MT-540. D-Ribavirin, [ 3 H]- Lot A DG
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2 MT-54 D-Ribavirin, [ 3 H]- Lot A-2835-DG A) All chromatograms were run using the HPLC method described on the Product Data Sheet. Concentrations and volumes: Standard solution concentration was.5 mg/ml. D-Ribavirin, [ 3 H]- concentration was.1 mci/ml. Volume of standard alone injection was 2. µl. Volume of D-Ribavirin, [ 3 H]- alone injection was 2. µl. Co-injection solution consisted of 2. µl D-Ribavirin, [ 3 H] µl standard. Volume of co-injection was 4. µl. Volume of blank injection was 2. µl. B) Mass spectrometry Positive mode C) NMR
3 2/24/214 8:52 AM Chromatogram L:\int2457.PRM Page 1 of 2 Chromatogram Info: MT-54 D-Ribavirin, [3H]- Lot A-2835-DG File Name : L:\int2457 File Created : 2/24/214 8:4:15 AM Origin : Acquired, Acquisition started 3/3/21 1:36:2 PM Acquired Date : 3/3/21 1:56:18 PM Project : Test By : Administrator Method : unit2-2minrun By : Administrator Description : Radiochemical trace of 3H material alone Created : 8/8/27 9:12 AM Modified : 2/24/214 8:51 AM Column : Detection : Radiochemical Mobile Phase : Temperature : Flow Rate : Pressure : Note : L:\ int Unit 2 Radio Voltage Time [min.]
4 2/24/214 8:52 AM Chromatogram L:\int2457.PRM Page 2 of 2 Compound Name Reten. Time [min] [mv.s] Result Table (Uncal - L:\int Unit 2 Radio) Efficiency [th.pl] Eff/l [t.p./m] Symmetry/Taili ng Response Factor Resolution Total
5 2/24/214 8:51 AM Chromatogram L:\int2457.PRM Page 1 of 2 Chromatogram Info: MT-54 D-Ribavirin, [3H]- Lot A-2835-DG File Name : L:\int2457 File Created : 2/24/214 8:4:15 AM Origin : Acquired, Acquisition started 3/3/21 1:36:2 PM Acquired Date : 3/3/21 1:56:18 PM Project : Test By : Administrator Method : unit2-2minrun By : Administrator Description : UV trace of 3H material alone Created : 8/8/27 9:12 AM Modified : 2/24/214 8:5 AM Column : Detection : UV 212nm Mobile Phase : Temperature : Flow Rate : Pressure : Note : 1 L:\ int Unit 2 UV Voltage Time [min.]
6 2/24/214 8:51 AM Chromatogram L:\int2457.PRM Page 2 of 2 Compound Name Reten. Time [min] [mv.s] Result Table (Uncal - L:\int Unit 2 UV) Efficiency [th.pl] Eff/l [t.p./m] Symmetry/Taili ng Response Factor Resolution Total
7 2/24/214 8:53 AM Chromatogram L:\int2458.PRM Page 1 of 2 Chromatogram Info: MT-54 D-Ribavirin, [3H]- Lot A-2835-DG File Name : L:\int2458 File Created : 2/24/214 8:4:15 AM Origin : Acquired, Acquisition started 3/3/21 2:12:52 PM Acquired Date : 3/3/21 2:32:5 PM Project : Test By : Administrator Method : unit2-2minrun By : Administrator Description : UV trace of standard material alone Created : 8/8/27 9:12 AM Modified : 2/24/214 8:53 AM Column : Detection : UV 212nm Mobile Phase : Temperature : Flow Rate : Pressure : Note : L:\ int Unit 2 UV 2 Voltage Time [min.]
8 2/24/214 8:53 AM Chromatogram L:\int2458.PRM Page 2 of 2 Compound Name Reten. Time [min] [mv.s] Result Table (Uncal - L:\int Unit 2 UV) Efficiency [th.pl] Eff/l [t.p./m] Symmetry/Taili ng Total Response Factor Resolution
9 2/24/214 8:56 AM Chromatogram L:\int2459.PRM Page 1 of 2 Chromatogram Info: MT-54 D-Ribavirin, [3H]- Lot A-2835-DG File Name : L:\int2459 File Created : 2/24/214 8:4:15 AM Origin : Acquired, Acquisition started 3/3/21 2:38:4 PM Acquired Date : 3/3/21 2:58:3 PM Project : Test By : Administrator Method : unit2-2minrun By : Administrator Description : Radiochemical trace of 3H material co-injected with standard Created : 8/8/27 9:12 AM Modified : 2/24/214 8:55 AM Column : Detection : Radiochemical Mobile Phase : Temperature : Flow Rate : Pressure : Note : L:\ int Unit 2 Radio 5 4 Voltage Time [min.]
10 2/24/214 8:56 AM Chromatogram L:\int2459.PRM Page 2 of 2 Compound Name Reten. Time [min] [mv.s] Result Table (Uncal - L:\int Unit 2 Radio) Efficiency [th.pl] Eff/l [t.p./m] Symmetry/Taili ng Response Factor Resolution Total
11 2/24/214 8:54 AM Chromatogram L:\int2459.PRM Page 1 of 2 Chromatogram Info: MT-54 D-Ribavirin, [3H]- Lot A-2835-DG File Name : L:\int2459 File Created : 2/24/214 8:4:15 AM Origin : Acquired, Acquisition started 3/3/21 2:38:4 PM Acquired Date : 3/3/21 2:58:3 PM Project : Test By : Administrator Method : unit2-2minrun By : Administrator Description : UV trace of 3H material co-injected with standard Created : 8/8/27 9:12 AM Modified : 2/24/214 8:54 AM Column : Detection : UV 212nm Mobile Phase : Temperature : Flow Rate : Pressure : Note : L:\ int Unit 2 UV Voltage Time [min.]
12 2/24/214 8:54 AM Chromatogram L:\int2459.PRM Page 2 of 2 Compound Name Reten. Time [min] [mv.s] Result Table (Uncal - L:\int Unit 2 UV) Efficiency [th.pl] Eff/l [t.p./m] Symmetry/Taili ng Response Factor Resolution Total
13 2/24/214 8:56 AM Chromatogram L:\int2455.PRM Page 1 of 2 Chromatogram Info: MT-54 D-Ribavirin, [3H]- Lot A-2835-DG File Name : L:\int2455 File Created : 2/24/214 8:4:15 AM Origin : Acquired, Acquisition started 3/3/21 12:33:35 PM Acquired Date : 3/3/21 12:53:33 PM Project : Test By : Administrator Method : unit2-2minrun By : Administrator Description : UV trace of blank injection Created : 8/8/27 9:12 AM Modified : 2/24/214 8:56 AM Column : Detection : UV 212nm Mobile Phase : Temperature : Flow Rate : Pressure : Note : L:\ int Unit 2 UV 1 Voltage Time [min.]
14 2/24/214 8:56 AM Chromatogram L:\int2455.PRM Page 2 of 2 Result Table (Uncal - L:\int Unit 2 UV) Compound Name Reten. Time [min] [mv.s] No peak to report Efficiency [th.pl] Eff/l [t.p./m] Symmetry/Taili ng Response Factor Resolution
15 C:\Xcalibur\data\MT DG 8/23/21 9:3:43 AM MT DG RT: Relative Abundance NL: 2.6E8 TIC MS MT DG MT DG#1-184 RT: AV: T: + c NSI Full ms [5.-6.] m/z= m/z Intensity Relative Time (min) MT DG #1-184 RT: AV: 184 NL: 4.6E7 T: + c NSI Full ms [5.-6.] Relative Abundance m/z MT DG #1-184 RT: AV: 184 NL: 1.29E7 T: + c NSI Full ms [5.-6.] Relative Abundance m/z
16 Current Data Parameters NAME MT54 EXPNO 1 PROCNO 1 F2 Acquisition Parameters Date_ Time.57 INSTRUM spect PROBHD 5 mm DUX 3H 1H PULPROG zgdc TD SOLVENT MeOD NS 1 DS 2 SWH Hz FIDRES Hz AQ sec RG DW 81. usec DE 6. usec TE 3. K D1 2. sec d11.3 sec TD 1 ======== CHANNEL f1 ======== NUC1 3H P1 1. usec PL1 2. db SFO MHz ======== CHANNEL f2 ======== CPDPRG2 waltz16 NUC2 1H PCPD2 85. usec PL db PL2. db SFO MHz MT54 3H NMR in MeOD Batch 2835 DG ppm F2 Processing parameters SI SF MHz WDW no SSB LB. Hz GB PC 1. 1.
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