On the variability of transverse elastic properties of P. pinaster at the cellular level

Size: px
Start display at page:

Download "On the variability of transverse elastic properties of P. pinaster at the cellular level"

Transcription

1 COST Action FP0802 Thematic workshop: Mixed numerical and experimental methods applied to the mechanical characterization of bio based materials On the variability of transverse elastic properties of P. pinaster at the cellular level João Pereira, José Xavier, Pedro Couto, José Morais, José Lousada, Pedro Melo Pinto April 27 28, 2011 Vila Real, Portugal

2 OUTLINE INTRODUCTION ANNUAL RING LEVEL - X-Ray microdensitometry - Cellular geometrical characterisation CONCLUSIONS & OUTLOOK 2

3 INTRODUCTION Wood material why wood? - Important forest resources on Earth - Renewable/Recyclable - Important engineering material - Has good stiffness/weight ratio why Pinus pinaster? - Important Portuguese natural resource - Traditional structural material - Important raw-material for carpentry and furniture why RT plane? - Lack of mechanical characterisation - Need better understanding of structural/mechanical relationships 3

4 INTRODUCTION Scale of observation: Annual ring level - Earlywood(EW)/latewood(LW) heterogeneity - Cellular geometrical characterisation 4

5 ANNUAL RING LEVEL: X-Ray microdensitometry Material and methods Wood samples: 120(R)x5(L)x5(T) (mm) Microdensitometer (spatial resolution = 0.1 mm) X-Ray chamber X-Ray film (X-Ray film: optical density) 5

6 ANNUAL RING LEVEL: X-Ray microdensitometry Density profile Density (g.cm-3) Width (mm) % Ring average earlywood latewood ring earlywood latewood Latewood Heterogeneity index AR AR

7 Cellular model Regular hexagon model (adapted from Thuvander et al, 2000) Structure/elastic properties relationships: 7

8 Proposed work How the geometrical parameters of the cell model (t, h, d1, d2, l, q) vary within individual annual ring and radially along the stem of the tree? Consequently, how the transverse elastic properties of P. pinaster vary within individual annual ring and radially along the stem of the tree? 8

9 Material and methods Sample preparation (wood slices) Wood specimens: 15(R)x10(L)x8(T) (mm) Autoclave Sliding knife microtome Entellan 9

10 Material and methods Image acquisition Optical fluorescence microscopy (Olympus BX 50 at 200x magnification) AVT Dolphin F-201C digital camera (1280x960 pixel; conv. factor = 0.46 mm/pixel) 10

11 Material and methods Image processing tools Gimp ; Matlab Adjacent images overlapping: Annual ring reconstruction 11

12 Drawing CAD tools: AutoCAD R10 Earlywood drawing maps Cellular structure mesh Net maps (cell geometry) Boundary maps (lumen geometry) 12

13 Drawing CAD tools Earlywood measurements 13

14 Drawing CAD tools Latewood drawing maps Cellular structure mesh Net maps (cell geometry) Boundary maps (lumen geometry) 14

15 Drawing CAD tools Latewood measurements 15

16 Results AR10 AR41 16

17 Results AR10 AR41 17

18 Results AR10 AR41 18

19 Results AR10 AR41 19

20 Results AR10 AR41 20

21 Results AR10 AR41 21

22 Estimation of elastic properties: Earlywood 18.92% 48.61% 67.22% 16.86% 22

23 CONCLUSIONS & OUTLOOK CONCLUSIONS - Cellular geometrical parameters were identified for P. pinaster wood within annual ring and along the stem, using CAD tools; - The radial variation of transverse elastic properties was estimated from the cellular geometrical parameters based on hexagonal unit cell model. The trend of variation indicates an increase of elastic properties from the innermost to the outermost of the stem. 23

24 CONCLUSIONS & OUTLOOK Simple segmentation Edge detection Multiple edge detection Multiple segmentation OUTLOOK - Enhance automatic image processing and analyses for geometrical parameters characterisation; Boundary maps (lumen geometry) 24

25 CONCLUSIONS & OUTLOOK OUTLOOK - Assessing the radial variation of transverse elastic properties by mechanical testing at the annual ring scale. 25

26 CONCLUSIONS & OUTLOOK Thank you João L. Pereira Wood Engineering Dpt. at Viseu High School of Technology (PhD Student at UTAD) Special thanks to Professors: José Xavier; José Morais; José Lousada 26

ON THE ESTIMATION OF ELASTIC PROPERTIES OF WOOD AT THE GROWTH RING SCALE

ON THE ESTIMATION OF ELASTIC PROPERTIES OF WOOD AT THE GROWTH RING SCALE ON THE ESTIMATION OF ELASTIC PROPERTIES OF WOOD AT THE GROWTH RING SCALE J. Xavier, J. Pereira, F. Pierron, J. Morais, J. Lousada CITAB/UTAD, Vila Real, Portugal LMPF/ENSAM, Châlons-en-Champagne, France

More information

ON THE EVALUATION OF ELASTIC PROPERTIES OF P. PINASTER AT THE GROWTH RING SCALE

ON THE EVALUATION OF ELASTIC PROPERTIES OF P. PINASTER AT THE GROWTH RING SCALE evista da Associação Portuguesa de Análise xperimental de Tensões ISSN 1646-7078 ON TH VALUATION OF LASTIC POPTIS OF P. PINAST AT TH GOWTH ING SCAL Pereira, J. 1,2 ; Xavier, J. 1 ; Morais, J. 1 ; Lousada,

More information

Studying of Reflected Light Optical Laser Microscope Images Using Image Processing Algorithm

Studying of Reflected Light Optical Laser Microscope Images Using Image Processing Algorithm IRAQI JOURNAL OF APPLIED PHYSICS Fatema H. Rajab Al-Nahrain University, College of Engineering, Department of Laser and Optoelectronic Engineering Studying of Reflected Light Optical Laser Microscope Images

More information

Bioimage Informatics

Bioimage Informatics Bioimage Informatics Lecture 5, Spring 01 Fundamentals of Fluorescence Microscopy (II) Bioimage Data Analysis (I): Basic Operations Lecture 5 January 5, 01 1 Outline Performance metrics of a microscope

More information

A Numerical Study of the Transverse Modulus of Wood as a Function of Grain Orientation and Properties

A Numerical Study of the Transverse Modulus of Wood as a Function of Grain Orientation and Properties Holzforschung, in press (6) A Numerical Study of the Transverse Modulus of Wood as a Function of Grain Orientation and Properties By J. A. Nairn 1 Wood Science & Engineering, Oregon State University, Corvallis,

More information

Multi-resolution Cervical Cell Dataset

Multi-resolution Cervical Cell Dataset Report 37 Multi-resolution Cervical Cell Dataset Patrik Malm December 2013 Centre for Image Analysis Swedish University of Agricultural Sciences Uppsala University Uppsala 2013 Multi-resolution Cervical

More information

Fast, high-contrast imaging of animal development with scanned light sheet based structured-illumination microscopy

Fast, high-contrast imaging of animal development with scanned light sheet based structured-illumination microscopy nature methods Fast, high-contrast imaging of animal development with scanned light sheet based structured-illumination microscopy Philipp J Keller, Annette D Schmidt, Anthony Santella, Khaled Khairy,

More information

Digital Camera Technologies for Scientific Bio-Imaging. Part 2: Sampling and Signal

Digital Camera Technologies for Scientific Bio-Imaging. Part 2: Sampling and Signal Digital Camera Technologies for Scientific Bio-Imaging. Part 2: Sampling and Signal Yashvinder Sabharwal, 1 James Joubert 2 and Deepak Sharma 2 1. Solexis Advisors LLC, Austin, TX, USA 2. Photometrics

More information

WinDENDRO An Image Analysis System For Tree-Ring Analysis

WinDENDRO An Image Analysis System For Tree-Ring Analysis www.regentinstruments.com sales@regentinstruments.com WinDENDRO 2017 An Image Analysis System For Tree-Ring Analysis WinDENDRO has been specifically designed for dendrometrists and dendrochronologists

More information

Wood structure I: Basic features, structure and cell types

Wood structure I: Basic features, structure and cell types CHEM-E0120: An Introduction to Wood Properties and Wood Products Wood structure I: Basic features, structure and cell types Mark Hughes 18 th September 2017 Today Making trees: photosynthesis Tree types

More information

Finite Element Analyses of Two Dimensional, Anisotropic Heat Transfer in Wood

Finite Element Analyses of Two Dimensional, Anisotropic Heat Transfer in Wood Finite Element Analyses of Two Dimensional, Anisotropic Heat Transfer in Wood John F. Hunt Hongmei Gu USDA, Forest Products Laboratory One Gifford Pinchot Drive Madison, WI 53726 Abstract The anisotropy

More information

Single-photon excitation of morphology dependent resonance

Single-photon excitation of morphology dependent resonance Single-photon excitation of morphology dependent resonance 3.1 Introduction The examination of morphology dependent resonance (MDR) has been of considerable importance to many fields in optical science.

More information

Distributions of Tracheid Cross-Sectional Dimensions in Different Parts of Norway Spruce Stems

Distributions of Tracheid Cross-Sectional Dimensions in Different Parts of Norway Spruce Stems Silva Fennica 42(1) research articles www.metla.fi/silvafennica ISSN 0037-5330 The Finnish Society of Forest Science The Finnish Forest Research Institute Distributions of Tracheid Cross-Sectional Dimensions

More information

BEARING PROPERTIES OF PORTUGUESE PINE WOOD BENEATH A LATERALLY LOADED DOWEL

BEARING PROPERTIES OF PORTUGUESE PINE WOOD BENEATH A LATERALLY LOADED DOWEL BEARING PROPERTIES OF PORTUGUESE PINE WOOD BENEATH A LATERALLY LOADED DOWEL C.L. SANTOS*, A.M.P. de JESUS**, J.J.L. MORAIS*, J.L.P.C. LOUSADA*** *CITAB/Department of Engineering, **Department of Engineering,

More information

Bringing Answers to the Surface

Bringing Answers to the Surface 3D Bringing Answers to the Surface 1 Expanding the Boundaries of Laser Microscopy Measurements and images you can count on. Every time. LEXT OLS4100 Widely used in quality control, research, and development

More information

CHARACTERIZATION OF THE INTERNAL MICROSTRUCTURES OF GRANULAR MATERIALS USING COMPUTERIZED TOMOGRAPHY

CHARACTERIZATION OF THE INTERNAL MICROSTRUCTURES OF GRANULAR MATERIALS USING COMPUTERIZED TOMOGRAPHY CHARACTERIZATION OF THE INTERNAL MICROSTRUCTURES OF GRANULAR MATERIALS USING COMPUTERIZED TOMOGRAPHY Xiaogong Lee Apylied Research Associates, Inc. P.O. Box 40128 Tyndall AFB, FL 32403 William C. Dass

More information

Chapter 18 Optical Elements

Chapter 18 Optical Elements Chapter 18 Optical Elements GOALS When you have mastered the content of this chapter, you will be able to achieve the following goals: Definitions Define each of the following terms and use it in an operational

More information

COMPRESSION WOOD IN WESTERN HEMLOCK TSUGA HETEROPHYLLA (RAF.) SARG.' Somkid Siripatanadilok. and Lawrence Leney

COMPRESSION WOOD IN WESTERN HEMLOCK TSUGA HETEROPHYLLA (RAF.) SARG.' Somkid Siripatanadilok. and Lawrence Leney COMPRESSION WOOD IN WESTERN HEMLOCK TSUGA HETEROPHYLLA (RAF.) SARG.' Somkid Siripatanadilok Instructor Faculty of Forestry, Kasetsart University Bangkok 10903, Thailand and Lawrence Leney Professor Emeritus

More information

(Refer Slide Time: 00:10)

(Refer Slide Time: 00:10) Fundamentals of optical and scanning electron microscopy Dr. S. Sankaran Department of Metallurgical and Materials Engineering Indian Institute of Technology, Madras Module 03 Unit-6 Instrumental details

More information

A 3D Multi-Aperture Image Sensor Architecture

A 3D Multi-Aperture Image Sensor Architecture A 3D Multi-Aperture Image Sensor Architecture Keith Fife, Abbas El Gamal and H.-S. Philip Wong Department of Electrical Engineering Stanford University Outline Multi-Aperture system overview Sensor architecture

More information

Light Microscopy. Upon completion of this lecture, the student should be able to:

Light Microscopy. Upon completion of this lecture, the student should be able to: Light Light microscopy is based on the interaction of light and tissue components and can be used to study tissue features. Upon completion of this lecture, the student should be able to: 1- Explain the

More information

TESTFIELD TRENTO: GEOMETRIC EVALUATION OF VERY HIGH RESOLUTION SATELLITE IMAGERY

TESTFIELD TRENTO: GEOMETRIC EVALUATION OF VERY HIGH RESOLUTION SATELLITE IMAGERY TESTFIELD TRENTO: GEOMETRIC EVALUATION OF VERY HIGH RESOLUTION SATELLITE IMAGERY G. AGUGIAROa, D. POLIb, F. REMONDINOa, 3DOM, 3D Optical Metrology Unit Bruno Kessler Foundation, Trento, Italy a b Vermessung

More information

Dynamic Stability Characteristics of HSP-CM at Mach 4

Dynamic Stability Characteristics of HSP-CM at Mach 4 Dynamic Stability Characteristics of HSP-CM at Mach 4 Presentation at MATLAB EXPO India, 2017 20.04.2017 By, Aaron Baptista, Sci/Engr Akhtedar Abbas Khan, Sci/Engr MD Jamal Nawaz Ansari, SCI/Engr R Saravanan,

More information

INTRODUCTION THIN LENSES. Introduction. given by the paraxial refraction equation derived last lecture: Thin lenses (19.1) = 1. Double-lens systems

INTRODUCTION THIN LENSES. Introduction. given by the paraxial refraction equation derived last lecture: Thin lenses (19.1) = 1. Double-lens systems Chapter 9 OPTICAL INSTRUMENTS Introduction Thin lenses Double-lens systems Aberrations Camera Human eye Compound microscope Summary INTRODUCTION Knowledge of geometrical optics, diffraction and interference,

More information

Advanced 3D Optical Profiler using Grasshopper3 USB3 Vision camera

Advanced 3D Optical Profiler using Grasshopper3 USB3 Vision camera Advanced 3D Optical Profiler using Grasshopper3 USB3 Vision camera Figure 1. The Zeta-20 uses the Grasshopper3 and produces true color 3D optical images with multi mode optics technology 3D optical profiling

More information

SilviScan measurements on Maritime pine

SilviScan measurements on Maritime pine SilviScan measurements on Maritime pine French samples cut perpendicular to the fibres Sven-Olof Lundqvist, Åke Hansson, Lars Olsson STFI-Packforsk report no.: 326 November 27 Distribution restricted to:

More information

Very short introduction to light microscopy and digital imaging

Very short introduction to light microscopy and digital imaging Very short introduction to light microscopy and digital imaging Hernan G. Garcia August 1, 2005 1 Light Microscopy Basics In this section we will briefly describe the basic principles of operation and

More information

PATTERN OF VARIATION OF FIBRIL ANGLE WITHIN ANNUAL RINGS OF PINUS ATTENURADIATA

PATTERN OF VARIATION OF FIBRIL ANGLE WITHIN ANNUAL RINGS OF PINUS ATTENURADIATA UNITED STATES DEPARTMENT OF AGRICULTURE. FOREST SERVICE. FOREST PRODUCTS LABORATORY. MADISON, WIS PATTERN OF VARIATION OF FIBRIL ANGLE WITHIN ANNUAL RINGS OF PINUS ATTENURADIATA FPL-034 April 1964 PATTERN

More information

EPIFLUORESCENCE &/OR BRIGHTFIELD MICROSCOPY

EPIFLUORESCENCE &/OR BRIGHTFIELD MICROSCOPY EPIFLUORESCENCE &/OR BRIGHTFIELD MICROSCOPY TURN ON THE FOLLOWING EQUIPMENT The fluorescent light (if needed) The power strip for the microscope and accessories The CoolSNAP HQ camera on the right (Turn

More information

This report can be found on the Web at

This report can be found on the Web at Auburn University is an equal opportunity educational institution/employee. Information contained herein is available to all persons without regard to race, color, sex, or national origin. http:www.auburn.edu

More information

A numerical study of the transverse modulus of wood as a function of grain orientation and properties

A numerical study of the transverse modulus of wood as a function of grain orientation and properties Holzforschung, Vol. 61, pp. 406 413, 2007 Copyright by Walter de Gruyter Berlin New York. DOI 10.1515/HF.2007.079 A numerical study of the transverse modulus of wood as a function of grain orientation

More information

Physics 3340 Spring Fourier Optics

Physics 3340 Spring Fourier Optics Physics 3340 Spring 011 Purpose Fourier Optics In this experiment we will show how the Fraunhofer diffraction pattern or spatial Fourier transform of an object can be observed within an optical system.

More information

MINIATURE X-RAY SOURCES AND THE EFFECTS OF SPOT SIZE ON SYSTEM PERFORMANCE

MINIATURE X-RAY SOURCES AND THE EFFECTS OF SPOT SIZE ON SYSTEM PERFORMANCE 228 MINIATURE X-RAY SOURCES AND THE EFFECTS OF SPOT SIZE ON SYSTEM PERFORMANCE D. CARUSO, M. DINSMORE TWX LLC, CONCORD, MA 01742 S. CORNABY MOXTEK, OREM, UT 84057 ABSTRACT Miniature x-ray sources present

More information

Nikon. King s College London. Imaging Centre. N-SIM guide NIKON IMAGING KING S COLLEGE LONDON

Nikon. King s College London. Imaging Centre. N-SIM guide NIKON IMAGING KING S COLLEGE LONDON N-SIM guide NIKON IMAGING CENTRE @ KING S COLLEGE LONDON Starting-up / Shut-down The NSIM hardware is calibrated after system warm-up occurs. It is recommended that you turn-on the system for at least

More information

coating pigments into the base serial sectioning

coating pigments into the base serial sectioning n of the penetration of coating pigments into the base paper determined by automated serial sectioning J. Kritzinger, W. Bauer, P. Hunziker and M. Kässberger PaperCon 2011 Page 552 Coating Penetration

More information

QUASI-STATIC MECHANICAL BEHAVIOR OF A DOUBLE-SHEAR SINGLE DOWEL WOOD CONNECTION

QUASI-STATIC MECHANICAL BEHAVIOR OF A DOUBLE-SHEAR SINGLE DOWEL WOOD CONNECTION Proceedings of COBEM 2007 Copyright 2007 by ABCM 19th International Congress of Mechanical Engineering November 5-9, 2007, Brasília, DF QUASI-STATIC MECHANICAL BEHAVIOR OF A DOUBLE-SHEAR SINGLE DOWEL WOOD

More information

Low-energy Electron Diffractive Imaging for Three dimensional Light-element Materials

Low-energy Electron Diffractive Imaging for Three dimensional Light-element Materials Low-energy Electron Diffractive Imaging for Three dimensional Light-element Materials Hitachi Review Vol. 61 (2012), No. 6 269 Osamu Kamimura, Ph. D. Takashi Dobashi OVERVIEW: Hitachi has been developing

More information

Wettability of weathered wood

Wettability of weathered wood J. Adhension Sci. Technol. Vol. 6, No. 12, pp. 1325-1330 (1992) VSP 1992. Wettability of weathered wood MARTINS A. KALNINS* AND MARK T. KNAEBE USDA Forest Service, Forest Products Laboratory, One Gifford

More information

Hardness distribution on wood surface

Hardness distribution on wood surface J Wood Sei (21) 47:1-7 9 The Japan Wood Research Society 21 Seiji Hirata 9 Masamitsu Ohta 9 Yasuo Homna Hardness distribution on wood surface Received: September 14, 1999 / Accepted: January 28, 2 Abstract

More information

Woodna, within its walnut surfaces workline ( launches Woodna Oblicua Geométrica, an innovative, high-performance, versatile product

Woodna, within its walnut surfaces workline (  launches Woodna Oblicua Geométrica, an innovative, high-performance, versatile product Woodna, within its walnut surfaces workline (www.woodna.es), launches Woodna Oblicua Geométrica, an innovative, high-performance, versatile product with many possibilities for designers. Woodna owns 1300

More information

Education in Microscopy and Digital Imaging

Education in Microscopy and Digital Imaging Contact Us Carl Zeiss Education in Microscopy and Digital Imaging ZEISS Home Products Solutions Support Online Shop ZEISS International ZEISS Campus Home Interactive Tutorials Basic Microscopy Spectral

More information

Compatible with Windows 8/7/XP, and Linux; Universal programming interfaces for easy custom programming.

Compatible with Windows 8/7/XP, and Linux; Universal programming interfaces for easy custom programming. NIRvana: 640LN The NIRvana: 640LN from Princeton Instruments is a scientific-grade, deep-cooled, large format InGaAs camera for low-light scientific SWIR imaging and spectroscopy applications. The camera

More information

Operation Guide for the Leica SP2 Confocal Microscope Bio-Imaging Facility Hunter College October 2009

Operation Guide for the Leica SP2 Confocal Microscope Bio-Imaging Facility Hunter College October 2009 Operation Guide for the Leica SP2 Confocal Microscope Bio-Imaging Facility Hunter College October 2009 Introduction of Fluoresence Confocal Microscopy The first confocal microscope was invented by Princeton

More information

Practical work no. 3: Confocal Live Cell Microscopy

Practical work no. 3: Confocal Live Cell Microscopy Practical work no. 3: Confocal Live Cell Microscopy Course Instructor: Mikko Liljeström (MIU) 1 Background Confocal microscopy: The main idea behind confocality is that it suppresses the signal outside

More information

ELECTRON MICROSCOPY. 13:10 16:00, Oct. 6, 2008 Institute of Physics, Academia Sinica. Tung Hsu

ELECTRON MICROSCOPY. 13:10 16:00, Oct. 6, 2008 Institute of Physics, Academia Sinica. Tung Hsu ELECTRON MICROSCOPY 13:10 16:00, Oct. 6, 2008 Institute of Physics, Academia Sinica Tung Hsu Department of Materials Science and Engineering National Tsing Hua University Hsinchu 300, TAIWAN Tel. 03-5742564

More information

WOOD GOOD GOODWOOD.SX LUMBER PROFILE

WOOD GOOD GOODWOOD.SX LUMBER PROFILE GOOD WOOD LUMBER PROFILE GOODWOOD.SX WHY GOOD WOOD? BEST SUITED FOR THE CARIBBEAN CLIMATE USED FOR GENERATIONS FOR THE CONSTRUCTION OF HOUSES, BOATS, FURNITURE, ETC. NATURAL RESISTANCE AGAINST TERMITES

More information

In-Vivo IMAGING SYSTEMS. A complete line of high resolution optical & X-ray systems for pre-clinical imaging

In-Vivo IMAGING SYSTEMS. A complete line of high resolution optical & X-ray systems for pre-clinical imaging In-Vivo IMAGING SYSTEMS A complete line of high resolution optical & X-ray systems for pre-clinical imaging In-Vivo Imaging Systems Carestream is a strong, successful, multi-billion dollar, international

More information

LSM 800 Confocal Microscope Standard Operation Protocol

LSM 800 Confocal Microscope Standard Operation Protocol LSM 800 Confocal Microscope Standard Operation Protocol Turning on the system 1. Switch on the Main switch (labeled 1 and 2 ) mounted on the wall. 2. Turn the Laser Key (labeled 3 ) 90 clockwise for power

More information

Overview. Pinhole camera model Projective geometry Vanishing points and lines Projection matrix Cameras with Lenses Color Digital image

Overview. Pinhole camera model Projective geometry Vanishing points and lines Projection matrix Cameras with Lenses Color Digital image Camera & Color Overview Pinhole camera model Projective geometry Vanishing points and lines Projection matrix Cameras with Lenses Color Digital image Book: Hartley 6.1, Szeliski 2.1.5, 2.2, 2.3 The trip

More information

FLUORESCENCE MICROSCOPY. Matyas Molnar and Dirk Pacholsky

FLUORESCENCE MICROSCOPY. Matyas Molnar and Dirk Pacholsky FLUORESCENCE MICROSCOPY Matyas Molnar and Dirk Pacholsky 1 The human eye perceives app. 400-700 nm; best at around 500 nm (green) Has a general resolution down to150-300 μm (human hair: 40-250 μm) We need

More information

Image analysis. Intensity measurements Size measurements Organelle localization Colocalization Cell mobility Distance measurements FRAP, FLIP, FRET

Image analysis. Intensity measurements Size measurements Organelle localization Colocalization Cell mobility Distance measurements FRAP, FLIP, FRET Dr. Kees Straatman Image analysis Imaris Volocity ImageJ/Fiji Huygens deconvolution NIS-Elements (incl. deconvolution in RKCSB) ScanR analysis CellR analysis Cell Profiler FV1000/LAS Image analysis Intensity

More information

ELE 882: Introduction to Digital Image Processing (DIP)

ELE 882: Introduction to Digital Image Processing (DIP) ELE882 Introduction to Digital Image Processing Course Instructor: Prof. Ling Guan Department of Electrical & Computer Engineering Room 315, ENG Building Tel: (416)979-5000 ext 6072 Email: lguan@ee.ryerson.ca

More information

Water-Window Microscope Based on Nitrogen Plasma Capillary Discharge Source

Water-Window Microscope Based on Nitrogen Plasma Capillary Discharge Source 2015 International Workshop on EUV and Soft X-Ray Sources Water-Window Microscope Based on Nitrogen Plasma Capillary Discharge Source T. Parkman 1, M. F. Nawaz 2, M. Nevrkla 2, M. Vrbova 1, A. Jancarek

More information

Near-Infrared Spectral Imaging for the Visualization of Moisture Distribution inside Soybean Seeds

Near-Infrared Spectral Imaging for the Visualization of Moisture Distribution inside Soybean Seeds 1/ 31 Near-Infrared Spectral Imaging for the Visualization of Moisture Distribution inside Soybean Seeds M. Tsuta 1, Y. Ogawa 2, Y. Sagara 3, G. Do 4 and J. Sugiyama 1 1 National Food Research Institute,

More information

Two Dimensional Finite Element Heat Transfer Models for Softwood. Hongmei Gu 1. John F. Hunt, P.E. 2

Two Dimensional Finite Element Heat Transfer Models for Softwood. Hongmei Gu 1. John F. Hunt, P.E. 2 Two Dimensional Finite Element Heat Transfer Models for Softwood Hongmei Gu 1 John F. Hunt, P.E. 2 1 Post Doctorate Research Associate, hgu@fs.fed.us 2 Research Mechanical Engineer, jfhunt@fs.fed.us USDA

More information

Life Science Instrumentation. New Generation. Light Sheet Fluorescence Microscope. Alph

Life Science Instrumentation. New Generation. Light Sheet Fluorescence Microscope. Alph Life Science Instrumentation Light Sheet Fluorescence Microscope New Generation Alph Modular Light Sheet Microscope Alpha 3 is a new generation of light sheet fluorescence microscope addressing the needs

More information

Quick Guide for Zeiss 710 Laser Scanning Confocal MGH Cancer Center

Quick Guide for Zeiss 710 Laser Scanning Confocal MGH Cancer Center Quick Guide for Zeiss 710 Laser Scanning Confocal MGH Cancer Center For any questions or concerns, please contact: Linda Nieman lnieman@mgh.harvard.edu Office: (617) 643-9684 Cell: (512) 565-8076 Chenyue

More information

More fancy SPIM, Even fancier SPIM

More fancy SPIM, Even fancier SPIM More fancy SPIM, Even fancier SPIM Last class Light sheet microscopy Fancy SPIM (ispim, dspim, etc ) This class Multi camera SPIM SIM SPIM Bessels d x,y = λ em 2 NA d z = 2 NA λ ex + n(1 cosθ λ em 1 IsoView

More information

Holography. Casey Soileau Physics 173 Professor David Kleinfeld UCSD Spring 2011 June 9 th, 2011

Holography. Casey Soileau Physics 173 Professor David Kleinfeld UCSD Spring 2011 June 9 th, 2011 Holography Casey Soileau Physics 173 Professor David Kleinfeld UCSD Spring 2011 June 9 th, 2011 I. Introduction Holography is the technique to produce a 3dimentional image of a recording, hologram. In

More information

Axioscan - Startup. 1. Turn on the Axioscan on (button to the left) and turn on the computer

Axioscan - Startup. 1. Turn on the Axioscan on (button to the left) and turn on the computer Axioscan - Startup 1. Turn on the Axioscan on (button to the left) and turn on the computer 2. Log on and start the ZEN Blue software from the desktop 3. Press ZEN slidescan and Start System 4. Start by

More information

Illumination Correction tutorial

Illumination Correction tutorial Illumination Correction tutorial I. Introduction The Correct Illumination Calculate and Correct Illumination Apply modules are intended to compensate for the non uniformities in illumination often present

More information

Beacon Island Report / Notes

Beacon Island Report / Notes Beacon Island Report / Notes Paul Bourke, ivec@uwa, 17 February 2014 During my 2013 and 2014 visits to Beacon Island four general digital asset categories were acquired, they were: high resolution panoramic

More information

Application of Automatic Image Analysis to Wood Science

Application of Automatic Image Analysis to Wood Science Application of Automatic Image Analysis to Wood Science Charles W. McMillin ABSTRACT. In this paper I describe an image analysis system and illustl'ate with examples the application of automatic quantitative

More information

Wood structure II: Anatomy and properties

Wood structure II: Anatomy and properties CHEM-E0120: An Introduction to Wood Properties and Wood Products Wood structure II: Anatomy and properties Mark Hughes 21 st September 2017 Today The relationship between the technical properties of wood

More information

Electron-Bombarded CMOS

Electron-Bombarded CMOS New Megapixel Single Photon Position Sensitive HPD: Electron-Bombarded CMOS University of Lyon / CNRS-IN2P3 in collaboration with J. Baudot, E. Chabanat, P. Depasse, W. Dulinski, N. Estre, M. Winter N56:

More information

Hamidreza Karbasi, P. Eng., PhD Conestoga College ITAL Oct. 7, 2010

Hamidreza Karbasi, P. Eng., PhD Conestoga College ITAL Oct. 7, 2010 Presented at the COMSOL Conference 2010 Boston Presented by: Hamidreza Karbasi, P. Eng., PhD Conestoga College ITAL Oct. 7, 2010 Creating and Building Sustainable Environments Outline Background Objectives

More information

Working Simultaneously. The Next Level of TIRF Microscopy. cell^tirf Illuminator Motorized Total Internal Reflection Fluorescence

Working Simultaneously. The Next Level of TIRF Microscopy. cell^tirf Illuminator Motorized Total Internal Reflection Fluorescence cell^tirf Illuminator Motorized Total Internal Reflection Fluorescence Four individually aligned illumination beams for simultaneous multi-color TIRF imaging Working Simultaneously The Next Level of TIRF

More information

User manual for Olympus SD-OSR spinning disk confocal microscope

User manual for Olympus SD-OSR spinning disk confocal microscope User manual for Olympus SD-OSR spinning disk confocal microscope Ved Prakash, PhD. Research imaging specialist Imaging & histology core University of Texas, Dallas ved.prakash@utdallas.edu Once you open

More information

Shaping light in microscopy:

Shaping light in microscopy: Shaping light in microscopy: Adaptive optical methods and nonconventional beam shapes for enhanced imaging Martí Duocastella planet detector detector sample sample Aberrated wavefront Beamsplitter Adaptive

More information

SUPPLEMENTAL MATERIAL

SUPPLEMENTAL MATERIAL SUPPLEMENTAL MATERIAL 1 - Folios and areas of analysis Figure S1.1. Folio 4, areas of analysis for microxrf ( ), FORS ( ), micro-samples for Raman and FTIR ( ) and Raman in-situ ( ). Figure S1.2. Folio

More information

OIW-Microscopy. 13 September Connor Douglas PhD

OIW-Microscopy. 13 September Connor Douglas PhD OIW-Microscopy 13 September 2011 Connor Douglas PhD Optical System High Speed 2 mega-pixel CCD Camera Flow rate 30 litres per minute 10x lens magnification gives an image region of 650 x 494um Minimum

More information

Large Field of View, High Spatial Resolution, Surface Measurements

Large Field of View, High Spatial Resolution, Surface Measurements Large Field of View, High Spatial Resolution, Surface Measurements James C. Wyant and Joanna Schmit WYKO Corporation, 2650 E. Elvira Road Tucson, Arizona 85706, USA jcwyant@wyko.com and jschmit@wyko.com

More information

DICOM Correction Proposal

DICOM Correction Proposal Tracking Information - Administration Use Only DICOM Correction Proposal Correction Proposal Number Status CP-1713 Letter Ballot Date of Last Update 2018/01/23 Person Assigned Submitter Name David Clunie

More information

Be aware that there is no universal notation for the various quantities.

Be aware that there is no universal notation for the various quantities. Fourier Optics v2.4 Ray tracing is limited in its ability to describe optics because it ignores the wave properties of light. Diffraction is needed to explain image spatial resolution and contrast and

More information

Machining of Wood using a Rip Tooth: Effects of Work-piece Variations on Cutting Mechanics

Machining of Wood using a Rip Tooth: Effects of Work-piece Variations on Cutting Mechanics Machining of Wood using a Rip Tooth: Effects of Work-piece Variations on Cutting Mechanics Naylor, Andrew. 1* Hackney, Philip. 1 Clahr, Emil. 2 1 School of Computing, Engineering and Information Sciences,

More information

Acceptance test for the linear motion actuator for the scanning slit of the HIE ISOLDE short diagnostic boxes

Acceptance test for the linear motion actuator for the scanning slit of the HIE ISOLDE short diagnostic boxes EUROPEAN ORGANIZATION FOR NUCLEAR RESEARCH CERN ACC NOTE 2014 0099 HIE ISOLDE PROJECT Note 0036 Acceptance test for the linear motion actuator for the scanning slit of the HIE ISOLDE short diagnostic boxes

More information

ILLUMINATION AND IMAGE PROCESSING FOR REAL-TIME CONTROL OF DIRECTED ENERGY DEPOSITION ADDITIVE MANUFACTURING

ILLUMINATION AND IMAGE PROCESSING FOR REAL-TIME CONTROL OF DIRECTED ENERGY DEPOSITION ADDITIVE MANUFACTURING Solid Freeform Fabrication 2016: Proceedings of the 26th 27th Annual International Solid Freeform Fabrication Symposium An Additive Manufacturing Conference ILLUMINATION AND IMAGE PROCESSING FOR REAL-TIME

More information

LSM 710 Confocal Microscope Standard Operation Protocol

LSM 710 Confocal Microscope Standard Operation Protocol LSM 710 Confocal Microscope Standard Operation Protocol Basic Operation Turning on the system 1. Switch on Main power switch 2. Switch on System / PC power button 3. Switch on Components power button 4.

More information

Structured-Light Based Acquisition (Part 1)

Structured-Light Based Acquisition (Part 1) Structured-Light Based Acquisition (Part 1) CS635 Spring 2017 Daniel G. Aliaga Department of Computer Science Purdue University Passive vs. Active Acquisition Passive + Just take pictures + Does not intrude

More information

Supplementary Information

Supplementary Information Supplementary Information Simultaneous whole- animal 3D- imaging of neuronal activity using light field microscopy Robert Prevedel 1-3,10, Young- Gyu Yoon 4,5,10, Maximilian Hoffmann,1-3, Nikita Pak 5,6,

More information

Image and Multidimensional Signal Processing

Image and Multidimensional Signal Processing Image and Multidimensional Signal Processing Professor William Hoff Dept of Electrical Engineering &Computer Science http://inside.mines.edu/~whoff/ Digital Image Fundamentals 2 Digital Image Fundamentals

More information

Wood anatomy. 600 Wood anatomy

Wood anatomy. 600 Wood anatomy 600 Wood anatomy Wood anatomy Wood is composed mostly of hollow, elongated, Spindle-shaped cells that are arranged parallel to each other along the trunk of a tree. The characteristics of these fibrous

More information

Precision-tracking of individual particles By Fluorescence Photo activation Localization Microscopy(FPALM) Presented by Aung K.

Precision-tracking of individual particles By Fluorescence Photo activation Localization Microscopy(FPALM) Presented by Aung K. Precision-tracking of individual particles By Fluorescence Photo activation Localization Microscopy(FPALM) Presented by Aung K. Soe This FPALM research was done by Assistant Professor Sam Hess, physics

More information

Quick Guide for Zeiss 710 Laser Scanning Confocal MGH Cancer Center

Quick Guide for Zeiss 710 Laser Scanning Confocal MGH Cancer Center Quick Guide for Zeiss 710 Laser Scanning Confocal MGH Cancer Center For any questions or concerns, please contact: Linda Nieman lnieman@mgh.harvard.edu Office: (617) 643-9684 Cell: (512) 565-8076 Chenyue

More information

Last updated: May 2014 Y.DeGraaf

Last updated: May 2014 Y.DeGraaf FLINDERS MICROSCOPY BIOMEDICAL SERVICES AVAILABLE MICROSCOPES AND SPECIFICATIONS & INFORMATION REGARDING TRAINING FOR NEW USERS Last updated: May 2014 Y.DeGraaf If you have new staff or students (Honours/Masters

More information

Systems Biology. Optical Train, Köhler Illumination

Systems Biology. Optical Train, Köhler Illumination McGill University Life Sciences Complex Imaging Facility Systems Biology Microscopy Workshop Tuesday December 7 th, 2010 Simple Lenses, Transmitted Light Optical Train, Köhler Illumination What Does a

More information

Technology Integration Across Additive Manufacturing Domain to Enhance Student Classroom Involvement

Technology Integration Across Additive Manufacturing Domain to Enhance Student Classroom Involvement Paper ID #15500 Technology Integration Across Additive Manufacturing Domain to Enhance Student Classroom Involvement Prof. Tzu-Liang Bill Tseng, University of Texas - El Paso Dr. Tseng is a Professor and

More information

Technical information about PhoToPlan

Technical information about PhoToPlan Technical information about PhoToPlan The following pages shall give you a detailed overview of the possibilities using PhoToPlan. kubit GmbH Fiedlerstr. 36, 01307 Dresden, Germany Fon: +49 3 51/41 767

More information

Damage of the Cell Wall During Extrusion and Injection Molding of Wood Plastic Composites

Damage of the Cell Wall During Extrusion and Injection Molding of Wood Plastic Composites Damage of the Cell Wall During Extrusion and Injection Molding of Wood Plastic Composites William Gacitua E. 1* David F. Bahr 2 - Michael P. Wolcott 3 1 Assistant professor, Departamento Ingenieria en

More information

Microvasculature on a chip: study of the Endothelial Surface Layer and the flow structure of Red Blood Cells

Microvasculature on a chip: study of the Endothelial Surface Layer and the flow structure of Red Blood Cells Supplementary Information Microvasculature on a chip: study of the Endothelial Surface Layer and the flow structure of Red Blood Cells Daria Tsvirkun 1,2,5, Alexei Grichine 3,4, Alain Duperray 3,4, Chaouqi

More information

Boulevard du Temple Daguerrotype (Paris,1838) a busy street? Nyquist sampling for movement

Boulevard du Temple Daguerrotype (Paris,1838) a busy street? Nyquist sampling for movement Boulevard du Temple Daguerrotype (Paris,1838) a busy street? Nyquist sampling for movement CONFOCAL MICROSCOPY BioVis Uppsala, 2017 Jeremy Adler Matyas Molnar Dirk Pacholsky Widefield & Confocal Microscopy

More information

Light field sensing. Marc Levoy. Computer Science Department Stanford University

Light field sensing. Marc Levoy. Computer Science Department Stanford University Light field sensing Marc Levoy Computer Science Department Stanford University The scalar light field (in geometrical optics) Radiance as a function of position and direction in a static scene with fixed

More information

Electromagnetic, Thermal and Structural Analysis of the LUX Photoinjector Cavity using ANSYS. Steve Virostek Lawrence Berkeley National Lab

Electromagnetic, Thermal and Structural Analysis of the LUX Photoinjector Cavity using ANSYS. Steve Virostek Lawrence Berkeley National Lab Electromagnetic, Thermal and Structural Analysis of the LUX Photoinjector Cavity using ANSYS Steve Virostek Lawrence Berkeley National Lab 13 December 2004 Photoinjector Background The proposed LBNL LUX

More information

SILVA FENNICA. Tracheid Cross-sectional Dimensions in Scots Pine (Pinus sylvestris) Distributions and Comparison with Norway Spruce (Picea abies)

SILVA FENNICA. Tracheid Cross-sectional Dimensions in Scots Pine (Pinus sylvestris) Distributions and Comparison with Norway Spruce (Picea abies) SILVA FENNICA Silva Fennica 43(4) research articles www.metla.fi/silvafennica ISSN 0037-5330 The Finnish Society of Forest Science The Finnish Forest Research Institute Tracheid Cross-sectional Dimensions

More information

The user should already be familiar with operation of the instrument in STEM mode, use of the Microscope Control interface, and TIA.

The user should already be familiar with operation of the instrument in STEM mode, use of the Microscope Control interface, and TIA. FEI Tecnai F20 S/TEM: EDS system operation Nicholas G. Rudawski ngr@ufl.edu (805) 252-4916 (352) 392-3077 Last updated: 01/22/18 The user should already be familiar with operation of the instrument in

More information

Abstract. 1 Introduction

Abstract. 1 Introduction Development of a system for computer-aided design of ship hulls M. Ventura*, C.Rodrigues\ C. Guedes Scares* "Unitfor Marine Technology and Engineering, Institute Superior Tecnico, Universidade Tecnica

More information

Recovery and Characterization of Non-Planar Resistor Networks

Recovery and Characterization of Non-Planar Resistor Networks Recovery and Characterization of Non-Planar Resistor Networks Julie Rowlett August 14, 1998 1 Introduction In this paper we consider non-planar conductor networks. A conductor is a two-sided object which

More information

SAR AUTOFOCUS AND PHASE CORRECTION TECHNIQUES

SAR AUTOFOCUS AND PHASE CORRECTION TECHNIQUES SAR AUTOFOCUS AND PHASE CORRECTION TECHNIQUES Chris Oliver, CBE, NASoftware Ltd 28th January 2007 Introduction Both satellite and airborne SAR data is subject to a number of perturbations which stem from

More information

IMPACT OF TEXTILE MATERIALS' PARAMETERS ON BEHAVIOUR/TENSION LOADS OF VIRTUAL GARMENTS USING SITTING POSTURE BODY MODELS

IMPACT OF TEXTILE MATERIALS' PARAMETERS ON BEHAVIOUR/TENSION LOADS OF VIRTUAL GARMENTS USING SITTING POSTURE BODY MODELS IMPACT OF TEXTILE MATERIALS' PARAMETERS ON BEHAVIOUR/TENSION LOADS OF VIRTUAL GARMENTS USING SITTING POSTURE BODY MODELS 1 Ta t j a n a KOZ A R, 2 S i m o n a J E V Š N I K, 1 A n d re j a R U D O L F,

More information

HoloMonitor M4. For powerful discoveries in your incubator

HoloMonitor M4. For powerful discoveries in your incubator HoloMonitor M4 For powerful discoveries in your incubator HoloMonitor offers unique imaging capabilities that greatly enhance our understanding of cell behavior, previously unachievable by other technologies

More information