Lampiran 1.Perbedaan bentuk tubuh induk jantan & betina huna biru dengan huna capitmerah. Induk RR (huna capitmerah)

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1 L A M P I R A N 38

2 Lampiran 1.Perbedaan bentuk tubuh induk jantan & betina huna biru dengan huna capitmerah Tubuh Induk AA (Huna biru) Jantan Betina Induk RR (huna capitmerah) Jantan Betina 39

3 Lampiran 1 (lanjutan ) Perbedaan bentuk tubuh F1 jantan & betina antar perlakuan pada umur 5 bulan Tubuh Jantan Betina AA * * RA * * AR ** ** RR ** ** Keterangan : Symbol * & ** menunjukkan bentuk fenotifik yang sama untuk masingmasing jantan & betina (RA= AA) dan (AR = RR). 40

4 Lampiran 1(lanjutan). Perbedaan bentuk capit jantan & betina antar perlakuan pada umur 5 bulan Capit Jantan Betina AA * * RA * * AR ** ** RR ** Keterangan : Symbol * & ** menunjukkan bentuk fenotifik yang sama pada masing-masing perlakuan jantan & betina. Symbol menunjukkan bentuk capit jantan RR & AR bagian luar. ** 41

5 Lampiran 2. RAPD Hibrid huna capit merah dengan huna biru menggunakan primer OPA-8 Marker AA RA AR RR 42

6 Lampiran 3. Hasil analisa karakter fenotip F1 umur 5 bulan dan 9 bulan menggunakan ANOVA dengan prosedur SPSS 11,5. Umur 5 bulan Oneway Test of Homogeneity of Variances Levene Statistic df1 df2 Sig. PJGTOTAL PJGSTDR PJGKPL BERAT ANOVA PJGTOTAL PJGSTDR PJGKPL BERAT Sum of Squares Mean Square F Sig. df Total Total Total Total

7 Lampiran 3 (lanjutan). Post Hoc Tests Homogeneous Subsets PJGTOTAL POPULASI N Subset for alpha = Sig a Uses Harmonic Mean Sample Size = PJGSTDR POPULASI N Subset for alpha = Sig a Uses Harmonic Mean Sample Size =

8 Lampiran 3 (lanjutan). PJGKPL POPULASI N Subset for alpha = Sig a Uses Harmonic Mean Sample Size = BERAT POPULASI N Subset for alpha = Sig a Uses Harmonic Mean Sample Size = Test of Homogeneity of Variances Levene Statistic df1 df2 Sig. PJGCAPIT LBRCPT

9 Lampiran 3 (lanjutan). ANOVA PJGCAPIT LBRCPT Sum of Squares Mean Square F Sig. df Total Total PJGCAPIT Subset for alpha = PPOPUL.05 AS N Sig a Uses Harmonic Mean Sample Size =

10 Lampiran 3 (lanjutan). LBRCPT POPULAS1 N Subset for alpha = Sig a Uses Harmonic Mean Sample Size = Keterangan : Populasi 1.00 = RA Populasi 2.00 = AA Populasi 3.00 = AR Populasi 4.00 = RR 47

11 Lampiran 3 (lanjutan). Umur 9 bulan ANOVA PJGTOT PJGSTD PJGKPL BRT PJGCPT LBRCPT Sum of Squares Mean Square F Sig. df Total Total Total Total Total Total

12 Lampiran 3 (lanjutan) PJGTOT Subset for alpha = PRLK.05 N N 1 2 AA RR RA AR Sig a Uses Harmonic Mean Sample Size = PRLK N PJGSTD Subset for alpha = N AA RR AR RA Sig a Uses Harmonic Mean Sample Size = PRLK N PJGKPL Subset for alpha = N AA RR AR RA Sig a Uses Harmonic Mean Sample Size =

13 Lampiran 3 (lanjutan). BRT PRLK N Subset for alpha = N AA RR RA AR Sig a Uses Harmonic Mean Sample Size = PJGCPT Subset for alpha = PRLK.05 N N 1 2 RR AA AR RA Sig a Uses Harmonic Mean Sample Size = LBRCPT Subset for alpha = PRLK.05 N N 1 2 AA RA AR RR Sig a Uses Harmonic Mean Sample Size =

14 Lampiran 4. Hasil Analisa Karakter Genetik Dengan RAPD 6/17/2009 5:48:55 AM Analysis of C:\TFPGA\IRIN8.DAT Data set contains genotypes of individuals sampled from populations. Organism Type: Diploid Marker Type: Dominant H-W Equilibrium Assumed. Allele frequencies estimated based on the square root of the frequency of the null (recessive) genotype. DESCRIPTIVE STATISTICS ******************************************************** RESULTS FOR EACH POPULATION. POPULATION 1 = AA esults over all loci Ave. sample size: Ave. heterozygosity: Ave. heterozygosity (unbiased): Ave. heterozygosity (direct count): % polymorphic loci (no criterion): % polymorphic loci (99% criterion): % polymorphic loci (95% criterion): POPULATION 2 = RR Results over all loci Ave. sample size: Ave. heterozygosity: Ave. heterozygosity (unbiased): Ave. heterozygosity (direct count): % polymorphic loci (no criterion): % polymorphic loci (99% criterion): % polymorphic loci (95% criterion):

15 Lampiran 4 (lanjutan). POPULATION 3 = AR Results over all loci Ave. sample size: Ave. heterozygosity: Ave. heterozygosity (unbiased): Ave. heterozygosity (direct count): % polymorphic loci (no criterion): % polymorphic loci (99% criterion): POPULATION 4 = RA Results over all loci Ave. sample size: Ave. heterozygosity: Ave. heterozygosity (unbiased): Ave. heterozygosity (direct count): % polymorphic loci (no criterion): % polymorphic loci (99% criterion): % polymorphic loci (95% criterion): /17/2009 5:54:19 AM Analysis of C:\TFPGA\IRIN8.DAT Data set contains genotypes of individuals sampled from populations. Organism Type: Diploid Marker Type: Dominant H-W Equilibrium Assumed. Allele frequencies estimated based on the square root of the frequency of the null (recessive) genotype. 52

16 Lampiran 4 (lanjutan). GENETIC DISTANCES NEI'S (1972/1978) IDENTITIES/DISTANCES Nei's original (1972) identity Population ***** ***** ***** ***** Nei's original (1972) distance Population ***** ***** ***** ***** Nei's unbiased (1978) identity Population ***** ***** ***** ***** Nei's unbiased (1978) distance Population ***** ***** ***** ***** 6/17/2009 5:54:41 AM Analysis of C:\TFPGA\IRIN8.DAT Data set contains genotypes of individuals sampled from populations. Organism Type: Diploid Marker Type: Dominant H-W Equilibrium Assumed. Allele frequencies estimated based on the square root of the frequency of the null (recessive) genotype. 53

17 Lampiran 4 (lanjutan). UPGMA Cluster using Nei's (1972) original distance Node Distance Includes Populations KET : Populasi 1 = AA Populasi 2 = RR Populasi 3 = AR Populasi 4 = RA 6/17/2009 5:56:14 AM Analysis of C:\TFPGA\IRIN8.DAT Data set contains genotypes of individuals sampled from populations. Organism Type: Diploid Marker Type: Dominant H-W Equilibrium Assumed. Allele frequencies estimated based on the square root of the frequency of the null (recessive) genotype. Exact tests for population differentiation (Raymond and Rousset 1995) # of dememorization steps: 1000 # of batches: 10 # of permutations per batch: 2000 Matrix of combined probabilities for each pairwise comparison ***** ***** ***** ***** 54