Getting Started. Spectra Acquisition Tutorial
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1 Getting Started Spectra Acquisition Tutorial
2 ABB Bomem Inc. All Rights Reserved. This Guide and the accompanying software are copyrighted and all rights are reserved by ABB. This product, including software and documentation, may not, in whole or in part, be copied, photocopied, reproduced, translated, or reduced to any electronic medium or machine-readable form without prior consent, in writing, from ABB Disclaimer This document contains product specifications and performance statements that may be in conflict with other ABB Inc. published literature, such as product flyers and product catalogs. All specifications, product characteristics, and performance statements included in this document are given as suggestive specifications only. In case of conflict between product characteristics given in this document and specifications given in the official ABB Inc. Product Catalogs, the latter take precedence. ABB Inc. reserves the right to make changes to the specifications of all equipment and software, and contents of this document, without obligation to notify any person or organization of such changes. Every effort has been made to insure that the information contained in this document is current and accurate. However, no guarantee is given or implied that the document is error-free or that the information is accurate. ABB Inc. makes no representations or warranties with regard to the product and instructional and reference materials, including, but not limited to, all implied warranties of merchantability and fitness for a particular purpose. ABB Inc. does not warrant, guarantee, or make any representations regarding the use, or the results of the use, of any software or written materials in terms of correctness, accuracy, reliability, currentness, or otherwise. ABB Inc. shall not be liable for errors or omissions contained in its software or manuals, any interruptions of service, loss of business or anticipatory profits and/or for incidental or consequential damages in connection with the furnishing, performance or use of these materials even if ABB Inc. has been advised of the possibility of such damages. All equipment, software, and manuals are sold as is. The entire risk as to the results and performance of the equipment and software is assumed by the user. The software or hardware described in this document is furnished under a license and may be used, copied, or disclosed only in accordance with the terms of such license. Copyright 2011 by ABB. All rights reserved. April 2011 AA Rev. D
3 Table of Contents TABLE OF CONTENTS Section 1 - Spectra Acquisition Spectra Acquisition Tutorial...1 Launching the Horizon MBTM FTIR Software...1 Connecting to the Instrument...2 Record a background or a reference spectrum...2 Set the Instrument for Background Spectrum Acquisition...3 Adjusting the Detector Gain Parameter...6 Collect the Background Spectrum...7 Record a sample spectrum...9 Set the instrument for sample spectrum acquisition...9 Collect a sample spectrum...9 Collect a Kinetic Sample Spectrum...10 i
4 Table of Contents ii
5 Section 1 Spectra Acquisition Spectra Acquisition Tutorial Section 1 Spectra Acquisition Spectra Acquisition Tutorial This guide describes the basic steps to acquire a spectrum with the MB3000 Series Spectrometer using the Horizon MB TM FTIR software. Launching the Horizon MB TM FTIR Software STEP 1 Double click the Horizon MB TM FTIR software desktop icon to launch the software. Figure 1-1. Horizon MB TM FTIR desktop Icon. STEP 2 If the User Identification dialog box appears, 1. Enter the required information. 2. Click OK. AA Rev. D 1
6 Connecting to the Instrument Section 1 Spectra Acquisition Connecting to the Instrument STEP 1 In the Instrument Menu, choose Acquisition. Figure 1-2. Instrument menu Wait for the software to detect available instruments. It may take a few seconds. STEP 2 Select your instrument from the Instrument drop-down menu. Figure 1-3. Instrument drop-down menu Record a background or a reference spectrum Remove any sample from the sampling accessory (cell, sample holder etc.). 2 AA Rev. D
7 Section 1 Spectra Acquisition Set the Instrument for Background Spectrum Acquisition Set the Instrument for Background Spectrum Acquisition STEP 1 Click Live. Figure 1-4. Live spectrum button STEP 2 Set the Acquisition and Detector parameters (with the exception of the Resolution field) as shown in Figure 1-5. Figure 1-5. Acquisition and Detector parameters AA Rev. D 3
8 Set the Instrument for Background Spectrum Acquisition Section 1 Spectra Acquisition STEP 3 Set the Resolution to an appropriated value for the type of application or of samples to be analyzed. Figure 1-6. Resolution drop down menu. 4 AA Rev. D
9 Section 1 Spectra Acquisition Set the Instrument for Background Spectrum Acquisition Examine the Health Monitoring status and the Signal Strength value located in the top right corner of the Acquisition window. The Health Monitoring status should not show any error. Typically, the Signal Strength value should be between 25 and 90%. Optimize the detector gain by following the procedure described in section Adjusting the Detector Gain Parameter on page 6. Figure 1-7. Signal Strength AA Rev. D 5
10 Adjusting the Detector Gain Parameter Section 1 Spectra Acquisition Adjusting the Detector Gain Parameter STEP 1 This procedure serves to maximize the signal-to-noise ratio (SNR) by optimizing the signal treatment inside the detector assembly using the gain value. Increase by one level the Detector Gain value Figure 1-8. Adjusting Detector Gain STEP 2 STEP 3 STEP 4 STEP 5 Observe the Health Monitoring tab title and the Signal Strength value. If the Health Monitoring tab title is not highlighted and all Health Monitoring status are OK proceed with Step 3. If this is the case, the Signal Strength (%) should be below 90%. If Health Monitoring is highlighted in RED and the detector status shows an error, proceed with Step 4. Repeat Steps 1 and 2 until the Health Monitoring tab title is highlighted in RED and the detector status shows an error. Signal Strength value displayed should be higher than 90%. Decrease the Detector Gain value by one level. Click Stop when the Detector Gain parameter is set properly. 6 AA Rev. D
11 Section 1 Spectra Acquisition Collect the Background Spectrum Collect the Background Spectrum STEP 1 Set the Acquisition Parameters (with the exception of the Resolution and Scans field) as shown in Figure 1-9. Figure 1-9. Acquisition Parameter for background collection STEP 2 Set Scans parameter to an appropriate value for the type of application or the samples to be analyzed The Sample Information section contains all the saving parameters for the spectrum to be recorded. By default, the spectrum will be named using a nomenclature that includes the spectrum type and count and date/time stamps. By default, the background spectrum will be saved in C:\Documents and Settings\user name\my Documents\Horizon MB\Reference and the sample spectrum will be saved under the data folder in the Measured Data project. These parameters can be configured and edited if needed. STEP 3 Click Reference to start the acquisition of the background spectrum. AA Rev. D 7
12 Collect the Background Spectrum Section 1 Spectra Acquisition Once the spectral acquisition is finished, the message Measurement finished is displayed in the bottom left corner of the Acquisition window. Figure Measurement finished message 8 AA Rev. D
13 Section 1 Spectra Acquisition Record a sample spectrum Record a sample spectrum Put a sample in the sampling accessory (cell, sample holder etc.). Set the instrument for sample spectrum acquisition STEP 1 Click Live. Refer to Figure 1-4. STEP 2 Examine the Health Monitoring status and the Signal Strength value located in the top right corner of the Acquisition window. The Health Monitoring status should not show any error. Typically, the Signal Strength value should be between 25 and 90%. Optimize the detector gain by following the procedure described in section Adjusting the Detector Gain Parameter on page 6. STEP 3 Once the Detector Gain parameter is set properly, click Stop. Collect a sample spectrum STEP 1 The Resolution parameter must be set to the same value used to record the background spectrum. Set the Scans parameter. Refer to Step 2 of the Adjusting the Detector Gain Parameter on page 6. AA Rev. D 9
14 Collect a Kinetic Sample Spectrum Section 1 Spectra Acquisition STEP 2 Select the desired Acquisition Mode. Figure Acquisition Mode drop down menu STEP 3 STEP 4 The Sample Information section contains all the saving parameters for the spectrum to be recorded. By default, the spectrum will be named using a nomenclature that includes the spectrum type and count and date/time stamps. By default, the background spectrum will be saved in C:\Documents and Settings\user name\my Documents\Horizon MB\Reference and the sample spectrum will be saved under the data folder in the Measured Data project. These parameters can be configured and edited if needed. Click Measure to start the acquisition of the sample spectrum. Once the spectral acquisition is finished, the message Measurement finished is displayed in the bottom left corner of the Acquisition window. Refer to Figure Collect a Kinetic Sample Spectrum The Resolution parameter must be set to the same value used to record the background spectrum. STEP 1 Set the Scans parameter. Refer to Step 2 of the Collect the Background Spectrum on page AA Rev. D
15 Section 1 Spectra Acquisition Collect a Kinetic Sample Spectrum STEP 2 STEP 3 STEP 4 Define the number of consecutive spectra to be recorded by setting the value of the Measurement field. If required, define a time delay before the acquisition of the first spectrum by setting the value of the Initial Delay field. If required, define a time interval between consecutive spectra by setting the value of the Interval field. The time interval between consecutive spectra is defined as the time interval between the beginning of the current spectrum acquisition and the beginning of the next spectrum acquisition (i.e. the time interval between consecutive spectra cannot be smaller than the time required to collect one spectrum). STEP 5 Select the desired Acquisition Mode. Refer to Figure The Sample Information section contains all the saving parameters for the spectrum to be recorded. By default, the spectrum will be named using a nomenclature that includes the spectrum type and count and date/time stamps. By default, the background spectrum will be saved in C:\Documents and Settings\user name\my Documents\Horizon MB\Reference and the sample spectrum will be saved under the data folder in the Measured Data project. These parameters can be configured and edited if needed. STEP 6 Click Measure to start the acquisition of the sample spectrum acquisition. Once the spectral acquisition is finished, the message Measurement finished is displayed in the bottom left corner of the Acquisition window. Refer to Figure AA Rev. D 11
16 Collect a Kinetic Sample Spectrum Section 1 Spectra Acquisition At the end of the acquisition the spectrum is displayed in the main software window, ready to be processed by any Horizon MB TM FTIR tools available according to the purchased software license. Figure Recorded spectrum ready to be processed by any Horizon MB TM FTIR tools available 12 AA Rev. D
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18 AA Rev. D 4/2011 Copyright 2011 by ABB. All Rights Reserved Registered Trademark of ABB. Trademark of ABB. ABB Analytical Measurement 585 Charest Blvd East, suite 300 Québec, Québec G1K 9H4 Canada Phone: North America: 800-HELP ftirservice@us.abb.com Canada: Elsewhere: ftirsupport@ca.abb.com
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