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11 READ THIS FIRST Changes to the Manual and Online Help Changes to the Manual and Online Help 7RVXJJHVWFKDQJHVWRWKLVPDQXDORUWKHRQOLQH+HOSSOHDVHVHQG \RXUFRPPHQWVWR (GLWRU7HFKQLFDO3XEOLFDWLRQV )LQQLJDQ&RUSRUDWLRQ 5LYHU2DNV3DUNZD\ 6DQ-RVH&$ <RXDUHHQFRXUDJHGWRUHSRUWHUURUVRURPLVVLRQVLQWKHWH[WDQG LQGH[7KDQN\RX xi Finnigan: LCQ MS Detector Hardware Manual

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igure 1-1. Finnigan LCQ system 7KH/&406GHWHFWRUFRQVLVWVRIDQDWPRVSKHULFSUHVVXUH LRQL]DWLRQ$3,VRXUFHLRQRSWLFVPDVVDQDO\]HUDQGLRQ GHWHFWLRQV\VWHP7KHLRQRSWLFVPDVVDQDO\]HULRQGHWHFWLRQ V\VWHPDQGSDUWRIWKH$3,VRXUFHDUHHQFORVHGLQDYDFXXP PDQLIROG,RQL]DWLRQRIWKHVDPSOHWDNHVSODFHLQWKH$3,VRXUFH 7KHVSHFLILFSURFHVVXVHGWRLRQL]HWKHVDPSOHLVUHIHUUHGWRDVWKH LRQL]DWLRQPRGH7KHLRQVSURGXFHGLQWKH$3,VRXUFHDUH Finnigan: LCQ MS Detector Hardware Manual 1-1

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innigan: LCQ MS Detector Hardware Manual

33 INTRODUCTION Ion Polarity Modes 1.1 Ion Polarity Modes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innigan: LCQ MS Detector Hardware Manual 1-3

34 INTRODUCTION Ionization Modes 1.2 Ionization Modes <RXFDQRSHUDWHWKH/&4LQHLWKHURIWZRLRQL]DWLRQPRGHVDV IROORZV (OHFWURVSUD\LRQL]DWLRQ(6, $WPRVSKHULFSUHVVXUHFKHPLFDOLRQL]DWLRQ$3&, Electrospray Ionization 7KHHOHFWURVSUD\LRQL]DWLRQ(6,PRGHWUDQVIRUPVLRQVLQVROXWLRQ LQWRLRQVLQWKHJDVSKDVH 0DQ\VDPSOHVWKDWSUHYLRXVO\ZHUH QRWVXLWDEOHIRUPDVVDQDO\VLVIRUH[DPSOHKHDWODELOHFRPSRXQGV RUKLJKPROHFXODUZHLJKWFRPSRXQGVFDQEHDQDO\]HGE\WKHXVH RI(6,(6,FDQEHXVHGWRDQDO\]HDQ\SRODUFRPSRXQGWKDW PDNHVDSUHIRUPHGLRQLQVROXWLRQ7KHWHUPSUHIRUPHGLRQFDQ LQFOXGHDGGXFWLRQV)RUH[DPSOHSRO\HWK\OHQHJO\FROVFDQEH DQDO\]HGIURPDVROXWLRQFRQWDLQLQJDPPRQLXPDFHWDWHEHFDXVH RIDGGXFWIRUPDWLRQEHWZHHQWKH1+ 4 LRQVLQWKHVROXWLRQDQG R[\JHQDWRPVLQWKHSRO\PHU:LWK(6,WKHUDQJHRIPROHFXODU ZHLJKWVWKDWFDQEHDQDO\]HGE\WKH/&4LVJUHDWHUWKDQ XGXHWRPXOWLSOHFKDUJLQJ(6,LVHVSHFLDOO\XVHIXOIRU WKHPDVVDQDO\VLVRISRODUFRPSRXQGVZKLFKLQFOXGHELRORJLFDO SRO\PHUVIRUH[DPSOHSURWHLQVSHSWLGHVJO\FRSURWHLQVDQG QXFOHRWLGHVSKDUPDFHXWLFDOVDQGWKHLUPHWDEROLWHVDQG LQGXVWULDOSRO\PHUVIRUH[DPSOHSRO\HWK\OHQHJO\FROV,Q(6,LRQVDUHSURGXFHGDQGDQDO\]HGDVIROORZV 1. The sample solution enters the ESI needle, to which a high voltage is applied. 2. The ESI needle sprays the sample solution into a fine mist of droplets that are electrically charged at their surface. 3. The electrical charge density at the surface of the droplets increases as solvent evaporates from the droplets. 5HIHUWRWKHIROORZLQJSDSHUVIRUPRUHLQIRUPDWLRQRQWKHHOHFWURVSUD\ LRQL]DWLRQSURFHVV)HQQ-%0DQQ00HQJ&.:RQJ6):KLWHKRXVH &00DVV6SHFWURP5HYLHZV6PLWK5'/RR-$(GPRQGV& *%DULQDJD&-8GVHWK+5$QDO&KHP,NRQRPRX0* %ODGHV$7.HEDUOH3$QDO&KHP 1-4 Finnigan: LCQ MS Detector Hardware Manual

35 INTRODUCTION Ionization Modes 4. The electrical charge density at the surface of the droplets increases to a critical point, known as the Rayleigh stability limit. At this critical point, the droplets divide into smaller droplets because the electrostatic repulsion is greater than the surface tension. The process is repeated many times to form very small droplets. 5. From the very small, highly charged droplets, sample ions are ejected into the gas phase by electrostatic repulsion. 6. The sample ions enter the MS detector and are analyzed. )LJXUHVKRZVWKHVWHSVLQWKHIRUPDWLRQRILRQVIURPKLJKO\ FKDUJHGGURSOHWV Figure 1-2. ESI process in the positive ion polarity mode Finnigan: LCQ MS Detector Hardware Manual 1-5

36 INTRODUCTION Ionization Modes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innigan: LCQ MS Detector Hardware Manual

37 INTRODUCTION Ionization Modes Atmospheric Pressure Chemical Ionization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innigan: LCQ MS Detector Hardware Manual 1-7

38 INTRODUCTION Ionization Modes Figure 1-3. APCI process in the positive ion polarity mode,qqhjdwlyhlrqprgh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innigan: LCQ MS Detector Hardware Manual

39 INTRODUCTION Ionization Modes 1RWH%HFDXVH$3&,DQG(6,XVHWKHVDPH$3,VWDFNWKDWLVWKH SRUWLRQRIWKH$3,VRXUFHWKDWLVXQGHUYDFXXP\RXFDQVZLWFK EHWZHHQWKHVHWZRLRQL]DWLRQWHFKQLTXHVLQMXVWDIHZPLQXWHV 6ZLWFKLQJLRQL]DWLRQPRGHVPHUHO\LQYROYHVVZLWFKLQJWKHSUREHV DQGGRHVQRWEUHDNYDFXXP Finnigan: LCQ MS Detector Hardware Manual 1-9

40 INTRODUCTION Scan Power 1.3 Scan Power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can $Q06RUPDVVVSHFWURPHWU\VFDQFRUUHVSRQGVWRDVLQJOHVWDJH RIPDVVDQDO\VLVWKDWLVDVFDQSRZHURIQ $Q06VFDQ LQYROYHVRQO\SDUHQWLRQVDQGQRIUDJPHQWDWLRQRIWKHSDUHQWLRQV WDNHVSODFH$Q06VFDQFDQEHDIXOOVFDQH[SHULPHQWRUD VHOHFWHGLRQPRQLWRULQJ6,0H[SHULPHQWVHHEHORZ MS/MS Scan $Q0606VFDQFRUUHVSRQGVWRWZRVWDJHVRIPDVVDQDO\VLVQ VFDQSRZHU,QDQ0606VFDQSDUHQWLRQVDUHIUDJPHQWHGLQWR SURGXFWLRQV$Q0606VFDQFDQEHDIXOOVFDQH[SHULPHQWRUD VHOHFWHGUHDFWLRQPRQLWRULQJ650H[SHULPHQWVHHEHORZ 1-10 Finnigan: LCQ MS Detector Hardware Manual

41 INTRODUCTION Scan Power MS n Scan $Q06 Q VFDQLQYROYHVWKUHHWRWHQVWDJHVRIPDVVDQDO\VLVQ WRQ VFDQSRZHU>+RZHYHUWKHWHUPFDQDOVREHDSSOLHGWR RQHVWDJHRIPDVVDQDO\VLVZLWKQ RUWRWZRVWDJHVRIPDVV Q VFDQFDQEHHLWKHUDIXOOVFDQ H[SHULPHQWRUDFRQVHFXWLYHUHDFWLRQPRQLWRULQJ&50 H[SHULPHQWVHHEHORZ Finnigan: LCQ MS Detector Hardware Manual 1-11

42 INTRODUCTION Scan Modes 1.4 Scan Modes <RXFDQRSHUDWHWKH/&4LQWKHIROORZLQJVFDQPRGHV )XOOVFDQ 6HOHFWHGLRQPRQLWRULQJ6,0 6HOHFWHGUHDFWLRQPRQLWRULQJ650 &RQVHFXWLYHUHDFWLRQPRQLWRULQJ&50 =RRP6FDQ Full Scan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ingle-Stage Full Scan 7KHVLQJOHVWDJHIXOOVFDQPRGHKDVRQHVWDJHRIPDVVDQDO\VLV Q VFDQSRZHU:LWKVLQJOHVWDJHIXOOVFDQPRGHWKHLRQV IRUPHGLQWKHLRQVRXUFHDUHVWRUHGLQWKHPDVVDQDO\]HU7KHQ WKHVHLRQVDUHVHTXHQWLDOO\VFDQQHGRXWRIWKHPDVVDQDO\]HUWR SURGXFHDIXOOPDVVVSHFWUXP 1-12 Finnigan: LCQ MS Detector Hardware Manual

43 INTRODUCTION Scan Modes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wo-Stage Full Scan 7KHWZRVWDJHIXOOVFDQPRGHKDVWZRVWDJHVRIPDVVDQDO\VLV Q VFDQSRZHU,QWKHILUVWVWDJHRIPDVVDQDO\VLVWKHLRQV IRUPHGLQWKHLRQVRXUFHDUHVWRUHGLQWKHPDVVDQDO\]HU7KHQ LRQVRIRQHPDVVWRFKDUJHUDWLRWKHSDUHQWLRQVDUHVHOHFWHGDQG DOORWKHULRQVDUHHMHFWHGIURPWKHPDVVDQDO\]HU7KHSDUHQWLRQV DUHH[FLWHGVRWKDWWKH\FROOLGHZLWKEDFNJURXQGJDVWKDWLV SUHVHQWLQWKHPDVVDQDO\]HU7KHFROOLVLRQVRIWKHSDUHQWLRQV FDXVHWKHPWRIUDJPHQWWRSURGXFHRQHRUPRUHSURGXFWLRQV,QWKHVHFRQGVWDJHRIPDVVDQDO\VLVWKHSURGXFWLRQVDUHVWRUHG LQWKHPDVVDQDO\]HU7KHQWKH\DUHVHTXHQWLDOO\VFDQQHGRXWRI WKHPDVVDQDO\]HUWRSURGXFHDIXOOSURGXFWLRQPDVVVSHFWUXP 7KHWZRVWDJHIXOOVFDQPRGHJLYHV\RXPRUHLQIRUPDWLRQDERXWD VDPSOHWKDQGRHV650EXWWZRVWDJHIXOOVFDQPRGHGRHVQRW \LHOGWKHVSHHGWKDWFDQEHDFKLHYHGE\650:LWKWZRVWDJHIXOO VFDQ\RXVSHQGPRUHWLPHPRQLWRULQJWKHSURGXFWLRQVWKDQ\RX GRLQ6507KXVWZRVWDJHIXOOVFDQSURYLGHVJUHDWHU LQIRUPDWLRQEXWORZHUVSHHGWKDQ650GRHV 7RXVHWKH650VFDQPRGH\RXQHHGWRNQRZZKDWUHDFWLRQ\RX DUHORRNLQJIRUEHIRUH\RXFDQSHUIRUPDQH[SHULPHQW7KXVIRU 650\RXPLJKWXVHRQHVWDJHIXOOVFDQPRGHWRGHWHUPLQHWKH SDUHQWPDVVVSHFWUXPDQGWZRVWDJHIXOOVFDQPRGHWRGHWHUPLQH WKHSURGXFWPDVVVSHFWUDIRUSDUHQWLRQVRILQWHUHVW7KHQ\RX PLJKWXVH650WRGRURXWLQHTXDQWLWDWLYHDQDO\VLVRIWKH FRPSRXQG Finnigan: LCQ MS Detector Hardware Manual 1-13

44 INTRODUCTION Scan Modes Multi-Stage Full Scan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elected Ion Monitoring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innigan: LCQ MS Detector Hardware Manual

45 INTRODUCTION Scan Modes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elected Reaction Monitoring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innigan: LCQ MS Detector Hardware Manual 1-15

46 INTRODUCTION Scan Modes SDUHQWLRQRIWKHVDPHPDVVWRFKDUJHUDWLRDVWKHVHOHFWHGSDUHQW LRQIURPWKHWDUJHWFRPSRXQG6HFRQGLWPXVWDOVRIUDJPHQWWR IRUPDSURGXFWLRQRIWKHVDPHPDVVWRFKDUJHUDWLRDVWKH VHOHFWHGSURGXFWLRQIURPWKHWDUJHWFRPSRXQG Consecutive Reaction Monitoring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innigan: LCQ MS Detector Hardware Manual

47 INTRODUCTION Scan Modes ZoomScan 7KHGHWHUPLQDWLRQRIWKHPDVVRIDQLRQIURPLWVPDVVWRFKDUJH UDWLRPD\EHFRPSOLFDWHGE\WKHIDFWWKDWWKHFKDUJHVWDWHRIWKH LRQPD\EHXQNQRZQ=RRP6FDQLVDKLJKUHVROXWLRQ06VFDQ PRGHLQZKLFK/&4SHUIRUPVDKLJKUHVROXWLRQVFDQWKDWDOORZV \RXWRGHWHUPLQHWKHFKDUJHVWDWHDQGPROHFXODUZHLJKWRIDQLRQ /&4FRQGXFWVDKLJKUHVROXWLRQVFDQRIXZLGWKDQGHYDOXDWHV WKH & &LVRWRSLFVHSDUDWLRQRIDVSHFLILHGLRQRULRQV,IWKH LVRWRSLFSHDNVDUHXDSDUWWKHLRQKDVDFKDUJHVWDWHRI±,I WKHLVRWRSLFSHDNVDUHXDSDUWWKHLRQKDVDFKDUJHVWDWHRI±,IWKHLVRWRSLFSHDNVDUHXDSDUWWKHLRQKDVDFKDUJHVWDWHRI ±HWF<RXFDQWKHQGHWHUPLQHWKHPROHFXODUZHLJKWRIWKHLRQ IURPDNQRZOHGJHRIWKHFKDUJHVWDWHDQGPDVVWRFKDUJHUDWLRRI WKHLRQ<RXPD\FRQGXFWD=RRP6FDQDQDO\VLVRIXSWRWHQLRQV E\VSHFLI\LQJWKHPDVVWRFKDUJHUDWLRVRIWKHLRQV Finnigan: LCQ MS Detector Hardware Manual 1-17

48 INTRODUCTION Scan Data Types 1.5 Scan Data Types <RXFDQDFTXLUHDQGGLVSOD\PDVVVSHFWUDOGDWDLQWHQVLW\YHUVXV PDVVWRFKDUJHUDWLRZLWKWKH/&4LQRQHRIWZRVFDQGDWDW\SHV 3URILOHVFDQ &HQWURLGVFDQ Profile Scan,QWKHSURILOHVFDQGDWDW\SH\RXFDQVHHWKHVKDSHRIWKHSHDNV LQWKHPDVVVSHFWUXP(DFKDWRPLFPDVVXQLWLVGLYLGHGLQWR DSSUR[LPDWHO\VDPSOLQJLQWHUYDOV7KHLQWHQVLW\RIWKHLRQ FXUUHQWLVGHWHUPLQHGDWHDFKRIWKHVDPSOLQJLQWHUYDOV7KH LQWHQVLW\DWHDFKVDPSOLQJLQWHUYDOLVGLVSOD\HGZLWKWKH LQWHQVLWLHVFRQQHFWHGE\DFRQWLQXRXVOLQH,QJHQHUDOWKHSURILOH VFDQGDWDW\SHLVXVHGZKHQ\RXWXQHDQGFDOLEUDWHWKH06 GHWHFWRUVRWKDW\RXFDQHDVLO\VHHDQGPHDVXUHPDVVUHVROXWLRQ Centroid Scan,QWKHFHQWURLGVFDQGDWDW\SHWKHPDVVVSHFWUXPLVGLVSOD\HG DVDEDUJUDSK,QWKLVVFDQGDWDW\SHWKHLQWHQVLWLHVRIHDFKVHW RIVDPSOLQJLQWHUYDOVDUHVXPPHG7KLVVXPLVGLVSOD\HG YHUVXVWKHLQWHJUDOFHQWHURIPDVVRIWKHVDPSOLQJLQWHUYDOV,Q JHQHUDOWKHFHQWURLGVFDQGDWDW\SHLVXVHGIRUGDWDDFTXLVLWLRQ EHFDXVHWKHVFDQVSHHGLVIDVWHU'DWDSURFHVVLQJLVDOVRPXFK IDVWHUIRUFHQWURLGGDWD 1-18 Finnigan: LCQ MS Detector Hardware Manual

49 FUNCTIONAL DESCRIPTION Contents Contents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innigan: LCQ MS Detector Hardware Manual 2-i

50 FUNCTIONAL DESCRIPTION Contents 5):DYHIRUP9ROWDJH*HQHUDWLRQ(OHFWURQLF$VVHPEOLHV,RQ'HWHFWLRQ6\VWHP(OHFWURQLF$VVHPEOLHV (PEHGGHG&RPSXWHU(OHFWURQLF$VVHPEOLHV 'DWD6\VWHP &RPSXWHU+DUGZDUH &RPSXWHU6RIWZDUH 'DWD6\VWHP06'HWHFWRU,QWHUIDFH 'DWD6\VWHP/RFDO$UHD1HWZRUN,QWHUIDFH 3ULQWHU List of Illustrations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ii Finnigan: LCQ MS Detector Hardware Manual

51 FUNCTIONAL DESCRIPTION 2. FUNCTIONAL DESCRIPTION 7KLVFKDSWHUGHVFULEHVWKHSULQFLSDOFRPSRQHQWVRIWKH/&4 V\VWHPDQGWKHLUUHVSHFWLYHIXQFWLRQV7KHSULQFLSDOFRPSRQHQWV RIWKH/&4V\VWHPDUHDVIROORZV $XWRVDPSOHURSWLRQDO /LTXLGFKURPDWRJUDSKRSWLRQDO 6\ULQJHSXPS 'LYHUWLQMHFWYDOYH 06GHWHFWRU 'DWDV\VWHP $IXQFWLRQDOEORFNGLDJUDPRIWKH/&4V\VWHPLVVKRZQLQ)LJXUH $VDPSOHWUDQVIHUOLQHFRQQHFWVWKH/&WRWKH06GHWHFWRU 7KHDXWRVDPSOHUDQG/&DUHXVXDOO\LQVWDOOHGRQWKHOHIWRIWKH 06GHWHFWRU7KHV\ULQJHSXPSDQGGLYHUWLQMHFWYDOYHDUH LQWHJUDWHGLQWRWKH06GHWHFWRUFDELQHW 6DPSOHVDUHLQMHFWHGHLWKHUPDQXDOO\RUE\DQDXWRVDPSOHULQWR WKH/&LQOHW7KH/&WKHQVHSDUDWHVWKHVDPSOHPROHFXOHVE\ OLTXLGFKURPDWRJUDSK\7KHVHSDUDWHGFRQVWLWXHQWVIURPWKH/& IORZWKURXJKWKHWUDQVIHUOLQHDQGDUHLQWURGXFHGLQWRWKH DWPRVSKHULFSUHVVXUHLRQL]DWLRQ$3,VRXUFH<RXFDQDOVRLQMHFW LQIXVHVDPSOHVLQWRWKH$3,VRXUFHZLWKWKHV\ULQJHSXPSRUWKH GLYHUWLQMHFWYDOYH 8SRQHQWHULQJWKH$3,VRXUFHVDPSOHPROHFXOHVDUHLRQL]HGE\ HOHFWURVSUD\LRQL]DWLRQ(6,RUDWPRVSKHULFSUHVVXUHFKHPLFDO LRQL]DWLRQ$3&,7KHLRQRSWLFVIRFXVDQGDFFHOHUDWHWKH UHVXOWLQJVDPSOHLRQVLQWRWKHPDVVDQDO\]HUZKHUHWKH\DUH DQDO\]HGDFFRUGLQJWRWKHLUPDVVWRFKDUJHUDWLRV7KHVDPSOH LRQVDUHWKHQGHWHFWHGE\DQLRQGHWHFWLRQV\VWHPWKDWSURGXFHVD Finnigan: LCQ MS Detector Hardware Manual 2-1

52 FUNCTIONAL DESCRIPTION VLJQDOSURSRUWLRQDOWRWKHQXPEHURILRQVGHWHFWHG7KHLRQ FXUUHQWVLJQDOIURPWKHLRQGHWHFWLRQV\VWHPLVUHFHLYHGDQG DPSOLILHGE\WKHV\VWHPHOHFWURQLFVDQGLVWKHQSDVVHGRQWRWKH GDWDV\VWHPIRUIXUWKHUSURFHVVLQJVWRUDJHDQGGLVSOD\7KHGDWD V\VWHPSURYLGHVWKHSULPDU\/&4XVHULQWHUIDFH Figure 2-1. Functional block diagram of the LCQ system. The broad, single-headed arrows represent the flow of sample molecules through the instrument. The narrow, double-headed arrows represent electrical connections. 2-2 Finnigan: LCQ MS Detector Hardware Manual

53 FUNCTIONAL DESCRIPTION Autosampler 2.1 Autosampler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innigan: LCQ MS Detector Hardware Manual 2-3

54 FUNCTIONAL DESCRIPTION Liquid Chromatograph 2.2 Liquid Chromatograph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innigan: LCQ MS Detector Hardware Manual

55 FUNCTIONAL DESCRIPTION Syringe Pump 2.3 Syringe Pump 7KH/&4LQFOXGHVDQHOHFWURQLFDOO\FRQWUROOHGLQWHJUDWHGGXDO V\ULQJHSXPS7KHV\ULQJHSXPSGHOLYHUVVDPSOHVROXWLRQDQGRU VKHDWKOLTXLGIURPWKHV\ULQJHVLQWRWKH$3,VRXUFH6HH )LJXUH:KHQWKHV\ULQJHSXPSLVRSHUDWLQJDPRWRUGULYHVD SXVKHUEORFNWKDWGHSUHVVHVWKHSOXQJHURIWKHV\ULQJHDWD FRQWUROOHGUDWH/LTXLGIORZVRXWRIWKHV\ULQJHQHHGOHDQGLQWRWKH VDPSOHWUDQVIHUOLQHRUVKHDWKOLTXLGOLQHDVWKHSOXQJHULV GHSUHVVHG7KHV\ULQJHLVKHOGLQSODFHE\DV\ULQJHKROGHU 5HIHUWRWKHWRSLF6HWWLQJ8SWKH,QOHW6\ULQJH3XPSLQWKH /&42SHUDWRUª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igure 2-2. Syringe pump Finnigan: LCQ MS Detector Hardware Manual 2-5

56 FUNCTIONAL DESCRIPTION Divert/Inject Valve 2.4 Divert/Inject Valve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igure 2-3. Divert/inject valve 2-6 Finnigan: LCQ MS Detector Hardware Manual

57 FUNCTIONAL DESCRIPTION MS Detector 2.5 MS Detector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innigan: LCQ MS Detector Hardware Manual 2-7

58 FUNCTIONAL DESCRIPTION MS Detector Controls and Indicators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igure 2-4. Front panel LEDs of the MS detector 2-8 Finnigan: LCQ MS Detector Hardware Manual

59 FUNCTIONAL DESCRIPTION MS Detector 7ZRDGGLWLRQDO/('VDQGDSXVKEXWWRQVZLWFKDUHORFDWHGRQWKH IURQWSDQHODERYHWKHGLYHUWLQMHFWYDOYH6HH)LJXUH:KHQ WKHGLYHUWLQMHFWYDOYHLVVHWXSIRUORRSLQMHFWLRQVWKHGLYHUWLQMHFW YDOYHEXWWRQWRJJOHVWKHYDOYHEHWZHHQORDGDQGLQMHFWPRGHVDQG WKHODEHOV/RDGDQG,QMHFWDSSO\:KHQWKHGLYHUWLQMHFWYDOYHLV VHWXSIRUGLYHUWYDOYHRSHUDWLRQWKHGLYHUWLQMHFWYDOYHEXWWRQ WRJJOHVWKH/&IORZEHWZHHQWKH06GHWHFWRUDQGWKHZDVWH FRQWDLQHUDQGWKHODEHOV'HWHFWRUDQG:DVWHDSSO\ Figure 2-5. Divert/inject valve button and LEDs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innigan: LCQ MS Detector Hardware Manual 2-9

60 FUNCTIONAL DESCRIPTION MS Detector RESET BUTTON ETHERNET CONNECTO ELECTRONICS SERVICE SWITCH LINE VOLTAGE SWITCH LINE POWER ROTARY-VANE PUMP POWER OUTLET MAIN POWER CIRCUIT BREAKER SWITCH Figure 2-6. Power panel 7KHUHVHWEXWWRQODEHOHG5HVHWLVDOVRORFDWHGRQWKHSRZHU SDQHO:KHQ\RXSUHVVWKHUHVHWEXWWRQ/&4VRIWZDUHLVUHORDGHG IURPWKHGDWDV\VWHP5HIHUWRWKHWRSLF5HVHWWLQJWKH06 'HWHFWRULQWKH6\VWHP6KXWGRZQ6WDUWXSDQG5HVHW FKDSWHUIRULQIRUPDWLRQRQUHVHWWLQJWKH06GHWHFWRU 2-10 Finnigan: LCQ MS Detector Hardware Manual

61 FUNCTIONAL DESCRIPTION MS Detector API Source 7KHDWPRVSKHULFSUHVVXUHLRQL]DWLRQ$3,VRXUFHIRUPVJDV SKDVHVDPSOHLRQVIURPVDPSOHPROHFXOHVWKDWDUHFRQWDLQHGLQ VROXWLRQ7KH$3,VRXUFHDOVRVHUYHVDVWKHVDPSOHLQWHUIDFH EHWZHHQWKH/&DQGWKH06GHWHFWRU<RXFDQRSHUDWHWKH$3, VRXUFHLQHLWKHUWKHHOHFWURVSUD\LRQL]DWLRQ(6,RUDWPRVSKHULF SUHVVXUHFKHPLFDOLRQL]DWLRQ$3&,PRGH 7KH$3,VRXUFHFRQVLVWVRIWZRDVVHPEOLHV $3,SUREHDVVHPEO\(6,RU$3&, $3,VWDFN API Probe Assembly 7KH$3,SUREHDVVHPEO\LVWKHSRUWLRQRIWKH$3,VRXUFHWKDWLV H[WHUQDOWRWKHYDFXXPPDQLIROG<RXQHHGWRVZLWFKSUREH DVVHPEOLHVZKHQ\RXFKDQJHLRQL]DWLRQPRGHV7ZR$3,SUREH DVVHPEOLHVDUHDYDLODEOHZLWKWKH/&4 (6,SUREHDVVHPEO\ $3&,SUREHDVVHPEO\ ESI Probe Assembly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innigan: LCQ MS Detector Hardware Manual 2-11

62 FUNCTIONAL DESCRIPTION MS Detector FLANGE RETAINER BOLT (2 ) ESI FLANGE ESI PROBE GROUNDED FITTING SHEATH GAS INLET PROBE RETAINER BOLT SPRAY SHIELD API STACK CABLE CONNECTOR AUXILIARY GAS INLET SAMPLE INLET SHEATH LIQUID INLET PROBE SLIDE ADAPTER ESI NEEDLE HIGH VOLTAGE CONNECTOR Figure 2-7. ESI probe assembly 7KH(6,SUREHSURGXFHVFKDUJHGDHURVROGURSOHWVWKDWFRQWDLQ VDPSOHLRQV7KH(6,SUREHDFFRPPRGDWHVOLTXLGIORZVRI µ/plq WRP/PLQ ZLWKRXWVSOLWWLQJ 7KH(6,SUREHLQFOXGHVWKH(6,VDPSOHWXEHQHHGOHQR]]OHDQG PDQLIROG6DPSOHDQGVROYHQWHQWHUWKH(6,SUREHWKURXJKWKH VDPSOHWXEH7KHVDPSOHWXEHLVDVKRUWVHFWLRQRIPP,' IXVHGVLOLFDWXELQJWKDWH[WHQGVIURPDILWWLQJVHFXUHGWRWKH JURXQGHGILWWLQJKROGHUWKURXJKWKHVDPSOHLQOHWDQGLQWRWKH(6, QHHGOHWRZLWKLQPPIURPWKHHQGRIWKH(6,QHHGOH7KH(6, QHHGOHWRZKLFKDODUJHQHJDWLYHRUSRVLWLYHYROWDJHLVDSSOLHG W\SLFDOO\±WR±N9VSUD\VWKHVDPSOHVROXWLRQLQWRDILQH PLVWRIFKDUJHGGURSOHWV7KH(6,QR]]OHGLUHFWVWKHIORZRI VKHDWKJDVDQGDX[LOLDU\JDVDWWKHGURSOHWV7KH(6,PDQLIROG 2-12 Finnigan: LCQ MS Detector Hardware Manual

63 FUNCTIONAL DESCRIPTION MS Detector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µ/PLQ DQGXQLWVIRUVDPSOHIORZ UDWHVRIP/PLQ 7\SLFDOVKHDWKJDVIORZUDWHVIRU$3&,DUH XQLWVIRUVDPSOHIORZUDWHVRIµ/PLQ XQLWVIRUVDPSOH IORZUDWHVRIP/PLQ DQGXQLWVIRUVDPSOHIORZUDWHVRI P/PLQ :KHQ\RXWXQHWKH/&4\RXVKRXOGDGMXVWWKH VKHDWKJDVIORZUDWHXQWLOWKHLRQVLJQDOLVVWDEOH Figure 2-8. Cross sectional view of the ESI probe assembly Finnigan: LCQ MS Detector Hardware Manual 2-13

64 FUNCTIONAL DESCRIPTION MS Detector 7KHDX[LOLDU\JDVLVWKHRXWHUFRD[LDOQLWURJHQJDVWKDWDVVLVWV WKHVKHDWKJDVLQWKHQHEXOL]DWLRQDQGHYDSRUDWLRQRIVDPSOH VROXWLRQV7KHDX[LOLDU\JDVDOVRKHOSVORZHUWKHKXPLGLW\LQWKH LRQVRXUFH7\SLFDODX[LOLDU\JDVIORZUDWHVIRU(6,DQG$3&,DUH WRXQLWV$X[LOLDU\JDVLVXVXDOO\QRWQHHGHGIRUVDPSOHIORZ UDWHVEHORZµ/PLQ 7KHVKHDWKOLTXLGLVDVROYHQWXVHGWRVWDELOL]HDQGHQKDQFHWKH (6,SURFHVVIRUVRPHVROXWLRQFKHPLVWULHVIRUH[DPSOHKLJK DTXHRXVFRQWHQWWKDWGRQRWUHDGLO\IRUPDQHOHFWURVSUD\6KHDWK OLTXLGLVLQMHFWHGE\WKHV\ULQJHSXPSDQGH[LWVWKHQR]]OH FRD[LDOO\WRWKHVDPSOHWXEH APCI Probe Assembly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µ/plq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innigan: LCQ MS Detector Hardware Manual

65 FUNCTIONAL DESCRIPTION MS Detector APCI FLANGE VAPORIZER HEATER CABLE CONNECTOR FLANGE RETAINE R BOLT APCI AUXILIARY GAS INLET SAMPLE INLET (RED) PROBE SLIDE ADAPTER CORONA NEEDLE HIGH VOLTAGE PROBE RETAINER BOLT SHEATH GAS INLET (BLUE) Figure 2-9. APCI probe assembly 7\SLFDOYDSRUL]HUWHPSHUDWXUHVDUHWR &IRUIORZUDWHVRI µ/plq WR &IRUP/PLQ QRUPDO$3&,IORZ UDWHDQGWR &IRUP/PLQ 7KHVDPSOHYDSRULV VZHSWWRZDUGWKHFRURQDGLVFKDUJHQHHGOHE\WKHIORZRIWKH VKHDWKDQGDX[LOLDU\JDVVHV 7KHFRURQDGLVFKDUJHQHHGOHDVVHPEO\LVPRXQWHGRQWKH$3&, IODQJH7KHDVVHPEO\SRVLWLRQVWKHWLSRIWKHFRURQDGLVFKDUJH QHHGOHQHDUWKHYDSRUL]HU$KLJKSRWHQWLDOW\SLFDOO\±WR±N9 LVDSSOLHGWRWKHFRURQDGLVFKDUJHQHHGOHWRSURGXFHDFRURQD GLVFKDUJHFXUUHQWRIXSWRµ$$W\SLFDOYDOXHRIWKHFRURQD GLVFKDUJHFXUUHQWLVµ$7KHFRURQDGLVFKDUJHIURPWKHQHHGOH SURGXFHVDUHDJHQWLRQSODVPDSULPDULO\IURPWKHVROYHQWYDSRU Finnigan: LCQ MS Detector Hardware Manual 2-15

66 FUNCTIONAL DESCRIPTION MS Detector Figure Cross sectional view of the APCI probe assembly 7KHVDPSOHYDSRULVLRQL]HGE\LRQPROHFXOHUHDFWLRQVZLWKWKH UHDJHQWLRQVLQWKHSODVPD $3&,UHTXLUHVDFRQVWDQWVRXUFHRIHOHFWURQVIRUWKHLRQL]DWLRQ SURFHVV7KXVWKHFRURQDGLVFKDUJHFXUUHQWLVVHWDQGUHJXODWHG 7KHSRWHQWLDODSSOLHGWRWKHFRURQDGLVFKDUJHQHHGOHYDULHVDV QHHGHGWRSURYLGHWKHUHTXLUHGFXUUHQW API Stack 7KH$3,VWDFNFRQVLVWVRIWKHFRPSRQHQWVRIWKH$3,VRXUFHWKDW DUHKHOGXQGHUYDFXXPH[FHSWIRUWKHDWPRVSKHULFSUHVVXUHVLGH RIWKHVSUD\VKLHOG7KH$3,VWDFNLQFOXGHVWKHVSUD\VKLHOG KHDWHGFDSLOODU\WXEHOHQVVNLPPHUKHDWHGFDSLOODU\PRXQWDQG WXEHOHQVDQGVNLPPHUPRXQW6HH)LJXUH7KHVDPH$3, VWDFNLVXVHGIRUERWK(6,DQG$3&,LRQL]DWLRQPRGHV 2-16 Finnigan: LCQ MS Detector Hardware Manual

67 FUNCTIONAL DESCRIPTION MS Detector Figure Cross sectional view of the API stack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ƒ&7\SLFDOWHPSHUDWXUHVRIWKHKHDWHG Finnigan: LCQ MS Detector Hardware Manual 2-17

68 FUNCTIONAL DESCRIPTION MS Detector FDSLOODU\DUHƒ&IRU(6,DQGƒ&IRU$3&,7KHKHDWHG FDSLOODU\SDVVHVWKURXJKDKROHLQWKHFHQWHURIWKHVSUD\VKLHOG,RQVDUHGUDZQLQWRWKHKHDWHGFDSLOODU\LQWKHDWPRVSKHULF SUHVVXUHUHJLRQDQGWUDQVSRUWHGWRWKHFDSLOODU\VNLPPHUUHJLRQ RIWKHYDFXXPPDQLIROGE\DGHFUHDVLQJSUHVVXUHJUDGLHQW$ SRWHQWLDORIW\SLFDOO\WR±9SRVLWLYHIRUSRVLWLYHLRQVDQG QHJDWLYHIRUQHJDWLYHLRQVDVVLVWVLQUHSHOOLQJLRQVIURPWKH KHDWHGFDSLOODU\WRWKHVNLPPHU,RQVIURPWKHKHDWHGFDSLOODU\HQWHUWKHWXEHOHQV7KHWXEHOHQV DOVRFDOOHGWKHWXEHJDWHKDVDPDVVGHSHQGHQWSRWHQWLDODSSOLHG WRLWWRIRFXVWKHLRQVWRZDUGVWKHRSHQLQJRIWKHVNLPPHU$Q DGGLWLRQDOSRWHQWLDORIEHWZHHQDQG±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innigan: LCQ MS Detector Hardware Manual

69 FUNCTIONAL DESCRIPTION MS Detector H[LWHQGRIWKHKHDWHGFDSLOODU\LVDWWKHSURSHUGLVWDQFHIURPWKH RSHQLQJLQWKHVNLPPHU Ion Optics,RQVHQWHUWKHLRQRSWLFVDIWHUSDVVLQJWKURXJKWKHVNLPPHU7KH LRQRSWLFVWUDQVPLWLRQVIURPWKH$3,VRXUFHWRWKHPDVV DQDO\]HU7KHLRQRSWLFVFRQVLVWRIWZRRFWDSROHVDQGDQ LQWHURFWDSROHOHQV6HH)LJXUHDQG)LJXUH(DFK RFWDSROHLVDQRFWDJRQDODUUD\RIF\OLQGULFDOURGVWKDWDFWVDVDQ LRQWUDQVPLVVLRQGHYLFH$Q5)YROWDJH0+]9SHDNWR SHDNDQGGFRIIVHWYROWDJHW\SLFDOO\WR9WKDWDUHDSSOLHG WRWKHURGVJLYHULVHWRDQHOHFWULFILHOGWKDWJXLGHVWKHLRQVDORQJ WKHD[LVRIWKHRFWDSROH'XULQJLRQWUDQVPLVVLRQWKHRIIVHW YROWDJHLVQHJDWLYHIRUSRVLWLYHLRQVDQGSRVLWLYHIRUQHJDWLYHLRQV 7KHRFWDSROH5)YROWDJHLVWXUQHGRIIGXULQJPDVVDQDO\VLV 7KHWZRRFWDSROHVDUHVHSDUDWHGE\WKHLQWHURFWDSROHOHQV7KH LQWHURFWDSROHOHQVDVVLVWVLQWKHIRFXVLQJDQGJDWLQJRILRQV7KH LQWHURFWDSROHOHQVDOVRVHUYHVDVDEDIIOHEHWZHHQWKHILUVWRFWDSROH UHJLRQDQGWKHDQDO\]HUUHJLRQRIWKHYDFXXPPDQLIROG7KH/&4 WXQHSURFHGXUHRSWLPL]HVWKHSRWHQWLDOVWKDWDUHDSSOLHGWRWKH RFWDSROHVDQGLQWHURFWDSROHOHQVWRPD[LPL]HWKHLRQFXUUHQWWRWKH PDVVDQDO\]HU'XULQJLRQWUDQVPLVVLRQDSRWHQWLDORIW\SLFDOO\ EHWZHHQDQG9LVDSSOLHGWRWKHLQWHURFWDSROHOHQV7KH SRWHQWLDOLVQHJDWLYHIRUSRVLWLYHLRQVDQGSRVLWLYHIRUQHJDWLYH LRQV'XULQJJDWLQJWKHSRWHQWLDOLV9IRUSRVLWLYHLRQVDQG 9IRUQHJDWLYHLRQV Finnigan: LCQ MS Detector Hardware Manual 2-19

70 FUNCTIONAL DESCRIPTION MS Detector Figure Cross sectional view of the ion optics Mass Analyzer 7KHTXDGUXSROHLRQWUDSPDVVDQDO\]HULVWKHVLWHRIPDVV DQDO\VLVWKDWLVLRQVWRUDJHLRQLVRODWLRQFROOLVLRQLQGXFHG GLVVRFLDWLRQDQGLRQVFDQRXW7KLVVHFWLRQGHVFULEHVWKH FRPSRQHQWVRIWKHPDVVDQDO\]HUWKHYROWDJHVDSSOLHGWRWKHPDVV DQDO\]HUHOHFWURGHVWKHSUHVHQFHRIKHOLXPGDPSLQJJDVLQWKH PDVVDQDO\]HUFDYLW\DQGWKHRSHUDWLRQRIWKHPDVVDQDO\]HU GXULQJPDVVDQDO\VLV Components of the Mass Analyzer 7KHPDVVDQDO\]HULVPRXQWHGRQWKHDQDO\]HUPRXQWRSSRVLWHWKH VHFRQGRFWDSROH7KHPDVVDQDO\]HULVVKRZQLQFURVVVHFWLRQLQ )LJXUHDQGLQDSKRWRJUDSKLQ)LJXUH 7KHPDVVDQDO\]HULQFOXGHVWKUHHVWDLQOHVVVWHHOHOHFWURGHVWKH HQWUDQFHHQGFDSHOHFWURGHWKHH[LWHQGFDSHOHFWURGHDQGWKHULQJ HOHFWURGH7KHLQQHUVXUIDFHVRIHOHFWURGHVDUHK\SHUEROLF 7RJHWKHUWKH\IRUPDFDYLW\LQZKLFKPDVVDQDO\VLVRFFXUV 2-20 Finnigan: LCQ MS Detector Hardware Manual

71 FUNCTIONAL DESCRIPTION MS Detector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innigan: LCQ MS Detector Hardware Manual 2-21

72 FUNCTIONAL DESCRIPTION MS Detector Figure Cross sectional view of the mass analyzer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arious ac voltages are applied to the ring and endcap electrodes to trap, fragment, and eject ions according to their mass-to-charge ratios. These ac voltages, referred to as the ring electrode RF voltage, waveform voltage, resonance excitation RF voltage, and resonance ejection RF voltage, are discussed below Ring Electrode RF Voltage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innigan: LCQ MS Detector Hardware Manual

73 FUNCTIONAL DESCRIPTION MS Detector :KHQWKHDPSOLWXGHRIWKHULQJHOHFWURGH5)YROWDJHLVORZDOO LRQVDERYHDPLQLPXPPDVVWRFKDUJHUDWLRDUHWUDSSHG7KLV5) YROWDJHLVUHIHUUHGWRDVWKHVWRUDJHYROWDJHDQGWKHPLQLPXP PDVVWRFKDUJHUDWLRLVXVXDOO\FKRVHQWREHJUHDWHUWKDQWKH PDVVWRFKDUJHUDWLRVDVVRFLDWHGZLWKDLUZDWHUDQGVROYHQWLRQV During ion scan out, the ring electrode RF voltage is ramped at a constant rate corresponding to approximately 5,500 u s. $VWKH ULQJHOHFWURGH5)YROWDJHLQFUHDVHVLRQVRILQFUHDVLQJPDVVWR FKDUJHUDWLREHFRPHVXFFHVVLYHO\XQVWDEOHLQWKHD[LDOGLUHFWLRQ DQGDUHHMHFWHGIURPWKHPDVVDQDO\]HUThe voltage at which an ion is ejected from the mass analyzer is defined as its resonance voltage. The ejection of ions of each mass-to-charge ratio occurs over a very short time. Many of these ions are detected by the ion detection system Ion Injection Waveform Voltage, Ion Isolation Waveform Voltage, Resonance Excitation RF Voltage, and Resonance Ejection RF Voltage Applied to the Endcap Electrodes 7KHLRQLQMHFWLRQZDYHIRUPYROWDJHLRQLVRODWLRQZDYHIRUP YROWDJHUHVRQDQFHH[FLWDWLRQ5)YROWDJHDQGUHVRQDQFHHMHFWLRQ 5)YROWDJHDUHDFYROWDJHVWKDWDUHDSSOLHGWRWKHHQGFDS HOHFWURGHVWRVWLPXODWHPRWLRQRIWKHLRQVLQWKHD[LDOGLUHFWLRQ 7KHYROWDJHVDSSOLHGWRWKHHQGFDSHOHFWURGHVDUHHTXDOLQ DPSOLWXGHEXWDUH R RXWRISKDVHWRRQHDQRWKHUWhen the RF frequency applied to the endcaps equals the resonance frequency of a trapped ion, which depends on its mass, the ion gains kinetic energy. If the magnitude of the applied voltage is large enough, the ion is ejected from the mass analyzer in the axial direction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innigan: LCQ MS Detector Hardware Manual 2-23

74 FUNCTIONAL DESCRIPTION MS Detector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uring the collision induced dissociation step of 650&50DQG 06 Q IXOOVFDQZLWKQ!applications, the resonance excitation RF voltage is applied to the endcap electrodes to fragment parent ions into product ions. The resonance excitation RF voltage is not strong enough to eject an ion from the mass analyzer. However, ion motion in the axial direction is enhanced and the ion gains kinetic energy. After many collisions with the helium damping gas, which is present in the mass analyzer, the ion gains enough internal energy to cause it to dissociate into product ions. The product ions are then mass analyzed. During ion scan out, the resonance ejection RF voltage facilitates the ejection of ions from the mass analyzer and thus improves mass resolution. The resonance ejection RF voltage is applied at fixed frequency and increasing amplitude during the ramp of the ring electrode RF voltage. Only when an ion is about to be ejected from the mass analyzer cavity by the ring electrode RF voltage is it in resonance with the resonance ejection RF voltage. When an ion comes into resonance, it moves farther away from the center of the mass analyzer, where the field generated by the ring electrode RF voltage is zero (and space-charge effects are strong), into a region where the field produced by the ring electrode RF voltage is strong (and space-charge effects are small). As a result, the ejection of the ion is facilitated, and mass resolution is significantly improved Helium Damping Gas in the Mass Analyzer Cavity The mass analyzer cavity contains helium that is used as a damping gas and a collision activation partner. The helium damping gas enters the mass analyzer cavity through a nipple on the exit endcap electrode. The flow of gas (1 ml min ) into the mass analyzer cavity 2-24 Finnigan: LCQ MS Detector Hardware Manual

75 FUNCTIONAL DESCRIPTION MS Detector is regulated by a pressure regulator and a capillary restrictor. The flow of gas out of the mass analyzer cavity (and into the turbomolecular pump) is restricted by the holes in the endcap electrodes. The flows into and out of the cavity are matched so that the partial pressure of helium in the mass analyzer cavity is maintained at approximately 0.1 Pa (10 Torr). The collisions of the ions entering the mass analyzer with the helium slow the ions so that they can be trapped by the RF field in the mass analyzer. The presence of helium in the mass analyzer cavity significantly enhances sensitivity and mass spectral resolution. Before their ejection from the mass analyzer cavity, sample ions collide with helium atoms. These collisions reduce the kinetic energy of the ions, thereby damping the amplitude of their oscillations. As a result, the ions are focused into the center of the cavity rather than being allowed to spread throughout the cavity. Helium in the mass analyzer cavity also serves as a collision activation partner. During the collision induced dissociation step of an SRM, &50RU06 Q IXOOVFDQZLWKQ!analysis, the resonance excitation RF voltage applied to the endcap electrodes drives parent ions into the helium atoms. After gaining sufficient internal energy from the resulting collisions, the parent ion dissociates into one or more product ions Summary of Mass Analyzer Operation The processes that occur in the mass analyzer can be broken down into four steps:,rqvwrudjh,rqlvrodwlrq>6,0650&50dqg06 Q IXOOVFDQZLWKQ! RQO\@ &ROOLVLRQLQGXFHGGLVVRFLDWLRQ>650&50DQG06 Q IXOOVFDQ ZLWKQ!RQO\@,RQVFDQRXWWKHLRQGHWHFWLRQVWHS For 650&50DQG06 Q IXOOVFDQZLWKQ!DSSOLFDWLRQVWhe ion isolation and collision induced dissociation steps are performed n-1 times, where n is the scan power. Finnigan: LCQ MS Detector Hardware Manual 2-25

76 FUNCTIONAL DESCRIPTION MS Detector Before ion storage, the following conditions are established: +HOLXPLVSUHVHQWLQWKHPDVVDQDO\]HUFDYLW\DWDSDUWLDO SUHVVXUHRIDERXW3D 7RUU 5LQJHOHFWURGH5)YROWDJHLVVHWWRWKHVWRUDJHYROWDJH,RQLQMHFWLRQZDYHIRUPYROWDJHRQWKHHQGFDSHOHFWURGHVLVRQ LI\RXKDYHVHOHFWHGLW,RQLVRODWLRQZDYHIRUPYROWDJHUHVRQDQFHH[FLWDWLRQ5) YROWDJHDQGUHVRQDQFHHMHFWLRQ5)YROWDJHRQWKHHQGFDS HOHFWURGHVDUHRII With these conditions achieved, sample ions formed in the API source are trapped in the mass analyzer if the following conditions are met: 7KHLRQVKDYHPDVVWRFKDUJHUDWLRVJUHDWHUWKDQWKH PLQLPXPVWRUDJHPDVVWRFKDUJHUDWLR 7KHLRQVDUHQRWHMHFWHGE\WKHLRQLQMHFWLRQZDYHIRUPYROWDJH $IWHUDQRSWLPXPQXPEHURILRQVKDYHEHHQWUDSSHGWKHLRQ LQMHFWLRQZDYHIRUPYROWDJHLVWXUQHGRII7KHULQJHOHFWURGH5) VWRUDJHYROWDJHUHPDLQVRQ Next, for SIM, SRM, CRM, and 06 Q IXOOVFDQZLWKQ!analyses, the ion isolation waveform voltage is applied to the endcap electrodes,lqfrpelqdwlrqzlwkdudpsriwkhulqjhohfwurgh5) YROWDJHWRDQHZVWRUDJHYROWDJHWRHMHFWDOOLRQVH[FHSWWKRVHRI WKHVHOHFWHGPDVVWRFKDUJHUDWLR Then, for SRM, CRM, and 06 Q IXOOVFDQZLWKQ!analyses, the resonance excitation RF voltage is applied to the endcap electrodes to cause collision induced dissociation. Product ions with mass-tocharge ratio greater than the minimum storage mass-to-charge ratio are stored. (The minimum storage mass during collision induced dissociation is typically set to one quarter of the parent ion mass-tocharge ratio.) For SRM, CRM, and 06 Q IXOOVFDQZLWKQ!DSSOLFDWLRQVWhe ion isolation and collision induced dissociation steps are performed n-1 times, where n is the scan power. Finally, the sample ions or product ions are scanned out: The ring electrode RF voltage is ramped from low voltage to high voltage, and simultaneously the resonance ejection RF voltage is applied to the endcap electrodes to facilitate ejection. As the ring electrode RF 2-26 Finnigan: LCQ MS Detector Hardware Manual

77 FUNCTIONAL DESCRIPTION MS Detector voltage is increased, ions of greater and greater mass-to-charge ratios become unstable and are ejected from the mass analyzer. Many of these ions are focused toward the ion detection system where they are detected. Finnigan: LCQ MS Detector Hardware Manual 2-27

78 FUNCTIONAL DESCRIPTION MS Detector Ion Detection System The LCQ is equipped with a high sensitivity, off-axis ion detection system that produces a high signal-to-noise ratio and allows for voltage polarity switching between positive ion and negative ion modes of operation. The ion detection system includes a 15-kV conversion dynode and a channel electron multiplier. The ion detection system is located at the rear of the vacuum manifold behind the mass analyzer. See )LJXUH (cross sectional view), )LJXUH (conversion dynode), and )LJXUH (electron multiplier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he electron multiplier is mounted on the top cover plate of the vacuum manifold next to the mass analyzer. See )LJXUH and )LJXUH. The electron multiplier includes a cathode and an anode. The cathode of the electron multiplier is a lead-oxide, funnellike resistor. A potential of up to -2.5 kv is applied to the cathode by the high voltage ring. The exit end of the cathode (at the anode) is near ground potential. The cathode is held in place by the high voltage ring, two support plates, the electron multiplier support, and the electron multiplier shield. A spring washer applies a force to the cathode to hold it in contact with the electron multiplier shield. The electron multiplier support is attached to the top cover plate of the vacuum manifold by two screws. The anode of the electron multiplier is a small cup located at the exit end of the cathode. The anode collects the electrons produced by the cathode. The anode screws into the anode feedthrough in the top cover plate Finnigan: LCQ MS Detector Hardware Manual

79 FUNCTIONAL DESCRIPTION MS Detector Figure Cross sectional view of the ion detection system, showing the electron multiplier and the conversion dynode Finnigan: LCQ MS Detector Hardware Manual 2-29

80 FUNCTIONAL DESCRIPTION MS Detector Secondary particles from the conversion dynode strike the inner walls of the electron multiplier cathode with sufficient energy to eject electrons. The ejected electrons are accelerated farther into the cathode, drawn by the increasingly positive potential gradient. Due to the funnel shape of the cathode, the ejected electrons do not travel far before they again strike the inner surface of the cathode, thereby causing the emission of more electrons. Thus, a cascade of electrons is created that finally results in a measurable current at the end of the cathode where the electrons are collected by the anode. The current collected by the anode is proportional to the number of secondary particles striking the cathode. Typically, the electron multiplier is set to a gain of about 3 10 (i.e., for each ion or electron that enters, 3 10 electrons exit). The current that leaves the electron multiplier via the anode is converted to a voltage by the electrometer circuit and recorded by the data system. Refer to the topic Ion Detection System Electronic Assemblies on page The ion detection system of the LCQ increases signal and decreases noise. The high voltage applied to the conversion dynode results in a high conversion efficiency and increased signal. That is, for each ion striking the conversion dynode, many secondary particles are produced. The increase in conversion efficiency is more pronounced for more massive ions than for less massive ions. Because of the off-axis orientation of the ion detection system relative to the mass analyzer, neutral molecules from the mass analyzer tend not to strike the conversion dynode or electron multiplier. As a result, the noise from neutral molecules is reduced Finnigan: LCQ MS Detector Hardware Manual

81 FUNCTIONAL DESCRIPTION MS Detector Vacuum System and Inlet Gasses Hardware 7KHYDFXXPV\VWHPHYDFXDWHVWKHUHJLRQDURXQGWKH$3,VWDFN LRQRSWLFVPDVVDQDO\]HUDQGLRQGHWHFWLRQV\VWHP7KHSULQFLSDO FRPSRQHQWVRIWKHYDFXXPV\VWHPLQFOXGHWKHIROORZLQJ 9DFXXPPDQLIROG 7XUERPROHFXODUSXPS 5RWDU\YDQHSXPS &RQYHFWURQ JDXJH,RQJDXJH 7KHLQOHWJDVVHVKDUGZDUHFRQWUROVWKHIORZRIGDPSLQJJDV VKHDWKJDVDX[LOLDU\JDVDQGDLUGXULQJYHQWLQJLQWRWKH06 GHWHFWRU7KHLQOHWJDVVHVKDUGZDUHLQFOXGHVWKHIROORZLQJ FRPSRQHQWV 9HQWJDVYDOYH 'DPSLQJJDVLQOHWDVVHPEO\ 6KHDWKJDVYDOYH $X[LOLDU\JDVYDOYH A functional block diagram of the vacuum system and inlet gasses hardware is shown in )LJXUH. Finnigan: LCQ MS Detector Hardware Manual 2-31

82 FUNCTIONAL DESCRIPTION MS Detector Figure Functional block diagram of the vacuum system and inlet gasses hardware Vacuum Manifold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innigan: LCQ MS Detector Hardware Manual

83 FUNCTIONAL DESCRIPTION MS Detector 7ZRKLJKYROWDJHHOHFWULFDOIHHGWKURXJKVSDVVWKURXJKWKHYDFXXP PDQLIROG $IHHGWKURXJKIRUWKHKLJKYROWDJHIRUWKHFRQYHUVLRQG\QRGH SDVVHVWKURXJKWKHUHDUZDOO $IHHGWKURXJKIRUWKHULQJHOHFWURGH5)YROWDJHRIWKHPDVV DQDO\]HUSDVVHVWKURXJKWKHERWWRP $QLQOHWIRUWKHLQWURGXFWLRQRIDLULQWRWKHYDFXXPPDQLIROGIRU YHQWLQJWKHPDQLIROGSDVVHVWKURXJKWKHIURQWZDOORIWKHYDFXXP PDQLIROG7KHYDFXXPPDQLIROGDOVRKDVDQRSHQLQJIRUWKHLRQ JDXJH RING ELECTRODE RF VOLTAGE CONVERSION DYNODE O-RING ANALYZER REGION GUIDE HOLE (4 ) CAPILLARY-SKIMMER REGION LID FIRST OCTAPOLE REGION Figure Vacuum manifold (interior) Finnigan: LCQ MS Detector Hardware Manual 2-33

84 FUNCTIONAL DESCRIPTION MS Detector 7KHUHPRYDEOHWRSFRYHUSODWHRIWKHYDFXXPPDQLIROGKROGVWKH LRQRSWLFVPDVVDQDO\]HUDQGHOHFWURQPXOWLSOLHURQHSDUWRIWKH LRQGHWHFWLRQV\VWHP7KXVUHPRYDORIWKHWRSFRYHUSODWHDOORZV HDV\DFFHVVWRWKHVHDVVHPEOLHV7ZRKDQGOHVRQWKHWRSDQGIRXU JXLGHSRVWVRQWKHXQGHUVLGHRIWKHWRSFRYHUSODWHIDFLOLWDWHLWV UHPRYDODQGLQVWDOODWLRQ$QHOHFWULFDOO\FRQGXFWLYH2ULQJ SURYLGHVDYDFXXPWLJKWVHDOEHWZHHQWKHWRSFRYHUSODWHDQGWKH YDFXXPPDQLIROG7KHWRSFRYHUSODWHDQGLWVDWWDFKHGDVVHPEOLHV DUHVKRZQLQ)LJXUH HELIUM DAMPING GAS INLET MASS ANALYZE 4-PIN FEED- THROUGHS BAFFLE GUIDE POST ELECTRON MULTIPLIER 8-PIN FEED- THROUGH ION OPTICS (OCTAPOLES) Figure Top cover plate of the vacuum manifold (underside) and attached assemblies 2-34 Finnigan: LCQ MS Detector Hardware Manual

85 FUNCTIONAL DESCRIPTION MS Detector )LYHHOHFWULFDOIHHGWKURXJKVSDVVWKURXJKWKHWRSFRYHUSODWH $SLQIHHGWKURXJKIRUWKHILUVWRFWDSROHOHDGV $SLQIHHGWKURXJKIRUWKHVHFRQGRFWDSROHOHDGV $QSLQIHHGWKURXJKIRUWKHOHDGVWRWKHHQGFDSHOHFWURGHV DQGH[LWOHQVRIWKHPDVVDQDO\]HUDQGWKHLQWHURFWDSROHOHQV $IHHGWKURXJKIRUWKHKLJKYROWDJHIRUWKHFDWKRGHRIWKH HOHFWURQPXOWLSOLHU $IHHGWKURXJKIRUWKHLRQFXUUHQWVLJQDOIURPWKHDQRGHRIWKH HOHFWURQPXOWLSOLHU $QLQOHWIRUWKHLQWURGXFWLRQRIKHOLXPGDPSLQJJDVLQWRWKHPDVV DQDO\]HUSDVVHVWKURXJKWKHWRSFRYHUSODWH Turbomolecular Pump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innigan: LCQ MS Detector Hardware Manual 2-35

86 FUNCTIONAL DESCRIPTION MS Detector Rotary-Vane Pump $%DO]HUV812%URWDU\YDQHSXPSRUURXJKLQJSXPS HVWDEOLVKHVWKHYDFXXPQHFHVVDU\IRUWKHSURSHURSHUDWLRQRIWKH WXUERPROHFXODUSXPS7KHURWDU\YDQHSXPSDOVRHYDFXDWHVWKH FDSLOODU\VNLPPHUUHJLRQRIWKHYDFXXPPDQLIROG7KHSXPSKDV DPD[LPXPGLVSODFHPHQWRI30 m h and maintains a minimum pressure of approximately 100 Pa (1 Torr). 7KHURWDU\YDQHSXPSLVFRQQHFWHGWRWKHWXUERPROHFXODUSXPSE\ DVHFWLRQRILQ,'UHLQIRUFHG39&WXELQJ7KHSRZHUFRUGRIWKH URWDU\YDQHSXPSLVSOXJJHGLQWRWKHRXWOHWODEHOHG5RXJK3XPS RQWKHSRZHUSDQHO6HH)LJXUHRQSDJH7KLVRXWOHW VXSSOLHVSRZHUWRWKHSXPSDQGLVFRQWUROOHGE\WKHPDLQSRZHU FLUFXLWEUHDNHUVZLWFKDQGQRWE\WKHHOHFWURQLFVVHUYLFHVZLWFK &DXWLRQ$OZD\VSOXJWKHURWDU\YDQHSXPSSRZHUFRUGLQWRWKH RXWOHWODEHOHG5RXJK3XPSRQWKHULJKWVLGHRIWKH06GHWHFWRU 1HYHUSOXJLWLQWRDZDOORXWOHW Convectron Gauge 7KH&RQYHFWURQJDXJHPHDVXUHVWKHSUHVVXUHLQWKHFDSLOODU\ VNLPPHUUHJLRQRIWKHYDFXXPPDQLIROGDQGWKHIRUHOLQHZKLFK FRQQHFWVWKHWXUERPROHFXODUSXPSDQGWKHURWDU\YDQHSXPS 7KHSUHVVXUHPHDVXUHGE\WKH&RQYHFWURQJDXJHLVPRQLWRUHGE\ WKH6\VWHP&RQWURO3&%7KH6\VWHP&RQWURO3&%GHWHFWV ZKHWKHUWKHIRUHOLQHSUHVVXUHLVWRRKLJKIRUWKHSURSHURSHUDWLRQ RIWKHWXUERPROHFXODUSXPS3RZHUWRWKHWXUERPROHFXODUSXPSLV UHPRYHGLIWKHIRUHOLQHSUHVVXUHLVWRRKLJK Ion Gauge The pressure in the analyzer region of the vacuum manifold is measured by a Granville-Phillips Š 342 Œ mini ion gauge. The ion gauge produces energetic electrons that cause the ionization of molecules in the ion gauge. Positive ions formed in the ion gauge are attracted to a collector. The collector current is related to the 2-36 Finnigan: LCQ MS Detector Hardware Manual

87 FUNCTIONAL DESCRIPTION MS Detector pressure in the vacuum manifold. The ion gauge is also involved in vacuum protection Vent Valve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amping Gas Inlet Assembly 7KHGDPSLQJJDVLQOHWDVVHPEO\FRQWUROVWKHIORZRIKHOLXPLQWR LQSRUWODEHOHG+HOLXP,QRQWKHOHIWVLGHRIWKH06GHWHFWRU 6HH)LJXUHRQSDJH/&4UHJXODWHVWKHIORZRIKHOLXP E\XVHRIDFDSLOODU\UHVWULFWRUDQGDSUHVVXUHUHJXODWRURQWKH KHOLXPOLQH7KHKHOLXPHQWHUVWKHPDVVDQDO\]HUWKURXJKD QLSSOHRQWKHH[LWHQGFDSHOHFWURGH +HOLXPLQWKHPDVVDQDO\]HUFDYLW\GDPSHQVLRQLFPRWLRQDQG LPSURYHVWKHSHUIRUPDQFHRIWKH06GHWHFWRU5HIHUWRWKHWRSLF +HOLXP'DPSLQJ*DVLQWKH0DVV$QDO\]HU&DYLW\RQSDJH Finnigan: LCQ MS Detector Hardware Manual 2-37

88 FUNCTIONAL DESCRIPTION MS Detector 1RWH+HOLXPGDPSLQJJDVFRQWLQXHVWRIORZWRWKHPDVVDQDO\]HU HYHQDIWHUWKH/&4LVSRZHUHGRIIRUSODFHGLQ6WDQGE\7RVDYH KHOLXPZKHQWKH/&4LVQRWRSHUDWLRQDOWXUQRIIWKHKHOLXPIORZ DWWKHWDQN Sheath Gas Valve 7KHVKHDWKJDVYDOYHFRQWUROVWKHIORZRIVKHDWKJDVQLWURJHQ SXULW\HQWHUVWKH06GHWHFWRUWKURXJKDLQSRUWODEHOHG 1LWURJHQ,QRQWKHOHIWVLGHRIWKH06GHWHFWRU6HH)LJXUHRQ SDJH7KHVKHDWKJDVSUHVVXUHLVUHJXODWHGE\DYDOYHWKDWLV FRQWUROOHGE\WKHGDWDV\VWHP<RXFDQVHWWKHVKHDWKJDVIORZ UDWHWRLQDUELWUDU\XQLWVLQWKH(6,6RXUFHDQG$3&, 6RXUFHGLDORJER[HVIURPWKH7XQH3OXVZLQGRZ7KHVKHDWKJDV HQWHUVWKH$3,VRXUFHWKURXJKLQ,'WXELQJ,QWKHHYHQWWKDWVKHDWKJDVIORZLVORZHUWKDQUHTXLUHGIRU RSHUDWLRQ/&4GLVSOD\VDPHVVDJHDQGFKDQJHVV\VWHPVWDWXVWR 6WDQGE\ Auxiliary Gas Valve 7KHDX[LOLDU\JDVYDOYHFRQWUROVWKHIORZRIDX[LOLDU\JDV QLWURJHQLQWRWKH$3,VRXUFH'U\QLWURJHQ±SVLJ LQSRUWODEHOHG1LWURJHQ,QRQWKHOHIWVLGHRIWKH06GHWHFWRU 7KHDX[LOLDU\JDVSUHVVXUHLVUHJXODWHGE\DIORZYDOYHWKDWLV FRQWUROOHGE\WKHGDWDV\VWHP<RXFDQVHWWKHDX[LOLDU\JDVIORZ UDWHWRLQDUELWUDU\XQLWVLQWKH(6,6RXUFHDQG$3&, 6RXUFHGLDORJER[HV7KHDX[LOLDU\JDVHQWHUVWKH$3,VRXUFH WKURXJK LQ,'WXELQJ 2-38 Finnigan: LCQ MS Detector Hardware Manual

89 FUNCTIONAL DESCRIPTION MS Detector Cooling Fans )LYHIW PLQ IDQVSURYLGHFRROLQJIRUWKH06GHWHFWRU2QH IDQFRROVWKH5)YROWDJHFRLO7ZRIDQVFRROWKHHOHFWURQLFVLQWKH WRZHU2QHIDQFRROVWKHHOHFWURQLFVLQWKHHPEHGGHGFRPSXWHU 2QHIDQFRROVWKHWXUERPROHFXODUSXPS7KHH[KDXVWDLULV H[SHOOHGIURPWKHYHQWVORWVRQWKHVLGHVRIWKH06GHWHFWRU 9GFSRZHUWRWKH5)FRLOIDQWKHHPEHGGHGFRPSXWHUIDQ DQGWKHWRZHUIDQVLVSURYLGHGE\WKH9±99GF VZLWFKLQJSRZHUVXSSO\9GFSRZHUWRWKHWXUERPROHFXODU SXPSIDQLVSURYLGHGE\WKH9NHHSDOLYHSRZHUVXSSO\LQWKH 3RZHU0RGXOH &DXWLRQ7RHQVXUHSURSHUFRROLQJWKH06GHWHFWRUPXVWDOZD\V EHRSHUDWHGZLWKLWVFRYHUVLQSODFH Finnigan: LCQ MS Detector Hardware Manual 2-39

90 FUNCTIONAL DESCRIPTION MS Detector Electronic Assemblies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ower Module and Power Distribution 7KH3RZHU0RGXOHSURYLGHVSRZHUWRWKHYDFXXPV\VWHPDQG FRQYHUWVWKHOLQHSRZHUWRYROWDJHVQHHGHGE\WKH06GHWHFWRU 7KH3RZHU0RGXOHLQFOXGHVWKHIROORZLQJFRPSRQHQWV 0DLQSRZHUFLUFXLWEUHDNHU /LQHILOWHU 9NHHSDOLYHSRZHUVXSSO\ (OHFWURQLFVVHUYLFHVZLWFK /LQHYROWDJHVZLWFK 7UDQVIRUPHU $IXQFWLRQDOEORFNGLDJUDPRIWKH3RZHU0RGXOHDQG06GHWHFWRU SRZHUGLVWULEXWLRQLVVKRZQLQ)LJXUH 2-40 Finnigan: LCQ MS Detector Hardware Manual

91 FUNCTIONAL DESCRIPTION MS Detector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innigan: LCQ MS Detector Hardware Manual 2-41

92 FUNCTIONAL DESCRIPTION MS Detector Figure Functional block diagram of the Power Module and power distribution of the MS detector 7KHHOHFWURQLFVVHUYLFHVZLWFKLVDFLUFXLWEUHDNHUWKDWDOORZV \RXWRVHUYLFHWKHQRQYDFXXPV\VWHPFRPSRQHQWVRIWKH06 GHWHFWRUZLWKWKHYDFXXPV\VWHPVWLOOLQRSHUDWLRQ6HH)LJXUH IRUWKHORFDWLRQRIWKHHOHFWURQLFVVHUYLFHVZLWFK,QWKH6HUYLFH 2))2SRVLWLRQWKHVZLWFKUHPRYHVSRZHUWRDOOFRPSRQHQWVRI WKH06GHWHFWRURWKHUWKDQWKHYDFXXPV\VWHP,QWKH1RUPDO 2-42 Finnigan: LCQ MS Detector Hardware Manual

93 FUNCTIONAL DESCRIPTION MS Detector 21_SRVLWLRQSRZHULVVXSSOLHGWRDOOFRPSRQHQWVRIWKH06 GHWHFWRU 1RWH)RUHPHUJHQF\VKXWRIIRIDOOSRZHUWRWKH06GHWHFWRUSODFH WKHPDLQSRZHUFLUFXLWEUHDNHUVZLWFKLQWKH2II2SRVLWLRQ'R QRWXVHWKHHOHFWURQLFVVHUYLFHVZLWFKWRUHPRYHSRZHUWRWKH V\VWHPLQDQHPHUJHQF\ $IWHUWKHHOHFWURQLFVVHUYLFHVZLWFKSRZHUJRHVWRWKH9±9 9VZLWFKLQJSRZHUVXSSO\WKH99VZLWFKLQJSRZHU VXSSO\DQGWKHOLQHYROWDJHVZLWFK 7KH9±99GFVZLWFKLQJSRZHUVXSSO\SURYLGHV 9GF±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innigan: LCQ MS Detector Hardware Manual 2-43

94 FUNCTIONAL DESCRIPTION MS Detector System Control PCB 7KH6\VWHP&RQWURO3&%LVWKHSULQFLSDO3&%IRUFRQWUROOLQJDQG PRQLWRULQJWKHRSHUDWLRQRIWKH06GHWHFWRU7KH6\VWHP&RQWURO 3&%LVWKHODUJHERDUGWKDWLVORFDWHGRQWKHULJKWVLGHRIWKH06 GHWHFWRU7KHPDQ\LQWHUFRQQHFWLRQVRIWKH6\VWHP&RQWURO3&% ZLWKRWKHUFRPSRQHQWVRIWKH06GHWHFWRUFDQEHVHHQLQ )LJXUHWKURXJK)LJXUH 7KH6\VWHP&RQWURO3&%FRQWDLQVWKHIROORZLQJFRPSRQHQWV ±9GF±9GF±9GF±9GF9GF9DF 9GFDQG9DFSRZHUVXSSOLHV 'FYROWDJHFRQWUROFLUFXLWU\ 'LYHUWLQMHFWYDOYHFRQWUROFLUFXLW $3,VRXUFHFRQWUROFLUFXLW (OHFWURQPXOWLSOLHUFRQWUROFLUFXLW &RQYHUVLRQG\QRGHFRQWUROFLUFXLW +HDWHGFDSLOODU\KHDWHUVHQVRUFRQWUROFLUFXLW $3&,YDSRUL]HUKHDWHUVHQVRUFRQWUROFLUFXLW,RQJDXJHFRQWUROFLUFXLW &RQYHFWURQJDXJHFRQWUROFLUFXLW 9DFXXPSURWHFWLRQFLUFXLWU\ RF voltage control circuitry 6ZLWFKLQJSRZHUVXSSO\RXWSXWV 7HPSHUDWXUHPRQLWRULQJFLUFXLWU\ 'LDJQRVWLFFLUFXLWU\,QWHUIDFHZLWKWKHHPEHGGHGFRPSXWHU 7KH±9GFSRZHUVXSSO\SURYLGHVSRZHUIRUWKHGFYROWDJHV WKDWDUHDSSOLHGWRWKHKHDWHGFDSLOODU\RFWDSROHVDQGPDVV DQDO\]HUHOHFWURGHV7KH±9GFSRZHUVXSSO\UHFHLYHV9 DFIURPWKHWRURLGDOWUDQVIRUPHULQWKH3RZHU0RGXOH 2-44 Finnigan: LCQ MS Detector Hardware Manual

95 FUNCTIONAL DESCRIPTION MS Detector 7KH±9GFSRZHUVXSSO\SURYLGHVSRZHUIRUWKHFLUFXLWU\ WKDWSURYLGHVYROWDJHWRWKHLQWHURFWDSROHOHQV7KH±9GF SRZHUVXSSO\UHFHLYHV9DFIURPWKHWRURLGDOWUDQVIRUPHULQ WKH3RZHU0RGXOH 7KH±9GFSRZHUVXSSO\SURYLGHVSRZHUIRUWKHGFYROWDJH WKDWLVDSSOLHGWRWKHWXEHOHQV7KH±9GFSRZHUVXSSO\ UHFHLYHV9DFIURPWKHWRURLGDOWUDQVIRUPHULQWKH3RZHU 0RGXOH 7KH±9GFSRZHUVXSSO\SURYLGHVSRZHUIRUWKH5)GHWHFWRULQ WKH5)9ROWDJH&RQWURO3&%7KH±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he electron multiplier control circuit sends a signal to the electron multiplier power supply that is proportional to the voltage to be applied to the electron multiplier cathode. It also reads back a signal that is proportional to the actual voltage applied to the electron multiplier cathode. The electron multiplier control circuit lowers the electron multiplier voltage when mass analysis is not occurring. Finnigan: LCQ MS Detector Hardware Manual 2-45

96 FUNCTIONAL DESCRIPTION MS Detector The conversion dynode control circuit controls and monitors the polarity of the 15 kv potential that is applied to the conversion dynode. The heated capillary heater/sensor control circuit monitors the temperature of the heated capillary via a platinum probe temperature sensor. It also provides the voltage needed by the heater in the heated capillary. The APCI vaporizer heater/sensor control circuit controls the temperature of the APCI vaporizer via a thermocouple sensor. It also provides 230 V ac line voltage to the heater in the APCI vaporizer. The ion gauge control circuit controls the ion gauge and reads back the pressure signal. (The ion gauge measures the pressure in the analyzer region of the vacuum manifold.) The Convectron gauge control circuit controls the Convectron gauge and reads back the pressure signal. (The Convectron gauge measures the pressure in the foreline and the capillary-skimmer region of the vacuum manifold.) 7KHYDFXXPSURWHFWLRQFLUFXLWU\PRQLWRUVWKHSUHVVXUHLQWKH IRUHOLQHEHWZHHQWKHWXUERPROHFXODUSXPSDQGWKHURWDU\YDQH SXPSDVPHDVXUHGE\WKH&RQYHFWURQJDXJHDQGLQWKHDQDO\]HU UHJLRQRIWKHYDFXXPPDQLIROGDVPHDVXUHGE\WKHLRQJDXJH 5HIHUWRWKHWRSLF9DFXXP6\VWHP(OHFWURQLF$VVHPEOLHVRQ SDJHIRUPRUHLQIRUPDWLRQRQYDFXXPSURWHFWLRQ The RF voltage control circuitry controls and monitors the RF Voltage Amplifier PCB, Waveform Amplifier PCB, and RF Voltage Control PCB. The switching power supply outputs distribute 9±9DQG 9GFSRZHUWRYDULRXV3&%VDQGPRGXOHVRIWKH06GHWHFWRU The temperature monitoring circuitry monitors the temperatures at the RF Voltage Control PCB, Analyzer PCB, RF Voltage Amplifier PCB, and System Control PCB. The diagnostic circuitry monitors the outputs of various components and circuits on the LCQ. Information on voltages, currents, temperatures, flow rates, logic, etc. is sent to the data system, where it can be accessed via the Tune Plus Status view and the diagnostics program. 7KHHPEHGGHGFRPSXWHULQWHUIDFHLVDKLJKVSHHGVHULDOOLQH WKDWFRQQHFWVWKH6\VWHP&RQWURO3&%ZLWKWKH&RQWURO' Finnigan: LCQ MS Detector Hardware Manual

97 FUNCTIONAL DESCRIPTION MS Detector GLJLWDOVLJQDOSURFHVVRU3&%LQWKHHPEHGGHGFRPSXWHU7KH 6\VWHP&RQWURO3&%FRPPXQLFDWHVZLWKWKHGDWDV\VWHP FRPSXWHUYLDWKHHPEHGGHGFRPSXWHU Vacuum System Electronic Assemblies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innigan: LCQ MS Detector Hardware Manual 2-47

98 FUNCTIONAL DESCRIPTION MS Detector Figure Functional block diagram of the vacuum system electronic assemblies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innigan: LCQ MS Detector Hardware Manual

99 FUNCTIONAL DESCRIPTION MS Detector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innigan: LCQ MS Detector Hardware Manual 2-49

100 FUNCTIONAL DESCRIPTION MS Detector RF/Waveform Voltage Generation Electronic Assemblies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he Waveform Amplifier PCB receives and amplifies the waveform produced by the Waveform DDS PCB. The Waveform Amplifier PCB also receives a sine wave reference signal from the Waveform DDS PCB that it uses to produce the resonance excitation and resonance ejection RF voltages Finnigan: LCQ MS Detector Hardware Manual

101 FUNCTIONAL DESCRIPTION MS Detector The RF Voltage Amplifier PCB provides the ULQJHOHFWURGHRF primary voltage for the RF voltage coil. The RF Voltage Amplifier PCB receives a sine wave reference signal from the Waveform DDS PCB. The Low Pass Filter PCB removes second and third harmonics from the ring electrode RF voltage. The dc ring filter filters the dc offset voltage that is applied to the ring electrode. The RF voltage coil amplifies the primary voltage from the RF Voltage Amplifier PCB to produce a secondary voltage of 0 to 8500 V ac (peak) that is supplied to the ring electrode of the mass analyzer. Also, the dc offset voltage that is applied to the ring electrode is added to the ring electrode RF voltage at the RF voltage coil. The RF Voltage Control PCB detects and controls the amplitude of the RF voltage that is applied to the ring electrode. The RF Voltage Control PCB includes the mass control circuit, and it resides in a thermally stable housing, which helps to prevent mass calibration drift. 7KH$QDO\]HU$X[LOLDU\3&%UHFHLYHVWKHRFWDSROH5)sine wave reference signal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innigan: LCQ MS Detector Hardware Manual 2-51

102 FUNCTIONAL DESCRIPTION MS Detector Figure RF/waveform voltage generation electronic assemblies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innigan: LCQ MS Detector Hardware Manual

103 FUNCTIONAL DESCRIPTION MS Detector Ion Detection System Electronic Assemblies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innigan: LCQ MS Detector Hardware Manual 2-53

104 FUNCTIONAL DESCRIPTION MS Detector Figure Functional block diagram of the ion detection system electronic assemblies Embedded Computer Electronic Assemblies 7KHHPEHGGHGFRPSXWHULVDFRPSXWHUZLWKLQWKH06GHWHFWRU WKDWFRRUGLQDWHVLQVWUXPHQWFRQWUROZDYHIRUPDQG5)YROWDJH JHQHUDWLRQGDWDDFTXLVLWLRQDQGFRPPXQLFDWLRQZLWKWKHGDWD V\VWHPDQGH[WHUQDOGHYLFHV$IXQFWLRQDOEORFNGLDJUDPRIWKH HPEHGGHGFRPSXWHULVVKRZQLQ)LJXUH7KHHPEHGGHG FRPSXWHUFRQWDLQVWKHIROORZLQJFRPSRQHQWV,6$EXV$7EDFNSODQH &383&% 6HULDO,23&% :DYHIRUP''63&% &RQWURO'633&% $FTXLVLWLRQ'633&%,(((,23&%RSWLRQDO 2-54 Finnigan: LCQ MS Detector Hardware Manual

105 FUNCTIONAL DESCRIPTION MS Detector (WKHUQHW3&% Figure Functional block diagram of the embedded computer, showing communication with the MS detector and external devices Finnigan: LCQ MS Detector Hardware Manual 2-55

106 FUNCTIONAL DESCRIPTION MS Detector 7KH,6$LQGXVWU\VWDQGDUGDUFKLWHFWXUHEXV$7EDFNSODQH SURYLGHVSRZHUWRDQGFRPPXQLFDWLRQEHWZHHQWKH3&%VLQWKH HPEHGGHGFRPSXWHU 7KH&383&%LVWKHFHQWUDOSURFHVVLQJXQLWIRUWKHHPEHGGHG FRPSXWHU7KH&383&%FRQWDLQV0E\WHVRI5$06RIWZDUH WRUXQWKHHPEHGGHGFRPSXWHUZKLFKLVFRQWDLQHGLQUHDGRQO\ PHPRU\RQWKH&383&%LVORDGHGLQWR5$0RQERRWXS$IWHU ERRWXSVRIWZDUHWRUXQWKH/&4LVWUDQVIHUHGIURPWKHGDWD V\VWHPWRWKHHPEHGGHGFRPSXWHURYHUDQ(WKHUQHW<RXFDQ UHERRWWKH&383&%DQGGRZQORDGWKH/&4VRIWZDUHIURPWKH GDWDV\VWHPE\SUHVVLQJWKHUHVHWEXWWRQRQWKHSRZHUSDQHO 7KH6HULDO,23&%SURYLGHVVHULDO56FRPPXQLFDWLRQZLWK WKH7XUERPROHFXODU3XPS&RQWUROOHUSRUWV\ULQJHSXPSSRUW DX[LOLDU\GHYLFHSRUW899,6GHWHFWRUSRUWDXWRVDPSOHU SRUWDQG/&SRUW6HULDOOLQHVIURPH[WHUQDOGHYLFHVSOXJ LQWRWKHVHULDOSRUWVRQWKH,2SDQHO6HH)LJXUH 7KH:DYHIRUP''6GLUHFWGLJLWDOV\QWKHVL]HU3&%SURYLGHV WKHUHIHUHQFH sine wave or ac YROWDJHVIRUWKHUHVRQDQFHH[FLWDWLRQ 5)YROWDJHUHVRQDQFHHMHFWLRQ5)YROWDJHLRQLQMHFWLRQZDYHIRUP YROWDJHDQGLRQLVRODWLRQZDYHIRUPYROWDJH7KHVHYROWDJHVDUH VHQWWRWKH:DYHIRUP$PSOLILHU3&%LQWKH06GHWHFWRU,WDOVR SURYLGHVWKHUHIHUHQFHYROWDJHIRUWKHULQJHOHFWURGH5)YROWDJH ZKLFKLVVHQWWRWKHRF Voltage Amp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oltage AmpOLILHU3&% :DYHIRUP$PSOLILHU3&%DQG$QDO\]HU$X[LOLDU\3&%7KH 2-56 Finnigan: LCQ MS Detector Hardware Manual

107 FUNCTIONAL DESCRIPTION MS Detector :DYHIRUP''63&%&RQWURO'633&%DQG$FTXLVLWLRQ'633&% FRPPXQLFDWHZLWKHDFKRWKHURYHUDORFDOEXV 7KHRSWLRQDO,(((,23&%SURYLGHV*3,%FRPPXQLFDWLRQ ZLWK+HZOHWW3DFNDUGGHYLFHV7KH*3,%OLQHIURPH[WHUQDO GHYLFHVSOXJVLQWRWKH,(((*3,%SRUWRQWKH,2SDQHO6HH )LJXUH 7KH(WKHUQHW3&%SURYLGHV(WKHUQHWFRPPXQLFDWLRQEHWZHHQWKH HPEHGGHGFRPSXWHUDQGWKHGDWDV\VWHPRYHUDWKLQZLUH(WKHUQHW FDEOH SERIAL (RS 232) PORTS CONTACT CLOSURE AND ANALOG INPUT PORTS NITROGEN GAS INLET HELIUM GAS INLET IEEE 488 (GPIB) PORT Figure I/O panel Finnigan: LCQ MS Detector Hardware Manual 2-57

108 FUNCTIONAL DESCRIPTION Data System 2.6 Data System 7KHGDWDV\VWHPFRQWUROVDQGPRQLWRUVWKH/&4V\VWHP7KH GDWDV\VWHPDOVRSURFHVVHVGDWDWKDWLVDFTXLUHGE\WKH/&4 V\VWHP7KHGDWDV\VWHPLVFRPSRVHGRIWKHIROORZLQJ &RPSXWHUKDUGZDUH &RPSXWHUVRIWZDUH 'DWDV\VWHP06GHWHFWRULQWHUIDFH 'DWDV\VWHPORFDODUHDQHWZRUNLQWHUIDFH 3ULQWHU Computer Hardware 7KHGDWDV\VWHPFRPSXWHULVD*DWHZD\ SHUVRQDOFRPSXWHU,WVPDMRUIHDWXUHVLQFOXGH,QWHO Pentium processor 0%RIUDQGRPDFFHVVPHPRU\5$0 ELW3&,,6$V\VWHPDUFKLWHFWXUH 6&6,KDUGGLVNGULYH &'520GULYH 3ULPDU\(WKHUQHWDGDSWHUGDWDV\VWHPWR06GHWHFWRU 6HFRQGDU\(WKHUQHWDGDSWHUGDWDV\VWHPWRORFDODUHD QHWZRUN 0%LQGLVNHWWHGULYH 9LGHR*UDSKLFVFDUGZLWK0%5$0 LQ UHVROXWLRQ69*$FRORUPRQLWRU.H\ERDUGDQGPRXVH )RUPRUHLQIRUPDWLRQDERXWWKHFRPSXWHUUHIHUWRWKHPDQXDOV WKDWFRPHZLWKWKHFRPSXWHU 2-58 Finnigan: LCQ MS Detector Hardware Manual

109 FUNCTIONAL DESCRIPTION Data System Computer Software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innigan: LCQ MS Detector Hardware Manual 2-59

110 FUNCTIONAL DESCRIPTION Data System Data System / MS Detector Interface 7KHGDWDV\VWHPFRPSXWHUFRQWDLQVDQ(WKHUQHWDGDSWHUFDOOHG WKHSULPDU\(WKHUQHWDGDSWHUWKDWLVGHGLFDWHGWRGDWD V\VWHP06GHWHFWRUFRPPXQLFDWLRQV7KH(WKHUQHWDGDSWHURQ WKHLQVWUXPHQWUHVLGHVLQWKHHPEHGGHGFRPSXWHUER[$WKLQZLUH (WKHUQHWFDEOHFRQQHFWVWKHSULPDU\(WKHUQHWDGDSWHURIWKHGDWD V\VWHPZLWKWKH(WKHUQHWFRQQHFWRURQWKHSRZHUSDQHORIWKH06 GHWHFWRUVHH)LJXUH Data System / Local Area Network Interface 7KHGDWDV\VWHPFRPSXWHUFRQWDLQVDVHFRQG(WKHUQHWDGDSWHU FDOOHGWKHVHFRQGDU\(WKHUQHWDGDSWHU7KHVHFRQGDU\(WKHUQHW DGDSWHULVQRWLQYROYHGLQGDWDV\VWHP06GHWHFWRU FRPPXQLFDWLRQV<RXFDQXVHWKHVHFRQGDU\(WKHUQHWDGDSWHUWR FRQQHFW\RXU/&43&WRDORFDODUHDQHWZRUN Printer $KLJKUHVROXWLRQODVHUSULQWHULVLQFOXGHGZLWKWKH/&47KH SULQWHUFRPPXQLFDWHVZLWKWKH3&YLDDSDUDOOHOSRUW/37 5HIHUWRWKHPDQXDOVXSSOLHGE\WKHPDQXIDFWXUHUIRUGHWDLOV DERXWWKHSULQWHU <RXVHWXSWKHSULQWHUIURPWKH3ULQW6HWXSGLDORJER[7RRSHQ WKH3ULQW6HWXSGLDORJER[FKRRVHFile Print SetupLQDQ\ ZLQGRZ 2-60 Finnigan: LCQ MS Detector Hardware Manual

111 PREPARING FOR DAILY OPERATION Contents Contents 35(3$5,1*)25'$,/<23(5$7,21 &KHFNLQJWKH'LVN6SDFH &KHFNLQJWKH+HOLXPDQG1LWURJHQ6XSSOLHV &KHFNLQJWKH6\VWHP9DFXXP/HYHOV 3ULQWLQJD+DUG&RS\RIWKH6WDWXV9LHZ List of Tables 7DEOH7\SLFDO3UHVVXUH5HDGLQJV Finnigan: LCQ MS Detector Hardware Manual 3-i

112 PREPARING FOR DAILY OPERATION 3. PREPARING FOR DAILY OPERATION 7KLVFKDSWHURXWOLQHVWKHFKHFNVRIWKH/&4V\VWHPWKDWVKRXOGEH SHUIRUPHGHYHU\GD\EHIRUH\RXEHJLQ\RXUILUVWDQDO\VLV7KH IROORZLQJFKHFNVVKRXOGEHSHUIRUPHGHYHU\GD\ &KHFNGLVNVSDFHRQWKHGDWDV\VWHP &KHFNKHOLXPDQGQLWURJHQJDVSUHVVXUHV &KHFNV\VWHPYDFXXPOHYHOV 3ULQWDKDUGFRS\RIWKH7XQH3OXV6WDWXVYLHZ 1RWH<RXGRQRWQHHGWRFDOLEUDWHRUWXQHWKH/&4DVSDUWRI\RXU GDLO\URXWLQH &DOLEUDWLRQSDUDPHWHUVDUHLQVWUXPHQWSDUDPHWHUVZKRVH YDOXHVGRQRWYDU\ZLWKWKHW\SHRIH[SHULPHQW<RXQHHGWR FDOLEUDWHWKH/&4SHUKDSVRQFHDPRQWKDQGFKHFNWKHFDOLEUDWLRQ RQFHDZHHN5HIHUWRWKH/&42SHUDWRUªV0DQXDOIRUD SURFHGXUHIRUFDOLEUDWLQJWKH/&4 7XQHSDUDPHWHUVDUHLQVWUXPHQWSDUDPHWHUVZKRVHYDOXHVYDU\ ZLWKWKHW\SHRIH[SHULPHQW<RXQHHGWRWXQHWKH/&4RUFKDQJH WKH7XQH0HWKRGZKHQHYHU\RXFKDQJHWKHW\SHRIH[SHULPHQW 5HIHUWRWKH/&42SHUDWRUªV0DQXDOIRUSURFHGXUHVIRUWXQLQJ WKH/&4LQWKH(6,RU$3&,PRGH1RWHWKDW/&4FRPHVZLWK VHYHUDOVWDQGDUG7XQH0HWKRGVVSHFLILFIRUYDULRXVH[SHULPHQWDO FRQGLWLRQVVRWKDWWXQLQJLVRIWHQQRWUHTXLUHGIRUPDQ\W\SHVRI H[SHULPHQWV Finnigan: LCQ MS Detector Hardware Manual 3-1

113 PREPARING FOR DAILY OPERATION Checking the Disk Space 3.1 Checking the Disk Space <RXVKRXOGYHULI\RQDUHJXODUEDVLVWKDW\RXUKDUGGLVNGULYHKDV HQRXJKIUHHVSDFHIRUGDWDDFTXLVLWLRQ7KHDPRXQWRIDYDLODEOH GLVNVSDFHLVVKRZQLQWKH&KHFN'LVN6SDFHGLDORJER[7R GHWHUPLQHWKHDPRXQWRIDYDLODEOHGLVNVSDFHSURFHHGDVIROORZV )URPWKH1DYLJDWRUZLQGRZFKRRVHSamples Edit Sample ListWRRSHQWKH6DPSOH/LVWZLQGRZ &KRRVHActions Check Disk Space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innigan: LCQ MS Detector Hardware Manual

114 PREPARING FOR DAILY OPERATION Checking the Helium and Nitrogen Supplies 3.2 Checking the Helium and Nitrogen Supplies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lJOLFKYRU%HJLQQGHVQRUPDOHQ%HWULHEV VLFKHUGD 6LHJHQ JHQG6WLFNVWRIII U,KUH$WPRVSKlUHQGUXFN,RQLVDWLRQVTXHOOH$3,KDEHQ:HQQGHU6WLFNVWRIIQLFKWDXVUHLFKW VFKDOWHWGDV/&4GHQ06'HWHNWRUDXWRPDWLVFKDXI$86XP]X YHUKLQGHUQGD /XIWVDXHUVWRIILQGLH,RQHQTXHOOHHLQGULQJW 6DXHUVWRIILQGHU,RQHQTXHOOHNDQQEHLHLQJHVFKDOWHWHP06 'HWHNWRUHLQH*HIDKUGDUVWHOOHQ$X HUGHPN QQHQELHP $EVFKDOWHQGHV06'HWHNWRUVZlKUHQGHLQHU$QDO\VH'DWHQ YHUORUHQJHKHQ Finnigan: LCQ MS Detector Hardware Manual 3-3

115 PREPARING FOR DAILY OPERATION Checking the Helium and Nitrogen Supplies $9(57,66(0(177RXVOHVMRXUVDYDQWGªXWLOLVHUOªDSSDUHLO YpULILH]OHQLYHDXGªD]RWHGHODVRXUFHGªLRQLVDWLRQjODSUHVVLRQ DWPRVSKpULTXH(QHIIHWVLYRXVWRPEH]jFRXUWGªD]RWH/&4 GpVDFWLYHDXWRPDWLTXHPHQWOHGpWHFWHXUGH60SRXUHPSrFKHUTXH ODVRXUFHGªLRQVQHVRLWFRQWDPLQpHSDUOªR[\JqQHSUpVHQWGDQVOªDLU 7RXWHSUpVHQFHGªR[\JqQHGDQVODVRXUFHGªLRQVORUVTXHOHGpWHFWHXU GH60HVWDFWLYpSHXWFRPSRUWHUGHVULVTXHV(QRXWUHVL/&4 GpVDFWLYDLWOHGpWHFWHXUGH60SHQGDQWXQHVpULHGªDQDO\VHVYRXV SRXUULH]SHUGUHGHVGRQQpHV 3-4 Finnigan: LCQ MS Detector Hardware Manual

116 PREPARING FOR DAILY OPERATION Checking the System Vacuum Levels 3.3 Checking the System Vacuum Levels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etup Vacuum &RPSDUHWKHFXUUHQWYDOXHVRIWKHSUHVVXUHVLQWKHYDFXXP PDQLIROGZLWKWKHYDOXHVOLVWHGLQ7DEOHDQGZLWKYDOXHVIURP 6WDWXVYLHZVWKDW\RXSUHYLRXVO\SULQWHGRXW,IWKHFXUUHQW YDOXHVDUHKLJKHUWKDQQRUPDO\RXPD\KDYHDQDLUOHDN Finnigan: LCQ MS Detector Hardware Manual 3-5

117 PREPARING FOR DAILY OPERATION Checking the System Vacuum Levels Table 3-1. Typical Pressure Readings Conditions Convectron gauge reading (foreline, capillary-skimmer region) Ion gauge reading (analyzer region) Helium on, heated capillary open (200 C) 0.9 to 1.1 Torr 2.0 x 10-5 to 2.5 x 10-5 Torr Helium on, heated capillary open (25 C) 1.2 Torr (or slightly higher) 2.9 x 10-5 Torr Helium on, heated capillary closed 2 x 10-3 to 5 x 10-3 Torr 1.8 x 10-5 Torr Helium off, heated capillary open (200 C) 0.9 to 1.1 Torr 7 x 10-6 Torr Helium off, heated capillary open (25 C) 1.2 Torr (or slightly higher) 9 x 10-6 Torr Helium off, heated capillary closed 2 x 10-3 to 5 x 10-3 Torr 2.0 x 10-6 Torr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innigan: LCQ MS Detector Hardware Manual

118 PREPARING FOR DAILY OPERATION Printing a Hard Copy of the Status View 3.4 Printing a Hard Copy of the Status View 7KH7XQH3OXV6WDWXVYLHZOLVWVWKHFXUUHQWYDOXHVRIWKH LQVWUXPHQWSDUDPHWHUV,WLVDJRRGLGHDWRSULQWRUVDYHLQDILOH DKDUGFRS\RIWKHVWDWXVYLHZRQDGDLO\EDVLV7KLVSURFHGXUH DOORZV\RXWRGHWHFWSUREOHPVE\PRQLWRULQJFKDQJHVLQWKH LQVWUXPHQWSDUDPHWHUV 7RSULQWDFRS\RIWKH6WDWXVYLHZSURFHHGDVIROORZV 1. From the Tune Plus window, choose Control On (or click on the On/Standby button) to turn on voltages to the API source, ion optics, mass analyzer, and ion detection system. 2. Choose View Display Status View (or click on the Display Status View button on the button bar). The Status view appears in the Tune Plus window. 3. Click on the Status view to make it the active window. 4. Print a hard copy of the Status view by choosing File Print. &KRRVHControl StandbyWRWXUQRIIYROWDJHVWRWKH$3, VRXUFHLRQRSWLFVPDVVDQDO\]HUDQGLRQGHWHFWLRQV\VWHP Finnigan: LCQ MS Detector Hardware Manual 3-7

119 CHANGING API PROBE ASSEMBLIES Contents Contents &+$1*,1*$3,352%($66(0%/,(6 5HPRYLQJWKH$3&,3UREH$VVHPEO\,QVWDOOLQJWKH(6,3UREH$VVHPEO\ 5HPRYLQJWKH(6,3UREH$VVHPEO\,QVWDOOLQJWKH$3&,3UREH$VVHPEO\ List of Illustrations )LJXUH$3&,SUREHDVVHPEO\ )LJXUH(6,SUREHDVVHPEO\ Finnigan: LCQ MS Detector Hardware Manual 4-i

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innigan: LCQ MS Detector Hardware Manual 4-1

121 CHANGING API PROBE ASSEMBLIES Removing the APCI Probe Assembly 4.1 Removing the APCI Probe Assembly 7RUHPRYHWKH$3&,SUREHDVVHPEO\SURFHHGDVIROORZV5HIHUWR )LJXUHIRUWKHORFDWLRQRIWKHFRPSRQHQWVRIWKH$3&,SUREH DVVHPEO\ 6WRSWKHIORZRIVDPSOHVROXWLRQIURPWKH/&RUV\ULQJH SXPSLQWRWKH$3&,SUREH 2. Place the electronics service switch in the Service (OFF, O) position. When you place the electronics service switch in the Service (OFF, O) position, the voltages to the API source (including the APCI probe), ion optics, mass analyzer, and ion detection system are turned off and the flows of sheath gas and auxiliary gas into the APCI probe are stopped. :$51,1*0DNHVXUHWKDWWKH/&4HOHFWURQLFVVHUYLFHVZLWFKLV LQWKH6HUYLFH2))2SRVLWLRQEHIRUH\RXSURFHHG :$5181*.RQWUROOLHUHQ6LHGD GHU+DXSWVFKDOWHUI U/&4 (OHNWURQLNWHLOHDXI:DUWXQJ6HUYLFH2))2JHVFKDOWHWLVWEHYRU 6LHIRUWIDKUHQ $9(57,66(0(176DVVXUHUTXHOHGLVMRQFWHXUGHEUDQFKHPHQW /&4HVWHQSRVLWLRQ6HUYLFH2))2DYDQWGHFRQWLQXHU 3. Loosen the two flange retainer bolts that secure the APCI probe assembly to the API spray shield. See Figure XOOEDFNWKH$3&,SUREHDVVHPEO\IURPWKHVSUD\VKLHOG 4-2 Finnigan: LCQ MS Detector Hardware Manual

122 CHANGING API PROBE ASSEMBLIES Removing the APCI Probe Assembly APCI FLANGE FLANGE RETAINER BOLT (2 ) VAPORIZER HEATER CABLE AUXILIARY GAS LINE FITTING SAMPLE TRANSFER LINE FITTING (RED) SPRAY SHIELD PROBE SLIDE ADAPTER FASTENER (UNDERSIDE) CORONA NEEDLE HIGH VOLTAGE CONNECTOR SHEATH GAS LINE FITTING (BLUE) Figure 4-1. APCI probe assembly 'LVFRQQHFWWKHFRURQDQHHGOHKLJKYROWDJHFDEOHIURPWKH FRURQDQHHGOHKLJKYROWDJHFRQQHFWRU7RGLVFRQQHFWWKHFDEOH WXUQWKHORFNLQJULQJRQWKHFDEOHFRXQWHUFORFNZLVHXQWLO\RX FDQSXOOWKHFDEOHIUHH 'LVFRQQHFWWKHYDSRUL]HUKHDWHUFDEOHIURPWKHFRQQHFWRURQ WKHIURQWSDQHO/HDYHWKHFDEOHFRQQHFWHGWRWKH$3&, IODQJH7RGLVFRQQHFWWKHFDEOHSXOOEDFNRQWKHORFNLQJULQJ RQWKHFDEOH 7. Disconnect the sample transfer line from the APCI probe by turning the sample transfer line fitting counterclockwise until you can pull the transfer line and fitting free from the probe. 8. Disconnect the sheath gas line from the APCI probe by turning the sheath gas line fitting counterclockwise until you can pull the sheath gas line and fitting free from the probe. Finnigan: LCQ MS Detector Hardware Manual 4-3

123 CHANGING API PROBE ASSEMBLIES Removing the APCI Probe Assembly 9. Disconnect the auxiliary gas line from the APCI probe by turning the auxiliary gas line fitting counterclockwise until you can pull the auxiliary gas line and fitting free from the probe. :$51,1*7KH$3&,YDSRUL]HUKHDWHUFDQUHDFKWHPSHUDWXUHV RI &$OZD\VDOORZWKH$3&,SUREHWRFRROWRDPELHQW WHPSHUDWXUHVEHIRUHKDQGOLQJRUUHPRYLQJWKH$3&,SUREHIURP WKH$3&,IODQJH :$5181*'HU$3&,9HUGDPSIHUNDQQ7HPSHUDWXUHQELV]X ƒ&huuhlfkhq/dvvhq6lhglh$3&,6rqghvwhwvdxi 8PJHEXQJVWHPSHUDWXUDEN KOHQEHYRU6LHVLHEHU KUHQRGHUDXV GHP$3&,)ODQVFKDXVEDXHQ $9(57,66(0(17/HYDSRULVDWHXU$3&,SHXWDWWHLQGUHXQH WHPSpUDWXUHGHƒ&/DLVVHUWRXMRXUVODVRQGH$3&,UHIURLGLUj WHPSpUDWXUHDPELDQWHDYDQWGHODVpSDUHUGHODEULGH$3&, 10. With one hand holding the APCI flange, loosen the knurled fastener that secures the APCI flange to the probe slide adapter. 11. Remove the APCI probe assembly from the probe slide adapter by sliding it off the probe slide adapter. Place the APCI probe assembly on a lint-free tissue and allow it to cool to ambient temperature (approximately 20 min). 12. Remove the corona discharge needle from the APCI probe assembly by pulling it free from the corona discharge needle assembly. Store the corona discharge needle by inserting it into one of the foam walls of the APCI probe assembly storage container. 13. Store the APCI probe assembly in its foam storage container. (Make sure that the APCI probe assembly is at ambient temperature before you place it in its storage container.) 4-4 Finnigan: LCQ MS Detector Hardware Manual

124 CHANGING API PROBE ASSEMBLIES Installing the ESI Probe Assembly 4.2 Installing the ESI Probe Assembly To install the ESI probe assembly, remove the APCI probe assembly using the procedure described in the previous section; then proceed as follows. Refer to Figure 4-2 for the location of the components of the ESI probe assembly. 1. Place the electronics service switch in the Service (OFF, O) position. :$51,1*0DNHVXUHWKDWWKH/&4HOHFWURQLFVVHUYLFHVZLWFKLV LQWKH6HUYLFH2))2SRVLWLRQEHIRUH\RXSURFHHG :$5181*.RQWUROOLHUHQ6LHGD GHU+DXSWVFKDOWHUI U/&4 (OHNWURQLNWHLOHDXI:DUWXQJ6HUYLFH2))2JHVFKDOWHWLVWEHYRU 6LHIRUWIDKUHQ $9(57,66(0(176DVVXUHUTXHOHGLVMRQFWHXUGHEUDQFKHPHQW /&4HVWHQSRVLWLRQ6HUYLFH2))2DYDQWGHFRQWLQXHU 1. Remove the ESI probe assembly from its storage container. 2. If your ESI probe assembly does not already contain a sample tube (fused-silica capillary), you need to follow the procedure for installing a sample tube that is outlined in the topic Maintaining the ESI Probe in the MS Detector Maintenance chapter. 1RWH(QVXUHWKDWWKHSUREHUHWDLQHUEROWLVWLJKWDQGWKH(6, SUREHLVVHFXUHGWRWKH(6,IODQJH FLANGE RETAINER BOLT Finnigan: LCQ MS Detector Hardware Manual 4-5

125 CHANGING API PROBE ASSEMBLIES Installing the ESI Probe Assembly ESI FLANGE SHEATH GAS LINE FITTING (BLUE) SAMPLE TRANSFER LINE FITTING FLANGE RETAINER BOLT PROBE SLIDE ADAPTER AUXILIARY GAS LINE FITTING SHEATH LIQUID INLET HIGH VOLTAGE CONNECTOR Figure 4-2. ESI probe assembly 4. Slide the ESI probe assembly onto the probe slide adapter. Secure the ESI probe assembly to the probe slide adapter with the knurled fastener that is located on the underside of the probe slide adapter. 5. With one hand, hold the ESI probe on the nozzle side of the probe to keep it from moving in the flange. With the other hand, connect the high voltage power cable to the connector labeled HV on the ESI probe. Turn the locking-ring on the cable clockwise to secure the cable. 6. Push the ESI probe assembly against the spray shield. 6HFXUHWKH(6,IODQJHWRWKHVSUD\VKLHOGZLWKWKHWZRIODQJH UHWDLQHUEROWV &RQQHFWWKHVKHDWKJDVOLQHDQGEOXHILWWLQJWRWKHLQOHW ODEHOHG6KHDWK*DVRQWKH(6,SUREH 4-6 Finnigan: LCQ MS Detector Hardware Manual

126 CHANGING API PROBE ASSEMBLIES Installing the ESI Probe Assembly &RQQHFWWKHDX[LOLDU\JDVOLQHDQGJUHHQILWWLQJWRWKHLQOHW ODEHOHG$X[*DVRQWKH(6,SUREH &RQQHFWWKHVDPSOHWUDQVIHUOLQHWRWKHJURXQGHGILWWLQJ,I\RXDUHXVLQJVKHDWKOLTXLGFRQQHFWWKHVKHDWKOLTXLGOLQH DQGILWWLQJWRWKHLQOHWODEHOHG6KHDWK/LTXLGRQWKH(6, SUREH,I\RXDUHQRWXVLQJVKHDWKOLTXLGHQVXUHWKDWWKH 7HI]HO SOXJILWWLQJ31LVLQVHUWHGLQ WKHVKHDWKOLTXLGLQOHWRQWKH(6,SUREH 3ODFHWKHHOHFWURQLFVVHUYLFHVZLWFKLQWKH1RUPDO21 SRVLWLRQ,QWKH7XQH3OXVZLQGRZFKRRVHSetup Change to ESIWR FRQILJXUHWKH/&4IRU(6,RSHUDWLRQ 1RWH7KH/&4GDWDV\VWHPFRQWDLQVFXVWRPL]HGWXQHILOHVLQWKH &?/&4?0HWKRGVGLUHFWRU\IRUDYDULHW\RIDSSOLFDWLRQVLQFOXGLQJ (6,KLJKIORZDQG(6,ORZIORZ/&06H[SHULPHQWV,QWKH7XQH 3OXVZLQGRZFKRRVHFile OpenWRGLVSOD\WKHWXQHILOHV5HIHUWR WKH/&42SHUDWRUªV0DQXDOIRULQVWUXFWLRQVRQWXQLQJDQG RSHUDWLQJWKH/&4LQWKH(6,PRGH Finnigan: LCQ MS Detector Hardware Manual 4-7

127 CHANGING API PROBE ASSEMBLIES Removing the ESI Probe Assembly 4.3 Removing the ESI Probe Assembly 7RUHPRYHWKH(6,SUREHDVVHPEO\SURFHHGDVIROORZV 5HIHUWR)LJXUHRQSDJHIRUWKHORFDWLRQRIWKH FRPSRQHQWVRIWKH(6,SUREHDVVHPEO\ 6WRSWKHIORZRIVDPSOHVROXWLRQIURPWKH/&RUV\ULQJH SXPSLQWRWKH(6,SUREH,IQHFHVVDU\VWRSWKHIORZRIVKHDWKOLTXLGLQWRWKH(6, VRXUFH 1. Place the electronics service switch in the Service (OFF, O) position. When you place the electronics service switch in the Service (OFF, O) position, the voltages to the API source (including the ESI probe), ion optics, mass analyzer, and ion detection system are turned off. The flows of sheath gas and auxiliary gas into the ESI probe are stopped. :$51,1*0DNHVXUHWKDWWKH/&4HOHFWURQLFVVHUYLFHVZLWFKLV LQWKH6HUYLFH2))2SRVLWLRQEHIRUH\RXSURFHHG :$5181*.RQWUROOLHUHQ6LHGD GHU+DXSWVFKDOWHUI U/&4 (OHNWURQLNWHLOHDXI:DUWXQJ6HUYLFH2))2JHVFKDOWHWLVWEHYRU 6LHIRUWIDKUHQ $9(57,66(0(176DVVXUHUTXHOHGLVMRQFWHXUGHEUDQFKHPHQW /&4HVWHQSRVLWLRQ6HUYLFH2))2DYDQWGHFRQWLQXHU 'LVFRQQHFWWKHKLJKYROWDJHFDEOHIURPWKHFRQQHFWRUODEHOHG +9RQWKH(6,SUREH7RGLVFRQQHFWWKHFDEOHWXUQWKH ORFNLQJULQJRQWKHFDEOHFRXQWHUFORFNZLVHXQWLO\RXFDQSXOO WKHFDEOHIUHH 4-8 Finnigan: LCQ MS Detector Hardware Manual

128 CHANGING API PROBE ASSEMBLIES Removing the ESI Probe Assembly 2. Disconnect the sample transfer line from the grounded transfer line fitting on the ESI flange. See Figure 4-2. (The sample transfer line is the line that comes from the LC, divert/injector valve, or syringe pump. It is not the fused silica capillary that enters the ESI probe.) 3. Disconnect the sheath gas line and fitting from the inlet labeled Sheath Gas on the ESI probe by turning the sheath gas line fitting (blue) counterclockwise until you can pull the sheath gas line and fitting free from the probe. 4. Disconnect the auxiliary gas line and fitting from the inlet labeled Aux Gas on the ESI probe by turning the auxiliary gas line fitting (green) counterclockwise until you can pull the auxiliary gas line and fitting free from the probe. 5. If the sheath liquid line is attached to the ESI probe, disconnect the sheath liquid line and fitting from the inlet labeled Sheath Liquid on the ESI probe by turning the sheath liquid line fitting counterclockwise until you can pull the sheath liquid line and fitting free from the probe. 6. Loosen the two flange retainer bolts that secure the ESI probe assembly to the spray shield. 7. Pull back the ESI probe assembly from the spray shield. 8. With one hand holding the ESI flange, loosen the knurled fastener that secures the ESI flange to the probe slide adapter. 9. Slide the ESI probe assembly off the probe slide adapter. Store the ESI probe assembly in its foam storage container. Finnigan: LCQ MS Detector Hardware Manual 4-9

129 CHANGING API PROBE ASSEMBLIES Installing the APCI Probe Assembly 4.4 Installing the APCI Probe Assembly To install the APCI probe assembly, remove the ESI probe assembly using the procedure in the previous section; then proceed as follows. Refer to Figure 4-1 on page 4-3 for the location of the components of the APCI probe assembly. 1. Place the electronics service switch in the Service (OFF, O) position. :$51,1*0DNHVXUHWKDWWKH/&4HOHFWURQLFVVHUYLFHVZLWFKLV LQWKH6HUYLFH2))2SRVLWLRQEHIRUH\RXSURFHHG :$5181*.RQWUROOLHUHQ6LHGD GHU+DXSWVFKDOWHUI U/&4 (OHNWURQLNWHLOHDXI:DUWXQJ6HUYLFH2))2JHVFKDOWHWLVWEHYRU 6LHIRUWIDKUHQ $9(57,66(0(176DVVXUHUTXHOHGLVMRQFWHXUGHEUDQFKHPHQW /&4HVWHQSRVLWLRQ6HUYLFH2))2DYDQWGHFRQWLQXHU 1. Remove the APCI probe assembly and corona discharge needle from the storage container. 2. Insert the corona discharge needle into its socket in the corona discharge needle assembly. 3. If your APCI probe assembly does not already contain a sample tube (fused-silica capillary), you need to follow the procedure for installing a sample tube that is outlined in the topic Maintaining the APCI Probe in the MS Detector Maintenance chapter. Ensure that the probe retainer bolt is tight and the APCI probe is secured to the APCI flange Finnigan: LCQ MS Detector Hardware Manual

130 CHANGING API PROBE ASSEMBLIES Installing the APCI Probe Assembly &DXWLRQ,QVSHFWWKH$3&,SUREH0DNHVXUHWKDWWKHJUHHQ JURXQGZLUHLVQRWWRXFKLQJWKHYDSRUL]HUFDVLQJ5HSRVLWLRQWKH JURXQGZLUHLIQHFHVVDU\ 5. Slide the APCI probe assembly onto the probe slide adapter. Secure the APCI probe assembly to the probe slide adapter with the knurled fastener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ush the APCI probe assembly against the spray shield. 6HFXUHWKH$3&,IODQJHWRWKHVSUD\VKLHOGZLWKWKHWZRIODQJH UHWDLQHUEROWV 3ODFHWKHHOHFWURQLFVVHUYLFHVZLWFKLQWKH1RUPDO21 SRVLWLRQ,QWKH7XQH3OXVZLQGRZFKRRVHSetup Change to APCIWR FRQILJXUHWKH/&4IRU$3&,RSHUDWLRQ 1RWH7KH/&4GDWDV\VWHPFRQWDLQVFXVWRPL]HGWXQHILOHVLQWKH &?/&4?0HWKRGVGLUHFWRU\IRUDYDULHW\RIDSSOLFDWLRQVLQFOXGLQJ $3&,KLJKIORZDQG$3&,ORZIORZ/&06H[SHULPHQWV,QWKH 7XQH3OXVZLQGRZFKRRVHFile OpenWRGLVSOD\WKHWXQHILOHV 5HIHUWRWKH/&42SHUDWRUªV0DQXDOIRULQVWUXFWLRQVRQWXQLQJ DQGRSHUDWLQJWKH/&4LQWKH$3&,PRGH Finnigan: LCQ MS Detector Hardware Manual 4-11

131 MS DETECTOR MAINTENANCE Contents Contents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innigan: LCQ MS Detector Hardware Manual 5-i

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133 MS DETECTOR MAINTENANCE Contents )LJXUH([SORGHGYLHZRIWKHHOHFWURQPXOWLSOLHUVKRZLQJWKHDQRGH )LJXUH([SORGHGYLHZRIWKHHOHFWURQPXOWLSOLHUVKRZLQJWKHFDWKRGH )LJXUH7XUERPROHFXODUSXPS List of Tables 7DEOH06'HWHFWRU0DLQWHQDQFH3URFHGXUHV 7DEOH7RROV(TXLSPHQWDQG&KHPLFDOV Finnigan: LCQ MS Detector Hardware Manual 5-iii

134 MS DETECTOR MAINTENANCE 5. MS DETECTOR MAINTENANCE /&4SHUIRUPDQFHGHSHQGVRQWKHPDLQWHQDQFHRIDOOSDUWVRIWKH LQVWUXPHQW,WLV\RXUUHVSRQVLELOLW\WRPDLQWDLQ\RXU V\VWHPSURSHUO\E\SHUIRUPLQJWKHV\VWHPPDLQWHQDQFH SURFHGXUHVRQDUHJXODUEDVLV 7KLVFKDSWHUGHVFULEHVURXWLQH06GHWHFWRUPDLQWHQDQFH SURFHGXUHVWKDWPXVWEHSHUIRUPHGWRHQVXUHRSWLPXP SHUIRUPDQFHRIWKHLQVWUXPHQW0RVWRIWKHSURFHGXUHVLQYROYH FOHDQLQJ)RUH[DPSOHSURFHGXUHVDUHSURYLGHGIRUFOHDQLQJWKH $3,VRXUFHLRQRSWLFVPDVVDQDO\]HUDQGLRQGHWHFWLRQV\VWHP 3URFHGXUHVDUHDOVRSUHVHQWHGIRUUHSODFLQJWKH$3,VDPSOHWXEH KHDWHGFDSLOODU\DQGHOHFWURQPXOWLSOLHU 5RXWLQHDQGLQIUHTXHQW06GHWHFWRUPDLQWHQDQFHSURFHGXUHVDUH OLVWHGLQ7DEOH Table 5-1. MS Detector Maintenance Procedures MS Detector Component Procedure Frequency Procedure Location API source API source Flush (clean) sample transfer line, sample tube, and API probe Flush (clean) heated capillary Daily Page 5-8 Daily (or more often*) Page 5-10 API source Flush (clean) spray shield Daily (or more often*) Page 5-10 Rotary-vane pump Purge (decontaminate) oil Daily Page 5-74 API source Clear heated capillary If heated capillary bore is obstructed API source Replace heated capillary If heated capillary bore is corroded or if heater fails Page 5-13 Page 5-33 Finnigan: LCQ MS Detector Hardware Manual 5-1

135 MS DETECTOR MAINTENANCE MS Detector Component Procedure Frequency Procedure Location API source Clean API stack (spray shield, heated capillary, tube lens, and skimmer) As needed* Page 5-33 API source Replace sample tube If sample tube is broken or obstructed Ion optics Clean octapoles and interoctapole lens As needed* Page 5-44 Mass analyzer Clean mass analyzer Yearly (or as needed*) Page 5-44 Ion detection system Ion detection system Turbomolecular pump Turbomolecular pump Clean ion detection system (electron multiplier and conversion dynode) Replace electron multiplier anode and cathode Whenever the top cover plate of the vacuum manifold is removed If noise in spectrum is excessive or proper electron multiplier gain can not be achieved Pages 5-16 (APCI) and 5-24 (ESI) Page 5-57 Page Change oil reservoir At least once a year Page Replace turbomolecular pump If turbomolecular pump fails Page Cooling fans Clean fan filter Every 4 months Page 5-80 Rotary-vane pump Add oil If oil level is low Manufacturer s documentation Rotary-vane pump Change oil Every 3 months or if oil is cloudy or discolored Manufacturer s documentation Fuses Replace fuse If fuse has blown Diagnostics and PCB and Assembly Replacement chapter Electronic modules Replace electronic module If electronic module fails Diagnostics and PCB and Assembly Replacement chapter PCBs Replace PCB If PCB fails Diagnostics and PCB and Assembly Replacement chapter Ion gauge Replace ion gauge If ion gauge fails Diagnostics and PCB and Assembly Replacement chapter Frequency depends on analytical conditions 5-2 Finnigan: LCQ MS Detector Hardware Manual

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137 MS DETECTOR MAINTENANCE Tools and Supplies 5.1 Tools and Supplies 7KH/&4UHTXLUHVYHU\IHZWRROVIRU\RXWRSHUIRUPURXWLQH PDLQWHQDQFHSURFHGXUHV<RXFDQUHPRYHDQGGLVDVVHPEOHPDQ\ RIWKHFRPSRQHQWVE\KDQG7KHWRROVHTXLSPHQWDQGFKHPLFDOV OLVWHGLQ7DEOHDUHQHHGHGIRUWKHPDLQWHQDQFHRIWKH$3, VRXUFHLRQRSWLFVPDVVDQDO\]HUDQGLRQGHWHFWLRQV\VWHP Table 5-2. Tools, Equipment, and Chemicals Description Wrench, 5/16-in., hex socket (Allen) Wrench, 9/16-in., socket Wrench, 7/16-in., open end Wrench, 9/16-in., open end Wrench, 5/16-in., open end Wrench, 1/2-in., open end Wrench, 3/8-in., open end Screwdrivers, set, ball point, Allen (also referred to as ball drivers) Screwdriver, slot head, large Screwdriver, slot head, small Screwdriver, Phillips, small Fused-silica cutting tool Part Number Hypodermic tube Spray bottle Beaker, 450 ml Gloves, nylon Kimwipes or other lint-free industrial tissue Applicators (swabs), cotton-tipped Detergent Clean, dry, compressed nitrogen gas Distilled water Methanol, HPLC grade or better 5-4 Finnigan: LCQ MS Detector Hardware Manual

138 MS DETECTOR MAINTENANCE Tools and Supplies :$51,1*$VZLWKDOOFKHPLFDOVVROYHQWVDQGUHDJHQWVVKRXOG EHVWRUHGDQGKDQGOHGDFFRUGLQJWRVWDQGDUGVDIHW\SURFHGXUHVDQG VKRXOGEHGLVSRVHGRIDFFRUGLQJWRORFDODQGIHGHUDOUHJXODWLRQV :$5181*:LHDOOH&KHPLNDOLHQVLQG/ VXQJVPLWWHOXQG 5HDJHQ]LHQHQWVSUHFKHQGGHQ EOLFKHQ6LFKHUKHLWVEHVWLPPXQJHQ]X ODJHUQXQGKDQG]XKDEHQVRZLHJHPl ORNDOHUXQG EHUJHRUGQHWHU %HVWLPPXQJHQ]XHQWVRUJHQ $9(57,66(0(17$LQVLTXHSRXUWRXWSURGXLWFKLPLTXHOHV VROYDQWVHWUpDFWLIVGRLYHQWrWUHFRQVHUYpVHWPDQLSXOpVHQ UHVSHFWDQWWRXWHVOHVSURFpGXUHVXVXHOOHVGHVpFXULWpHWGRLYHQW rwuhplvdx[gpfkhwvfrqiruppphqwdx[uqjohphqwvorfdx[hw QDWLRQDX[ Finnigan: LCQ MS Detector Hardware Manual 5-5

139 MS DETECTOR MAINTENANCE Frequency of Cleaning 5.2 Frequency of Cleaning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innigan: LCQ MS Detector Hardware Manual

140 MS DETECTOR MAINTENANCE API Source Maintenance 5.3 API Source Maintenance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b+5/,&+(168%67$1=(19(50(,'(1%hlghu 9HUZHQGXQJYRQ/ VXQJVRGHU.RUURVLRQVPLWWHOQVLQGVWHWV 6FKXW]KDQGVFKXKHXQG6FKXW]EULOOH]XWUDJHQ$EOX VWRIIHVLQG DXI]XIDQJHQXQGJXWH%HO IWXQJPXVVYRUKDQGHQVHLQ1lKHUH,QIRUPDWLRQ EHUGHQ8PJDQJPLWEHVWLPPWHQ/ VXQJVPLWWHOQ N QQHQ6LHGHQ6LFKHUKHLWVGDWHQEOlWWHUQYRP/LHIHUDQWHQGHU MHZHLOLJHQ6XEVWDQ]HQWQHKPHQ Finnigan: LCQ MS Detector Hardware Manual 5-7

141 MS DETECTOR MAINTENANCE API Source Maintenance $9(57,66(0(17(9,7(5'(75((;326($8; 352'8,76327(17,(//(0(17'$1*(5(8;3RUWHU WRXMRXUVGHVJDQWVGHSURWHFWLRQHWGHVOXQHWWHVGHVpFXULWpSRXU XWLOLVHUGHVVROYDQWVRXGHVPDWLqUHVFRUURVLYHV(QRXWUH UHQIHUPHUOHVpFRXOHPHQWVGHGpFKHWVHWPDLQWHQLUXQHYHQWLODWLRQ DSSURSULpH&RQVXOWHUOHVILFKHVWR[LFRORJLTXHV06'6GX IRXUQLVVHXUSRXUOHPRGHGHPSORLGXQVROYDQWSDUWLFXOLHU Flushing the Sample Transfer Line, Sample Tube, and API Probe <RXVKRXOGIOXVKWKHVDPSOHWUDQVIHUOLQHVDPSOHWXEHDQG$3, SUREHDWWKHHQGRIHDFKZRUNLQJGD\RUPRUHRIWHQLI\RXVXVSHFW WKH\DUHFRQWDPLQDWHGE\IORZLQJDPHWKDQROGLVWLOOHG ZDWHUVROXWLRQIURPWKH/&WKURXJKWKH$3,VRXUFH 7RIOXVKWKHVDPSOHWUDQVIHUOLQHVDPSOHWXEHDQG$3,SUREH SURFHHGDVIROORZV Make sure that the API flange is secured to the spray shield by the two flange retainer bolts. From the Tune Plus window, choose Control On (or click on the On/Standby button) to turn on the voltages and gas flows to the API source.,i\rxduhrshudwlqjlq$3&,prghjrwrvwhs,i\rxduhrshudwlqjlq(6,prghjrwrvwhs Set up the APCI source as follows: a. In the Tune Plus window, choose Setup APCI Source (or click on the APCI Source button). The APCI Source dialog box appears. b. In the APCI Source dialog box, use the Vaporizer Temp spin box to set the vaporizer temperature to 500 C. c. Use the Sheath Gas Flow Rate spin box to set the sheath gas flow rate to Finnigan: LCQ MS Detector Hardware Manual

142 MS DETECTOR MAINTENANCE API Source Maintenance d. Use the Discharge Current spin box to set the discharge current to 0. e. Use the Aux Gas Flow Rate spin box to set the auxiliary gas flow rate to 10. f. Click on OK to set the APCI parameters and close the dialog box. Go to step 5. Set up the ESI source as follows: a. In the Tune Plus window, choose Setup ESI Source (or click on the ESI Source button). The ESI Source dialog box appears. b. Use the Spray Voltage spin box to set the spray voltage to 0. c. Use the Sheath Gas Flow Rate spin box to set the sheath gas flow rate to 80. d. Use the Aux Gas Flow Rate spin box to set the auxiliary gas flow rate to 10. e. Click on OK to set the ESI parameters and close the dialog box. Set up and start a flow of 50:50 methanol:water solution from the LC to the API source, as follows: a. In the Tune Plus window, choose Setup LC Pump (or click on the LC Pump button). The LC Pump dialog box appears. b. In the LC Pump dialog box, use the Flow Rate spin box to set the flow rate to a value that is typical for your experiments. c. Use the Solvent spin boxes to set the flow to 50% methanol and 50% distilled water. d. Select the 2Q RSWLRQEXWWRQ. Then click on OK to start the LC pump. Finnigan: LCQ MS Detector Hardware Manual 5-9

143 MS DETECTOR MAINTENANCE API Source Maintenance Let the solution flow through the sample transfer line, sample tube, and API probe for 15 min. After 15 min, turn off the flow of liquid from the LC to the API source, as follows. Leave the API source (including the APCI vaporizer, sheath gas, and auxiliary gas) on for an additional 5 min. a. In the Tune Plus window, choose Setup LC Pump (or click on the LC button). The LC Pump dialog box appears. b. Select the 2II RSWLRQEXWWRQ. Then click on OK to stop the LC pump. After 5 min, turn off the API source by placing the MS detector in Standby: From the Tune Plus window, choose Control Standby (or click on the On/Standby button) to put the MS detector in Standby. Go on to the next topic: Cleaning the Spray Shield and the Heated Capillary Flushing the Spray Shield and Heated Capillary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urn off the flow of liquid from the LC (or other sample introduction device) to the API source. To turn off the flow of liquid from the LC to the API source, do the following: a. In the Tune Plus window, choose Setup LC Pump (or click on the LC button). The LC Pump dialog box appears Finnigan: LCQ MS Detector Hardware Manual

144 MS DETECTOR MAINTENANCE API Source Maintenance b. Select the 2II option button and then click on OK to stop the LC pump. From the Tune Plus window, choose Control Standby (or click on the On/Standby button) to put the MS detector in Standby. :$51,1*3ODFHWKH06GHWHFWRULQ6WDQGE\RU2IIEHIRUH\RX RSHQWKHDWPRVSKHULFSUHVVXUHLRQL]DWLRQ$3,VRXUFH7KH SUHVHQFHRIDWPRVSKHULFR[\JHQLQWKH$3,VRXUFHZKHQWKH06 GHWHFWRULV2QFRXOGEHXQVDIH/&4DXWRPDWLFDOO\WXUQVWKH06 GHWHFWRU2IIZKHQ\RXRSHQWKH$3,VRXUFHKRZHYHULWLVEHVWWR WDNHWKLVDGGHGSUHFDXWLRQ :$5181*6WHOOHQ6LHGHQ06'HWHNWRUDXI%HUHLWVFKDIWRGHU $86EHYRU6LHGLH$WPRVSKlUHQGUXFN,RQLVDWLRQVTXHOOH$3, IIQHQ/XIWVDXHUVWRIILQGHU$3,4XHOOHNDQQEHLHLQJHVFKDOWHWHP 06'HWHNWRUHLQH*HIDKUGDUVWHOOHQ'DV/&4VFKDOWHWGHQ06 'HWHNWRUDXWRPDWLVFK$86ZHQQ6LHGLH$3,4XHOOH IIQHQ(V ZLUGMHGRFKHPSIRKOHQGLHVH]XVlW]OLFKH9RUVLFKWVPD QDKPH]X HUJUHLIHQ $9(57,66(0(170HWWH]OHGpWHFWHXUGH60HQYHLOOHXVHRX KRUVWHQVLRQDYDQWGªRXYULUODVRXUFHGªLRQLVDWLRQjODSUHVVLRQ DWPRVSKpULTXH7RXWHSUpVHQFHGªR[\JqQHGDQVODVRXUFH GªLRQLVDWLRQORUVTXHOHGpWHFWHXUGH60HVWDFWLYpSHXWFRPSRUWHU GHVULVTXHV%LHQTXH/&4GpVDFWLYHDXWRPDWLTXHPHQWOH GpWHFWHXUGH60ORUVTXHYRXVRXYUH]ODVRXUFHGªLRQLVDWLRQjOD SUHVVLRQDWPRVSKpULTXHLOHVWSUpIpUDEOHGHSUHQGUHFHWWH SUpFDXWLRQVXSSOpPHQWDLUH Loosen the two flange retainer bolts that secure the API flange (APCI or ESI flange) to the spray shield. Finnigan: LCQ MS Detector Hardware Manual 5-11

145 MS DETECTOR MAINTENANCE API Source Maintenance Pull back the API flange from the spray shield. :$51,1*$92,'%8516$WRSHUDWLQJWHPSHUDWXUHVWKH $3&,YDSRUL]HUDQGKHDWHGFDSLOODU\FDQVHYHUHO\EXUQ\RX 7KH$3&,YDSRUL]HUW\SLFDOO\RSHUDWHVDWWR &DQGWKH KHDWHGFDSLOODU\W\SLFDOO\RSHUDWHVDWWR &$OORZWKH KHDWHGYDSRUL]HUDQGKHDWHGFDSLOODU\WRFRROWRURRPWHPSHUDWXUH IRUDSSUR[LPDWHO\PLQEHIRUH\RXWRXFKRUUHPRYHHLWKHU FRPSRQHQW :$5181*9(5%5(1181*(19(50(,'(1'HU$3&, 9HUGDPSIHUXQGGLHEHKHL]WHQ.DSLOODUHQN QQHQVFKZHUH 9HUEUHQQXQJHQYHUXUVDFKHQ'LHQRUPDOH%HWULHEVWHPSHUDWXU GHV$3&,9HUGDPSIHUVOLHJW]ZLVFKHQXQGƒ&GLHGHU.DSLOODUHQ]ZLVFKHQXQGƒ&/DVVHQ6LHGHQ$3&, 9HUGDPSIHUXQGGLHEHKHL]WHQ.DSLOODUHQ0LQXWHQODQJDXI 8PJHEXQJVWHPSHUDWXUDEN KOHQEHYRU6LHHLQHGLHVHU.RPSRQHQWHQEHU KUHQRGHUHQWIHUQHQ $9(57,66(0(17(9,7(5/(6%58/85(6$ WHPSpUDWXUHGHIRQFWLRQQHPHQWOHYDSRULVDWHXU$3&,HWOH FDSLOODLUHFKDXIIpSHXYHQWLQIOLJHUGHVEU OXUHVVpYqUHV/H YDSRULVDWHXU$3&,IRQFWLRQQHQRUPDOHPHQWjGHVWHPSpUDWXUHVGH j R &HWOHFDSLOODLUHjGHVWHPSpUDWXUHVGHj R & /DLVVHUOHYDSRULVDWHXU$3&,HWOHFDSLOODLUHFKDXIIpUHIURLGLUj WHPSpUDWXUHDPELDQWHSHQGDQWHQYLURQPLQXWHVDYDQWG\ WRXFKHURXGHWHQWHUGHOHVGpSODFHU 7HPSRUDULO\SODFHDODUJH.LPZLSHRURWKHUOLQWIUHHWLVVXH RQWKHERWWRPRIWKHVSUD\VKLHOG7KH.LPZLSHLVUHTXLUHG WRDEVRUEWKHVROXWLRQXVHGWRIOXVKWKHKHDWHGFDSLOODU\DQG VSUD\VKLHOG )LOODVSUD\ERWWOHZLWKDVROXWLRQRI+3/&JUDGH PHWKDQROGLVWLOOHGZDWHU6SUD\DSSUR[LPDWHO\P/RIWKH VROXWLRQDWWKHRSHQLQJRIWKHKHDWHGFDSLOODU\'RQRWWRXFK WKHKHDWHGFDSLOODU\ZLWKWKHWLSRIWKHVSUD\ERWWOH 5-12 Finnigan: LCQ MS Detector Hardware Manual

146 MS DETECTOR MAINTENANCE API Source Maintenance 8VHWKHVSUD\ERWWOHILOOHGZLWKWKHVROXWLRQRI+3/& JUDGHPHWKDQROGLVWLOOHGZDWHUWRIOXVKFRQWDPLQDQWVIURPWKH LQWHULRUVXUIDFHRIWKHVSUD\VKLHOG 5HPRYHWKH.LPZLSH\RXXVHGWRDEVRUEWKHVROXWLRQ6ZDE WKHVXUIDFHRIWKHVSUD\VKLHOGZLWKDGU\.LPZLSH Ensure that you have removed any salt or other contaminants that may have been deposited on the spray shield. If you are operating in the ESI mode, wipe off the ESI nozzle with a Kimwipe soaked with the methanol:water solution. 1RWH,I\RXDUHILQLVKHGRSHUDWLQJ\RXU/&4IRUWKHGD\FDSWKH KHDWHGFDSLOODU\ZLWKWKHVHSWXP/HDYHWKH$3,IODQJH ZLWKGUDZQIURPWKHVSUD\VKLHOG3XUJHWKHRLOLQWKHURWDU\YDQH SXPSDVGHVFULEHGLQWKHWRSLF3XUJLQJWKH2LOLQWKH5RWDU\ 9DQH3XPSRQSDJH Clearing the Bore of the Heated Capillary 7KHERUHRIWKHKHDWHGFDSLOODU\FDQEHFRPHEORFNHGE\EXIIHUVDOWV RUKLJKFRQFHQWUDWLRQVRIVDPSOH$VWDLQOHVVVWHHOK\SRGHUPLF WXEHKDVEHHQLQFOXGHGLQ\RXUDFFHVVRU\NLWIRUFOHDULQJDEORFNHG KHDWHGFDSLOODU\,IWKHSUHVVXUHLQWKHFDSLOODU\VNLPPHUUHJLRQDVPHDVXUHGE\ WKH&RQYHFWURQJDXJHGURSVFRQVLGHUDEO\EHORZ7RUU\RX VKRXOGVXVSHFWDEORFNHGKHDWHGFDSLOODU\<RXFDQFKHFNWKH &RQYHFWURQJDXJHSUHVVXUHLQWKH9DFXXPGLDORJER[E\FKRRVLQJ Setup VacuumIURPWKH7XQH3OXVZLQGRZ <RXGRQRWKDYHWRYHQWWKHV\VWHPWRFOHDUWKHERUHRIWKHKHDWHG FDSLOODU\7RFOHDUWKHERUHRIWKHKHDWHGFDSLOODU\GRWKH IROORZLQJ 1. Turn off the flow of liquid from the LC (or other sample introduction device) to the API source. To turn off the flow of liquid from the LC to the API source, do the following: a. In the Tune Plus window, choose Setup LC Pump (or click on the LC button). The LC Pump dialog box appears. Finnigan: LCQ MS Detector Hardware Manual 5-13

147 MS DETECTOR MAINTENANCE API Source Maintenance b. Select the 2II RSWLRQEXWWRQ. Then click on OK to stop the LC pump. 3ODFHWKHHOHFWURQLFVVHUYLFHVZLWFKORFDWHGRQWKHULJKWVLGH RIWKH06GHWHFWRULQWKH6HUYLFH2))2SRVLWLRQ WRWXUQRII WKHQRQYDFXXPV\VWHPYROWDJHV :$51,1*0DNHVXUHWKDWWKH/&4HOHFWURQLFVVHUYLFHVZLWFKLV LQWKH6HUYLFH2))2SRVLWLRQEHIRUH\RXSURFHHG :$5181*.RQWUROOLHUHQ6LHGD GHU+DXSWVFKDOWHUI U/&4 (OHNWURQLNWHLOHDXI:DUWXQJ6HUYLFH2))2JHVFKDOWHWLVWEHYRU 6LHIRUWIDKUHQ $9(57,66(0(176DVVXUHUTXHOHGLVMRQFWHXUGHEUDQFKHPHQW /&4HVWHQSRVLWLRQ6HUYLFH2))2DYDQWGHFRQWLQXHU 3. Loosen the two flange retainer bolts that secure the API flange (APCI or ESI) to the spray shield. 4. Pull back the API flange from the spray shield. :$51,1*7KH$3&,YDSRUL]HUW\SLFDOO\RSHUDWHVDW WR &DQGWKHKHDWHGFDSLOODU\W\SLFDOO\RSHUDWHVDW WR & 5-14 Finnigan: LCQ MS Detector Hardware Manual

148 MS DETECTOR MAINTENANCE API Source Maintenance :$5181*'LHQRUPDOH%HWULHEVWHPSHUDWXUGHV$3&, 9HUGDPSIHUVOLHJW]ZLVFKHQXQGƒ&GLHGHU.DSLOODUHQ ]ZLVFKHQXQGƒ& $9(57,66(0(17/HYDSRULVDWHXU$3&,IRQFWLRQQH QRUPDOHPHQWjGHVWHPSpUDWXUHVGHjƒ&HWOHFDSLOODLUHj GHVWHPSpUDWXUHVGHjƒ& 5. Clear the bore of the heated capillary by inserting and withdrawing the 28 gauge, 10-in hypodermic tube (P/N ) included in your accessory kit. 6. Fill a spray bottle with a 50:50 solution of HPLC-grade methanol:distilled water. From a distance of 10 cm from the entrance end of the heated capillary, spray a small amount of the solution down the bore of the heated capillary. 7. Repeat steps 5 and 6 several times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innigan: LCQ MS Detector Hardware Manual 5-15

149 MS DETECTOR MAINTENANCE API Source Maintenance Maintaining the APCI Probe 7KH$3&,SUREHUHTXLUHVDPLQLPXPRIPDLQWHQDQFH7KH$3&, VDPSOHWXEHµ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innigan: LCQ MS Detector Hardware Manual

150 MS DETECTOR MAINTENANCE API Source Maintenance Figure 5-1. Cross sectional view of the APCI probe Finnigan: LCQ MS Detector Hardware Manual 5-17

151 MS DETECTOR MAINTENANCE API Source Maintenance Removing the APCI Probe Assembly,IWKH$3&,SUREHDVVHPEO\LVLQVWDOOHGRQWKH/&4ILUVWUHPRYH WKH$3&,SUREHDVVHPEO\DVGHVFULEHGLQWKHWRSLF5HPRYLQJWKH $3&,3UREH$VVHPEO\LQWKH&KDQJLQJ$3,3UREH $VVHPEOLHVFKDSWHU3ODFHWKH$3&,SUREHDVVHPEO\RQDOLQW IUHHWLVVXH :$51,1*$92,'%85167KH$3&,YDSRUL]HUKHDWHUFDQ UHDFKWHPSHUDWXUHVRI &$OZD\VDOORZWKH$3&,SUREHWRFRRO WRDPELHQWWHPSHUDWXUHVEHIRUHKDQGOLQJRUUHPRYLQJWKH$3&, SUREHIURPWKH$3&,IODQJH :$5181*9(5%5(1181*(19(50(,'(1'HU$3&, 9HUGDPSIHUNDQQ7HPSHUDWXUHQELV]Xƒ&HUUHLFKHQ/DVVHQ 6LHGLH$3&,6RQGHVWHWVDXI8PJHEXQJVWHPSHUDWXUDEN KOHQ EHYRU6LHVLHEHU KUHQRGHUDXVGHP$3&,)ODQVFKDXVEDXHQ $9(57,66(0(17(9,7(5/(6%58/85(6/H YDSRULVDWHXU$3&,SHXWDWWHLQGUHXQHWHPSpUDWXUHGHƒ& /DLVVHUWRXMRXUVODVRQGH$3&,UHIURLGLUjWHPSpUDWXUHDPELDQWH DYDQWGHODVpSDUHUGHODEULGH$3&, &DXWLRQ:UHQFKIODWVRQWKH$3&,SUREHFRPSRQHQWVDUH SURYLGHGIRU\RXUFRQYHQLHQFHZKHQ\RXGLVPDQWOHWKH$3&, SUREH1(9(586(7+(:5(1&+)/$76727,*+7(1 7+($3&,352%(&20321(1762QO\WLJKWHQWKH$3&, SUREHFRPSRQHQWVE\KDQG *RRQWRWKHQH[WWRSLF'LVDVVHPEOLQJWKH$3&,3UREH $VVHPEO\ 5-18 Finnigan: LCQ MS Detector Hardware Manual

152 MS DETECTOR MAINTENANCE API Source Maintenance Disassembling the APCI Probe Assembly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innigan: LCQ MS Detector Hardware Manual 5-19

153 MS DETECTOR MAINTENANCE API Source Maintenance Cleaning the APCI Probe Components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innigan: LCQ MS Detector Hardware Manual

154 MS DETECTOR MAINTENANCE API Source Maintenance,I\RXZDQWWRUHSODFHWKH$3&,VDPSOHWXEHJRRQWRWKHQH[W WRSLF5HPRYLQJWKH$3&,6DPSOH7XEH Removing the APCI Sample Tube 7RUHPRYHWKH$3&,VDPSOHWXEHIURPWKH$3&,PDQLIROGGRWKH IROORZLQJ :LWKDLQRSHQHQGZUHQFKUHPRYHWKHVDPSOHWXEHLQOHW ILWWLQJ31LQ,'2ULQJ31 DQGVDPSOHWXEHIURPWKH$3&,PDQLIROG6HH)LJXUH 5HPRYHWKHH[LWHQGQXW31LQ,'3((.IHUUXOH31DQGVDPSOHWXEH IURPWKHVDPSOHWXEHLQOHWILWWLQJ 'LVFDUGWKHROGVDPSOHWXEH *RRQWRWKHQH[WWRSLF,QVWDOOLQJWKH$3&,6DPSOH7XEH Installing the APCI Sample Tube 7RLQVWDOODQHZ$3&,VDPSOHWXEHSURFHHGDVIROORZV 8VHDIXVHGVLOLFDFXWWLQJWRROWRFXWDSLHFHRIµP,' µp2'ixvhgvlolfdwxelqj31wrdohqjwk RIDSSUR[LPDWHO\FPLQ(QVXUHWKDW\RXVTXDUHO\FXW WKHHQGVRIWKHIXVHGVLOLFDWXELQJ 2. Slide the exit-end nut (P/N ) and ferrule (P/N ) onto the length of the fused-silica tubing. See Figure Check the condition of the in. ID O-ring (P/N ) on the sample tube inlet fitting. Replace it if necessary. 4. Insert the fused-silica tubing into the sample tube inlet fitting. 5. Slide the exit-end nut and ferrule down the fused-silica tubing and into the sample tube inlet fitting. 6. Tighten the exit-end nut to secure the new sample tube (fusedsilica tubing). Finnigan: LCQ MS Detector Hardware Manual 5-21

155 MS DETECTOR MAINTENANCE API Source Maintenance IN. O-RING SAMPLE TUBE INLET FITTING FERRULE EXIT-END NUT SAMPLE TUBE (FUSED-SILICA TUBING) Figure 5-2. APCI sample tube connection 7. Gently slide the sample tube through the sample inlet of the APCI manifold. With a 3/8-in. open-end wrench, tighten down the sample tube inlet fitting to secure the fitting and compress the O- ring. 8. Unscrew and remove the vaporizer casing from the vaporizer flange (to expose the nozzle). 9. Gently slide the sample tube through the sheath gas tube of the APCI probe and out the APCI nozzle. Watch for the sample tube to exit the APCI nozzle. Screw the APCI manifold into the APCI probe (sheath gas tube). 10. Use a fused-silica cutting tool to cut the exit end of the sample tube so that approximately 1 mm protrudes past the tip of the APCI nozzle. 1RWH2QFHWKH$3&,VDPSOHWXEHKDVEHHQFXWWRWKHSURSHU OHQJWK\RXFDQUHPRYHWKH$3&,PDQLIROGDQGDFFXUDWHO\PHDVXUH DQGUHFRUGKRZIDUWKHVDPSOHWXEHH[WHQGVSDVWWKHHQGRIWKH $3&,PDQLIROG7KHOHQJWKVKRXOGEHDERXWFPLQ,Q WKHIXWXUHVDPSOHWXEHUHSODFHPHQWGRHVQRWUHTXLUHFRPSOHWH GLVDVVHPEO\RIWKH$3&,SUREHMXVWLQVWDOOWKHIXVHGVLOLFDWXELQJ LQWKH$3&,PDQLIROGDQGFXWLWWRWKHSURSHUOHQJWK Go on to the next topic: Reassembling the APCI Probe Assembly Reassembling the APCI Probe Assembly 7RUHDVVHPEOHWKH$3&,SUREHDVVHPEO\SURFHHGDVIROORZV 5-22 Finnigan: LCQ MS Detector Hardware Manual

156 MS DETECTOR MAINTENANCE API Source Maintenance 1. Unscrew and remove the APCI manifold from the APCI probe. Be careful not to damage the sample tube. 2. Gently screw the vaporizer casing back into the vaporizer flange. 3. Reinstall the heater coil and quartz insulator into the vaporizer casing. 4. Use a Phillips screwdriver to reattach the electrical ground wire to the vaporizer flange. Make sure that the flat side of the connector is against the vaporizer flange. 5. Reinstall the heater retainer and secure it with the socket-head screw. 6. Reinstall the APCI probe (minus the APCI manifold) into the APCI flange. 7. Carefully slide the sample tube through the APCI flange, through the sheath gas tube, and out the APCI nozzle. 8. With one hand holding the vaporizer casing to keep the probe from turning, screw the APCI manifold onto the APCI probe. 9. Rotate the APCI probe until the half-moon of the heater retainer is oriented away from the tip of the corona discharge needle (when the corona discharge needle is installed). Tighten the probe retainer bolt to secure the APCI probe to the APCI flange. 10. Move the ground wire away from the vaporizer casing. 11. Reinstall the corona discharge needle by inserting it into the socket in the corona discharge needle assembly. *RRQWRWKHQH[WWRSLF5HVLQVWDOOLQJWKH$3&,3UREH $VVHPEO\ Reinstalling the APCI Probe Assembly 7RUHLQVWDOOWKH$3&,SUREHDVVHPEO\RQWRWKH/&4IROORZWKH SURFHGXUHGHVFULEHGLQWKHWRSLF,QVWDOOLQJWKH$3&,3UREH $VVHPEO\LQWKH&KDQJLQJ$3,3UREH$VVHPEOLHVFKDSWHURU SODFHWKH$3&,SUREHDVVHPEO\LQLWVVWRUDJHFRQWDLQHU Finnigan: LCQ MS Detector Hardware Manual 5-23

157 MS DETECTOR MAINTENANCE API Source Maintenance Maintaining the ESI Probe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innigan: LCQ MS Detector Hardware Manual

158 MS DETECTOR MAINTENANCE API Source Maintenance 1RWH,I\RXRQO\ZDQWWRUHSODFHWKH(6,VDPSOHWXEHIROORZWKH SURFHGXUHVGHVFULEHGLQWKHWRSLFV5HPRYLQJWKH(6,3UREH $VVHPEO\'LVDVVHPEOLQJWKH(6,3UREH$VVHPEO\ 5HDVVHPEOLQJWKH(6,3UREH$VVHPEO\DQG5HLQVWDOOLQJWKH (6,3UREH$VVHPEO\ Removing the ESI Probe Assembly,IWKH(6,SUREHDVVHPEO\LVLQVWDOOHGRQWKH/&4UHPRYHWKH(6, SUREHDVVHPEO\DVGHVFULEHGLQWKHWRSLF5HPRYLQJWKH(6, 3UREH$VVHPEO\LQWKH&KDQJLQJ$3,3UREH$VVHPEOLHV FKDSWHU3ODFHWKH(6,SUREHDVVHPEO\RQDOLQWIUHHWLVVXH Disassembling the ESI Probe Assembly 7RGLVDVVHPEOHWKH(6,SUREHDVVHPEO\SURFHHGDVIROORZV 5HPRYHWKHWUDQVIHUOLQHILWWLQJIURPWKHJURXQGHGILWWLQJ KROGHURQWKH(6,IODQJH6HH)LJXUH 5HPRYHWKHVDPSOHWXEHDQGVDPSOHLQOHWILWWLQJIURPWKH(6, SUREHDVIROORZV D E 8QVFUHZWKHVDPSOHLQOHWILWWLQJ 5HPRYHWKHVDPSOHWXEHDQGVDPSOHLQOHWILWWLQJ /RRVHQWKHSUREHUHWDLQHUEROWWKDWKROGVWKH(6,SUREHLQWKH (6,IODQJHDQGUHPRYHWKH(6,SUREH3XOOWKH(6,SUREH WRZDUGWKHLQVLGHVSUD\FKDPEHUVLGHRIWKH(6,IODQJH Finnigan: LCQ MS Detector Hardware Manual 5-25

159 MS DETECTOR MAINTENANCE API Source Maintenance Figure 5-3. Cross sectional view of the ESI probe assembly Removing the ESI Nozzle, Needle, and Needle Seal <RXQHHGWRUHSODFHWKH(6,QHHGOHLILWLVGDPDJHG<RXQHHGWR UHSODFHWKHQHHGOHVHDOLIWKHVKHDWKOLTXLGLVOHDNLQJDWWKHQHHGOH VHDOQHHGOHLQWHUIDFH6HH)LJXUH 7RUHPRYHWKH(6,QR]]OHQHHGOHDQGQHHGOHVHDOSURFHHGDV IROORZV 1. Use a 5/16-in. wrench to loosen and remove the ESI nozzle from the ESI manifold. 2. Remove the ESI needle and needle seal from the ESI manifold. (If necessary, after you remove the needle, use the needle to push the needle seal out of the ESI manifold.) 3. If necessary, replace the needle seal (P/N ) and/or the ESI needle (P/N ) Finnigan: LCQ MS Detector Hardware Manual

160 MS DETECTOR MAINTENANCE API Source Maintenance Figure 5-4. Exploded view of the ESI probe Finnigan: LCQ MS Detector Hardware Manual 5-27

161 MS DETECTOR MAINTENANCE API Source Maintenance Cleaning the Manifold Sleeve and Ground Shield /LTXLGVFDQOHDNEHWZHHQWKHPDQLIROGVOHHYHDQGWKHKLJKYROWDJH JURXQGVKLHOGDQGFDXVHHOHFWULFDOVKRUWLQJ7KLVOHDNDJHXVXDOO\ UHVXOWVIURP/&IORZHQWHULQJWKH(6,VRXUFHZLWKRXWDVKHDWK JDV7KH/&IORZOHDNVEDFNWKURXJKWKHVKHDWKOLTXLGSRUW,I VDPSOHOLTXLGKDVOHDNHGLQWRWKHPDQLIROGVOHHYHJURXQGVKLHOG LQWHUIDFH\RXQHHGWRUHPRYHWKHJURXQGVKLHOGDQGFOHDQDQGGU\ WKHPDQLIROGVOHHYHDQGJURXQGVKLHOG 7RUHPRYHWKHJURXQGVKLHOGDQGWRFOHDQDQGGU\WKHPDQLIROG VOHHYHDQGJURXQGVKLHOGSURFHHGDVIROORZVVHH)LJXUH 1. Use a 1/2-in. wrench to remove the high voltage connector, star washer, and spring. 2. Pull the ground shield away from the ESI manifold and manifold sleeve. 3. Rinse the LC leakage away from the manifold sleeve and ground shield with distilled water and then rinse with methanol. 4. Use a Kimwipe to remove the excess methanol from the manifold sleeve and ground shield. 5. Dry the manifold sleeve and ground shield with nitrogen gas. 6. Reinstall the ground shield over the ESI manifold and manifold sleeve. Ensure that the in. ID O-ring (P/N ) is properly positioned between the manifold sleeve and ground shield. 7. Replace and tighten the high voltage connector (along with the star washer and spring) with a 1/2-in. wrench Cleaning the ESI Nozzle,IQHFHVVDU\FOHDQWKHERUHRIWKH(6,QR]]OHZLWKDQDSSURSULDWH VROYHQW7KLVZLOOGHSHQGRQWKHVROXELOLW\RIWKHFKHPLFDO GHSRVLWV7KHQULQVHWKHQR]]OHZLWKPHWKDQRODQGGU\WKHQR]]OH ZLWKQLWURJHQJDV 5-28 Finnigan: LCQ MS Detector Hardware Manual

162 MS DETECTOR MAINTENANCE API Source Maintenance Reassembling the ESI Probe 7RUHDVVHPEOHWKH(6,SUREHWKDWLVWRUHLQVWDOOWKH(6,VDPSOH WXEHQR]]OHQHHGOHDQGQHHGOHVHDOSURFHHGDVIROORZVVHH )LJXUH 1. Ensure the following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innigan: LCQ MS Detector Hardware Manual 5-29

163 MS DETECTOR MAINTENANCE API Source Maintenance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o on to the next topic: Reinstalling the ESI Probe Finnigan: LCQ MS Detector Hardware Manual

164 MS DETECTOR MAINTENANCE API Source Maintenance Figure 5-5. ESI nozzle, showing the proper position of the sample tube 7RLQVWDOOWKHROGVDPSOHWXEHDQGWKH(6,QR]]OHQHHGOH DQGQHHGOHVHDOSURFHHGDVIROORZV D E F G H I,QVHUWWKHROGVDPSOHWXEH>ZLWKDLQ,'.HO) IHUUXOH31DQGVDPSOHLQOHWILWWLQJ 31RQLW@WKURXJKWKHVDPSOHLQOHWDQGRXW WKHH[LWHQGRIWKH(6,SUREH,QVHUWWKHHQWUDQFHHQGRIWKH(6,QHHGOHLQWRWKHQHHGOH VHDO6HH)LJXUHIRUWKHSURSHURULHQWDWLRQRIWKH QHHGOHVHDO,QVHUWWKH(6,QHHGOHDQGQHHGOHVHDORYHUWKHVDPSOH WXEH6HDWWKH(6,QHHGOHDQGQHHGOHVHDOLQWKH(6, PDQLIROG (QVXUHWKDWWKHVDPSOHWXEHSURWUXGHVDWOHDVWFP LQSDVWWKHHQGRIWKHQHHGOH7LJKWHQWKHVDPSOH LQOHWILWWLQJVOLJKWO\EXWQRWFRPSOHWHO\ 7KUHDGWKH(6,QR]]OHRYHUWKHVDPSOHWXEHDQG(6, QHHGOHDQGLQWRWKH(6,PDQLIROG :LWKDLQZUHQFKJHQWO\WLJKWHQWKH(6,QR]]OH XQWLOLWLVDOLWWOHPRUHWKDQILQJHUWLJKW Finnigan: LCQ MS Detector Hardware Manual 5-31

165 MS DETECTOR MAINTENANCE API Source Maintenance J K 3XOOWKHVDPSOHWXEHEDFNZDUGVXQWLOLWLVPPLQVLGH WKHH[LWHQGRIWKH(6,QHHGOH6HH)LJXUH 7LJKWHQWKHVDPSOHLQOHWILWWLQJVHFXUHO\WRKROGWKH VDPSOHWXEHLQSODFH 1RWH7KHVDPSOHWXEHPD\PRYHIRUZDUGZKHQ\RXWLJKWHQWKH VDPSOHLQOHWILWWLQJ(QVXUHWKDWWKHVDPSOHWXEHLVUHWUDFWHGLQWR WKH(6,QHHGOHDSSUR[LPDWHO\PP,IQHFHVVDU\ORRVHQWKH ILWWLQJDQGUHSRVLWLRQWKHVDPSOHWXEH Reinstalling the ESI Probe 7RUHLQVWDOOWKH(6,SUREHLQWRWKH(6,IODQJHGRWKHIROORZLQJ (QVXUHWKDWWKHLQ,'2ULQJ31WKDW VHDOVWKH(6,SUREHWRWKH(6,IODQJHLVVHDWHGSURSHUO\LQWKH JURRYHLQWKH(6,IODQJH6HH)LJXUHRQSDJH 3DVVWKH(6,VDPSOHWXEHWKURXJKWKH(6,IODQJHDQG LQVWDOOWKH(6,SUREHLQWRWKH(6,IODQJH 3XOOWKH(6,SUREHEDFNDJDLQVWWKH(6,IODQJH7LJKWHQ WKHSUREHUHWDLQHUEROWWKDWKROGVWKH(6,SUREHWRWKH (6,IODQJH $WWDFKWKHWUDQVIHUOLQHILWWLQJWRWKHJURXQGHGILWWLQJKROGHU RQWKH(6,IODQJH 5. Reinstall the ESI probe assembly on the LCQ as described in the topic Installing the ESI Probe Assembly in the Changing API Probe Assemblies chapter, or place the ESI probe assembly in its storage container Finnigan: LCQ MS Detector Hardware Manual

166 MS DETECTOR MAINTENANCE API Source Maintenance Maintaining the API Stack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hutting Down the System 6KXWGRZQDQGYHQWWKHV\VWHPDVGHVFULEHGLQWKHWRSLF6KXWWLQJ 'RZQWKH6\VWHP&RPSOHWHO\LQWKH6\VWHP6KXWGRZQ 6WDUWXSDQG5HVHWFKDSWHU *RRQWRWKHQH[WWRSLF5HPRYLQJWKH$3,6WDFN Finnigan: LCQ MS Detector Hardware Manual 5-33

167 MS DETECTOR MAINTENANCE API Source Maintenance Removing the API Stack 7RUHPRYHWKH$3,VWDFNSURFHHGDVIROORZV :$51,1*0DNHVXUHWKDWWKH/&4SRZHUFRUGLVXQSOXJJHG EHIRUH\RXSURFHHG :$5181*'DV/&4PX YRP1HW]JHWUHQQWZHUGHQEHYRU LUJHQGZHOFKH(LQJULIIHYRUJHQRPQHQZHUGHQ $9(57,66(0(179HULILH]TXHOHFRUGRQGªDOLPHQWDWLRQGX /&4VRLWGpEUDQFKpGHODSULVHGHFRXUDQWDYDQWGHSURFpGHU 1. Loosen the two flange retainer bolts that secure the API flange to the spray shield. 2. Pull back the API probe assembly from the spray shield. 3. Disconnect the waste line from the spray shield. 4. Disconnect the API stack electrical cable from the spray shield by turning the tab on the end of the cable counterclockwise (toward you) and then pulling the cable free. See Figure Grasp the spray shield with both hands and carefully pull it and the API stack free from the vacuum manifold. Place the API stack on a clean surface with the spray shield down. Allow the API stack to cool to ambient temperature before you disassemble the API stack Finnigan: LCQ MS Detector Hardware Manual

168 MS DETECTOR MAINTENANCE API Source Maintenance 1RWH,I\RXDUHXQDEOHWRGLVORGJHWKHVSUD\VKLHOGIURPWKH YDFXXPPDQLIROGUHDWWDFKWKH$3,IODQJHWRWKHVSUD\VKLHOGDQG WKHQSXOOWKHIODQJHDZD\IURPWKHYDFXXPPDQLIROG 7KH$3,VWDFNLVVKRZQLQ)LJXUH*RRQWRWKHQH[WWRSLF 'LVDVVHPEOLQJWKH$3,6WDFN TUBE LENS AND SKIMMER MOUNT TUBE LENS LEAD SKIMMER TUBE LENS SKIMMER LEAD HEATED CAPILLARY HEATED CAPILLARY MOUNT Figure 5-6. API stack 3.85-IN. O-RING CONNECTO R RETAINER SPRAY SHIELD HEATED CAPILLARY CABLE CONNECTOR Finnigan: LCQ MS Detector Hardware Manual 5-35

169 MS DETECTOR MAINTENANCE API Source Maintenance Disassembling the API Stack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innigan: LCQ MS Detector Hardware Manual

170 MS DETECTOR MAINTENANCE API Source Maintenance Figure 5-7. Exploded view of the API stack Finnigan: LCQ MS Detector Hardware Manual 5-37

171 MS DETECTOR MAINTENANCE API Source Maintenance Cleaning the API Stack Components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leaning the Tube Lens &OHDQWKHLQQHUERUHRIWKHWXEHOHQVZLWK+3/&JUDGHPHWKDQRO DQGDFRWWRQWLSSHGDSSOLFDWRUVZDE Cleaning the Skimmer /RRNDWWKHWLSRIWKHFRQHRQWKHVNLPPHUIRUDUHJLRQWKDWVKRZV GLVFRORUDWLRQGXHWRFRQWDPLQDWLRQ7KHRIID[LVSDWWHUQWKDW\RX VHHLVDUHVXOWRIWKHVDPSOHVROYHQWWKDWH[LWVIURPWKHRIID[LV KHDWHGFDSLOODU\8VHPHWKDQRODQGDFRWWRQWLSSHGDSSOLFDWRURU.LPZLSHWRFOHDQWKHHQWUDQFHDQGH[LWVLGHVRIWKHVNLPPHU Cleaning the Heated Capillary 7RFOHDQWKHKHDWHGFDSLOODU\GRWKHIROORZLQJ 1. Use methanol and a Kimwipe to clean the entrance end, exit end, and exterior of the heated capillary. 2. Clear the bore of the heated capillary by inserting and withdrawing the 28 gauge, 10-in hypodermic tube (P/N ) included in your accessory kit. 3. Flush the bore of the heated capillary with methanol Finnigan: LCQ MS Detector Hardware Manual

172 MS DETECTOR MAINTENANCE API Source Maintenance 4. Dry the bore of the heated capillary with nitrogen gas Cleaning the Spray Shield To clean the spray shield, wipe the inside and outside of the spray shield with methanol and a Kimwipe. *RRQWRWKHQH[WWRSLF5HDVVHPEOLQJWKH$3,6WDFN Reassembling the API Stack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innigan: LCQ MS Detector Hardware Manual 5-39

173 MS DETECTOR MAINTENANCE API Source Maintenance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innigan: LCQ MS Detector Hardware Manual

174 MS DETECTOR MAINTENANCE API Source Maintenance Finnigan: LCQ MS Detector Hardware Manual 5-41

175 MS DETECTOR MAINTENANCE API Source Maintenance Figure 5-8. Cross sectional view of the entrance end of the API stack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einstalling the API Stack 7RUHLQVWDOOWKH$3,VWDFNSURFHHGDVIROORZV :LWKDVFUHZGULYHUORRVHQWKHWZRVFUHZVWKDWVHFXUHWKHOLG WRWKHFDSLOODU\VNLPPHUUHJLRQRIWKHYDFXXPPDQLIROG6HH )LJXUH5HPRYHWKHOLG 5-42 Finnigan: LCQ MS Detector Hardware Manual

176 MS DETECTOR MAINTENANCE API Source Maintenance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tarting Up the System 6WDUWXSWKHV\VWHPDVGHVFULEHGLQWKHWRSLF6WDUWLQJ8SWKH 6\VWHP$IWHUD&RPSOHWH6KXWGRZQLQWKH6\VWHP 6KXWGRZQ6WDUWXSDQG5HVHWFKDSWHU Finnigan: LCQ MS Detector Hardware Manual 5-43

177 MS DETECTOR MAINTENANCE Cleaning the Ion Optics and Mass Analyzer 5.4 Cleaning the Ion Optics and Mass Analyzer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hutting Down the System 6KXWGRZQDQGYHQWWKHV\VWHPDVGHVFULEHGLQWKHWRSLF6KXWWLQJ 'RZQWKH6\VWHP&RPSOHWHO\LQWKH6\VWHP6KXWGRZQ 6WDUWXSDQG5HVHWFKDSWHU 5-44 Finnigan: LCQ MS Detector Hardware Manual

178 MS DETECTOR MAINTENANCE Cleaning the Ion Optics and Mass Analyzer :$51,1*0DNHVXUHWKDWWKH/&4SRZHUFRUGLVXQSOXJJHG EHIRUH\RXSURFHHG :$5181*'DV/&4PX YRP1HW]JHWUHQQWZHUGHQEHYRU LUJHQGZHOFKH(LQJULIIHYRUJHQRPQHQZHUGHQ $9(57,66(0(179HULILH]TXHOHFRUGRQGªDOLPHQWDWLRQGX /&4VRLWGpEUDQFKpGHODSULVHGHFRXUDQWDYDQWGHSURFpGHU *RRQWRWKHQH[WWRSLF5HPRYLQJWKH7RS&RYHURIWKH06 'HWHFWRU Removing the Top Cover of the MS Detector 5HPRYHWKHWRSFRYHURIWKH06GHWHFWRUDVIROORZV 'LVFRQQHFWDQ\WXELQJEHWZHHQWKHV\ULQJHSXPSDQGWKH$3, VRXUFH 2SHQWKHOHIWDQGULJKWIURQWGRRUVRIWKH06GHWHFWRUE\ ORRVHQLQJWKHLQ$OOHQVFUHZRQWKHULJKWIURQWGRRUZLWK DQ$OOHQZUHQFK 3. Loosen the four fasteners that hold the top cover to the MS detector chassis. The fasteners are located in the upper right and left corners of the chassis. 4. Slide the top cover back by about 1.25 cm (0.5 in.). 5. With one hand under the center of the top cover, lift the top cover up and away from the MS detector. *RRQWRWKHQH[WWRSLF5HPRYLQJWKH7RS&RYHU3ODWHRIWKH 9DFXXP0DQLIROG Finnigan: LCQ MS Detector Hardware Manual 5-45

179 MS DETECTOR MAINTENANCE Cleaning the Ion Optics and Mass Analyzer Removing the Top Cover Plate of the Vacuum Manifold <RXQHHGWRUHPRYHWKHWRSFRYHUSODWHRIWKHYDFXXPPDQLIROGWR DFFHVVWKHLRQRSWLFVPDVVDQDO\]HUDQGLRQGHWHFWLRQV\VWHP 7KHWRSFRYHUSODWHLVKHOGLQSODFHE\JUDYLW\DQGE\WKHDLU SUHVVXUHGLIIHUHQWLDOEHWZHHQWKHYDFXXPPDQLIROGDQG DWPRVSKHULFSUHVVXUH6L[FDEOHVDQGRQHJDVOLQHDUHFRQQHFWHG WRWKHWRSFRYHUSODWH6HH)LJXUH 7RUHPRYHWKHWRSFRYHUSODWHSURFHHGDVIROORZV 'LVFRQQHFWDW$1$/$8;,1WKHRFWDSROHVFDEOHWKDW FRPHVIURPWKH$QDO\]HU$X[LOLDU\3&% 2. Disconnect (at ANALYZER) the lenses cable that comes from the System Control PCB. 3. Disconnect (at ANAL. AUX 2 IN and ANAL. AUX 3 IN) the two endcap electrode cables that come from the Analyzer Auxiliary PCB. 4. Disconnect (at ACQU/DSP) the electrometer cable. (If necessary, use a small screw driver to loosen the screws that secure the cable.) 5. Disconnect (at MULT) the electron multiplier high voltage cable that comes from the electron multiplier power supply. 6. Use a 7/16-in. open-end wrench to disconnect the helium damping gas line from the fitting. &DUHIXOO\OLIWWKHWRSFRYHUSODWHVWUDLJKWXSE\LWVWZR KDQGOHV7DNHFDUHQRWWRGDPDJHWKHFRPSRQHQWVRQWKH XQGHUVLGHRIWKHFRYHUSODWH3ODFHWKHFRYHUSODWHXSVLGH GRZQVXSSRUWHGRQLWVKDQGOHVRQDIODWVXUIDFH &RYHUWKHRSHQLQJLQWKHWRSRIWKHYDFXXPPDQLIROGZLWKD ODUJHOLQWIUHHWLVVXH *RRQWRWKHQH[WWRSLF5HPRYLQJWKH,RQ2SWLFVDQG0DVV $QDO\]HU 5-46 Finnigan: LCQ MS Detector Hardware Manual

180 MS DETECTOR MAINTENANCE Cleaning the Ion Optics and Mass Analyzer DAMPING GAS LINE ELECTRON MULTIPLIER HIGH ELECTROMETER CABLE ENDCAP ELECTRODE CABLES LENSES CABLE OCTAPOLES CABLE Figure 5-9. Electrical connections and damping gas line connection to the top cover plate of the vacuum manifold Removing the Ion Optics and Mass Analyzer 7KHLRQRSWLFVDQGPDVVDQDO\]HUDUHPRXQWHGRQDEDIIOHRQWKH XQGHUVLGHRIWKHWRSFRYHUSODWHRIWKHYDFXXPPDQLIROG6HH )LJXUHDQG)LJXUH 8VHWKHIROORZLQJSURFHGXUHWRUHPRYHWKHLRQRSWLFVDQGPDVV DQDO\]HUIURPWKHWRSFRYHUSODWH5HIHUWR)LJXUHDQG)LJXUH IRUWKHORFDWLRQRIWKHLRQRSWLFVDQGPDVVDQDO\]HU FRPSRQHQWV Finnigan: LCQ MS Detector Hardware Manual 5-47

181 MS DETECTOR MAINTENANCE Cleaning the Ion Optics and Mass Analyzer DAMPING GAS LINE SECOND OCTAPOLE LEADS (PINS 1 & 3) FIRST OCTAPOLE LEADS (PINS 1 & 3) EXIT LENS LEAD (PIN 2) EXIT ENDCAP ELECTRODE LEAD (PIN 4) INTEROCTAPOLE LENS LEAD (PIN 7) ENTRANCE ENDCAP ELECTRODE LEAD (PIN 5) Figure Mass analyzer and ion optics, showing electrical and damping gas line connections 1RWH:HDUFOHDQOLQWIUHHQ\ORQRUFRWWRQJORYHVZKHQ\RX KDQGOHWKHLRQRSWLFVDQGPDVVDQDO\]HUFRPSRQHQWV 1. Prepare a clean work area by covering the area with lint-free paper. Place each part on the paper as you remove it. 2. Disconnect the two electrical leads to the first octapole. See Figure Hold the octapole mount with one hand; loosen and remove the two thumb screws that hold the octapole mount to the baffle on the top cover plate of the vacuum manifold. See Figure Finnigan: LCQ MS Detector Hardware Manual

182 MS DETECTOR MAINTENANCE Cleaning the Ion Optics and Mass Analyzer MASS ANALYZER SECOND OCTAPOLE BAFFLE THUMB SCREWS 8-PIN FEED- THROUGH ANALYZER MOUNT FIRST OCTAPOLE OCTAPOLE MOUNT Figure Mass analyzer and ion optics, mounted to the baffle on the top cover plate of the vacuum manifold 1. Remove the first octapole and octapole mount. 2. Disconnect the electrical lead to the interoctapole lens. Remove the interoctapole lens (Figure 5-10). 3. Disconnect the electrical leads to the second octapole and to the exit lens, exit endcap electrode, and entrance endcap electrode of the mass analyzer (Figure 5-10). 4. Disconnect the damping gas line from the nipple on the exit endcap electrode by pulling the line free from the nipple (Figure 5-10). 5. Hold the mass analyzer with one hand; loosen the two thumb screws that hold the analyzer mount to the baffle (Figure 5-11). Finnigan: LCQ MS Detector Hardware Manual 5-49

183 MS DETECTOR MAINTENANCE Cleaning the Ion Optics and Mass Analyzer 6. With one hand holding the mass analyzer and the other hand holding the analyzer mount, lift the mass analyzer, second octapole, and analyzer mount out and away from the baffle on the top cover plate. Be careful not to touch the electron multiplier with the mass analyzer. This could damage the electropolished surface. *RRQWRWKHQH[WWRSLF'LVDVVHPEOLQJWKH,RQ2SWLFVDQG 0DVV$QDO\]HU Disassembling the Ion Optics and Mass Analyzer 7RGLVDVVHPEOHWKHLRQRSWLFVDQGPDVVDQDO\]HUSURFHHGDV IROORZV5HIHUWR)LJXUHDQG)LJXUHIRUWKHORFDWLRQRI WKHLRQRSWLFVDQGPDVVDQDO\]HUFRPSRQHQWV 1. Remove the first octapole from the octapole mount. 2. Remove the second octapole from the analyzer mount. 3. Disassemble the mass analyzer as follows: a. Remove the exit lens by pulling the exit lens out of the exit lens sleeve. b. Remove the exit lens sleeve by squeezing the sleeve and pulling it out of the recess in the exit endcap electrode. c. Unscrew and remove the two nuts from the posts. d. Remove the two spring washers from the posts. e. Remove the exit endcap electrode from the posts. f. Remove the two spacer rings and the ring electrode. g. Remove the entrance endcap electrode from the posts. h. Unscrew and remove the two posts from the analyzer mount. *RRQWRWKHQH[WWRSLF&OHDQLQJWKH,RQ2SWLFVDQG0DVV $QDO\]HU3DUWV 5-50 Finnigan: LCQ MS Detector Hardware Manual

184 MS DETECTOR MAINTENANCE Cleaning the Ion Optics and Mass Analyzer Figure Exploded view of the ion optics Finnigan: LCQ MS Detector Hardware Manual 5-51

185 MS DETECTOR MAINTENANCE Cleaning the Ion Optics and Mass Analyzer Figure Exploded and assembled views of the mass analyzer 5-52 Finnigan: LCQ MS Detector Hardware Manual

186 MS DETECTOR MAINTENANCE Cleaning the Ion Optics and Mass Analyzer Cleaning the Ion Optics and Mass Analyzer Parts 8VHWKHIROORZLQJSURFHGXUHWRUHPRYHFRQWDPLQDWLRQIURPWKHLRQ RSWLFVDQGPDVVDQDO\]HUSDUWV&OHDQHDFKSDUWLQWXUQ$IWHU FOHDQLQJSODFHHDFKSDUWRQDFOHDQOLQWIUHHVXUIDFH &DXWLRQ7DNHFDUHQRWWRFKLSVFUDWFKRUEUHDNWKHVSDFHUULQJV RIWKHPDVVDQDO\]HU7DNHFDUHQRWWREXPSRUMDUWKHRFWDSROHV 'RQRWSODFHWKHRFWDSROHVLQDQXOWUDVRQLFFOHDQHU 1RWH:KHQ\RXFOHDQWKHLRQRSWLFVDQGPDVVDQDO\]HUSDUWVSD\ SDUWLFXODUDWWHQWLRQWRWKHLQVLGHVXUIDFHV 1. With a soft tooth brush or lint-free swab, scrub the ion optics or mass analyzer part with a solution of detergent and water. 5LQVHWKHSDUWZLWKWDSZDWHUWRUHPRYHWKHGHWHUJHQW 5LQVHWKHSDUWZLWKGLVWLOOHGZDWHU 3ODFHWKHSDUWLQDWDOOEHDNHUDQGLPPHUVHLWFRPSOHWHO\LQ +3/&JUDGHPHWKDQRO0RYHWKHSDUWXSDQGGRZQLQWKH PHWKDQROIRUV 1RWH:HDUFOHDQOLQWIUHHQ\ORQRUFRWWRQJORYHVWRKDQGOHWKH SDUWVDIWHU\RXFOHDQWKHPLQPHWKDQRO 5HPRYHWKHSDUWIURPWKHPHWKDQROEDWKWKHQULQVHLW WKRURXJKO\ZLWKIUHVKPHWKDQRO 'U\WKHSDUWZLWKDUDSLGVWUHDPRIQLWURJHQJDV,QVSHFWHDFKSDUWIRUFRQWDPLQDWLRQDQGGXVW,IQHFHVVDU\ UHSHDWWKHFOHDQLQJSURFHGXUH Finnigan: LCQ MS Detector Hardware Manual 5-53

187 MS DETECTOR MAINTENANCE Cleaning the Ion Optics and Mass Analyzer $IWHUDOOLRQRSWLFVDQGPDVVDQDO\]HUSDUWVDUHFOHDQDQGGU\JR RQWRWKHQH[WWRSLF5HDVVHPEOLQJWKH,RQ2SWLFVDQG0DVV $QDO\]HU Reassembling the Ion Optics and Mass Analyzer 8VHWKHIROORZLQJSURFHGXUHWRUHDVVHPEOHWKHLRQRSWLFVDQGPDVV DQDO\]HU5HIHUWR)LJXUHDQG)LJXUHRQSDJHVDQG 1RWH:HDUFOHDQOLQWIUHHQ\ORQRUFRWWRQJORYHVZKHQ\RX KDQGOHFRPSRQHQWVRIWKHPDVVDQDO\]HU 1. Reassemble the mass analyzer as follows: a. Reinstall the two posts by screwing them by hand into the analyzer mount. (Both ends are the same.) b. Reinstall the entrance endcap electrode onto the posts. (The entrance endcap electrode is the one without the damping gas nipple.) Ensure that the electrode is oriented such that the convex surface faces away from the analyzer mount. Also, the opening in which the pin on the end of the electrical lead inserts should be close to the top cover plate when the analyzer mount is installed on the top cover plate. See Figure 5-10 on page &DXWLRQ+DQGOHWKHVSDFHUULQJVFDUHIXOO\'RQRWVFUDSHWKH VSDFHUULQJVDJDLQVWDQ\PHWDOVXUIDFHV0HWDOGHSRVLWVRQWKH VXUIDFHVRIWKHVSDFHUULQJVPLJKWFDXVHWKH5)YROWDJHWRDUF DFURVVWKHVSDFHUULQJVWRWKHHQGFDSV'RQRWRYHUWLJKWHQWKH PDVVDQDO\]HUQXWV c. Place a spacer ring into the groove in the entrance endcap electrode. d. Reinstall the ring electrode onto the spacer ring so that the spacer ring is held securely between the electrodes. The 5-54 Finnigan: LCQ MS Detector Hardware Manual

188 MS DETECTOR MAINTENANCE Cleaning the Ion Optics and Mass Analyzer orientation of the ring electrode is unimportant. (Both sides are the same.) e. Reinstall the second spacer ring into the groove in the ring electrode. f. Reinstall the exit endcap electrode (the one with the damping gas nipple) on the posts such that the spacer ring is held in place between the ring electrode and the exit endcap electrode. Make sure that the electrode is oriented such that the convex surface faces the spacer ring. Also, the damping gas nipple should point toward the top cover plate when the analyzer mount is installed on the top cover plate. g. Inspect the mass analyzer assembly. Ensure that all the parts are aligned properly and that they all fit together snugly. h. Reinstall the two spring washers on the posts such that the convex side of the washer is toward the exit endcap electrode. i. Reinstall the two nuts onto the posts and tighten the nuts by hand until they are finger tight. Do not overtighten the nuts. j. Squeeze the exit lens sleeve and insert it into the recess in the exit endcap electrode. See Figure 5-13 on page 5-52 for the proper orientation of the exit lens sleeve. k. Insert the exit lens into the exit lens sleeve such that the lead pin on the exit lens points in the same direction as the 8-pin feedthrough when the analyzer mount is installed on the top cover plate. See Figure 5-10 on page Make sure that the exit lens lead pin does not contact the nut on the end of the mass analyzer post. 2. Insert one of the octapoles into the octapole mount. (Both octapoles are the same.) 3. Insert the other octapole through the cylindrical end of the analyzer mount until it seats in the entrance endcap electrode of the mass analyzer. Turn the octapole until the lead pins are on the same side as the 4-pin feedthrough (when the analyzer mount is mounted on the top cover plate). *RRQWRWKHQH[WWRSLF5HLQVWDOOLQJWKH,RQ2SWLFVDQG0DVV $QDO\]HU Finnigan: LCQ MS Detector Hardware Manual 5-55

189 MS DETECTOR MAINTENANCE Cleaning the Ion Optics and Mass Analyzer Reinstalling the Ion Optics and Mass Analyzer 8VHWKHIROORZLQJSURFHGXUHWRUHLQVWDOOWKHLRQRSWLFVDQGPDVV DQDO\]HURQWRWKHWRSFRYHUSODWHRIWKHYDFXXPPDQLIROG 1RWH:HDUFOHDQOLQWIUHHQ\ORQRUFRWWRQJORYHVZKHQ\RX KDQGOHFRPSRQHQWVRIWKHLRQRSWLFVDQGPDVVDQDO\]HU 1. Insert the cylindrical end of the analyzer mount (with the mass analyzer and second octapole attached) into the opening in the baffle on the top cover plate of the vacuum manifold. Ensure that the open side of the analyzer mount is away from the top cover plate. See Figure Secure the analyzer mount to the baffle with the two thumb screws. 3. Insert the interoctapole lens, lead pin first, through the opening in the baffle. Turn the interoctapole lens until the lead pin is on the same side as the 8-pin feedthrough. Ensure that the second octapole is held securely between the endcap electrode and the interoctapole lens. Also ensure that the lead pins on the octapole are on the same side as the 4-pin feedthrough. 4. Attach the first octapole and octapole mount to the baffle on the top cover plate with the two thumb screws. Ensure that the interoctapole lens is held securely between the two octapoles. Also ensure that the lead pins on the octapole are on the same side as the 4-pin feedthrough. 5. Inspect the ion optics. Ensure that all the parts are aligned properly and that they all fit together snugly. 6. Reconnect the two electrical leads from pins 1 and 3 of the 4-pin feedthrough (see Figure 5-10 on page 5-48) to the first octapole. (It does not matter which lead is connected to a particular lead pin of the octapole.) 7. Reconnect the electrical lead from pin 7 of the 8-pin feedthrough (Figure 5-10) to the interoctapole lens. 8. Reconnect the two electrical leads from pins 1 and 3 of the 4-pin feedthrough (Figure 5-10) to the second octapole. (It does 5-56 Finnigan: LCQ MS Detector Hardware Manual

190 MS DETECTOR MAINTENANCE Cleaning the Ion Optics and Mass Analyzer not matter which lead is connected to a particular lead pin of the octapole.) 9. Reconnect the electrical lead from pin 5 of the 8-pin feedthrough (Figure 5-10) to the entrance endcap electrode by inserting the pin on the end of the lead into the socket in the electrode. 10. Reconnect the electrical lead from pin 4 of the 8-pin feedthrough (Figure 5-10) to the exit endcap electrode by inserting the pin on the end of the lead into the socket in the electrode. 11. Reconnect the electrical lead from pin 2 of the 8-pin feedthrough (Figure 5-10) to the exit lens. Ensure that the exit lens lead pin does not contact the nut. 12. Reconnect the damping gas line to the nipple on the exit endcap electrode. 1RWH&KHFNDOOOHDGVDQGHQVXUHWKDWWKH\DUHVHFXUHDQGWKDW WKH\JRWRWKHSURSHUHOHFWURGHV Go on to the next topic: Cleaning the Ion Detection System Cleaning the Ion Detection System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innigan: LCQ MS Detector Hardware Manual 5-57

191 MS DETECTOR MAINTENANCE Cleaning the Ion Optics and Mass Analyzer Reinstalling the Top Cover Plate of the Vacuum Manifold 8VHWKHIROORZLQJSURFHGXUHWRUHLQVWDOOWKHWRSFRYHUSODWHRIWKH YDFXXPPDQLIROG 1. Remove the tissue from the opening in the top of the vacuum manifold. 2. Check the O-ring that surrounds the opening for signs of wear, and replace it if necessary (P/N ). Make sure that the O-ring is seated properly. 1RWH3HULRGLFDOO\UHPRYHDQ\FRQWDPLQDWLRQWKDWPLJKWEHRQ WKHLQQHUZDOOVRIWKHPDQLIROGE\ZLSLQJWKHLQQHUZDOOVZLWKD OLQWIUHHWLVVXHVRDNHGLQ+3/&JUDGHPHWKDQRO8VHDFRWWRQ WLSSHGDSSOLFDWRUVRDNHGLQPHWKDQROWRFOHDQDURXQGLQOHWVDQG IHHGWKURXJKV 8. Carefully lift the top cover plate up by its two handles and turn it over. Orient the top cover plate such that the electron multiplier is over the conversion dynode. Carefully insert the guide posts on the underside of the top cover plate into the guide holes in the vacuum manifold. Slowly lower the cover plate onto the opening in the vacuum manifold. Take care not to damage the components on the underside of the cover plate. Ensure that the cover plate is seated properly on the vacuum manifold. 8VHDLQRSHQHQGZUHQFKWRUHFRQQHFWWKHKHOLXP GDPSLQJJDVOLQHWRWKHILWWLQJ6HH)LJXUHRQSDJH 5HFRQQHFWDW$1$/$8;,1WKHRFWDSROHVFDEOHWKDW FRPHVIURPWKH$QDO\]HU$X[LOLDU\3&% 11. Reconnect (at ANALYZER) the lenses cable that comes from the System Control PCB. 12. Reconnect (at ANAL. AUX 2 IN and ANAL. AUX 3 IN) the two endcap electrode cables that come from the Analyzer Auxiliary PCB. 13. Reconnect (at ACQU/DSP) the electrometer cable Finnigan: LCQ MS Detector Hardware Manual

192 MS DETECTOR MAINTENANCE Cleaning the Ion Optics and Mass Analyzer 5HFRQQHFWDW08/7WKHHOHFWURQPXOWLSOLHUKLJKYROWDJH FDEOHWKDWFRPHVIURPWKHHOHFWURQPXOWLSOLHUSRZHUVXSSO\ *RRQWRWKHQH[WWRSLF5HLQVWDOOLQJWKH7RS&RYHURIWKH06 'HWHFWRU Reinstalling the Top Cover of the MS Detector 5HLQVWDOOWKHWRSFRYHURIWKH06GHWHFWRUDVIROORZV 1. Open the left and right front doors of the MS detector. 2. With one hand under the center of the top cover, place the top cover over the MS detector such that the front of the cover is about 1.25 cm (0.5 in.) behind the front of the MS detector. 3. Slide the cover forward until it is flush with the front doors (when they are closed). 7LJKWHQE\KDQGWKHIRXUIDVWHQHUVWRVHFXUHWKHWRSFRYHUWR WKHFKDVVLV 5. Close the left and right front doors of the MS detector. 6. Reconnect any tubing between the syringe pump and the API source to accomodate your instrument configuration. *RRQWRWKHQH[WWRSLF6WDUWLQJ8SWKH6\VWHP Starting Up the System 6WDUWXSWKHV\VWHPDVGHVFULEHGLQWKHWRSLF6WDUWLQJ8SWKH 6\VWHP$IWHUD&RPSOHWH6KXWGRZQLQWKH6\VWHP 6KXWGRZQ6WDUWXSDQG5HVHWFKDSWHU *RRQWRWKHQH[WWRSLF7XQLQJWKH5LQJ(OHFWURGHDQG 2FWDSROH5)9ROWDJHV Finnigan: LCQ MS Detector Hardware Manual 5-59

193 MS DETECTOR MAINTENANCE Cleaning the Ion Optics and Mass Analyzer Tuning the Ring Electrode and Octapole RF Voltages <RXQHHGWRWXQHWKHULQJHOHFWURGH5)YROWDJHDQGWKHRFWDSROH 5)YROWDJHZKHQHYHU\RXVHUYLFHWKHPDVVDQDO\]HURULRQRSWLFV <RXDOVRQHHGWRWXQHWKHVHYROWDJHVLI\RXUHSODFHDQ\HOHFWURQLF DVVHPEO\WKDWLVLQYROYHGLQSURGXFLQJWKH5)YROWDJHV<RXXVH WKH'LDJQRVWLFVSURJUDPWRWXQHWKHULQJHOHFWURGHDQGRFWDSROH 5)YROWDJHV 7RWXQHWKHULQJHOHFWURGHDQGRFWDSROH5)YROWDJHVSURFHHGDV IROORZV $OORZWKH/&4WRSXPSGRZQIRUDWOHDVWPLQDIWHUVWDUW XS 2SHQWKH7XQH3OXVZLQGRZ &KRRVHControl OnWRWXUQWKH/&42Q 2SHQWKH'LDJQRVWLFVGLDORJER[DQG*UDSKYLHZDVIROORZV D,QWKH7XQH3OXVZLQGRZFKRRVHView Display Graph ViewWRRSHQWKH*UDSKYLHZ E,QWKH7XQH3OXVZLQGRZFKRRVHDiagnostics DiagnosticsWRRSHQWKH'LDJQRVWLFVGLDORJER[ F G 6HOHFWWKHGraphWDE 5HSRVLWLRQWKH'LDJQRVWLFVGLDORJER[VRWKDWLWGRHVQRW REVFXUHWKH*UDSKYLHZ6HH)LJXUH 7XQHWKHRFWDSROH5)YROWDJHDVIROORZV D E F 6HOHFWTune octapole frequencylqwkh7hvw7\shwh[wer[ 6HOHFWWKHOnceRSWLRQEXWWRQLQWKH+RZPDQ\WLPHV JURXSER[ &OLFNRQWKHStartEXWWRQWRVWDUWWKHRFWDSROH5)YROWDJH WXQHSURJUDP$IUHTXHQF\IXQFWLRQDSSHDUVLQWKH*UDSK YLHZ6HH)LJXUH7KHPLQLPXPRIWKHIUHTXHQF\ IXQFWLRQVKRXOGOLHEHWZHHQDQGN+] 5-60 Finnigan: LCQ MS Detector Hardware Manual

194 MS DETECTOR MAINTENANCE Cleaning the Ion Optics and Mass Analyzer G :KHQWKHRFWDSROHWXQHSURJUDPLVILQLVKHG/&4GLVSOD\V WKHPHVVDJH'R\RXZDQWWRDFFHSWWKHRFWDSROH IUHTXHQF\"&OLFNRQWKHYesEXWWRQ Figure Diagnostics dialog box and Graph view Finnigan: LCQ MS Detector Hardware Manual 5-61

195 MS DETECTOR MAINTENANCE Cleaning the Ion Optics and Mass Analyzer Figure Graph view for octapole RF voltage tuning 5-62 Finnigan: LCQ MS Detector Hardware Manual

196 MS DETECTOR MAINTENANCE Cleaning the Ion Optics and Mass Analyzer 7XQHWKHULQJHOHFWURGH5)YROWDJHPRGXODWLRQDVIROORZV D E F 6HOHFWTune RF modulationlqwkh7hvw7\shwh[wer[ 6HOHFWWKHOnceRSWLRQEXWWRQLQWKH+RZPDQ\WLPHV JURXSER[ &OLFNRQWKHStartEXWWRQWRVWDUWWKHULQJHOHFWURGH5) PRGXODWLRQWXQHSURJUDP7KH*UDSKYLHZVKRXOGORRN OLNH)LJXUH 7KHVWDQGLQJZDYHUDWLRVZLWFKOLQHVKRXOGEHDW9 RYHUWKHHQWLUHUDQJH 7KHGHWHFWHG5)YROWDJHVKRXOGEHDVWUDLJKWOLQHWKDW EHJLQVDWWKHRULJLQDQGLQWHUVHFWVWKHVWDQGLQJZDYH UDWLRVZLWFKOLQHQHDUWKHKLJKHVWPDVVOLQH 7KH5)YROWDJHPRGXODWLRQVKRXOGEHDFXUYHGOLQH WKDWEHJLQVDWWKHRULJLQDQGLQWHUVHFWVWKHKLJKHVW PDVVOLQHDWDYDOXHEHWZHHQDQG9 STANDING WAVE RATIO SWITCH DETECTED RF VOLTAGE HIGHEST MASS RF VOLTAGE MODULATIO Figure Graph view for ring electrode RF voltage modulation tuning Finnigan: LCQ MS Detector Hardware Manual 5-63

197 MS DETECTOR MAINTENANCE Cleaning the Ion Optics and Mass Analyzer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une RF frequencylqwkh7hvw7\shwh[wer[7kh &RQWLQXRXVRSWLRQEXWWRQLQWKH+RZPDQ\WLPHVJURXS ER[LVDXWRPDWLFDOO\VHOHFWHG &OLFNRQWKHStartEXWWRQWRVWDUWWKHULQJHOHFWURGH5) IUHTXHQF\WXQHSURJUDP7KH*UDSKYLHZGLVSOD\V VHYHUDOWXQHIXQFWLRQVDIUHTXHQF\FXUVRUDQGD IUHTXHQF\ZLQGRZ6HH)LJXUH $OORZWKHSURJUDPWRPDNHDWOHDVWILYHSDVVHV7KHQ GHWHUPLQHZKHWKHUWKHIUHTXHQF\FXUVRUOLHVZLWKLQWKH IUHTXHQF\ZLQGRZ,IWKHIUHTXHQF\FXUVRUOLHVZLWKLQWKHIUHTXHQF\ ZLQGRZWKHQWKHULQJHOHFWURGH5)YROWDJHIUHTXHQF\ LVWXQHGSURSHUO\&OLFNRQWKHStopEXWWRQDQGH[LW IURPWKHGLDJQRVWLFVSURJUDP,IWKHIUHTXHQF\FXUVRUOLHVRXWVLGHWKHIUHTXHQF\ ZLQGRZWKHQ\RXQHHGWRPDQXDOO\DGMXVWWKHULQJ HOHFWURGH5)YROWDJHIUHTXHQF\/HDYHWKH*UDSKYLHZ GLVSOD\HG*RRQWRWKHQH[WVWHS 5-64 Finnigan: LCQ MS Detector Hardware Manual

198 MS DETECTOR MAINTENANCE Cleaning the Ion Optics and Mass Analyzer FREQUENCY CURSOR FREQUENCY WINDOW Figure Graph view for ring electrode RF voltage tuning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innigan: LCQ MS Detector Hardware Manual 5-65

199 MS DETECTOR MAINTENANCE Cleaning the Ion Optics and Mass Analyzer J &OLFNRQWKHStopEXWWRQWRVWRSWKHULQJHOHFWURGH5) YROWDJHIUHTXHQF\WXQHSURJUDP 9/16-IN. LOCK NUT TUNING STUD Figure Ring electrode RF voltage tuning stud (with air deflector removed) 5-66 Finnigan: LCQ MS Detector Hardware Manual

200 MS DETECTOR MAINTENANCE Replacing the Electron Multiplier 5.5 Replacing the Electron Multiplier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hut down and vent the system as described in the topic Shutting the System Down Completely in the System Shutdown, Startup, and Reset chapter. :$51,1*0DNHVXUHWKDWWKH/&4SRZHUFRUGLVXQSOXJJHG EHIRUH\RXSURFHHG Finnigan: LCQ MS Detector Hardware Manual 5-67

201 MS DETECTOR MAINTENANCE Replacing the Electron Multiplier :$5181*'DV/&4PX YRP1HW]JHWUHQQWZHUGHQEHYRU LUJHQGZHOFKH(LQJULIIHYRUJHQRPQHQZHUGHQ $9(57,66(0(179HULILH]TXHOHFRUGRQGªDOLPHQWDWLRQGX /&4VRLWGpEUDQFKpGHODSULVHGHFRXUDQWDYDQWGHSURFpGHU 2. Remove the top cover of the MS detector as described in the topic Removing the Top Cover of the MS Detector on page Remove the top cover plate of the vacuum manifold as described in the topic Removing the Top Cover Plate of the Vacuum Manifold on page RWH:HDUFOHDQOLQWIUHHQ\ORQRUFRWWRQJORYHVZKHQ\RX KDQGOHWKHHOHFWURQPXOWLSOLHUFRPSRQHQWV 4. With an Allen wrench, remove the two socket-head screws that hold the electron multiplier support to the top cover plate of the vacuum manifold. See Figure With one hand hold the high voltage tube and with the other hand hold the electron multiplier support. Then, detach the high voltage tube from the high voltage feedthrough in the top cover plate and remove the electron multiplier as a unit. (The anode remains in the anode feedthough in the top cover plate.) 6. Remove the anode from the anode feedthrough by unscrewing it counterclockwise by hand. 7. Install a new anode (P/N ) in the anode feedthrough in the top cover plate by screwing it clockwise by hand Finnigan: LCQ MS Detector Hardware Manual

202 MS DETECTOR MAINTENANCE Replacing the Electron Multiplier If you want to replace the entire electron multiplier, install a new electron multiplier (P/N ) in the next step. If you want to replace only the cathode, install the old electron multiplier in the next step. &DXWLRQ%HFDUHIXOQRWWRGDPDJHWKHVXUIDFHRIWKHHOHFWURQ PXOWLSOLHUVKLHOG7KHHOHFWURQPXOWLSOLHUVKLHOGKDVEHHQ HOHFWURSROLVKHGWRSUHYHQWILHOGHPLVVLRQ 8. With one hand holding the high voltage tube and the other hand holding the electron multiplier support, install the electron multiplier on the top cover plate. Ensure that the high voltage tube is properly inserted in the high voltage feedthrough and that the screw holes in the electron multiplier support are aligned with the screw holes in the top cover plate. Finnigan: LCQ MS Detector Hardware Manual 5-69

203 MS DETECTOR MAINTENANCE Replacing the Electron Multiplier Figure Exploded view of the electron multiplier, showing the anode 5HLQVWDOOWKHWZRVRFNHWKHDGVFUHZVWKDWVHFXUHWKHHOHFWURQ PXOWLSOLHUVXSSRUWWRWKHWRSFRYHUSODWH7LJKWHQWKHVFUHZV ZLWKDQ$OOHQZUHQFK,I\RXLQVWDOOHGDQHZHOHFWURQPXOWLSOLHULQVWHSJRWR VWHS,I\RXZDQWWRUHSODFHWKHFDWKRGHJRRQWRWKHQH[WVWHS 10. To replace the cathode, proceed as follows. See Figure a. With a Phillips screwdriver, loosen (but do not remove) the two screws that secure the support plates to the high voltage ring Finnigan: LCQ MS Detector Hardware Manual

204 MS DETECTOR MAINTENANCE Replacing the Electron Multiplier b. With one hand, hold the high voltage tube. With the other hand, hold the high voltage ring. Then, detach the high voltage tube from the high voltage feedthrough and remove the electron multiplier. Place it on a clean surface. (The electron multiplier support and the support plates should remain attached to the top cover plate.) c. Turn the assembly over. With a Phillips screwdriver, remove the two screws that secure the electron multiplier shield to the high voltage ring. d. Remove the electron multiplier shield and cathode from the high voltage ring. e. Insert the narrow end of a new cathode (P/N ) first through the spring washer and then through the high voltage ring. f. Place the electron multiplier shield over the wide end of the cathode such that the screw holes in the electron multiplier shield are aligned with the screw holes in the high voltage ring. g. Hold the high voltage ring and electron multiplier shield together to depress the spring washer. Secure the high voltage ring to the electron multiplier shield by using the two Phillips-head screws. (The cathode should be held in place between the high voltage ring and the electron multiplier shield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innigan: LCQ MS Detector Hardware Manual 5-71

205 MS DETECTOR MAINTENANCE Replacing the Electron Multiplier Figure Exploded view of the electron multiplier, showing the cathode 13. Start up the LCQ system as described in the topic Starting Up the System After a Complete Shutdown in the System Shutdown, Startup, and Reset chapter. 14. Set the electron multiplier voltage to -800 V as follows: a. From the Tune Plus window, choose Diagnostics Diagnostics. b. Select the Graphs tab. c. In the Set Device Value option box, select Multiplier (V) Finnigan: LCQ MS Detector Hardware Manual

206 MS DETECTOR MAINTENANCE Replacing the Electron Multiplier d. In the text box to the right of the Set Device Value option box, enter e. Click on the Set button to set the electron multiplier voltage to -800 V. f. Click on the OK button to return to Tune Plus. &DOLEUDWHWKHHOHFWURQPXOWLSOLHUYROWDJHDVIROORZV D E F G H $OORZWKHV\VWHPWRSXPSGRZQIRUDWOHDVWRQHKRXU EHIRUH\RXWXUQRQWKHKLJKYROWDJHV 6HWXSIRUWKHLQIXVLRQRIWKHWXQLQJVROXWLRQLQWRWKH06 GHWHFWRUDVGHVFULEHGLQWKH/&42SHUDWRUªV0DQXDO )URPWKH7XQH3OXVZLQGRZFKRRVHControl Calibrate 7KH&DOLEUDWHGLDORJER[DSSHDUV &OLFNRQWKHSemi-AutomaticWDE 6HOHFWWKH(OHFWURQ0XOWLSOLHU*DLQRSWLRQ&OLFNRQWKH StartEXWWRQWRVWDUWWKHPXOWLSOLHUJDLQSURFHGXUH $IWHUWKH(OHFWURQ0XOWLSOLHU*DLQSURJUDPLVILQLVKHGVHWXS IRU(6,RU$3&,RSHUDWLRQDVGHVFULEHGLQWKH/&4 2SHUDWRUªV0DQXDO Finnigan: LCQ MS Detector Hardware Manual 5-73

207 MS DETECTOR MAINTENANCE Purging the Oil in the Rotary-Vane Pump 5.6 Purging the Oil in the Rotary-Vane Pump <RXQHHGWRSXUJHGHFRQWDPLQDWHWKHRLOLQWKHURWDU\YDQH SXPSRQDGDLO\EDVLVWRUHPRYHZDWHUDQGRWKHUGHVROYHG FKHPLFDOVIURPWKHSXPSRLO:DWHUDQGRWKHUFKHPLFDOVLQWKH URWDU\YDQHSXPSFDQFDXVHFRUURVLRQDQGGHFUHDVHWKHOLIHWLPHRI WKHSXPS$JRRGWLPHWRSXUJHWKHRLOLVDWWKHHQGRIWKH ZRUNLQJGD\DIWHU\RXIOXVKWKH$3,SUREHVSUD\VKLHOGDQG KHDWHGFDSLOODU\ 7RSXUJHWKHRLOLQWKHURWDU\YDQHSXPSSURFHHGDVIROORZV 7XUQRIIWKHIORZRIVDPSOHVROXWLRQIURPWKH/&WRWKH06 GHWHFWRU )URPWKH7XQH3OXVZLQGRZFKRRVHControl StandbyRUFOLFN RQWKH2Q6WDQGE\EXWWRQWRSXWWKH06GHWHFWRULQ6WDQGE\ :LWKGUDZWKH$3,IODQJHIURPWKHVSUD\VKLHOGDQGSODFHD VHSWXPRYHUWKHHQWUDQFHWRWKHKHDWHGFDSLOODU\ 2SHQWKHJDVEDOODVWYDOYHRQWKHURWDU\YDQHSXPSE\WXUQLQJ LWWRSRVLWLRQ 5HIHUWRWKHPDQXDOWKDWFDPHZLWKWKHSXPS IRUWKHORFDWLRQRIWKHJDVEDOODVWYDOYH $OORZWKHSXPSWRUXQIRUKRXUVZLWKWKHJDVEDOODVWYDOYH RSHQ $IWHUKRXUVFORVHWKHJDVEDOODVWYDOYHE\WXUQLQJLWWR SRVLWLRQ Finnigan: LCQ MS Detector Hardware Manual

208 MS DETECTOR MAINTENANCE Replacing the Oil Reservoir in the Turbomolecular Pump 5.7 Replacing the Oil Reservoir in the Turbomolecular Pump <RXQHHGWRUHSODFHWKHRLOUHVHUYRLULQWKHWXUERPROHFXODUSXPS DWOHDVWRQFHD\HDU5HSODFLQJWKHRLOUHVHUYRLULQWKH WXUERPROHFXODUSXPSLQYROYHVWKHIROORZLQJVWHSV 5HPRYLQJWKHWXUERPROHFXODUSXPS 5HSODFLQJWKHWXUERPROHFXODUSXPSRLOUHVHUYRLU 5HLQVWDOOLQJWKHWXUERPROHFXODUSXPS 1RWH,IWKHWXUERPROHFXODUSXPSIDLOVLWPXVWEHUHSODFHG 317RUHSODFHWKHWXUERPROHFXODUSXPSUHPRYH WKHSXPSDVGHVFULEHGLQWKHWRSLF5HPRYLQJWKH 7XUERPROHFXODU3XPS 7KHQLQVWDOODQHZSXPSDVGHVFULEHG LQWKHWRSLF5HLQVWDOOLQJWKH7XUERPROHFXODU3XPS Removing the Turbomolecular Pump 7RUHPRYHWKHWXUERPROHFXODUSXPSSURFHHGDVIROORZV 1. Shut down and vent the system as described in the topic Shutting Down the System Completely in the System Shutdown, Startup, and Reset chapter. :$51,1*0DNHVXUHWKDWWKH/&4SRZHUFRUGLVXQSOXJJHG EHIRUH\RXSURFHHG :$5181*'DV/&4PX YRP1HW]JHWUHQQWZHUGHQEHYRU LUJHQGZHOFKH(LQJULIIHYRUJHQRPQHQZHUGHQ Finnigan: LCQ MS Detector Hardware Manual 5-75

209 MS DETECTOR MAINTENANCE Replacing the Oil Reservoir in the Turbomolecular Pump $9(57,66(0(179HULILH]TXHOHFRUGRQGªDOLPHQWDWLRQGX /&4VRLWGpEUDQFKpGHODSULVHGHFRXUDQWDYDQWGHSURFp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innigan: LCQ MS Detector Hardware Manual

210 MS DETECTOR MAINTENANCE Replacing the Oil Reservoir in the Turbomolecular Pump 5/16-IN. SOCKET SCREW (4 ) CONVECTRO NGAUGE TURBOMOLECULAR PUMP RAILS TURBOMOLECULAR PUMP VACUUM HOSE CLAMP FORELINE UNION POWER CABLE Figure Turbomolecular pump *RRQWRWKHQH[WWRSLF&KDQJLQJWKH7XUERPROHFXODUSXPS 2LO5HVHUYRLU Changing the Turbomolecular Pump Oil Reservoir 7RFKDQJHWKHWXUERPROHFXODUSXPSRLOUHVHUYRLUSURFHHGDV IROORZV :$51,1*7R[LFUHVLGXHVIURPVDPSOHVDUHOLNHO\WREH FRQFHQWUDWHGLQWKHSXPSRLO6SHQWSXPSRLOPXVWEHGLVSRVHGRI LQDFFRUGDQFHZLWKORFDODQGIHGHUDOUHJXODWLRQV Finnigan: LCQ MS Detector Hardware Manual 5-77

211 MS DETECTOR MAINTENANCE Replacing the Oil Reservoir in the Turbomolecular Pump :$5181*'DV3XPSHQgONDQQJLIWLJH3UREHQ5 FNVWlQGH HQWKDOWHQGDKHUPX EHQXW]WHV3XPSHQgOHQWVSUHFKHQGORNDOHU RGHU EHUJHRUGQHWHU%HVWLPPXQJHQHQWVRUJWZHUGHQ $9(57,66(0(17/ªKXLOHGHSRPSHSHXWUHWHQLUGHVUHVLGXV YpQpQHX[2QGRLWGRQFGLVSRVHUGHOªKXLOHGHSRPSH FRQIRUPpPHQWDX[Uq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einstalling the Turbomolecular Pump 7RUHLQVWDOOWKHWXUERPROHFXODUSXPSSURFHHGDVIROORZVVHH )LJXUH 1. Check the condition of the Viton O-rings around the two openings on the bottom of the vacuum manifold. (Use a small flashlight to illuminate the O-rings.) If they have any nicks or breaks, replace them with new ones (P/N ) Finnigan: LCQ MS Detector Hardware Manual

212 MS DETECTOR MAINTENANCE Replacing the Oil Reservoir in the Turbomolecular Pump 2. Place the turbomolecular pump on the turbomolecular pump rails. 3. Slide the turbomolecular pump into position under the openings in the vacuum manifold. 4. With a 5/16-in. ball driver or Allen wrench, carefully tighten the four socket screws that hold the turbomolecular pump to the vacuum manifold. Do not overtighten the screws. 5. Place the centering ring in the end of the vacuum hose. Reconnect the vacuum hose (with the centering ring in place) to the turbomolecular pump. Tighten the red hose clamp (Edwards, KF20) that secures the vacuum hose to the turbomolecular pump. 6. Reconnect the turbomolecular pump power cable. 7. If necessary, reconnect the vacuum hoses to the foreline union. Tighten the clamping rings to secure the vacuum hoses to the foreline union. 8. Reinstall the top cover of the MS detector by following the procedure in the topic Reinstalling the Top Cover of the MS Detector on page Close the left and right front doors of MS detector. 10. Reconnect any tubing between the syringe pump and the API source that you disconnected earlier. 11. Restart the system as described in the topic Starting Up the System After a Complete Shutdown in the System Shutdown, Startup, and Reset chapter. Finnigan: LCQ MS Detector Hardware Manual 5-79

213 MS DETECTOR MAINTENANCE Cleaning the Fan Filter 5.8 Cleaning the Fan Filter <RXQHHGWRFOHDQWKHIDQILOWHUORFDWHGRQWKHUHDURIWKH06 GHWHFWRUHYHU\IRXUPRQWKV7RFOHDQWKHIDQILOWHUSURFHHGDV IROORZV 5HPRYHWKHIDQILOWHUE\UHDFKLQJEHKLQGWKH06GHWHFWRUDQG SXOOLQJWKHIDQILOWHURXWWRWKHULJKW :DVKWKHIDQILOWHULQDVROXWLRQRIVRDSDQGZDWHU 5LQVHWKHIDQILOWHUZLWKWDSZDWHU 6TXHH]HWKHZDWHUIURPWKHIDQILOWHUDQGDOORZLWWRDLUGU\ :KHQWKHIDQILOWHULVFRPSOHWHO\GU\UHLQVWDOOLWRQWKHUHDU RIWKH06GHWHFWRU>RUUHSODFHLWZLWKDQHZRQH 5-80 Finnigan: LCQ MS Detector Hardware Manual

214 SYSTEM SHUTDOWN, STARTUP, AND RESET Contents Contents 6<67(06+87'2:167$5783$1'5(6(7 6KXWWLQJ'RZQWKH6\VWHPLQDQ(PHUJHQF\ 3ODFLQJWKH6\VWHPLQ6WDQGE\&RQGLWLRQ 6KXWWLQJ'RZQWKH6\VWHP&RPSOHWHO\ 6WDUWLQJ8SWKH6\VWHPDIWHUD&RPSOHWH6KXWGRZQ 6WDUWLQJ8SWKH/& 6WDUWLQJ8SWKH'DWD6\VWHP 6WDUWLQJ8SWKH06'HWHFWRU 6WDUWLQJ8SWKH$XWRVDPSOHU 6HWWLQJ8S&RQGLWLRQVIRU2SHUDWLRQ 5HVHWWLQJWKH06'HWHFWRU 5HVHWWLQJWKH7XQHDQG&DOLEUDWLRQ3DUDPHWHUVWRWKHLU'HIDXOW9DOXHV 5HVHWWLQJWKH'DWD6\VWHP 7XUQLQJ2II6HOHFWHG06'HWHFWRU&RPSRQHQWV List of Illustrations )LJXUH3RZHUSDQHOVKRZLQJWKH5HVHWEXWWRQHOHFWURQLFVVHUYLFHVZLWFKDQGWKH PDLQSRZHUFLUFXLWEUHDNHUVZLWFK List of Tables 7DEOH2Q2II6WDWXVRI06'HWHFWRU&RPSRQHQWV9ROWDJHVDQG*DV)ORZV Finnigan: LCQ MS Detector Hardware Manual 6-i

215 SYSTEM SHUTDOWN, STARTUP, AND RESET 6. SYSTEM SHUTDOWN, STARTUP, AND RESET 0DQ\PDLQWHQDQFHSURFHGXUHVIRUWKH/&4V\VWHPUHTXLUHWKDW WKH06GHWHFWRUEHVKXWGRZQFRPSOHWHO\,QDGGLWLRQWKH/&4 FDQEHSODFHGLQ6WDQGE\LIWKHV\VWHPLVQRWWREHXVHGIRU KRXUVRUPRUH In this chapter procedures are provided to do the following: 6KXWGRZQWKHV\VWHPLQDQHPHUJHQF\ 3ODFHWKHV\VWHPLQVWDQGE\FRQGLWLRQ 6KXWGRZQWKHV\VWHPFRPSOHWHO\ 6WDUWXSWKHV\VWHPDIWHUDFRPSOHWHVKXWGRZQ 5HVHWWKH06GHWHFWRU 5HVHWWKHWXQHDQGFDOLEUDWLRQSDUDPHWHUVWRWKHLUGHIDXOW YDOXHV 5HVHWWKHGDWDV\VWHP 7XUQRIIVHOHFWHG06GHWHFWRUFRPSRQHQWV Finnigan: LCQ MS Detector Hardware Manual 6-1

216 SYSTEM SHUTDOWN, STARTUP, AND RESET Shutting Down the System in an Emergency 6.1 Shutting Down the System in an Emergency If you need to turn off the MS detector in an emergency, place the main power circuit breaker switch, located on the power panel on the right side panel of the MS detector (see )LJXUH), in the Off (O) position. This turns off all power to the MS detector, including the vacuum pumps. Although removing power abruptly will not harm any component within the system, this is not the recommended shutdown procedure to follow. Refer to the Shutting Down the System Completely topic, on page 6-5, for the recommended procedure. To turn off the LC, autosampler, and computer in an emergency, use the on/off switches on the LC, autosampler, and computer. RESET BUTTON ELECTRONICS SERVICE SWITCH MAIN POWER CIRCUIT BREAKER SWITCH Figure 6-1. Power panel, showing the Reset button, electronics service switch, and the main power circuit breaker switch 6-2 Finnigan: LCQ MS Detector Hardware Manual

217 SYSTEM SHUTDOWN, STARTUP, AND RESET Placing the System in Standby Condition 6.2 Placing the System in Standby Condition The LCQ system does not need to be shut down completely if you are not going to use it for a short period of time, such as overnight or over weekends. When you are not going to operate the system for 12 hours or more, you can leave the system in a standby condition. Use the following procedure to place the LCQ system in the standby condition: 1. Wait until data acquisition, if any, is complete. 2. Turn off the flow of sample solution from the LC to the API source, as follows: D E In the Tune Plus window, choose Setup LC Pump (or click on the LC button). The LC Pump dialog box appears. Select the 2II RSWLRQEXWWRQ. Then click on OK to stop the LC pump. 1RWH)RULQVWUXFWLRQVRQKRZWRRSHUDWHWKH/&IURPWKHIURQW SDQHOUHIHUWRWKHPDQXDOWKDWFDPHZLWKWKH/& 8. From the Tune Plus window, choose Control Standby RUFOLFN RQWKH2Q6WDQGE\EXWWRQWRSXWWKH06GHWHFWRULQ6WDQGE\. :KHQ\RXFKRRVH&RQWURO_6WDQGE\WKH/&4turns off the electron multiplier, conversion dynode, 8 kv power to the API source, ring electrode RF voltage, and octapole RF voltage. LCQ also turns off the auxiliary gas and sets the sheath gas flow to 20 units. See Table 6-1 on page 6-17 for the On/Off status of MS detector components when the 06GHWHFWRULVLQWKHVWDQGE\FRQGLWLRQ. The System LED on the front panel of the MS detector is illuminated yellow when the system is in Standby. 9. Flush the spray shield and the entrance end of the heated capillary of the API source as describe in the topic Flushing the Spray Shield and Heated Capillary in the MS Detector Maintenance chapter. Cap the heated capillary with the septum. Leave the API flange withdrawn from the spray shield. Finnigan: LCQ MS Detector Hardware Manual 6-3

218 SYSTEM SHUTDOWN, STARTUP, AND RESET Placing the System in Standby Condition 10. Purge the rotary-vane pump oil as described in the topic Purging the Rotary-Vane Pump Oil in the MS Detector Maintenance chapter. /HDYHWKH06GHWHFWRUSRZHURQ /HDYHWKH/&SRZHURQ /HDYHWKHDXWRVDPSOHUSRZHURQ /HDYHWKHGDWDV\VWHPSRZHURQ 6-4 Finnigan: LCQ MS Detector Hardware Manual

219 SYSTEM SHUTDOWN, STARTUP, AND RESET Shutting Down the System Completely 6.3 Shutting Down the System Completely The LCQ system does not need to be shut down completely if you are not going to use it for a short period of time, such as overnight or over weekends. (See the topic Placing the System in Standby Condition, above.) Shut down the system completely only if it is to be unused for an extended period or if it must be shut down for a maintenance or service procedure. Use the following procedure to shut down the LCQ system completely: 1. Turn off the flow of sample solution from the LC (or other sample introduction device). 1RWH)RULQVWUXFWLRQVRQKRZWRRSHUDWHWKH/&IURPWKHIURQW SDQHOUHIHUWRWKHPDQXDOWKDWFDPHZLWKWKH/& 8. From the Tune Plus window, choose Control Standby RUFOLFN RQWKH2Q6WDQGE\EXWWRQWRSXWWKH06GHWHFWRULQ6WDQGE\. :KHQ\RXFKRRVH&RQWURO_6WDQGE\WKH/&4turns off the electron multiplier, conversion dynode, 8 kv power to the API source, main RF voltage, and octapole RF voltage. 9. Place the electronics service switch, located on the power panel (see Figure 6-1 on page 6-2), in the Service (OFF, O) position. Power to the non-vacuum system electronics is turned off when you place the electronics service switch in the Service (OFF, O) position. The LCQ also turns off the sheath gas and auxiliary gas flows to the API source. 3ODFHWKHPDLQSRZHUFLUFXLWEUHDNHUVZLWFKORFDWHGRQWKH SRZHUSDQHO)LJXUHLQWKH2II2SRVLWLRQ:KHQ\RX SODFHWKHPDLQSRZHUFLUFXLWEUHDNHUVZLWFKLQWKH2II2 SRVLWLRQWKHIROORZLQJRFFXUV $OOSRZHUWRWKH06GHWHFWRULQFOXGLQJWKH WXUERPROHFXODUSXPSDQGURWDU\YDQHSXPSLVWXUQHGRII $OO/('VRQWKHIURQWSDQHORIWKH06GHWHFWRUDUHRII 7KHEDWWHU\EDFNXSRQWKH9HQW'HOD\3&%SURYLGHV SRZHUWRWKHYHQWYDOYHIRUV$IWHUVDFLUFXLWRQ WKH9HQW'HOD\3&%WLPHVRXWDQGSRZHUWRWKHYHQW Finnigan: LCQ MS Detector Hardware Manual 6-5

220 SYSTEM SHUTDOWN, STARTUP, AND RESET Shutting Down the System Completely YDOYHVROHQRLGLVVKXWRII:KHQSRZHUWRWKHYHQWYDOYH VROHQRLGLVVKXWRIIWKHYHQWYDOYHRSHQVDQGWKHYDFXXP PDQLIROGLVYHQWHGWRILOWHUHGDLU<RXFDQKHDUDKLVVLQJ VRXQGDVWKHDLUSDVVHVWKURXJKWKHDLUILOWHU $IWHUDERXWPLQWKHYDFXXPPDQLIROGLVDWDWPRVSKHULF SUHVVXUH 11. Unplug the power cord for the MS detector. :$51,1*$OORZKHDWHGFRPSRQHQWVWRFRROEHIRUHVHUYLFLQJ WKHP :$5181*:DUWHQ6LHHUKLW]WH.RPSRQHQWHQHUVWQDFKGHP GLHVHVLFKDEJHN KOWKDEHQ $9(57,66(0(173HUPHWWUHDX[FRPSRVDQWVFKDXIIpVGH UHIURLGLUDYDQWG\HIIHFWXHUWRXWVHUYLFH 1RWH,I\RXDUHSODQQLQJWRSHUIRUPURXWLQHRUSUHYHQWLYHV\VWHP PDLQWHQDQFHRQWKH06GHWHFWRURQO\\RXGRQRWQHHGWRWXUQRII WKH/&GDWDV\VWHPDQGDXWRVDPSOHU,QWKLVFDVHWKHVKXWGRZQ SURFHGXUHLVFRPSOHWHG+RZHYHULI\RXGRQRWSODQWRRSHUDWH \RXUV\VWHPIRUDQH[WHQGHGSHULRGRIWLPHZHUHFRPPHQGWKDW \RXWXUQRIIWKH/&GDWDV\VWHPDQGDXWRVDPSOHUDVGHVFULEHGLQ VWHSVWKURXJKEHORZ 6. Turn off the LC. Follow the procedure described in the manual that came with the LC. 7. Turn off the helium damping gas supply at the tank. 6-6 Finnigan: LCQ MS Detector Hardware Manual

221 SYSTEM SHUTDOWN, STARTUP, AND RESET Shutting Down the System Completely 8. Turn off the nitrogen supply at the tank. If you have Windows NT 3.5 installed on your data system, go to step 10. If you have Windows NT 4.0 installed on your data system, go on to the next step. 9. Turn off the data system with Windows NT 4.0 as follows: a. Click on the Start button and choose Shut Down. The Shut Down Windows dialog box appears. b. Select the Shut down the computer option button, and then click on Yes to start the Windows NT shutdown procedure. c. When the Windows NT shutdown procedure tells you that it is safe to turn off the computer, turn off the monitor and computer by using the on/off switches. Go to step Turn off the data system with Windows NT 3.5 as follows: a. In the Windows NT Program Manager window, choose File Shutdown. The Shutdown Computer dialog box appears. b. Select the Shutdown option, and then click on OK to start the Windows NT shutdown procedure. c. When the Windows NT shutdown procedure tells you that it is safe to turn off the computer, turn off the monitor and computer by using the on/off switches. 11. Turn off the printer by using the on/off switch. 12. Turn off the (optional) autosampler by using the main power on/off switch. Finnigan: LCQ MS Detector Hardware Manual 6-7

222 SYSTEM SHUTDOWN, STARTUP, AND RESET Starting Up the System after a Complete Shutdown 6.4 Starting Up the System after a Complete Shutdown To start up the LCQ system after it has been shut down completely, you need to do the following: 6WDUWXSWKH/& 6WDUWXSWKHGDWDV\VWHP 6WDUWXSWKH06GHWHFWRU 6WDUWXSWKHRSWLRQDODXWRVDPSOHU 6HWXSFRQGLWLRQVIRURSHUDWLRQ Starting Up the LC To start up the LC, follow the startup proceedure described in the manual that came with the LC. If necessary, configure the LC as described in Appendix A: Control of External Devices. Do not turn on the liquid flow to the MS detector Starting Up the Data System Use the following procedure to start up the data system: 1. Turn on the monitor, computer, and printer. 2EVHUYHWKH:LQGRZV17VWDUWXSSURFHGXUHRQWKHPRQLWRU :KHQ\RXDUHSURPSWHGWRVHOHFWWKHRSHUDWLQJV\VWHPWR VWDUWXVHWKHDUURZNH\WRVHOHFWWindows NT Workstation 7KHQSUHVV<Enter> 3. Press <Ctrl>-<Alt>-<Del> when you are prompted to do so. Then, click on OK or enter your password (if you have one) in the Welcome dialog box (Windows NT 3.5) or Logon Information dialog box (Windows NT 4.0) to complete the start up procedure. 6-8 Finnigan: LCQ MS Detector Hardware Manual

223 SYSTEM SHUTDOWN, STARTUP, AND RESET Starting Up the System after a Complete Shutdown Starting Up the MS Detector Use the following procedure to start up the MS detector. 1RWH7KHGDWDV\VWHPPXVWEHUXQQLQJEHIRUH\RXVWDUWXSWKH 06GHWHFWRU7KH06GHWHFWRUZLOOQRWRSHUDWHXQWLOVRIWZDUHLV UHFHLYHGIURPWKHGDWDV\VWHP 1. Turn on the flows of helium and nitrogen at the tanks if they are off. 2. Make sure that the main power circuit breaker switch is in the Off (O) position and the electronics service switch is in the Service (OFF, O) position. 3. Plug in the power cord for the MS detector. 4. Place the main power circuit breaker switch in the On ( ) position. When you place the main power circuit breaker switch in the On ( ) position, the rotary-vane pump and the turbomolecular pump are started. All LEDs on the MS detector front panel are off. 5. Place the electronics service switch in the Normal (ON, ) position. When you place the electronics service switch in the Normal (ON, ) position, the following occurs: The Power LED on the MS detector front panel is illuminated green to indicate that power is provided to the MS detector electronics. (The electron multiplier, conversion dynode, 8 kv power to the API source, main RF voltage, and octapole RF voltage remain off.) The embedded computer reboots. After several seconds the Communication LED on the front panel is illuminated yellow to indicate that the data system and the MS detector have started to establish a communication link. After several more seconds, the Communication LED is illuminated green to indicate that the data system and the MS detector have established a communication link. Software for the operation of the MS detector is then transferred from the data system to the MS detector. Finnigan: LCQ MS Detector Hardware Manual 6-9

224 SYSTEM SHUTDOWN, STARTUP, AND RESET Starting Up the System after a Complete Shutdown After 4 or 5 minutes, the System LED is illuminated yellow to indicate that the software transfer from the data system to the MS detector is complete and that the instrument is in Standby. 1RWH7KH9DFXXP/('RQWKHIURQWSDQHORIWKH06GHWHFWRULV LOOXPLQDWHGJUHHQRQO\LIWKHSUHVVXUHLQWKHYDFXXPPDQLIROGLV EHORZWKHPD[LPXPDOORZDEOHSUHVVXUH 7RUULQWKH DQDO\]HUUHJLRQDQG7RUULQWKHFDSLOODU\VNLPPHUUHJLRQDQG WKHVDIHW\LQWHUORFNVZLWFKRQWKH$3,VRXUFHLVGHSUHVVHGWKDWLV WKH$3,IODQJHLVVHFXUHGWRWKHVSUD\VKLHOG If you have an autosampler, go on to the next topic: Starting Up the Autosampler. If you do not have an autosampler, go to the topic: Setting Up Conditions for Operation Starting Up the Autosampler To start up the autosampler, place the main power switch on the autosampler in the on position.,iqhfhvvdu\frqiljxuhwkh DXWRVDPSOHUDVGHVFULEHGLQ$SSHQGL[$&RQWURORI([WHUQDO 'HYLFHVFor procedures for placing sample vials, preparing solvent and waste bottles, installing syringes, etc., refer to the manual that came with the autosampler Setting Up Conditions for Operation <RXQHHGWRGRWKHIROORZLQJWRVHWXS\RXU/&4IRURSHUDWLRQ %HIRUH\RXEHJLQGDWDDFTXLVLWLRQZLWK\RXU/&4V\VWHP\RX QHHGWRDOORZWKHV\VWHPWRSXPSGRZQIRUDWOHDVWKRXU 2SHUDWLRQRIWKHV\VWHPZLWKH[FHVVLYHDLUDQGZDWHULQWKH YDFXXPPDQLIROGFDQFDXVHUHGXFHGVHQVLWLYLW\WXQLQJ SUREOHPVDQGUHGXFHGOLIHWLPHRIWKHHOHFWURQPXOWLSOLHU (QVXUHWKDWWKHKHOLXPSUHVVXUHDQGQLWURJHQSUHVVXUHDUH ZLWKLQWKHRSHUDWLRQDOOLPLWVKHOLXP±SVLJ >±N3D@QLWURJHQ±SVLJ>±N3D@ /RRNDWWKHVWDWXVSDQHOLQWKH7XQH3OXVZLQGRZ&KHFNWR VHHLIWKHSUHVVXUHPHDVXUHGE\WKHLRQJDXJHLVEHORZDERXW 6-10 Finnigan: LCQ MS Detector Hardware Manual

225 SYSTEM SHUTDOWN, STARTUP, AND RESET Starting Up the System after a Complete Shutdown 7RUUDQGWKHSUHVVXUHPHDVXHGE\WKH&RQYHFWURQ JDXJHLVDURXQG7RUU&RPSDUHWKHYDOXHVRIWKHRWKHU SDUDPHWHUVLQWKHVWDWXVSDQHOZLWKYDOXHVWKDW\RXUHFRUGHG SUHYLRXVO\ 6HWXSIRU(6,RU$3&,RSHUDWLRQDVGHVFULEHGLQWKH/&4 2SHUDWRUªV0DQXDO 1RWH<RXGRQRWQHHGWRFDOLEUDWHRUWXQHWKH/&4HDFKWLPH\RX UHVWDUWWKH/&4 &DOLEUDWLRQSDUDPHWHUVDUHLQVWUXPHQWSDUDPHWHUVZKRVH YDOXHVGRQRWYDU\ZLWKWKHW\SHRIH[SHULPHQW<RXQHHGWR FDOLEUDWHWKH/&4SHUKDSVRQFHDPRQWKDQGFKHFNWKHFDOLEUDWLRQ RQFHDZHHN5HIHUWRWKH/&42SHUDWRUªV0DQXDOIRUD SURFHGXUHIRUFDOLEUDWLQJWKH/&4 7XQHSDUDPHWHUVDUHLQVWUXPHQWSDUDPHWHUVZKRVHYDOXHVYDU\ ZLWKWKHW\SHRIH[SHULPHQW<RXQHHGWRWXQHWKH/&4RUFKDQJH WKH7XQH0HWKRGZKHQHYHU\RXFKDQJHWKHW\SHRIH[SHULPHQW 5HIHUWRWKH/&42SHUDWRUªV0DQXDOIRUSURFHGXUHVIRUWXQLQJ WKH/&4LQWKH(6,RU$3&,PRGH1RWHWKDW/&4FRPHVZLWK VHYHUDOVWDQGDUG7XQH0HWKRGVVSHFLILFIRUYDULRXVH[SHULPHQWDO FRQGLWLRQVVRWKDWWXQLQJLVRIWHQQRWUHTXLUHGIRUPDQ\W\SHVRI H[SHULPHQWV Finnigan: LCQ MS Detector Hardware Manual 6-11

226 SYSTEM SHUTDOWN, STARTUP, AND RESET Resetting the MS Detector 6.5 Resetting the MS Detector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fter several seconds, the Communication LED is illuminated yellow to indicate that the data system and the MS detector are starting to establish a communication link. After several more seconds, the Communication LED is illuminated green to indicate that the data system and the MS detector have established a communication link. Software for the operation of the MS detector is then transferred from the data system to the MS detector. After 4 to 5 min the software transfer is complete. The System LED is illuminated either green to indicate that the instrument is functional and the high voltages are on, or yellow to indicate that the instrument is functional and it is in Standby Finnigan: LCQ MS Detector Hardware Manual

227 SYSTEM SHUTDOWN, STARTUP, AND RESET Resetting the Tune and Calibration Parameters to their Default Values 6.6 Resetting the Tune and Calibration Parameters to their Default Values <RXFDQUHVHWWKH/&4WXQHDQGFDOLEUDWLRQSDUDPHWHUVWRWKHLU GHIDXOWYDOXHVDWDQ\WLPH7KLVIHDWXUHPD\EHXVHIXOLI\RXKDYH PDQXDOO\VHWVRPHSDUDPHWHUVWKDWKDYHUHVXOWHGLQOHVVWKDQ RSWLPXPSHUIRUPDQFH7RUHVHWWKH/&4WXQHDQGFDOLEUDWLRQ SDUDPHWHUVWRWKHLUGHIDXOWYDOXHVSURFHHGDVIROORZV 1RWH0DNHVXUHWKDWWKHSUREOHPVWKDW\RXDUHH[SHULHQFLQJDUH QRWGXHWRLPSURSHU$3,VRXUFHVHWWLQJVVSUD\YROWDJHVKHDWKDQG DX[LOLDU\JDVIORZKHDWHGFDSLOODU\WHPSHUDWXUHHWFEHIRUH UHVHWWLQJWKHV\VWHPSDUDPHWHUVWRWKHLUGHIDXOWYDOXHV 1.,QWKH7XQH3OXVZLQGRZFKRRVHFile Restore Factory Calibration WRUHVWRUHWKHGHIDXOWFDOLEUDWLRQSDUDPHWHUVRU FKRRVHFile Restore Factory Tune MethodWRUHVWRUHWKH GHIDXOWWXQHSDUDPHWHUV 2. To optimize the LCQ system parameters (that is, to calibrate or tune the system), perform the calibration or tune procedure as described in the LCQ Operator s Manual. Finnigan: LCQ MS Detector Hardware Manual 6-13

228 SYSTEM SHUTDOWN, STARTUP, AND RESET Resetting the Data System 6.7 Resetting the Data System 7KHUHDUHWZRZD\VWRUHVHWWKHGDWDV\VWHP %\XVLQJWKH:LQGRZV17VKXWGRZQDQGUHVWDUWSURFHGXUH %\SUHVVLQJWKHUHVHWEXWWRQRQWKHSHUVRQDOFRPSXWHU,ISRVVLEOHXVHWKH:LQGRZV17RUVKXWGRZQDQGUHVWDUW SURFHGXUHWRVKXWGRZQDQGUHVWDUWWKHGDWDV\VWHPVRWKDW :LQGRZV17FDQSURSHUO\FORVHDSSOLFDWLRQVDQGVDYHFKDQJHVWR ILOHV 7RUHVHWWKHGDWDV\VWHPE\XVLQJWKH:LQGRZV17VKXWGRZQ DQGUHVWDUWSURFHGXUHSURFHHGDVIROORZV 1. In the Windows NT Program Manager window, choose File Shutdown. The Shutdown Computer dialog box appears. 2. Select the 6KXWGRZQDQG5HVWDUW option, and then click on OK to start the Windows NT shutdown and restart procedure. 2EVHUYHWKH:LQGRZV17VKXWGRZQDQGUHVWDUWSURFHGXUHRQ WKHPRQLWRU:KHQ\RXDUHSURPSWHGWRVHOHFWWKHRSHUDWLQJ V\VWHPWRVWDUWXVHWKHDUURZNH\WRVHOHFW:LQGRZV17 :RUNVWDWLRQ7KHQSUHVV<Enter> 4. Press <Ctrl>-<Alt>-<Del> when you are prompted to do so. Then, click on OK or enter your password (if you have one) in the Welcome dialog box to complete the shutdown and restart procedure. 7RUHVHWWKHGDWDV\VWHPE\XVLQJWKH:LQGRZV17VKXWGRZQ DQGUHVWDUWSURFHGXUHSURFHHGDVIROORZV 1. Click on the Start button and choose Shut Down. The Shut Down Windows dialog box appears. 2. Select the Restart the computer option button, and then click on Yes to start the Windows NT shutdown and restart procedure. 2EVHUYHWKH:LQGRZV17VKXWGRZQDQGUHVWDUWSURFHGXUHRQ WKHPRQLWRU:KHQ\RXDUHSURPSWHGWRVHOHFWWKHRSHUDWLQJ V\VWHPWRVWDUWXVHWKHDUURZNH\WRVHOHFW:LQGRZV17 :RUNVWDWLRQ7KHQSUHVV<Enter> 6-14 Finnigan: LCQ MS Detector Hardware Manual

229 SYSTEM SHUTDOWN, STARTUP, AND RESET Resetting the Data System 4. Press <Ctrl>-<Alt>-<Del> when you are prompted to do so. Then, click on OK or enter your password (if you have one) in the Logon Information dialog box to complete the shutdown and restart procedure. 1RWH7KHFRPPXQLFDWLRQVOLQNEHWZHHQWKHGDWDV\VWHPDQGWKH 06GHWHFWRUVKRXOGEHDXWRPDWLFDOO\UHVWDEOLVKHGDIWHU\RXUHVHW WKHGDWDV\VWHP:KHQWKLVRFFXUVWKH&RPPXQLFDWLRQ/('RQWKH IURQWSDQHORIWKH06GHWHFWRULVLOOXPLQDWHG\HOORZDQGWKHQ JUHHQ,IWKHV\VWHPLVXQDEOHWRUHVWDEOLVKWKHFRPPXQLFDWLRQV OLQNSUHVVWKH5HVHWEXWWRQRQWKHSRZHUSDQHORIWKH06GHWHFWRU,I\RXDUHXQDEOHWRUHVHWWKHGDWDV\VWHPE\XVLQJWKH:LQGRZV 17VKXWGRZQDQGUHVWDUWSURFHGXUHSURFHHGDVIROORZV 1. Press the reset button on the personal computer. 2EVHUYHWKH:LQGRZV17VKXWGRZQDQGUHVWDUWSURFHGXUHRQ WKHPRQLWRU:KHQ\RXDUHSURPSWHGWRVHOHFWWKHRSHUDWLQJ V\VWHPWRVWDUWXVHWKHDUURZNH\WRVHOHFW:LQGRZV17 :RUNVWDWLRQ7KHQSUHVV<Enter> 3. Press <Ctrl>-<Alt>-<Del> when you are prompted to do so. Then, click on OK or enter your password (if you have one) in the Welcome dialog box (Windows NT 3.5) or Logon Information dialog box (Windows NT 4.0) to complete the shutdown and restart procedure. 4. When the shutdown and restart procedure has completed, select Navigator in the LCQ group to display the Navigator window. 1RWH7KHFRPPXQLFDWLRQVOLQNEHWZHHQWKHGDWDV\VWHPDQGWKH 06GHWHFWRUVKRXOGEHDXWRPDWLFDOO\UHVWDEOLVKHGDIWHU\RXUHVHW WKHGDWDV\VWHP:KHQWKLVRFFXUVWKH&RPPXQLFDWLRQ/('RQWKH IURQWSDQHORIWKH06GHWHFWRULVLOOXPLQDWHG\HOORZDQGWKHQ JUHHQ,IWKHV\VWHPLVXQDEOHWRUHVWDEOLVKWKHFRPPXQLFDWLRQV OLQNSUHVVWKHUHVHWEXWWRQRQWKHSRZHUSDQHORIWKH06GHWHFWRU Finnigan: LCQ MS Detector Hardware Manual 6-15

230 SYSTEM SHUTDOWN, STARTUP, AND RESET Turning Off Selected MS Detector Components 6.8 Turning Off Selected MS Detector Components 7KHUHDUHILYHZD\VWKDW\RXFDQWXUQRIIVRPHRUDOORIWKH06 GHWHFWRUFRPSRQHQWV 7XUQRIILQGLYLGXDO06GHWHFWRUFRPSRQHQWVIURPWKH7XQH 3OXVZLQGRZ7XUQLQJRIILQGLYLGXDO06GHWHFWRUFRPSRQHQWV PD\EHQHFHVVDU\ZKHQWURXEOHVKRRWLQJRUZKHQUXQQLQJ FHUWDLQGLDJQRVWLFSURFHGXUHV 3ODFHWKH06GHWHFWRULQ6WDQGE\6WDQGE\LVWKHQRUPDO FRQGLWLRQWROHDYHWKH06GHWHFWRULQZKHQLWLVQRWLQXVH &KRRVHControl Standby RUWRJJOHWKH2Q6WDQGE\EXWWRQ IURPWKH7XQH3OXVZLQGRZWRSODFHWKH06GHWHFWRULQ 6WDQGE\. $OWHUQDWLYHO\\RXFDQFKRRVHInstrument System StandbyIURPWKH1DYLJDWRUZLQGRZ 3ODFHWKH06GHWHFWRULQWKH2IIFRQGLWLRQ7KH2IIFRQGLWLRQ LVVLPLODUWR6WDQGE\H[FHSWDOOKLJKYROWDJHFRPSRQHQWVRIWKH 06GHWHFWRUDUHWXUQHGRII&KRRVHControl Off IURPWKH 7XQH3OXVZLQGRZWRSODFHWKH06GHWHFWRULQWKH2II FRQGLWLRQ.$OWHUQDWLYHO\\RXFDQFKRRVHInstrument System OffIURPWKH1DYLJDWRUZLQGRZ 3ODFHWKHHOHFWURQLFVVHUYLFHVZLWFKLQWKH6HUYLFH2))2 SRVLWLRQ7KHHOHFWURQLFVVHUYLFHVZLWFKDOORZV\RXWRSHUIRUP PDLQWHQDQFHSURFHGXUHVLQYROYLQJQRQYDFXXPV\VWHP FRPSRQHQWVRIWKH06GHWHFWRU 3ODFHWKHPDLQSRZHUFLUFXLWEUHDNHUVZLWFKLQWKH2II2 SRVLWLRQ3ODFLQJWKHPDLQSRZHUFLUFXLWEUHDNHUVZLWFKLQWKH 2II2SRVLWLRQUHPRYHVDOOSRZHUWRWKH06GHWHFWRU LQFOXGLQJWKHYDFXXPV\VWHP 7KHRQRIIVWDWXVRI06GHWHFWRUFRPSRQHQWVYROWDJHVDQGJDV IORZVLVVXPPDUL]HGLQ7DEOH 6-16 Finnigan: LCQ MS Detector Hardware Manual

231 SYSTEM SHUTDOWN, STARTUP, AND RESET Turning Off Selected MS Detector Components Table 6-1. On/Off Status of MS Detector Components, Voltages, and Gas Flows MS detector component Standby Off Electronics service switch in Service (OFF,O) position Main power circuit breaker switch in Off (O) position Electron multiplier Off Off Off Off Conversion dynode Off Off Off Off Mass analyzer RF/waveform voltages Mass analyzer dc offset voltage Off Off Off Off On Off Off Off Octapole RF voltage Off Off Off Off Octapole dc offset voltage On Off Off Off Interoctapole lens On Off Off Off Tube lens On Off Off Off Heated capillary heater On On Off Off Heated capillary dc offset On Off Off Off Corona discharge needle Off Off Off Off APCI vaporizer Off Off Off Off ESI needle Off Off Off Off Sheath gas On (20 units) Off Off Off Auxiliary gas Off Off Off Off Helium damping gas On On On On Vent valve Closed Closed Closed Open (after 30 s) Turbomolecular pump On On On Off Rotary-vane pump On On On Off Vent Delay PCB On On On Off (after 30 s) Embedded computer On On Off Off Turbomolecular Pump Controller Power supply, electron multiplier Power supply, conversion dynode On On On Off Off Off Off Off Off Off Off Off Finnigan: LCQ MS Detector Hardware Manual 6-17

232 SYSTEM SHUTDOWN, STARTUP, AND RESET Turning Off Selected MS Detector Components MS detector component Standby Off Electronics service switch in Service (OFF,O) position Main power circuit breaker switch in Off (O) position Power supply, 8 kv Off Off Off Off Power supply, +5, ±15, +24 V dc switching Power supply, +36, -28 V dc switching Power supply, +24 V dc keep alive On On Off Off On On Off Off On On On Off Power supply, +180 V dc On On Off Off Power supply, ±130 V dc On Off Off Off Power supply, +36 V dc On Off Off Off Power supply, ±20 V dc On On Off Off Power supply, ±215 V dc On Off Off Off Power supply, 24 V ac On On Off Off Power supply, 4 V ac On On Off Off Power supply, ±330 V dc On Off Off Off Fan, turbomolecular pump On On On Off Fan, RF coil On On Off Off Fans, tower On On Off Off Fan, embedded computer On On Off Off Convectron gauge On On Off Off Ion gauge On On Off Off Syringe pump Off Off Off Off 6-18 Finnigan: LCQ MS Detector Hardware Manual

233 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Contents Contents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ist of Illustrations )LJXUH'LDJQRVWLFVGLDORJER[ )LJXUH6\VWHP&RQWURO3&%VKRZLQJWKHORFDWLRQRIWKHIXVHV )LJXUH7RZHUZLWKWKHHPEHGGHGFRPSXWHUUHSRVLWLRQHG )LJXUH(PEHGGHGFRPSXWHUZLWKIURQWFRYHUUHPRYHG )LJXUH3&%VDQGDVVHPEOLHVWKDWDUHDFFHVVDEOHIURPWKHWRSRIWKH06GHWHFWRU )LJXUH/HIWVLGHRIWKH06GHWHFWRUVKRZLQJWKH5)9ROWDJH&RQWURO3&% )LJXUH5HDUYLHZRIWKH06GHWHFWRUVKRZLQJWKH9HQW'HOD\3&%EDWWHU\ EDFNXSDQG/RZ3DVV)LOWHU3&% List of Tables 7DEOH06'HWHFWRU)XVHV Finnigan: LCQ MS Detector Hardware Manual 7-i

234 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT 7. DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT 0DQ\RIWKH06GHWHFWRUFRPSRQHQWVFDQEHWHVWHGE\WKH/&4 GLDJQRVWLFV)LQQLJDQªVVHUYLFHSKLORVRSK\IRUWKH/&4V\VWHP FDOOVIRUWURXEOHVKRRWLQJWRWKHORZHVWSDUWDVVHPEO\3&%RU PRGXOHOLVWHGLQWKH5HSODFHDEOH3DUWVFKDSWHU<RXVKRXOG UHSODFH/&4FRPSRQHQWVZKHQLQGLFDWHGE\WKH/&4GLDJQRVWLFV E\)LQQLJDQ7HFKQLFDO6XSSRUWRUE\D)LQQLJDQ&XVWRPHU 6XSSRUW(QJLQHHU 7KLVFKDSWHUFRYHUVWKHIROORZLQJWRSLFV 5XQQLQJWKH/&4GLDJQRVWLFV 5HSODFLQJDIXVHLQWKH06GHWHFWRU 5HSODFLQJD3&%RUDVVHPEO\LQWKH06GHWHFWRU Finnigan: LCQ MS Detector Hardware Manual 7-1

235 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Running the LCQ Diagnostics 7.1 Running the LCQ Diagnostics 7KH/&4GLDJQRVWLFVLVXVHGWRWHVWWKHPDMRUHOHFWURQLFFLUFXLWV ZLWKLQWKHLQVWUXPHQWDQGLQGLFDWHZKHWKHUWKHFLUFXLWVSDVVRU IDLOWKHWHVWV,IWKHUHLVDSUREOHPZLWKWKHLQVWUXPHQW HOHFWURQLFVWKH/&4GLDJQRVWLFVFDQRIWHQORFDWHWKHSUREOHP<RX FDQWKHQRIWHQFRUUHFWWKHSUREOHP\RXUVHOIE\UHSODFLQJDIDXOW\ 3&%RUDVVHPEO\ The /&4GLDJQRVWLFVdoes not diagnose problems that are not electrical in nature. For example, it does not diagnose poor sensitivity due to misaligned or dirty components or to improper tuning. Therefore, it is important to have someone who is familiar with system operation and basic hardware theory run the diagnostics and use it to assist in isolating any problems. Before running the diagnostics, you should consider the following: 'LGWKHV\VWHPIDLOZKHQ\RXZHUHUXQQLQJVDPSOHV" 'LGSUREOHPVRFFXUDIWHU\RXSHUIRUPHGPDLQWHQDQFHRQWKH LQVWUXPHQWGDWDV\VWHPRUSHULSKHUDOV" 'LG\RXFKDQJHWKHV\VWHPªVFRQILJXUDWLRQFDEOHVRU SHULSKHUDOVMXVWEHIRUHWKHSUREOHPRFFXUUHG" If the answer is yes to the first item above, there is the possibility of a hardware failure, and running the diagnostics is appropriate. If the answer is yes to one of the last two items above, the problem is probably mechanical, not electrical. Reverify that alignment, configurations, and cable connections are correct before you run the /&4GLDJQRVWLFV. To run the /&4GLDJQRVWLFVSURFHHGDVIROORZV 7XQHWKHULQJHOHFWURGHDQGRFWDSROH5)YROWDJHVDVGHVFULEHG LQWKHWRSLF7XQLQJWKH5LQJ(OHFWURGHDQG2FWDSROH5) 9ROWDJHVLQWKH06'HWHFWRU0DLQWHQDQFHFKDSWHU,QWKH7XQH3OXVZLQGRZFKRRVHDiagnostics Diagnostics 7KH'LDJQRVWLFVGLDORJER[DSSHDUV6HH)LJXUH 6HOHFWRQHRIWKHIROORZLQJRSWLRQV To test all of the electronic subsystems (that is, the vacuum system, power supplies, lenses, ion detection system, and 7-2 Finnigan: LCQ MS Detector Hardware Manual

236 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Running the LCQ Diagnostics RF voltage electronics), click on the All tab and select the Everything option. To test an individual subsystem, click on the tab corresponding to that subsystem and select the appropriate options. 4. Select how many times you want to run the tests, and whether or not you want to print reports or to stop on a failure. 5. Click on the Start button to start the diagnostics. LCQ starts testing and displays a chronological log of all diagnostic tests in the Testing text box. Once testing for a specific subsystem is completed, LCQ displays either Pass or Fail in the Results group box. If the LCQ diagnostics indicates a problem, perform thepdlqwhqdqfh SURFHGXUHLQGLFDWHGE\WKH/&4GLDJQRVWLFVE\)LQQLJDQ 7HFKQLFDO6XSSRUWRUE\D)LQQLJDQ&XVWRPHU6XSSRUW(QJLQHHU )RUPRUHLQIRUPDWLRQRQWKH/&4GLDJQRVWLFVUHIHUWRWKH/&4 RQOLQH+HOS Figure 7-1. Diagnostics dialog box Finnigan: LCQ MS Detector Hardware Manual 7-3

237 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing a Fuse 7.2 Replacing a Fuse Fuses protect the various circuits by opening the circuits whenever overcurrent occurs. On the MS detector, most of the fuses are located on the System Control PCB. Several fuses, however, are located on the RF Voltage Amplifier PCB, Analyzer Auxiliary PCB, and Power Module. The function and current rating of the various fuses are listed in Table 7-1. &KHFNIXVHVZKHQSRZHULVORVWWRDIXVHGVXEV\VWHP :$51,1*$OZD\VSODFHWKHHOHFWURQLFVVHUYLFHVZLWFKLQWKH 6HUYLFH2))2SRVLWLRQRUVKXWGRZQWKHV\VWHPDQGGLVFRQQHFW WKHSRZHUFRUGEHIRUH\RXUHSODFHIXVHVon the System Control PCB, RF Voltage Amplifier PCB, or Analyzer Auxiliary PCB :$5181*6FKDOWHQ6LHVWHWVGHQ+DXSWVFKDOWHUI U (OHNWURQLNWHLOHDXI:DUWXQJ6HUYLFH2))2RGHUVFKDOWHQ6LH GDV6\VWHPDEXQG]LHKHQ6LHGHQ1HW]VWHFNHUDEEHYRU6LHHLQH 6LFKHUXQJDQGHU)ODFKEDXJUXSSHGHV6\VWHPSXOWHVGHV5) 9HUVWlUNHUVRGHUGHV+LOIVDQDO\VHJHUlWHVDXVZHFKVHOQ $9(57,66(0(173ODFHUWRXMRXUVOHGLVMRQFWHXUGH EUDQFKHPHQWHQSRVLWLRQ6HUYLFH2))2RXPHWWUHOHV\VWqPH KRUVWHQVLRQHWGpFRQQHFWHUOHFRUGRQGDOLPHQWDWLRQDYDQWGH UHPSODFHUXQIXVLEOHVXUOH3&%&RQWU{OHGHV\VWqPH3&% $PSOLILFDWHXU5)RX3&%$QDO\VHXUDX[LOLDLUH 7-4 Finnigan: LCQ MS Detector Hardware Manual

238 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing a Fuse Table 7-1. MS Detector Fuses Location Fus e Circuit Description P/N System Control PCB System Control PCB System Control PCB System Control PCB System Control PCB System Control PCB System Control PCB System Control PCB System Control PCB System Control PCB RF Voltage Amplifier PCB RF Voltage Amplifier PCB Analyzer Auxiliary PCB Analyzer Auxiliary PCB F1 F2 F3 F4 Octapoles and tube lens power supplies, ion gauge grid Octapoles and tube lens power supplies Heated capillary, octapoles, and mass analyzer DC offsets Heated capillary, octapoles, and mass analyzer DC offsets 0.16 A, time lag, 5 x 20 mm, 250 V 0.16 A, time lag, 5 x 20 mm, 250 V 0.16 A, time lag, 5 x 20 mm, 250 V 0.16 A, time lag, 5 x 20 mm, 250 V F5 Ion gauge filament 3.15 A, time lag, 5 x 20 mm, 250 V F6 F7 F8 F9 F10 Heated capillary heater Conversion dynode power supply RF detector power supply RF detector power supply APCI vaporizer heater Power Module F1 Voltage select switch 2.5 A, Type F, 5 x 20 mm, 250 V 0.25 A, time lag, 5 x 20 mm, 250 V 0.4 A, time lag, 5 x 20 mm, 250 V 0.4 A, time lag, 5 x 20 mm, 250 V 2.5 A, time lag, 5 x 20 mm, 250 V F1 +36 V 1.0 A, quick act, 5 x 20 mm, 250 V F2-28 V 0.5 A, quick act, 5 x 20 mm, 250 V F1-28 V 1.6 A, quick act, 5 x 20 mm, 250 V F2 +36 V 1.6 A, quick act, 5 x 20 mm, 250 V 3.5 A, time lag, 5 x 20 mm, 250 V Finnigan: LCQ MS Detector Hardware Manual 7-5

239 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing a Fuse &DXWLRQ8VHRQO\WKHIXVHVVSHFLILHGLQ7DEOH1HYHUUHSODFH DIXVHZLWKDIXVHRIDGLIIHUHQWW\SHYROWDJHRUFXUUHQWUDWLQJ 1RWH7RUHSODFHWKHIXVHLQWKH3RZHU0RGXOH\RXQHHGWRUHPRYH WKHWRZHU6\VWHP&RQWURO3&%DQGHPEHGGHGFRPSXWHU'RQRW UHSODFHWKHIXVHLQWKH3RZHU0RGXOHXQOHVV\RXDUHTXDOLILHGWRGR VR 7RUHSODFHDIXVHRQWKH6\VWHP&RQWURO3&%5)9ROWDJH $PSOLILHU3&%RU$QDO\]HU$X[LOLDU\3&%SURFHHGDVIROORZV 1. Place the electronics service switch in the Service (OFF, O) position (or shut down and vent the LCQ system as described in the topic Shutting Down the System Completely in the System Shutdown, Startup, and Reset chapter). :$51,1*0DNHVXUHWKDWWKH/&4HOHFWURQLFVHUYLFHVZLWFKLV LQWKH6HUYLFH2))2SRVLWLRQRUVKXWGRZQWKHV\VWHPDQG GLVFRQQHFWWKHSRZHUFRUGEHIRUH\RXSURFHHG :$5181*.RQWUROOLHUHQ6LHGD GHU+DXSWVFKDOWHUI U/&4 (OHNWURQLNWHLOHDXI:DUWXQJ6HUYLFH2))2JHVFKDOWHWLVWRGHU VFKDOWHQ6LHGDV6\VWHPDEXQG]LHKHQ6LHGHQ1HW]VWHFNHUDE EHYRU6LHIRUWIDKUHQ $9(57,66(0(176DVVXUHUTXHOHGLVMRQFWHXUGHEUDQFKHPHQW /&4HVWHQSRVLWLRQ6HUYLFH2))2RXPHWWUHOHV\VWqPHKRUV WHQVLRQHWGpFRQQHFWHUOHFRUGRQGDOLPHQWDWLRQDYDQWGH FRQWLQXHU 7-6 Finnigan: LCQ MS Detector Hardware Manual

240 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing a Fuse 1. Remove the top cover of the MS detector as described in the topic Removing the Top Cover of the MS Detector in the MS Detector Maintenance chapter. 7RUHSODFHDIXVHRQWKH6\VWHP&RQWURO3&%JRWRVWHS 7RUHSODFHDIXVHRQWKH5)9ROWDJH$PSOLILHU3&%JRWR VWHS 7RUHSODFHDIXVHRQWKH$QDO\]HU$X[LOLDU\3&%JRWR VWHS 2. To replace a fuse on the System Control PCB, proceed as follows. See Figure 7-2 for the location of the System Control PCB and its fuses. a. Remove the right side cover of the MS detector as follows: i. Loosen the fastener that secures the right side cover to the chassis of the MS detector. ii. Slide the side cover back about 1.25 cm (0.5 in.), and then lift it out and away from the MS detector. b. Remove the protective cover on the System Control PCB as follows: i. Insert a finger into the finger hole on the left side of the cover. ii. Pull out on the cover to detach the tabs on the cover from the slots in the MS detector chassis. &DXWLRQ7RSUHYHQWGDPDJHWRWKHHOHFWURQLFVGXHWRHOHFWURVWDWLF GLVFKDUJHDWWDFKDQHOHFWURVWDWLFGLVFKDUJH(6'VWUDSWR\RXU ZULVWEHIRUHFRQWLQXLQJ c. Locate and replace the defective fuse on the System Control PCB with a fuse of the same type, voltage, and current rating. Refer to Table 7-1. Finnigan: LCQ MS Detector Hardware Manual 7-7

241 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing a Fuse FUSES Figure 7-2. System Control PCB, showing the location of the fuses d. Reinstall the protective cover over the System Control PCB as follows: i. Insert the three tabs on the left side of the cover into the corresponding three slots in the MS detector chassis. ii. Swing the right side of the cover toward the chassis until the tabs in the cover insert and lock into the slots in the chassis. H 5HLQVWDOOWKHULJKWVLGHFRYHURIWKH06GHWHFWRUDV IROORZV L ii. 3ODFHWKHFRYHUDJDLQVWWKHULJKWVLGHRIWKH06 GHWHFWRUVXFKWKDWWKHVWXGVRQWKHFRYHULQVHUWLQWR WKHJXLGHVORWVLQWKH06GHWHFWRUFKDVVLV Slide the side cover forward about 1.25 cm (0.5 in.) until the studs on the cover lock in the guide slots. LLL 7LJKWHQE\KDQGWKHIDVWHQHUWKDWVHFXUHVWKHVLGH FRYHUWRWKHFKDVVLVRIWKH06GHWHFWRU 7-8 Finnigan: LCQ MS Detector Hardware Manual

242 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing a Fuse *RWRVWHS 7RUHSODFHDIXVHRQWKH5)9ROWDJH$PSOLILHU3&%SURFHHGDV IROORZV6HH)LJXUHRQSDJHIRUWKHORFDWLRQRIWKH5) 9ROWDJH$PSOLILHU3&% D :LWKD3KLOOLSVVFUHZGULYHUORRVHQWKHHLJKWVFUHZVWKDW KROGWKHPHWDOFRYHUWRWKH5)9ROWDJH$PSOLILHU3&% 5HPRYHWKHFRYHU b. Locate and replace the defective fuse on the RF Voltage Amplifier PCB with a fuse of the same type, voltage, and current rating. Refer to Table 7-1. c. Place the metal cover over the RF Voltage Amplifier PCB. With a Phillips screwdriver, tighten the eight screws that secure the cover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c. Locate and replace the defective fuse on the Analyzer Auxiliary PCB with a fuse of the same type, voltage, and current rating. Refer to Table 7-1. d. Reinstall the protective cover on the Analyzer Auxiliary PCB. With a Phillips screwdriver, tighten the six screws that hold the metal cover to the Analyzer Auxiliary PCB. e. Reconnect the seven cables that connect to the top of the Analyzer Auxiliary PCB. (Three coaxial cables come from the Analyzer PCB, one coaxial cable comes from the Finnigan: LCQ MS Detector Hardware Manual 7-9

243 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing a Fuse Waveform DDS PCB in the embedded computer, one coaxial cable comes from the Waveform Amplifier PCB, one ribbon cable comes from the System Control PCB, and one cable comes from the Dc Ring Filter PCB.) 6. Reinstall the top cover of the MS detector as described in the topic Reinstalling the Top Cover of the MS Detector in the MS Detector Maintenance chapter. 7. Place the electronics service switch in the Normal (ON, ) position. 8. Run the LCQ diagnostics to verify that the system is operational Finnigan: LCQ MS Detector Hardware Manual

244 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing PCBs and Assemblies in the MS Detector 7.3 Replacing PCBs and Assemblies in the MS Detector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lVH9XQWHUEUHFKXQJVIUHLH 6WURPYHUVRUJXQJ:UDVHQNODSSH)ODFKEDXJUXSSH]XU $E]XJVYHU] JHUXQJ9RU:DUWXQJVDUEHLWHQDQGLHVHQ.RPSRQHQWHQPX GDKHUGHU1HW]VFKDOWHUDXI2))2JHVWHOOW XQGGHU1HW]VWHFNHUYRQGHU6WURPYHUVRUJXQJDEJH]RJHQZHUGHQ Finnigan: LCQ MS Detector Hardware Manual 7-11

245 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing PCBs and Assemblies in the MS Detector $9(57,66(0(17/RUVTXHOHGLVMRQFWHXUGHEUDQFKHPHQWHVW HQSRVLWLRQ6HUYLFH2))2ODOLPHQWDWLRQUHVWHSUpVHQWHjOD SRPSHjSDOHWWHVDXFRQWU{OHXUGHODWXUERSRPSHjODWXUER SRPSHDXYHQWLODWHXUGHODWXUERSRPSHDXEORFGHQWUHWLHQ GDOLPHQWDWLRQ9jODYDQQHGpYHQWHWDX3&%'pODLGHYDQQH $LQVLDYDQWGHIIHFWXHUWRXWVHUYLFHVXUFHVFRPSRVDQWVOH GLVMRQFWHXUGDOLPHQWDWLRQSULQFLSDOHGRLWrWUHPLVHQSRVLWLRQ2II 2HWOHFRUGRQGDOLPHQWDWLRQGRLWrWUHGpEUDQFKpGHODSULVHGH FRXUDQW &DXWLRQ1HYHULQVHUWDWHVWSUREHIRUH[DPSOHDQRVFLOORVFRSH SUREHLQWRWKHVRFNHWVRIIHPDOHFDEOHFRQQHFWRUVRQ3&%V7KLV FDQGDPDJHWKHVRFNHWV Replacing PCBs and Assemblies in the Tower 7KHIROORZLQJ3&%VDQGDVVHPEOLHVDUHLQVWDOOHGLQWKHWRZHU6HH )LJXUH 9±99GFDQG99GF VZLWFKLQJSRZHU VXSSOLHV 7XUERPROHFXODU3XPS&RQWUROOHU N9SRZHUVXSSO\ 3RZHU0RGXOH 1RWH7RVHUYLFHWKH3RZHU0RGXOH\RXQHHGWRUHPRYHWKHWRZHU 6\VWHP&RQWURO3&%DQGHPEHGGHGFRPSXWHU'RQRWVHUYLFHWKH 3RZHU0RGXOHXQOHVV\RXDUHTXDOLILHGWRGRVR 7-12 Finnigan: LCQ MS Detector Hardware Manual

246 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing PCBs and Assemblies in the MS Detector +36 V, -28 V DC SWITCHING POWER SUPPLY +5 V, ±15 V, +24 V DC SWITCHING POWER SUPPLY EMBEDDED COMPUTER (REPOSITIONED) 8 KV POWER SUPPLY TURBO PUMP POWER MODULE CONTROLLER Figure 7-3. Tower (with the embedded computer repositioned) Finnigan: LCQ MS Detector Hardware Manual 7-13

247 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing PCBs and Assemblies in the MS Detector 7RUHSODFHDFRPSRQHQWWKDWLVLQWKHWRZHUSURFHHGDVIROORZV 1. Shut down and vent the system as described in the topic Shutting Down the System Completely in the System Shutdown, Startup, and Reset chapter. :$51,1*0DNHVXUHWKDWWKH/&4SRZHUFRUGLVXQSOXJJHG EHIRUH\RXSURFHHG :$5181*'DV/&4PX YRP1HW]JHWUHQQWZHUGHQEHYRU LUJHQGZHOFKH(LQJULIIHYRUJHQRPQHQZHUGHQ $9(57,66(0(179HULILH]TXHOHFRUGRQGªDOLPHQWDWLRQGX /&4VRLWGpEUDQFKpGHODSULVHGHFRXUDQWDYDQWGHSURFpGHU 3. Remove the top cover of the MS detector as described in the topic Removing the Top Cover of the MS Detector in the MS Detector Maintenance chapter. 4. Reposition the embedded computer (to expose the tower) as follows: a. Disconnect the cables that connect to the top of the embedded computer. b. Loosen the six knurled fasteners that secure the embedded computer to the vacuum manifold, base plate, and chassis. c. Lift up the embedded computer a sufficient distance to access the two cables that connect to the bottom of the embedded computer. Disconnect the two cables that connect to the bottom of the embedded computer. d. Lift the embedded computer and reposition it such that the two hooks in the back of the embedded computer box insert 7-14 Finnigan: LCQ MS Detector Hardware Manual

248 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing PCBs and Assemblies in the MS Detector into the two slots in the MS detector chassis. See Figure 7-4 on page 7-20 for the location of the slots. To replace the switching power supply assembly (which includes the +5 V, ±15 V, +24 V dc and +36 V, -28 V dc switching power supplies), go to step 4. 7RUHSODFHWKH7XUERPROHFXODU3XPS&RQWUROOHUJRWR VWHS 7RUHSODFHWKHN9SRZHUVXSSO\JRWRVWHS 5. To replace the switching power supply assembly (which includes the +5 V, ±15 V, +24 V dc and +36 V, -28 V dc switching power supplies), proceed as follows: a. Disconnect the cable to the RF Voltage Amplifier PCB from the connector on the RF Voltage Amplifier PCB. (See Figure 7-5 on page 7-23 for the location of the RF Voltage Amplifier PCB, Analyzer Auxiliary PCB, and Waveform Amplifier PCB.) b. Disconnect the two cables to the Analyzer Auxiliary PCB from the two connectors on the Analyzer Auxiliary PCB. c. Disconnect the cable to the Waveform Amplifier PCB from the connector on the Waveform Amplifier PCB. d. Disconnect the cable that is connected to the fan cable. e. Disconnect the two cables to the Power Module from the two (upper) connectors on the Power Module. f. Disconnect the cable that is connected to the reset button cable. g. Loosen by hand (or with a Phillips screwdriver) the fastener that holds the switching power supply assembly to the tower. h. Remove the switching power supply assembly from the tower. L 8QSDFNWKHQHZVZLWFKLQJSRZHUVXSSO\DVVHPEO\ 315HWDLQWKHSDFNLQJPDWHULDOVVRWKDW \RXFDQSDFNDQGVKLSWKHGHIHFWLYHVZLWFKLQJSRZHU VXSSO\DVVHPEO\WRWKH)LQQLJDQ5HSDLU&HQWHULQ6DQ -RVH%HVXUHWRQRWHWKHDSSDUHQWSUREOHPRU V\PSWRPVRQWKHHQFORVHGIRUPV Finnigan: LCQ MS Detector Hardware Manual 7-15

249 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing PCBs and Assemblies in the MS Detector j. Install the new switching power supply assembly in the space occupied by the old assembly. k. Tighten by hand the fastener that holds the switching power supply assembly to the tower. l. Reconnect the cable that is connected to the reset button cable. m. Reconnect the two cables to the Power Module to the two (upper) connectors on the Power Module. n. Reconnect the cable that is connected to the fan cable. o. Reconnect the cable to the Waveform Amplifier PCB to the connector on the Waveform Amplifier PCB. p. Reconnect the two cables to the Analyzer Auxiliary PCB to the two connectors on the Analyzer Auxiliary PCB. q. Reconnect the cable to the RF Voltage Amplifier PCB to the connector on the RF Voltage Amplifier PCB. Go to step To replace the Turbomolecular Pump Controller, proceed as follows: a. Disconnect from the Turbomolecular Pump Controller the thick cable to the turbomolecular pump. b. Disconnect from the Turbomolecular Pump Controller the thin cable that comes from the Power Module. c. Loosen by hand (or with a Phillips screwdriver) the fastener that holds the Turbomolecular Pump Controller to the tower. d. Remove the Turbomolecular Pump Controller from the tower. H 8QSDFNWKHQHZ7XUERPROHFXODU3XPS&RQWUROOHU 315HWDLQWKHSDFNLQJPDWHULDOVVRWKDW \RXFDQSDFNDQGVKLSWKHGHIHFWLYH7XUERPROHFXODU 3XPS&RQWUROOHUWRWKH)LQQLJDQ5HSDLU&HQWHULQ6DQ -RVH%HVXUHWRQRWHWKHDSSDUHQWSUREOHPRU V\PSWRPVRQWKHHQFORVHGIRUPV f. Install the new Turbomolecular Pump Controller in the space occupied by the old controller Finnigan: LCQ MS Detector Hardware Manual

250 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing PCBs and Assemblies in the MS Detector g. Tighten by hand the fastener that holds the Turbomolecular Pump Controller to the tower. h. Reconnect to the Turbomolecular Pump Controller the thin cable that comes from the Power Module. i. Reconnect to the Turbomolecular Pump Controller the thick cable that goes to the turbomolecular pump. Go to step To replace the 8 kv power supply, proceed as follows: a. Disconnect from the 8 kv power supply the cable that comes from the API panel. b. Disconnect from the rear of the System Control PCB (at J5), the thick cable that goes to the System Control PCB. c. Loosen by hand (or with a Phillips screwdriver) the fastener that holds the 8 kv power supply to the tower. d. Remove the 8 kv power supply from the tower. H 8QSDFNWKHQHZN9SRZHUVXSSO\31 5HWDLQWKHSDFNLQJPDWHULDOVVRWKDW\RXFDQSDFNDQG VKLSWKHGHIHFWLYHN9SRZHUVXSSO\WRWKH)LQQLJDQ 5HSDLU&HQWHULQ6DQ-RVH%HVXUHWRQRWHWKH DSSDUHQWSUREOHPRUV\PSWRPVRQWKHHQFORVHG IRUPV f. Reinstall the new 8 kv power supply in the space occupied by the old power supply. g. Tighten by hand the fastener that holds the 8 kv power supply to the tower. h. Reconnect to the rear of the System Control PCB (at J5), the thick cable that comes from the 8 kv power supply. i. Reconnect to the 8 kv power supply the thin cable that comes from the API panel. Go to step Return the embedded computer to its original position as follows: Finnigan: LCQ MS Detector Hardware Manual 7-17

251 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing PCBs and Assemblies in the MS Detector a. Lift the embedded computer up and away from the MS detector chassis. Lift up the embedded computer a sufficient distance to access the bottom of the embedded computer. b. Reconnect to the bottom of the embedded computer the cable that comes from the switching power supplies. c. Reconnect the fan power cable to the embedded computer fan. Make sure that the plug on the end of the cable contours the fan (that is, the concave side of the plug is against the fan). d. Reposition the embedded computer in its original position in front of the tower. e. Tighten the six knurled fasteners that secure the embedded computer to the vacuum manifold, base plate, and chassis. f. Reconnect the cables that connect to the top of the embedded computer. See Figure 7-4 on page 7-20 and Figure 7-5 on page Reinstall the top cover of the MS detector as described in the topic Reinstalling the Top Cover of the MS Detector in the MS Detector Maintenance chapter. 10. Close the front doors of the MS detector. 11. Restart the system as described in the topic Starting Up the System After a Complete Shutdown in the System Shutdown, Startup, and Reset chapter. 5XQWKH/&4GLDJQRVWLFVWRYHULI\WKDWWKHV\VWHPLV RSHUDWLRQDO Replacing PCBs in the Embedded Computer 7KH(WKHUQHW3&%31,(((,23&% RSWLRQDO31$FTXLVLWLRQ'633&% 31&RQWURO'633&%31 :DYHIRUP''63&%316HULDO,23&% 31DQG&383&%31UHVLGHLQWKH HPEHGGHGFRPSXWHU6HH)LJXUH 7RUHSODFHD3&%LQWKHHPEHGGHGFRPSXWHUSURFHHGDVIROORZV 7-18 Finnigan: LCQ MS Detector Hardware Manual

252 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing PCBs and Assemblies in the MS Detector 1. Place the electronics service switch in the Service (OFF, O) position (or shut down and vent the LCQ system as described in the topic Shutting Down the System Completely in the System Shutdown, Startup, and Reset chapter). :$51,1*0DNHVXUHWKDWWKH/&4HOHFWURQLFVHUYLFHVZLWFKLV LQWKH6HUYLFH2))2SRVLWLRQRUVKXWGRZQRUVKXWGRZQWKH V\VWHPDQGGLVFRQQHFWWKHSRZHUFRUGEHIRUH\RXSURFHHG :$5181*.RQWUROOLHUHQ6LHGD GHU+DXSWVFKDOWHUI U/&4 (OHNWURQLNWHLOHDXI:DUWXQJ6HUYLFH2))2JHVFKDOWHWLVWRGHU VFKDOWHQ6LHGDV6\VWHPDEXQG]LHKHQ6LHGHQ1HW]VWHFNHUDE EHYRU6LHIRUWIDKUHQ $9(57,66(0(176DVVXUHUTXHOHGLVMRQFWHXUGHEUDQFKHPHQW /&4HVWHQSRVLWLRQ6HUYLFH2))2RXPHWWUHOHV\VWqPHKRUV WHQVLRQHWGpFRQQHFWHUOHFRUGRQGDOLPHQWDWLRQDYDQWGH FRQWLQXHU 2SHQWKHIURQWGRRUVRIWKH06GHWHFWRU /RRVHQWKHWZRNQXUOHGIDVWHQHUVWKDWKROGWKHIURQWFRYHUWR WKHHPEHGGHGFRPSXWHU5HPRYHWKHIURQWFRYHURIWKH HPEHGGHGFRPSXWHU /RFDWHWKH3&%\RXZDQWWRUHSODFH6HH)LJXUH 'LVFRQQHFWDOOHOHFWULFDOFDEOHVWRWKH3&%WKDW\RXZDQWWR UHSODFH :LWKD3KLOOLSVVFUHZGULYHUUHPRYHWKHVFUHZWKDWKROGVWKH 3&%WRWKHFDUGFDJH Finnigan: LCQ MS Detector Hardware Manual 7-19

253 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing PCBs and Assemblies in the MS Detector ETHERNET PCB IEEE 488 I/O PCB (OPTIONAL) REPOSITIONING SLOT ACQUISITION DSP CONTROL DSP PCB WAVEFORM DDS PCB SERIAL I/O PCB CPU PCB REPOSITIONING SLOT Figure 7-4. Embedded computer (with front cover removed) &DXWLRQ7RSUHYHQWGDPDJHWRWKHHOHFWURQLFVGXHWRHOHFWURVWDWLF GLVFKDUJHDWWDFKDQHOHFWURVWDWLFGLVFKDUJH(6'VWUDSWR\RXU ZULVWEHIRUHFRQWLQXLQJ 8QVHDWWKH3&%IURPWKHEDFNSODQHDQGSXOOLWRXWRIWKH HPEHGGHGFRPSXWHU 7-20 Finnigan: LCQ MS Detector Hardware Manual

254 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing PCBs and Assemblies in the MS Detector 8QSDFNWKHQHZ3&%5HWDLQWKHSDFNLQJPDWHULDOVVRWKDW \RXFDQSDFNDQGVKLSWKHGHIHFWLYH3&%WRWKH)LQQLJDQ 5HSDLU&HQWHULQ6DQ-RVH%HVXUHWRQRWHWKHDSSDUHQW SUREOHPRUV\PSWRPVRQWKHHQFORVHGIRUPV 6HDWWKHQHZ3&%LQWKHEDFNSODQH :LWKD3KLOOLSVVFUHZGULYHUUHLQVWDOOWKHVFUHZWKDWKROGVWKH 3&%WRWKHFDUGFDJH 5HFRQQHFWDOOHOHFWULFDOFDEOHVWRWKH3&%WKDW\RXUHSODFHG 5HLQVWDOOWKHIURQWFRYHURIWKHHPEHGGHGFRPSXWHU7LJKWHQ E\KDQGWKHWZRNQXUOHGIDVWHQHUVWKDWKROGWKHIURQWFRYHUWR WKHHPEHGGHGFRPSXWHU &ORVHWKHIURQWGRRUVWRWKH06GHWHFWRU 14. Place the electronics service switch in the Normal (ON, ) position. 5XQWKH/&4GLDJQRVWLFVWRYHULI\WKDWWKHV\VWHPLV RSHUDWLRQDO Finnigan: LCQ MS Detector Hardware Manual 7-21

255 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing PCBs and Assemblies in the MS Detector Replacing the Vent Delay PCB, Ion Gauge, and Vent Valve 7KH9HQW'HOD\3&%LRQJDXJHDQGYHQWYDOYHFDQEHDFFHVVHG IURPWKHWRSRIWKH06GHWHFWRU6HH)LJXUH :$51,1*7KH/&4V\VWHPPXVWEHVKXWGRZQDQGWKHSRZHU FRUGXQSOXJJHGEHIRUH\RXVHUYLFHWKHYHQWYDOYH9HQW'HOD\3&% &RQYHFWURQJDXJHRULRQJDXJH :$5181*9RUGHU:DUWXQJDQGHQIROJHQGHQ.RPSRQHQWHQ PX GDV/&46\VWHPDEJHVWHOOWXQGGHU1HW]VWHFNHUDEJH]RJHQ ZHUGHQ:UDVHQNODSSH)ODFKEDXJUXSSH]XU$E]XJVYHU] JHUXQJ &RQYHFWURQRGHU(LVHQPH JHUlW $9(57,66(0(17/HV\VWqPH/&4GRLWrWUHPLVKRUVWHQVLRQHW OHFRUGRQGDOLPHQWDWLRQGpEUDQFKpDYDQWGHIIHFWXHUWRXWVHUYLFH VXUODYDQQHGpYHQWOH3&%'pODLGHYDQQHODMDXJH&RQYHFWURQ RXODMDXJHGLRQLVDWLRQ 7-22 Finnigan: LCQ MS Detector Hardware Manual

256 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing PCBs and Assemblies in the MS Detector CONVERSION DYNODE AND ELECTRON MULTIPLIER POWER BATTERY BACKUP VENT DELAY PCB ION GAUGE RF VOLTAGE AMPLIFIER ANALYZER PCB CONVECTRON GAUGE VENT VALVE ANALYZER AUXILIARY PCB WAVEFORM AMPLIFIER PCB Figure 7-5. PCBs and assemblies that are accessable from the top of the MS detector 7RUHSODFHWKH9HQW'HOD\3&%YHQWYDOYHRULRQJDXJHSURFHHG DVIROORZV 7. Shut down and vent the LCQ system as described in the topic Shutting Down the System Completely in the System Shutdown, Startup, and Reset chapter. Finnigan: LCQ MS Detector Hardware Manual 7-23

257 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing PCBs and Assemblies in the MS Detector :$51,1*0DNHVXUHWKDWWKH/&4SRZHUFRUGLVXQSOXJJHG EHIRUH\RXSURFHHG :$5181*'DV/&4PX YRP1HW]JHWUHQQWZHUGHQEHYRU LUJHQGZHOFKH(LQJULIIHYRUJHQRPQHQZHUGHQ $9(57,66(0(179HULILH]TXHOHFRUGRQGªDOLPHQWDWLRQGX /&4VRLWGpEUDQFKpGHODSULVHGHFRXUDQWDYDQWGHSURFpGHU 1. Remove the top cover of the MS detector as described in the topic Removing the Top Cover of the MS Detector in the MS Detector Maintenance chapter. To replace the Vent Delay PCB, go to step 3. To replace the vent valve, go to step 4. To replace the ion gauge, go to step To replace the Vent Delay PCB, proceed as follows: a. Disconnect at J1 the cable to the vent valve. b. Disconnect at J2 the cable to the Power Module. c. With a Phillips screwdriver, loosen the four screws that secure the Vent Delay PCB to the MS detector chassis. Remove the Vent Delay PCB. G 8QSDFNWKHQHZ9HQW'HOD\3&%31 5HWDLQWKHSDFNLQJPDWHULDOVVRWKDW\RXFDQSDFNDQG VKLSWKHGHIHFWLYH3&%WRWKH)LQQLJDQ5HSDLU&HQWHULQ 6DQ-RVH%HVXUHWRQRWHWKHDSSDUHQWSUREOHPRU V\PSWRPVRQWKHHQFORVHGIRUPV 7-24 Finnigan: LCQ MS Detector Hardware Manual

258 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing PCBs and Assemblies in the MS Detector H I 7HVWWKHEDWWHU\5HSODFHWKHEDWWHU\31 LIQHFHVVDU\ 3RVLWLRQWKHQHZ9HQW'HOD\3&%LQWKHVSDFHWKDWZDV RFFXSLHGE\WKHROG3&% g. With a Phillips screwdriver, tighten the four screws that secure the Vent Delay PCB. h. Reconnect at J1 the cable that goes to the vent valve. i. Reconnect at J2 the cable that comes from the Power Module. *RWRVWHS 3. To replace the vent valve, proceed as follows: a. Disconnect the cable that comes from the Vent Delay PCB. b. With a 7/16-in. open-end wrench, loosen the fitting to the vent valve solenoid. Remove the vent valve. c. Replace the old vent valve with a new one (P/N ) d. With a 7/16-in. open-end wrench, tighten the fitting to the vent valve solenoid. e. Reconnect the cable that comes from the Vent Delay PCB. *RWRVWHS 4. To replace the ion gauge, proceed as follows: a. Disconnect the cable from the top of the ion gauge by pulling it free from the ion gauge. b. Unscrew the ion gauge by hand from the vacuum manifold. c. Replace the old ion gauge with a new one (P/N ). Screw it into the vacuum manifold. d. Reattach the cable to the top of the ion gauge. Go to step 6 Finnigan: LCQ MS Detector Hardware Manual 7-25

259 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing PCBs and Assemblies in the MS Detector 5. Reinstall the top cover of the MS detector as described in the topic Reinstalling the Top Cover of the MS Detector in the MS Detector Maintenance chapter. 6. Restart the system as described in the topic Starting Up the System After a Complete Shutdown in the System Shutdown, Startup, and Reset chapter Replacing the Electron Multiplier and Conversion Dynode Power Supplies, Analyzer PCB, Analyzer Auxiliary PCB, Waveform Amplifier PCB, RF Voltage Amplifier PCB, and Battery Backup 7KHHOHFWURQPXOWLSOLHUSRZHUVXSSO\FRQYHUVLRQG\QRGHSRZHU VXSSO\$QDO\]HU3&%$QDO\]HU$X[LOLDU\3&%:DYHIRUP $PSOLILHU3&%5)9ROWDJH$PSOLILHU3&%DQGEDWWHU\EDWWHU\ EDFNXSDUHDFFHVVDEOHIURPWKHWRSRIWKH06GHWHFWRU6HH )LJXUHRQSDJH 7RUHSODFHWKHHOHFWURQPXOWLSOLHUSRZHUVXSSO\FRQYHUVLRQ G\QRGHSRZHUVXSSO\$QDO\]HU3&%$QDO\]HU$X[LOLDU\3&% :DYHIRUP$PSOLILHU3&%5)9ROWDJH$PSOLILHU3&%RUEDWWHU\ EDWWHU\EDFNXSSURFHHGDVIROORZV 3ODFHWKHHOHFWURQLFVVHUYLFHVZLWFKLQWKH6HUYLFH2))2 SRVLWLRQRUVKXWGRZQDQGYHQWWKH/&4V\VWHPDVGHVFULEHG LQWKHWRSLF6KXWWLQJ'RZQWKH6\VWHP&RPSOHWHO\LQWKH 6\VWHP6KXWGRZQ6WDUWXSDQG5HVHWFKDSWHU :$51,1*0DNHVXUHWKDWWKH/&4HOHFWURQLFVHUYLFHVZLWFKLV LQWKH6HUYLFH2))2SRVLWLRQRUVKXWGRZQWKHV\VWHPDQG GLVFRQQHFWWKHSRZHUFRUGEHIRUH\RXSURFHHG :$5181*.RQWUROOLHUHQ6LHGD GHU+DXSWVFKDOWHUI U/&4 (OHNWURQLNWHLOHDXI:DUWXQJ6HUYLFH2))2JHVFKDOWHWLVWRGHU VFKDOWHQ6LHGDV6\VWHPDEXQG]LHKHQ6LHGHQ1HW]VWHFNHUDE EHYRU6LHIRUWIDKUHQ 7-26 Finnigan: LCQ MS Detector Hardware Manual

260 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing PCBs and Assemblies in the MS Detector $9(57,66(0(176DVVXUHUTXHOHGLVMRQFWHXUGHEUDQFKHPHQW /&4HVWHQSRVLWLRQ6HUYLFH2))2RXPHWWUHOHV\VWqPHKRUV WHQVLRQHWGpFRQQHFWHUOHFRUGRQGDOLPHQWDWLRQDYDQWGH FRQWLQXHU 2. Remove the top cover of the MS detector as described in the topic Removing the Top Cover of the MS Detector in the MS Detector Maintenance chapter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innigan: LCQ MS Detector Hardware Manual 7-27

261 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing PCBs and Assemblies in the MS Detector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b. Disconnect (at P4) the lenses cable that comes from the System Control PCB. c. Disconnect (at P2 and P3) the two endcap electrode cables that come from the Analyzer Auxiliary PCB Finnigan: LCQ MS Detector Hardware Manual

262 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing PCBs and Assemblies in the MS Detector d. Disconnect (at P1) the electrometer cable. (If necessary, use a small screw driver to loosen the screws that secure the cable.) e. Disconnect the electron multiplier high voltage cable that comes from the electron multiplier power supply. f. Use a 7/16-in. open-end wrench to disconnect the helium damping gas line from the fitting. g. With a Phillips screwdriver, remove the metal cover from the Analyzer PCB. h. With a Phillips screwdriver, remove the screws that hold the Analyzer PCB to the top cover plate of the vacuum manifold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p. Reconnect (at P4) the lenses cable that comes from the System Control PCB. Finnigan: LCQ MS Detector Hardware Manual 7-29

263 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing PCBs and Assemblies in the MS Detector q. Reconnect (at P2 and P3) the two endcap cables that come from the Analyzer Auxiliary PCB. r. Reconnect (at P1) the electrometer cable. V 5HFRQQHFWWKHHOHFWURQPXOWLSOLHUKLJKYROWDJHFDEOHWKDW FRPHVIURPWKHHOHFWURQPXOWLSOLHUSRZHUVXSSO\ Go to step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innigan: LCQ MS Detector Hardware Manual

264 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing PCBs and Assemblies in the MS Detector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innigan: LCQ MS Detector Hardware Manual 7-31

265 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing PCBs and Assemblies in the MS Detector D E F G H I J :LWKD3KLOOLSVVFUHZGULYHUUHPRYHWKHPHWDOFRYHUIURP WKH5)9ROWDJH$PSOLILHU3&% :LWKD3KLOOLSVVFUHZGULYHUUHPRYHWKHVFUHZVWKDW VHFXUHWKH5)9ROWDJH$PSOLILHU3&%WRWKHWRSRIWKH WRZHU5HPRYHWKH5)9ROWDJH$PSOLILHU3&% 8QSDFNWKHQHZ5)9ROWDJH$PSOLILHU3&%31 5HWDLQWKHSDFNLQJPDWHULDOVVRWKDW\RXFDQ SDFNDQGVKLSWKHGHIHFWLYH3&%WRWKH)LQQLJDQ5HSDLU &HQWHULQ6DQ-RVH%HVXUHWRQRWHWKHDSSDUHQW SUREOHPRUV\PSWRPVRQWKHHQFORVHGIRUPV,QVWDOOWKHQHZ3&%LQWKHSODFHRFFXSLHGE\WKHROG3&% :LWKD3KLOOLSVVFUHZGULYHUUHLQVWDOOWKHVFUHZVWKDW VHFXUHWKH5)9ROWDJH$PSOLILHUWRWKHWRSRIWKHWRZHU 5HLQVWDOOWKHPHWDOFRYHUWRWKHWRSRIWKH5)9ROWDJH $PSOLILHU3&% 5HFRQQHFWDOOFDEOHVWRWKH5)9ROWDJH$PSOLILHU3&%WKDW \RXGLVFRQQHFWHGLQVWHSD *RWRVWHS 7RUHSODFHWKHEDWWHU\RQWKH9HQW'HOD\3&%SURFHHGDV IROORZV6HH)LJXUHRQSDJHIRUWKHORFDWLRQRIWKH EDWWHU\ D E 5HPRYHWKHEDWWHU\IURPWKH9HQW'HOD\3&% 5HLQVWDOODQHZEDWWHU\31LQWKHSODFH RFFXSLHGE\WKHROGEDWWHU\ *RWRVWHS 7. Reinstall the top cover of the MS detector as described in the topic Reinstalling the Top Cover of the MS Detector in the MS Detector Maintenance chapter. 8. Place the electronics service switch in the Normal (ON, ) position. 5XQWKH/&4GLDJQRVWLFVWRYHULI\WKDWWKHV\VWHPLV RSHUDWLRQDO,I\RXUHSODFHGWKH$QDO\]HU3&%$QDO\]HU$X[LOLDU\3&%RU 5)9ROWDJH$PSOLILHU3&%WXQHWKHULQJHOHFWURGHDQG 7-32 Finnigan: LCQ MS Detector Hardware Manual

266 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing PCBs and Assemblies in the MS Detector RFWDSROH5)YROWDJHVDVGHVFULEHGLQWKHWRSLF7XQLQJWKH 5LQJ(OHFWURGHDQG2FWDSROH5)9ROWDJHVLQWKH06 'HWHFWRU0DLQWHQDQFHFKDSWHU Replacing the System Control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qPHKRUV WHQVLRQHWGpFRQQHFWHUOHFRUGRQGDOLPHQWDWLRQDYDQWGH FRQWLQXHU 2. Remove the top cover of the MS detector as described in the topic Removing the Top Cover of the MS Detector in the MS Detector Maintenance chapter. 3. Remove the right side cover of the MS detector as follows: Finnigan: LCQ MS Detector Hardware Manual 7-33

267 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing PCBs and Assemblies in the MS Detector a. Loosen the fastener that secures the right side cover to the chassis of the MS detector. b. Slide the side cover back about 1.25 cm (0.5 in.), and then lift it out and away from the MS detector. 4. Remove the protective cover on the System Control PCB as follows: a. Insert a finger into the finger hole on the left side of the cover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innigan: LCQ MS Detector Hardware Manual

268 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing PCBs and Assemblies in the MS Detector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innigan: LCQ MS Detector Hardware Manual 7-35

269 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing PCBs and Assemblies in the MS Detector,RQJDXJH&RQYHFWURQJDXJH3 6ZLWFKLQJSRZHUVXSSOLHV- (OHFWURQPXOWLSOLHUFRQYHUVLRQG\QRGHSRZHUVXSSOLHV 3 $3&,KHDWHU- +LJKVSHHGVHULDOOLQH3 N9SRZHUVXSSO\- 13. Reinstall the protective cover over the System Control PCB as follows: a. Insert the three tabs on the left side of the cover into the corresponding three slots in the MS detector chassis. b. Swing the right side of the cover toward the chassis until the tabs in the cover insert and lock into the slots in the chassis. 5HLQVWDOOWKHULJKWVLGHFRYHURIWKH06GHWHFWRUDVIROORZV D 3ODFHWKHFRYHUDJDLQVWWKHULJKWVLGHRIWKH06GHWHFWRU VXFKWKDWWKHVWXGVRQWKHFRYHULQVHUWLQWRWKHJXLGHVORWV LQWKH06GHWHFWRUFKDVVLV b. Slide the side cover forward about 1.25 cm (0.5 in.) until the studs on the cover lock in the guide slots. F 7LJKWHQE\KDQGWKHIDVWHQHUWKDWVHFXUHVWKHVLGHFRYHU WRWKHFKDVVLVRIWKH06GHWHFWRU 15. Reinstall the top cover of the MS detector as described in the topic Reinstalling the Top Cover of the MS Detector in the MS Detector Maintenance chapter. 16. Place the electronics service switch in the Normal (ON, ) position. 5XQWKH/&4GLDJQRVWLFVWRYHULI\WKDWWKHV\VWHPLV RSHUDWLRQDO 7-36 Finnigan: LCQ MS Detector Hardware Manual

270 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing PCBs and Assemblies in the MS Detector Replacing the RF Voltage Control PCB 7RUHSODFHWKH5)9ROWDJH&RQWURO3&%DQGLWVKRXVLQJSURFHHG DVIROORZV6HH)LJXUHIRUWKHORFDWLRQRIWKH5)9ROWDJH &RQWURO3&% 3ODFHWKHHOHFWURQLFVVHUYLFHVZLWFKLQWKH6HUYLFH2))2 SRVLWLRQRUVKXWGRZQDQGYHQWWKH/&4V\VWHPDVGHVFULEHG LQWKHWRSLF6KXWWLQJ'RZQWKH6\VWHP&RPSOHWHO\LQWKH 6\VWHP6KXWGRZQ6WDUWXSDQG5HVHWFKDSWHU RF VOLTAGE CONTROL PCB Figure 7-6. Left side of the MS detector, showing the RF Voltage Control PCB Finnigan: LCQ MS Detector Hardware Manual 7-37

271 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing PCBs and Assemblies in the MS Detector :$51,1*0DNHVXUHWKDWWKH/&4HOHFWURQLFVHUYLFHVZLWFKLV LQWKH6HUYLFH2))2SRVLWLRQRUVKXWGRZQRUVKXWGRZQWKH V\VWHPDQGGLVFRQQHFWWKHSRZHUFRUGEHIRUH\RXSURFHHG :$5181*.RQWUROOLHUHQ6LHGD GHU+DXSWVFKDOWHUI U/&4 (OHNWURQLNWHLOHDXI:DUWXQJ6HUYLFH2))2JHVFKDOWHWLVWRGHU VFKDOWHQ6LHGDV6\VWHPDEXQG]LHKHQ6LHGHQ1HW]VWHFNHUDE EHYRU6LHIRUWIDKUHQ $9(57,66(0(176DVVXUHUTXHOHGLVMRQFWHXUGHEUDQFKHPHQW /&4HVWHQSRVLWLRQ6HUYLFH2))2RXPHWWUHOHV\VWqPHKRUV WHQVLRQHWGpFRQQHFWHUOHFRUGRQGDOLPHQWDWLRQDYDQWGH FRQWLQXHU 2. Remove the top cover of the MS detector as described in the topic Removing the Top Cover of the MS Detector in the MS Detector Maintenance chapter. 3. Remove the left side cover of the MS detector as follows: a. Loosen the fastener that secures the left side cover to the chassis of the MS detector. b. Slide the side cover back about 1.25 cm (0.5 in.), and then lift it out and away from the MS detector. &DXWLRQ7RSUHYHQWGDPDJHWRWKHHOHFWURQLFVGXHWRHOHFWURVWDWLF GLVFKDUJHDWWDFKDQHOHFWURVWDWLFGLVFKDUJH(6'VWUDSWR\RXU ZULVWEHIRUHFRQWLQXLQJ :LWKD3KLOOLSVVFUHZGULYHUUHPRYHWKHQLQHVFUHZVWKDW VHFXUHWKHIURQWFRYHURIWKH5)9ROWDJH&RQWURO3&%5HPRYH WKHIURQWFRYHUWRH[SRVHWKH5)9ROWDJH&RQWURO3&% 7-38 Finnigan: LCQ MS Detector Hardware Manual

272 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing PCBs and Assemblies in the MS Detector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b. Slide the side cover forward about 1.25 cm (0.5 in.) until the studs on the cover lock in the guide slots. F 7LJKWHQE\KDQGWKHIDVWHQHUWKDWVHFXUHVWKHVLGHFRYHU WRWKHFKDVVLVRIWKH06GHWHFWRU Finnigan: LCQ MS Detector Hardware Manual 7-39

273 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing PCBs and Assemblies in the MS Detector 13. Reinstall the top cover of the MS detector as described in the topic Reinstalling the Top Cover of the MS Detector in the MS Detector Maintenance chapter. 14. Place the electronics service switch in the Normal (ON, ) position. 5XQWKH/&4GLDJQRVWLFVWRYHULI\WKDWWKHV\VWHPLV RSHUDWLRQDO Replacing the Low Pass Filter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qPHKRUV WHQVLRQHWGpFRQQHFWHUOHFRUGRQGDOLPHQWDWLRQDYDQWGH FRQWLQXHU 7-40 Finnigan: LCQ MS Detector Hardware Manual

274 DIAGNOSTICS AND PCB AND ASSEMBLY REPLACEMENT Replacing PCBs and Assemblies in the MS Detector 4. Remove the top cover of the MS detector as described in the topic Removing the Top Cover of the MS Detector in the MS Detector Maintenance chapter. 5. Remove the rear cover of the MS detector as follows: a. With a Phillips screwdriver, loosen the 10 screws that secure the rear cover to the chassis of the MS detector. b. Slide the rear cover up about 1.25 cm (0.5 in.), and then lift it out and away from the MS detector. 'LVFRQQHFWWKHWZRFRD[LDOFDEOHVIURPWKH%1&FRQQHFWRUV WKDWDUHORFDWHGRQWKHUHDURIWKH/RZ3DVV)LOWHU3&% &DXWLRQ7RSUHYHQWGDPDJHWRWKHHOHFWURQLFVGXHWRHOHFWURVWDWLF GLVFKDUJHDWWDFKDQHOHFWURVWDWLFGLVFKDUJH(6'VWUDSWR\RXU ZULVWEHIRUHFRQWLQXLQJ VENT DELAY PCB BATTERY BACKUP FAN, LOW PASS FILTER, VENT CONTROL ASSEMBLY LOW PASS FILTER Figure 7-7. Rear view of the MS detector, showing the Vent Delay PCB, battery backup, and Low Pass Filter PCB Finnigan: LCQ MS Detector Hardware Manual 7-41