Cell cycle machinery M G1
|
|
|
- Archibald Jackson
- 6 years ago
- Views:
Transcription
1 Cell cycle machinery M G1 G2 S
2 D A cdk1 cdk1 B M G2 G1 S Cdk4/6 E E2F P P P P Rb P P A
3 cdk1 B M G1 D1 Cdk4/6 E1 D2 Cdk4/6 Cdk4/6 D3 E2 E2 A2 A1 G2 S E2 A2 A1
4 cyc A cyc E cyc D G1 S G2 M
5 E-CYCLINS cyclin E1 and E2 associate with CDK2 (CDK3 or CDK1) rapidly induced prior to the S phase entry overexpression shortens G1 phase antibodies block S phase entry cyclin E gene on 19q12 amplified in several cancers cyclin E overexpressed in ~25% of breast cancers
6 Functions for E-CYCLINS Retinoblastoma protein (prb) inactivation Cell cycle progression (p27 Kip1, CDC25) DNA replication initiation (CDC45 loading) Centrosome duplication (nucleophosmin) Histone biosynthesis (p220 NPAT) E-cyclins are absolutely essential for cell cycle progresssion
7 EXPRESSION OF CYCLINS E1 AND E2 cyclin E1 cyclin E2 BrdU E9.5 n n b m E11.5 n n
8 cdk1 B M G1 D1 Cdk4/6 E1 D2 Cdk4/6 Cdk4/6 D3 E2 E2 A2 A1 G2 S E2 A2 A1
9 Cyclin E1 knockout cyclin E1 genomic structure Xho I Asp I Xba I Xba I Xba I Spe I Sca I Not I Spe I Xho I Sca I Hpa I Not I Neo (ScaI) Spe I cyclin E1 targeting strategy Spe I Sca I Hpa I 1kb WT E1 +/- E1 -/- 7.6kb 2.8kb 2.6kb
10 Cyclin E1 -/- mice viable develop normally
11 cdk1 B M G1 D1 Cdk4/6 E1 D2 Cdk4/6 Cdk4/6 D3 E2 E2 A2 A1 G2 S E2 A2 A1
12 Cyclin E2 knockout cyclin E2 genomic structure Not I EcoR I Sph I Sph I Not I EcoR I Spe I Spe I Spe I Spe I Aat II Puro Sph I Sph I Not I Not I cyclin E2 targeting strategy Aat II 1kb WT E2 -/- E2 +/- 9.0kb with Bruno Amati
13 Cyclin E2 -/- mice viable some males sterile testicular hypoplasia reduced sperm counts
14 cdk1 B M G1 D1 Cdk4/6 E1 D2 Cdk4/6 Cdk4/6 D3 E2 E2 A2 A1 G2 S E2 A2 A1
15 Cyclin E1 -/- E2 -/- Embryonic lethal
16 Cyclin E1 -/- E2 -/- embryos Observed Expected E /111 7/111 E (13)/168 16/168 E11.5 3(6)/166 18/166 E12.5 (4)/82 10/82
17 CYCLIN E1 -/- E2 -/- EMBRYOS Control E1 -/- E2 -/- day E 10.75
18 Phenotype of trophoblast giant cells WT *
19 Phenotype of trophoblast giant cells WT * * E1 -/- E2 -/-
20 ENDOREPLICATION G S
21 % of nuclei Phenotype of trophoblast giant cells 80 WT E1 -/- E2 -/- * * E1 -/- E2 -/- WT < >900 Ploidy (n)
22 Cyclin E1 -/- E2 -/- mice E9.0 - E10.75 Normal embryo development Abnormal placental development Paucity of trophoblast giant cells Endoreplication problem
23 Mate E1 +/- E2 +/- mice E1 -/- E2 -/- blastocysts E1 -/- E2 -/- ES cells
24 2n 2n 4n 4n
25 2n 2n 4n E1 -/- E2 -/- ES Cells 4n
26 Rescued cyclin E-null neonates WT E1 -/- E2 -/-
27 Cardiac abnormalities in cyclin E-null neonates WT WT E1 -/- E2 -/- E1 -/- E2 -/-
28 Cyclin E is largely dispensable for development
29 cdk1 B M G1 D1 Cdk4/6 E1 D2 Cdk4/6 Cdk4/6 D3 E2 E2 A2 A1 G2 S E2 A2 A1
30 cdk1 B M G1 D1 Cdk4/6 E1 D2 Cdk4/6 Cdk4/6 D3 E2 E2 A1 cdk1 A2 cdk1 G2 S E2 A1 A2
31 Role of cyclin A in the cell cycle Regulate DNA synthesis induce the initiation of DNA replication prevent re-replication Plays a role in centrosome duplication Entry into mitosis
32 A1 A2
33 Expression pattern of cyclins A1 and A2 A1 A2 C. Sweeney et al., 1996
34 A1 A1 A2 A2 A1 Testis
35 cyclin A2 is an essential component of all embryonic and somatic cell cycles in mammals there are essentially two types of cyclins: those that are essential components of the cell cycle engine [cyclins A and B], and those involved in cell type specific regulation of the cycle [G1 cyclins]
36 Consequence of cyclin A2 deletion in mice Ccna2 -/- mouse fail to develop past 5.5 days pc (Murphy at al., 1997)
37 Conditional knockout of cyclin A2 A2
38 Cyclin A2 conditional construct E1 E2 E3 E4 E5 E6 E7 E8 lox P lox P pgk Neo ATG FRT FRT In vitro flp mediated recombination STOP In vitro or in vivo CRE mediated recombination E1 E8
39 Cyclin A2 expression level after Cre mediated recombination A2 A2 -actin β-actin
40 Absorbance at 595 nm In vitro proliferation of cyclin A deficient fibroblasts 0,8 0,7 A2 F/F A2 / 0,7 0,6 A1 -/- A2 F/F A1 -/- A2 / 0,6 0,5 0,5 0,4 0,4 0,3 0,3 0,2 0,2 0,1 0, Days Days
41 Re-entry of cell cycle by cyclin A deficient fibroblasts [3H]-thymidine incorporation x 10 3 [cpm] 3 2,5 2 1,5 1 0,5 A2 F/F A2 / 5 4,5 4 3,5 3 2,5 2 1,5 1 0,5 A1 -/- A2 F/F A1 -/- A2 / Hours Hours
42 Cell cycle profile of MEFs lacking cyclin A2 A2 F/F G1: 48.2% S: 19.4 % G2/M: 25 % A2 / G1: 32.2 % S: 25.9 % G2/M: 29.4 %
43 A1 -/- x A2 F/F D. Wolgemuth
44 Cyclin A2 expression level after Cre mediated recombination A2 actin
45 Absorbance at 595 nm In vitro proliferation of cyclin A deficient fibroblasts 0,8 0,7 A2 F/F A2 / 0,7 0,6 A1 -/- A2 F/F A1 -/- A2 / 0,6 0,5 0,5 0,4 0,4 0,3 0,3 0,2 0,2 0,1 0, Days Days
46 Re-entry of cell cycle by cyclin A deficient fibroblasts [3H]-thymidine incorporation x 10 3 [cpm] 3 2,5 2 1,5 1 0,5 A2 F/F A2 / 5 4,5 4 3,5 3 2,5 2 1,5 1 0,5 A1 -/- A2 F/F A1 -/- A2 / Hours Hours
47 Cell cycle profile of MEF lacking cyclins A1 & A2 A1 -/- A2 F/F G1: % S: 18.2 % G2/M: 21.6 % A1 -/- A2 G1: 39 % S: 22.6 % G2/M: 30 %
48 % of cells P< P< Control Ряд P= P= P= Ряд2 A2 / A1 -/- A2 / Ряд P= G1 S G2/M
49 G1 Control S G2/M G1 S G2/M A1 -/- A2
50 Absorbance at 595 nm In vitro proliferation of cyclin A deficient fibroblasts 0,8 0,7 A2 F/F A2 / 0,7 0,6 A1 -/- A2 F/F A1 -/- A2 / 0,6 0,5 0,5 0,4 0,4 0,3 0,3 0,2 0,2 0,1 0, Days Days
51 cdk1 B M G1 D1 Cdk4/6 E1 D2 Cdk4/6 Cdk4/6 D3 E2 E2 A1 cdk1 A2 cdk1 G2 S E2 A1 A2
52 The levels of cell cycle regulators in cyclin A-deficient fibroblasts D1 D2 Cdk1 Cdk2 Cdk4 Cdk6 p27 E1 A2 β-actin actin E1 A2 β-actin
53 cyc A cyc E cyc D G1 S G2 M
54 Relative level The levels of cell cycle regulators in cyclin A-deficient fibroblasts D1 D2 D3 E1 A2 Control A1 -/- A hours 1,2 1 0,8 0,6 0,4 0,2 Cyclin E level in control Cyclin E level in A1 -/- A2 Cyclin A2 level in control Ряд1 Ряд2 Ряд Hours
55 Cyclin A-Cdk and E-cdk complexes E E cdk1 A A cdk1
56 Cyclin A-Cdk and E-cdk complexes E E cdk1 Cdk1-kinase Cdk2-kinase A A cdk1
57 prb T821 NPM T199 B-Myb S581 B-Myb T490 Cdc6 S54 Smad3 T8 Smad3 S212 RRM2 p107 PCNA Mcm2 E2F1 B-Myb β-actin
58 cdk1 B M G1 D1 Cdk4/6 E1 D2 Cdk4/6 Cdk4/6 D3 E2 E2 A1 cdk1 A2 cdk1 G2 S E2 A1 A2
59 -/F/F A1 A2 -/-/E1 E2
60 E1 E2
61 E1 E2
62 Cyclin E1 -/- E2 -/- day E Yan Geng
63 CYCLIN E1 -/- E2 -/- MICE Control E1 -/- E2 -/-
64 Mate E1 +/- E2 +/- mice E1 -/- E2 -/- blastocysts E1 -/- E2 -/- ES cells
65 2n 2n 4n 4n
66 2n 2n 4n E1 -/- E2 -/- ES Cells 4n
67 A1 -/- A2 F/F E1 -/- E2 +/- x A1 +/- A2 F/F E1 +/- E2 +/- A1 -/- A2 F/F E1 -/- E2 -/- A1 -/- A2 F/F E1 -/- E2 -/- ES cells 4n blastocyst A1 -/- A2 F/F E1 -/- E2 -/- MEFs + Cre A1 -/- A2 E1 -/- E2 -/- MEFs A2
68 BrdU + cells [%] Analysis of fibroblasts lacking all A- and E-type cyclins Cell number x 10 5 [3H]-thymidine incorporation x 10 3 [cpm] Control A1 + Cre + Control + Cre -/- A2 / E1 -/- E2 -/- A1 -/- A2 f/f E1 -/- E2 -/- A1 -/- A2 f/f E1 -/- E2 -/- A1 -/- A2 f/f E1 -/- E2 -/- 44% % 14% % 40% 16%
69 Analysis of fibroblasts lacking all A- and E-type cyclins 7 A1 -/- A2 f/f E1 -/- E2 -/- A1 -/- A2 / E1 -/- E2 -/- 6 Cell number x Days
70 Cdk1-kinase Cdk1-kinase Cdk2-kinase Cdk2-kinase
71 cdk1 B M G1 D1 Cdk4/6 E1 D2 Cdk4/6 Cdk4/6 D3 E2 E2 A1 cdk1 A2 cdk1 G2 S E2 A1 A2
72 Debra Wolgemuth Columbia Hirokazu Shigematsu Tadafumi Iino Shin-ichi Mizuno Koichi Akashi
73 Consequence of cyclin A deletion in vivo Bone marrow specific deletion in Mx A1 -/- A2 F/F mice
74 Cyclin A2 expression level in A2 F/F Mx1 mice Before and after poly(i) poly (C) treatment A2 A2 β-actin A2 F/F WT
75 % Survival Kaplan-Meier survival graph 100 Mx control n=18 25 Mx P<0,0001 A2 Mx A1 -/- A Days Time after 6x pipc injection
76 Cell number x 10 6 /ul Cell number x 10 3 /ul Cell number x 10 5 /ul Сотни Peripheral blood analysis 12 RBC WBC PLT Mx control Mx A2 Mx A1 -/- A n=
77 Analysis of bone marrow cells Cell number x 10 6 / 2 femurs Mx control Mx A2 Mx A1 -/- A2 n= H&E
78 Cell number x 10 6 / femur Analysis of BM subpopulations Mx control 25 Mx A Mx A1 -/- A2 n= Gr-1 Mac-1 Ter119 B220 CD3 Granulocytes Monocytes Erythroid lineage B cells T cells
79 At which stage of hematopoiesis cyclin A is rate-limiting?
80 Cell number x 10 3 / 2 femurs Cell number x 10 3 / 2 femurs Analysis of hematopoetic stem cells and progenitors Mx control Mx A2 80 Mx A1 -/- A HSC CMP GMP MEP Tadafumi Iino Shin-ichi Mizuno Koichi Akashi
81 Gr-1 Gr-1 Gr-1 Analysis of progenitors Mx control Mx A2 Mx A1 -/- A Mac Mac Mac-1 Tadafumi Iino Shin-ichi Mizuno Koichi Akashi
82 Number of colonies Analysis of Hematopoetic Stem Cells Before Cre A1 +/- A2 f/+ A1 -/- A2 f/f A A1 +/- A1 +/- A2 /+ A1 -/- A1 -/- A2 F/+ A2 F/ A2 A2 + After Cre A1 +/- A2 /+ A1 -/- A2 f/ A2 f A1 +/- A2 F/+ +Cre A1 -/- A2 F/F Tadafumi Iino Shin-ichi Mizuno Koichi Akashi
83 Embryonic stem cells A1 -/- A2 F/F
84 Embryonal stem cells A1 -/- A2 F/F +Cre
85 Number of colonies Analysis of embryonal stem cells A1 +/- A2 f/+ A1 +/- A2 /+ A1 -/- A1 -/- A2 f/ 0 0 A1 -/- A2 / A1 -/- A1 -/- A2 f/f A2 f/ A2 / +Cre +Cre A1 +/- A2 f/+ A1 -/- A2 f/f A1 -/- A2 f/
86 Proliferation of MEFs does NOT depend on cyclin A but Proliferation (survival) of HSC and ES cells critically depends on cyclin A What is the molecular basis of this difference?
87 Mammalian cell cycle in stem cells Rb E2F A cdk1 cdk1 B M G2 G1 S D Cdk4/6 E E2F P P P Rb E2F P P P P Rb P P A
88 Debra Wolgemuth Columbia Hirokazu Shigematsu Tadafumi Iino Shin-ichi Mizuno Koichi Akashi
Warning: software often displays unrooted trees like this:
Warning: software often displays unrooted trees like this: /------------------------------ Chara /-------------------------- Chlorella /---------16 \---------------------------- Volvox +-------------------17
Babaev et al. SUPPLEMENTAL DATA. Oil-Red-O MOMA-2. Lesion Area (μm 2 x10 3 ) Lesion Area(% of total) WT Akt1 -/- WT Akt1 -/-
SUPPLEMENTL T Oil-Red-O MOM-2 WT Ldlr -/- kt1 -/- Ldlr -/- WT Ldlr -/- kt1 -/- Ldlr -/- Lesion rea(% of total) 2. 1..5. Lesion rea (μm 2 x1 3 ) 2 15 1 5 WT kt1 -/- WT kt1 -/- Figure S1. Hematopoietic kt1
In this talk I will be talking about improving the accuracy of S phase estimation from cytometric data containing DNA content. A new method of interpo
In this talk I will be talking about improving the accuracy of S phase estimation from cytometric data containing DNA content. A new method of interpolation, parabolic splines (PS), for Probability State
T H E J O U R N A L O F C E L L B I O L O G Y
T H E J O U R N A L O F C E L L B I O L O G Y Supplemental material Gusnowski and Srayko, http://www.jcb.org/cgi/content/full/jcb.201103128/dc1 Figure S1. EB1-GFP velocities from the centrosomes. (A) Mean
CELL-LINES: TRANSCRIPTION THERAPY BY RA
PML/ PML/ RAR RAR CELL-LINES: TRANSCRIPTION THERAPY BY RA HDAC Co-repressors HDAC RA 1-6 M Coactivators HAT PML-INDUCED DIMERISATION: ENHANCED COREPRESSOR BINDING RARA AND REPRESSION BY HISTONE DEACETYLATION
Reviewers' comments: Reviewer #1 (Remarks to the Author):
Reviewers' comments: Reviewer #1 (Remarks to the Author): This paper evaluates the requirements for recruitment of BLM to DSBs on a quasi-global basis and as a function of the cell cycle using AsiSI to
Immunomagnetic Cell Separation
Immunomagnetic Cell Separation 17 2 Immunomagnetic Cell Separation Catherine Clarke and Susan Davies 1. Introduction In metastasis research, it may sometimes be necessary to separate populations of tumor
Strategic Futures Planning
Strategic Futures Suggestions for Success Author: Andrew Jackson, Deputy Director Foresight Foresight and the OST Horizon Scanning Centre are run by the Office of Science and Technology in the Department
Strong reversal of the lung fibrosis disease signature by autotaxin inhibitor GLPG1690 in a mouse model for IPF
Strong reversal of the lung fibrosis disease signature by autotaxin inhibitor GLPG1690 in a mouse model for IPF Maté Ongenaert, PhD Senior Scientist Bioinformatics Sonia Dupont, Roland Blanqué, Reginald
HoloMonitor M4. For powerful discoveries in your incubator
HoloMonitor M4 For powerful discoveries in your incubator HoloMonitor offers unique imaging capabilities that greatly enhance our understanding of cell behavior, previously unachievable by other technologies
Fig. S1. HEC1 and SPT are co-expressed during gynoecium development.
Fig. S1. HEC1 and SPT are co-expressed during gynoecium development. (A) Global expression profile of HEC1 and SPT during Arabidopsis development. Mean normalized data is from the AtGenExpress atlas (Schmid
AC DermaPeptide Toning PF Anti-Wrinkle + Toning + Elasticity
AC DermaPeptide Toning PF Anti-Wrinkle + Toning + Elasticity AC DermaPeptide Toning PF Technical Information Code: 20455PF INCI Name: Water & Yeast Extract Status: Conforms Suggested Use Levels: 2.0 5.0%
HoloMonitor. Phase. For competent and powerful discoveries. Holographic time-lapse imaging cytometry
HoloMonitor M4 Holographic time-lapse imaging cytometry For competent and powerful discoveries Monitor and quantify living cells in their natural environment with unrivaled temporal resolution Phase Holographic
National and European-level strategies for implementing open access research infrastructures. Euro-BioImaging Stakeholder meeting Wien, Jan 21, 2013
National and European-level strategies for implementing open access research infrastructures Euro-BioImaging Stakeholder meeting Wien, Jan 21, 2013 Eero Vuorio Own perspectives 1. Director of Biocenter
Human Papillomavirus Type 31 E5 Protein Supports Cell Cycle Progression and Activates Late Viral Functions upon Epithelial Differentiation
JOURNAL OF VIROLOGY, Mar. 2003, p. 2819 2831 Vol. 77, No. 5 0022-538X/03/$08.00 0 DOI: 10.1128/JVI.77.5.2819 2831.2003 Copyright 2003, American Society for Microbiology. All Rights Reserved. Human Papillomavirus
PRODUCT CATALOG CYTOKINES GROWTH FACTORS INTERLEUKINS CHEMOKINES PROTEIN BUNDLES 2017
PRODUCT CATALOG CYTOKINES GROWTH FACTORS INTERLEUKINS 2017 CHEMOKINES PROTEIN BUNDLES SHENANDOAH BIOTECHNOLOGY began in April of 2006. Our home office and lab are conveniently located in Warwick, Pennsylvania,
Genomic Analysis of hesc Pedigrees Identifies De Novo Mutations and Enables Determination of the Timing and Origin of Mutational Events
lease cite this article in press as: en-yosef et al., enomic Analysis of hesc edigrees Identifies De Novo Mutations and Enables Determination of Cell Reports Resource enomic Analysis of hesc edigrees Identifies
Supporting Online Material for
www.sciencemag.org/cgi/content/full/312/5782/1944/dc1 Supporting Online Material for The Competitive Cost of Antibiotic Resistance in Mycobacterium tuberculosis Sebastien Gagneux,* Clara Davis Long, Peter
ESSENTIAL ELEMENT, LINKAGE LEVELS, AND MINI-MAP SCIENCE: HIGH SCHOOL BIOLOGY SCI.EE.HS-LS1-1
State Standard for General Education ESSENTIAL ELEMENT, LINKAGE LEVELS, AND MINI-MAP SCIENCE: HIGH SCHOOL BIOLOGY SCI.EE.HS-LS1-1 HS-LS1-1 Construct an explanation based on evidence for how the structure
SUPPLEMENTARY INFORMATION
Quantitative visualization of DNA G-quadruplex structures in human cells Giulia Biffi 1, David Tannahill 1, John McCafferty 2 & Shankar Balasubramanian 1,3 * 1 Cancer Research UK Cambridge Institute, Li
Title: JNK activation is essential for activation of MEK/ERK signaling in IL-1β-induced COX-2 expression in synovial fibroblasts
Title: JNK activation is essential for activation of MEK/ERK signaling in -induced COX-2 expression in synovial fibroblasts Author list: Taku Kitanaka 1,a, Rei Nakano 1,a, Nanako Kitanaka 1, Taro Kimura
Resilience and Intelligence
Resilience and Intelligence Katsumi Inoue National Institute of Informatics, Japan Systems Resilience Bridging the Gap Between Social and Mathematical Shonan Meeting, February 23, 2015 Contents 1. Intelligence
COMMUNITY UNIT SCHOOL DISTRICT 200 Science Curriculum Philosophy
COMMUNITY UNIT SCHOOL DISTRICT 200 Science Curriculum Philosophy Science instruction focuses on the development of inquiry, process and application skills across the grade levels. As the grade levels increase,
Evolution of U.S. Innovation Policy
Evolution of U.S. Innovation Policy Gregory Tassey Economic Analysis Office National Institute of Standards and Technology tassey@nist.gov October 2011 The Innovation Policy Challenge Characteristics of
CS4742 BIOINFORMATICS
CS4742 BIOINFORMATICS BIOLOGICAL NETWORK MOTIFS Programming today is a race between software engineers striving to build bigger and better idiot-proof programs, and the Universe trying to produce bigger
IN Cell Analyzer 2000
GE Healthcare IN Cell Analyzer 2000 Cell analysis just got easier Impressively enabling for cell analysis Developed with meticulous attention to the needs of the entire high-content imaging workflow,
35 th Annual International Asilomar Chromatin & Chromosomes Conference
SCIENTIFIC PROGRAM 35 th Annual International Asilomar Chromatin & Chromosomes Conference December 12-15, 2013 Asilomar Conference Grounds Pacific Grove, CA Organizers: Mike Goldman, San Francisco State
INFRAFRONTIER / IMPC Promoting the international exchange of mouse mutant resources. Martin Hrabé de Angelis, Helmholtz Zentrum München
INFRAFRONTIER / IMPC Promoting the international exchange of mouse mutant resources Martin Hrabé de Angelis, Helmholtz Zentrum München INFRAFRONTIER / IMPC Meeting, Munich, May 8th 2014 Resource and data
Tetraspanin-13 modulates voltage-gated Ca V 2.2 Ca 2+ channels
Tetraspanin-13 modulates voltage-gated Ca V 2.2 Ca 2+ channels Robert Mallmann 1,2, Thomas Wilmes 1, Lucia Lichvarova 3, Anja Bührer 1,2, Barbara Lohmüller 1,2, Jan Castonguay 1, Lubica Lacinova 3 and
Chapter 29. Quantitative X-ray Imaging of Rodent Bone by Faxitron. J.H. Duncan Bassett, Anne van der Spek, Apostolos Gogakos, and Graham R.
Chapter 29 Quantitative X-ray Imaging of Rodent Bone by Faxitron J.H. Duncan Bassett, Anne van der Spek, Apostolos Gogakos, and Graham R. Williams Abstract This chapter describes the use of digital micro-radiography
Exercise 8. Procedure. Observation
Exercise 8 Procedure Observe the slide under lower magnification of the microscope. In case of chart/models/photographs, note the feature of blastula in your practical record and draw labelled diagram.
You may view, copy, print, download, and adapt copies of this Social Science Bites transcript provided that all such use is in accordance with the
Sarah Franklin on the Sociology of Reproductive Technologies David Edmonds: In the sci-fi movie Gattaca potential children are carefully chosen using preimplantation genetic analysis. The movie taps into
Westwood Kansas Forum November 7, 2015 Part 3 Page 1 of 21
Westwood Kansas Forum November 7, 2015 Part 3 Page 1 of 21 Speakers: Suman Kambhampati, MD Jean Ridgeway, DNP, APN, NP-C, AOCN Jean Ridgeway: So, this is just to kind of give you a little bit of a glimpse
Alginate 3D Cell Culture Kit
Alginate 3D Cell Culture Kit 1/4 Catalogue No. AMS.CSR-ABC-KIT Transformed cells, such as tumor cells, have the characteristic feature of anchorage- independent growth, unlike normal cells. Some normal
capabilities today. Flexibility for tomorrow.
capabilities today. Flexibility for tomorrow. NEW CellStream benchtop flow cytometry system with Amnis detection technology inside The life science business of Merck KGaA, Darmstadt, Germany operates as
Exercise 4 Exploring Population Change without Selection
Exercise 4 Exploring Population Change without Selection This experiment began with nine Avidian ancestors of identical fitness; the mutation rate is zero percent. Since descendants can never differ in
Health Issues. Introduction. Ionizing vs. Non-Ionizing Radiation. Health Issues 18.1
Health Issues 18.1 Health Issues Introduction Let s face it - radio waves are mysterious things. Especially when referred to as electromagnetic radiation the concept makes many people nervous. In this
Elucidating tissue specific genes using the Benford distribution
Karthik et al. BMC Genomics (2016) 17:595 DOI 10.1186/s12864-016-2921-x RESEARCH ARTICLE Open Access Elucidating tissue specific genes using the Benford distribution Deepak Karthik, Gil Stelzer, Sivan
HM5 Quality Assurance & Histogram Interpretation. Dr. Melanie Hicks, BSc, DVM Senior Professional Services Veterinarian
HM5 Quality Assurance & Histogram Interpretation Dr. Melanie Hicks, BSc, DVM Senior Professional Services Veterinarian Topics 1) Quality Assurance Pre & Post- Analysis 2) HM5: how it works 3) Histograms
Osmotic stress induces the phosphorylation of WNK4 Ser575 via the p38mapk-mk pathway. Tokyo, Hongo, Bunkyo-ku, Tokyo , Japan
Osmotic stress induces the phosphorylation of WNK4 Ser575 via the p38mapk-mk pathway Junichi Maruyama 1, 2, Yumie Kobayashi 1, Tsuyoshi Umeda 1, Alain Vandewalle 3, Kohsuke Takeda 1, 4, Hidenori Ichijo
SCRIPPS DISCOVERS. A Newsletter for Philanthropists Published Quarterly by The Scripps Research Institute SPRING 2010 VOL 6 NO 2
SCRIPPS DISCOVERS Accelerating Discoveries, Saving Lives A Newsletter for Philanthropists Published Quarterly by The Scripps Research Institute SPRING 2010 VOL 6 NO 2 California-Florida I N S T I T U T
AccuNanoBead Ni-NTA Silica Magnetic Beads
AccuNanoBead Ni-NTA Silica Magnetic Beads The Best Solution for Biomolecule Purification 4. Applications of Magnetic Beads Cell separation Protein isolation and purification In vitro diagnostics, etc.
Illumina GenomeStudio Analysis
Illumina GenomeStudio Analysis Paris Veltsos University of St Andrews February 23, 2012 1 Introduction GenomeStudio is software by Illumina used to score SNPs based on the Illumina BeadExpress platform.
Evolution of the Y-Chromosome in Primates
Western Washington University Western CEDAR Western Libraries Undergraduate Research Award Western Libraries and the Learning Commons 2014 Evolution of the Y-Chromosome in Primates Audrey Marsh Western
Human Pedigree Genetics Answer Key
Human Pedigree Genetics Answer Key Free PDF ebook Download: Human Pedigree Genetics Answer Key Download or Read Online ebook human pedigree genetics answer key in PDF Format From The Best User Guide Database
"'" "'" 1. Chk1 phosphorylates BLM in vitro. 1.1 Generation ofpgex4t-1 BLM (1-1417) Step 1: Generation ofpgex-4t-1 BLM (1-1041)
SUMMARY Determining protein phosphorylation sites is often the first step in the elucidation of a regulatory mechanism and understanding the role of the protein. Knowledge about the phosphorylation sites
Multiplexing as Essential Tool for Modern Biology
Multiplexing as Essential Tool for Modern Biology Bio-Plex Seminar, Debrecen, 2012. Gyula Csanádi, PhD. The "Age of "-omics" Studying interrelationships at different level of complexity Genes - Unveiling
Confocal Microscope. Confocal Microscope C2
Confocal Microscope Confocal Microscope C2 Confocal Microscope An essential microscopy laboratory insturument The C2 confocal microscope system comprises a new generation of Nikon confocal instruments
CuB. An Innovative Diagnostic method Proposal for an INFN project
B Cu CuB An Innovative Diagnostic method Proposal for an INFN project 1 From Elemental imaging of a bee to.. A New Imaging System Fe K Zn Mn Ca Scientific Interest. Why? Do you need a new preclinical imaging?
Introduction. INSTRUCTION MANUAL CAT XL, 6500-XLCORE, 6500-FL Evos-XL, Evos-XL/Core, Evos-FL
1 INSTRUCTION MANUAL CAT. 6500-XL, 6500-XLCORE, 6500-FL Evos-XL, Evos-XL/Core, Evos-FL Introduction Experience faster results and easier cell imaging with an EVOS imaging system! An EVOS system is the
K.1 Structure and Function: The natural world includes living and non-living things.
Standards By Design: Kindergarten, First Grade, Second Grade, Third Grade, Fourth Grade, Fifth Grade, Sixth Grade, Seventh Grade, Eighth Grade and High School for Science Science Kindergarten Kindergarten
Discovering drugs by using mixed cell cultures
Discovering drugs by using mixed cell cultures Mark Anthony Bray, David Logan and Anne Carpenter Background The problem To bring a drug to the point where it can be used in the clinic, assessing the ability
Precision-tracking of individual particles By Fluorescence Photo activation Localization Microscopy(FPALM) Presented by Aung K.
Precision-tracking of individual particles By Fluorescence Photo activation Localization Microscopy(FPALM) Presented by Aung K. Soe This FPALM research was done by Assistant Professor Sam Hess, physics
Algorithms for Genetics: Basics of Wright Fisher Model and Coalescent Theory
Algorithms for Genetics: Basics of Wright Fisher Model and Coalescent Theory Vineet Bafna Harish Nagarajan and Nitin Udpa 1 Disclaimer Please note that a lot of the text and figures here are copied from
Colony Imaging with powerful Analysis Software
TM Imaging with powerful Analysis Software TM Accurate Compact Fast We re not going to interpret your results, but we ll do everything to get you there From image acquisition to data visualisation, straight
ULS24 is built on 0.18um CMOS process at a world-leader specialty semiconductor foundry. LDO. 3.3v/1.8v 30mW. SPI uc (32-bit) 12 Mhz OSC
Product overview The Anitoa ULS24 is an ultra-low-light CMOS image sensor. Its low cost, small form factor and high level of integration make it optimally suited for use in a portable device in medical,
Integrated Science II C0ourse #: SC-08 Grade Level: 8
C0ourse #: SC-08 Grade Level: 8 Course Name: Level of Difficulty: Average Prerequisites: See Counselor 1 year Strand 1: Inquiry Process s 1: 2: 3: 4: Science as inquiry is basic to science education and
INSTRUMENTATION BREADBOARDING (VERSION 1.3)
Instrumentation Breadboarding, Page 1 INSTRUMENTATION BREADBOARDING (VERSION 1.3) I. BACKGROUND The purpose of this experiment is to provide you with practical experience in building electronic circuits
MagSi Beads. Magnetic Silica beads. and In-Vitro Diagnostics
MagSi Beads Magnetic Silica beads for Research in Life Science and InVitro Diagnostics Wide range of products for numerous Applications AMS Biotechnology supplies a unique range of magnetic and nonmagnetic
Life of a Stanford Invention
Life of a Stanford Invention Notable Stanford Inventions Functional Antibodies FM Sound Synthesis Recombinant DNA Google Timeline of Stanford Inventions Big Winners 1970 OTL Established 1971 FM Sound Synthesis
Every human cell (except red blood cells and sperm and eggs) has an. identical set of 23 pairs of chromosomes which carry all the hereditary
Introduction to Genetic Genealogy Every human cell (except red blood cells and sperm and eggs) has an identical set of 23 pairs of chromosomes which carry all the hereditary information that is passed
Analysis Optimization Amphacademy 2018
Reinventing Single Cell Analysis Analysis Optimization Amphacademy 2018 Silvan Kaufmann Amphasys AG, Technopark Lucerne, 6039 Root D4, Switzerland Contents Introduction Gating strategies Advanced gate
U N I V E R S I T Y O F C A L I C U T Pareeksha Bhavan
U N I V E R S I T Y O F C A L I C U T Pareeksha Bhavan REVISED TIME TABLE FOR THE SEVENTH SEMESTER B.Tech/ P T B.Tech (2009 SCHEME) REGULAR / SUPPLEMENTARY / EXAMINATIONS NOVEMBER 2016 Time of Examination:
Implementation of HTRF. technology on the Spark. multimode reader SENSITIVE, FAST AND RELIABLE MEASUREMENT OF HIGH-THROUGHPUT SCREENING ASSAYS
Implementation of HTRF technology on the Spark multimode reader Application Note SENSITIVE, FAST AND RELIABLE MEASUREMENT OF HIGH-THROUGHPUT SCREENING ASSAYS 2 INTRODUCTION Homogeneous Time-Resolved Fluorescence
Find the Missing Links
From Eye to Insight Find the Missing Links DMi8 S Advanced Live Cell Imaging Solution DMi8 S Advanced Live Cell Imaging Solution 2 See what you ve been missing The DMi8 S is a complete solution for advanced
Lecture 10: Memetic Algorithms - I. An Introduction to Meta-Heuristics, Produced by Qiangfu Zhao (Since 2012), All rights reserved
Lecture 10: Memetic Algorithms - I Lec10/1 Contents Definition of memetic algorithms Definition of memetic evolution Hybrids that are not memetic algorithms 1 st order memetic algorithms 2 nd order memetic
Quick Guide. NucleoCounter NC-3000
Quick Guide NucleoCounter NC-3000 Table of contents Setting up the FlexiCyte Protocol 2 Editing Image Capture and Analysis Parameters 3 Optimizing Exposure Time 4 Compensation for Spectral Overlap 6 Creating
Bias and Power in the Estimation of a Maternal Family Variance Component in the Presence of Incomplete and Incorrect Pedigree Information
J. Dairy Sci. 84:944 950 American Dairy Science Association, 2001. Bias and Power in the Estimation of a Maternal Family Variance Component in the Presence of Incomplete and Incorrect Pedigree Information
FRANKENTOY What do you get when you mix and match animal parts?
FRANKENTOY What do you get when you mix and match animal parts? WHO WAS FRANKENSTEIN? What do you know about Victor Frankenstein and his creature? Victor Frankenstein and the monster he created were invented
Back to (the Article of) the Future?
Back to (the Article of) the Future? 4 September 2017 Hello. Maarten Cleeren Director of Product Management, Enriched Content Research Products at Elsevier I don t like the idea of an article-of-the
AFDAA 2012 WINTER MEETING Population Statistics Refresher Course - Lecture 3: Statistics of Kinship Analysis
AFDAA 2012 WINTER MEETING Population Statistics Refresher Course - Lecture 3: Statistics of Kinship Analysis Ranajit Chakraborty, PhD Center for Computational Genomics Institute of Applied Genetics Department
Are Scale-Free Networks Functionally Robust?
Are Scale-Free Networks Functionally Robust? Alon Keinan 1, Eytan Ruppin 1,2 1 School of Computer Sciences, Tel-Aviv University, Tel-Aviv, Israel {keinanak,ruppin}@post.tau.ac.il 2 School of Medicine,
Life of a Stanford Invention
Life of a Stanford Invention Notable Stanford Inventions Functional Antibodies FM Sound Synthesis Recombinant DNA Google Timeline of Stanford Inventions Big Winners 1970 OTL Established 1971 FM Sound Synthesis
[E-BOOK] HUMAN PEDIGREE GENETICS PROBLEM SET ANSWERS EBOOK
![[E-BOOK] HUMAN PEDIGREE GENETICS PROBLEM SET ANSWERS EBOOK](/thumbs/78/78801224.jpg "[E-BOOK] HUMAN PEDIGREE GENETICS PROBLEM SET ANSWERS EBOOK")
04 March, 2018 [E-BOOK] HUMAN PEDIGREE GENETICS PROBLEM SET ANSWERS EBOOK Document Filetype: PDF 393.35 KB 0 [E-BOOK] HUMAN PEDIGREE GENETICS PROBLEM SET ANSWERS EBOOK Problem set questions from Final
Health Implications from Mobile Communication Systems
COST 281 action Potential Health Implications from Mobile Communication Systems Report to COST-TIST TIST Maila Hietanen Vice Chair of COST 281 Progress Report Period: July 2003 June 2004 V/1 Cooperation
Genealogical trees, coalescent theory, and the analysis of genetic polymorphisms
Genealogical trees, coalescent theory, and the analysis of genetic polymorphisms Magnus Nordborg University of Southern California The importance of history Genetic polymorphism data represent the outcome
Prentice Hall Biology 2004 (Miller/Levine) Correlated to: Delaware Science Performance Indicators, Level Two (Grade 10)
Prentice Hall Biology 2004 (Miller/Levine) Delaware Science Performance Indicators, Level Two (Grade 10) LEVEL TWO: (GRADE 10) Chemical Reactions Chemical reactions involve the breaking and or forming
Prentice Hall Biology: Exploring Life 2004 Correlated to: Pennsylvania Academic Standards for Science and Technology (By the End of Grade 10)
Pennsylvania Academic Standards for Science and Technology (By the End of Grade 10) 3.1 UNIFYING THEMES 3.1.10. GRADE 10 A. Discriminate among the concepts of systems, subsystems, feedback and control
Photoacoustic Imaging of Blood Vessels in Tissue
of Blood Vessels in Tissue F.F.M. de Mul (University of Twente, Enschede, the Netherlands) FdM [µm] Imaging methods for hidden structures in turbid media (tissue) OCT/ OPS (C)M TOF / FM NIR green C(M)
Nature Protocols: doi: /nprot Supplementary Figure 1. Read quality score per sequenced base position.
Supplementary Figure 1 Read quality score per sequenced base position. (a) Illumina Q-scores for a normal QiSeq run. Typically, more than 80% of reads reach a quality score higher than 30 across all read
V SALAI SELVAM, AP & HOD, ECE, Sriram Engg. College, Perumalpattu 1 MEDICAL ELECTRONICS UNIT IV
V SALAI SELVAM, AP & HOD, ECE, Sriram Engg. College, Perumalpattu 1 MEDICAL ELECTRONICS UNIT IV Ionizing and non-ionizing radiations: The radiation that ionizes the gases through which it travels is known
Confocal Microscope. Confocal Microscope C2
Confocal Microscope Confocal Microscope C2 Confocal Microscope An essential microscopy laboratory instrument The C2 confocal microscope system comprises a new generation of Nikon confocal instruments designed
How do photoreceptors work?
How do photoreceptors work? Convert energy from light into nerve signals No easy feat!! 200 femtoseconds!! Phototransduction Isomerization Opsin - transducin Transducin - PDE PDE - cgmp to GMP Low cgmp
Developing Conclusions About Different Modes of Inheritance
Pedigree Analysis Introduction A pedigree is a diagram of family relationships that uses symbols to represent people and lines to represent genetic relationships. These diagrams make it easier to visualize
Cytation Cell Imaging Multi-Mode Reader
Cytation Cell Imaging Multi-Mode Reader An Imaging Primer I M A G I N G Cytation Cell Imaging Multi-Mode Reader An Imaging Primer Table of Contents 1. Introduction -----------------------------------------------------------------------------------------------------------------------------------------------------------
#P Quality Measures for Imaging-based Cellular Assays
Abstract #P1224 - Quality Measures for Imaging-based Cellular Assays Ilya Ravkin, Vitra Bioscience, Inc. Z-factor and related measures are useful in estimating assay variability in HTS caused by assay
Innate-like functions of natural killer T cell subsets result from highly divergent gene programs
CORRECTION NOTICE Nat. Immunol.; doi:1.13/ni.3437; corrected online 26 April 216 Innate-like functions of natural killer T cell subsets result from highly divergent gene programs Isaac Engel, Grégory Seumois,
Ratto Gian Michele. Summary report: Nanobiotechnology. + Publications + Curriculum vitae Gian Michele Ratto, Curriculum vitae
Ratto Gian Michele Education: 1984 Degree in Phsysics at the University of Genoa 1984-1988 University of California at Berkeley. Post doctoral fellow in the Department of Physiology and Biophysics with
Acute Lymphocytic Leukemia Detection and Classification (ALLDC) System
Acute Lymphocytic Leukemia Detection and Classification (ALLDC) System Jamila Harbi, PhD Computer Science Dept. College of Science Al- Mustansiriyah University Baghdad, Iraq Rana Ali Computer Science Dept.
Date: October 24, 2013 To:
E-11 From: To: cc: Commission Secretary BCUC:EX; Subject: Date: BC Hydro Smart Meter Opt-Out Program Thursday, October 24, 2013 3:51:37 PM Date: October 24, 2013 To: commission.secretary@bcuc.com Subject:
Bipolar Junction Transistor
ESE 211 / Spring 2011 / Lecture 10 Bipolar Junction Transistor Let us first consider general transconductance amplifier loaded with short circuit Transconductance Obviously, power supplies are needed for
Nature Methods: doi: /nmeth Supplementary Figure 1. Schematic of 2P-ISIM AO optical setup.
Supplementary Figure 1 Schematic of 2P-ISIM AO optical setup. Excitation from a femtosecond laser is passed through intensity control and shuttering optics (1/2 λ wave plate, polarizing beam splitting
Dec 2009 Breast Committee CALGB 40502
CALGB 40502/CTSU 40502 A Randomized Phase III Trial of Weekly Paclitaxel Compared To Weekly Nanoparticle Albumin Bound Nab- Paclitaxel or Ixabepilone Combined with Bevacizumab as First-Line Therapy for
ANEXOS A LA SOLICITUD DE DEPÓSITO DE LA NACIONAL DE LÍNEAS CELULARES ANNEXES TO THE APPLICATION FORM FOR DEPOSITING
ANEXOS A LA SOLICITUD DE DEPÓSITO DE LA LÍNEA CELULAR IC-AD2-F-iPS-4F-1 EN EL BANCO NACIONAL DE LÍNEAS CELULARES ANNEXES TO THE APPLICATION FORM FOR DEPOSITING IC-AD2-F-iPS-4F-1 CELL LINE INTO THE NATIONAL
Entire Chromosome 21 Inherited from 5 th Great Grandparents Captain Daniel and Elizabeth (Windecker) Young
Entire Chromosome 21 Inherited from 5 th Great Grandparents Captain Daniel and Elizabeth (Windecker) Young The Ancestors: Daniel Young was born about 1755 in the Canajoharie District of the Mohawk Valley
Photoacoustic imaging using an 8-beam Fabry-Perot scanner
Photoacoustic imaging using an 8-beam Fabry-Perot scanner Nam Huynh, Olumide Ogunlade, Edward Zhang, Ben Cox, Paul Beard Department of Medical Physics and Biomedical Engineering, University College London,
Greedy Algorithms and Genome Rearrangements
Greedy Algorithms and Genome Rearrangements 1. Transforming Cabbage into Turnip 2. Genome Rearrangements 3. Sorting By Reversals 4. Pancake Flipping Problem 5. Greedy Algorithm for Sorting by Reversals
Nature Methods: doi: /nmeth Supplementary Figure 1. sospim principle and representation of the sospim beam-steering unit.
Supplementary Figure 1 sospim principle and representation of the sospim beam-steering unit. Schematic representation of the sospim principle showing a sample holder comprising 45 micromirrored cavities
Find JCD Project Date: Identification-DNA Process Updated:
New Look Investigations Created by: Jack Friess Find JCD Project Date: 04-20-2018 Identification-DNA Process Updated: 05-24-2018 Questions and Answers Identification-DNA (ID-DNA) is a scientific process
Final Performance Report. Multifunctional Magnetic Nanowires for Biomagnetic Interfacing Concepts
Final Performance Report Multifunctional Magnetic Nanowires for Biomagnetic Interfacing Concepts AFOSR Agreement Number F49620-02-1-0307 July 14, 2006 Reporting Period: June 15, 2002 December 15, 2005