BIO IMAGING. Choose your application of STELLA 2000 STELLA 3200 STELLA BIO Image. light source. light source. light source.
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- Imogen Bridges
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2 Choose your application of BIO IMAGING STELLA 2000 STELLA 3200 STELLA 8300 CCD-camera 2048 x x 1472 pixel pixel 3326 x 2505 pixel light source uv-light table uv-light table uv-light table light source white-light table white-light table white-light table light source variable transilluminator for multifluorescence variable transilluminator for multifluorescence variable transilluminator for multifluorescence light source Epi illumination for multifluorescence Epi illumination for multifluorescence Epi illumination for multifluorescence field of view 12,5 x 12,5 cm 11,5 x 17,5 cm or 15,5 x 23 cm with wide angle lens 20,5 x 27,5 cm cooling Δt = -50 K, Δt = -60 K, below ambient below ambient Δt = -50 K, below ambient program AIDA-bio AIDA-bio AIDA-bio program AIDA-colony AIDA-colony AIDA-colony BIO Image program AIDA-array AIDA-array AIDA-array
3 STELLA 2000 high sensitive modular imaging system APPLICATION Bio/Chemiluminescence Fluorescence, UV to near IR Multichannel fluorescence Western Blots Gel Documentation Proteomics GLP / GMP GENERAL DESCRIPTION FEATURES The STELLA family of imaging systems is based on a modular concept with different high performance modules: several cooled high performance CCD cameras combined with lens optimized for different applications and a variety of modules for illumination ranging from UV to near IR. This guarantees an optimal price to performance ratio for any given application plus the ability to upgrade the system later for additional future applications. STELLA 2000 uses a high performance, cooled, full-frame CCD camera with microlenses for optimal quantum efficiency. 4.0 Megapixel resolution, state of the art CCD architecture and four stage Peltier cooling (abs. -20 C) guarantee high sensitivity and high resolution for chemiluminescent western blots and many other applications. modular / upgradable imaging system manual focus and aperture white light table for protein gels or film digitalisation UV-table for gel documentation variable illumination unit, visible to near IR manual 2 position filter changer AIDA software with many modules incl. GMP / GLP Adding raytest AIDA software for data analysis leads to a high performance chemiluminescence and fluorescence imaging system at affordable price. Optional software modules for GLP / GMP and 21 CFR part 11 allow the operation of STELLA in regulated environment. UV-light table white light table excitation and emission filters mechanical integration of highperformance lenses 154
4 White light image of a coomassie standard SDS-PAGE gel chemiluminescence image of a western blot TECHNICAL DATA ORDERING INFORMATION STELLA 2000 sensor: number of pixels: pixel size: cooled full-frame CCD with on-chip microlenses low dark current 100 % fill factor 2048 x 2048 / 4.0 Mpixel 5.4 x 5.4 μm (unbinned) 10.8 x 10.8 μm (@ 1MP) cooling: 4 stage Peltier cooling (-20 C) digitalisation: 16 bit binning: 2 x 2, 3 x 3, 4 x 4,..., 16 x 16 peak quantum efficiency: 62 % interface: field of view: excitation sources: emission filters: control software: data analysis: USB 2.0 high-speed max x 12.5 cm - UV table (312 nm) - white light table - variable transilluminator with 7 position emission filter wheel - EPI light illuminator manual 2 position filter changer 7 position emision filter wheel easy to operate method-based (optimized methods included) AIDA (network or standalone) state of the art data analysis optimized modules for TLC analysis gels and blots multilabelling whole body autoradiography GLP / GMP, 21 CFR part STELLA 2000 with bright light performance lens Computer controlled focus and aperture white light table for STELLA UV-table 312 nm, 250 x 230 mm variable transilluminator position emission filter wheel xx EPI light illuminator manual filter changer option PC + Windows program installation training, one day huge variety of excitation and emission filters available on request. Please contact your local raytest partner for further information. BIO Image
5 STELLA 3200 ultra high sensitive modular imaging system APPLICATION Bio/Chemiluminescence Fluorescence, UV to near IR Multi-Fluorescence Western Blots Gel Documentation Proteomics GLP/GMP GENERAL DESCRIPTION FEATURES The STELLA family of imaging systems is based on a modular concept with different high performance modules: several cooled high performance CCD cameras combined with lens optimized for different applications and a variety of modules for illumination ranging from UV to near IR. This guarantees an optimal price to performance ratio for any given application plus the ability to upgrade the system later for additional future applications. STELLA 3200 uses a high performance, cooled, full-frame CCD camera with microlenses for optimal quantum efficiency. 3.2 Megapixel resolution, state of the art CCD architecture and four stage Peltier cooling (abs. -35 C) guarantee ultra high sensitivity and high resolution for chemiluminescent western blots and many other applications. modular / upgradable imaging system motorized focus and aperture white light table for protein gels or film digitalisation UV-table for gel documentation variable illumination unit, visible to near IR motorized 7 position filter wheel for fluorescence AIDA software with many modules incl. GMP / GLP Adding raytest AIDA software for data analysis leads to a high performance chemiluminescence and fluorescence imaging system at affordable price. Optional software modules for GLP / GMP and 21 CFR part 11 allow the operation of STELLA in regulated environment. UV-light table white light table excitation and emission filters mechanical integration of highperformance lenses 156
6 white light image of a coomassie stained SDS-PAGE gel Chemiluminescence image of a western blot TECHNICAL DATA ORDERING INFORMATION STELLA 3200 sensor: number of pixels: pixel size: cooled full-frame CCD with on-chip microlenses low dark current 100 % fill factor 2184 x 1472 / 3.2 Mpixel 6.8 x 6.8 μm (unbinned) 13.6 x 13.6 μm (@ 1MP) cooling: 4 stage Peltier cooling (-35 C) digitalisation: 16 bit binning: 2 x 2, 3 x 3, 4 x 4,..., 16 x 16 peak quantum efficiency: 90 % interface: Ethernet field of view: max x 17.5 cm (optional wide angle lens: FOV max. 15 x 23 cm) excitation sources: - UV table (312 nm) - white-light table - variable transilluminator - with 7 position emission filter wheel - EPI light illuminato emission filters: 7 position filter wheel control software: easy to operate method based (optimized methods included) data analysis: AIDA (network or standalone) state of the art data analysis optimized modules for TLC analysis gels and blots multilabelling whole body autoradiography GLP / GMP, 21 CFR part STELLA 3200 without lens STELLA without automatic focus / aperture bright light performance lens bright light, wide angle lens white light table for STELLA UV-table 312 nm, 250 x 230 mm variable transilluminator position emission filter wheel manual filter changer 48006xx EPI light illuminator option PC + Windows program installation training, one day huge variety of excitation and emission filters available on request. Please contact your local raytest partner for further information. BIO Image
7 STELLA 8300 high sensitive, cooled CCD camera APPLICATION Bio / Chemiluminescence Proteomics Fluorescence, UV to near IR Multichannel fluorescence Western Blots Gel Documentation GLP / GMP GENERAL DESCRIPTION FEATURES The STELLA family of imaging systems is based on a modular concept with different high performance modules: several cooled high performance CCD cameras combined with lens optimized for different applications and a variety of modules for illumination ranging from UV to near IR. This guarantees an optimal price to performance ratio for any given application plus the ability to upgrade the system later for additional future applications. STELLA 8300 uses a high performance, cooled, full-frame CCD camera with microlenses for optimal quantum efficiency. 8.3 Megapixel resolution, state of the art CCD architecture and four stage Peltier cooling (abs. -20 C) guarantee high sensitivity and very high resolution for proteomics, chemiluminescent western blots and many other applications. modular / upgradable imaging system motorized focus and aperture white light table for protein gels or film digitalisation UV-table for gel documentation variable illumination unit, visible to near IR motorized 7 position filter wheel for fluorescence AIDA software with many modules incl. GMP / GLP Adding raytest AIDA software for data analysis leads to a high performance chemiluminescence and fluorescence imaging system at affordable price. Optional software modules for GLP / GMP and 21 CFR part 11 allow the operation of STELLA in regulated environment. STELLA on variable transilluminator 158
8 Chemiluminescence image of a 2D western blot multifluorecence image of a 2D SDS-PAGE TECHNICAL DATA ORDERING INFORMATION STELLA 8300 sensor: number of pixels: pixel size: cooled full-frame CCD with on-chip microlenses low dark current 100 % fill factor 3326 x 2504 / 8.3 Mpixel 5.4 x 5.4 μm (unbinned) 10.8 x 10.8 μm (@ 2MP) cooling: 4 stage Peltier cooling (-20 C) digitalisation: 16 bit binning: 2 x 2, 3 x 3, 4 x 4,..., 16 x 16 peak quantum efficiency: 62 % interface: field of view: excitation sources: emission filters: control software: data analysis: USB 2.0 high speed max. 21 x 27 cm - UV table (312 nm) - white light table - variable transilluminator - with 7 position emission filter wheel - EPI light illuminator 7 position filter wheel easy to operate method based (optimized methods included) AIDA (network or standalone) state of the art data analysis optimized modules for TLC analysis gels and blots multilabelling whole body autoradiography STELLA 8300 without lens STELLA without auto focus / aperture bright light, wide angle lens white light table for STELLA UV-table 312 nm, 250 x 230 mm variable transilluminator position emission filter wheel manual filter changer xx EPI light illuminator option PC + Windows program installation training, one day huge variety of excitation and emission filters available on request. Please contact your local raytest partner for further information. BIO Image
9 AIDA-bio analysis program for biological applications APPLICATION contrast optimizing image printing 1-dimensional densitometry (profile analysis) fragment length determination molecular weight determination 2-dimensional densitometry (region of interest analysis) GENERAL DESCRIPTION FEATURES AIDA is designed for the fast and reliable acquisition of quantitative and qualitative data of all kinds of biological samples. AIDA can run under Windows 7 (32- and 64-bit) and Windows 8 ( 32- and 64-bit). Easy and flexible The modular structure of AIDA allows to perfectly match individual application demands with optimal software modules. This results in enhanced usability as the software is restricted to just the required features. At any time AIDA can be upgraded to changing application demands. easy operation fast, accurate results intuitive menus high flexibility professional performance full documentation standalone or network version upgrade with GLP / GMP / 21 CFR 11 module possible Versatile With AIDA it is possible to analyze and save or convert most of the existing image file formats such as TIFF (16 or 8 bit) BMP or JPEG and special file formats from Fuji scanners and cameras and Gel file format. Independent The origin of the digital data may come from a CCD camera, a flat bed scanner, a fluorescence scanner for micro of macro application and / or an imaging plate scanner. Reliable background correction AIDA allows to adapt the background correction to the individual requirements of a large variety of samples
10 AIDA 1D Densitometry fast and precise quantification with automatic lane, baseline and peak determination or with manual functions for special requirements. The quantitation result table is user configurable. AIDA 2D Densitometry Optimal region choice for every type of sample the 2D module contains an appropriate region tool to determine the quantification area. TECHNICAL DATA ORDERING INFORMATION AIDA-bio evaluation: - Manual or automated - 1D (profile analysis) - 2D (region of interest) background correction: - based on profiles - based on background region - local background - integration limits input data from: - CR 35 Bio - HD-CR 35 Bio - most CCD cameras - most flatbed scanners - Fuji BAS / FLA scanners - GE STORM / TYPHOON AIDA bio-package, 1D,2D, FL AIDA basic software AIDA 1D module quantification AIDA 2D module quantification AIDA fragment length determination AIDA bio-package GLP option installation training, one day output file type: - 16 bit TIFF - 8 bit TIFF - JPG - BMP - Fuji or GE file format determination fragment length: - RF value - molecular weight - fragment length - pi value quantity calibration: - linear regression - linear interpolation - logarithmic regression - logarithmic weighted regression - X vs. log Y regression - Y vs. log X regression running under: - Windows 7 ( 32- and 64-bit ) - Windows 8 ( 32- and 64-bit ) BIO Image
11 AIDA-colony data analysis program for colony counting APPLICATION contrast optimizing image printing colony counting variable search areas GENERAL DESCRIPTION FEATURES AIDA is designed for the fast and reliable acquisition of quantitative and qualitative data of all kinds of biological samples. AIDA can run under Windows 7 ( 32- and 64-bit ) and Windows 8 ( 32- and 64-bit ). Easy and flexible The modular structure of AIDA allows to perfectly match individual application demands with optimal software modules. This results in enhanced usability as the software is restricted to just the required features. At any time AIDA can be upgraded to changing application demands. easy operation fast, accurate results intuitive menus high flexibility professional performance full documentation standalone or network version upgrade with GLP/GMP/21 CFR 11 module possible Spot editing manual spot editing functions seperation of double spots creation of undetected spots or erasure of false spots 162
12 Intersections of search areas Flexible definition of intersections is possible Colony detection Two ways of parameter setting: - automatical setting by choosing one or more typical colonies - manual setting of detection parameters TECHNICAL DATA ORDERING INFORMATION AIDA-colony running under: - Windows 7 (32- and 64-bit ) - Windows 8 (32- and 64-bit ) AIDA basic software AIDA-colony counting module option installation training, one day BIO Image
13 AIDA-array data analysis program for array evaluation APPLICATION contrast optimizing image printing import of GAL files normalization alignment array compare module GENERAL DESCRIPTION FEATURES AIDA is designed for the fast and reliable acquisition of quantitative and qualitative data of all kinds of biological samples. AIDA can run under Windows 7 ( 32- and 64-bit ) and Windows 8 ( 32- and 64-bit ). Easy and flexible The modular structure of AIDA allows to perfectly match individual application demands with optimal software modules. This results in enhanced usability as the software is restricted to just the required features. At any time AIDA can be upgraded to changing application demands. easy operation fast, accurate results intuitive menus high flexibility professional performance full documentation array compare module standalone or network version upgrade with GLP / GMP / 21 CFR 11 Versatile Import of GAL files or manual creation of the template. Powerful AIDA allows normalization and up to 9 different correction functions. Images Window Example 164
14 Images Window Example example of a fluorescent labeld array TECHNICAL DATA ORDERING INFORMATION AIDA-array templates: - Manually created - GAL file import normalization: - reference dots - data range (lowest or highest dots) - arithmetic or geometric mean - variance stabilization AIDA basic software AIDA Microarray option installation training, one day background correction: - 9 different correction functions - optimal background for most arrays output file type: - 16 bit TIFF - 8 bit TIFF - JPG - BMP - Fuji or GE file format array compare: - compare a master array to client arrays BIO Image running under: - Windows 7 ( 32- and 64-bit ) - Windows 8 ( 32- and 64-bit )
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