Colony Imaging with powerful Analysis Software

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1 TM Colony Imaging with powerful Analysis Software TM Accurate Compact Fast

3 We re not going to interpret your results, but we ll do everything to get you there From image acquisition to data visualisation, straight out of the box. singerinstruments.com

Introduction The world's fastest colony imaging & analysis! What is it? The PhenoBooth is a fast, semi-automated plate reader for imaging and analysis of cell colonies.

4 Introduction The world's fastest colony imaging & analysis! What is it? The PhenoBooth is a fast, semi-automated plate reader for imaging and analysis of cell colonies. Its open-architecture enables fully customisable workflows. Data export and import is possible at any stage, as well as having compatibility for LIMS integration. What does it do? The PhenoBooth takes a high-resolution image of colonies on any number of plates, and then Pheno- Suite s integrated batch analysis workflow takes the user through: Image Pre-processing, Measurement, Normalisation, Analysis and Visualisation, with a single-click. Application specific software packages, such as SGA Analysis or Zone Inhibition are integrated seamlessly. Using advanced PhenoSuite software it can perform time-lapse imaging, generate colony growth curves, and automate a multitude of downstream analysis with minimal user-input.

colour Time-lapse imaging for colony growth Automated normalisation and analysis of SGA data How does it work?

every time. Once you have acquired all of your images, they are seamlessly transferred to our powerful batch analysis software.

5 What can I use it for? Imaging of standard sbs plates and Singer PlusPlates Colony counting Automatic colony feature measurement including: size, density, pixel count, circularity and colour Time-lapse imaging for colony growth Automated normalisation and analysis of SGA data How does it work? The PhenoBooth contains a 13-megapixel camera and top and bottom SpectraStar TM flat-field illumination technology, which produces high-resolution colony images every time. Once you have acquired all of your images, they are seamlessly transferred to our powerful batch analysis software. The powerful PhenoSuite software accurately counts and measures the selected colony features. Image capture can also be scheduled to analyse cell size over a specified period of time and create detailed growth curves of each colony. The PhenoSuite can be customised with different work packages to automate your favourite analysis workflow. PhenoSuite filtering feature. singerinstruments.com

Hardware Features The PhenoBooth is extremely quick and easy to use. Its outstanding image quality makes it an invaluable tool for colony time-lapse imaging.

6 Hardware Features The PhenoBooth is extremely quick and easy to use. Its outstanding image quality makes it an invaluable tool for colony time-lapse imaging. Gudjon Olafsson MRC National Institute for Medical Research Back light Integrated SpectraStar TM back light for high-fidelity, flat-field, transmitted light images. Incubation Compact size to operate within an incubator. Function light Light indicator to signal operation type. Input tray Plate tray designed for manual operation or stacker integration. Top light Integrated SpectraStar TM top light for high-fidelity, flat-field incident light images. USB Connections for computer interface.

Software Features Colony counting Colony sizing Normalisation & scoring for SGA PhenoSuite colony growth analysis feature. Time-lapse imaging Customise software Accurate at 6k Export.

7 Software Features Colony counting Colony sizing Normalisation & scoring for SGA PhenoSuite colony growth analysis feature. Time-lapse imaging Customise software Accurate at 6k Export.csv for single colony picking Colony Colour Software Overview Software Features PhenoSuite software uses Singer s signature easy-touse interface to automatically and accurately measure many colony properties. PhenoSuite is powerful yet flexible, offering a variety of custom-made work packages to automate your entire analysis workflow. Image acquisition at 3300x2244 with a pixel size of 34µm Full control of imaging parameters including (but not limited to): brightness, gain, exposure, saturation, hue, contrast and white balance. Open architecture design. Compatibility for LIMS integration. Colony separation >= 1 pixel Colony gridding. Colony detection for all colonies >= 1x1 pixel Automatic, fully customisable agar and colony detection. Adjustable filters to remove detected objects based on size, shape and colour. Data provided on colonies includes size, density, pixel count, circularity, colour and length of perimeter. Time-lapse/scheduled growth monitoring. Zone inhibition measurement. Accurately analyse colonies at 6k density. Automated normalisation and SGA analysis. Spectrastar top & bottom flat-field illumination One-click colony analysis Automatic data storage & management singerinstruments.com

Technical specifications Standard hardware set-up Model: PhenoBooth product code: PHB-001 Technical Specs: Footprint: Width: 305mm Length: 305mm Height: 316mm Weight: 16kg Power: 100-200 VAC 50-60 Hz

8 Technical specifications Standard hardware set-up Model: PhenoBooth product code: PHB-001 Technical Specs: Footprint: Width: 305mm Length: 305mm Height: 316mm Weight: 16kg Power: VAC Hz Max Power Consumption: 65w Camera specifications 13MP Sony CMOS Chip Auto focus lens Standard Software set-up Model: PhenoSuite product code: PHS-001 Hardware / Software Requirements Intel Core i3 or similar, 2 GB RAM Graphics card with 24 or 32 bit Windows XP, Windows Vista, Windows 7 (32 & 64 bit), Windows 8 (32 & 64 bit) USB 3.0 port Support Product name: PhenoBooth Support Product name: PhenoBooth Support Plus Imaging Speed 720 plates/hour 1 year product code: SPB year product code: SPB year product code: SPB year product code: SPB-130 All information is correct at the time of printing. Some revisions may be made as specifications are improved. THE COMPLETE HIGH-THROUGHPUT SCREENING SOLUTION High-throughput screening Agar plate pouring Colony imaging & analysis

9 About us For over eighty years, Singer Instruments have been designing and manufacturing cutting-edge scientific research equipment. No one understands the needs of yeast researchers and yeast micromanipulation better than Singers. From the development of ready-made needles, the automatic MSM 400, the MicroZapper spore agitator and purpose-made XLWD objectives, to the ROTOR HDA personal omics robot, no one has done more to make the yeast model accessible to all. From its headquarters in the beautiful Exmoor National Park in Somerset, UK, Singer ships micromanipulation systems and associated products internationally. World-leaders in instrumentation for yeast, Singer Instruments are working hard to make continual improvements to help assist the scientific community. singerinstruments.com

11 TM Colony Imaging with powerful Analysis Software TM singerinstruments.com

12 Roadwater, Watchet, Somerset. TA23 0RE +44 (0) (tel) +44 (0) (fax) singerinstruments.com Scan to visit OUR website Our distributors For a list of our distributors visit: bit.ly/singerdistributors

Colony Imaging with powerful Analysis Software

Colony Imaging with powerful Analysis Software TM Imaging with powerful Analysis Software TM Accurate Compact Fast We re not going to interpret your results, but we ll do everything to get you there From image acquisition to data visualisation, straight