Recombinant com1 Attachment to Polystyrene Beads: A Surface Display Mimic for C. burnetii
|
|
|
- Margaret Blankenship
- 5 years ago
- Views:
Transcription
1 Recombinant com1 Attachment to Polystyrene Beads: A Surface Display Mimic for C. burnetii Dan Read, Ph.D. Battelle Dugway perations Life Sciences Division Dugway Proving Ground readd@dpg.army.mil
2 Report Documentation Page Form Approved MB No Public reporting burden for the collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden, to Washington Headquarters Services, Directorate for Information perations and Reports, 1215 Jefferson Davis Highway, Suite 1204, Arlington VA Respondents should be aware that notwithstanding any other provision of law, no person shall be subject to a penalty for failing to comply with a collection of information if it does not display a currently valid MB control number. 1. REPRT DATE 01 CT REPRT TYPE N/A 3. DATES CVERED - 4. TITLE AND SUBTITLE Recombinant com1 Attachment to Polystyrene Beads: A Surface Display Mimic for C. burnetii 5a. CNTRACT NUMBER 5b. GRANT NUMBER 5c. PRGRAM ELEMENT NUMBER 6. AUTHR(S) 5d. PRJECT NUMBER 5e. TASK NUMBER 5f. WRK UNIT NUMBER 7. PERFRMING RGANIZATIN NAME(S) AND ADDRESS(ES) Battelle Dugway perations Life Sciences Division Dugway Proving Ground 8. PERFRMING RGANIZATIN REPRT NUMBER 9. SPNSRING/MNITRING AGENCY NAME(S) AND ADDRESS(ES) 10. SPNSR/MNITR S ACRNYM(S) 12. DISTRIBUTIN/AVAILABILITY STATEMENT Approved for public release, distribution unlimited 11. SPNSR/MNITR S REPRT NUMBER(S) 13. SUPPLEMENTARY NTES See also ADM001851, Proceedings of the 2003 Joint Service Scientific Conference on Chemical & Biological Defense Research, November 2003., The original document contains color images. 14. ABSTRACT 15. SUBJECT TERMS 16. SECURITY CLASSIFICATIN F: 17. LIMITATIN F ABSTRACT UU a. REPRT unclassified b. ABSTRACT unclassified c. THIS PAGE unclassified 18. NUMBER F PAGES 39 19a. NAME F RESPNSIBLE PERSN Standard Form 298 (Rev. 8-98) Prescribed by ANSI Std Z39-18
3 Biological Agent Simulants Need for biological simulants (point detection) Actual biological materials release is dangerous and restricted by law Replace live agent but maintain agent properties: Physical (size, aerosolization ) Detection (surface antigens) Joint Biological Point Detection System (JBPDS)
4 Current Simulants Used for Field Tests Bacillus atrophaeus (BG)--Gram-pos. spore former Erwinia herbicola (EH)--Gram-neg. vegetative Male-specific bacteriophage type 2 (MS2)--viral valbumin (V)--protein toxin Good preservation of many physical characteristics Detection properties (surface antigens) not represented Need more realistic simulants for point detection testing
5 Bead Technology Approach Identify agent-specific surface antigen Produce antigen (recombinant) Attach recombinant antigen to surface of a microsphere (polystyrene bead) Bead simulant mimics surface of agent Potentially detected by point detection systems (immunoassay) Ag Bead
6 Bead Simulant for Coxiella burnetii Coxiella uter Membrane Protein 1 (com1) Surface protein of Coxiella burnetii Most immunodominant protein Unique to Coxiella Good target antigen candidate
7 Bead Simulant Project Goals Test feasibility of attaching recombinant antigen to polystyrene beads Test two different approaches for antigen attachment Passive adsorption (hydrophobic) Covalent linkage (covalent bond) Beads bind com1 antigen? Bound com1 recognized by α-coxiella Abs? (Bead simulant mimic Coxiella surface?)
8 ELISA 1. Determine if com1 attached to beads 1. Passive adsorption 2. Covalent coupling 2. Compare antigenicity of com1 via passive or covalent attachment to beads vs. Coxiella 1 Antibody: α-com1 mono/polyclonal antibody 2 Antibody: conjugated to HRP
9 Detection of com1 Attached to Beads by Passive Adsorption and Covalent Linkage α-com1 Monoclonal Antibody com1 Detected (D650) Covalent Linkage Coxiella 200 µg/ml com1 20 µg/ml com1 2.0 µg/ml com1 0.2 µg/ml com µg/ml com1 Passive Adsorption Coxiella 200 µg/ml com1 20 µg/ml com1 2.0 µg/ml com1 0.2 µg/ml com E E E E E E µg/ml com1 Beads or rganisms Per ml
10 Detection of com1 Attached to Beads by Passive Adsorption and Covalent Linkage α-coxiella Polyclonal Antibody (PE-CBD) com1 Detected (D650) E E E E E E+09 Covalent Linkage Coxiella 200 µg/ml com1 20 µg/ml com1 2.0 µg/ml com1 0.2 µg/ml com µg/ml com1 Passive Adsorption Coxiella 200 µg/ml com1 20 µg/ml com1 2.0 µg/ml com1 0.2 µg/ml com µg/ml com1 Beads or rganisms Per ml
11 Flow Cytometry 1. Compare size distribution of passive and covalent beads vs. Coxiella Forward scatter (relative size) 2. Compare com1 binding distribution across each bead population vs. Coxiella Relative fluorescence 1 Antibody: α-com1 monoclonal antibody 2 Antibody: conjugated to FITC
12 Flow Cytometry of Polystyrene Beads Relative Size Distribution (FS) Coxiella Passive Adsorption Beads Covalent Coupling Beads Number Number Number FS LG FS LG FS LG
13 Flow Cytometry of Polystyrene Beads Relative Fluorescence (FL) Coxiella Passive Adsorption Beads Covalent Coupling Beads
14 Immuno Scanning Electron Microscopy Compare uniformity of com1 attachment on individual beads (passive vs. covalent) 1 Antibody: α-com1 monoclonal antibody 2 Antibody: conjugated to colloidal gold (25 nm)
15 Covalent Coupling Beads No com1 7.4 µm
16 Covalent Coupling Beads w/ com1 25 nm gold particles
17 Passive Adsorption Beads No com1 9.9 µm
18 Passive Adsorption Beads w/ com1 25 nm gold particles
19 Passive Adsorption Beads w/ com1 Aggregated antigen Gold particles
20 SUMMARY Bead technology is a rapid, functional approach for producing biosimulants for any biological agent Surface antigenicity of agent is mimicked Physical characteristics are customizable Produced two bead simulants for C. burnetii Recognized by both poly/monoclonal antibodies Levels of bead (com1) detection similar to Coxiella Distribution of com1 across bead populations similar to Coxiella Good surface antigen mimic of Coxiella
21 ACKNWLEDGEMENTS Dugway Proving Ground Life Sciences Division Richard Warren, Ph.D. Steven Burke, Ph.D. Dan Carmany Tana Robert Jim Bragg Texas A&M University Health Science Center Dept. Medical Microbiology & Immunology James Samuel, Ph.D.
22 Flow Cytometry of Polystyrene Beads Relative Size Distribution (FS) Coxiella Passive Adsorption Beads Covalent Coupling Beads Number Number Number FS LG FS LG FS LG Actual Size: 0.5 µm 9.9 µm 7.4 µm
23 Biological Point Detection Systems Real-time detection of biological agents (on-site) Components: Detector/Trigger (BAWS) Collector (Cyclone) Fluid transfer system Identifier (PCR, immunoassay) Field-tested with simulants Joint Biological Point Detection System (JBPDS)
24 Two approaches for antigen attachment: Passive adsorption Attachment due to hydrophobic interactions Positives: fast, easy, relatively inexpensive Negatives: temporary, less stable, antigen partially unfolded (still antigenic?) Covalent coupling Antigen attached via covalent bonding Positives: permanent, more stable, native (antigenic) Negatives: complex, more expensive procedure
25 Benefits of Bead Technology Fast, easy only requires agent-specific recombinant antigen Customize size and surface antigens Mimics physical AND detection properties Can be potentially used to simulate any biological agent: Bacteria B. anthracis Viruses VEE Toxins Ricin
26 Covalent Attachment of com1 to NH 2 -Modified Polystyrene Beads com1 Na 3 S N sulfo-smcc N Cross-link Polystyrene Bead NH 2 H Maleimide activated Polystyrene Bead NH com1 NH SH Com1 covalently attached to bead Polystyrene Bead NH HN Maleimide spacer (~15 Å) S Free SH groups N-succinimidyl S-acetylthioacetate (SATA) CH 3 Activation N S N H com1 Hydroxyl amine NH N H 2 S H CH 3 H Deacetylation NH CH 3 Bead 15 Å Ag com 1 NH 2
27 Summary--ELISA com1 attached to beads (adsorbed and covalent) Mono/polyclonal antibodies detect com1 on both bead preps similar to Coxiella Both bead preps sufficient Coxiella mimics (surface antigenicity)
28 Basic Components of Flow Cytometry Cell suspension Fluorescence detector Flow cell Dichroic mirror Digital Computer: Signal processor Digital conversion Side scatter Laser Lens (typ) Forward scatter Data storage Display
29 Flow Cytometry of Polystyrene Beads Relative Size Distribution (FS) Passive Adsorption Beads Covalent Coupling Beads Count Number Count Number FS LG FS LG
30 Flow Cytometry of Polystyrene Beads Relative Fluorescence (FL) Distribution Passive Adsorption Beads Covalent Coupling Beads Count Number Count Number FL1 LG FL1 LG
31 Flow Cytometry of Polystyrene Beads SS vs FS Passive Adsorption Beads Covalent Coupling Beads FL1 LG FL1 LG FS FS
32 Summary--Flow Cytometry Size distribution of passive adsorption beads is wider than Coxiella; covalent bead size distribution similar to Coxiella Controllable com1 binding (fluorescence) distribution is similar among both bead populations and Coxiella
33 Passive Adsorption Beads w/ com1
34 Coxiella burnetii No Antibody
35 Coxiella burnetii α com1 Monoclonal Antibody
36 Summary--Immuno SEM Uniform coating of com1 on covalent beads com1 clumped (aggregated) on passive adsorption beads Does not significantly affect com1 antigenicity
37 Poly and Monoclonal Antibody Affinity for com1 Antibody Antigen K D (M) Poly pdpg Coxiella 1.2E E E E-08 Poly pdpg com1 3.7E E E E-08 Mono 11B24 Coxiella 1.7E E-09 Mono 13A44 4.0E E E-09 Mono 11B24 com1 3.4E E-09 Mono 13A44 3.5E E E-09
38 Antibody Avidity/Affinity for C. burnetii and com1 Antibody Class Antigen K D (M) pdpg Polyclonal Coxiella 1.95E-08 Polyclonal com1 2.00E-08 11B24/13A44 Monoclonal Coxiella 2.24E-09 Monoclonal com1 3.68E-09 Purified com1 binds poly/monoclonal Ab similar to killed C. burnetii samples com1 is a good mimic for C. burnetii
39 Concentration of com1 on Polystyrene Beads Passive Adsorption Covalent Linkage Bound com1 (µg/ml) mg com1 Added to Beads
40 Three Channel Sensor (BAWS)
COM DEV AIS Initiative. TEXAS II Meeting September 03, 2008 Ian D Souza
COM DEV AIS Initiative TEXAS II Meeting September 03, 2008 Ian D Souza 1 Report Documentation Page Form Approved OMB No. 0704-0188 Public reporting burden for the collection of information is estimated
INTEGRATIVE MIGRATORY BIRD MANAGEMENT ON MILITARY BASES: THE ROLE OF RADAR ORNITHOLOGY
INTEGRATIVE MIGRATORY BIRD MANAGEMENT ON MILITARY BASES: THE ROLE OF RADAR ORNITHOLOGY Sidney A. Gauthreaux, Jr. and Carroll G. Belser Department of Biological Sciences Clemson University Clemson, SC 29634-0314
MATLAB Algorithms for Rapid Detection and Embedding of Palindrome and Emordnilap Electronic Watermarks in Simulated Chemical and Biological Image Data
MATLAB Algorithms for Rapid Detection and Embedding of Palindrome and Emordnilap Electronic Watermarks in Simulated Chemical and Biological Image Data Ronny C. Robbins Edgewood Chemical and Biological
Bistatic Underwater Optical Imaging Using AUVs
Bistatic Underwater Optical Imaging Using AUVs Michael P. Strand Naval Surface Warfare Center Panama City Code HS-12, 110 Vernon Avenue Panama City, FL 32407 phone: (850) 235-5457 fax: (850) 234-4867 email:
Signal Processing Architectures for Ultra-Wideband Wide-Angle Synthetic Aperture Radar Applications
Signal Processing Architectures for Ultra-Wideband Wide-Angle Synthetic Aperture Radar Applications Atindra Mitra Joe Germann John Nehrbass AFRL/SNRR SKY Computers ASC/HPC High Performance Embedded Computing
UNCLASSIFIED UNCLASSIFIED 1
UNCLASSIFIED 1 Report Documentation Page Form Approved OMB No. 0704-0188 Public reporting burden for the collection of information is estimated to average 1 hour per response, including the time for reviewing
Investigation of a Forward Looking Conformal Broadband Antenna for Airborne Wide Area Surveillance
Investigation of a Forward Looking Conformal Broadband Antenna for Airborne Wide Area Surveillance Hany E. Yacoub Department Of Electrical Engineering & Computer Science 121 Link Hall, Syracuse University,
Technology Maturation Planning for the Autonomous Approach and Landing Capability (AALC) Program
Technology Maturation Planning for the Autonomous Approach and Landing Capability (AALC) Program AFRL 2008 Technology Maturity Conference Multi-Dimensional Assessment of Technology Maturity 9-12 September
14. Model Based Systems Engineering: Issues of application to Soft Systems
DSTO-GD-0734 14. Model Based Systems Engineering: Issues of application to Soft Systems Ady James, Alan Smith and Michael Emes UCL Centre for Systems Engineering, Mullard Space Science Laboratory Abstract
A Comparison of Two Computational Technologies for Digital Pulse Compression
A Comparison of Two Computational Technologies for Digital Pulse Compression Presented by Michael J. Bonato Vice President of Engineering Catalina Research Inc. A Paravant Company High Performance Embedded
Robotics and Artificial Intelligence. Rodney Brooks Director, MIT Computer Science and Artificial Intelligence Laboratory CTO, irobot Corp
Robotics and Artificial Intelligence Rodney Brooks Director, MIT Computer Science and Artificial Intelligence Laboratory CTO, irobot Corp Report Documentation Page Form Approved OMB No. 0704-0188 Public
Strategic Technical Baselines for UK Nuclear Clean-up Programmes. Presented by Brian Ensor Strategy and Engineering Manager NDA
Strategic Technical Baselines for UK Nuclear Clean-up Programmes Presented by Brian Ensor Strategy and Engineering Manager NDA Report Documentation Page Form Approved OMB No. 0704-0188 Public reporting
Durable Aircraft. February 7, 2011
Durable Aircraft February 7, 2011 Report Documentation Page Form Approved OMB No. 0704-0188 Public reporting burden for the collection of information is estimated to average 1 hour per response, including
Frequency Stabilization Using Matched Fabry-Perots as References
April 1991 LIDS-P-2032 Frequency Stabilization Using Matched s as References Peter C. Li and Pierre A. Humblet Massachusetts Institute of Technology Laboratory for Information and Decision Systems Cambridge,
Underwater Intelligent Sensor Protection System
Underwater Intelligent Sensor Protection System Peter J. Stein, Armen Bahlavouni Scientific Solutions, Inc. 18 Clinton Drive Hollis, NH 03049-6576 Phone: (603) 880-3784, Fax: (603) 598-1803, email: pstein@mv.mv.com
Hybrid QR Factorization Algorithm for High Performance Computing Architectures. Peter Vouras Naval Research Laboratory Radar Division
Hybrid QR Factorization Algorithm for High Performance Computing Architectures Peter Vouras Naval Research Laboratory Radar Division 8/1/21 Professor G.G.L. Meyer Johns Hopkins University Parallel Computing
0.18 μm CMOS Fully Differential CTIA for a 32x16 ROIC for 3D Ladar Imaging Systems
0.18 μm CMOS Fully Differential CTIA for a 32x16 ROIC for 3D Ladar Imaging Systems Jirar Helou Jorge Garcia Fouad Kiamilev University of Delaware Newark, DE William Lawler Army Research Laboratory Adelphi,
Department of Defense Partners in Flight
Department of Defense Partners in Flight Conserving birds and their habitats on Department of Defense lands Chris Eberly, DoD Partners in Flight ceberly@dodpif.org DoD Conservation Conference Savannah
Method and Results Watermarking Materials. Introduction
MATLAB Algorithms for Rapid Detection and Embedding of Palindrome and Emordnilap Electronic Watermarks in Simulated Chemical and Biological Image Data Ronny C. Robbins Edgewood Chemical and Biological
Digital Radiography and X-ray Computed Tomography Slice Inspection of an Aluminum Truss Section
Digital Radiography and X-ray Computed Tomography Slice Inspection of an Aluminum Truss Section by William H. Green ARL-MR-791 September 2011 Approved for public release; distribution unlimited. NOTICES
Wavelength Division Multiplexing (WDM) Technology for Naval Air Applications
Wavelength Division Multiplexing (WDM) Technology for Naval Air Applications Drew Glista Naval Air Systems Command Patuxent River, MD glistaas@navair.navy.mil 301-342-2046 1 Report Documentation Page Form
REPORT DOCUMENTATION PAGE
REPORT DOCUMENTATION PAGE Form Approved OMB No. 0704-0188 Public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions,
Loop-Dipole Antenna Modeling using the FEKO code
Loop-Dipole Antenna Modeling using the FEKO code Wendy L. Lippincott* Thomas Pickard Randy Nichols lippincott@nrl.navy.mil, Naval Research Lab., Code 8122, Wash., DC 237 ABSTRACT A study was done to optimize
A HIGH-PRECISION COUNTER USING THE DSP TECHNIQUE
A HIGH-PRECISION COUNTER USING THE DSP TECHNIQUE Shang-Shian Chen, Po-Cheng Chang, Hsin-Min Peng, and Chia-Shu Liao Telecommunication Labs., Chunghwa Telecom No. 12, Lane 551, Min-Tsu Road Sec. 5 Yang-Mei,
Development of a charged-particle accumulator using an RF confinement method FA
Development of a charged-particle accumulator using an RF confinement method FA4869-08-1-4075 Ryugo S. Hayano, University of Tokyo 1 Impact of the LHC accident This project, development of a charged-particle
Cross-layer Approach to Low Energy Wireless Ad Hoc Networks
Cross-layer Approach to Low Energy Wireless Ad Hoc Networks By Geethapriya Thamilarasu Dept. of Computer Science & Engineering, University at Buffalo, Buffalo NY Dr. Sumita Mishra CompSys Technologies,
A Multi-Use Low-Cost, Integrated, Conductivity/Temperature Sensor
A Multi-Use Low-Cost, Integrated, Conductivity/Temperature Sensor Guy J. Farruggia Areté Associates 1725 Jefferson Davis Hwy Suite 703 Arlington, VA 22202 phone: (703) 413-0290 fax: (703) 413-0295 email:
Management of Toxic Materials in DoD: The Emerging Contaminants Program
SERDP/ESTCP Workshop Carole.LeBlanc@osd.mil Surface Finishing and Repair Issues 703.604.1934 for Sustaining New Military Aircraft February 26-28, 2008, Tempe, Arizona Management of Toxic Materials in DoD:
Innovative 3D Visualization of Electro-optic Data for MCM
Innovative 3D Visualization of Electro-optic Data for MCM James C. Luby, Ph.D., Applied Physics Laboratory University of Washington 1013 NE 40 th Street Seattle, Washington 98105-6698 Telephone: 206-543-6854
DISTRIBUTION A: Distribution approved for public release.
AFRL-OSR-VA-TR-2014-0205 Optical Materials PARAS PRASAD RESEARCH FOUNDATION OF STATE UNIVERSITY OF NEW YORK THE 05/30/2014 Final Report DISTRIBUTION A: Distribution approved for public release. Air Force
REPORT DOCUMENTATION PAGE
REPORT DOCUMENTATION PAGE Form Approved OMB No. 0704-0188 Public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions,
Lattice Spacing Effect on Scan Loss for Bat-Wing Phased Array Antennas
Lattice Spacing Effect on Scan Loss for Bat-Wing Phased Array Antennas I. Introduction Thinh Q. Ho*, Charles A. Hewett, Lilton N. Hunt SSCSD 2825, San Diego, CA 92152 Thomas G. Ready NAVSEA PMS500, Washington,
Design of Synchronization Sequences in a MIMO Demonstration System 1
Design of Synchronization Sequences in a MIMO Demonstration System 1 Guangqi Yang,Wei Hong,Haiming Wang,Nianzu Zhang State Key Lab. of Millimeter Waves, Dept. of Radio Engineering, Southeast University,
Report Documentation Page
Report Documentation Page Form Approved OMB No. 0704-0188 Public reporting burden for the collection of information is estimated to average 1 hour per response, including the time for reviewing instructions,
FAA Research and Development Efforts in SHM
FAA Research and Development Efforts in SHM P. SWINDELL and D. P. ROACH ABSTRACT SHM systems are being developed using networks of sensors for the continuous monitoring, inspection and damage detection
REPORT DOCUMENTATION PAGE
REPORT DOCUMENTATION PAGE Form Approved OMB No. 0704-0188 Public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions,
DARPA TRUST in IC s Effort. Dr. Dean Collins Deputy Director, MTO 7 March 2007
DARPA TRUST in IC s Effort Dr. Dean Collins Deputy Director, MTO 7 March 27 Report Documentation Page Form Approved OMB No. 74-88 Public reporting burden for the collection of information is estimated
Drexel Object Occlusion Repository (DOOR) Trip Denton, John Novatnack and Ali Shokoufandeh
Drexel Object Occlusion Repository (DOOR) Trip Denton, John Novatnack and Ali Shokoufandeh Technical Report DU-CS-05-08 Department of Computer Science Drexel University Philadelphia, PA 19104 July, 2005
USAARL NUH-60FS Acoustic Characterization
USAARL Report No. 2017-06 USAARL NUH-60FS Acoustic Characterization By Michael Chen 1,2, J. Trevor McEntire 1,3, Miles Garwood 1,3 1 U.S. Army Aeromedical Research Laboratory 2 Laulima Government Solutions,
Report Documentation Page
Svetlana Avramov-Zamurovic 1, Bryan Waltrip 2 and Andrew Koffman 2 1 United States Naval Academy, Weapons and Systems Engineering Department Annapolis, MD 21402, Telephone: 410 293 6124 Email: avramov@usna.edu
August 9, Attached please find the progress report for ONR Contract N C-0230 for the period of January 20, 2015 to April 19, 2015.
August 9, 2015 Dr. Robert Headrick ONR Code: 332 O ce of Naval Research 875 North Randolph Street Arlington, VA 22203-1995 Dear Dr. Headrick, Attached please find the progress report for ONR Contract N00014-14-C-0230
Adaptive CFAR Performance Prediction in an Uncertain Environment
Adaptive CFAR Performance Prediction in an Uncertain Environment Jeffrey Krolik Department of Electrical and Computer Engineering Duke University Durham, NC 27708 phone: (99) 660-5274 fax: (99) 660-5293
CFDTD Solution For Large Waveguide Slot Arrays
I. Introduction CFDTD Solution For Large Waveguide Slot Arrays T. Q. Ho*, C. A. Hewett, L. N. Hunt SSCSD 2825, San Diego, CA 92152 T. G. Ready NAVSEA PMS5, Washington, DC 2376 M. C. Baugher, K. E. Mikoleit
A RENEWED SPIRIT OF DISCOVERY
A RENEWED SPIRIT OF DISCOVERY The President s Vision for U.S. Space Exploration PRESIDENT GEORGE W. BUSH JANUARY 2004 Report Documentation Page Form Approved OMB No. 0704-0188 Public reporting burden for
Fuzzy Logic Approach for Impact Source Identification in Ceramic Plates
Fuzzy Logic Approach for Impact Source Identification in Ceramic Plates Shashank Kamthan 1, Harpreet Singh 1, Arati M. Dixit 1, Vijay Shrama 1, Thomas Reynolds 2, Ivan Wong 2, Thomas Meitzler 2 1 Dept
REPORT DOCUMENTATION PAGE
REPORT DOCUMENTATION PAGE Form Approved OMB NO. 0704-0188 The public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions,
INFRARED REFLECTANCE INSPECTION
Infrared Reflectance Imaging for Corrosion Inspection Through Organic Coatings (WP-0407) Mr. Jack Benfer Principal Investigator NAVAIR Jacksonville, FL Tel: (904) 542-4516, x153 Email: john.benfer@navy.mil
REPORT DOCUMENTATION PAGE. Thermal transport and measurement of specific heat in artificially sculpted nanostructures. Dr. Mandar Madhokar Deshmukh
REPORT DOCUMENTATION PAGE Form Approved OMB No. 0704-0188 The public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions,
Combining High Dynamic Range Photography and High Range Resolution RADAR for Pre-discharge Threat Cues
Combining High Dynamic Range Photography and High Range Resolution RADAR for Pre-discharge Threat Cues Nikola Subotic Nikola.Subotic@mtu.edu DISTRIBUTION STATEMENT A. Approved for public release; distribution
Modeling an HF NVIS Towel-Bar Antenna on a Coast Guard Patrol Boat A Comparison of WIPL-D and the Numerical Electromagnetics Code (NEC)
Modeling an HF NVIS Towel-Bar Antenna on a Coast Guard Patrol Boat A Comparison of WIPL-D and the Numerical Electromagnetics Code (NEC) Darla Mora, Christopher Weiser and Michael McKaughan United States
PULSED POWER SWITCHING OF 4H-SIC VERTICAL D-MOSFET AND DEVICE CHARACTERIZATION
PULSED POWER SWITCHING OF 4H-SIC VERTICAL D-MOSFET AND DEVICE CHARACTERIZATION Argenis Bilbao, William B. Ray II, James A. Schrock, Kevin Lawson and Stephen B. Bayne Texas Tech University, Electrical and
THE DET CURVE IN ASSESSMENT OF DETECTION TASK PERFORMANCE
THE DET CURVE IN ASSESSMENT OF DETECTION TASK PERFORMANCE A. Martin*, G. Doddington#, T. Kamm+, M. Ordowski+, M. Przybocki* *National Institute of Standards and Technology, Bldg. 225-Rm. A216, Gaithersburg,
Modeling and Evaluation of Bi-Static Tracking In Very Shallow Water
Modeling and Evaluation of Bi-Static Tracking In Very Shallow Water Stewart A.L. Glegg Dept. of Ocean Engineering Florida Atlantic University Boca Raton, FL 33431 Tel: (954) 924 7241 Fax: (954) 924-7270
Mathematics, Information, and Life Sciences
Mathematics, Information, and Life Sciences 05 03 2012 Integrity Service Excellence Dr. Hugh C. De Long Interim Director, RSL Air Force Office of Scientific Research Air Force Research Laboratory 15 February
U.S. Army Training and Doctrine Command (TRADOC) Virtual World Project
U.S. Army Research, Development and Engineering Command U.S. Army Training and Doctrine Command (TRADOC) Virtual World Project Advanced Distributed Learning Co-Laboratory ImplementationFest 2010 12 August
IREAP. MURI 2001 Review. John Rodgers, T. M. Firestone,V. L. Granatstein, M. Walter
MURI 2001 Review Experimental Study of EMP Upset Mechanisms in Analog and Digital Circuits John Rodgers, T. M. Firestone,V. L. Granatstein, M. Walter Institute for Research in Electronics and Applied Physics
DoDTechipedia. Technology Awareness. Technology and the Modern World
DoDTechipedia Technology Awareness Defense Technical Information Center Christopher Thomas Chief Technology Officer cthomas@dtic.mil 703-767-9124 Approved for Public Release U.S. Government Work (17 USC
ADVANCED CONTROL FILTERING AND PREDICTION FOR PHASED ARRAYS IN DIRECTED ENERGY SYSTEMS
AFRL-RD-PS- TR-2014-0036 AFRL-RD-PS- TR-2014-0036 ADVANCED CONTROL FILTERING AND PREDICTION FOR PHASED ARRAYS IN DIRECTED ENERGY SYSTEMS James Steve Gibson University of California, Los Angeles Office
Active Denial Array. Directed Energy. Technology, Modeling, and Assessment
Directed Energy Technology, Modeling, and Assessment Active Denial Array By Randy Woods and Matthew Ketner 70 Active Denial Technology (ADT) which encompasses the use of millimeter waves as a directed-energy,
REPORT DOCUMENTATION PAGE
REPORT DOCUMENTATION PAGE Form Approved OMB No. 0704-0188 Public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions,
Coherent distributed radar for highresolution
. Calhoun Drive, Suite Rockville, Maryland, 8 () 9 http://www.i-a-i.com Intelligent Automation Incorporated Coherent distributed radar for highresolution through-wall imaging Progress Report Contract No.
Experiences Linking Vehicle Motion Simulators to Distributed Simulation Experiments
Experiences Linking Vehicle Motion Simulators to Distributed Simulation Experiments Richard W. Jacobson Electrical Engineer 1/ 18 Report Documentation Page Form Approved OMB No. 0704-0188 Public reporting
Feasibility of T/R Module Functionality in a Single SiGe IC
Feasibility of T/R Module Functionality in a Single SiGe IC Dr. John D. Cressler, Jonathan Comeau, Joel Andrews, Lance Kuo, Matt Morton, and Dr. John Papapolymerou Georgia Institute of Technology Georgia
Modeling of Ionospheric Refraction of UHF Radar Signals at High Latitudes
Modeling of Ionospheric Refraction of UHF Radar Signals at High Latitudes Brenton Watkins Geophysical Institute University of Alaska Fairbanks USA watkins@gi.alaska.edu Sergei Maurits and Anton Kulchitsky
RADAR SATELLITES AND MARITIME DOMAIN AWARENESS
RADAR SATELLITES AND MARITIME DOMAIN AWARENESS J.K.E. Tunaley Corporation, 114 Margaret Anne Drive, Ottawa, Ontario K0A 1L0 (613) 839-7943 Report Documentation Page Form Approved OMB No. 0704-0188 Public
3. Faster, Better, Cheaper The Fallacy of MBSE?
DSTO-GD-0734 3. Faster, Better, Cheaper The Fallacy of MBSE? Abstract David Long Vitech Corporation Scope, time, and cost the three fundamental constraints of a project. Project management theory holds
POSTPRINT UNITED STATES AIR FORCE RESEARCH ON AIRFIELD PAVEMENT REPAIRS USING PRECAST PORTLAND CEMENT CONCRETE (PCC) SLABS (BRIEFING SLIDES)
POSTPRINT AFRL-RX-TY-TP-2008-4582 UNITED STATES AIR FORCE RESEARCH ON AIRFIELD PAVEMENT REPAIRS USING PRECAST PORTLAND CEMENT CONCRETE (PCC) SLABS (BRIEFING SLIDES) Athar Saeed, PhD, PE Applied Research
Acoustic Change Detection Using Sources of Opportunity
Acoustic Change Detection Using Sources of Opportunity by Owen R. Wolfe and Geoffrey H. Goldman ARL-TN-0454 September 2011 Approved for public release; distribution unlimited. NOTICES Disclaimers The findings
LONG TERM GOALS OBJECTIVES
A PASSIVE SONAR FOR UUV SURVEILLANCE TASKS Stewart A.L. Glegg Dept. of Ocean Engineering Florida Atlantic University Boca Raton, FL 33431 Tel: (561) 367-2633 Fax: (561) 367-3885 e-mail: glegg@oe.fau.edu
Limits to the Exponential Advances in DWDM Filter Technology? Philip J. Anthony
Limits to the Exponential Advances in DWDM Filter Technology? DARPA/MTO WDM for Military Platforms April 18-19, 2000 McLean, VA Philip J. Anthony E-TEK Dynamics San Jose CA phil.anthony@e-tek.com Report
DIELECTRIC ROTMAN LENS ALTERNATIVES FOR BROADBAND MULTIPLE BEAM ANTENNAS IN MULTI-FUNCTION RF APPLICATIONS. O. Kilic U.S. Army Research Laboratory
DIELECTRIC ROTMAN LENS ALTERNATIVES FOR BROADBAND MULTIPLE BEAM ANTENNAS IN MULTI-FUNCTION RF APPLICATIONS O. Kilic U.S. Army Research Laboratory ABSTRACT The U.S. Army Research Laboratory (ARL) is currently
HIGH TEMPERATURE (250 C) SIC POWER MODULE FOR MILITARY HYBRID ELECTRICAL VEHICLE APPLICATIONS
HIGH TEMPERATURE (250 C) SIC POWER MODULE FOR MILITARY HYBRID ELECTRICAL VEHICLE APPLICATIONS R. M. Schupbach, B. McPherson, T. McNutt, A. B. Lostetter John P. Kajs, and Scott G Castagno 29 July 2011 :
Social Science: Disciplined Study of the Social World
Social Science: Disciplined Study of the Social World Elisa Jayne Bienenstock MORS Mini-Symposium Social Science Underpinnings of Complex Operations (SSUCO) 18-21 October 2010 Report Documentation Page
Acoustic Measurements of Tiny Optically Active Bubbles in the Upper Ocean
Acoustic Measurements of Tiny Optically Active Bubbles in the Upper Ocean Svein Vagle Ocean Sciences Division Institute of Ocean Sciences 9860 West Saanich Road P.O. Box 6000 Sidney, BC, V8L 4B2 Canada
FY07 New Start Program Execution Strategy
FY07 New Start Program Execution Strategy DISTRIBUTION STATEMENT D. Distribution authorized to the Department of Defense and U.S. DoD contractors strictly associated with TARDEC for the purpose of providing
NEURAL NETWORKS IN ANTENNA ENGINEERING BEYOND BLACK-BOX MODELING
NEURAL NETWORKS IN ANTENNA ENGINEERING BEYOND BLACK-BOX MODELING Amalendu Patnaik 1, Dimitrios Anagnostou 2, * Christos G. Christodoulou 2 1 Electronics and Communication Engineering Department National
Effects of Fiberglass Poles on Radiation Patterns of Log-Periodic Antennas
Effects of Fiberglass Poles on Radiation Patterns of Log-Periodic Antennas by Christos E. Maragoudakis ARL-TN-0357 July 2009 Approved for public release; distribution is unlimited. NOTICES Disclaimers
David Siegel Masters Student University of Cincinnati. IAB 17, May 5 7, 2009 Ford & UM
Alternator Health Monitoring For Vehicle Applications David Siegel Masters Student University of Cincinnati Report Documentation Page Form Approved OMB No. 0704-0188 Public reporting burden for the collection
CATALOG & PRICE. Magnetic Beads Products
CATALOG & PRICE Magnetic Beads Products 2017 Page 1 of 9 Table of Contents PROTEIN PURIFICATION MAGNETIC BEADS...4 STREPTAVIDIN MAGNETIC BEADS...4 PROTEIN A MAGNETIC BEADS...4 GLUTATHIONE (GST-TAG AFFINITY)
Multiplexing as Essential Tool for Modern Biology
Multiplexing as Essential Tool for Modern Biology Bio-Plex Seminar, Debrecen, 2012. Gyula Csanádi, PhD. The "Age of "-omics" Studying interrelationships at different level of complexity Genes - Unveiling
Counter-Terrorism Initiatives in Defence R&D Canada. Rod Schmitke Canadian Embassy, Washington NDIA Conference 26 February 2002
Counter-Terrorism Initiatives in Rod Schmitke Canadian Embassy, Washington NDIA Conference 26 February 2002 Report Documentation Page Form Approved OMB No. 0704-0188 Public reporting burden for the collection
SILICON CARBIDE FOR NEXT GENERATION VEHICULAR POWER CONVERTERS. John Kajs SAIC August UNCLASSIFIED: Dist A. Approved for public release
SILICON CARBIDE FOR NEXT GENERATION VEHICULAR POWER CONVERTERS John Kajs SAIC 18 12 August 2010 Report Documentation Page Form Approved OMB No. 0704-0188 Public reporting burden for the collection of information
CONTROL OF SENSORS FOR SEQUENTIAL DETECTION A STOCHASTIC APPROACH
file://\\52zhtv-fs-725v\cstemp\adlib\input\wr_export_131127111121_237836102... Page 1 of 1 11/27/2013 AFRL-OSR-VA-TR-2013-0604 CONTROL OF SENSORS FOR SEQUENTIAL DETECTION A STOCHASTIC APPROACH VIJAY GUPTA
Effects of Radar Absorbing Material (RAM) on the Radiated Power of Monopoles with Finite Ground Plane
Effects of Radar Absorbing Material (RAM) on the Radiated Power of Monopoles with Finite Ground Plane by Christos E. Maragoudakis and Vernon Kopsa ARL-TN-0340 January 2009 Approved for public release;
Thermal Simulation of a Silicon Carbide (SiC) Insulated-Gate Bipolar Transistor (IGBT) in Continuous Switching Mode
ARL-MR-0973 APR 2018 US Army Research Laboratory Thermal Simulation of a Silicon Carbide (SiC) Insulated-Gate Bipolar Transistor (IGBT) in Continuous Switching Mode by Gregory Ovrebo NOTICES Disclaimers
Ocean Acoustics and Signal Processing for Robust Detection and Estimation
Ocean Acoustics and Signal Processing for Robust Detection and Estimation Zoi-Heleni Michalopoulou Department of Mathematical Sciences New Jersey Institute of Technology Newark, NJ 07102 phone: (973) 596
REPORT DOCUMENTATION PAGE
REPORT DOCUMENTATION PAGE Form Approved OMB No. 0704-0188 Public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions,
Radar Detection of Marine Mammals
DISTRIBUTION STATEMENT A. Approved for public release; distribution is unlimited. Radar Detection of Marine Mammals Charles P. Forsyth Areté Associates 1550 Crystal Drive, Suite 703 Arlington, VA 22202
Rump Session: Advanced Silicon Technology Foundry Access Options for DoD Research. Prof. Ken Shepard. Columbia University
Rump Session: Advanced Silicon Technology Foundry Access Options for DoD Research Prof. Ken Shepard Columbia University The views and opinions presented by the invited speakers are their own and should
Department of Energy Technology Readiness Assessments Process Guide and Training Plan
Department of Energy Technology Readiness Assessments Process Guide and Training Plan Steven Krahn, Kurt Gerdes Herbert Sutter Department of Energy Consultant, Department of Energy 2008 Technology Maturity
Evanescent Acoustic Wave Scattering by Targets and Diffraction by Ripples
Evanescent Acoustic Wave Scattering by Targets and Diffraction by Ripples PI name: Philip L. Marston Physics Department, Washington State University, Pullman, WA 99164-2814 Phone: (509) 335-5343 Fax: (509)
Remote Sediment Property From Chirp Data Collected During ASIAEX
Remote Sediment Property From Chirp Data Collected During ASIAEX Steven G. Schock Department of Ocean Engineering Florida Atlantic University Boca Raton, Fl. 33431-0991 phone: 561-297-3442 fax: 561-297-3885
Ultrasonic Nonlinearity Parameter Analysis Technique for Remaining Life Prediction
Ultrasonic Nonlinearity Parameter Analysis Technique for Remaining Life Prediction by Raymond E Brennan ARL-TN-0636 September 2014 Approved for public release; distribution is unlimited. NOTICES Disclaimers
Presentation to TEXAS II
Presentation to TEXAS II Technical exchange on AIS via Satellite II Dr. Dino Lorenzini Mr. Mark Kanawati September 3, 2008 3554 Chain Bridge Road Suite 103 Fairfax, Virginia 22030 703-273-7010 1 Report
BIOGRAPHY ABSTRACT. This paper will present the design of the dual-frequency L1/L2 S-CRPA and the measurement results of the antenna elements.
Test Results of a Dual Frequency (L1/L2) Small Controlled Reception Pattern Antenna Huan-Wan Tseng, Randy Kurtz, Alison Brown, NAVSYS Corporation; Dean Nathans, Francis Pahr, SPAWAR Systems Center, San
Best Practices for Technology Transition. Technology Maturity Conference September 12, 2007
Best Practices for Technology Transition Technology Maturity Conference September 12, 2007 1 Report Documentation Page Form Approved OMB No. 0704-0188 Public reporting burden for the collection of information
SA Joint USN/USMC Spectrum Conference. Gerry Fitzgerald. Organization: G036 Project: 0710V250-A1
SA2 101 Joint USN/USMC Spectrum Conference Gerry Fitzgerald 04 MAR 2010 DISTRIBUTION A: Approved for public release Case 10-0907 Organization: G036 Project: 0710V250-A1 Report Documentation Page Form Approved
Key Issues in Modulating Retroreflector Technology
Key Issues in Modulating Retroreflector Technology Dr. G. Charmaine Gilbreath, Code 7120 Naval Research Laboratory 4555 Overlook Ave., NW Washington, DC 20375 phone: (202) 767-0170 fax: (202) 404-8894
The Energy Spectrum of Accelerated Electrons from Waveplasma Interactions in the Ionosphere
AFRL-AFOSR-UK-TR-2012-0014 The Energy Spectrum of Accelerated Electrons from Waveplasma Interactions in the Ionosphere Mike J. Kosch Physics Department Bailrigg Lancaster, United Kingdom LA1 4YB EOARD
Noise Tolerance of Improved Max-min Scanning Method for Phase Determination
Noise Tolerance of Improved Max-min Scanning Method for Phase Determination Xu Ding Research Assistant Mechanical Engineering Dept., Michigan State University, East Lansing, MI, 48824, USA Gary L. Cloud,