein A/G beads after incubation with bovine IgG and BSA (fraction V) solution. For the protein G beads (lane 24 and 6), the IgG bands are clearly visible. The protein A bead (lane 5) doesn't show IgG, because the affinity for bovine IgG is very low. The streptavidin beads (lane 7) show no protein binding. No background absorption of BSA is visible for all the samples SDS PAGE after incubating beads in BSA solution. Lane 1: marker, lane 2 reference sample, lane 37 MagSi beads Downstream applications Tests show that MagSi beads don't interfere with downstream applications. When performing realtime PCR, there is no inhibition visible. Also when performing protein isolation, no artifacts occur during SDSPAGE or spectrometry. The same is true for biochemical assays like an ELISA.

9 2.5 Imaging Beads with a fluorescent dye or quantum dots make them perfectly visible under a fluorescent microscope. This means, specific cells can be visualized using fluorescent beads coated with an antibody (direct, via protein A/G or via streptavidin). In some cases, the cells can then be isolated by using the magnetic properties of the beads. < 4 samples of fluorescent labeled beads, a: Singel Quantum Dots, b: Quantum Dots in a vial, c: beads with rhodamin incapsulated, d: MagSi beads coated with single quantum dots 2.6 Nucleic acid isolation 1 The porous surface of the silica beads engineered for DNA/RNA isolation gives them a high surface area and high binding capacity. This makes sure that nearly all the DNA is isolated from the sample. The beads are ideal when using them to isolate DNA from cell lysates or whole blood. The high (up to 90%) concentration of ferro fluid makes it very easy to collect the beads, using a magnet, in the rinsing steps. Within seconds, all the beads are collected to the magnet. This high magnetic strength makes them also applicable in automated systems because it reduces loss of sample in the rinsing steps Agarose electrophoresis. Lane 1 marker, 27 RNA isolation under different conditions, lane 8 DNA isolation Protein isolation Reference MagSi MagSi beads excel with superior capacity in binding proteins. Example: as a customized carrier for Strep Tag from IBA GmbH, MagSi clearly outperform beads from references. < Test results from an E.coli cell lysate. 2.8 Proteomics Reversed phase and ion exchange magnetic beads (MagSiWCX; MagSiWAX) are ideal tools for typical proteomics applications: desalting of proteins, peptides and proteolytical digests prior to mass spectrometry concentration of low concentrated proteins or peptides fractionation of a complex peptide or protein mixture Use of magnetic beads is an economical, safe and easytouse alternative to commonly used pipette or column based methods.

10 Information for Ordering You can order by phone, fax, or online. For a fast and efficient service please provide the following order information: Delivery address Invoice address Purchaser contact telephone number End user name and telephone number (if different) Purchase order number name and catalogue number Quantity and size of products Indicate if you account is VAT exempt There is no minimum order requirement. Magnet Effect #1 Get your perfect match immediatly Magnet Effect #2 Save your time, and money Magnet Effect #3 Easier that traditional methods

11 Time is ready for a change? You have a need for customized beads even in small scale?