Maximizing HPLC Speed and Resolution with the new 1100 DAD SL

Transcription

1 Welcome to our e-seminar: Maximizing HPLC Speed and Resolution with the new 1100 DAD SL Presenter: Dr. Stefan Schuette

2 Outline Part 1 What do I need to do to maximize HPLC speed? Part 2 What is the performance level I can achieve using the new 1100 DAD SL? Part 3 How does the new DAD SL help to increase data security and traceability? Slide 2

3 Part 1 What do I need to do to maximize HPLC Speed? Slide 3

4 Optimizing Analysis and Cycle Time Speed What makes HPLC fast? 1. Fast Analysis Speed achieved by Short columns with small particles High linear velocity (flow rate F) Short gradient times t_g Elevated Temperature Fast data acquisition 2. Fast Cycle Times (Injection-to-Injection) achieved by Fast and overlapped injections and simultaneous carry over optimization Low software and firmware/instrument overhead time Optional: Parallel, automated column flushing and regeneration Slide 4

5 1100 System Configuration for Ultra-fast LC 1100 Series Binary Pump for precise, high-pressure mixing gradient formation and low delay volume 1100 Series WPS for precise, high-speed injection with lowest delay volume and carry over 1100 Series TCC for precise, peltier-controlled high-temperature LC up to 80C 1100 Series DAD/MWD SL for highest chromatographic resolution by 80Hz data rate Zorbax RRHT 1.8um Columns for highest efficiency at high linear flows Slide 5

6 1100 System Configuration for Ultra-fast LC Recommendations for System Setup and Connecting Capillaries 1100 Binary Pump (G1312A) 1100 WPS (G1367A) 3 µl heat exchanger 4.6mm ID, 1.8um 1100 DAD SL (G1315C) Waste 1100 TCC (G1316A) RRHT Column Replace standard mixer of Binary Pump with 80 µl filter (p/n ) to reduce delay volumne Use low volume, 3ul heat exchanger of TCC G1316A to thermostatt the eluent For 4.6 and 3mm columns use shortest possible 0.17mm ID connecting capillaries Note: In ultra-fast applications the typical flow rate range using 4.6 and 3mm ID columns is 1-5ml/min. At such higher flow rates the larger delay volume of 0.17mm ID capillaries doesn t have a measurable negative impact on chromatographic performance. For 2.1 and 1mm columns use shortest possible 0.12 or 0.1mm ID capillaries Note: In ultra-fast application the typical flow rate range using 2.1 and 1mm ID columns is between 0.1-1ml/min. At these lower flow rates smaller ID connecting capillaries should be used to minimize system delay volume and extra column peak dispersion/band broadening. Inlet tubing of the flow cell should be directly connected to the column. Note: If this is not possible an appropriate low-volume connection should be used (capillary of small ID, i.e. 0.12mm or 0.17mm and ZDV-union). Slide 6

7 How to achieve Maximum Analysis Speed 1. Decrease the column length Advantages: Proportional decrease of analysis time Proportional decrease of solvent consumption Draw back: Drop of Resolution: R ~ SQRT(L) 2. Decrease the particle size Advantage: Increase of Resolution R ~ SQRT(1/d_particle) Draw back: Increase of backpressure 3. Increase the flow rate F Advantage: Decrease of analysis time Isocratic: Analysis Time ~ F Gradient: For Analysis Time ~ F decrease t_g such that F x t_g = const Draw back: Further increase of backpressure 4. Increase the temperature Advantages: Decrease of backpressure Decrease of analysis time Decrease of solvent consumption Slide 7

8 Van Deemter Curves Efficiency of 1.8um Columns HETP (cm/plate) ZORBAX Eclipse XDB-C x 50mm (30mm) 85:15 ACN:Water 1.0µL Octanophenone ml/min 20 C 5.0 µm 260,740 2mL/min 5.0 ml/min 3.5 µm 1.8 µm Interstitial linear velocity (u e - cm/sec) Particle H_min 5µm 9.3µm 3.5µm 1.8µm 6.0µm 3.8µm Note: Efficiency of 1.8µm columns is virtually flow-rate independent. Efficiency gain of 1.8µm versus 5µm columns: 2ml/min 5ml/min Up to 2.1x Resolution Up to 4.4x Speed Slide 8

9 Column Recommendations Zorbax 4.6mm ID RRHT columns for best LC/UV results (speed, resolution, sensitivity) As starting point 4.6 x 50mm is recommended (maximum resolution). If resolution is higher than necessary column length can be reduce for further speed gains. Use SB-C18 for highest performance (allows for Temperatures up to 90C) Leads to minimum 5x gains in analysis speed without loss in resolution compared to 5um columns Same chemistry & selectivity, saves minimum of 80% solvent 4.6mm column can also be used for LC/MS. However, flow splitting might be necessary at F > 1ml/min. Zorbax 2.1mm ID RRHT Columns are recommended for HT LC/MS(/UV) only Disadvantage: Some loss in UV Resolution or Sensitivity compared to 4.6mm columns (compare slide 16) Advantages Low volume UV cell (5ul, 1.7ul, 500nl) for maximum resolution, but reduced S/N Larger volume UV cell (13ul) for maximum S/N, but some extra column broadening Saves >95% solvent compared to 4.6mm ID columns with 5um particles (compare to 80% of 4.6mm) No need for splitting (flow rates < 1ml/min) Slide 9

10 1100 Binary Pumps for Ultra-Fast Gradients High-Pressure Mixing Gradient Pumps for all Needs Binary Pump Binary + Degasser Capillary Pump Nano Pump 0.5 to 5 ml/min 0.05 to 5 ml/min µl/min 10nl/min 4 µl/min 3-8mm id columns 1-4mm id columns 0.1 1mm id columns µm id columns Superior gradient performance and RT precision by high pressure gradient mixing and variable stroke Highest speed and selectivity in fast gradient runs by minimized delay volumes ( ul with 80ul mixer) Lowest detector noise and stability at low flow rates by micro vacuum degasser Highest stability and performance in Cap and Nano LC by electronic flow control Slide 10

11 1100 Series DAD SL for Ultra-fast Detection 80Hz Data Acquisition of up to 8 Signals Up to 100% resolution gain in ultra-fast, quantitative LC 80Hz Full Spectral Data Acquisition for ultra-fast peak purity analysis and spectral conformation even for trace level compounds Improved Diode-Array Front-end Electronics for minimized noise (typical < +/- 6µAU ASTM) New standard flow cell (13ul/10mm) for maximized practical sensitivity by minimized RIsensitivity and dispersion New Electronic Temperature Control for maximized practical sensitivity by reduced baseline wander Builds upon 1100 DAD For compatibility with existing methods, cells and libraries Preserving features like programmable slit and dual lamp design Slide 11

12 DAD SL Method Setup Recommendations Signals: Sample = WL of maximum absorbance BW = NBW of absorbance spectrum Reference = such that WL 0.5 x Ref BW lies outside Absorbance band Reference BW = nm Slit For optimum spectral analysis choose slit such that NBW/Slit > 10 for all compounds For maximum sensitivity slit can be increased to 8 or 16nm Peakwidth For optimum chromatographic resolution choose Peakwidth equal or smaller than FWHM of narrowest peak. For maximum sensitivity choose Peakwidth twice as large as narrowest peak Slide 12

13 DAD SL Method Setup Recommendations Peak Width, Response Time, Data Rate and Sensitivity Don t use for > 0.15 sec peak width > 0.15 sec For 50% peak width of sec > 0.3 sec For 50% peak width of sec > 0.6 sec For 50% peak width of sec > 1.2 sec > 3 sec > 6 sec > 12 sec > 24 sec > 51 sec Peak Width = Peak Width at 50% Peak Height Note and Recommendations: Noise level changes approx. proportional to the square root of the change in response time (data rate). To not give up sensitivity the Peak Width should not be chosen smaller than narrowest peaks To compensate increased noise the slitcanbeincreasedto 8 or16nm. Slide 13

14 DAD SL Flow Cell Recommendations 13µl Standard Flow Cell: For highest sensitivity and linearity High-demanding quantitative work, e.g. analytical method development, QA/QC mm ID Columns Flow Cell Volume/Pathlength 13 µl / 10mm 5 µl / 6mm 1.7 µl / 6mm Signal /Noise Resolution* +++ * Depends on analytical conditions and column dimension µl Micro Flow Cell: For highest selectivity Ultra-fast semi-quantitative work, e.g. Screening Experiments, HT LC/MS/UV 2.1 1mm ID Columns 5µl Semi-micro Flow Cell: Best compromise of sensitivity and selectivity For good quantitative and qualitative results, e.g. Screening, HT LC/MS/UV, Early Formulation Studies 4.6 1mm ID Columns Slide 14

15 1100 Series WPS for High-Speed Injections Delay Volume Reduction and Overlapped Injections Main Pass Bypass Simulteanous Speed and CO optimization by Simple one-valve flow-though design Automatic delay volume reduction (6ul) Overlapped injection Programmable sample flush-out time External needle wash MCO by additional valve switches Slide 15

16 1100 Series WPS for High-Speed Injections Minimizing Carry Over Injection 1 (Switch to mainpass) ADVR (switch to Bypass) or ADVR + MCO (switch BP>MP>BP) Overlapped injection: Next sample drawn and needle moves to wash-port Start external needle wash Needle moves to injection port End Run 1 Injection 2 (Switch to mainpass) t 1 0 Sample flush-out time A: t 1 = 0.65 sec (Flash-out factor 5, no MCO) B: t 1 = 1.30 sec (Flash-out factor 10, MCO) C: t 1 = 2.61 sec (Flash-out factor 20, MCO) t 2 = 29 sec t 2 = 30 sec t 2 = 32 sec t 3 = 35 sec t 3 = 36 sec t 3 = 38 sec 1.2 min 72 sec 1.3 min mau C: CO < 0.003% (LOD) B: CO < 0.5% A: CO < 1% C B A Flow rate 2.3ml/min min How to minimize Carryover when using OI Increase sample flush-out factor Use external needle wash Use minimized carryover option to remove sticky compounds from grooves of injection valve by automated valve switch Slide 16

17 1100 Series WPS for High-Speed Injections Fast Gradients, Short Cycle Times, Low Carry Over, High Precision Fast gradients Short cycle times Minimized carryover High area precision Large linear range WPS µwps Drawn volume = Injected volume 6.2 µl delay volume in overlapped injection mode < 15 sec including inside loop and needle wash < 30 sec including inside loop and needle and external needle wash < 0.003% Beclomethasone < 1% from 1-5µl and < 0.5% from 5-100µl µl (1500µl) without loop change µl (40µl) without loop change Compatibility with small sample amounts Slide 17

18 1100 Valve Solutions for Faster Cycle Times Alternating Column Regeneration with 2 PS/10 PT Valve Shorter cycle times and increased sample throughput for HT and method development labs Saves up to 50% of cycle time by parallel column wash and regeneration Slide 18

19 1100 System Configuration for Ultra-fast LC System with Automated Alternating Column Wash and Regeneration Gradient Equilibration Binary pump 1 (G1312A) Gradient pump 2 (G1312A) 1100 WPS (G1367A) 0.17 mm ID capillary RRHT Column 2PS/10PT Valve G1316A # TCC (G1316A) RRHT Column 4.6 x 50mm, 1.8um 4.6 x 50mm, 1.8um DAD SL (G1315C) Waste Slide 19

20 Part 2 What is the performance level I can achieve using the new 1100 DAD SL? Slide 20

21 What s the Benefit of 80Hz Data Acquisition Rate? Peak Width, Resolution and Peak Capacity in Ultra-Fast LC PW=0.30sec 80Hz versus 20Hz 30% Peak Width + 30% Resolution 80Hz + 40% Peak Capacity PW = 0.33 sec + 70% Apparent Column Efficiency 80Hz versus 10Hz PW=0.42sec 40Hz 55% Peak Width + 90% Resolution + 120% Peak Capacity 20 Hz + 260% Apparent Column Efficiency 0 PW=0.67sec PW=1.24sec 10Hz 5Hz min Sample: Phenone Test Mix Column: Zorbax SB-C18, 4.6x30, 1.8um Gradient: % ACN in 0.3min Temperature: 50, Flow Rate: 5ml Flow cell: 5ul Slit: 8 nm Signal: 245nm, Bandwith: 10nm Reference: 360nm, Bandwidth: 80nm Slide 21

22 Ultra-fast Gradient Analysis of 9 Phenones Increasing Temperature and Flow Rate to push the limits 60 C 4.35mL/min 390bar C mL/min bar C 2.9mL/min 342bar T C Flow ml/min min P bar W 4σ sec Peak Capacity Rs (4,5) Analysis Time/min *Cycle Time/min *Achievable CT with column regeneration Without CR: CT = CT+0.2min min 32 C 2.9mL/min 383bar Sample: 9 Phenones Column: Zorbax SB C x 50mm, 1.8 µm Gradient:: % ACN in 0.65min min 1 min Slide 22

23 Ultra-fast Gradient Analysis of 9 Phenones System Configuration with Automated Alternating Column Regeneration mau ml/min 300 P=300 bar Flow Rate Temp. min Pressure 2.3ml/min 32 C 300 bar 2.6ml/min 32 C 350 bar 2.95ml/min 32 C 390 bar mau ml/min 300 P=350 bar CycleTime Run Time Analysis T. min RT 1.3 min 1.2 min 0.99 min 0.69 min 1.2 min 1.1 min 0.92 min 0.66 min 1.1 min 1.0 min 0.87 min 0.63 min mau ml/min 300 P=390 bar Avg 4σ PW Rs (4,5) % RSD min 0.81 sec 2.62 n.d sec 2.62 n.d sec Comparison of system with and without alternating column regeneration Virtually same analysis time Up to 50% decrease in cycle time by saving column wash and regeneration time Slight decrease of resolution and 4σ peakwdith ( 5% and 10% respectively in this example) Some decrease of precision (RT Precision < 0.2% for system w/o CR and < 1% RSD for system w/ CR) Slide 23

24 Performance Requirements of Ultra-Fast LC Ultra-fast LC using the 1100 DAD SL provides Resolution and Peak Capacity gains of up to 100%. But Can I still fulfill my (regulatory) performance and robustness requirements under ultra-fast LC conditions? Quantification of 0.05% level Impurities with S/N > 10? RT Precision < 0.5%? Area Precision < 1%? Peak Purity Analysis at Trace Levels? Same LOD as in conventional HPLC? Spectral Conformation at Trace Levels? Robustness of method, column and instrument? Slide 24

25 Sensitivity and Linearity in Ultra-Fast LC Can I Simultaneously Quantity Main Compounds and Side Products at 0.05% Level? mau Is the detectors Dynamic Range large enough to accurately quantify Main Compound and Impurities simultaneously? Main Compound (Nimodipin) 2000mAU (100% level) Impurities (Nifedipin) 1mAU (0.05% level) Impurities DMSO min Slide 25

26 Sensitivity and Linearity in Ultra-Fast LC Is the Linear Range Large Enough for my Quantitative Analysis? % Deviation from Linear Value 105.0% 100.0% 95.0% 90.0% 85.0% 80.0% Vis Lamp off Vis Lamp on Absorbance / mau Linearity (Caffeine Sample): Deviation at 2.0AU: 2.0% (Vis Lamp off) 2.5% (Vis Lamp on) 5% Deviation: 2.5 AU (Vis Lamp off) 2.4 AU (Vis Lamp off) Specification: 5% Deviation at 2.0 AU Slide 26

27 Sensitivity and Linearity in Ultra-Fast LC Reproducibility of Main Compounds at 2000mAU (100% Level) mau Gradient: 50 70% B in 0.85min Column: 4.6 x 50, 1.8um Injection: 5ul of 550 µg/ml Nimodipin Flow Rate: 4 ml/min Flow cell: 13ul Data Rate: 80Hz Slit: 8nm Peak Width 0.70 sec Overlay of 10 analyses at 245nm RT Precision: 0.067% RSD Area Precision: 0.13% RSD Typical Requirement: RT Precision: < 0.3% RSD Area Precision: < 0.7% RSD min Slide 27

28 Sensitivity and Linearity in Ultra-Fast LC Is the Noise Low Enough for my Quantitative Analysis? 0 mau 16nm Slit Noise < 4.0 µau mau mau 0 4nm Slit Noise < 7.4 µau nm Slit Noise < 5.4 µau min min min Peak-to-Peak Noise on 13ul Flow Cell 4nm Slit 8nm Slit 16nm Slit 80 Hz Hz Hz Hz Hz Note: 50µAU Noise gives S/N = 20 at 1mAU (0.05% level) Conditions Eluent: Water/ACN = 70/30 Flow rate: 1ml/min 4.6x30mm SB C18, 1.8um Temperature: 20C DAD: 254nm,16nm, Ref 360, 80nm PW: > 0.1min (2.5Hz, 2sec RT) ASTM Noise Specification: 20 µau Peak-to-Peak (+/- 10 µau) Slide 28

29 Sensitivity and Linearity in Ultra-Fast LC Can I quantity Impurities and Side Products at 0.05% Level? mau Nifedipin at trace levels Peak Width = 0.63 sec mAU = 0.1% level S/N = 50 1mAU = 0.05% level S/N = mAU = 0.025% level S/N = 12 min Noise 40 µau Conditions: Column: 4.6 x 50, 1.8um Gradient: 50 70% B in 0.85 min Injection: 5ul Flow Rate: 4 ml/min Flow cell: 13ul Data Rate: 80Hz Slit: 8nm Result: Under ultra-fast LC conditions the DAD SL allows accurate quantitation of impurities and side products at levels smaller than 0.05% of the main compound(s). Slide 29

30 Sensitivity and Linearity in Ultra-Fast LC Reproducibility of Impurities and Side Products at Trace Level mau Overlay of 10 analyses at 245 nm: Column: 4.6 x 50, 1.8um Gradient: 50 70% B in 0.85 min Injection: 5ul Flow Rate: 4 ml/min Flow cell: 13ul Data Rate: 80Hz Slit: 8nm Nifedipin A = 2.5mAU (0.1% level) RT Precision = 0.092% RSD Nifedipin degradation product A = 0.5mAU (0.03% level) RT Precision = 0.123% RSD 1 0 Typical Requirement: RT Precision < 1.0% RSD min Slide 30

31 Sensitivity Limit of Detection in Ultra-fast LC LOD of Anthracene mau pg Anthracene injected in 1ul 4nm slit 80Hz: LOD = 2.30pg 40Hz: LOD = 1.48pg 20Hz, LOD = 1.03pg 10Hz, LOD = 0.67pg min Performance: LOD/pg in Ultra-fast LC Retention Time = 12 sec, Peakwidth = 0.9 sec 80 Hz 40 Hz 20 Hz 10 Hz 5 Hz 4nm Slit nm Slit 1 8nm Slit , nm Slit Compare: Conventional LC on 1100 DAD/MWD B Retention Time = 2 min, Peak width = 6 sec 2.5 Hz 8nm Slit nm Slit 0.53 Slide 31

32 Spectral Analysis in Ultra-Fast LC Can I do Peak Purity and Spectral Conformation at Trace Levels? Reference Spectrum of Nifedipin at 100% level (1800 mau) measured at 80 Hz: mau nm Apex Spectrum of Nifedipin at 0.1% (1.8 mau) measured at 80 Hz: mau Is the spectral quality obtained at trace level under ultra-fast LC conditions and 80Hz spectral sampling rate good enough for peak purity analysis and spectral conformation? nm Slide 32

33 Spectral Analysis in Ultra-Fast LC Can I do 80Hz Peak Purity Analysis at Trace Levels? Norm. 14 DAD1 A, Sig=245,10 Ref=500,80 (N:\BACKUP_MSD\DATA_MRZ\MF_180305_STD_A_ALL\NIMIX_1_2000_1_G_05.D) Fl ma U Overlay of extracted Nifedipin spectra at trace level UV Spectrum of Nifedipin at higher concentration (ca. x180): nm min Result: Nifedipin peak at 0.1% level (1.8mAU) measured with 80Hz spectral rate is pure no other compounds are co-eluting with Nifedipin Slide 33

34 Spectral Analysis in Ultra-Fast LC Can I do 80Hz Spectral Conformation at Trace Levels? Norm Norm Overlay of 80Hz Reference and Apex Spectrum Nifedipin Ref: 1800mAU (100% level) Overlay of 80Hz Reference and Apex Spectrum Nimodipin Ref: 1800mAU (100% level) 1.5 Nifedipin Apex: 1.8mAU (0.1% level) 1.5 Nifedipin Apex: 1.8mAU (0.1% level) Match Factor = Match Factor = nm nm Result: Identification of Nifedipin at 0.1% trace level under fast LC conditions and 80Hz spectral sampling rate Slide 34

35 Robustness in Ultra-Fast LC Are methods, column and instrument robust enough for 24x7 operation? Column 1 injection 4000 P=300b ar Column 1 RT/min injection 2000 injection Column 2 injection 4000 min 0.6 W 1/2 /sec Column 2 RT/min injection W 1/2 /sec injection min Injection Number Stability study on system configuration with automated column regeneration: Stable system and column performance for 8000 injection (4000 injections per column) System suitable for unattended and automated 24x7 operation and reliable over-weekend runs Slide 35

36 Robustness in Ultra-Fast LC Improved Baseline Stability by new Electronic Temperature Control (ETC) Comparison between 1100 DAD SL and 1100 DAD ( B -model) 1100 DAD SL: 254,4 / No REF 1100 DAD SL: 254,4 / 360,100 Ambient Rejection ETC on: DAD B : ~ 100µAU/ C DAD SL: < 30µAU/ C 1100 DAD B: 254,4 / 360,100 ~100µAU/ C 1100 DAD B: 254,4 No REF Temp. RH AH AH Dev. Conditions: Relative humidity = 60%RH = const; Temp = 25 C +/-2 C; 4 x 1h Cycles Note: By keeping RH=const, the absolute humidity is strongly modulated due to temperature variations (worst case condition). C % g/kg % Slide 36

37 Part 3 How does the new DAD SL help to increase data security and traceability? Slide 37

38 New Data Recovery Card* The first LC Detector with Data Never Lost Insurance Data Recovery Card* - DRC All signals, spectra and meta data are buffered on high-capacity, embedded 256MB Compact Flash Card compliant with 21 CFR Part 11. Prevents any data loss in case of communication breakdowns between instrument and PC. Automatic Run Recovery in case of temporary communication failures. Manual Run Recovery in case of permanent communication failures after software, PC, and/or instrument re-boot. *Patent Filed Slide 38

39 New Data Recovery Card The first LC Detector with Data Never Lost Insurance Automated Run Recovery in case of temporary communication failures A) Time Elapsed = 0.3 min Run in Progress B) Time Elapsed = 0.6 min Communication Failure Occurs C) Time Elapsed = 1.9 min Communication is re-established Automatic data transfer DRC > PC No user interaction necessary Slide 39

40 New Data Recovery Card The first LC Detector with Data Never Lost Insurance Manual Run Recovery in case of permanent communication failures Run Recovery dialog pops-up automatically after system reboot. Data are stored on the PC under a pre-configured location. Slide 40

41 DAD SL The Next Level of Data Traceability Proprietary RFID Technology for Flow Cells and UV Lamp Flow Cell Path length Volume Max pressure Date last test passed Product number Serial number Production date UV Lamp Accumulated on-time Actual on-time Number of ignitions Date last test passed Product number Serial number Production date Radio Frequency Identification Tags RFID tags records all relevant data necessary to recall instrument conditions under which a run has been executed. Minimizes the risk of false data interpretation, because measurement conditions are documented. Meta data stored on RFID tags are saved with each raw data file for unambiguous answers to (auditor-) questions like Which type of flow cell was used to generate this chromatogram - what was the path length and volume? Did the accumulated burn-time of the lamp exceed pre-defined limit? Slide 41

42 1100 Series DAD SL and Ultra fast LC More Information DAD SL Product Landing Page Brochure EN Agilent 1100 Series Diode-Array Detector SL Presentation 80Hz Data Acquisition for Ultra-fast LC Article New 1100 Series DAD SL and MWD SL The 1 st for Ultra-fast LC Article New DAD SL Safeguards Your Data Promotion Buy a DAD SL and get a Free Ultra-fast LC Start-up Kit Video New 1100 Series DAD SL and MWD SL DAD SL Product Page RRHT Columns Product Page Technical Note EN Optimization of New High Speed 1100 Series DAD/MWD SL for resolution and sensitivity Technical Note EN (September 2004) Ultra-fast LC using the 1100 and RRHT Columns Notes: Instead of the 500nl flow cell the more cost-effective 1.7ul cell can be used for highest selectivity. Flow cell connecting capillaries: Use 0.12mm ID for 1.7ul and 0.17mm for 13ul cell instead of 0.125mm ID Slide 42

43 Summary Ultra-fast LC Using the 1100 DAD SL 80Hz Data Acquisition for up to 100% resolution gain in ultra-fast LC with peaks < 1sec New Low Noise Electronics, New ETC, New Standard Flow Cell for decreased short-term noise and increased practical sensitivity High Sensitivity, Linearity and Precision to maintain data quality under ultra-fast LC conditions to comply with regulatory requirements to allow for spectral analysis at trace levels High Instrument, Column and Method Stability enables robust 24x7 operation Uncompromised Compatibility with Existing Methods Run conventional methods without compromising data quality Build-in Data Recovery Card provides data never lost insurance RFID Tags for Cells and UV Lamp for unambiguous data traceability Slide 43

44 Wrap-up E-Seminar Questions Thank you for attending today s Agilent e-seminar. Our e-seminar schedule is expanding regularly. Please check our web site frequently at: If you would like to receive regular updates please update your account information under Stay Current with e-notes Slide 44