Confocal Microscope. Confocal Microscope C2

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1 Confocal Microscope Confocal Microscope C2

2 Confocal Microscope An essential microscopy laboratory insturument The C2 confocal microscope system comprises a new generation of Nikon confocal instruments designed to be essential laboratory tools. Built on a reputation of incredible stability and operational simplicity coupled with superior optical technologies, the C2 with its host of functions and various analytical capabilities is the perfect tool for a new microscope, or as a new accessory to a Nikon imaging system. The C2 employs Nikon s proprietary imaging software NIS-Elements, which has a high reputation for image acquisition in the camera market. NIS-Elements also allows the C2 to enjoy the same operational ease as the high-grade confocal microscope system A1. The NIS-Elements software that enhanced trust in Nikon s microscopy imaging systems is a powerful reinforcement for the C2 s usability, functionality, and broad analytical capability. 2 3

3 Image Quality Nikon's unprecedented optics and a time-proven, highly efficient optical design provide the brightest and sharpest images, at the longest working distances. High functionality Nikon's renowned imaging software NIS-Elements enables intuitive operation of all Nikon hardware devices and peripheral. With a remarkably large number of analyzing functions for its class, the C2 supports your routine laboratory research activities. Multimode capability All Nikon hardware is controlled by the same software as the top grade confocal system A1: one software package controls it all, and even at the same time! You can do confocal, widefield, TIRF, photoactivation acquisition, and processing, analysis, presentation all in one software package. Setting: Easy-to-recognize display for setting lasers, detectors, etc. Large Image Simple GUI: Simple display of fundamental image acquisition settings Scanning parameter settings High efficiency scanning heads and detectors The C2 fits all Nikon microscopes with the smallest scan head footprint on the market. The C2 employs high precision mirrors and optically ideal circular pinholes, enabling noiseless, high contrast and high quality confocal imaging, The spectral detector of the C2 enables high speed imaging using simultaneous 32-channel acquisition. Signal loss has been minimized while imaging of fluorescence spectra in real colors is possible by host of innovations for accurately correcting spectral data. High-performance optics CFI Apochromat S Series These high NA objectives are ideal for confocal imaging with correction of chromatic aberrations over a wide wavelength range, from ultra violet to infrared. Transmission is increased through the use of Nikon s exclusive Nano Crystal Coat technology. CFI Apochromat TIRF Series These objectives boast an unprecedented NA of 1.49 (using a standard coverslip and immersion oil), the highest resolution among Nikon objectives. The temperature correction ring corrects image quality affected by temperature change in the range of 23 C to 37 C. High definition diascopic DIC images CFI Apo 40xWI λs, NA1.25 (left) CFI Apo LWD 40xWI λs, NA1.15 (middle) CFI Apo 60x Oil λs, NA1.4 (right) Specimen: Genital tract of Drosophila melanogaster Photo courtesy of: Director and Professor Masatoshi Yamamoto, Drosophila Genetic Resource Center, Kyoto Institute of Technology Testis Unmixing Seminal Vesicle Accessory Gland Ejaculatory Duct 2.3mm Unmixing Ejaculatory Bulb The C2 handles simultaneous 3-channel fluorescence or simultaneous 3- channel + diascopic DIC observation. High quality DIC images and fluorescence images can be superimposed to aid in image analysis such as locating fluorescence labels. Spectral analysis GUI: Numerous functions for analysis and unmixing of acquired spectrums are provided, while spectral profiles of general dyes and fluorescent proteins are preprogrammed. The glomerulus, the main filter in mammalian 2D image of FluoCells (R) #4 from Molecular Probes CFI Apo TIRF 60x oil/1.49 (left) kidneys, double stained with GFP and FITC. Both (Invitrogen). CFI Apo TIRF 100x oil/1.49 (right) reagents have green fluorescence and emission This is a section of a mouse intestine. The actin maxima that are only a few nanometres apart. The filaments are stained with Alexa Fluor 568 phalloidin picture shows the slide after spectral unmixing using and the nuclei are stained with SYTOX Green. NIS Elements' powerful unmixing algorithms, DIC image Overlay of DIC and fluorescence images providing clearly distinguishable features. 4 5

4 Flexibility The C2 can be coupled with upright, inverted, physiological, and macro imaging microscopes and has options for combinations with various top-notch experiment systems. All can be controlled with NIS-Elements software. System diagram Laser unit Detector unit LU-LR 4-laser Power Source Rack AOM Unit 2nd, 3rd DM Spectral Detector Standard Epi-fl Detector (3-PMT) L4 L1 C-LU3EX 3-laser Unit EX LU4A 4-laser Unit Multimode imaging system TIRF/Photo activation-c2 A TIRF laser illumination module and a photo activation module are integrated to enable both imaging of single molecules with extremely high S/N ratio and imaging of the fluorescence characteristic changes of photoactivated and photo-convertible fluorescent protein. Scanner set Controller Macro confocal microscope system AZ-C2 With high-definition widefield of view images larger than 1cm with unprecedentedly high S/N ratios, the AZ-C2 allows for imaging of whole-mount specimens, such as embryos, in a single shot. It offers a combination of low and high magnification objective lenses, optical zoom and a confocal scanning zoom function, enabling continuous imaging from macro to micro. Moreover, the AZ-C2 allows deep imaging of in-vivo whole specimens. C2 Scanning Head 1st DM C2si Scanning Head 1st DM Microscope Software C2 Adapter C2 Adapter TT2 ES cells Anti-Nanog antibody (Cy3), anti-oct3/4 antibody (Alexa488) and DAPI localized in cell nuclei Photographed with the cooperation of: Hiroshi Kiyonari, Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology Ti-E/U (for Ti-U) FN1 90i/80i (for 80i) AZ100 PC Photo courtesy of: Director and Professor Masatoshi Yamamoto, Drosophila Genetic Resource Center, Kyoto Institute of Technology 6 7 X position (pixel) X position (pixel) Diascopic Detector Unit

5 L4 L1 POWER Recommended layout unit: mm (2400 or 2900) 4-laser Unit Scanning Head Standard Epi-fl Detector 650 (785) L4 L Controller Spectral Detector W W=1500mm (two 19-inch monitors) W=1000mm (24-inch monitor) Note 1) Computer table size is for reference only. Note 2) Spectral detector is unnecessary for C2 and C2si-Ready. Specifications Laser* Compatible laser Laser unit Detector Standard detector Spectral detector (option) Scanning head Scanning Scanning mode Specifications and equipment are subject to change without any notice or obligation on the part of the manufacturer. June NIKON CORPORATION WARNING Solid-state laser: 405 nm, 440 (445) nm, 488 nm, 561 (594) nm, 638 (640) nm Ar laser (457 nm/488 nm/514 nm), HeNe laser (543 nm) 3-laser module (AOM or manual modulation), 4-laser module (AOTF modulation) Fluorescent detector: 3 channel PMT, Diascopic detector: 1 channel PMT Number of channels: 32, Wavelength resolution: 2.5 nm/5 nm/10 nm Compatible with previous model C1si-Ready With 3 channel fluorescent detector: Pixel size: max.2048 x2048 pixels Scanning speed: 1 fps (512 x 512 pixels, Single Direction) max. 23 fps (512 x 32 pixels, Bi Direction, 4x Zoom) With spectral detector: Pixel size: max x 1024 pixels Scanning speed: 0.5 fps (512 x 512 pixels, Single Direction) max. 6 fps (64 x 64 pixels, Single Direction) X-Y, XY rotation, zoom, ROI, XYZ, time lapse, line, stimulation, multipoint, image stitching (large image) Pinhole Circular shape, 6 size Compatible ECLIPSE Ti-E/Ti-U inverted microscope, ECLIPSE 90i/80i upright microscope, microscopes ECLIPSE FN1 fixed stage microscope, AZ100 multi-purpose zoom microscope Software NIS-Elements C Display/image processing/analysis major features 2D/3D/4D analysis, time-lapse analysis, 3D volume rendering/orthogonal, spatial filters, image stitching, multipoint time-lapse, spectral unmixing, real-time unmixing, virtual filters, deconvolution, AVI image file output Application FRAP, FLIP, FRET, photo activation, colocalization TO ENSURE CORRECT USAGE, READ THE CORRESPONDING MANUALS CAREFULLY BEFORE USING YOUR EQUIPMENT. Monitor images are simulated. Sample images in this brochure were captured using the C1 confocal microscope system. Company names and product names appearing in this brochure are their registered trademarks or trademarks. N.B. Export of the products* in this brochure is controlled under the Japanese Foreign Exchange and Foreign Trade Law. Appropriate export procedure shall be required in case of export from Japan. *Products: Hardware and its technical information (including software) C2 NIKON CORPORATION Shin-Yurakucho Bldg., 12-1, Yurakucho 1-chome, Chiyoda-ku, Tokyo , Japan phone: fax: NIKON INSTRUMENTS INC Walt Whitman Road, Melville, N.Y , U.S.A. phone: ; NIKON (within the U.S.A. only) fax: NIKON INSTRUMENTS EUROPE B.V. Laan van Kronenburg 2, 1183 AS Amstelveen, The Netherlands phone: fax: NIKON INSTRUMENTS (SHANGHAI) CO., LTD. CHINA phone: fax: (Beijing branch) phone: fax: (Guangzhou branch) phone: fax: NIKON SINGAPORE PTE LTD SINGAPORE phone: fax: NIKON MALAYSIA SDN. BHD. MALAYSIA phone: fax: NIKON INSTRUMENTS KOREA CO., LTD. KOREA phone: fax: NIKON CANADA INC. CANADA phone: fax: NIKON FRANCE S.A.S. FRANCE phone: fax: NIKON GMBH GERMANY phone: fax: NIKON INSTRUMENTS S.p.A. ITALY phone: fax: NIKON AG SWITZERLAND phone: fax: NIKON UK LTD. UNITED KINGDOM phone: fax: NIKON GMBH AUSTRIA AUSTRIA phone: fax: NIKON BELUX BELGIUM phone: fax: Printed in Japan ( )T Code No. 2CE-SCHH-1 This brochure is printed on recycled paper made from 40% used material. En