Nicolet Evolution 500. UV-Visible Spectrophotometer
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1 m o l e c u l a r s p e c t r o s c o p y Nicolet Evolution 500 UV-Visible Spectrophotometer Analyze Detect Measure Control
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3 Nicolet Evolution 500 UV-Vis High-Performance, Exceptional Value The Nicolet Evolution 500 system continues our tradition of building high-performance UV-Vis spectrophotometers offering excellent value. The Nicolet Evolution 500 UV-Vis is a system you can trust to deliver the performance needed for your demanding laboratory applications. The double-beam, variablebandwidth, single photomultiplier tube detector (PMT), premium optics and superior quality of system design all contribute to an unsurpassed level of data integrity. The automatic optical alignment of the spectrophotometer occurs at every initialization to guarantee the system is optimized. The system s operation can be evaluated with a Calibration Validation Unit, which verifies that the instrument is working according to factory specifications. In addition, the modular design allows for easy identification and correction of problems. The unique Validation Qualification Calibration (VQC) concept used in the development and manufacture of this instrument provides you with all the evidence needed to prove the quality of data to internal auditors and regulatory agencies. System advantages include: Double-beam optical design provides total system stability High-quality optical components for maximum light throughput Single photomultiplier detector provides excellent sensitivity Variable bandwidth for best resolution and maximum throughput Local Control and PC control options to match various operational needs and laboratory space requirements Multiple security options to address all regulatory needs VQC fully documented, foolproof validation and calibration system
4 Superb Optical Design for Reliable Results The optical design of the Nicolet Evolution 500 system ensures high-quality performance for the analysis of any sample type. The solid-aluminum baseplate guarantees maximum ruggedness and superb stability. Optical surfaces are quartz coated for protection from ozone degradation and other corrosive vapors. Large, 90% reflective mirrors and a single, research-grade Ebert monochromator maximize the energy throughput. In addition, the Nicolet Evolution 500 Interferometric Grating provides exceptional stray light performance with a measurement capability up to 6A. The instrument also uses a unique, intelligent modulator to break the beam rather than divert it with a rotating chopper, thus eliminating chopper wobble. A stepping micrometer, directly driving the Interferometric Grating, guarantees wavelength accuracy and repeatability. The micrometer drive stops to take sample, reference and dark current measurements, removing peak distortions and shifts affecting slewed designs. Monochromator entrance and exit slits are on a circular plate, ensuring wavelength accuracy and bandwidth reproducibility at all bandwidth settings. Motor driven apertures used on older designs often suffer from backlash and wear. Cast-aluminum baseplate High-reflectivity mirrors Quartz-coated optics Ebert-style, stepping monochromator Knife-edge bandpass selection Hatten beam modulator Automatic optical alignment Built-in diagnostics Flexible System Configurations Whether your laboratory requires PC or Local Control operation, the Nicolet Evolution 500 system will meet your needs. A Local Control system is ideal if ease of use is required. A PC-based system, for customers requiring more sophistication and flexibility, is also available. Both options can be combined for ultimate flexibility addressing various skill levels and laboratory needs. Simple and Secure Full-Featured Local Control The Nicolet Evolution 500 Local Control system is designed for laboratories requiring high performance and simple operation in a space-saving design. Local Control offers a full-featured set of UV-Visible software, with no loss of functionality. Results, graphics, calibrations and methods are all displayed on the VGA-quality screen and can be printed on to a variety of printers. Data and methods can be stored in the instrument memory or to a standard floppy disk drive. The proprietary keypad provides excellent resistance to laboratory solvents and chemicals. The reverse printing prevents the characters from wearing off. The integral floppy disk creates the perfect multi-user instrument. Operators can load application methods from their personal disks, run the analysis and save the data to the same disk. The data format can be read by an office PC. Single wavelength Multiple wavelengths Quantification Kinetics Parallel rate Multi-component analysis Ratio and corrected ratio Scanning Advanced results calculation Multiple access control options
5 System Calibration and Validation for Guaranteed Data Quality Thermo Electron is the world leader in UV-Vis spectrophotometer calibration, with a calibration history dating back over 35 years. All Nicolet Evolution 500 spectrophotometers are factory calibrated for wavelength accuracy for the entire UV-Visible range, using fundamental emission lines from mercury lamp and deuterium sources. The unique Calibration Validation Unit (CVU) is offered to test the instrument performance. The CVU is located inside the instrument. The calibrated wavelength and absorbance filters of the CVU are secondary Certified Reference Materials traceable to NPL 1 or NIST 2. The CVU can be set to automatically test the system to factory specifications and generate printed and electronic results. The CVU is only removed when it is returned to Thermo Electron s UKAS 3 and NVLAP 4 certified re-calibration laboratory. Thermo Electron s Validation Qualification Calibration (VQC) concept is designed for easy installation and verification in QA/QC and pharmaceutical laboratories. Validator is a comprehensive set of tools that are used to control the purchase, manufacture, installation and operation of the Nicolet Evolution 500 system. It follows the industry qualification process for system verification in a validated environment. VQC with the Nicolet Evolution 500 UV-Vis meets and surpasses industry performance standards, including European Pharmacopoeia, United States Pharmacopoeia and the American Society of Testing and Materials. Wavelength and absorbance accuracy Stray light measurement Noise and drift measurements NPL and NIST traceability ISO Standard UKAS and NVLAP calibrations Certified Reference Materials Primary NIST filters SQ/DQ/IQ/OQ and PQ documentation 1 National Physical Laboratory, UK 2 National Institute for Standards and Technology, USA 3 United Kingdom Accreditation Service, UK 4 National Voluntary Laboratory Accreditation Program, USA
6 Powerful, PC-controlled System with Multiple Software Options VISIONpro and VISIONsecurity are the principal PC software platforms for use with the Nicolet Evolution 500, with several optional software packages also available. VISIONpro A comprehensive UV-Vis software package with scanning, single-wavelength, multiple-wavelength, quantification and multi-component analysis supplied as standard functions Advanced data manipulation for easy method development and results calculation Advanced spreadsheet results calculation Spectral overlay and manipulation for easy comparison Easy-to-use and customizable interface Complete report flexibility including methods, graphs, data, Microsoft Word files, Excel spreadsheets and graphics Multiple languages Requires Microsoft Windows 98, 2000, NT or XP VISIONlife Life science module for VISIONpro and VISIONsecurity Kinetics for intensity change with time studies Parallel rate measurement for advanced enzyme system measurements Comprehensive manipulation and rate calculation options Enzyme kinetics and equation modeling Additional software packages available for the Nicolet Evolution 500 include: VISION Auto used for automated analysis of multiple samples; VISION Chroma for color measurements; and Dsolve (21 CFR Part 11 compliance package) required for dissolution studies, and dedicated food and wine analysis packages. VISIONsecurity A complete software package with advanced security options and VISIONpro functionality Meets all security requirements of regulated and quality control laboratories Provides tools required to achieve 21 CFR Part 11 compliance Electronic Signatures for data and method traceability Audit trails for data and method manipulation history Requires either Microsoft Windows 2000/NT or 98/2000/NT/XP with an NT or 2000 network Single wavelength Multiple wavelength Quantification Multi-component analysis Scanning Kinetics Parallel rate Advanced enzyme modeling Dissolution Report composer Simple password protection Multiple security levels 21 CFR Part 11 tools Electronic Signatures Autosampler control
7 Accessories for All Application Needs A comprehensive set of accessories designed to support various applications is available for the Nicolet Evolution 500 system. The SuperSipper is the key component used in semi-automated and fully automated system configurations. It is a multiple roller peristaltic pump for processing up to 300 samples per hour. A choice of tubing allows the SuperSipper to deal with aggressive solvents and acids. The 8-cell programmer is engineered for multiple applications. Its fast, precise action makes it ideal for fast parallel kinetics or as a sample changer for automated dissolution testing. A selection of other popular Nicolet Evolution 500 accessories integrate with the spectrophotometer to take advantage of its performance. For a comprehensive accessory list, contact Thermo Electron at the number listed on the back page. a c e b d f g a 8-cell programmer and thermostatted cell holder b Cylindrical cell holder c Variable pathlength cell and filter holder d Peltier 1 e Peltier 178 and single thermostatted cell holder f Combination test tube and cell holder g SuperSipper and autosampler system
8 Nicolet Evolution 500 UV-Vis Spectrophotometer Specifications Optics/Performance Wavelength Range Photometric Range 6.0A Guaranteed (Typical) 190 to 900 nm Photometric Display to 6.0A Detector Bandwidth Best Data Resolution Scan Speeds Monochromator Drive Speed Wavelength Accuracy 1 Single Photomultiplier 0.2, 0.5, 1.0, 1.5, 2.0, 4.0 nm 0.1 nm 3,800 to 1nm/min 4,000 nm/min ± 0.3 nm (± 0.2 nm) Wavelength Repeatability 2 ± 0.1 nm (± 0.03 nm) Photometric Accuracy 3 1A ± 0.002A (± A) 2A ± 0.005A (± A) 3A ± 0.008A (± A) European Pharmacopoeia Photometric Accuracy nm ± 0.010A 257 nm ± 0.010A 313 nm ± 0.010A 350 nm ± 0.010A Photometric Repeatability 5 1A ± 0.001A (0.0005A) 2A ± 0.002A (0.0008A) 3A ± 0.005A (0.0010A) Peak-to-peak Noise 0A at 1.5 nm < A (< A) 1A at 1.5 nm < A (< A) 2A at 1.5 nm < A (< A) Stray Light KCl solution at 200 nm > 2.0A (> 2.5A) NaI solution at 220 nm < 0.02%T (< 0.003%T) NaNO 2 solution at 340 nm < 0.005%T (< 0.001%T) Baseline flatness 6 ± 0.001A (± A) Stability at 340 nm < A/hr (< A/hr) Dimensions (w x d x h), mm 530 x 570 x 230 Dimensions (w x d x h), inches 20.9 x 22.4 x 9 Weight Electrical Supply 32 Kg (70.5 lbs) V, Hz In addition to these offices, Thermo Electron Corporation maintains a network of representative organizations throughout the world. Visit our web site to locate the representative nearest you Australia analyze.au@thermo.com Austria analyze.at@thermo.com Belgium analyze.be@thermo.com Canada analyze.ca@thermo.com China analyze.cn@thermo.com France analyze.fr@thermo.com Germany analyze.de@thermo.com Italy analyze.it@thermo.com Japan analyze.jp@thermo.com Netherlands analyze.nl@thermo.com Nordic analyze.se@thermo.com South Africa analyze.sa@thermo.com Spain analyze.es@thermo.com Switzerland analyze.ch@thermo.com UK analyze.uk@thermo.com USA analyze@thermo.com 1 Wavelength accuracy using holmium and didymium traceable to National Physical Laboratory (NPL) 2 Wavelength repeatability across complete wavelength range using holmium and didymium traceable to NPL (Typical 10 scans of nm D 2 line) 3 Photometric accuracy at 546 nm using neutral density glass filters traceable to NPL 4 European Pharmacopoeia Photometric accuracy test with potassium dichromate 5 Photometric repeatability at 546 nm using neutral density glass filters traceable to NPL 6 Baseline flatness 200 to 800 nm FM ISO Calibration No Accreditation applies to wavelength and Absorbance filters Thermo Electron Corporation. All rights reserved. All product and company names are property of their respective owners. Specifications, terms and pricing are subject to change. Not all products are available in all countries. Please consult your local sales representative for details. P/N /03 BR-S0437
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