Supporting Information

Transcription

1 Supporting Information Carbon Nanotubes as a Protein Toxin Transporter for Selective HER2-Positive Breast Cancer Cell Destruction Xuexiang Weng, a,c Meiyan Wang, a Jing Ge, b Shaoning Yu, a Baohong Liu, a Jiang Zhong b and Jilie Kong* a a Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai, , P. R. China b Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai, , P. R. China c College of Chemistry and Life Science, Zhejiang Normal University, Jinhua, P. R. China jlkong@fudan.edu.cn s1

2 A B C File: File: L File: L D E File: m File: L Figure S1: The original cell cytometry data of cells death corresponding to the bars of Figure 4e in the manuscript, respectively. (A)untreated cells, and cells incubated with (B) MWNT alone, (C) RTA alone, (D) MWNT-RTA conjugates and (E) denatured RTA. All the cells are plated at a cell density of cells/well and returned to a CO 2 incubator at 37 C and 5% CO 2 for 22 h and stained by PI (excitation λ = 488 nm, emission detected at λ = 620 nm). s2

3 A B C File: File: File: m D E File: l8.001 File: Figure S2: The original cell cytometry data of cell death vs.concentrations of RTA corresponding to Figure 5a in the manuscript. (A) 0.05 µm RTA, (B) 0.1 µm RTA, (C) 0.2 µm RTA, (D) 0.6 µm RTA and (E) 1.0 µm RTA. s3

4 A B C File: 0.25m.001 File: 0.5m.002 File: 15m D E F File: 2m.003 File: 4m.004 File: 0.25r G H I J File: 0.5r.006 File: 15r.006 File: 2r.007 File: 4r s4

5 K L M File: r.004 File: File: N O File: File: Figure S3: Cell cytometry data of cell death vs. different volumes of MWNT (A-E), RTA (F-J) and MWNT-RTA conjugates (K-O) corresponding to Figure 5b (black, red and green line, respectively) in the manuscript. (A,F,K) 37.5 µl, (B,G,L)75 µl,(c,h,m) 150 µl, (D,I,N) 300 µl and (E,J,O) 600 µl. s5

6 Control, 2 h MWNT alone, 2 h RTA alone, 2 h File: 15m File: File: MWNT-RTA, 2 h Control, 5 h MWNT alone, 5 h File: File: File: L RTA alone, 5h MWNT-RTA, 5 h File: File: 20mr s6

7 Control, 10 h MWNT alone, 10 h RTA alone, 10 h File: File: File: MWNT-RTA, 10 h Control, 15 h MWNT alone, 15 h File: File: L3.003 File: RTA alone, 15 h MWNT-RTA, 15 h File: L2.003 File: L s7

8 Control, 20 h MWNT alone, 20 h RTA alone, 20 h File: File: 15r File: 15kong MWNT-RTA, 20 h Control, 25 h MWNT alone, 25 h File: File: File: L RTA alone, 25 h MWNT-RTA, 25 h File: L File: L Figure S4. Flow cytometry data of cell death vs time of incubation in solutions with MWNT alone, RTA alone, MWNT RTA conjugates and for cells without any treatment (labelled as control ). The data presented herein are corresponding to the data showed in Figure 6 in the manuscript. s8

9 Cos 7, untreated Cos 7, MWNT Cos 7, RTA File: c2.012 File: c1.011 File: c Cos 7, MWNT-RTA Hela, untreated Hela, MWNT File: File: HeLa File: H Hela, RTA File: File: HeLa Hela, MWNT-RTA s9

10 HL7702, untreated HL7702, MWNT HL7702, RTA File: Hl1.016 File: 15m.004 File: Hl HL7702, MWNT- RTA MCF7, untreated MCF7, MWNT File: HL2.003 File: 15kong.003 File: MCF7, RTA File: M File: MCF7, MWNT-RTA Figure S5. Flow cytometry data of cell death of different cell lines for untreated cells and cells incubated in MWNT alone, RTA alone, MWNT RTA conjugates. The data presented herein are corresponding to the data showed in Figure 7 in the manuscript. s10

11 CHO, 1 CHO, 2 CHO, 3 File: L File: L File: cell CHO, 4 CHO, 5 CHO, 6 File: L File: File: CHO, 7 CHO, 8 File: File: s11

12 MDA-MB-453, 1 MDA-MB-453, 2 MDA-MB-453, 3 File: File: File: cell MDA-MB-453, 4 MDA-MB-453, 5 MDA-MB-453, 6 File: File: File: MDA-MB-453, 7 File: File: MDA-MB-453, Figure S6. Flow cytometry data of cell death for normal cell CHO and cancer cell MDA-MB-453 caused by exposure of the cells to various treatments: (1) Untreated cells and cells incubated with (2) MWNT alone, (3) RTA alone, (4) HER2 alone, (5) RTA-HER2 alone, (6) MWNT-HER2 conjugates, (7) MWNT-RTA conjugates and (8) MWNT-RTA-HER2. The data presented herein are corresponding to the data showed in Figure 8 in the manuscript. s12

13 d CP 200 nm e PM CP 2 um s13

14 f CP 200 nm g CP PM 1 um s14

15 h ER CP PM 1 um Figure S7. Ultrathin transverse section of HeLa cells treated without and with MWNT-RTA conjugates for 22h. (d) Subsequent magnifications of white boxed area in figure 3c. (e) MWNTs translocated across the plasma membrane barrier into the neighbouring cytoplasm, (f) A magnification of white boxed area in figure 3e. (g) MWNT bundles located within cytoplasm, (h) MWNT bundles localized nearby endoplasmic reticulum. [MWNTs] = mg ml 1, [RTA] = 0.2 µm. Labels: PM (plasma membrane), CP (cytoplasm), NM (nuclear membrane), N (nucleus), GC(Golgi complex) and ER(endoplasmic reticulum). s15