essentials Western blotting -15% -15% -25% -20% -25% Up to -60% -20% -30% Mix-and-match and get exclusive year end discounts on TruPAGE precast gels

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1 Western blotting essentials Mix-and-match and get exclusive year end discounts -15% on Amicon Ultra centrifugals -25% on Roche inhibitor cocktail tablets -15% on TruPAGE precast gels -20% on Immobilon PVDF membrane rolls -25% on Western blotting reagents Up to -60% on basic Sigma-Aldrich biochemicals -30% on SNAP i.d. 2.0 system for fast immunodetection -20% on BRAND electronic pipettes and tips Order at using PROMO CODE HLF until 30/11/18. The life science business of Merck operates as MilliporeSigma in the U.S. and Canada.

2 ReThink Western Blotting We are pleased to offer an extensive selection of reagents, consumables and equipment to enable uncomplicated, reliable and fast protein preparation, electrophoresis, transfer, and immunoblotting. For a complete overview of our products for western blotting and useful tips & tricks to achieve better reproducibility, signal-to-noise ratio & robustness of your blots visit: SPECIAL OFFER WESTERN BLOTTING ESSENTIALS The Western Blotting Essentials promotion is valid from September, 1st till November, 30th 2018 for orders placed via the Sigma-Aldrich e-shop or customer center. Only discount levels are indicated on this promotional flyer, for prices in your local currency, visit Sigma-Aldrich e-shop and use special Promotion Code: HLF when ordering. Offer valid in Eastern Europe & Russia+ territory throughout the validity period stated. Certain countries may be excluded - please contact your local representative for information specific to your region. Offer doesn t apply to products sold through or dealers or to customers with contracts in place. Offer may not be combined with any other offer or promotion. We reserve the right to modify or terminate promotion at any time in its sole discretion. Should you have any questions, please contact your sales representative or our local office: PRODUCT HIGHLIGHT NEW IMMOBILON -E MEMBRANE Hydrophobic PVDF transfer membranes offer superior retention of proteins and higher durability than conventional nitrocellulose. These properties deliver higher sensitivity in western blotting and more robust results in demanding protocols like those for stripping and reprobing. However, you must pre-wet PVDF membranes in alcohol before use. Until now! Immobilon -E membrane is the first PVDF transfer membrane on the market that wets with aqueous transfer buffer and offers the benefits of PVDF without an alcohol pre-wet step. It is part of this special offer with cat. number IEVH Immobilon -E membrane Nitrocellulose membrane HSP70 GADPH The life science business of Merck operates as MilliporeSigma in the U.S. and Canada. 2

3 WESTERN BLOTTING ESSENTIALS - LIST OF DISCOUNTED PRODUCTS PROMO CODE HLF VALIDITY 01/09/18-30/11/18 TO ORDER GO TO Immobilon PVDF transfer membranes in economical packaging in rolls IPVH00010 Immobilon-P PVDF Membrane 1 roll, 26.5 cm x 3.75 m 20% ISEQ00010 Immobilon-PSQ PVDF Membrane 1 roll, 26.5 cm x 3.75 m 20% IPFL00010 Immobilon-FL PVDF Membrane 1 roll, 26.5 cm x 3.75 m 20% IPVH00005 Immobilon-P PVDF Membrane 1 roll, 26.5 cm x m 20% ISEQ00005 Immobilon-PSQ PVDF Membrane 1 roll, 26.5 cm x m 20% IPFL00005 Immobilon-FL PVDF Membrane 1 roll, 26.5 cm x m 20% IEVH00005 Immobilon-E PVDF Membrane NEW NO NEED OF ALCOHOL PREWETTING!!! 1 roll, 26.5 cm x m 20% SNAP i.d. 2.0 vacuum system for fast immunodetection SNAP2MINI SNAP i.d. 2.0 Protein Detection System-Mini (7.5 x 8.4 cm) 1 kit 30% SNAP2MIDI SNAP i.d. 2.0 Protein Detection System-Midi (8.5 x 13.5 cm) 1 kit 30% SNAP2MB3 SNAP i.d. 2.0 Protein Detection System-Double MultiBlot (4.5 x 8.4 cm) 1 kit 30% SNAP2MM SNAP2MB1 SNAP2MB2 SNAP i.d. 2.0 Protein Detection System-Mini and Midi (7.5 x 8.4 cm and 8.5 x 13.5 cm) SNAP i.d. 2.0 Protein Detection System-Mini and MultiBlot (7.5 x 8.4 cm and 4.5 x 8.4 cm) SNAP i.d. 2.0 Protein Detection System-Midi and MultiBlot (8.5 x 13.5 cm and 4.5 x 8.4 cm) Luminata ready-to-use premixed HRP substrates 1 kit 30% 1 kit 30% 1 kit 30% WBLUC0100 Luminata Classico Western HRP substrate 100 ml 25% WBLUC0500 Luminata Classico Western HRP substrate 500 ml 25% WBLUR0100 Luminata Crescendo Western HRP substrate 100 ml 25% WBLUR0500 Luminata Crescendo Western HRP substrate 500 ml 25% WBLUF0100 Luminata Forte Western HRP substrate ROOM TEMPERATURE STORAGE 100 ml 25% WBLUF0500 Luminata Forte Western HRP substrate ROOM TEMPERATURE STORAGE 500 ml 25% Most flexible Immobilon HRP substrates WBKLS0050 Immobilon Western Chemiluminescent HRP Substrate 2 x 25 ml 25% WBKLS0100 Immobilon Western Chemiluminescent HRP Substrate 2 x 50 ml 25% WBKLS0500 Immobilon Western Chemiluminescent HRP Substrate 2 x 250 ml 25% WBULS0100 WBULS0500 Immobilon ECL Ultra Western HRP Substrate NEW FOR MID-TO-LOW FEMTOGRAM RANGE Immobilon ECL Ultra Western HRP Substrate NEW FOR MID-TO-LOW FEMTOGRAM RANGE Ultrastrong composite precast gels with extended 2 year shelf life 2 x 50 ml 25% 2 x 250 ml 25% PCG2001-2EA TruPAGE Precast Gels 10%, 10 x 10cm, 12-well 2 pack 15% PCG EA TruPAGE Precast Gels 10%, 10 x 10cm, 12-well 10 pack 15% PCG2005-2EA TruPAGE Precast Gels 10%, 10 x 10cm, 17-well 2 pack 15% PCG EA TruPAGE Precast Gels 10%, 10 x 10cm, 17-well 10 pack 15% PCG2009-2EA TruPAGE Precast Gels 10%, 10 x 8cm, 12-well 2 pack 15% PCG EA TruPAGE Precast Gels 10%, 10 x 8cm, 12-well 10 pack 15% PCG2013-2EA TruPAGE Precast Gels 10%, 10 x 8cm, 17-well 2 pack 15% PCG EA TruPAGE Precast Gels 10%, 10 x 8cm, 17-well 10 pack 15% PCG2002-2EA TruPAGE Precast Gels 12%, 10 x 10cm, 12-well 2 pack 15% PCG EA TruPAGE Precast Gels 12%, 10 x 10cm, 12-well 10 pack 15% PCG2006-2EA TruPAGE Precast Gels 12%, 10 x 10cm, 17-well 2 pack 15% 3

4 PCG EA TruPAGE Precast Gels 12%, 10 x 10cm, 17-well 10 pack 15% PCG2010-2EA TruPAGE Precast Gels 12%, 10 x 8cm, 12-well 2 pack 15% PCG EA TruPAGE Precast Gels 12%, 10 x 8cm, 12-well 10 pack 15% PCG2014-2EA TruPAGE Precast Gels 12%, 10 x 8cm, 17-well 2 pack 15% PCG EA TruPAGE Precast Gels 12%, 10 x 8cm, 17-well 10 pack 15% PCG2003-2EA TruPAGE Precast Gels 4-12%, 10 x 10cm, 12-well 2 pack 15% PCG EA TruPAGE Precast Gels 4-12%, 10 x 10cm, 12-well 10 pack 15% PCG2007-2EA TruPAGE Precast Gels 4-12%, 10 x 10cm, 17-well 2 pack 15% PCG EA TruPAGE Precast Gels 4-12%, 10 x 10cm, 17-well 10 pack 15% PCG2011-2EA TruPAGE Precast Gels 4-12%, 10 x 8cm, 12-well 2 pack 15% PCG EA TruPAGE Precast Gels 4-12%, 10 x 8cm, 12-well 10 pack 15% PCG2015-2EA TruPAGE Precast Gels 4-12%, 10 x 8cm, 17-well 2 pack 15% PCG EA TruPAGE Precast Gels 4-12%, 10 x 8cm, 17-well 10 pack 15% PCG2004-2EA TruPAGE Precast Gels 4-20%, 10 x 10cm, 12-well 2 pack 15% PCG EA TruPAGE Precast Gels 4-20%, 10 x 10cm, 12-well 10 pack 15% PCG2008-2EA TruPAGE Precast Gels 4-20%, 10 x 10cm, 17-well 2 pack 15% PCG EA TruPAGE Precast Gels 4-20%, 10 x 10cm, 17-well 10 pack 15% PCG2012-2EA TruPAGE Precast Gels 4-20%, 10 x 8cm, 12-well 2 pack 15% PCG EA TruPAGE Precast Gels 4-20%, 10 x 8cm, 12-well 10 pack 15% PCG2016-2EA TruPAGE Precast Gels 4-20%, 10 x 8cm, 17-well 2 pack 15% PCG EA TruPAGE Precast Gels 4-20%, 10 x 8cm, 17-well 10 pack 15% PCG3009-1ML TruPAGE LDS Sample Buffer 1 ML 15% PCG ML TruPAGE LDS Sample Buffer 10 ML 15% PCG ML TruPAGE TEA-Tricine SDS Running Buffer 50 ML 15% PCG ML TruPAGE TEA-Tricine SDS Running Buffer 500 ML 15% PCG3001-4L TruPAGE TEA-Tricine SDS Running Buffer 4 L 15% PCG ML TruPAGE Tris-MOPS SDS Express Running Buffer 500 ML 15% PCG3003-4L TruPAGE Tris-MOPS SDS Express Running Buffer 4 L 15% PCG3005-1ML TruPAGE DTT Sample Reducer 1 ML 15% PCG ML TruPAGE Running Antioxidant 10 ML 15% Industry leading ultrafiltration centrifugals with lowest binding RC membranes UFC Amicon Ultra-0.5 Centrifugal Filter Unit 3 kda 24 pack 15% UFC Amicon Ultra-0.5 Centrifugal Filter Unit 10 kda 24 pack 15% UFC Amicon Ultra-0.5 Centrifugal Filter Unit 30 kda 24 pack 15% UFC Amicon Ultra-0.5 Centrifugal Filter Unit 50 kda 24 pack 15% UFC Amicon Ultra-0.5 Centrifugal Filter Unit 100 kda 24 pack 15% UFC Amicon Ultra-2 Centrifugal Filter Unit 3 kda 24 pack 15% UFC Amicon Ultra-2 Centrifugal Filter Unit 10 kda 24 pack 15% UFC Amicon Ultra-2 Centrifugal Filter Unit 30 kda 24 pack 15% UFC Amicon Ultra-2 Centrifugal Filter Unit 50 kda 24 pack 15% UFC Amicon Ultra-2 Centrifugal Filter Unit 100 kda 24 pack 15% UFC Amicon Ultra-4 Centrifugal Filter Unit 3 kda 24 pack 15% UFC Amicon Ultra-4 Centrifugal Filter Unit 10 kda 24 pack 15% UFC Amicon Ultra-4 Centrifugal Filter Unit 30 kda 24 pack 15% UFC Amicon Ultra-4 Centrifugal Filter Unit 50 kda 24 pack 15% UFC Amicon Ultra-4 Centrifugal Filter Unit 100 kda 24 pack 15% UFC Amicon Ultra-15 Centrifugal Filter Unit 3 kda 24 pack 15% UFC Amicon Ultra-15 Centrifugal Filter Unit 10 kda 24 pack 15% UFC Amicon Ultra-15 Centrifugal Filter Unit 30 kda 24 pack 15% UFC Amicon Ultra-15 Centrifugal Filter Unit 50 kda 24 pack 15% UFC Amicon Ultra-15 Centrifugal Filter Unit 100 kda 24 pack 15% 4

5 Conveniet fast and no smell stripping solution 2502 ReBlot Plus Mild Antibody Stripping Solution 50 ml 25% 2504 ReBlot Plus Strong Antibody Stripping Solution 50 ml 25% Best selling Roche inhibitor cocktail tables packed in blister foils complete Protease Inhibitor Cocktail 20 tablets in EASYpack 25% complete, Mini Protease Inhibitor Cocktail 30 tablets in EASYpack 25% complete, EDTA-free Protease Inhibitor Cocktail 20 tablets in EASYpack 25% complete, Mini, EDTA-free Protease Inhibitor Cocktail 30 tablets in EASYpack 25% complete ULTRA Tablets, Mini, EDTA-free, EASYpack Protease Inhibitor Cocktail 30 tablets in EASYpack 25% complete ULTRA Tablets, Mini, EASYpack Protease Inhibitor Cocktail 30 tablets in EASYpack 25% PhosSTOP 10 tablets in EASYpack 25% PhosSTOP 20 tablets in EASYpack 25% NEW "COMBO" inhibitor cocktails in ready-to-use DMSO solution format PPC1010-1ML PPC1010-5ML Protease and Phosphatase Inhibitor Cocktail NEW INHIBITS BOTH PROTEASES AND PHOSPHATASES Protease and Phosphatase Inhibitor Cocktail NEW INHIBITS BOTH PROTEASES AND PHOSPHATASES Ready-to-use acrylamide/bis-acryalmide solutions 1 ML RTU solution in DMSO 5 ML RTU solution in DMSO A3699-5X100ML A ML A3574-5X100ML A ML A9926-5X100ML A7802-5X100ML A ML Acrylamide/Bis-acrylamide, 30% solution, BioReagent, suitable for electrophoresis, 37.5:1 Acrylamide/Bis-acrylamide, 30% solution, BioReagent, suitable for electrophoresis, 37.5:1 Acrylamide/Bis-acrylamide, 30% solution, BioReagent, suitable for electrophoresis, 29:1 37.5:1 19:1 29:1 29:1 Basic biochemicals (not only) for electrophoresis of proteins 25% 25% 100 ml 40% 100 ml 40% 100 ml 40% I ML 2-Propanol, for molecular biology, BioReagent, 99.5% 500 ml 50% B4429-1KG BIS-TRIS, BioPerformance Certified, cell culture tested, suitable for insect cell culture, 98.0% 1 kg 40% D G DL-Dithiothreitol, for electrophoresis, 99% 25 g 20% D9163-5G DL-Dithiothreitol, for electrophoresis, 99% 5 g 20% X.5ML DL-Dithiothreitol solution, 1 M in H 2 O 10 x 0.5 ml 30% E5134-1KG Ethylenediaminetetraacetic acid disodium salt dihydrate, for electrophoresis, for molecular biology, % (titration) 1 kg 60% G8898-1KG Glycine, for electrophoresis, 99% 1 kg 40% G G Glycine, for electrophoresis, 99% 500 g 40% G ML Guanidine hydrochloride solution, 8 M, ph 8.5, buffered aqueous solution 100 ml 30% H ML Hydrochloric acid, %, BioReagent, for molecular biology 500 ml 60% P PAK Phosphate buffered saline, powder, ph PAK 40% P3813-5X10PAK Phosphate buffered saline, powder, ph x 10 PAK 30% S VL Sample Buffer, Laemmli 2 Concentrate 10 vials per 6 ml 30% T G Trichloroacetic acid, BioXtra, 99.0% 500 g 30% X1ML Tris(2-carboxyethyl)phosphine hydrochloride solution, 0.5 M, ph 7.0 (aqueous solution; ph was adjusted with ammonium hydroxide) 10 x 1 ml 30% T4904-1L Tris-Glycine Buffer 10 Concentrate 1 l 30% 5

6 T6066-5KG T5941-1KG Trizma base, BioPerformance Certified, meets EP, USP testing specifications, cell culture tested, 99.9% (titration) Trizma hydrochloride, BioPerformance Certified, cell culture tested, 99.0% (titration) 5 kg 40% 1 kg 40% U4883-6X25ML Urea, 8 M (after reconstitution with 16 ml high purity water) 6 x 25 ml 15% U6504-1KG Urea, for electrophoresis 1 kg 40% W4502-1L Water, Molecular Biology Reagent 1 l 40% W4502-6X1L Water, Molecular Biology Reagent 6 x 1 l 25% Ergonomic, single-channel electronic micropipettes with a patented gel loading mode Z EA BRAND Transferpette electronic pipette, single channel, capacity μl 1 pc 20% Z EA BRAND Transferpette electronic pipette, single channel, capacity 2-20 μl 1 pc 20% Z EA BRAND Transferpette electronic pipette, single channel, capacity μl 1 pc 20% Z EA BRAND pipette tips, racked, TipRack, capacity 1-50 µl, non-sterile 10 racks of 96 20% Z EA BRAND pipette tips, racked, TipRack, capacity µl, non-sterile 10 racks of 96 20% Z EA BRAND pipette tips, racked, TipBox, capacity µl, non-sterile 5 boxes of 96 20% Z EA BRAND pipette tips, racked, TipStack, capacity µl, non-sterile 10 racks of boxes 20% Z EA BRAND pipette tips, racked, TipRack, capacity µl, non-sterile 10 racks of 96 20% Z EA BRAND pipette tips, racked, TipBox, capacity µl, non-sterile 5 boxes of 96 20% View additional product information, applications, real-time availability, ordering and contact information at SigmaAldrich.com The life science business of Merck operates as MilliporeSigma in the U.S. and Canada Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved. Merck and the vibrant M are trademarks of Merck KGaA, Darmstadt, Germany or its affiliates. All other trademarks are the property of their respective owners. Detailed information on trademarks is available via publicly accessible resources.