New Amersham Hyperfilm

Transcription

1 GE Healthcare The clear choice for reliable results With Comparative Data New Amersham Hyperfilm

2 Are you looking for the right film with the clearest results? As a scientist with a passion for research, you deserve to use the best scientific tools to succeed. From chemiluminescent to radioactive detection, it's important to ensure you have the right film for the job a film that offers excellent sensitivity, band clarity and artifact-free, publication-quality images; as well as excellent value for money and the potential for significant cost savings, to make your research budget go further. For over 20 years, Amersham Hyperfilm has remained the film brand researchers in the life sciences have come to trust. When you compare the results generated by Hyperfilm ECL in chemiluminescent and Hyperfilm MP in radioactive detections with Kodak BioMax films...you will clearly see the difference.

3 the most sensitive chemiluminescence detection film Applications: chemiluminescent detection in Western, Southern and Northern blots Increased sensitivity: greater sensitivity for low concentration protein detection Clearer background: superior contrast and band visibility in the detection of chemiluminescent signals Artifact-free results: minimal occurrence of visual artifacts due to the improved anti-static layer Validated performance: compatibility with ECL, ECL Plus, ECL Advance, CDP-Star and all blue and green chemiluminesence systems compared with Kodak BioMax Light Increased sensitivity and superior contrast Kodak BioMax Light Validated for use with commonly used chemiluminescent reagents A B Fig 1. Western blot detection with Kodak BioMax Light and films. Performance of and Kodak BioMax Light with Western blots of immobilized IgG from mouse protein (5 µg, 2.5 µg, 1.25 µg, 635 ng, 310 ng, 155 ng, 77 ng, and 39 ng). Detected with horse radish peroxidase labeled anti-mouse antibody 1:5000 and Amersham ECL Western Blot detection reagents. C D Publication quality images Kodak BioMax Light Fig 3. A. Western blot of co-immunoprecipitated p53 detected with Amersham ECL detection reagents. Result A kindly supplied by Pamela Robertson, Dept. of Surgery and Molecular Oncology, University of Dundee, UK. B. Western blot of Rabbit IgG detected with Amersham ECL Plus detection reagents. C. Western blot of Rabbit IgG detected with Amersham ECL Advance detection reagents. D. Southern blot of human genomic DNA, detected with n-ras insert probe labeled with Amersham AlkPhos Direct and detected with CDP-Star detection reagents. Fig 2. Southern blot CDP-Star detection with Kodak BioMax Light and Amersham Hyperfilm ECL films. Southern blot of human genomic DNA (1, 0.5, 0.25, 0.12, 0.06, 0.03 µg per lane) detected with n-ras insert probe labeled with Amersham AlkPhos Direct and detected with CDP-Star detection reagents.

4 the clearest results in radioactive detection Applications: 32 P, 33 P, 125 I Northern, Southern and Western blots 35 S, 33 P, 32 P sequencing Fluorography of 3 H, 14 C, and 35 S High sensitivity: sensitivity down to single-copy gene detection Clearer results: a clearer, colorless background which improves band visibility Flexibility: compatibility for use with all commonly used radioisotopes compared with Kodak BioMax X-Omat AR-5 Superior contrast Kodak BioMax X-Omat AR-5 Excellent sensitivity Kodak BioMax X-Omat AR-5 dpm/band Fig 4. Comparative use of Kodak Biomax X-Omat AR-5 and in Southern blot 32 P detection. Single-copy gene detection using 32 P Southern blot of human genomic DNA isolated from whole blood and digested with HindIII (1, 0.5, 0.25, 0.12, 0.06, 0.03 µg per lane). Detection with n-ras insert probe (1.5 kb) labeled with 32 P- dctp. Autoradiography with at -70ºC with two Amersham Hyperscreen Intensifying Screens for 18 h Fig 5. Comparative use of Kodak BioMax X-Omat AR-5 and in dot blots of 32 P (1991, 995, 796, 398, 199, 99.5, 39.82, dpm) exposed to each film for 16 h.

5 Amersham Hyperfilm for high-sensitivity applications High sensitivity: Sensitivity down to single-copy gene detection Speed: Rapid results for when experimental length needs to be kept to a minimum. Whether using chemiluminescent or radioactive detection, high sensitivity is critical for experiments with low target concentrations, low-intensity samples or where experimental length needs to be kept to a minimum. compared with Kodak BioMax MS Increased sensitivity compared with Kodak BioMax MS Excellent performance without a premium price Kodak BioMax MS BioMax MS dpm/band Fig 9. Western blot analysis with Kodak BioMax MS and films using rabbit IgG (155, 75, 37.5, 18.75, 9.37, 4.7, 2.34, 1.17, 0.58, 0.3 ng) and Amersham ECL Advance detection reagents 5 min exposure Fig 10. Comparative use of Kodak BioMax MS and in dot blot experiments using 32 P (400, 200, 100, 50, 25, 12.5, 6.25, 3.125, 1.56, 0.78 dpm) with an exposure time of 24 h.

6 Amersham Hyperfilm for high resolution applications High resolution: excellent band resolution to visualize fine detail and detect low intensity bands High sensitivity: no compromise on sensitivity Flexibility: compatible for use with all radioisotopes compared with Kodak BioMax MR No compromise on sensitivity Excellent resolution with 33 P BioMax MR dpm/band 1991 Fig 7. Cycle sequencing using Redivue nucleotides ( 33 P) with an overnight exposure to Fig 6. Application of Kodak BioMax MR and Hyperfilm MP to dot blots of 32 P (1991, 995, 796, 398, 199, 99.5, 39.82, and dpm). Exposure time was 16 h for both films. Excellent resolution with 35 S Fig 8. Dideoxy sequence using Redivue Nucleotides ( 35 S) with an overnight exposure to.

7 Ordering Information Kodak BioMax Light vs. Kodak Kodak Equivalent Hyperfilm code Hyperfilm code Product code Hyperfilm Product 50 sheets 100 sheets BioMax Light-1, 18 x 24 cm, 50 Sheets Hyperfilm ECL, 18 x 24 cm BioMax Light-1, 24 x 30 cm, 50 Sheets Hyperfilm ECL, 24 x 30 cm BioMax Light-1, 35 x 43 cm, 50 Sheets Hyperfilm ECL, 35 x 43 cm BioMax Light-1, 5 x 7 in, 50 Sheets Hyperfilm ECL, 5 x 7 in BioMax Light-1, 8 x 10 in, 50 Sheets Hyperfilm ECL, 8 x 10 in Kodak BioMax X-Omat AR-5 vs. Kodak Kodak Equivalent Hyperfilm code Hyperfilm code Product code Hyperfilm Product 50 sheets 100 sheets BioMax X-Omat AR-5, 18 x 24 cm, 50 Sheets Hyperfilm MP, 18 x 24 cm BioMax X-Omat AR-5, 24 x 30 cm, 50 Sheets Hyperfilm MP, 24 x 30 cm BioMax X-Omat AR-5, 35 x 43 cm, 50 Sheets Hyperfilm MP, 35 x 43 cm BioMax X-Omat AR-5, 8 x 10 in, 50 Sheets Hyperfilm MP 8 x 10 in BioMax X-Omat AR-5, 5 x 7 in, 50 Sheets Hyperfilm MP, 5 x 7 in Hyperfilm MP, 18 x 24 cm (enveloped) Kodak MS vs. and MP Kodak Kodak Equivalent Hyperfilm code Hyperfilm code Product code Hyperfilm Product 50 sheets 100 sheets BioMax MS-1, 18 x 24 cm, 50 Sheets BioMax MS-1, 24 x 30 cm, 50 Sheets BioMax MS-1, 35 x 43 cm, 50 Sheets BioMax MS-1, 8 x 10 in, 50 Sheets BioMax MS-1, 5 x 7 in, 50 Sheets V V V V V Hyperfilm ECL, 18 x 24 cm Hyperfilm MP, 18 x 24 cm Hyperfilm ECL, 24 x 30 cm Hyperfilm MP, 24 x 30 cm Hyperfilm ECL, 35 x 43 cm Hyperfilm MP, 35 x 43 cm Hyperfilm ECL, 8 x 10 in Hyperfilm MP, 8 x 10 in Hyperfilm ECL, 5 x 7 in Hyperfilm MP, 5 x 7 in Hyperfilm MP, 18 x 24 cm (enveloped) Kodak MR vs. Kodak Kodak Equivalent Hyperfilm code Hyperfilm code Product code Hyperfilm Product 50 sheets 100 sheets BioMax MR-1, 18 x 24 cm, 50 Sheets Hyperfilm MP, 18 x 24 cm BioMax MR-1, 24 x 30 cm, 50 Sheets Hyperfilm MP, 24 x 30 cm BioMax MR-1, 35 x 43 cm, 50 Sheets Hyperfilm MP, 35 x 43 cm BioMax MR-1, 8 x 10 in, 50 Sheets Hyperfilm MP 8 x 10 in BioMax MR-1, 5 x 7 in, 50 Sheets Hyperfilm MP, 5 x 7 in Hyperfilm MP, 18 x 24 cm (enveloped)

8 GE, imagination at work and GE monogram are trademarks of General Electric Company. Amersham, Hyperfilm, ECL, ECL Advance, ECL Plus and Redivue are trademarks of GE Healthcare companies. Kodak, BioMax, and X-Omat are trademarks of Eastman Kodak Co. CDP-Star is a trademark of Tropix Inc General Electric Company All rights reserved. First published March 2007 All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them. A copy of these terms and conditions is available on request. Contact your local GE Healthcare representative for the most current information. GE Healthcare Bio-Sciences AB Björkgatan 30, Uppsala Sweden GE Healthcare Europe, GmbH Munzinger Strasse 5, D Freiburg Germany GE Healthcare Bio-Sciences Corp. 800 Centennial Avenue, P.O. Box 1327 Piscataway, NJ USA GE Healthcare Bio-Sciences KK Sanken Bldg., , Hyakunincho Shinjuku-ku, Tokyo Japan Asia Pacific Tel: Fax: Australasia Tel: Fax: Austria Tel: Fax: Belgium Tel: Fax: Canada Tel: Fax: Central & East Europe Tel: Fax: Denmark Tel: Fax: Eire Tel: Fax: Finland & Baltics Tel: Fax: France Tel: Fax: Germany Tel: Fax: Greater China Tel: Fax: Italy Tel: Fax: Japan Tel: Fax: Korea Tel: Fax: Latin America Tel: Fax: Middle East & Africa Tel: Fax: Netherlands Tel: Fax: Norway Tel: Fax: Portugal Tel: Fax: Russia, CIS & NIS Tel: Fax: Spain Tel: Fax: Sweden Tel: Fax: Switzerland Tel: Fax: UK Tel: Fax: USA Tel: Fax: GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK AA 03/2007