Visible & UV/Vis Spectrophotometers

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1 Visible & UV/Vis Spectrophotometers Accurate Precise Affordable

2 S20 Series UV/Vis Spectrophotometers The Unico S20 Series Spectrophotometers are simple, flexible, easytouse and maximize value while providing performance you would expect for a 5nm bandpass instrument. The S20 Series simplicity is reflected in its attractive price without sacrificing accuracy and precision. The value is evident from the performance provided every day, all year. The flat, lowprofile design minimizes bench dead space, allowing room for cuvette racks, notebooks or a laptop computer. To simplify documentation, the S20 Series spectrophotometers can be controlled from UNICO Application Software for Windows through the builtin RS232C port. The standard software package expands the applications to include standard curve, Kinetics (Abs. vs Time) and the advanced software package further adds DNA/Protein analysis and Scanning capabilities. Test data can be exported into Microsoft Excel for further analysis. EasytoUse, EasytoRead Display The twoline, LED display is large enough to read from any angle and distance, so there is no need to search or squint. For UV models (S20UV and S20SUV), a simple push of the button on the display panel allows you to turn on/off either lamp, extending the lifetime. Readily switch modes from Absorbance to % Transmittance, or enter a Concentration or Dilution Factor. No complicated menu to slow down technicians from completing other important tasks. The standard large, flexible sample compartment has a smooth 4cell holder with 4 glass cuvettes and 2 quartz cuvettes (for UV models only) included. A wide variety of accessories are available. Four models to choose from: S20 Visible Spectrophotometer S20S Visible Scanning Spectrophotometer S20UV UV/Vis Spectrophotometer S20SUV UV/Vis Scanning Spectrophotometer Specifications Model Wavelength Range Bandwidth Optical System Wavelength Accuracy Wavelength Repeatability Wavelength Readability Photometric Range Photometric Accuracy Stray Light Stability Standard Cell Holder Sample Compartment Display Output Software Light Source Power Requirements Instrument Dimensions Instrument Weight S20 & S20S (Visible) S20UV & S20SUV (UV/Vis) 325nm 00nm 200nm 00nm 5nm 5nm Single beam, grating system 200 lines/mm Single beam, grating system 200 lines/mm ±nm ±nm nm nm 025%T, 0.2.5A, 0999 C (0999F) 025%T, 0.2.5A, 999C (0999F) 340nm & 400nm & 340nm 4position mm cell changer 4position mm cell changer Accommodates 0mm pathlength cuvette with optional holder Twoline, 9digits Twoline, 9digits RS232C RS232C Optional (S20), Standard (S20S) Optional (S20UV), Standard (S20SUV) Tungsten Halogen Lamp Tungsten Halogen/Deuterium Lamp 5V/230V switchable 5V/230V switchable 470mm(W) x 400mm(D) x 40mm(H) 470mm(W) x 400mm(D) x 40mm(H) 8.5 (W) x 5.7 (D) x 5.5 (H) 8.5 (W) x 5.7 (D) x 5.5 (H) 2kg (26 lbs) 4.5kg (32 lbs) Simple Solutions for your Spectrophotometry Needs ~ ~

3 S20 Series UV/Vis Spectrophotometer Application Software Install and start collecting your data The UNICO Application Software expands your applications, assists in your data documentation and provides complete control of the spectrophotometer from a computer. Applications include:. Abs./ %T/Conc. 2. Standard Curve (Quantitative) 3. Kinetics (Abs. vs Time) 4. Scanning (with advanced software package #S20402) 5. DNA/Protein Abs/%T/Conc Measure absorbance, transmittance or concentration with standard or known factor. Quantitative (Standard Curve) Use up to 8 standards to establish standard curve. Four methods for fitting a curve:. Linear fit 2. Linear fit through zero 3. fit 4. Segmented Wavelength Scanning (advanced software package #S20402). Hi, Low scan speed selectable 2. Automatically record peaks and valleys 3. Rescaling axes, curve Kinetics (Abs vs. Time) The Kinetics mode may be used for time course scanning or reaction rate calculations. Abs. vs. time graphs is displayed on the screen in real time. Wait time, measurement time and time intervals may be entered. DNA/Protein Concentration and DNA purity are calculated: Absorbance ratios 260nm/280nm with optional subtracted absorbance at 320nm. DNA Concentration =62.9 x A x A280 Protein Concentration =552 x A x A280 Other wavelengths and factors may be entered. Application Software Ready to Go ~ 2 ~

4 S20 Series Accessories and Options Test Tube Holder (Item# S20P) Test tube holder kit for 820mm diameter test tubes. Includes universal base, Vtype tube holder. The maximum tube height is 0mm. Long Path Cell Holder (Item# S202P) long path cell holder kit for single cell up to 0mm pathlength. Includes universal base and one holder. Single Cell Holder (Item# S203P) Single square cell holder mm pathlength cuvette. Cylindrical Cell Holder (Item# S204P) Cylindrical cell holder kit for single cell up to 0mm pathlength (20mm dia.). Includes universal base and one holder. WaterJacketed Cell Holder (Item# S205P) WaterJacketed single cell holder kit including universal base and one waterjacketed cell holder for mm square cuvette. It maintains desired temperature by circulating constanttemperature water from water bath (water bath required and not included). Micro Cell Holder (Item# S206P) Measure a sample with volume of 0uL using micro cell holder. The xy adjustable mechanism is used to align cell with optical beam for optimized results. Peltier Unit (Item# S207P) Peltier unit for continuous temperature control from 5 to 40º C. The xy adjustable mechanism is used to align cell with optical beam for micro cell setup. The temperature display resolution is 0.ºC. The unit consists of a controller and a thermoelectrically controlled cell holder. Ambient Sipper Unit(Item# S208P) Sipper system for single cell flow thru. The xy adjustable mechanism is used to align cell with optical beam for micro flow cell setup. The sipper unit consists of a flowthru controller with peristaltic pump and a flowthru front panel (flow cell and tubing not included). Note: Requires flow cell and proper tubing to complete flow thru setup. Peltier/Sipper System (Item# S209P) Peltier/Sipper system for single mm cell flow thru and continuous temperature control from 5 to 40º C. The xy adjustable mechanism is used to align cell with optical beam for micro flow cell setup. The temperature display resolution is 0.ºC. The unit consists of a Peltier/Sipper controller with peristaltic pump and a thermoelectrically temperature controlled cell holder with panel. The unit can be used as flow thru only or temperature control only. Note: Requires flow cell and proper tubing to complete flow thru setup. Reflectance Measurement Attachment (5º incident angle) (Item# S20) The technique of reflectance measurement is used for evaluation of materials relative to a reflectance surface. The minimum sample is (L) 30 x (W) 30 mm. Cuvettes A large variety of optical glass and quartz cuvettes available including standard square glass cells, quartz cells, long path cells, semimicro and micro cells, short path cells, cylindrical cells, flow thru cells. See price list for individual item number listings. S20 Series Accessories to Fit your Needs and Budget ~ 3 ~

5 S200 Series Visible Spectrophotometer Specifications: S200 Series Wavelength Range Bandwidth Optical System Wavelength Accuracy Wavelength Repeatability Photometric Range Photometric Accuracy Stray Light Output Sample Compartment Light Source Power Requirements Instrument Dimensions Instrument Weight 325nm 00nm 5nm Single beam, grating system 200lines/mm ±nm 025%T, 02.0A, 999C (0999F) ±%T <0.5%T at 340nm & 400nm RS232C Accommodate 50mm pathlength cuvette with optional holder Tungsten Halogen Lamp 5V/230V switchable 408mm x 308mm x 80mm 6 (W) x 2 (D) x 7 (H) 6.5kg (5 lbs) UNICO S200 Series is the best value 5nm design. High quality silicon photodiode detector and 200 lines/mm grating assures high performance. Large digital display and builtin filters for easy operation. It allows for Absorbance, Transmittance and Concentration modes, also for automatic zeroing and blanking with a single button. The S200 Series features optional Windows based software for easy data collection and application expansion to standard curve and kinetics. Data can be easily exported to Microsoft Excel for further processing and analysis. A toolfree and alignmentfree lamp change, large sample compartment packaged with a variety of optional accessories, makes the easytouse UNICO S200 ideal for any standard spectrophotometer application, while staying within a conservative budget, CE approved. Model S200 comes standard with test tube holder and single square cuvette holder. The model S20 is packed with 4cell changer, standard. Ordering Information S200 S200E S20 S20E S20040 S0 S200 S2002 S2003 S2004 S2005 S200 S Model S200 Spectrophotometer 5nm bandwidth, RS232C port. Complete with mm single square cuvette holder, universal test tube holder, mm test tube (box of 2), a pair of optical glass cuvettes, dust cover, and operator s manual. Voltage preset at V. Same as S200 but voltage preset at 220V. Model S20 Spectrophotometer 5nm bandwidth, RS232C port. Complete with 4cell holder, a pair of optical glass cuvettes, dust cover, and operator s manual. Voltage preset at V. Same as S20 but voltage preset at 220V. Application WindowRbased software for PC. Programs include Standard Curve, Abs/%T/Conc., Abs. vs Time. Inludes RS232C serial cable (#S200226). Experimental/operators manual for UNICO 0 and 200 series. Experiments include BeerLambert, Absorption spectrum, Ksp of Pbl 2, Chlorophyll, Protein (Biuret test), and more. Includes teacher s guides, illustration, and answers to questions (60 pages) Universal test tube holder with base for 8 to 25mm diameter test tubes including COD. Single cell holder with base for mm square cuvettes. 4position cell holder for mm square cuvettes. Longpath 4position cell holder for up to 50mm rectangular cuvettes. Waterjacketed mm single cell holder with base and panel for temperature control applications (requires water bath). Black block for setting 0%T. Replacement Lamp. Universal test tube holder mm single cell holder 4position mm cell holder Longpath cell holder (Item# S200) (Item# S2002) (item# S2003) (Item# S2004) The Reliable and Expandable Visible Spectrophotometer Waterjacketed cell holder (Item S2005) ~ 4 ~

6 S0 Series Visible Spectrophotometers S0 Series is designed for use with ½ (3mm) round tubes or mm square cuvettes. Tube holders available for COD, 3/4 and tubes. An adapter is supplied for mm square cuvettes. High quality silicon photodiode detector and 200 lines/mm grating assures high performance. Auto zero function, pressbutton blanking and builtin filters for easy operation. Large digital display for easy readout. A toolfree and alignmentfree lamp change reduces the need for instrument service. The Model S0RS features optional Windows based software for easy data collection and application expansion to Standard Curve and Kinetics. Data can be easily exported to Microsoft Excel for further processing and analysis. S0 Model S0 has Absorbance, Transmittance with Analog output. Model S0RS has RS232C output and additional Concentration and Factor modes. Specifications Model Wavelength Range Bandwidth Optical System Wavelength Accuracy Wavelength Repeatability Photometric Rang e Photometric Accuracy Stray Light Output Sample compartment Light Source Power Requirements Instrument Dimensions Instrument Weight S0 335nm 00nm 20nm Single beam, grating system 200lines/mm ±nm 025%T, 02.0A ±2%T <0.5%T at 340nm & 400nm Analog Port Tungsten Halogen Lamp 5V/230V switchable 408mm x 308mm x 80mm 6 (W) x 2 (D) x 7 (H) 6kg (3 lbs) Accommodates inch round tube with optional holder S0RS S0RS 335nm 00nm nm Single beam, grating system 200lines/mm ±nm 025%T, 02.0A, 999C (0999F) ±%T <0.5%T at 340nm & 400nm RS232C Interface Tungsten Halogen Lamp 5V/230V switchable 408mm x 308mm x 80mm 6 (W) x 2 (D) x 7 (H) 6kg (3 lbs) Ordering Information S0 S0E S0RS S0RSE S040 S0 S02 S03 S03 S04 S05 S0505 S052 Model S0 Spectrophotometer 20nm bandwidth, Analog output port. Complete with mm test tube holder, mm test tube (box of 2), square cuvette adapter, dust cover, and operator s manual. Voltage preset at V. Same as S0 but voltage preset at 220V. Model S0RS Spectrophotometer nm bandwidth, RS232C port. Complete with mm test tube holder, mm test tube (box of 2), square cuvette adapter, dust cover, and operator s manual. Voltage preset at V. Same as S0RS but voltage preset at 220V. Application Windows based software for PC. Programs include Standard Curve, Abs/%T/Conc., Abs. vs Time. Inludes RS232C serial cable (#S0226). Experimental/operators manual for UNICO 0 and 200 series. Experiments include BeerLambert, Absorption spectrum, Ksp of Pbl 2, Chlorophyll, Protein (Biuret test), and more. Includes teacher s guides, illustration, and answers to questions (60 pages). Test tube holder for 3mm diameter (mm pathlength) test tube (included with instrument). cuvette adapter for mm square cuvettes (included with instrument). Test tube holder for ¾ inch test tubes. Test tube holder for inch test tubes. COD vial holder. Replacement Tungsten Halogen Lamp. Allen Wrench (for sample holder installation). The Reliable and Simple Viasible Spectrophotometers ~ 5 ~

7 The EasytoUse S00 Spectrophotometer for Educational Labs Model S00 Spectrophotometer Wavelength Range Spctral Slit Width Optical System Wavelength Accuracy Wavelength Repeatability Stray Light Photometric Range Photometric Accuracy Output Light Source Power Requirements Instrument Dimensions Instrument Weight nm 20 nm Single beam, grating 200 line/mm ±3 nm ± nm <2% at 400 nm 00%T, 0.99Abs ± 2% Analog Tungsten Halogen Lamp 6V/W V/220V Switchable 6 (W) x 2 (D) x 8 (H) 406mm x 305mm x 205mm 5 lbs (7kg) Models S00 Spectrophotometer Precisely designed and ruggedly built, this easytouse spectrophotometer is ideal for student use in high schools and colleges. It is suitable for general analysis and experiments such as BeerLambert, Absorption Spectrum, Chlorophyll, protein (biuret test) and more. The builtin secondary filters reduce stray light and increase precision. The studentproof positive safety stopper integrated with the wavelength dial prevents damage to the monochromator and grating. Large digital display makes reading much easier and analog interface allows customers to collect data or print easily with external analog device. The spectrophotometer comes with ½ inch round tube holder, mm square cuvette adapter, box of 2 round tubes, user s manual, and dust cover. It is set for V, but is switchable to 220 V as well. It is fully packed and ready to use. Cells Type Optical Pathlength (mm) Interior width (mm) Window Height (mm) Height (mm) Capacity (ml) Quantity per package Glass Cell Quartz Cell Polystyrene. Cell Round Tube S9030 S90302P 0, lid Cylindrical Cylindrical Flowcell Flowcell Ø22 Ø (chamber) 0 S90304G S90350G S90330G S90325G S9032G S9032G S90327G S G S903080G S90340FG S90344FG S90309Q S9035Q S90358Q S90359Q S9033Q S903Q S9033Q S9032Q S903Q S9034Q S903FQ S90346FQ S90305P Flowcell Ø (chamber) S903FG S90347FQ Cells Round Tube Cylindrical Microcell Flowcell ~ 6 ~

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