Bioanalytik. detect and identify. Over 25 years experience in Imaging. Imaging Software

Transcription

1 detect and identify Over 25 years experience in Imaging Imaging Software

2 indigo A short introduction Type User ID and Password to log in

3 indigo A short introduction After entering a password, you see this page.

4 indigo A short introduction Five Buttons Acquisition for taking images Analysis Projects to define user rights/sample ids Applications to define the test procedure Contact to download new versions

5 Application - Mode The most recommended mode for biginner is selecting the Single Image Choose Petri Lumi

6 Application - Mode In multiday-mode a sample is measured during a period. All images are under one sample id. 1 2 example 3 J14 J17 J21 J24

7 Application - Mode In multiday-multi -sample-mode more samples are measured during a period. For each sample an area is defined which is automatically cut out and saved as an 1 2 Image. If 5 mice are imaged in one shot, you get 5 single images. example 3

8 Application Filters 1 New filter slider Enter Filter name Central wavelength 4 Half bandwidth 5 Transmission

9 Application Filters 2 New filter wheel Enter Filter name Central wavelength Half bandwidth Transmission 4 5

10 Application Selection Select any operation, move it into selections. 1 Any combination is possible!

11 Application Selection One click shows parameters in the right box 2 Double click opens parameter check box! 1 3

12 Application Sample size Sample size means the field of veiw Sample height means the focus distance from the bottom plate to the target 4

13 Application View Settings Gives you an overview.

14 Projects - Settings Projects to define user rights/sample ids

15 Projects Analyse Settings Presetting for Analysis. If all is set to 0, it is auto-scalling.

16 Projects User rights Select any person. Any combination is possible!

17 Projects View Settings 1 Gives you an overview. 2

18 Acquisition- Select An Existing Project Select a project to start the acquistion

19 Acquisition- Type Sample ID And Generate Sequence Number The date and project name come out automatically Input the sample ID and press the generate key to generate the sequence generate number

20 Acquisition- Check Filters and Teach In For fluorescence Imaging Press the Check key to check the Ex. filters and the Em. filters

21 Acquisition- Check Filters and Teach In For fluorescence Imaging For the project containing the fluorescence imaging, press the Teach in key to record the excitation energy During Teach In process, do not leave targeted objects inside the dark chamber

22 Acquisition- Quick Check And Acquire Press the Quick Check Key to view the focus and position of targeted objects 1.

23 Acquisition- Quick Check And Acquire 2. Press the Quick Check Key to view the focus and position of targeted objects

24 Acquisition- Quick Check And Acquire Press the Acquire key to start the acquisition After Acquisition, the image is saved automatically 1

25 Acquisition- Quick Check And Acquire 2. Press the Acquire key to start the acquisition After Acquisition, the image is saved automatically

26 Analyse Define everything Rulers for contrast Rulers for signal intensities

27 Analyse Define everything Rulers for transparency: One for signal One for photo

28 Analyse tool bar Analyse Powerful tools Analyse tools facilitate the accurate quantitation and comparision of data

29 Analyse tool bar Analyse Powerful tools Even more sophisticated 1.

30 Analyse tool bar Analyse Powerful tools Even more sophisticated 2.

31 Analyse tool bar Analyse Powerful tools Easily print out the 1. report or export to Excel

32 Analyse tool bar Analyse Powerful tools Easily print out the 2. report or export to Excel

33 Analyse ease of analysis Define the image Number of your experiments 1.

34 Analyse ease of analysis Use rulers for contrast 2. and signal intesities

35 Analyse ease of analysis Apply for sample-id, of all images of this experiment are treated in the same way. 3.

36 Analyse ease of analysis 4. Finally go can start a slide show

37 detect and identify Thanks for Your attention Over 25 years experience in Imaging