LABOGENA. Genetic Analysis Laboratory for Animal Species. Group of Economic Interest Jouy en Josas 2004 LABOGENA - MYB 1
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1 LABOGENA Genetic Analysis Laboratory for Animal Species Group of Economic Interest Jouy en Josas 2004 LABOGENA - MYB 1
2 Between research and genetic improvement Initially derived from an INRA blood group lab, it was transformed in 1994 into a company to expand its activities LABOGENA is a commercial DNA genotyping lab, owned by INRA (43,3%) and its main customers of different species (particularly cattle, horse, and sheep) animal industry partners 40 years service activities in the genetics field 2004 MYB - LABOGENA 2
3 Shareholders Fr.UPRa S. 6,7% APCA 9% UNCEIA 6,7% SYSAAF 3,8% INRA 43,3% Inst. Elevage 9,6% Haras Nationaux 21,2% 2004 MYB - LABOGENA 3
4 Evolution of analyses blood typing molecular biology MYB - LABOGENA 4
5 Activities DNA extraction and storage for research programs, DNA library 2004 MYB - LABOGENA 5
6 Activities DNA extraction and storage for research programs, DNA library Genetic identification and parentage testing o with microsatellite markers including the Internationally recognised (ISAG) ones 2004 MYB - LABOGENA 6
7 Prélèvement ( sang, poil, sperme, viande...) Extraction de l ADN microsatellite M PCR A B Produits de la PCR Dépôt sur séquenceur automatique A B Microsatellite. M Traitement informatique des données Définition du génotype 2004 MYB - LABOGENA 7
8 international Certificat LABOGENA Laboratoire d'analyses génétiques pour les espèces animales G.I.E. Domaine de Vilvert JOUY-EN-JOSAS Cedex (FRANCE) TEL : (33) FAX : (33) DNA N N SIRE G Equine DNA Typing Card Name Yob Sex Breed Colour HORSE Sire Dam 1994 MALE SELLE Français BAY H X AHT4 AHT5 ASB2 HMS1 HMS3 HMS6 HMS7 HTG4 HTG6 HTG10 VHL20 *1 H M K O N M N O P O P N O K P O I L P R *2 H K M N K N J M N O M P L N M O J O N Q K N H O J K Q I J P L O N K J K N L N * 1.2 : ISAG 1998 Horse Comparison Test reference samples. Tested in 2001 Jouy-en-Josas, le 15/11/ MYB - LABOGENA 8
9 Parentage testing and assignation Species Analysis per year Blood Group electrophoresis from to Microsatellites from Nb of microsatellites Efficacy EP Dog Goat Sheep Turbot Swine Donkey Sea bass Gilthead bream Horse Truite Cattle Cat MYB - LABOGENA 9
10 Activities DNA extraction and storage for research programs, DNA library Large activity in genetic identification and parentage testing Genotyping activities research programs (gene mapping and QTL research for cattle,swine, poultry and sheep), population diversity marker-assisted selection (cattle), identified genes involved in production traits (caseins, double muscling ), genetic diseases or disease susceptibility (CVM, scrapie...) MYB - LABOGENA 10
11 Some studied genes gene specie Expression Kappa Casein Cattle milk CD 18 Cattle BLAD Myostatine Cattle Double muscling Extension Cattle & Equine Coat Colour LAMC2-LAMA3 Equine & Canine JEB RYR Swine PSS PrP Sheep Scrapie as1 Casein Goat milk 2004 MYB - LABOGENA 11
12 Double muscling F94L Q204X E226X NT419 NT821 C313Y S105C D182N E291X Exon 1 Intron 1 Exon 2 Intron 2 Exon MYB - LABOGENA 12
13 Double muscling : LAR technique S105C F94L NT821 Q204X NT419 E226X C313Y D182N E291X animal non culard Q204X Q204X animal hétérozygote Q204X Q204X animal homozygote Q204X 2004 MYB - LABOGENA 13
14 Double muscling genotype interpretation Breed C313Y E226X E291X F94L NT419 NT821 Q204X S105C D182N Interpretation 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 +/+ wild type Charolaise 1/1 1/1 1/1 1/1 1/1 1/1 1/2 1/1 1/1 mh/+ Heterozygote carrier mh Maine Anjou 1/1 1/1 1/1 1/1 1/1 1/1 2/2 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/2 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/1 1/2 1/1 1/1 1/1 1/1 1/1 1/2 1/1 1/1 1/2 1/1 1/1 1/1 1/1 mh/mh Homozygote carrier mh +/+ wild type mh/+ Heterozygote carrier mh mh/+ Heterozygote carrier mh mh/mh Homozygote carrier mh 1/1 2/2 1/1 1/1 1/1 1/1 1/1 1/1 1/1 mh/mh Homozygote carrier mh 1= wild allele 2= mutated allele 2004 MYB - LABOGENA 14
15 Scrapie Haplotype on PrP gene (3 codons) VRQ AHQ ARQ A (Ala) R (Arg) Q (Gln) V (Val) H (His) R (Arg) H (His) ARR > AHQ > ARQ = ARH > VRQ ARR ARH Resistance Sensitivity 2004 MYB - LABOGENA 15
16 11 years scrapie programs European research program dairy breed Selection National scrapie program codons PCR/RFLP Séquenceur automatique TaqMan samples analysed for 3 codons, SNPs MYB - LABOGENA 16
17 SNPs 2006 ( ) scrapie susceptibility 85,94% RN (swine) 0,55% PSS (swine) 1,96% Casein (goat) 1,14% double muscling 2,91% JEB (dog) 0,30% CVM 4,09% Casein (cattle) 0,31% BLAD 2,06% JEB (equine) 0,70% coat colour 0,05% 2004 MYB - LABOGENA 17
18 Activities per species Sw ine 7,8% Fishes 2,3% Goat 1,1% Poultry 0,4% Cattle 35,5% Sheep 34,1% Cat 0,1% Dog 0,7% Horse 18,0% 2004 MYB - LABOGENA 18
19 analyses in 2006 Identification- PT 42% DNA Libraries 6% Research-MAS 15% Genes 37% 2004 MYB - LABOGENA 19
20 Facilities & organisation 60 Engineers & technicians Facilities 1200 m2 laboratories Capacity to invest Up to date equipments Research & development unit 2004 MYB - LABOGENA 20
21 SNP genotyping Nb SNPs per individual Other? 0, SNPlex 0, LAR 1 Automatic technologies (costly consomable and devices) 1 PCR/RFLP 10 TaqMan 4 Nb individuals per year 2004 MYB - LABOGENA 21
22 Facilities & organisation 60 Engineers & technicians Facilities Research & development unit ISO 9001 Certification ISO Accreditation (in progress) National Reference laboratory (2002) for scrapie genotyping and Cattle genetic identification 2004 MYB - LABOGENA 22
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