Redefining Gel and Blot Imaging

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1 Redefining Gel and Blot Imaging

2 PXi AND PXi TOUCH Gel and blot imaging made easy Syngene imaging systems are recognised world-wide as high quality, high performance instruments for the capture and analysis of fluorescence gels, chemiluminescence western blots, protein samples and stain free techniques. AUTOMATIC IMAGING MADE EASY The PXi range PXi is the next generation system that sets a new standard in multi-spectral imaging. Its unique, compact design features the latest advancements in CCD technology along with the ground breaking image capture software, GeneSys. Imaging of fluorescence gels and blots, chemiluminescence Western blots, multiplexed western blots, UV-excited and white light applications has never been easier. Sensitivity Achieve superior sensitivity from large pixel size, high quantum efficiency and low read out noise CCD cameras. All cameras have high quantum efficiency of 73% at 425nm resulting in faster and more sensitive image quality. Decrease your exposure time and increase image quality and sensitivity. Use GeneSys image capture software to detect even the faintest of bands using the auto-expose function. Choice of system Choose between two models - standalone with built-in processor and touch screen monitor or add your own PC - and a range of cameras 4m, 6m and 9m pixels to suit budgetary requirements and applications. The ergonomically designed PXi complements any laboratory with its small footprint and fully automated control and application driven image capture software, GeneSys. The PXi system is easy to install and with plug and play Epi LED modules you can effortlessly extend the range of applications that can be imaged.

3 Cameras 16 bit CCD systems available in either 4m, 6m or 9m pixels providing sample resolution as low as 30µm. Cooled to C and a read out noise of typically 5erms delivers exceptional sensitivity ideal for chemiluminescence and IR fluorescence. Lenses Designed to match camera sensors to offer the best possible performance in image quality. The PXi 4 has a superfast f0.95 lens to increase sensitivity by up to 4 times. Syngene has unique flatfix technology which eliminates saturation from images by using an Auto Aperture feature. This technology also includes a lens distortion correction feature which is automatically utilised to give crystal clear images when required. Internal lighting UV imaging Epi UV at 302nm for stain free imaging techniques. Blue light imaging UltraSlim blue LED transilluminator perfect for safe dyes such as SYBR gold, SYBR green and UltraSafe blue. White light imaging Epi white light helps to position samples and image colorimetric markers on chemiluminescent Western blots to aid in the analysis of molecular weights on detected bands. A white light pad is used for imaging Coomassie blue and Silver stained gels. Multiplexed fluorescence imaging Multi-coloured LEDs with 4 excitation channels Red, Green, Blue and IR. Sample drawer The system s smooth glide drawer has guides to enable accurate positioning of the gel or blot. An UltraSlim blue LED transilluminator easily fits into the drawer to image safe dyes. With a small format system, sample size is very important. The PXi systems range from 15.5x11.5cm (PXi 4) to 13x10cm (PXi 6) to 13x10cm (PXi 9). ADVANCED IMAGING TECHNOLOGY

4 POWERED BY GENESYS Applications at the touch of a button GeneSys control software GeneSys has been designed for productivity; its ease of use requires little or no training to acquire publication-quality images. GeneSys acquisition software is application led. At the heart of GeneSys is its extensive database of dyes and protocols. The user only needs to tell GeneSys which application they wish to use and the software automatically configures the system for that application to produce high quality images every time. The GeneSys home screen gives the user the option to use fully automatic mode (preferred) or manual mode. From this screen you can also change your user preferences and access user configurations. Each user can save and load user configurations which are particularly helpful when carrying out repetitive experiments. Configurations save settings such as lens, lighting, filters, exposure time and all the information on your sample type. Multiplex up to five different fluorophores at a time using GeneSys software and then display multichannel fluorescent images in multicolour overlay or in single colour. GeneSys also lets you overlay molecular weight makers and colorimetric markers on chemiluminescent western blots for simultaneous viewing. Also easily view multiple captures of a chemiluminescent blot taken at differing time points. Images captured with GeneSys software can be found on the view screen in GeneSys. You can also view up to four individual images at a time making it easy to compare data. The Edit screen offers a range of editing tools from annotation, rotation, inversion and 3D view. APPLICATION CONTROL AT THE TOUCH OF A BUTTON

5 Select gel or blot type and format Select the type of dye being used from extensive internal database Capture image A full range of editing tools are available to annotate, manipulate, enhance, save and print the image

6 PXi APPLICATIONS Applications you can image with a PXi Chemiluminescence imaging Automated imaging of chemiluminescent blots has never been easier with GeneSys. Achieve faster exposure times, choose between having a speedy image or opting for a high quality image and achieve a dynamic range that surpasses film to ensure fully quantifiable data. Eliminate the guesswork of single event capture of blot signals with film and use GeneSys software to ensure you never saturate your bands and easily capture visible markers that are merged with your chemiluminescent image for molecular calculation. Multiplex fluorescence detection PXi system offers superior sensitivity capable of capturing the broad dynamic range available with fluorescence ensuring outstanding linearity for accurate quantification. Detecting multiple proteins simultaneously is effortless with the new PXi system and GeneSys. Detect up to five different fluorophores on the same gel or blot. Band intensity values can be normalised to another protein or a loading control eliminating the need to strip and re-probe. Multiplexed imaging has made it easier to identify co-migrating proteins such as phosphorylated isoforms and proteins with a similar molecular weight. Image data from each fluorescent channel can be overlaid or viewed individually to detect overlapping bands. ß-actin and p44 ERKp42 HeLa cell lysate probed with anti-erk1/2 and ß-actin primary antibodies followed by IRDye680 and IRDye800 secondary antibodies. HeLa whole cell lysate probed with anti-actin primary antibody followed with anti-mouse HRP secondary antibody. Captured with PXi system with a dynamic range of 1.2 OD ( µg) RNase A -Casein BSA Proteins were probed with anti-bsa, anti- -Casein and anti-rnase A followed by G-Dye100, G-Dye200 and G-Dye300 secondary antibodies. PXi system is a versatile system capable of imaging a wide range of applications from DNA detection with Ethidium bromide or safe dyes to protein analysis of Coomassie blue stain or silver stained gels and fluorescent stained gels and blots e.g. Q-Dots. DyLight, Alexa Fluor, Cy Dye and LI-COR IR dyes as well as stain free imaging. HeLa whole cell lysate probed with anti-actin primary antibody followed with anti-mouse HRP secondary antibody. Exposed to film with a dynamic range of 0.9 OD ( µg) HUNDREDS OF APPLICATIONS AT YOUR FINGERTIPS

7 GEL AND BLOT ANALYSIS WITH GENETOOLS GeneTools analysis software provides rapid and powerful gel analysis. Automatic detection of lanes and bands, viewing densitometry profiles and its user-friendly functions allow for customisation and adjustments to be made. It has never been easier to analyse multiplexed gels or blots with GeneTools making it possible to view and analyse overlaid channels to automatically detect bands in separate channels at the same time and to also view individual channels. Share and export data easily. Save, open and analyse image files, export data reports directly to Microsoft Excel and Word. 1D gel analysis MW/BP calculation Quantification E-gels Band matching with dendrograms Spot blots Colony counting GeneDirectory (option) for extended band matching, cluster analysis, VNTR analysis, genotyping, RFLP studies, dendrogram generation and bootstrapping

8 PXi features and benefits Features Benefits Advanced automation across a large range of applications Easy to use with minimal training Compact design with small footprint Looks good in any laboratory and takes up very little valuable bench space New generation cameras with higher sensitivity and quantum efficiency for capturing low light level images with ease Effortlessly captures high quality images such as western blots Epi and transmitted lighting options for fluorescence including multiplexing Users can work with a wide range of applications within the laboratory, including stain free imaging Built-in processor and screen or external computer options Allows the user to choose a system to meet their needs Please refer to for all ordering information G.055A.03.13

9 PXi specifications PXi 4 PXi 4 Touch PXi 6 PXi 6 Touch PXi 9 PXi 9 Touch Pixels (millions) A/D 16 bit 16 bit 16 bit Greyscales 65,536 65,536 65,536 Cooling Ultra peltier cooling for extra long exposures - REGULATED Ultra peltier cooling for extra long exposures - REGULATED Ultra peltier cooling for extra long exposures - REGULATED Lens F0.95 F1.4 F1.4 Filter wheel motor driven 7 position Built-in processor / touch screen PXi 4 Touch only PXi 6 Touch only PXi 9 Touch only Illumination White epi overhead Epi UV Epi LED for RGB fluorescence Epi LED IR Visible light pad UltraSlim blue LED transilluminator Dimensions (W x H x D cm) 35 x 60 x x 60 x x 60 x 55 Weight PXi 31kg 31kg 31kg PXi Touch 36kg 36kg 36kg

10 Over 75,000 scientists world-wide in pharmaceutical and biotech companies, as well as academic and government institutions, have chosen Syngene as their expert imaging partner. If you d like to find out why, please contact us or one of our dealers for more information and a demonstration of the revolutionary PXi Syngene Europe and International Headquarters: Beacon House Nuffield Road Cambridge CB4 1TF UK Tel: +44 (0) Fax: +44 (0) sales@syngene.com Syngene USA Headquarters: 5108 Pegasus Court Suite M Frederick MD USA Tel: / Fax: ussales@syngene.com Website: Please refer to for all ordering information G All trademarks acknowledged

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