Simplified Instructions: IXMicro High Throughput Microscope S1225
|
|
- Patience Francis
- 5 years ago
- Views:
Transcription
1 Simplified Instructions: IXMicro High Throughput Microscope S1225 This is an Overview Only intended to refresh specific items learned in the Hands-On Training Use only in conjunction with your own much more detailed notes taken during your training
2 1, Reserve Time on Scheduler 2, Contact Fred if You Wish to Use Something Other Than Configuration B
3 3, Power-Up Turn on Lamp ( I Position)
4 3, Power-Up Turn on IXMicro ( I Position)
5 3, Power-Up Turn on Filter Wheel ( I Position)
6 3, Power-Up Turn on Climate Control ( I Position)
7 3, Power-Up Set Climate Control (right: cell chamber) (left: compound plates)
8 3, Power-Up Turn on Computer ( I Position)
9 4, Access Software Use Windows Log-In/Password provided to you at training
10 4, Access Software Open B Config
11 4, Access Software If not using Config B, open other Configs only after arranging different Config set-up with Fred
12 5, Software Opening Click Yes (relevant only for fluidics)
13 5, Software Opening Server (MDCStore01) Log-In/Password provided to you at training
14 5, Software Opening Highlight User
15 6, Set Instrument Controls Open Screening: Plate Acquisition Setup
16 6, Set Instrument Controls Step Through Menus for Plate Acquisition Setup
17 6, Set Instrument Controls Note: Once Set-Up, simply load existing settings file and jump to Acquire Plate plate after...
18 6, Set Instrument Controls Enter Your Name under Experiment Set (the Set is your folder) Enter Experiment Base Name (i.e. the name of specific study)
19 6, Set Instrument Controls If New Set-Up, Step Through: A, objective/camera settings
20 6, Set Instrument Controls B, Plate Type Highlight only from pull-down. Change nothing else. DO NOT MODIFY ANYTHING HERE EVER
21 6, Set Instrument Controls C, Wells in Plate to image Well at Wells to read
22 6, Set Instrument Controls D, Number and location of fields/well (Adaptive continues until >specified number of cells collected/well)
23 6, Set Instrument Controls E, Time points (for time lapse studies only)
24 6, Set Instrument Controls F, Fluidics (for adding compounds before run only)
25 6, Set Instrument Controls G, General Acquisition Controls Number of wavelengths collected/field Type of Autofocus (laser preferred) Flat-field shading corrections (Yes/No)
26 6, Set Instrument Controls H, Autofocus Locations Plate and Well Bottom recommended First well acquired recommended All sites recommended
27 6, Set Instrument Controls I, Laser Settings Best to Let Fred Work out for your settings Always activate Short Working Distance Always activate Bottom of Well settings
28 7, Set Collection Parameters Set initial collection parameters for each λ Select fluorophore from pull-down, see chart Select Exposure Time (ms) Select Offset initially as 0
29 7, Set Collection Parameters Open Screening: Plate Acquisition and Control Opens Box to Examine Collection Parameters
30 7, Set Collection Parameters Click Find Sample (uses initial settings) Activate Show Live Set Step Size (1 recommended) Count Steps Up or Down for best focus
31 7, Set Collection Parameters Modify collection parameters for each λ Modify Exposure Time (ms) for intensity Modify Offset for best focus
32 8, Finish Collection Set-Up (Most Users will do nothing more) Add in User-written macros (Journals) to modify run in specific ways (knowledgeable Users only)
33 8, Finish Collection Set-Up (Most Users will do nothing more) Activate Display...acquisition if wish to see Click Display Image and size/locate display for manual configuration of display
34 8, Finish Collection Set-Up (Most Users will do nothing more) Starts specified analyses while running (must have analysis parameters set & saved)
35 8, Finish Collection Set-Up (Most Users will do nothing more) Starts specified analyses while running If running on Offline Computers, their control must be activated
36 9, Start Run Acquire Plate under Summary Or (next page)
37 9, Start Run Acquire Plate under Plate Acquisition and Control
38 10, View Data Post-Run Open Screening: Review Plate Data
39 10, View Data Post-Run Select Plate to Review
40 10, View Data Post-Run Select Plate to Review Scroll to your folder Click Date collected Click Experiment Name, Open with Select (can also double-click)
41 10, View Data Post-Run Select Images to Review Check to open thumbnails for fluorophores you wish to view Montage : number of thumbnails to preview starting from highlighted well
42 10, View Data Post-Run Select Images to Review Click on specific thumbnail to open all fluorophore channels for that image Drag arrow to scale image for best viewing
43 11, Image Analysis Select Analysis protocol from pull-down
44 11, Image Analysis Click Configure Settings to open parameter interface for that protocol
45 11, Image Analysis Enter Object classification parameters almost always starts with setting most distinguishable objects ( Nuclei ) Click Preview
46 11, Image Analysis Objects Identified according to parameters are highlighted
47 11, Image Analysis Click indicated button to toggle between actual image and objects classified Modify Object classification parameters and repeat until objects best identified
48 11, Image Analysis Run Analyses for one of -all sites -selected wells highlighted in interface -single site
Simplified Instructions: Olympus Widefield Microscope S1230
Contents General Microscope Operation Simple Image Capture Multi-Wavelength Capture Z-Series Timelapse Combining Capture Modes Synopsis of Other Functions Pages 2-23 24-40 41-47 48-56 57-59 60-68 69-83
More informationSimplified Instructions: Zeiss Brightfield Microscope S1000
Contents General Microscope Set-Up Adjust Illumination Focus Condenser Open Software Image Capture Settings Shading & Color Corrections Image Capture & Viewing Scaling and Measurements Synopsis of Other
More informationCell Biology and Bioimaging Core
Cell Biology and Bioimaging Core Leica TCS SP5 Operating Instructions Starting up the instrument 1. First, log in the log book located on the confocal desk. Include your name, your lab s PI, an account
More informationLeica SP8 TCS Users Manual
Version : 07/08/0 Leica SP8 TCS Users Manual Start up:. Turn the PC Microscope, Scanner Power, Laser Power, and the Laser Emission key to on (bottom right of desk).. Turn on the fluorescent lamp (top left
More informationZeiss LSM 780 Protocol
Zeiss LSM 780 Protocol 1) System Startup F Please note the sign-up policy. You must inform the facility at least 24 hours beforehand if you can t come; otherwise, you will receive a charge for unused time.
More informationLSM 780 Confocal Microscope Standard Operation Protocol
LSM 780 Confocal Microscope Standard Operation Protocol Basic Operation Turning on the system 1. Sign on log sheet according to Actual start time 2. Check Compressed Air supply for the air table 3. Switch
More informationZeiss LSM 880 Protocol
Zeiss LSM 880 Protocol 1) System Startup Please note put sign-up policy. You must inform the facility at least 24 hours beforehand if you can t come; otherwise, you will receive a charge for unused time.
More informationLSM 710 Confocal Microscope Standard Operation Protocol
LSM 710 Confocal Microscope Standard Operation Protocol Basic Operation Turning on the system 1. Switch on Main power switch 2. Switch on System / PC power button 3. Switch on Components power button 4.
More informationLeica SPEII confocal microscope. Short Manual
Leica SPEII confocal microscope Short Manual Switching ON sequence: 1. Turn on the Workstation under the bench (top, far right). 2. Turn on the Supply Unit - Laser box (big green switch first and then
More informationTRAINING MANUAL. Olympus FV1000
TRAINING MANUAL Olympus FV1000 September 2014 TABLE OF CONTENTS A. Start-Up Procedure... 1 B. Visual Observation under the Microscope... 1 C. Image Acquisition... 4 A brief Overview of the Settings...
More informationScanArray Overview. Principle of Operation. Instrument Components
ScanArray Overview The GSI Lumonics ScanArrayÒ Microarray Analysis System is a scanning laser confocal fluorescence microscope that is used to determine the fluorescence intensity of a two-dimensional
More informationb. Turn the power switch and key to on position for blue laser.
OLYMPUS FLUOVIEW 300 CONFOCAL MICOSCOPE OPERATION PROCEDURE 1. Turn ON microscope in this order: 1) Turn on mercury lamp (Note: once the mercury lamp is turned off, DO NOT turn it back on for at least
More informationZEISS LSM510META confocal manual
ZEISS LSM510META confocal manual Switching on the system 1) Switch on the Remote Control button located on the table to the right of the microscope. This is the main switch for the whole system including
More informationGuide to Confocal 5. Starting session
Guide to Confocal 5 Remember that when booking and before starting session you can check for any problems at https://www.bris.ac.uk/biochemistry/uobonly/cif/index.html Starting session Switch on microscope
More informationLeica SP8 TCS Users Manual
Leica SP8 TCS Users Manual Follow the procedure for start up and log on as posted in the lab. Please log on with your account only and do not share your password with anyone. We track and confirm usage
More informationMetaXpress Software: Cell Scoring Module
MetaXpress Software: Cell Scoring Module Cell Scoring Module Overview The Cell Scoring module can be used to analyze cells imaged in 2 wavelengths W1 should be a stain for all nuclei (e.g. DAPI, Hoechst,
More informationOverview. About other software. Administrator password. 58. UltraVIEW VoX Getting Started Guide
Operation 58. UltraVIEW VoX Getting Started Guide Overview This chapter outlines the basic methods used to operate the UltraVIEW VoX system. About other software Volocity places great demands on the computer
More informationOPT3: Operating Procedure for Horiba Jobin Yvon LabRam Aramis Raman/PL System See LabSpec_6_2 General User Quick Start Guide on the computer desktop
OPT3: Operating Procedure for Horiba Jobin Yvon LabRam Aramis Raman/PL System See LabSpec_6_2 General User Quick Start Guide on the computer desktop 1. Log in usage using the SMIF web site 2. Turn power
More informationStandard Operating Procedure
CENTER FOR NANOSCALE SCIENCE AND ENGINEERING Standard Operating Procedure Microscope Software Brian Wajdyk Page 1 of 6 Important Images are not to be saved to the computer. They will be deleted without
More informationMIF ZEISS LSM510 CONFOCAL USER PROTOCOL
MIF ZEISS LSM510 CONFOCAL USER PROTOCOL START-UP Turn on the Mercury Bulb Power Supply (if needed). Power-on the Control Box. Turn on the computer. Open the LSM 510 software. Choose Scan New Images and
More informationStandard Operating Procedure (SOP) for Shared Equipment: Spinning Disk Confocal Microscope
Standard Operating Procedure (SOP) for Shared Equipment: Spinning Disk Confocal Microscope This document is to be used as a supplementary guide and not as a replacement for formal training. DO NOT operate
More informationZeiss Axiovert 135 Fluorescence Microscope Quick Guide / Operations Manual (v. 1.0 February 09)
University of Chicago Integrated Light Microscopy Core Dr. Vytas Bindokas, Director http://digital.bsd.uchicago.edu By: Christine Labno, Assistant Director Room: AB-129 Phone: 4-9040 Zeiss Axiovert 135
More informationOperating Instructions for Zeiss LSM 510
Operating Instructions for Zeiss LSM 510 Location: GNL 6.312q (BSL3) Questions? Contact: Maxim Ivannikov, maivanni@utmb.edu 1 Attend A Complementary Training Before Using The Microscope All future users
More informationNIS-Elements: Grid to ND Set Up Interface
NIS-Elements: Grid to ND Set Up Interface This document specifies the set up details of the Grid to ND macro, which is included in material # 97157 High Content Acq. Tools. This documentation assumes some
More informationTitle: Nikon A1R Confocal User Manual
Title: Nikon A1R Confocal User Manual Date of first issue: 23/10/2015 Date of review: Version: Admin For assistance or to report an issue Office: CG.07 or CG.05 Email: Igmm-imaginghelpdesk@igmm.ed.ac.uk
More informationLSM 800 Confocal Microscope Standard Operation Protocol
LSM 800 Confocal Microscope Standard Operation Protocol Turning on the system 1. Switch on the Main switch (labeled 1 and 2 ) mounted on the wall. 2. Turn the Laser Key (labeled 3 ) 90 clockwise for power
More informationBioanalytik. detect and identify. Over 25 years experience in Imaging. Imaging Software
detect and identify Over 25 years experience in Imaging Imaging Software indigo A short introduction Type User ID and Password to log in indigo A short introduction After entering a password, you see this
More informationUsing Olympus dotslide for polarising microscopy
Using Olympus dotslide for polarising microscopy Background Because the dotslide system is built using a conventional microscope frame it lends itself to acquiring scans in different imaging modes whose
More informationBX-61: Brightfield Instruction /Continue to scroll for Fluorescent Instuctions
BX-61: Brightfield Instruction /Continue to scroll for Fluorescent Instuctions Starting up: Schematic of Olympus BX-61. 1. Turn on Olympus microscope power box (left of microscope) with toggle switch on
More informationQUICKSTART GUIDE: WIDEFIELD HWF1 Zeiss Cell Observer Live Cell Imaging System (HAMMERSMITH, L BLOCK, ROOM 314) Imperial College London
Imperial College London Facility for Imaging by Light Microscopy QUICKSTART GUIDE: WIDEFIELD HWF1 Zeiss Cell Observer Live Cell Imaging System (HAMMERSMITH, L BLOCK, ROOM 314) Observing Life As It Happens
More informationDocuSign for Sugar 7 v1.0. Overview. Quick Start Guide. Published December 5, 2013
Quick Start Guide DocuSign for Sugar 7 v1.0 Published December 5, 2013 Overview This guide provides information on installing and signing documents with DocuSign for Sugar7. The Release Notes for DocuSign
More informationUsing the Nikon TE2000 Inverted Microscope
Wellcome Trust Centre for Human Genetics Molecular Cytogenetics and Microscopy Core Using the Nikon TE2000 Inverted Microscope Fluorescence image acquisition using Scanalytic s IPLab software and the B&W
More informationConfocal imaging on the Leica TCS SP8. 1) Turn the system on. 2) Use TCS user account. 3) Start LAS X software:
Confocal imaging on the Leica TCS SP8 1) Turn the system on. 2) Use TCS user account. 3) Start LAS X software: 4) Do not touch the microscope while the software is initializing. Choose your options: Turn
More informationNikon Eclipse Ti2-E Widefield/Spinning Disk Confocal Microscope Standard Operation Protocol
Nikon Eclipse Ti-E Widefield/Spinning Disk Confocal Microscope Standard Operation Protocol Please sign on the log sheet before switching on system. Turn on system Turn on A only if confocal mode or laser
More informationZeiss AxioImager.Z2 Brightfield Protocol
Zeiss AxioImager.Z2 Brightfield Protocol 1) System Startup Please note put sign-up policy. You must inform the facility at least 24 hours beforehand if you can t come; otherwise, you will receive a charge
More informationNikon AZ100. Laser Scanning Macro Confocal Microscope. Jordan Briscoe Adam Fries Kyle Marchuk Kaitlin Corbin. May 2017.
Nikon AZ100 Laser Scanning Macro Confocal Microscope Jordan Briscoe Adam Fries Kyle Marchuk Kaitlin Corbin May 2017 Contents 1 Introduction 2 2 Hardware - Startup 2 3 Software/Operation 4 3.1 Multidimensional
More informationOpterra II Multipoint Scanning Confocal Microscope. Innovation with Integrity
Opterra II Multipoint Scanning Confocal Microscope Enabling 4D Live-Cell Fluorescence Imaging through Speed, Sensitivity, Viability and Simplicity Innovation with Integrity Fluorescence Microscopy The
More informationSHORT INSTRUCTIONS FOR OPERATING LSM1/2 (Zeiss LSM510) AT CIAN Version 1.4, September 2014
CIAN LSM1 or LSM2 short instructions, version 1.4, September 2014 page 1 of 6 SHORT INSTRUCTIONS FOR OPERATING LSM1/2 (Zeiss LSM510) AT CIAN Version 1.4, September 2014 Before starting To work with LSM1
More informationQUICKSTART GUIDE: WIDEFIELD WF3 Zeiss Cell Observer Live Cell Imaging System (SAF, ROOM 409) Imperial College London
Imperial College London Facility for Imaging by Light Microscopy QUICKSTART GUIDE: WIDEFIELD WF3 Zeiss Cell Observer Live Cell Imaging System (SAF, ROOM 409) Observing Life As It Happens Startup procedure...
More informationZeiss Axio Imager.A1 manual
Zeiss Axio Imager.A1 manual Power-up protocol 1. Mercury lamp 2. Power strip on shelf 3. Computer The Mercury lamp should always be first-on and last-off. This prevents any electrical surges caused by
More informationCAMWorks How To Create CNC G-Code for CO2 Dragsters
Creating the Left Side Smooth Finish Tool Path. This chapter will focus on the steps for creating the left side smooth finish tool path. The objective of this chapter is to create to an accurate and highly
More informationQuick Guide. LSM 5 MP, LSM 510 and LSM 510 META. Laser Scanning Microscopes. We make it visible. M i c r o s c o p y f r o m C a r l Z e i s s
LSM 5 MP, LSM 510 and LSM 510 META M i c r o s c o p y f r o m C a r l Z e i s s Quick Guide Laser Scanning Microscopes LSM Software ZEN 2007 August 2007 We make it visible. Contents Page Contents... 1
More informationWidefield 1. Switching on
Widefield 1 Switching on 1. Ignite DG5 lamp - must be switched on first (if previous user has switched off, wait 30 min before igniting) 2. Wait 5s and then turn on the main DG5 controller switch. 3. DG5
More informationRETRO 3D MOVIE EFFECT
RETRO 3D MOVIE EFFECT Long before Avatar transported us to the breathtakingly beautiful world of Pandora with its state of the art 3D technology, movie audiences in the 1950 s were wearing cheap cardboard
More informationLSM 510 Training Notes
LSM 510 Training Notes Turning on the system Turn on the arc lamp, found on the bench top left of the microscope. This supplies light for epifluorescence for viewing your samples through the microscope.
More informationZEISS LSM 710 NLO Multiphoton microscope Manual/Quick guide
ZEISS LSM 710 NLO Multiphoton microscope Manual/Quick guide Matyas Molnar, Biovis 2016 Starting the microscpe 1. Check the microscope if everything looks clean and normal. If not, report it in the logbook.
More informationThings to check before start-up.
Byeong Cha Page 1 11/24/2009 Manual for Leica SP2 Confocal Microscope Enter you name, the date, the time, and the account number in the user log book. Things to check before start-up. Make sure that your
More informationThermaViz. Operating Manual. The Innovative Two-Wavelength Imaging Pyrometer
ThermaViz The Innovative Two-Wavelength Imaging Pyrometer Operating Manual The integration of advanced optical diagnostics and intelligent materials processing for temperature measurement and process control.
More informationZeiss 880 Training Notes Zen 2.3
Zeiss 880 Training Notes Zen 2.3 1 Turn on the HXP 120V Lamp 2 Turn on Main Power Switch Turn on the Systems PC Switch Turn on the Components Switch. 3 4 5 Turn on the PC and log into your account. Start
More informationMIF ZEISS VIOLET CONFOCAL ZEN 2009 PROTOCOL
MIF ZEISS VIOLET CONFOCAL ZEN 2009 PROTOCOL START-UP On the Switchbox, turn both black switches to the ON position. Wait for the microscope to boot up completely (watch the screen on the side of the microscope).
More informationQImaging. All rights reserved. QUICK START GUIDE
QUICK START GUIDE The QCapture Suite PLUS installer package is available for download on the QImaging website: http://www.qimaging.com/support/downloads/ Once downloaded, the installer folder will be stored
More informationNikon SIM-E & A1-R System
Nikon SIM-E & A1-R System USER GUIDE LSU Health Sciences Center Shreveport Research Core Facility June 01 2017 Chaowei Shang 1 Table of Content 1. Start Up the System... Page 3 Hardware and microscope
More informationMicroscopy from Carl Zeiss
Microscopy from Carl Zeiss Contents Page Contents... 1 Introduction... 1 Starting the System... 2 Introduction to ZEN Efficient Navigation... 5 Setting up the microscope... 10 Configuring the beam path
More informationZEISS LSM 710 CONFOCAL MICROSCOPE USER MANUAL
ZEISS LSM 710 CONFOCAL MICROSCOPE USER MANUAL START THE SYSTEM... 2 START ZEN SOFTWARE... 3 SET THE TEMPERATURE AND THE CO2 CONTROLLERS... OBSERVATION AT OCULARS... 5 STATIF PRESENTATION... 6 ACQUIRE ONE
More informationPractical work no. 3: Confocal Live Cell Microscopy
Practical work no. 3: Confocal Live Cell Microscopy Course Instructor: Mikko Liljeström (MIU) 1 Background Confocal microscopy: The main idea behind confocality is that it suppresses the signal outside
More informationCELL-IQ IMAGEN USER MANUAL
CELL-IQ IMAGEN USER MANUAL FOR CELL-IQ, FOR YOUR RESEARCH NEEDS 2010 Chip-Man Technologies Ltd. All rights reserved. Protected by copyright law and international treaties. Contents 0 Introduction 2 Cell-IQ
More informationUsing Autofocus in NIS-Elements
Using Autofocus in NIS-Elements Overview This technical note provides an overview of the available autofocus routines in NIS-Elements, and describes the necessary steps for using the autofocus functions.
More informationFaculty Lecture Capture Guide
Faculty Lecture Capture Guide If you have never used Panopto before, follow this first part. Log into your Blackboard Account and open the course you wish to capture: Open your Course Management Control
More informationGotSoccer works well with and recommends using Internet Explorer, Safari and Google Chrome.
Cal North CCSL Member Clubs Internet Browser Notice: GotSoccer works well with and recommends using Internet Explorer, Safari and Google Chrome. Some GotSoccer features will not work with Firefox Using
More informationOlympus xcellence Software - basic user guide
Olympus xcellence Software - basic user guide This is a basic overview of setting up time lapse experiments using Olympus's xcellence software on BIU's IX81 inverted phase contrast system - the software
More informationContents STARTUP MICROSCOPE CONTROLS CAMERA CONTROLS SOFTWARE CONTROLS EXPOSURE AND CONTRAST MONOCHROME IMAGE HANDLING
Operations Guide Contents STARTUP MICROSCOPE CONTROLS CAMERA CONTROLS SOFTWARE CONTROLS EXPOSURE AND CONTRAST MONOCHROME IMAGE HANDLING Nikon Eclipse 90i Operations Guide STARTUP Startup Powering Up Fluorescence
More informationLeica Sp5 II Confocal User Guide
Leica Sp5 II Confocal User Guide Turning on the Confocal System (instructions are posted in the room) 1. Turn on Laser Power Button 2. Turn Key to On position 3. Turn on Scanner Power Button 4. Turn on
More informationIncuCyte ZOOM Fluorescent Processing Overview
IncuCyte ZOOM Fluorescent Processing Overview The IncuCyte ZOOM offers users the ability to acquire HD phase as well as dual wavelength fluorescent images of living cells producing multiplexed data that
More informationRENISHAW INVIA RAMAN SPECTROMETER
STANDARD OPERATING PROCEDURE: RENISHAW INVIA RAMAN SPECTROMETER Purpose of this Instrument: The Renishaw invia Raman Spectrometer is an instrument used to analyze the Raman scattered light from samples
More informationUser Guide for TWAIN / DirectX interface for GRYPHAX USB 3.0 cameras
User Guide for TWAIN / DirectX interface for GRYPHAX USB 3.0 cameras The TWAIN & DirectX driver for PROGRES GRYPHAX USB 3.0 cameras enables user to operate with TWAIN and DirectX supported 3 rd party software
More informationZeiss 780 Training Notes
Zeiss 780 Training Notes Turn on Main Switch, System PC and Components Switches 780 Start up sequence Do you need the argon laser (458, 488, 514 nm lines)? Yes Turn on the laser s main power switch and
More informationTitle: Leica SP5 Confocal User Manual
Title: Leica SP5 Confocal User Manual Date of first issue: 23/10/2015 Date of review: Version: Admin For assistance or to report an issue Office: CG07 or 05 Email: Igmm-imaginghelpdesk@igmm.ed.ac.uk Website:
More informationDSU Spinning Disk Confocal and Slidebook 4.2 Quick Guide Light Microscopy Core Facility University of Chicago Vers 0.
DSU Spinning Disk Confocal and Slidebook 4.2 Quick Guide Light Microscopy Core Facility University of Chicago Vers 0.8, February 2007 By: Vytas Bindokas, Ph.D. Core Director 2 The DSU system is optimized
More informationNasmyth Ultraview Vox User Protocol
Nasmyth Ultraview Vox User Protocol Switch on all wall sockets labelled Nasmyth, switch camera on (power supply located on table behind monitor), switch on laser switch in laser rack, switch computer on
More informationFixed cell DSU Spinning Disk Confocal and Slidebook 5.0 Quick Guide. Light Microscopy Core Facility University of Chicago Vers 1.
Fixed cell DSU Spinning Disk Confocal and Slidebook 5.0 Quick Guide Light Microscopy Core Facility University of Chicago Vers 1.0, February 2009 By: Vytas Bindokas, Ph.D., Core Director The fixed cell
More informationOperation Guide for the Leica SP2 Confocal Microscope Bio-Imaging Facility Hunter College October 2009
Operation Guide for the Leica SP2 Confocal Microscope Bio-Imaging Facility Hunter College October 2009 Introduction of Fluoresence Confocal Microscopy The first confocal microscope was invented by Princeton
More informationOlympus Time-lapse Microscope Basic operation
Olympus Time-lapse Microscope Basic operation To start up the microscope 1. Switch on the Olympus UCB. (label as ) 1 Power Switch 2 2. Switch on the MT10. (label as ) Power Switch Page 1 of 18 3 3. Switch
More informationHoriba LabRAM ARAMIS Raman Spectrometer Revision /28/2016 Page 1 of 11. Horiba Jobin-Yvon LabRAM Aramis - Raman Spectrometer
Page 1 of 11 Horiba Jobin-Yvon LabRAM Aramis - Raman Spectrometer The Aramis Raman system is a software selectable multi-wavelength Raman system with mapping capabilities with a 400mm monochromator and
More informationTraining Guide for Carl Zeiss AxioZoom V16 Stereo Microscope
Training Guide for Carl Zeiss AxioZoom V16 Stereo Microscope ZEN 2012 Optical Imaging & Vital Microscopy Core Baylor College of Medicine (2017) Power ON Routine 1 2 If you require fluorescence imaging,
More informationRICOH Stereo Camera Software R-Stereo-GigE-Calibration
RICOH Stereo Camera Software R-Stereo-GigE-Calibration User's Guide RICOH Industrial Solutions Inc. 1/18 Contents 1. FUNCTION OVERVIEW... 3 1.1 Operating Environment... 3 2. OPERATING PROCEDURES... 4 3.
More informationiq-led Software V2.1
iq-led Software V2.1 User Manual 31. January 2018 Image Engineering GmbH & Co. KG Im Gleisdreieck 5 50169 Kerpen-Horrem Germany T +49 2273 99991-0 F +49 2273 99991-10 www.image-engineering.com CONTENT
More informationImaging Features Available in HTML5. it just makes sense
Imaging Features Available in HTML5 it just makes sense August, 2018 Imaging Features Available in HTML5 As part of the 5.2 SP1 release, the Images functionality is now available in HTML5 and provides
More informationDRCR.net Image Acquisition Protocol
DRCR.net Image Acquisition Protocol Optical Coherence Tomography Angiography (OCT-A) Using: Optovue AngioVue Version 3.0 August 14, 2017 DRCR.net OCT-A Optovue AngioVue Procedure Manual 3.0 8-14-17 Table
More informationGetting Started Guide
SOLIDWORKS Getting Started Guide SOLIDWORKS Electrical FIRST Robotics Edition Alexander Ouellet 1/2/2015 Table of Contents INTRODUCTION... 1 What is SOLIDWORKS Electrical?... Error! Bookmark not defined.
More informationLSM 510 Meta Training Notes
LSM 510 Meta Training Notes Turning on the system Turn on X-Cite power supply. This supplies light for epifluorescence for viewing your samples through the microscope. Turn on the remote control switch.
More informationTechnical Note. How to Use the Image Studio Software Western and MPX Western Analyses. Developed for: Image Studio Software
Technical Note How to Use the Image Studio Software Western and MPX Western Analyses Developed for: Image Studio Software Please refer to your manual to confirm that this protocol is appropriate for the
More informationTeam Captain. Online Team Management Guide. Creating Your Team
Team Captain Online Team Management Guide The Anthem Corporate Run wants to help Team Captains manage their teams better. This Guide is meant to help show you how to use the new system and hopefully answer
More informationSTART-UP PROCEDURE 1 THE MICROSCOPE STAND 3 OBJECTIVES 5 STARTING WITH LAS (SOFTWARE) AND SETTING UP THE MICROSCOPE STAND 7
Leica DMI AF6000LX Table of contents START-UP PROCEDURE 1 THE MICROSCOPE STAND 3 OBJECTIVES 5 STARTING WITH LAS (SOFTWARE) AND SETTING UP THE MICROSCOPE STAND 7 ACQUIRE MODULE 6 SETTING THE LIGHTPATH 6
More informationContents. Introduction
Contents Page Contents... 1 Introduction... 1 Starting the System... 2 Introduction to ZEN Efficient Navigation... 5 Setting up the microscope... 10 Configuring the beam path and lasers... 12 Scanning
More informationEverest System / Slidebook Operating Procedures
Everest System / Slidebook Operating Procedures NOTICE: This guide is meant to supplement training, not replace it. All users must be trained first hand by a core employee. Training of others in your lab
More informationFEI Quanta 200 ESEM Basic instructions
FEI Quanta 200 ESEM Basic instructions Desktop and then start the UI. If the computer has restarted and you need to login, Username: supervisor and Password: supervisor Log-in to the Microscope using the
More informationUSB Line Camera 8M. Coptonix GmbH
USB Line Camera 8M Coptonix GmbH Luxemburger Str. 31 D 13353 Berlin Phone: +49 (0)30 61 74 12 48 Fax: +49 (0)30 61 74 12 47 www.coptonix.com support@coptonix.com 2 The USB Line Camera 8M is an easy to
More informationQuick Guide for Zeiss 710 Laser Scanning Confocal MGH Cancer Center
Quick Guide for Zeiss 710 Laser Scanning Confocal MGH Cancer Center For any questions or concerns, please contact: Linda Nieman lnieman@mgh.harvard.edu Office: (617) 643-9684 Cell: (512) 565-8076 Chenyue
More informationTechnical Note. How to Use the Image Studio Software Small Animal Image Analysis. Developed for: Image Studio Software
Technical Note How to Use the Image Studio Software Small Animal Image Analysis Developed for: Image Studio Software Please refer to your manual to confirm that this protocol is appropriate for the applications
More informationLand Cover Change Analysis An Introduction to Land Cover Change Analysis using the Multispectral Image Data Analysis System (MultiSpec )
Land Cover Change Analysis An Introduction to Land Cover Change Analysis using the Multispectral Image Data Analysis System (MultiSpec ) Level: Grades 9 to 12 Windows version With Teacher Notes Earth Observation
More informationRecording EPR Spectra using ER 4102ST Resonator
Recording EPR Spectra using ER 4102ST Resonator This protocol gives step-by-step instructions for recording an EPR spectrum using the high sensitivity Bruker SHQE cavity (assuming the SHQE is already in
More informationIncuCyte ZOOM Scratch Wound Processing Overview
IncuCyte ZOOM Scratch Wound Processing Overview The IncuCyte ZOOM Scratch Wound assay utilizes the WoundMaker-IncuCyte ZOOM-ImageLock Plate system to analyze both 2D-migration and 3D-invasion in label-free,
More informationExercise 1: The AutoCAD Civil 3D Environment
Exercise 1: The AutoCAD Civil 3D Environment AutoCAD Civil 3D Interface Object Base Layer Object Component Layers 1-1 Introduction to Commercial Site Grading Plans AutoCAD Civil 3D Interface AutoCAD Civil
More informationTraining Guide for Carl Zeiss LSM 7 MP Multiphoton Microscope
Training Guide for Carl Zeiss LSM 7 MP Multiphoton Microscope ZEN 2009 Optical Imaging & Vital Microscopy Core Baylor College of Medicine (2017) Power ON Routine 1 2 Turn Chameleon TiS laser key from Standby
More informationGafChromic QuiCk Phantom with EBT3P/3+P Film and FilmQA Pro for Radiation Therapy Dosimetry Applications
GafChromic QuiCk Phantom with EBT3P/3+P Film and FilmQA Pro for Radiation Therapy Dosimetry Applications I. SCOPE The protocol applies to GafChromic EBT3P and EBT3+P films exposed in GafChromic QuiCk Phantom
More informationProject Description. Multispectral Image Capture System The Sixth Sensor
Project Description Multispectral Image Capture System The Sixth Sensor Jocelyn Ramirez, Javier Hernandez, Yu-Cheol Shin, Jonathan Terry, Chris Inderwiesche Revision History: Intro: 2/25/15-20 Use Cases/User
More informationZeiss LSM 510 Confocor III Training Notes. Center for Cell Analysis & Modeling
Zeiss LSM 510 Confocor III Training Notes Center for Cell Analysis & Modeling Confocor 3 Start Up Go to System Module Turn on Main Switch, System/ PC, and Components Switches Do you need the arc lamp?
More informationLeica SP8 Resonant Confocal. Quick-Start Guide
Leica SP8 Resonant Confocal Quick-Start Guide Contents Start-up Preparing for Imaging Part 1 On the scope Part 2 Software interface Part 3 Heat & CO2 incubation Part 4 Other hardware options Shut-down
More informationProgeny Imaging Veterinary
Progeny Imaging Veterinary User Guide V1.14 and higher 00-02-1605 Rev. K1 ECN: ECO052875 Revision Date: 5/17/2017 Contents 1. About This Manual... 6 How to Use this Guide... 6 Text Conventions... 6 Getting
More information3. are adherent cells (ie. cells in suspension are too far away from the coverslip)
Before you begin, make sure your sample... 1. is seeded on #1.5 coverglass (thickness = 0.17) 2. is an aqueous solution (ie. fixed samples mounted on a slide will not work - not enough difference in refractive
More information