FTIR Microscopy and Imaging
|
|
- Christine Burns
- 6 years ago
- Views:
Transcription
1 FTIR Microscopy and Imaging A world of applications and solutions Dr. Mustafa Kansiz FTIR Imaging and Microscopy Product Manager Agilent Technologies mustafa.kansiz@agilent.com
2 FTIR Spectroscopy - What is it? Fourier Transform InfraRed Spectroscopy is the study of the interaction of infrared light with matter. The vibrations of bonds between atoms in a molecule are excited by IR light leading to absorbances that are specific to chemical structure specific
3 How can FTIR microscopy imaging help me? An FTIR microscope has two essential purposes: 1. To allow users to visually see small (micron) sized samples 2. Collect accurate FTIR spectra from small samples FTIR Imaging takes this to another level by providing spatial and spectral information from an area on your sample FTIR microscopy can collect data in four modes: 1. Single point 2. Single point mapping 3. Linear array mapping 4. 2-D Focal Plane Array (FPA) imaging FTIR Imaging gives spatial (WHERE) and spectral (WHAT) information, and FTIR Chemical Imaging with a IR Focal Plane Array (FPA), gives this simultaneously
4 FTIR Microscope Measurement Modes 1 : Single Point Single or multiple spectra of different zones of a sample 2: Single Point Mapping Automated acquisition of spectra (one by one) defined by a grid. A hundred points can take several hours.
5 FTIR Microscope Measurement Modes: 3: Linear array Mapping Acquisition of spectra by a row (1x16) of detectors. Faster than single point mapping, but still much slower than FPA imaging 4: FPA Imaging With an FPA detector, up to spectra can be recorded simultaneously in a single measurement. Why use FPA chemical imaging? Two reasons: 1. Provides rapid high spatial resolution chemical distribution the where (spatial) and the what (spectral) 2. Allows for the measurement of defects as small as a ~2 microns
6 70 um = What do the Coloured Images Mean? 1724 cm-1 PET ATR Chemical Image O O C 70 um
7 - 70 um = What do the Coloured Images Mean? 1724 cm-1 PET ATR Chemical Image O O C 3295 cm-1 Nylon 70 um H N
8 um = What do the Coloured Images Mean? 1724 cm-1 PET ATR Chemical Image O O C 3295 cm-1 Nylon 2915cm-1 PP 70 um H N H C
9 FTIR Chemical Imaging and its applications Materials/Polymer Polymer laminates (functional layer & adhesive identification) Defect analysis Phase distribution Composites Forensics Car paint layer & structure analysis Trace evidence analysis Pharmaceuticals Ingredient distribution Coating analysis Defect/particle analysis Electronics Defect analysis Biomedical/Biological Research Early disease diagnosis (Cancers) Study of diseases (Alzheimer s, kidney) Plant/fungi tissue studies Live cell chemical imaging (in water) Microbial identification Bone, teeth and cartilage Art Conservation Painting components & layer identification Geology Study of inclusions Food/Cosmetics Study of emulsions, eg cheese, mayonnaise, cream
10 Infrared Microscopy Sampling Techniques Transmission Reflectance Absorption/ Reflectance Micro - ATR Objective Sample Stage θ θ θ θ ATR - IRE Kevley Slide TM Condenser Sample thickness: mm Sample thickness: NA Sample thickness: 5-10 mm Sample thickness: NA
11 Large Sample Microscopy Sampling Techniques Measure small areas on large samples Objective Objective GAO Large Sample Reflectance Large Sample Micro ATR Large Sample Grazing Angle large sample large sample large sample External sample stage External sample stage External sample stage Sample thickness: NA Sample thickness: NA Sample thickness: NA
12 Abs. Large Sample Objective Agilent s patented 1 Large Sample Microscope Objective allows you to measure unlimited sized samples in reflection or ATR single point or imaging mode It s Examples: as simple as: 1. Taking Investigation your sample of new to coating the microscope materials on helmets (military / aerospace / etc.) 2. Placing Large vehicle it against component the 90 degree analysis, objective ex: car door/panel 3. Press collect and get your results! cm -1 Large sample 15x Objective Mirror assembly LS
13 Single point analysis (Single-element detector analysis)
14 Single Point Analysis 1 detector element collects 1 spectrum per scan Desired spatial resolution is attained by fixing aperture size to desired sampling size. This eliminates spectral interference from the surrounding area. Typical best achievable spatial resolution is µm.
15 Transmission IR Analysis of Microtomed 3 layer Laminate (cm -1 ) (cm -1 ) (cm -1 )
16 500 mm 2.0 mm 500 mm Plastic Tubing Defect analysis 4x glass visible objective 15x reflective vis/ir objective ATR 2.5 mm 15x reflective vis/ir objective mm Tube spectrum # ATR Tube spectrum #2 660 mm
17 500 mm 2.0 mm Plastic Tubing Defect analysis 4x glass visible objective 0.10 Glue spectrum # mm 15x reflective vis/ir objective ATR 660 mm
18 Infrared Mapping - Single Point - Linear Arrays
19 Infrared Single Point Mapping One data collect at each point gives one interferogram or spectrum Move the sample with a motorized stage and collect multiple scans Build up a Map of the sample
20 Automatic Single Point Mapping The automated mapping experiment can be used to obtain IR spectra over a sample. Define the field to be mapped. Define spacing of the points to be collected (x and y direction). Define aperture/spatial resolution (the area you are collecting data on an individual scan). You must have visual control of the experiment. You are visually locating areas of interest.
21 Infrared Linear Array Mapping A row of data from each pixel in the array (usually 1 x 16 ) collected simultaneously Move the sample with a motorized stage and collect multiple scans Build up a Map of the sample by stitching linear array scan together Faster than single point mapping, but still much slower than FPA imaging
22 Infrared Mapping in Material Analysis: Multi-layer Paint Sample 1.5 Layer Layer (cm -1 ) (cm -1 ) 0.9 Layer Layer (cm -1 ) (cm -1 ) 1.0 Polyester resin (cm -1 )
23 Infrared Mapping in Material Analysis: Multi-layer Paint Sample Feature image based on absorbance at 3692 cm -1. Abs. at 3692 cm -1
24 Infrared 2-D Focal Plane Array Imaging
25 Principle of Infrared Imaging FPA-FTIR detectors provide the ability to acquire a grid of spectra in the same amount of time that it takes single point detectors to acquire one spectrum Sample is imaged onto a FPA detector (n n pixel array) n 2 spatially resolved spectra are collected simultaneously (e.g., array 256 spectra) One pixel (~5.5µm) is an entire spectrum
26 Achieved Spatial Resolution Summary Pixel Size (obj mag, NA, mode) Achieved Spatial Resolution 3750 cm-1 Achieved Spatial Resolution 2500 cm-1 Single FPA tile FOV (with 128x128FPA) 3.3 um (25x, 0.81NA, std mag) 4.3 um 5.0 um 420x420 um 0.66 um (25x, 0.81NA, high mag) 1.4 um 1.7 um 85x85 um 5.5 um (15x, 0.62NA, std mag) 6.9 um 7.6 um 700x700 um 1.1 um (15x, 0.62NA, high mag) 2.4 um 3.0 um 140x140 um 19 um (4x IR, 0.2NA, std mag) 20.4 um 20.0 um 2400x2400 um 25x obj 0.98um 1.4um Entire 2 x2 (50x50mm) USAF target imaged at 19 um pixel resolution with 4xIR objective in 90 minutes (21x21 tile mosaic with128fpa) USAF target (700x700um) imaged at 5.5 um pixel resolution (normal mag. mode) with 15x objective in 2 minutes Single 128FPA tile USAF target imaged (280x280um) at 1.1 um resolution (high mag mode) with 15x objective in 8 minutes. 2x2 tile mosiac with 128FPA
27 Advancing FTIR Imaging with the Agilent Cary 620 Highest spatial resolution Superior signal-to-noise Largest Field of View Fastest data collection June 12,
28 MARKETS AND APPLICATIONS
29 Polymer Film Laminate FTIR Imaging
30 Sample Preparation Free FTIR Chemical Imaging of Polymer laminates & Films Step 1. Cut out small piece Step 2. Place cut-out piece in micro-vice. Step 3. Cross-section sample with razor Step 4. Place micro-vice (with sample) on microscope stage & touch ATR
31 ATR Contact with sample IR light out IR light in IR light out IR light in micro ATR micro ATR micro-vice Sample 100 micron wide (thick) STEP 5. raise stage to make contact & collect data micro-vice Microscope Stage Sample 100 micron wide (thick) Microscope Stage
32 Live/Real-Time ATR contact monitoring Standard Live ATR direct FPA IR Image without correction No Pressure (before contact) Live ATR direct FPA IR Image with Enhanced Chemical Contrast No Pressure (before contact)
33 Live/Real-Time ATR contact monitoring Standard Live ATR direct FPA IR Image without correction Stage is raised No Pressure (before contact) First Contact Live ATR direct FPA IR Image with Enhanced Chemical Contrast Stage is raised No Pressure (before contact) First Contact
34 Live/Real-Time ATR contact monitoring Standard Live ATR direct FPA IR Image without correction Stage is raised No Pressure (before contact) First Contact Increasing Pressure Live ATR direct FPA IR Image with Enhanced Chemical Contrast Stage is raised No Pressure (before contact) First Contact Increasing Pressure Complete Contact
35 Live/Real-Time ATR contact monitoring Standard Live ATR direct FPA IR Image without correction Stage is raised No Pressure (before contact) First Contact Increasing Pressure Live ATR direct FPA IR Image with Enhanced Chemical Contrast Stage is raised No Pressure (before contact) First Contact Increasing Pressure Complete Contact
36 Live/Real-Time ATR contact monitoring Standard Live ATR direct FPA IR Image without correction Stage is raised No Pressure (before contact) First Contact Increasing Pressure Live ATR direct FPA IR Image with Enhanced Chemical Contrast Stage is raised No Pressure (before contact) First Contact Increasing Pressure Complete Contact
37 Live/Real-Time ATR contact monitoring Standard Live ATR direct FPA IR Image without correction Stage is raised No Pressure (before contact) First Contact Increasing Pressure Live ATR direct FPA IR Image with Enhanced Chemical Contrast Stage is raised No Pressure (before contact) First Contact Increasing Pressure Complete Contact
38 Live/Real-Time ATR contact monitoring Standard Live ATR direct FPA IR Image without correction Stage is raised No Pressure (before contact) First Contact Increasing Pressure Live ATR direct FPA IR Image with Enhanced Chemical Contrast Stage is raised No Pressure (before contact) First Contact Increasing Pressure Complete Contact
39 Sample Presentation A small piece of the sample was cut out and place into a micro-vice sample holder where it was cut flat with a sharp razor The micro-vice is placed in a dedicated insert on the motorised stage and contact is made with the ATR crystal, using Agilent s unique Live ATR contact method, without any need for resin embedding June 12, 2015 Confidentiality Label 39
40 Sausage Packaging (Red): Visible images ATR Imaging Sampling Location 500 um 15x obj. vis image ATR spot 1 ATR spot 3 ATR spot 2 Collection Conditions: 670 um Resolution: Scans (time): Spectral Range: Collection Mode: Pixel Size & FOV: 4 cm-1 32 scans (~30 sec) per spot cm-1 Micro ATR & transmission (5 micron microtomed slices) 1.1 microns/pixel, 70x70 microns
41 Different collection mode comparison 1. Micro ATR (1.1 70x70 micron FOV) 2. Transmission with 15x normal mag (5.5 micron pixel size & 700x700 micron FOV) 3. Transmission with 15x HIGH mag (1.1 micron pixel size & 140x140 micron FOV) 4. Transmission with NEW 25x normal mag (3.3 um pixel size & 420x420 um FOV) 5. Transmission with NEW 25x HIGH mag (0.66 um pixel size & 85x85 um FOV) - June 12, 2015 Confidentiality Label 41
42 70 um 70 um 70 um 70 um 70 um Sausage Packaging ATR Chemical Images (Spot 1) ATR 2850cm Layer 1&3 PE ~46 um thick ATR 1607cm PE (with additive) ~23 um thick Layer 2 70 um x obj. vis image 70 um ATR Chemical RGBY Composite Image Red: PE Yellow: PE (with additive) Green: Polycarboxylic acid ester (possible) Blue: Polyamide ATR 1725 cm um Polycarboxylic acid ester Layer 4 polymer (possibly) ~5 um thick ATR 1202cm Layer 5 polyamide um 70 um June 12, 2015 Confidentiality Label 42
43 cm cm cm cm-1 70 um 2850 cm-1 70 um Sausage Packaging ATR Chemical Images (Spot 2) 15x obj. vis image ATR Chemical Image Layer 3, 7 & 11 PE (with additives) ~6 um thick (centre layer) 70 um 70 um Layer 4 Polycarboxylic acid ester polymer (possibly) ~5 um thick 0.00 Red: PE Green: Polycarboxylic acid polymer (possibly) Blue: Polyamide White: Polypropylene Cyan: EVOH RBGWC composite image Layer 5, 8 & 10 Layer 6 Layer 9 Polyamide ~7um (left) ~2um (centre) ~3um (right) Polypropylene ~20um EVOH ~5um (spectral overlap from Polyamide present) June 12, 2015 Confidentiality Label 43
44 Sausage Packaging ATR Chemical Images (Spot 3) 70 um 70 um 15x high mag. obj. vis image ATR Chemical Image Layer 11 PP (possible with an additive as evident by peak at 1060cm-1) ~37 um thick um 70 um RBC composite image Layer 5, 8 & 10 Polyamide ~2um (left) ~3um (right) Red: PE Blue: Polyamide Cyan: EVOH Layer 9 EVOH ~5um thick (spectral overlap from Polyamide present) 0.00 June 12, 2015 Confidentiality Label 44
45 Sausage Packaging ATR Chemical Images FTIR ATR imaging determined identity & approximate thickness (in microns) PE PE with additive PE Polycarboxylic acid ester polymer (possibly) PA PP PE PA EVOH PA PE 140 um The sample was measured across its entire width with three slightly overlapping ATR measurements, with a total collection time of ~ 1.5 mins. 40x obj. vis image (polarized) As extremely low pressures are applied, there is no sample preparation (via Agilent s live ATR imaging method) with samples being measured as is and no risk of sample surface deformation, which might otherwise make for sequential side-by-side, or slightly overlapping measurements impossible. There was also no evidence of sample carryover between the measurements. With the excellent signal-to-noise data collected, quite a complex sample with at least 11 layers were revealed with spectral library searches assisting in layer identification. 180 um ~11 ~3 ~3 ~24 ~11 ~5 ~7 ~20 ~6 ~5 ~ ~130 um Pixel size: 1.1micron Obj mag: 15x, 0.62NA FOV: 70x70um Total system mag: 36x June 12, 2015 Confidentiality Label 45
46 Sausage Packaging 25x Transmission High mag Chemical Images 140 um - Even in transmission mode, the ultra high NA and very small pixel size has allowed for the resolution of the 3 micron PA layer, hence rivalling the spatial resolution of Ge micro ATR - Most books and papers still talk of ~10um spatial resolution for transmission imaging! - The 25x, 0.81NA is a revolution in objective design 40x obj. vis image (polarized) EVOH, layer um PA, layer 2 PE, layer 1 Pixel size: Obj mag: High mag: FOV: Total system mag: Working distance: 0.66 micron 25x, 0.81NA ON 85x85um 61x 12mm June 12, 2015 Confidentiality Label 46
47 Sausage Packaging 15x Transmission High mag Chemical Images 140 um The ~3 micron PA layers are only just resolved, but the spectral differences between PA and EVOH are now much less, owing to the lower NA, and hence resolving power, of the 15x objective compared to the 25x objective. 40x obj. vis image (polarized) 1 EVOH, layer PA, layer um PE layer 1 Pixel size: Obj mag: High mag: FOV: Total system mag: Working distance: 1.1micron 15x, 0.62NA ON 140x140um 36x 21mm June 12, 2015 Confidentiality Label 47
48 Sausage Packaging 25x Transmission std mag Chemical Images 140 um With the larger pixel size, now at 3.3um, even with the high NA 25x objective, the ~3um PA layers cannot be spatially resolved 40x obj. vis image (polarized) 180 um Pixel size: Obj mag: High mag: FOV: Total system mag: 3.3micron 25x, 0.81NA OFF 420x420um 12x June 12, 2015 Confidentiality Label 48
49 Sausage Packaging 15x Transmission std mag Chemical Images 140 um As the pixel size gets larger, now at 5.5um, layers that are ~5 um now start to blur out 40x obj. vis image (polarized) 180 um Pixel size: 5.5 micron Obj mag: 15x, 0.62NA High mag: OFF FOV: 700x700 um Total system mag: 7.3x June 12, 2015 Confidentiality Label 49
50 Principal Component Noise Reduction (PCNR) 1720 cm-1 - Removes ONLY noise scan - Typical S:N improvement of ~5-10x - Corresponding time saving (for equivalent S:N) is x! scan with PCNR 128 scans Only FTIR imaging system to include PCNR Residual spectrum Agilent Confidential June 12,
51 480 um 70 mm Polymer Film Defects - Visible Images & ATR Imaging Sampling Location 15x obj. vis image cross-section view ATR Chemical Image 0.15 defect, 1402 cm ATR mm 0.06 polymer, 1402 cm um 0.04 Composite (Green/Red) image 0.02 GREEN DEFECT RED - POLYMER 0.00 At initial analysis, it appears that the defect is likely to be an Inorganic material, most probably a carbonate, or a carbonate containing mixture
52 70 mm Image created at 1644 cm um Image created at 2848 cm-1 Micro ATR (FPA) imaging of defects in black rubber sample Vis image IR image ATR 0.05 PE um 640 um Polyamide mm Polyisoprene (natural rubber)
53 85 mm 25x, 0.81 NA Objective in high mag mode for Transmission Polymer bead analysis 40x vis image 2915 cm cm cm-1 (3.4 microns) (6.9 microns) (8.9 microns) 85mm 5 micron polystyrene beads on BaF2 substrate are clearly chemically distinguished. Even at relatively long wavelengths (low wavenumbers), 5 micron beads are clearly resolved Pixel size: Obj mag: High mag: FOV: Total system mag: Working Distance: 0.66 micron 25x, 0.81 NA ON 85x85 um 61x 12 mm Agilent Confidential June 12,
54 350um Exposed green plastic Visible images & ATR Imaging Sampling Location 70 um 15x obj. vis image ATR 70 um 470um
55 Sample Placement & Measurement The large green plastic block was placed directly as-is into the micro-vice holder, which was then placed on the microscope stage, followed by raising of the stage to make contact with the ATR for data collection. Confidentiality Label 55 June 12, 2015
56 70 um 70 um Results - Exposed Green Plastic 40x high mag. obj. vis image ATR Chemical Image cm um 70 um A clear edge containing higher carbonyl content is clearer visible along the expose side edge. Confidentiality Label 56 June 12, 2015
57 57 Pharmaceutical FTIR Imaging
58 480 mm 70 mm Tablet Contamination Location 15x Vis Image 0.20 Row = 29 Col = Active mm 70 mm Row = 12 Col = 10 Lactose Clear and distinct domains from 4 constituents were detected: - Active, compared to provided standard - Lactose, identified from library search - Starch, identified from library search - Cellulose identified from library search Row = 43 Col = Starch Defect (next slide) Row = 51 Col = 28 Cellulose
59 70 mm 480 mm 70 mm Tablet Contamination Location 15x Vis Image Row = 10 Col = 26 Polyester mm 70 mm Clear and distinct domains from 4 constituents were detected: - Contaminant #1 Possibly a polyester - Contaminant #2, - Possibly a polyamide Contaminant Composite (RG), part Row = 30 Col = 28 Polyamide From a single FTIR ATR imaging measurement and without damaging the sample, 4 known constituents and 2 unknown contaminants were imaged in 1 min 70 mm
60 Electronics/Semicon FTIR Imaging
61 Spacer contamination on LCD filter 70 um Total analysis time = 2 mins 350 um um 5 mm 70 um Defects identified as dislodged Spacers No sample prep and no sample damage 0.00
62 LCD Defect Protein image (1647 cm-1) 480 um 70 microns Visible Image 70 microns 640 um Total analysis time = 1 mins Defects identified as protein, most probably flake of dead skin No sample prep and no sample damage Row = 6 Col = 30 IR image at 1647cm
63 350 um 70 um Contaminated Circuit Board FTIR ATR Imaging Visible Image Total analysis time = 2 mins Spectra library search reveals contaminant to be polyetherimide ATR contact damage caused by other vendor 70 um
64 Forensics FTIR Imaging
65 Vehicle paint cross sectional analysis 3696 cm cm Row = 75 Col = Row = 164 Col = cm cm cm cm Row = 94 Col = Row = 24 Col = Row = 90 Col = Row = 149 Col =
66 MIXED POWDER MICRO ATR ANALYSIS CALCIUM CARBONATE 0.8 Row = 37 Col = CaCO3( ) STPP( ) pyrophosphate( ) disodium phosphate(94
67 MIXED POWDER MICRO ATR ANALYSIS DISODIUM DIPHOSPHATE ANHYDRIDE 0.20 Row = 38 Col = STPP( ) disodium phosphate( ) CaCO3( ) pyrophosphate( )
68 MIXED POWDER MICRO ATR ANALYSIS SODIUM TRIPOLYPHOSPHATE 0.2 Row = 61 Col = 56 STPP( ) pyrophosphate( ) disodium phosphate( ) CaCO3( )
69 MIXED POWDER MICRO ATR ANALYSIS SODIUM PYROPHOSPHATE Row = 17 Col = 17 STPP( ) pyrophosphate( ) CaCO3( ) disodium phosphate( )
70 Food/Cosmetics FTIR Imaging
71 500 um Imaging of mayonnaise heterogeneity: measured in transmission on BaF2 slide 1.5 Row = 69 Col = 63 Visible CCD image 1.0 TmpltPk1( ) water( ) starch( ) oil( ) Row = 28 Col = 106 oil( ) water( ) TmpltPk1( ) starch( ) 650 um Measured on FTS7000+UMA600, using a 128x128 FPA. IR measurement area = 700x700 microns Time of measurement = ~ 2min Resolution = 8 cm Row = 70 Col = 66 water( ) starch( TmpltPk1( ) oil( )
72 Biological & Biomedical FTIR Imaging
73 Breast Tissue section CH image ( cm-1) Amide I image 1233 cm-1 image Total IR FOV = 1400x1400 um (2x2 mosaic), pixel size = 5.5um, total data collect time ~ 6 mins, Note: red box, indicates area for single tile high mag collect on next slide
74 Breast Tissue section (high mag mode) 40x obj vis image CH image ( cm-1) Amide I image 1233 cm-1 image Total IR FOV = 280x280 um (2x2 mosaic), pixel size = 1.1um, total data collect time ~ 12 mins, Note: red box, indicates area for single tile high mag collect on next slide
75 280 um 280 um Normal Mag (5.5um) High Mag (1.1um) comparison, CH image Normal Mag (5.5um) High Mag (1.1um) um 280 um The 3 features within this oval shape are each about ~3 um in diameter! June 12, 2015 Confidentiality Label 75
76 Wheat stem cross section FTIR Image See next set of data for med. res. close up 0.8 Protein 1654 cm-1 Row = 26 Col = See following set of data for high. res. close up See next set of data for med. res. close up Lipid 1733 cm-1 Row = 68 Col = See following set of data for high. res. close up See next set of data for med. res. close up 1.0 Carbohydrate cm Row = 57 Col = See following set of data for high. res. close up
77 70 microns 70 microns Visible Image (red box show field of view ATR) 2-D IR image at 1043 cm-1 3-D IR image at 1043 cm Row = 12 Col = 21 IR spectrum from cross-hairs above ATR Imaging has provided for a 5 fold increase in magnification and REAL spatial resolution enhancement. ~5 micron cells wall have been CLEARLY resolved with 10 micron light (~1000 cm-1) and have produced high quality spectra free of artefacts due to diffraction and scattering
78 Live cell FTIR imaging in water! Visible image C-H image Lipid image Protein image Composite (RGB) image Lipid Red C-H Green Protein - Blue 100 microns 100 microns 100 microns 100 microns 100 microns Liquid transmission cell pathlength = 7microns Total measurement time ~ 10mins. Pixel size : 1.1 microns June 12,
79 Summary of Cary FTIR Imaging Highest Spatial Resolution with new 25x, 0.81NA obj. Re-defines, what is possible with actual spatial resolution of <1.5 microns possible in transmission mode! Largest Field of View Measure up to 2.4x2.4mm in a single shot Fastest analysis time Reduce analysis times by >100x, with higher light throughput & PCNR Live FPA Imaging Removes need for complex, time consuming sample prep & allows for delicate analysis June 12,
Advancing FT-IR Imaging Synchrotron IR Imaging in Your Lab. Dr. Mustafa Kansiz FTIR Microscopy & Imaging Product Manager
Advancing FT-IR Imaging Synchrotron IR Imaging in Your Lab Dr. Mustafa Kansiz FTIR Microscopy & Imaging Product Manager How can FTIR microscopy imaging help me? An FTIR microscope has two essential purposes:
More informationAgilent Cary 610/620 FTIR microscopes and imaging systems RESOLUTION FOR EVERY APPLICATION
Agilent Cary 610/620 FTIR microscopes and imaging systems RESOLUTION FOR EVERY APPLICATION AGILENT CARY 610/620 FTIR MICROSCOPES ADVANCING FTIR MICROSCOPY AND IMAGING Agilent s 610/620 FTIR microscopes
More informationMaterial analysis by infrared mapping: A case study using a multilayer
Material analysis by infrared mapping: A case study using a multilayer paint sample Application Note Author Dr. Jonah Kirkwood, Dr. John Wilson and Dr. Mustafa Kansiz Agilent Technologies, Inc. Introduction
More informationDamage-free failure/defect analysis in electronics and semiconductor industries using micro-atr FTIR imaging
Damage-free failure/defect analysis in electronics and semiconductor industries using micro-atr FTIR imaging Application note Electronics and Semiconductor Authors Dr. Mustafa Kansiz and Dr. Kevin Grant
More informationAgilent 8700 LDIR Chemical Imaging System. Bringing Clarity and Unprecedented Speed to Chemical Imaging.
Agilent 8700 LDIR Chemical Imaging System Bringing Clarity and Unprecedented Speed to Chemical Imaging. What if you could save time and achieve better results? The Agilent 8700 Laser Direct Infrared (LDIR)
More informationFT-IR.
FT-IR varian, inc. 610/620-IR ft-ir MICROSCOPY AND IMAGING SoLUTIONS www.varianinc.com VARIAN, INC. Setting the Standard Again When Only the Best Will Do The world leader in molecular spectroscopy innovation
More informationInfrared Microscope AIM-9000 C103-E103B
Infrared Microscope C103-E103B Wide-Field Camera Automatic Recognition Automatic Qualitative High Sensitivity Finally, a wide view on micro sample analysis Surface Micro SHIMADZU AIMs to provide analysis
More informationFTIR microscopy and imaging for failure analysis in electronics manufacturing
FTIR microscopy and imaging for failure analysis in electronics manufacturing Application Note Author Steven M. Barnett, Ellen V. Miseo, and Wayne Jalenak Agilent Technologies, Inc. Introduction The electronics
More informationCharacteristics and Applications of Imaging Microscope FT-IR. Teo Wei Boon PerkinElmer Singapore
Characteristics and Applications of Imaging Microscope FT-IR Teo Wei Boon PerkinElmer Singapore Infrared (IR) Spectroscopy. IR Spectroscopy measures the radiation absorbed by materials due to molecular
More informationDevelopment and Applications of a Sample Compartment FTIR Microscope
Application Note Development and Applications of a Sample Since the early to mid-1940 s, scientists using infrared spectroscopy have been trying to obtain spectral data from ever smaller samples. Starting
More informationInfrared Microscope. Dedicated AIMsolution Software. Hisato Fukuda. 1. Introduction. 2. Automatic Contaminant Recognition Function
C103-E120 Vol. 28 Infrared Microscope Dedicated AIMsolution Software ------- 02 Infrared Microscope Using Imaging Analysis ------- 05 EDXIR-Analysis EDX-FTIR Contaminant Finder/Material Inspector -------
More informationFT-IR IMAGING THAT'S CLEARLY MEASURABLY AMAZING. Spotlight 400 FT-IR and 400N FT-NIR Imaging Systems
FT-IR IMAGING THAT'S CLEARLY MEASURABLY AMAZING Spotlight 400 FT-IR and 400N FT-NIR Imaging Systems YOUR CHALLENGES COME IN ALL SHAPES AND SIZES ONE SYSTEM CAN HANDLE THEM ALL It s been called the most
More informationVol. Validation of FTIR Systems AIM-9000 Infrared Microscope Infrared Microscope. Convenience of a Wide-View Camera
C103-E119 Vol. 27 Validation of FTIR Systems ------- 02 Infrared Microscope Convenience of a Wide-View Camera ------- 06 AIM-9000 Infrared Microscope ------- 10 Validation of FTIR Systems Spectroscopy
More informationFastest high definition Raman imaging. Fastest Laser Raman Microscope RAMAN
Fastest high definition Raman imaging Fastest Laser Raman Microscope RAMAN - 11 www.nanophoton.jp Observation A New Generation in Raman Observation RAMAN-11 developed by Nanophoton was newly created by
More informationINFRARED ANALYSIS OF SINGLE AND MULTILAYER FILMS IN THE PRODUCTION AREA
INFRARED ANALYSIS OF SINGLE AND MULTILAYER FILMS IN THE PRODUCTION AREA Sandy Rintoul Wilks Enterprise, Inc. South Norwalk, CT Scott Cobranchi Sealed Air Corporation Duncan, SC Nina Tani Sealed Air Corporation
More informationApplications of Optics
Nicholas J. Giordano www.cengage.com/physics/giordano Chapter 26 Applications of Optics Marilyn Akins, PhD Broome Community College Applications of Optics Many devices are based on the principles of optics
More informationObservational Astronomy
Observational Astronomy Instruments The telescope- instruments combination forms a tightly coupled system: Telescope = collecting photons and forming an image Instruments = registering and analyzing the
More informationSpectrum 400. FT-IR and FT-NIR Spectrometer. There is only one answer.
Spectrum 400 FT-IR and FT-NIR Spectrometer There is only one answer. The latest innovation in PerkinElmer s long history of IR technology leadership For over 60 years, PerkinElmer has been the world leader
More informationFast Laser Raman Microscope RAMAN
Fast Laser Raman Microscope RAMAN - 11 www.nanophoton.jp Fast Raman Imaging A New Generation of Raman Microscope RAMAN-11 developed by Nanophoton was created by combining confocal laser microscope technology
More informationFast Laser Raman Microscope RAMAN
Fast Laser Raman Microscope RAMAN - 11 www.nanophoton.jp Fast Raman Imaging A New Generation of Raman Microscope RAMAN-11 developed by Nanophoton was created by combining confocal laser microscope technology
More informationC103-E056C. Shimadzu Infrared Microscope
C103-E056C Shimadzu Infrared Microscope Complete Control with AIMView Right on Target... This intelligent infrared microscope provides convenient control of stage movement, aperture setting and focusing,
More informationSpotlight 150 and 200 FT-IR Microscopy Systems
S P E C I F I C A T I O N S Spotlight 150 and 200 FT-IR Microscopy Systems FT-IR Microscopy Spotlight 200 with Frontier FT-IR Spectrometer Introduction PerkinElmer Spotlight FT-IR Microscopy Systems are
More informationΕισαγωγική στην Οπτική Απεικόνιση
Εισαγωγική στην Οπτική Απεικόνιση Δημήτριος Τζεράνης, Ph.D. Εμβιομηχανική και Βιοϊατρική Τεχνολογία Τμήμα Μηχανολόγων Μηχανικών Ε.Μ.Π. Χειμερινό Εξάμηνο 2015 Light: A type of EM Radiation EM radiation:
More informationMore Detail. Faster. Easier. The results will inspire you. Spotlight 400
More Detail. Faster. Easier. The results will inspire you. Spotlight 400 FT-IR and 400N FT-NIR Imaging Systems Raising the Level of Lab Productivity to an Art From SPOTLIGHT 400 FT-IR AND 400N FT-NIR IMAGING
More informationThe FTNIR Myths... Misinformation or Truth
The FTNIR Myths... Misinformation or Truth Recently we have heard from potential customers that they have been told that FTNIR instruments are inferior to dispersive or monochromator based NIR instruments.
More informationApproachable Raman Solutions The Shortest Path from Problem to Answer
Approachable Raman Solutions The Shortest Path from Problem to Answer Michael S. Bradley The world leader in serving science Thermo Scientific Raman Spectroscopy: Discover. Solve. Assure. Raman Spectroscopy
More informationWHITE PAPER MINIATURIZED HYPERSPECTRAL CAMERA FOR THE INFRARED MOLECULAR FINGERPRINT REGION
WHITE PAPER MINIATURIZED HYPERSPECTRAL CAMERA FOR THE INFRARED MOLECULAR FINGERPRINT REGION Denis Dufour, David Béland, Hélène Spisser, Loïc Le Noc, Francis Picard, Patrice Topart January 2018 Low-cost
More informationAgilent 4300 Handheld FTIR Spectrometer AT-SITE. IMMEDIATE RESULTS. TRUE NON-DESTRUCTIVE ANALYSIS.
Agilent 4300 Handheld FTIR Spectrometer AT-SITE. IMMEDIATE RESULTS. TRUE NON-DESTRUCTIVE ANALYSIS. BRING THE POWER OF FTIR SPECTROSCOPY OUT OF THE LAB AND TO THE SAMPLE From improving composite bonding
More informationBringing Answers to the Surface
3D Bringing Answers to the Surface 1 Expanding the Boundaries of Laser Microscopy Measurements and images you can count on. Every time. LEXT OLS4100 Widely used in quality control, research, and development
More informationNikon Instruments Europe
Nikon Instruments Europe Recommendations for N-SIM sample preparation and image reconstruction Dear customer, We hope you find the following guidelines useful in order to get the best performance out of
More informationResolution. Diffraction from apertures limits resolution. Rayleigh criterion θ Rayleigh = 1.22 λ/d 1 peak at 2 nd minimum. θ f D
Microscopy Outline 1. Resolution and Simple Optical Microscope 2. Contrast enhancement: Dark field, Fluorescence (Chelsea & Peter), Phase Contrast, DIC 3. Newer Methods: Scanning Tunneling microscopy (STM),
More informationThermo Scientific Nicolet in10 FT-IR Microscope. Infrared microscopy. with confidence. intuitive innovative integrated
Thermo Scientific Nicolet in10 FT-IR Microscope Infrared microscopy with confidence intuitive innovative integrated Thermo Scientific Nicolet in10 FT-IR Microscope The Infrared Microscope that Delivers
More informationHoriba Jobin-Yvon LabRam Raman Confocal Microscope (GERB 120)
Horiba Jobin-Yvon LabRam Raman Confocal Microscope (GERB 120) Please contact Dr. Amanda Henkes for training requests and assistance: 979-862-5959, amandahenkes@tamu.edu Hardware LN 2 FTIR FTIR camera 1
More informationNicolet Continuμm Infrared Microscope
m o l e c u l a r s p e c t r o s c o p y Nicolet Continuμm Infrared Microscope Infrared Microscopy Excellence Crystal-clear Visible Microscopy Superior Purity Infrared Spectroscopy New Dimensions in Imaging
More informationTerahertz Spectroscopic/ Imaging Analysis Systems
Terahertz Spectroscopic/ Series Non-Destructive Analysis of Pharmaceuticals, Chemicals, Communication Materials, etc. Compact, High-Speed Terahertz Spectroscopic/ High-speed measurement functionality Compact,
More informationApplications of Steady-state Multichannel Spectroscopy in the Visible and NIR Spectral Region
Feature Article JY Division I nformation Optical Spectroscopy Applications of Steady-state Multichannel Spectroscopy in the Visible and NIR Spectral Region Raymond Pini, Salvatore Atzeni Abstract Multichannel
More informationChemical Imaging. Whiskbroom Imaging. Staring Imaging. Pushbroom Imaging. Whiskbroom. Staring. Pushbroom
Chemical Imaging Whiskbroom Chemical Imaging (CI) combines different technologies like optical microscopy, digital imaging and molecular spectroscopy in combination with multivariate data analysis methods.
More informationDynamic Phase-Shifting Microscopy Tracks Living Cells
from photonics.com: 04/01/2012 http://www.photonics.com/article.aspx?aid=50654 Dynamic Phase-Shifting Microscopy Tracks Living Cells Dr. Katherine Creath, Goldie Goldstein and Mike Zecchino, 4D Technology
More informationMICROSCOPE LAB. Resolving Power How well specimen detail is preserved during the magnifying process.
AP BIOLOGY Cells ACTIVITY #2 MICROSCOPE LAB OBJECTIVES 1. Demonstrate proper care and use of a compound microscope. 2. Identify the parts of the microscope and describe the function of each part. 3. Compare
More informationIdentify extraneous chemicals that contributed towards the failure of actuating mechanism in inner vial
Identify extraneous chemicals that contributed towards the failure of actuating mechanism in inner vial By Vishu Shah Consultek 460-D West Lambert Road Brea, CA 92821 1 Identify extraneous chemicals that
More informationOriel MS260i TM 1/4 m Imaging Spectrograph
Oriel MS260i TM 1/4 m Imaging Spectrograph MS260i Spectrograph with 3 Track Fiber on input and InstaSpec CCD on output. The MS260i 1 4 m Imaging Spectrographs are economical, fully automated, multi-grating
More informationAn NDVI image provides critical crop information that is not visible in an RGB or NIR image of the same scene. For example, plants may appear green
Normalized Difference Vegetation Index (NDVI) Spectral Band calculation that uses the visible (RGB) and near-infrared (NIR) bands of the electromagnetic spectrum NDVI= + An NDVI image provides critical
More informationVision. The eye. Image formation. Eye defects & corrective lenses. Visual acuity. Colour vision. Lecture 3.5
Lecture 3.5 Vision The eye Image formation Eye defects & corrective lenses Visual acuity Colour vision Vision http://www.wired.com/wiredscience/2009/04/schizoillusion/ Perception of light--- eye-brain
More informationMS260i 1/4 M IMAGING SPECTROGRAPHS
MS260i 1/4 M IMAGING SPECTROGRAPHS ENTRANCE EXIT MS260i Spectrograph with 3 Track Fiber on input and InstaSpec IV CCD on output. Fig. 1 OPTICAL CONFIGURATION High resolution Up to three gratings, with
More informationThermo Scientific Nicolet in10 FT-IR Microscope
m o l e c u l a r s p e c t r o s c o p y Thermo Scientific Nicolet in10 FT-IR Microscope Infrared Microscopy with Confidence intuitive innovative integrated Part of Thermo Fisher Scientific Thermo Scientific
More informationPHYSICS. Chapter 35 Lecture FOR SCIENTISTS AND ENGINEERS A STRATEGIC APPROACH 4/E RANDALL D. KNIGHT
PHYSICS FOR SCIENTISTS AND ENGINEERS A STRATEGIC APPROACH 4/E Chapter 35 Lecture RANDALL D. KNIGHT Chapter 35 Optical Instruments IN THIS CHAPTER, you will learn about some common optical instruments and
More informationBruker Optics Inc. Bruker Hong Kong Ltd. Billerica, MA USA Phone +1 (978) Fax +1 (978)
Bruker Optics is ISO 9001 certified. Laser class 1 Technologies used are protected by one or more of the following patents: US 5309217; DE 4212143; DE 104025448; DE 19940981; US 5923422; DE 19704598 www.brukeroptics.com
More informationATR FT-IR Imaging of Human Hair Cross-Section
a p p l i c a t i o n N O T E TR FT-IR Imaging of Human Hair Cross-Section FT-IR Imaging Introduction Human hair is typically 50-100 μ in diameter, previous transmission and TR studies of its cross section
More informationAdvancing EDS Analysis in the SEM Quantitative XRF. International Microscopy Congress, September 5 th, Outline
Advancing EDS Analysis in the SEM with in-situ Quantitative XRF Brian J. Cross (1) & Kenny C. Witherspoon (2) 1) CrossRoads Scientific, El Granada, CA 94018, USA 2) ixrf Systems, Inc., Houston, TX 77059,
More informationImproving the Collection Efficiency of Raman Scattering
PERFORMANCE Unparalleled signal-to-noise ratio with diffraction-limited spectral and imaging resolution Deep-cooled CCD with excelon sensor technology Aberration-free optical design for uniform high resolution
More informationHuman Retina. Sharp Spot: Fovea Blind Spot: Optic Nerve
I am Watching YOU!! Human Retina Sharp Spot: Fovea Blind Spot: Optic Nerve Human Vision Optical Antennae: Rods & Cones Rods: Intensity Cones: Color Energy of Light 6 10 ev 10 ev 4 1 2eV 40eV KeV MeV Energy
More informationNanonics Systems are the Only SPMs that Allow for On-line Integration with Standard MicroRaman Geometries
Nanonics Systems are the Only SPMs that Allow for On-line Integration with Standard MicroRaman Geometries 2002 Photonics Circle of Excellence Award PLC Ltd, England, a premier provider of Raman microspectral
More informationAdd CLUE to your SEM. High-efficiency CL signal-collection. Designed for your SEM and application. Maintains original SEM functionality
Add CLUE to your SEM Designed for your SEM and application The CLUE family offers dedicated CL systems for imaging and spectroscopic analysis suitable for most SEMs. In addition, when combined with other
More informationM4 TORNADO PLUS. Innovation with Integrity. Super Light Element Micro-XRF Spectrometer. Micro-XRF
M4 TORNADO PLUS Super Light Element Micro-XRF Spectrometer Innovation with Integrity Micro-XRF M4 TORNADO PLUS - A New Era in Micro-XRF M4 TORNADO PLUS is the world's first Micro-XRF spectrometer that
More informationTRAINING MANUAL. Multiphoton Microscopy LSM 510 META-NLO
TRAINING MANUAL Multiphoton Microscopy LSM 510 META-NLO September 2010 Multiphoton Microscopy Training Manual Multiphoton microscopy is only available on the LSM 510 META-NLO system. This system is equipped
More informationMeasurement of Microscopic Three-dimensional Profiles with High Accuracy and Simple Operation
238 Hitachi Review Vol. 65 (2016), No. 7 Featured Articles Measurement of Microscopic Three-dimensional Profiles with High Accuracy and Simple Operation AFM5500M Scanning Probe Microscope Satoshi Hasumura
More informationmicroscopy A great online resource Molecular Expressions, a Microscope Primer Partha Roy
Fundamentals of optical microscopy A great online resource Molecular Expressions, a Microscope Primer http://micro.magnet.fsu.edu/primer/index.html Partha Roy 1 Why microscopy Topics Functions of a microscope
More informationINVESTIGATION OF IMPROVED LABEL CUTTING BY CO 2 LASERS WITH WAVELENGTH OPTIMIZATION Paper #2004
INVESTIGATION OF IMPROVED LABEL CUTTING BY CO 2 LASERS WITH WAVELENGTH OPTIMIZATION Paper #2004 Justin Conroy 1, 1 Applications Lab, Synrad Inc. Mukilteo, WA, 98275, USA Abstract The digital printing revolution
More informationMicroscopy. Matti Hotokka Department of Physical Chemistry Åbo Akademi University
Microscopy Matti Hotokka Department of Physical Chemistry Åbo Akademi University What s coming Anatomy of a microscope Modes of illumination Practicalities Special applications Basic microscope Ocular
More informationReprint (R37) DLP Products DMD-Based Hyperspectral Imager Makes Surgery Easier
Reprint (R37) DLP Products DMD-Based Hyperspectral Imager Makes Surgery Easier Reprinted with permission by Dr. Karel J. Zuzak University of Texas/Arlington October 2008 Gooch & Housego 4632 36 th Street,
More informationNear-Infrared Spectral Imaging for the Visualization of Moisture Distribution inside Soybean Seeds
1/ 31 Near-Infrared Spectral Imaging for the Visualization of Moisture Distribution inside Soybean Seeds M. Tsuta 1, Y. Ogawa 2, Y. Sagara 3, G. Do 4 and J. Sugiyama 1 1 National Food Research Institute,
More informationChapter 2 The Study of Microbial Structure: Microscopy and Specimen Preparation
Chapter 2 The Study of Microbial Structure: Microscopy and Specimen Preparation 1 Lenses and the Bending of Light light is refracted (bent) when passing from one medium to another refractive index a measure
More informationLUMOS. Innovation with Integrity. FTIR Microscopy made easy FTIR
LUMOS FTIR Microscopy made easy Innovation with Integrity FTIR LUMOS: A Clear Vision LUMOS is a stand-alone FTIR microscope with full automation. It is designed to combine best performance for visible
More informationObserving Microorganisms through a Microscope LIGHT MICROSCOPY: This type of microscope uses visible light to observe specimens. Compound Light Micros
PHARMACEUTICAL MICROBIOLOGY JIGAR SHAH INSTITUTE OF PHARMACY NIRMA UNIVERSITY Observing Microorganisms through a Microscope LIGHT MICROSCOPY: This type of microscope uses visible light to observe specimens.
More information2014 HTD-E with options
with options The HT7700 : a user-friendly, ergonomic digital TEM with options User-Friendly r end Design Ambient light operation. Multiple automated functions for alignment, focus and stigmation as standard
More informationPhotoacoustic Imaging of Blood Vessels in Tissue
of Blood Vessels in Tissue F.F.M. de Mul (University of Twente, Enschede, the Netherlands) FdM [µm] Imaging methods for hidden structures in turbid media (tissue) OCT/ OPS (C)M TOF / FM NIR green C(M)
More informationTissue Preparation ORGANISM IMAGE TISSUE PREPARATION. 1) Fixation: halts cell metabolism, preserves cell/tissue structure
Lab starts this week! ANNOUNCEMENTS - Tuesday or Wednesday 1:25 ISB 264 - Read Lab 1: Microscopy and Imaging (see Web Page) - Getting started on Lab Group project - Organ for investigation - Lab project
More informationInstallation and User Guide. FlexIR TM NIR Fiber Optic Accessory
Installation and User Guide FlexIR TM NIR Fiber Optic Accessory The information in this publication is provided for reference only. All information contained in this publication is believed to be correct
More informationApplication Note AN M102. FTIR Microscopic Identification of Fibers
Application Note AN M102 FTIR Microscopic Identification of Fibers Introduction The identification of fibers is of hih importance in forensic science as it can yield trace evidence in a criminal case.
More informationPhilippa Eshun. Lincoln Leadership Academy Charter School (Allentown, PA)
Outreach Program: Plastics are all around us. Support: NSF Polymer Program NSF-1308617 (PI: Chang Ryu)/RPI Polymer Center Understanding FTIR and Polymer Plastics Philippa Eshun Lincoln Leadership Academy
More informationSFR 406 Spring 2015 Lecture 7 Notes Film Types and Filters
SFR 406 Spring 2015 Lecture 7 Notes Film Types and Filters 1. Film Resolution Introduction Resolution relates to the smallest size features that can be detected on the film. The resolving power is a related
More informationFLUORESCENCE MICROSCOPY. Matyas Molnar and Dirk Pacholsky
FLUORESCENCE MICROSCOPY Matyas Molnar and Dirk Pacholsky 1 The human eye perceives app. 400-700 nm; best at around 500 nm (green) Has a general resolution down to150-300 μm (human hair: 40-250 μm) We need
More informationLeading in Desktop SEM Imaging and Analysis
Leading in Desktop SEM Imaging and Analysis Fast. Outstanding. Reliable SEM imaging and analysis. The Phenom: World s Fastest Scanning Electron Microscope With its market-leading Phenom desktop Scanning
More informationNIR SPECTROSCOPY Instruments
What is needed to construct a NIR instrument? NIR SPECTROSCOPY Instruments Umeå 2006-04-10 Bo Karlberg light source dispersive unit (monochromator) detector (Fibres) (bsorbance/reflectance-standard) The
More informationSome of the important topics needed to be addressed in a successful lens design project (R.R. Shannon: The Art and Science of Optical Design)
Lens design Some of the important topics needed to be addressed in a successful lens design project (R.R. Shannon: The Art and Science of Optical Design) Focal length (f) Field angle or field size F/number
More informationSpectral phase shaping for high resolution CARS spectroscopy around 3000 cm 1
Spectral phase shaping for high resolution CARS spectroscopy around 3 cm A.C.W. van Rhijn, S. Postma, J.P. Korterik, J.L. Herek, and H.L. Offerhaus Mesa + Research Institute for Nanotechnology, University
More informationRaman Imaging: Unlocking Solid Dosage Form Evaluation. Robert Heintz, Ph.D. Senior Applications Specialist
Raman Imaging: Unlocking Solid Dosage Form Evaluation Robert Heintz, Ph.D. Senior Applications Specialist Agenda Brief overview of Raman spectroscopy & Raman imaging Introducing the Thermo Scientific DXR
More informationWITec Alpha 300R Quick Operation Summary October 2018
WITec Alpha 300R Quick Operation Summary October 2018 This document is frequently updated if you feel information should be added, please indicate that to the facility manager (currently Philip Carubia,
More informationLight Microscopy. Upon completion of this lecture, the student should be able to:
Light Light microscopy is based on the interaction of light and tissue components and can be used to study tissue features. Upon completion of this lecture, the student should be able to: 1- Explain the
More information3D light microscopy techniques
3D light microscopy techniques The image of a point is a 3D feature In-focus image Out-of-focus image The image of a point is not a point Point Spread Function (PSF) 1D imaging 1 1 2! NA = 0.5! NA 2D imaging
More informationFast Raman Spectral Imaging Using Chirped Femtosecond Lasers
Fast Raman Spectral Imaging Using Chirped Femtosecond Lasers Dan Fu 1, Gary Holtom 1, Christian Freudiger 1, Xu Zhang 2, Xiaoliang Sunney Xie 1 1. Department of Chemistry and Chemical Biology, Harvard
More informationTRACE EVIDENCE ANALYSIS UV-VIS-IR MICROSPECTROMETRY POLARIZED LIGHT MICROSCOPY
ffta T TRACE EVIDENCE ANALYSIS W O R K S T A T I O N RAMAN SPECTROSCOPY UV-VIS-IR MICROSPECTROMETRY GRIM GLASS RI MEASUREMENT FLUORESCENT IMAGING POLARIZED LIGHT MICROSCOPY DIGITAL IMAGE PROCESSING For
More informationConfocal Imaging Through Scattering Media with a Volume Holographic Filter
Confocal Imaging Through Scattering Media with a Volume Holographic Filter Michal Balberg +, George Barbastathis*, Sergio Fantini % and David J. Brady University of Illinois at Urbana-Champaign, Urbana,
More informationMicroscopic Structures
Microscopic Structures Image Analysis Metal, 3D Image (Red-Green) The microscopic methods range from dark field / bright field microscopy through polarisation- and inverse microscopy to techniques like
More informationVision Lighting Seminar
Creators of Evenlite Vision Lighting Seminar Daryl Martin Midwest Sales & Support Manager Advanced illumination 734-213 213-13121312 dmartin@advill.com www.advill.com 2005 1 Objectives Lighting Source
More informationStandard Operating Procedure of Atomic Force Microscope (Anasys afm+)
Standard Operating Procedure of Atomic Force Microscope (Anasys afm+) The Anasys Instruments afm+ system incorporates an Atomic Force Microscope which can scan the sample in the contact mode and generate
More informationScience Detectors for E-ELT Instruments. Mark Casali
Science Detectors for E-ELT Instruments Mark Casali 1 The Telescope Nasmyth telescope with a segmented primary mirror. Novel 5 mirror design to include adaptive optics in the telescope. Classical 3mirror
More informationMulti-channel imaging cytometry with a single detector
Multi-channel imaging cytometry with a single detector Sarah Locknar 1, John Barton 1, Mark Entwistle 2, Gary Carver 1 and Robert Johnson 1 1 Omega Optical, Brattleboro, VT 05301 2 Philadelphia Lightwave,
More informationAcoustic resolution. photoacoustic Doppler velocimetry. in blood-mimicking fluids. Supplementary Information
Acoustic resolution photoacoustic Doppler velocimetry in blood-mimicking fluids Joanna Brunker 1, *, Paul Beard 1 Supplementary Information 1 Department of Medical Physics and Biomedical Engineering, University
More informationEducation in Microscopy and Digital Imaging
Contact Us Carl Zeiss Education in Microscopy and Digital Imaging ZEISS Home Products Solutions Support Online Shop ZEISS International ZEISS Campus Home Interactive Tutorials Basic Microscopy Spectral
More informationGet the full picture of your sample. Applications
Follow the Experts Get the full picture of your sample The new generation of confocal Raman microscopes offers a non-destructive and non-contact method of sample analysis at the sub-micron level. More
More informationGeorgia O'Keeffe. THE Alfred Stieglitz COLLECTION OBJECT RESEARCH Palladium print Alfred Stieglitz Collection. AIC accession number: 1949.
Alfred Stieglitz (American, 1864 1946) Georgia O'Keeffe 1918 Palladium print Alfred Stieglitz Collection AIC accession number: 1949.745A Stieglitz Estate number: OK 19E Inscriptions: Unmarked recto; inscribed
More informationEQUIPMENT INFORMATION
OPTICAL (NIR) MEASURING SYSTEM UR 5500 LED EQUIPMENT INFORMATION Robert-Bosch-Straße 5 D-56566 Neuwied Tel. +49 (0) 26 31 / 96 40 00 Fax. +49 (0) 26 31 / 96 40 40 Internet: www.sensor-control.de E-Mail:
More informationFundamentals of Light Microscopy II: Fluorescence, Deconvolution, Confocal, Multiphoton, Spectral microscopy. Integrated Microscopy Course
Fundamentals of Light Microscopy II: Fluorescence, Deconvolution, Confocal, Multiphoton, Spectral microscopy Integrated Microscopy Course Review Lecture 1: Microscopy Basics Light train Kohler illumination*
More informationChemistry 524--"Hour Exam"--Keiderling Mar. 19, pm SES
Chemistry 524--"Hour Exam"--Keiderling Mar. 19, 2013 -- 2-4 pm -- 170 SES Please answer all questions in the answer book provided. Calculators, rulers, pens and pencils permitted. No open books allowed.
More informationMicroscopy: Fundamental Principles and Practical Approaches
Microscopy: Fundamental Principles and Practical Approaches Simon Atkinson Online Resource: http://micro.magnet.fsu.edu/primer/index.html Book: Murphy, D.B. Fundamentals of Light Microscopy and Electronic
More informationPractical work no. 3: Confocal Live Cell Microscopy
Practical work no. 3: Confocal Live Cell Microscopy Course Instructor: Mikko Liljeström (MIU) 1 Background Confocal microscopy: The main idea behind confocality is that it suppresses the signal outside
More informationLow Voltage Electron Microscope
LVEM5 Low Voltage Electron Microscope Nanoscale from your benchtop LVEM5 Delong America DELONG INSTRUMENTS COMPACT BUT POWERFUL The LVEM5 is designed to excel across a broad range of applications in material
More informationIn their earliest form, bandpass filters
Bandpass Filters Past and Present Bandpass filters are passive optical devices that control the flow of light. They can be used either to isolate certain wavelengths or colors, or to control the wavelengths
More informationMass transfer with elastomer stamps for microled displays.
Frontiers in Assembly Mass transfer with elastomer stamps for microled displays. Matt Meitl X-Celeprint, Inc. mmeitl@x-celeprint.com 1 The best materials for the best displays The materials identify the
More information