FluorChem M MultiFluor System

Transcription

1 FluorChem M MultiFluor System Advancing Effortless Multiplex Western Blot Imaging

2 Multiplex Western Analysis FluorChem M Imaging System FluorChem M sets a new standard for quantitative multiplex Western blot and gel imaging. Its unique, compact design incorporates state-of-the-art CCD optical technology with an intuitive touchscreen interface for effortless detection of multicolor fluorescent, chemiluminescent, UV-excited and colorimetric gels and blots. Combining three key elements high performance, unmatched ease of operation and true application flexibility FluorChem M takes the complication out of imaging from start to finish. No darkroom, no training, no computer required. 8 Megapixel Resolution Cooled CCD with automated acquisition optics provide exceptional image quality Touchscreen Operation Acquire, review, save and print images using simple on-screen commands Multiple Detection Wavelengths Motorized 10-position emission filter wheel accommodates a wide range of imaging applications Image Storage and Remote Access Integrated computer and hard drive allow for automated image archiving and remote browser-based access White Light Imaging Provides easy sample positioning and imaging of colorimetric blots and colony plates Networking and Printing Network and USB ports for both image retrieval and printing Multiplex Fluorescent Imaging Multicolor LEDs with 3 excitation channels optimized for quantitative Western blot imaging UV Imaging Pull out UV transilluminator for easy loading and imaging of DNA and protein gels Multiplex Westerns Chemiluminescent Westerns UV Fluorescent DNA and Protein Gels Colorimetric Protein Gels

3 Clearly Quantitative Multiplex Fluorescent Detection FluorChem M provides a complete, quantitative solution for Western blot detection and analysis. Direct multicolor fluorescent detection is a stable, non-enzymatic approach that offers greater linearity and dynamic range resulting in superior band clarity and proportional quantitative accuracy Band Intensity ng Transferrin Serial dilutions of Transferrin from 1.0 µg to 0.5 pg were detected by Western blot using MultiFluor Green secondary antibody. The quantitative linear range was above 3 orders of magnitude with a 0.5 pg limit of detection. Serial dilutions of Transferrin were detected by Western blot using MultiFluor Green secondary antibody. The dynamic range of detection was approximately 5 logs. Identify Co-Migrating Proteins Proteins, such as native and phosphorylated isoforms, that overlap due to similar migrating patterns can be probed on the same blot. Image data from each fluorescent channel can be overlaid or viewed independently to readily detect subtle mobility shifts and resolve overlapping bands. Detect Multiple Proteins Simultaneously Save time and precious samples by detecting multiple proteins on the same blot. The need to strip and reprobe is eliminated, resulting in higher data integrity and shorter assay times. Band intensity values can be normalized to another protein or loading control to correct for inaccuracies. ERK 1 Hsp-70 ERK 2 perk 1 perk 2 ERK1/pERK1 ERK2/pERK2 β-actin ERK 1 ERK 2 High Phospho 5 µg 2 µg High/Low 2 µg Low Phospho 2 µg 5 µg HeLa lysates probed with anti- and anti-p primary antibodies followed by MultiFluor Green and MultiFluor Red secondary antibodies. HeLa lysates at 4 µg, 1 µg and 0.25 µg probed with anti-hsp-70, anti-β-actin and anti- primary antibodies followed by MultiFluor Green, MultiFluor Blue, and MultiFluor Red secondary antibodies.

4 Decidedly Uncomplicated DIGITAL DARKROOM Software Are complicated imagers keeping you in the darkroom? FluorChem M s integrated computer and intuitive touchscreen interface make imaging easy. Step 1: Choose Your Application Select from a library of optimized protocols. Step 2: Capture Your Image One-touch acquisition includes instant post-capture image optimization. Step 3: Review Your Results Images are automatically saved and can be immediately printed or copied. Multi-Image Overlay FluorChem M s image overlay capabilities let you easily review and compare data. Display multichannel fluorescent images in multicolor overlay, single color or grayscale. Overlay molecular weight markers on Western blots for simultaneous viewing. Monitor chemiluminescent exposures taken at multiple time points.

5 Breaking Film Boundaries Chemiluminescent Imaging Escape the darkroom and experience how effortless imaging can be on FluorChem M. With faster imaging speeds and a linear range that surpasses film, crucial data are not lost due to under or overexposure. Load less protein, use less antibody, and never miss a faint band again. Anti-ERK antibody, FluorChem M, 5 second exposure FluorChem M vs. Film Anti-ERK antibody, film, 5 second exposure Anti-ERK antibody, FluorChem M, 1 minute exposure Anti-ERK antibody, film, 1 minute exposure Automated Digital Darkroom Take the guesswork out of imaging. FluorChem M s acquisition process automatically determines the optimal exposure and accurately captures the blot s entire signal range. Comparative viewing of virtual exposures eliminates the hassle and expense of taking multiple film exposures. Remote Monitoring and Image Access Monitor acquisition progress and access images on FluorChem M from any networked computer or Wi-Fi enabled device. Our unique browser-based remote interface simplifies image retrieval and is ideal for multi-lab environments.

6 Specifications and Ordering Information FluorChem M Specifications Hardware Detector Optics Light Sources Emission Filters Cabinet Computer Ports Accessories Performance Field of View Dynamic Range Specifications Data Format Size Weight Power Certifications 8 megapixel scientific-grade CCD, 16-bit A/D, thermo-electrically cooled to 25 C absolute and regulated 50-mm, f/1.4 motorized fixed lens RGB fluorescent epi-excitation: 632 nm red, 534 nm green and 475 nm blue LEDs 302 nm UV transilluminator (365 nm optional) Epi-white lights Trans-white light via white light conversion screen 10-position motorized filter wheel 710/40 nm filter 607/36 nm filter 593/40 nm filter 537/26 nm filter Light-tight darkroom UV safety switch Integrated computer with 10.4-inch LCD touchscreen, 250 GB hard drive 3 USB, 1 network Fluorescence imaging screen Chemiluminescence imaging screen UV gel imaging screen White light conversion screen 16 cm x 12 cm maximum Up to 65,536 grayscale 16-bit and 8-bit PNG file output 62 cm D x 62 cm H x 32 cm W (24.5 in x 24.5 in x 12.6 in) 34 kg (75 lb) V AC, 345 W CE System Ordering Information Part # Description FluorChem M System Includes an 8 megapixel camera with a 16-bit A/D, 50-mm, f/1.4 motorized fixed lens; 710, 607, 593, and 537 nm filters; 302 nm UV transilluminator; epi white lights; RGB epi-excitation at 632, 534, and 475 nm; white light conversion screen and fluorescence, chemiluminescence and UV gel imaging screens; integrated computer with LCD touchscreen; FluorChem M Digital Darkroom software and stand-alone AlphaView analysis software Multiplex Application Versatility FluorChem M is compatible with a wide variety of fluorescent labels and stains, allowing existing protocols to be readily adapted. Common Compatible Fluorescent Labels and Stains Fluorescent Labels for Multiplex Western Blotting Cy2, Cy3, Cy5 DyLight 488, 549, 633, 649 Alexa Fluor 488, 546, 555, 633, 647 Fluorescein, FITC Protein Stains Silver Stain Coomassie Blue SYPRO Ruby, SYPRO Red, SYPRO Orange Nucleic Acid Stains Ethidium Bromide SYBR Green, SYBR Gold, SYBR Safe 3040 Oakmead Village Drive Santa Clara, CA Toll-free: (888) Tel: (408) Fax: (408) orders@proteinsimple.com proteinsimple.com 2011 ProteinSimple. ProteinSimple and the ProteinSimple logo are trademarks and/or registered trademarks of ProteinSimple. Rev B.