USING GENETIC TESTING TO INFORM MANAGEMENT OF PHRAGMITES IN COLORADO. Patty York EDRR & List A Specialist Colorado Dept of Agriculture
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1 USING GENETIC TESTING TO INFORM MANAGEMENT OF PHRAGMITES IN COLORADO Patty York EDRR & List A Specialist Colorado Dept of Agriculture
2 THREE SUBSPECIES IN THE US Phragmites australis subspecies americanus Occurs as scattered plants within broader plant communities Phragmites australis subspecies berlandieri Also known as Gulf Coast Phragmites Phragmites australis subspecies australis Also known as Haplotype M Introduced to east coast U.S. by early 1800 s Gradually expanding westward Saltonstall et al SIDA 21(2):
3 COUNTY DISTRIBUTION ACCORDING TO EDDMAPS DATABASE European common reed Phragmites australis ssp. australis (Cavanilles) Trinius ex Steudel
4 COUNTY DISTRIBUTION ACCORDING TO EDDMAPS DATABASE Common reed Phragmites australis (Cavanilles) Trinius ex Steudel
5 BIOLOGY OF PHRAGMITES AUSTRALIS SPP. AUSTRALIS 6-15 foot tall perennial grass Spreads by rhizomes Forms dense thickets in or near water Blooms July to October Flowers feathery & drooping; can appear silvery or purplish Leaves blue-green, flat, smooth
6 IMPACTS OF PHRAGMITES Can negatively affect biodiversity and ecological functions Can impair the recreational use of wetlands and shorelines Can decrease property values Can increase fire risk
7 HISTORY OF PHRAGMITES IN COLORADO In 2010, site identified along Fountain Creek (13 th St. to Arkansas River) CDA Staff visited in 2010 during a controlled burn Plants tested, and confirmed invasive Placed on the Watch List in 2012 May also be native plants in area
8 HISTORY OF PHRAGMITES IN COLORADO 2013 Free Genetic Testing by Chicago Botanic Garden Only a handful of Colorado Counties participated Populations tested in Mesa County were invasive Populations tested in Rio Blanco County were native A few others participated but sampling not conclusive
9 HISTORY OF PHRAGMITES IN COLORADO: INITIAL WATCH LIST SURVEY 2013 Presence/Absence County Survey 45 out of 64 counties replied Phragmites received: 5 yes responses 6 maybe responses 1 native response Top 4 Present species: Baby s breath Yellow flag iris Phragmites Swainsonpea
10 HISTORY OF PHRAGMITES IN COLORADO: 2016 EXTENDED WATCH LIST SURVEY Focused on 4 Watch List Species Baby s breath (Gypsophila paniculata) Yellow flag iris (Iris pseudacorus) Swainsonpea (Sphaerophysa salsula) Common reed (Phragmites australis spp. australis) Google Form/Survey Document 93 responses (60 answered questions about Phragmites) County & City weed managers Conservation Districts Non-profits (including the Tamarisk Coalition!) CSU Extension agents Federal & State Agencies Private landowners, community associations, botanic gardens, university staff, utilities, etc.
11 DO YOU KNOW IF THE STANDS OF PHRAGMITES IN YOUR COUNTY ARE NATIVE OR NON-NATIVE?
12 HAVE YOU EVER SUBMITTED A SAMPLE OF YOUR PHRAGMITES FOR IDENTIFICATION? Yes, I have submitted samples to Cornell Univ. for phenotype ID. Yes, I have submitted samples to the Chicago Botanic Garden study. I have not submitted samples, but I will now that I know this is an option. No, I have not submitted samples.
13 WHERE DO YOU THINK PHRAGMITES AUSTRALIS BELONGS ON COLORADO'S NOXIOUS WEED LIST?
14 18 ADDITIONAL COMMENTS ON THE LISTING OF PHRAGMITES Concerns about riparian habitat Some had observed infestation and destruction elsewhere Support for State helping ID and map populations Complex management needed once invasions occur; we should tackle now since populations still small Support for more educational materials and outreach Some wondered if Colorado had enough potential habitat for a statewide Noxious Weed listing
15 CLEAR THAT GENETIC TESTING NEEDED Contract established with CSU in Spring, 2017 CSU established internal genetic testing technique Contract for up to 200 samples Cost slightly less than other options Deliverables include a comprehensive genetic map of native vs. non-native Phragmites Call for sampling 3 s sent over course of summer Sampling Form (1 per site) 2-3 samples per site Supply List & Protocol Sample taken from suspected exotic stand, off HWY 139 in Rio Blanco County
16 SUPPLY LIST AND PROTOCOL Supply list for taking samples: Small Ziploc baggies (2-3 per site; snack or sandwich size) Sharpie to write sample number on Ziploc baggie (county_sitenumber_samplenumber) Moistened paper towels to wrap around samples Field Sample Forms (1 per site; up to 3 samples per form) Clipboard and a pen Mobile phone with EDDMapS West App or GPS device Small cooler to keep the samples from overheating (optional)
17 SUPPLY LIST AND PROTOCOL Preform collections on Monday or Tuesday Find site; collect GPS and/or EDDMapS West data Fill out form Create site name: Jurisdiction_SiteNumber_SampleNumber For example: MesaCounty_101_1 or CityFortCollins_402_2 Write entire name on sample baggies Collect plant samples: Collect 1 full, young leaf from the top of a stem. Wrap the leaf in a moist (not soaking) paper towel. Place the sample in the corresponding Ziploc bag. Collect 2-3 samples per site standard. 2-day delivery or overnight to CSU *If collecting over multiple days, store in fridge to keep fresh
18 FIELD SAMPLE FORM 1 form per site Up to 3 samples per for (only 2 required) Site information GPS Location Habitat Type Approximate size Stand: monoculture or mixed Sample information Number Sampling check Suspected origin: native, introduce, hybrid
19 CSU PHRAGMITES GENETIC TESTING RESULTS
20 CSU PHRAGMITES GENETIC TESTING RESULTS
21 CSU PHRAGMITES GENETIC TESTING RESULTS
22 CSU PHRAGMITES GENETIC TESTING RESULTS
23 NEXT STEPS Finish genetic analysis Decide if and how to list and regulate Phragmites List a subspecies? Regulate and manage by populations? If listed, treatment recommendations will be made via a CDA Factsheet and posted on website Want to make sure we get this right, since there seems to be a lot more native Phragmites than invasive at this time Confirmed native stand Confirmed invasive stand
24 QUESTIONS? Patty York, Early Detection & Rapid Response Specialist Colorado Department of Agriculture, Noxious Weed Program (303)
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