The thin wall ESI tip appears unusually. advantageous for ensuring stable ESI. conditions and efficient ion transfers.

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2 The thin wall ESI tip appears unusually advantageous for ensuring stable ESI conditions and efficient ion transfers. Valaskovic, Kelleher, McLafferty Science (1996), 273, 1199

3 perfect zero-dead-volume PicoClear technology revolutionizes the way capillary connections are made. High performance applications that require connections with inline filters, liquid-junction voltage, sample-trap, or tees and crosses are now simple and easy! The PicoClear line of connectors can do it all even at high pressure.

4 How does PicoClear technology work? PicoClear connectors feature pure radial compression to provide a high performance connection. This novel format ensures the perfect alignment and union of fused silica tubing every time. Uniformly applied pressure surrounds the tubing, thereby eliminating friction and cracking that can lead to unwanted contaminants and result in a clogged nanospray emitter. Pure radial compression The radial compression provided by PicoClear connectors is a revolutionary development in LC-MS and eliminates frusto-conical compression connections featured in traditional fittings. Troubleshooting becomes diagnostic and clogging is virtually eliminated. Clearly, a revolutionary solution Body Optically Clear Insert Fluoropolymer Core Because PicoClear connectors are clear, perfect contact is confirmed with a simple visual inspection. A clear solution to a difficult problem. 2º Nut 1º Nut 1º Nut 2º Nut Elastomer core The elastomer core provides the platform for PicoClear technology and allows for the easy visualization of the perfect connection. Compression of the elastomer core results in a clear view of the union, and of course, a perfect connection. 2º Nut 1º Nut Body Optically Clear Insert Fluoropolymer Core Longitudinal compression shell applies pure axial compression 1º Nut 2º Nut Slide the first piece of tubing into the union, positioning it halfway through the window Insert the second piece of tubing into the opposite side of the union. Gaps are clearly visible if present. Tighten the end fittings to seal and compress the union. Full compression results in higher optical clarity.

5 PicoCLEAR Connectors PicoClear TM Applications PicoClear TM technology is an essential part of the high-performance analytical laboratory. Capillary connections are now robust and simple. 2D SEPARATIONS & BEYOND Build your own, custom multi-phasic columns. Take advantage of PicoClear technology and the novel zero-deadvolume design to connect columns of differing or similar chemistries - with no loss in performance. It s easy! VISIBLE INLINE FILTER Integrate PicoClear technology with an IntegraFrit nanobore column to create an in-line, metal free filter A powerful combination. ZERO-DEAD-VOLUME LIQUID JUNCTION VOLTAGE Need high voltage capabilities? The PicoClear conductive union provides the same zero-dead-volume connection with liquid junction highvoltage capability. The application of high voltage is more stable and efficient. Expanded chromatographic regions of BSA tryptic digest chromatographic peaks. Injection: 100 fmol BSA, Flow rate: 300 nl/ min., Gradient: 2% - 50% B over 70 min. All connections made using PicoClear union. Fritted tubing Flow To column An injection of BSA (300 fmol) was analyzed using three different liquidjunction high-voltage connection devices. The same column and emitter were used for all data collection. 8.4 s 9.6 s 12.6 s A NL:2.40E7 NL:2.97E7 NL:6.98E6 Metal union NL: 7.80E AA NL:4.78E7 Unpacked IntegraFrit column (on left) coupled with fused silica tubing (right) using PicoClear union A) Intact 30 cm C18-packed PicoFrit column A) Standard metal union 10.8 s 10.2 s 13.8 s NL:2.20E7 NL:2.58E7 NL:7.11E6 B PEEK microtee B Carbon and other harmful particulates are clearly visible (after 2 hours) NL: 1.05E NL:5.61E B) A C18-packed 20 cm IntegraFrit coupled to a C18-packed 10 cm PicoFrit column B) PEEK microtee with a platinum wire 10.8 s 12.0 s 13.2 s 2.40E7 2.97E7 6.98E6 C PicoClear Conductive Union C NL:4.03E7 C) Two C18-packed 10 cm IntegraFrit columns coupled to a 10 cm PicoFrit column Inside the PicoClear conductive union, highly efficient voltage contact is made within the zero-dead-volume liquidjunction connection N L: 4.04E Tim e (m in) C) PicoClear conductive union 33.07

6 High-Performance. Cost-Efficient. Modern applications require the highest possible performance - sensitivity, selectivity, ruggedness. Use PicoClear TM connectors over and over again to ensure a perfect connection that is free of contaminants. A quick visual inspection of the PicoClear see-through elastomer core can confirm that your fluid path is in tip-top shape. When it is time to replace the PicoClear core, simply remove the used core and replace it with a new one. PicoClear Union PCU-360 PCU-150 Reusable body Set of reusable nuts / ferrules Replaceable clear inserts (2) Swaging tool Specifications For 360 µm OD and 150 µm OD tubing Length: 48mm - 50 mm Assembled ( ) Height: 8mm (0.31 ) Width: 9mm (0.35 ) Max. Pressure: 9,000 psi PicoClear Conductive Union PCCU-360 Reusable conductive body Set of reusable nuts / ferrules Replaceable clear conductive insert (1) Bore rejuvenation kit Specifications For 360 µm OD tubing Length: 48mm - 50 mm Assembled ( ) Height: 8mm (0.31 ) Width: 9mm (0.35 ) Max. Pressure: 5,000 psi Learn more about PicoClear technology on our website: New Objective, Inc. All rights reserved. PicoClear U.S. and Foreign Patents Pending. PicoView, Digital PicoView, PicoFrit, IntegraFrit, PicoTip, GlassTip, EconoTip, QuartzTip, PicoClear, and ProteoPep are trademarks or registered trademarks of New Objective, Inc. All other trademarks are the property of their respective companies.

7 New Objective, Inc. Two Constitution Way Woburn, MA Orders Phone Fax newobjective.com

8 New Objective, Inc. Two Constitution Way Woburn, MA Orders Phone Fax newobjective.com