Enterobacter aerogenes. Colony Morphology. Colony morphology gives important clues as to the identity of their constituent microorganisms.

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1 Enterobacter aerogenes Colony Morphology Colony morphology gives important clues as to the identity of their constituent microorganisms.

2 The Winner: Most disgusting bacterial colony I have ever seen

3 Comes From a Hot Pot in Yellowstone Park >>>>>

4

5 The coolest colonies I ve ever seen >>>

6 Made from glowing bacteria.

7 How many different types of bacteria are growing on this plate?

8 Colony Morphology Size: Colony shape: Margin (edge): Elevation: Texture: Light Transmission: Color:

9 Colony Size Dependent on the: type of organism the growth medium the number of colonies present on a plate culture medium characteristics. Colony size usually stabilizes after a day or two of incubation.

10 Colony Shape:

11 Fillimentous

12 Rhizoid

13 Margin:

14 Undulate

15 Lobate

16 Curled

17 Filimentous

18 Errose

19 Colony Elevation Colonies can vary in their elevations both between microorganisms and growth conditions, and within individual colonies themselves.

20 Flat vs Raised

21 Convex

22 Pulvinate

23 Umbonate

24 Raised Margins

25 Extreme Example of Crateriform

26

27 Colony Texture Possible textures include: shiny to dull smooth to wrinkled rough granular mucoid A shiny, smooth, and/or mucoid appearance tends to be associated with the presence of capsular material.

28 Rough

29 Dull / Granular

30 Smooth

31 Mucoid

32 Wrinkled (Red colonies only)

33 Colony Light Transmission The light transmission through colonies can range from: complete (transparent) through intermediate (translucent) through completely lacking (opaque)

34

35 Colony Pigmentation Colonies can come in a rainbow of colors.

36 Bacillus subtilis Colony shape and size: irregular, large Margin: undulate (wavy) Elevation: umbonate Color: white, dull Texture: dry (or rough).

37 Enterobacter aerogenes Colony shape and size: round and small Margin: entire Elevation: convex Color: yellow/tan Texture: shiny

38 Klebsiella pneumoniae Colony shape and size: round and large Margin: undulate Elevation: unbonate Color: white translucent Texture: muciod

39 Serratia marcescens Colony shape and size: small and round Margin: entire Elevation: umbonate Color: red and white Texture: Mucoid

40 Bacillus subtilis Colony shape and size: irregular, large Margin: undulate (wavy) Elevation: umbonate Color: white, dull Texture: dry (or rough).

41 Bacillus megaterium Colony shape and size: large and irregular Margin: unbonate Elevation: convex Color: white Texture: dull

42 Enterobacter aerogenes Colony shape and size: small and round Margin: entire Elevation: convex Color: yellow/tan Texture: shiny

43 Enterobacter aerogenes Colony shape and size: small and round Margin: entire Elevation: convex Color: yellow/tan Texture: shiny

44 Escherichia coli Colony shape and size: Round and small Margin: entire Elevation: slightly raised Color: cream Texture: mucoid

45 Lactobacillus acidophilus Colony shape and size: round and punctiform Margin: smooth Elevation: convex Color: white Texture: smooth Rogosa Agar

46 Micrococcus luteus gram positive tetracoccus

47 Micrococcus luteus Colony shape and size: circular, pinhead Margin (edge): entire Elevation: Convex Color: yellow, non-diffusable pigment Texture:

48 Mycobacterium smegmatis Colony shape and size: irregular and small Margin: lobate Elevation: flat Color: orange-yellow, waxy Texture: dry

49 Proteus vulgaris Colony shape and size: Small and round Margin: entire Elevation: convex Color: tan Texture: muciod Note: This plate illustrates the typical "swarming" characteristic of the Proteus genus. Notice how the colony has moved out in

50 Pseudomonas aeruginosa Colony shape and size: oval and medium Margin: wavy Elevation: umbonate Color: diffusible green Texture: mucoid fruity odor

51 Rhodospirillum rubrum Colony shape and size: Pinpoint circular Margin: entire Elevation: convex Color: red pigment Texture: shiny

52 Staphylococcus aureus Colony shape and size: circular, pinhead Margin: entire Elevation: convex Color: golden-brown Texture: smooth

53 Staphylococcus aureus Colony shape and size: circular, pinhead Margin: entire Elevation: convex Color: golden-brown Texture: smooth

54 Staphylococcus epidermidis Colony shape and size: circular, pinhead Margin: entire Elevation: convex Color: agar or whitish Texture: mucoid

55 Vibrio anguillarum

56 Vibrio anguillarum Colony shape and size: large and irregular Margin (edge): entire Elevation: convex Color: Tan Texture: Mucoid

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