UV / VIS Spectrophotometer EMCLAB Instruments GmbH

Transcription

1 A Professional Manufacturer UV / VIS Spectrophotometer EMCLAB Instruments GmbH

2 EMCLAB Instruments GmbH Bismarckstrasse Duisburg Germany Phone: Fax: Web: Contact: Mrs. Anke Rehmann, Managing Director 2 info@emc-lab.de

3 EMCLAB Spectrophotometer Selection guide Model Optical System Wavelength Range Bandwidth EMC-11D-V Single Beam nm 4 nm EMC-11-UV Single Beam nm 4 nm EMC-16PC-V Single Beam nm 4 nm EMC-16PC-UV Single Beam nm 4 nm EMC-31PC-UV Single Beam nm 2 nm EMC-32PCS-UV Single Beam Scanning function nm 0.5/1/2/4/5 nm EMC-61PC-UV Double Beam nm 1.8 nm EMC-61PCS-UV Double Beam Scanning function nm 0.5/1/2/4/5 nm EMC-NANO-UV Single Beam 0.2 ~ 2.0 µl nm 4 nm PC means with PC software, Standard central beam height: 15 mm M. Wave Professional PC-Control Software included in: EMC-16PC-V, EMC-16PC-UV UV/VIS Analyst Software included in: EMC-31PC-UV, EMC-31PCS-UV EMC-61PC-UV, EMC-61PCS-UV EMCLAB Spectrophotometers are tested with DAkkS* certified UV/VIS Reference Materials NIST traceable: Photometric Accuracy Wavelength Accuracy Spectral Resolution Stray Light *Accreditation body for the Federal Republic of Germany TRACEABLE info@emc-lab.de 3

4 Visible Spectrophotometer EMC-11D-V EMC-11D-V is the only model of manually setting wavelength, but precise design and high quality components ensures excellent performance. It is widely used in high schools and colleges for general analysis and experiments. Features: Large LCD screen (128*64 Dots) Wavelength can be read out from the screen directly Auto Zero and Blank Parallel port, printed directly Large sample compartment, it can be accommodate mm path length cuvettes with optional holders Pre-aligned design ensures the user can change lamp conveniently The optional PC software M. Wave Basic based on Windows can be expand the applications to Standard Curve & Kinetics High quality silicon photometric diode detector and 1200 lines/mm grating ensure high accuracy and precision Model Wavelength Range Spectral Bandwidth Optical System Wavelength Accuracy Wavelength Repeatability Photometric Accuracy Photometric Range Stray Light Stability Display Photometric Mode Detector Standard Cell Holder Sample compartment Light Source Output Power Requirement Dimensions (LxWxH) Weight EMC-11D-V nm 4 nm Singe Beam, grating 1200 lines/mm ±2 nm 1 nm ±0.5 % T or ±0.003A@1A % T, A, Conc 0.3 % T nm 128*64 LCD T, A, C, F Silicon Photodiode 4-position 10 mm cell changer Standard 10 mm path length cuvette Tungsten lamp USB Port & Parallel Port (Printer) AC 85V~265V 50/60 Hz 489 x 387 x 180 mm 8 kg 4 info@emc-lab.de

5 UV / VIS Spectrophotometer EMC-11-UV EMC-11-UV spectrophotometer is the ideal instrument for education and QC laboratories. Using your standard sample solutions, you can get a standard curve on the large LCD screen. It is widely used in colleges and enterprises for general quantitative analysis and experiments. Features: Large LCD screen (128*64 Dots) Can display total 50 groups of data, 3 groups per screen. Can display standard curve and the curve equation System can also save the test results. Total groups of data and 200 standard curves can be saved; it is convenient for check and reload. Data can be restored after a sudden power cut Auto setting wavelength Tungsten lamp & Deuterium lamp can be turned on/off individually to extend lifetime Pre-aligned design makes it convenient to change lamps Large sample compartment, it can be accommodate mm path length cuvettes with optional holders. A variety of optional accessories are available The optional application software M. Wave Professional provides complete control of the spectrophotometer through the Built-in USB port. You can achieve the following functions: I. Quantitative II. Kinetics III. Wavelength Scan IV. Multi Wavelength V. DNA/Protein Model Wavelength Range Spectral Bandwidth Optical System Wavelength Accuracy Wavelength Repeatability Photometric Accuracy Photometric Range Stray Light Stability Detector Standard Cell Holder Sample compartment Light Source Output Power Requirement Dimensions (LxWxH) Weight EMC-11UV nm 4 nm Singe Beam, grating 1200 lines/mm ±2 nm 1 nm ±0.5 % T or ±0.003A@1A % T, A, Conc. 0.3 % T nm Silicon Photodiode 4-position 10 mm cell changer Standard 10 mm path length cuvette Tungsten & Deuterium lamp USB Port & Parallel Port (Printer) AC 110/220V 50/60 Hz 489 x 387 x 180 mm 14 kg info@emc-lab.de 5

6 UV / VIS Spectrophotometer EMC-16 series EMC-16 series are simple-to-use instruments with advanced performance, its stray light is only 0.05%T. The local stand-alone software provides functions of Photometry, Quantitative Test, Kinetics and System Utilities functions. Features: Large LCD screen (128*64 Dots) System can also save the test results. Total 200 groups of data 200 standard curves can be saved in the RAM. Convenient for check and reload. Data can be restored after a sudden power cut Auto setting wavelength Tungsten lamp & Deuterium lamp can be turned on/off individually to extend lifetime Pre-aligned design makes it convenient to change lamps Large sample compartment, it can be accommodate mm path length cuvettes with optional holders. A variety of optional accessories are available The application software M. Wave Professional provides complete control of the spectrophotometer from a computer through the Built-in USB port. It can expand to the following functions: I. Quantitative, II. Kinetics, III. Wavelength Scan, IV. Multi Wavelength, V. DNA/Protein Functions: 1. Photometry, 2. Quantitative, 3. Kinetics, 4. System Utilities Model EMC-16PC-V EMC-16PC-UV Wavelength Range nm nm Spectral Bandwidth 4 nm Optical System Singe Beam, grating 1200 lines/mm Wavelength Accuracy ±0.5 nm Wavelength Repeatability 0.3 nm Photometric Accuracy ±0.5 % T or ±0.003A@1A Photometric Range % T, A, Conc. Stray Light 0.05 % T@360 nm 0.05 % T@260, 360 nm Stability nm Display Graphic LCD ( dots) Keyboard 22 Membrane keypad Standard Cell Holder 4-position 10 mm cell changer Sample compartment Standard 10 mm path length cuvette Light Source Tungsten lamp Tungsten & Deuterium lamp Output USB Port & Parallel Port (Printer) Power Requirement AC 110/220V 50/60 Hz Dimensions (LxWxH) 470 x 373 x 187 mm Weight 12 kg 14 kg 6 info@emc-lab.de

7 UV / VIS Spectrophotometer EMC-16 series Series Local Control Software Main Menu Move the cursor on the function menu you want, then press ENTER key to go into the corresponding interface 1. Basic Mode Absorbance, Transmittance or Concentration measurements. It can display and save 200 groups of data, 5 groups per screen 2. Quantitative 2.1 Standard Curve A most 9 standard sample can be used to establish a standard curve. The curve and its equation will be displayed on the screen simultaneously. You can measure your unknown concentration solutions by the curve. Total 200 curves can be saved in the RAM 2.2 Coefficient Method If the coefficient k & b in the formula C=k*a + b is known, you can input them directly by the button, and then test your unknown solutions. 3. Kinetics This mode may be used for time course scanning or reaction rate calculation. Abs. VS Time graphs is displayed on the screen in real time. It can most record 1000 data. 4. System Utilities Lamp management, time & data set, obtain dark current, calibrate wavelength, default system, and some other system functions. info@emc-lab.de 7

8 UV / VIS Spectrophotometer EMC-3 series The EMC-3 series are advanced single beam spectrophotometers. They provide excellent performance for measurements in the range of 190 to 1100 nm. The memory is 32K. They are suitable for pharmaceutical, biochemical and clinical lab applications as well as routine applications such as quantitative analysis, kinetics, wavelength scan, multi-wavelength and DNA/Protein analysis. The UV/VIS Analyst software based on Microsoft Windows makes the instruments versatile. The instruments provide excellent performance for measurements. Features: Fixed or variable slits (Bandwidth) Sealed, solvent-resistant tactile keypad with alpha-numeric entry for file names and units Pre-aligned deuterium lamp for easy lamp replacement. The status of the lamp may be monitored Online software upgrading via internet helps to keep it updated Data Download-to-PC software expands the data storage to unlimited The PC model comes standard with Windows based application software UV-VIS Analyst Data can be saved by USB memory device directly Model EMC-31PC-UV EMC-32PCS-UV Wavelength Range nm Spectral Bandwidth 2 nm 0.5/1/2/4/5 nm Optical System Single Beam, grating 1200 lines/mm Wavelength Accuracy ±0.5 nm ±0.3 nm Wavelength Repeatability 0.3 nm 0.2 nm Scan Speed Hi, MED, LOW, MAX nm/min Photometric Accuracy ±0.5 % T or ±0.005A@1A Photometric Range % T, A, Conc. Stray Light 0.05 % T@220, 340 nm Stability nm Display 5 inches LCD (320*240 dots) Baseline Flatness ±0.002A ( nm) Standard Cell Holder 4-position 10 mm cell changer Sample compartment Standard 10 mm path length cuvette Light Source Tungsten & Deuterium lamp (Pre-aligned) USB type A port for USB memory device (Right side) Output USB type B port for optional computer connectivity (Back) Parallel port for printer Power Requirement AC 110/220V 50/60 Hz Dimensions (LxWxH) 491 x 365 x 180 mm 579 x 428 x 198 mm Weight 14 kg 20 kg 8 info@emc-lab.de

9 UV / VIS Spectrophotometer EMC-6 series The EMC-6 series are advanced double beam spectrophotometers. The two detectors measure sample and reference respectively and simultaneous for optimizing measurement accuracy. They provide excellent performance for measurements in the range of 190 to 1100 nm. The memory is 32K. They are suitable for pharmaceutical, biochemical and clinical lab applications as well as routine applications such as quantitative analysis, kinetics, wavelength scan, multiwavelength and DNA/Protein analysis. The UV/VIS Analyst software based on Microsoft Windows makes the instruments versatile. The instruments provide excellent performance for measurements. Features: Fixed or variable slits (Bandwidth) Sealed, solvent-resistant tactile keypad with alpha-numeric entry for file names and units Pre-aligned deuterium lamp for easy lamp replacement. The status of the lamp may be monitored Online software upgrading via internet helps to keep it updated Data Download-to-PC software expands the data storage to unlimited The PC model comes standard with Windows based application software UV-VIS Analyst Data can be saved by USB memory device directly Model EMC-61PC-UV EMC-61PCS-UV Wavelength Range nm Spectral Bandwidth 1.8 nm 0.5/1/2/4/5 nm Optical System Double Beam, grating 1200 lines/mm Wavelength Accuracy ±0.3 nm Wavelength Repeatability 0.2 nm Scan Speed Hi, MED, LOW, MAX nm/min Photometric Accuracy ±0.5 % T or ±0.005A@1A Photometric Range % T, A Stray Light 0.05 % T@220, 340 nm Stability nm Display 5 inches LCD (320*240 dots) Baseline Flatness ±0.001A Standard Cell Holder Standard 10 mm single cell holder (2 pcs.) Sample compartment Standard 10 mm path length cuvette Light Source Tungsten & Deuterium lamp (Pre-aligned) USB type A port for USB memory device (Right side) Output USB type B port for optional computer connectivity (Back) Parallel port for printer Power Requirement AC 110/220V 50/60 Hz Dimensions (LxWxH) 589 x 428 x 200 mm Weight 22 kg info@emc-lab.de 9

10 NANO Spectrophotometer EMC-NANO-UV EMC-NANO-UV the new model for fast, easy, accurate and economic measuring of minimum volumes of 0.2 µl to 2 µl with high quality components ensures excellent performance. It is used in DNA, PCR analyzing within the range from UV to VIS range. Features: Large 5 LCD screen (320*240 Dots) 0.2 µl sample volume for DNA, PCR, protein One step one movement drop close turn and measure Wavelength from nm Pre-aligned design ensures the user can change lamps conveniently Extended output USB A and USB ports for software and printer High quality silicon photometric diode detector and 1200 lines/mm grating ensure high accuracy and precision Model Wavelength Range Spectral Bandwidth Optical System Wavelength Accuracy Wavelength Repeatability Photometric Accuracy Photometric Range Stray Light Stability Detector Multifunctional Cell Unit Scan speed Light Source Output Power Requirement Dimensions (LxWxH) Weight EMC-NANO-UV nm 4 nm Singe Beam, grating 1200 lines/mm ±0.5 nm 0.3 nm ±0.5 % T or ±0.004A@1A % T, A, Conc % T@220, 340 nm nm Silicon Photodiode µl Volume Hi, MED, LOW MAX: 3000nm/min Tungsten & Deuterium lamp 2 USB Port & Parallel Port (Printer) AC 110/220V 50/60 Hz 491 x 365 x 180 mm 14 kg Recommended Pipette Article No. Volume (µl) Increment (µl) EMC info@emc-lab.de

11 UV / VIS Spectrophotometer EMC-3 & EMC-6 series Series Local Control Software All methods are included as built-in standard; this eliminates the need of software. Online software update via internet. The local control software includes functions such as: Photometry, Quantitative, Kinetics, Wavelength Scan, Multi-wavelength Test and System Utilities Standard Curve Up to 10 standard solutions may be used to establish calibration equation curve. There is a choice of four methods for fitting curve through the calibration points: linear fit, Linear fit through zero, square fit and cubic fit. Wavelength Scan The wavelength Scan intervals are 0.1, 0.2, 0.5, 1, 2, 5 nm and High, Medium and Low scan speeds are available. Scan speeds vary from 100 to 3000 nm/min. Wavelengths are scanned fro high to low so that the instrument stand-by at high wavelength. This minimizes the degradation of UV sensitive samples. Precise control of filter and lamp changes means that their effects are not seen on the final can. Post-run manipulation includes re-scaling axes, curve tracking and peak picking. Kinetics This mode may be used for scanning time course or reacting rate calculations. Abs. VS time graphs is displayed on the screen in real time. Wait time and measurement time up to 12 hours may be entered with time intervals of 0.5, 1, 2.5, 10, 30 seconds and 1 min. Post-run manipulation includes re-scaling, curve tracking and selection of the part of the curve required for the rate calculation. Rate is calculated using a linear regression algorithm before multiplying by the entered factor. Multi-Wavelength Up to 10 wavelengths may be entered, allowing the measurement of multiple wavelengths on a series of samples. DNA/Protein Test Concentration and DNA purity are calculated by Absorbance ratios 260 nm/280 nm or 260 nm/230 nm with optional subtracted absorbance at 320 nm DNA concentration = 62.9*A *A 280 or 49.1*A *A 230 Protein concentration = 1552*A *A 280 or 183*A *A 230 Other wavelengths and factors may be entered. info@emc-lab.de 11

12 Spectrophotometer Software M. Wave Professional PC-Control Software for EMC-16 series M. Wave Professional application software is based Microsoft Windows, the instrument can be controlled by PC software through built-in USB communication port, which makes the UV/VIS series with more functions and easy to control. Quantitative Use up to 20 standards to establish standard curve. Three methods for fitting a curve: 1. Linear fit 2. Linear through zero 3. Square fit Kinetics The Kinetics mode may be used for scanning time course or reacting rate calculations. Abs. VS time graphs is displayed. Wavelength Scan Automatically records peaks and valleys. The quantity of the curves stored is unlimited. Post-run manipulation and processing includes 1. Re-scaling axes, curve 2. Smoothing, combination, zooming, overlap 3. 1 st to 4 th derivative Multi-wavelength Test You can set up to 20 wavelengths to measure a sample DNA/Protein Test Optional two formulas: DNA concentration = 62.9*A *A 280 or 49.1*A *A 230 You can also enter other wavelengths and factors to calculate info@emc-lab.de

13 Spectrophotometer Software UV/VIS Analyst Software for EMC-3 series & EMC-6 series The PC application software UV/VIS Analyst takes the features of the stand-alone version plus more powerful data processing, expanded data collecting, and storage capability. It comes standard with EMC- 3 and 6 series. The PC application software offers: 1. Photometric Mode 2. Quantitative Test (Standard curve) 3. Wavelength Scan 4. Kinetics 5. DNA/Protein 6. Multi-Wavelength 7. System Utility Quantitative Test Use up to 20 standards to establish standard curve. Four methods for fitting a curve: 1. Linear fit 2. Linear through zero 3. Square fit 4. Cubic fit Wavelength Scan Automatically records peaks and valleys. The quantity of channels is unlimited. You can simultaneously store as many as desired. Post-run manipulation and processing includes: 1. Re-scaling axes, curve 2. Smoothing, combination, zooming, overlap 3. 1 st to 4 th derivative Kinetics (Abs. VS time) The Kinetics mode may be used for scanning time course or reacting rate calculations. Abs. VS time graphs are displayed on the screen in real time. Wait time, measurement time and time intervals may be entered. Post-run manipulation includes re-scaling, curve tracking and selection of the part of the curve required for the rate calculation. Rate is calculated using a linear regression algorithm before multiplying by the entered factor. info@emc-lab.de 13

14 Spectrophotometer Software DNA/Protein Test Concentration and DNA purity are quickly and easily calculated. Absorbance ratios 260 nm/280 nm with optional subtracted absorbance at 320 nm DNA concentration = 62.9*A *A 280 Protein concentration = 1552*A *A 280 Other wavelengths and factors may be entered. Multi-wavelength Up to 20 wavelengths can be selected and multiple samples can be measured. (auto cell changer is required to run multiple samples automatically) 14 info@emc-lab.de

15 Spectrophotometer Cuvettes Cuvettes Standard Premium Square Glass 10 mm EMC-S0001 EMC-100OS Glass 50 mm EMC-S0002 EMC-105OS Quartz glass 10 mm EMC-S0003 EMC-100QS Quartz glass 50 mm EMC-S0004 EMC-105QS Micro Semi-micro cell glass 1000 µl EMC-S0005 EMC-108OS Semi-micro cell quartz glass 1000 µl n/a EMC-108QS Semi-micro cell quartz glass 1000 µl (black side walls and base) n/a EMC-108QSB Micro cell quartz glass 500 µl n/a EMC-108QSM Micro cell quartz glass 500 µl (black side walls and base) n/a EMC-108QSMB Ultra-micro cell quartz glass 100 µl EMC-S0008 EMC-108QSM15 Flow-through Flow-through cell glass 10 mm EMC-S0009 EMC-175OS Flow-through cell quartz glass 10 mm EMC-S0010 EMC-175QS

16 Spectrophotometer Reference Materials UV/VIS certified reference materials EMC-SET-667UV EMC-SET-666 TRACEABLE EMC-SET-667UV Complete liquid filter set for checking the spectrophotometer acc. to European Pharmacopeia Absorbance Wavelength accuracy Stray light Spectral resolution The set consists of eight liquid filters. They are designed for use with the standard 100 mm cell holder provided with spectrophotometers. The set is supplied in a Pelicase. For identification purpose a number is engraved on each filter. The absorbance values and/or peak position wavelengths of every Filter are quoted in the accompanying calibration certificate. EMC-SET-666 Complete glass filter set for testing Absorbance Wavelength accuracy The set consists of five glass filters and is supplied in a Pelicase Holmium Oxide Filter Neutral Density Glass Filter 0.25 Abs. Neutral Density Glass Filter 0.5 Abs. Neutral Density Glass Filter 1.0 Abs. Empty Filter Mount, Aluminium frame EMC-SET Complete glass filter set for testing Absorbance Wavelength accuracy The set consists of three glass filters and is supplied in a Pelicase Didymium Glass Filter Neutral Density Glass Filter 0.5 Abs. Neutral Density Glass Filter 1.0 Abs. EMC-SET Complete glass filter set for testing Absorbance Wavelength accuracy The set consists of three glass filters and is supplied in a Pelicase Holmium Oxide Filter Neutral Density Glass Filter 0.5 Abs. Neutral Density Glass Filter 1.0 Abs info@emc-lab.de

17 Spectrophotometer Accessories Cell Holder 4-cell holder standard for 10 mm square cuvette EMC cell holder for up to 50 mm square cuvette EMC Cell holder for micro cells, central beam height: 15 mm EMC Single cell holder EMC Single cell holder, water-jacketed EMC cell holder, water-jacketed EMC Peltier Systems Peltier System, Constant-Temperature-System, holder beam height: 15 mm EMC Sipper System, holder beam height: 15 mm EMC Peltier /Sipper System, Constant-Temperature System, holder beam height: 15 mm EMC Lamps Halogen lamp 6V10W (only for EMC-11D-V EMC Halogen lamp 12V20W EMC Halogen lamp 12V20W (only for EMC-6 series) EMC Deuterium lamp for UV EMC Cleaner Cleaning concentrate for cells/cuvettes and optical components, 1 L EMC-S info@emc-lab.de 17

18 Spectrophotometer Calibration Certificate EMC-Spectrophotometers will be delivered with Accuracy Calibration Certificate. Reference Materials for this calibration are DAkkS (German body for accreditation) calibrated and NIST traceable

19 Index Product Description Beam Page EMC-11D-V VIS Single 4 EMC-11-UV UV/VIS Single 5 EMC-16PC-V VIS Single 6 EMC-16PC-UV UV/VIS Single 6 EMC-31PC-UV UV/VIS Single 8 EMC-32PC-UV UV/VIS Single /Scanning 8 EMC-61PC-UV UV/VIS Double 9 EMC-61PCS-UV UV/VIS Double/Scanning 9 EMC-NANO-UV UV/VIS Single 10 EMC-M. Wave Software PC Professional for EMC-16 series 12 EMC-UV/VIS Analyst Software PC for EMC-3 and EMC-6 series 13 EMC-cuvettes Glass and Quartz 10 mm, 50 mm, Standard/Premium 15 EMC-SET/UV/VIS Certified reference materials DAkkS certified/ NIST traceable 16 Spectrophotometer Accessories 17 Calibration Certificate 18 Other types on request Technical changes reserved info@emc-lab.de 19

20 Dealer: EMCLAB Instruments GmbH Bismarckstrasse Duisburg Germany Fon: Fax: Web: