2011/2012. Catalog of. Filter Sets for

Transcription

1 C H R O M A T E C H N O L O G Y C O R P 2011/2012 Catalog of Filter Sets for Fluorescence Microscopy

2 Table of Contents Filter Set Ordering Guide...2 Nomenclature and Spectrum Reference Chart Series - Basic Filter Sets Series - Mirrors, Generic Beamsplitters and Polarizers Series - Neutral Density Filters Series - Miscellaneous Series - Standard Filter Sets Series - QDot - Wide Excitation Series - EviTag - Wide Excitation Series - Brightfield Filter Sets Series - Live/Dead Filter Sets Series - HQ Filter Sets Series - Reef Coral Fluorescent Protein Sets Series - ET Sputtered Single Band Filter Sets Series - Dual Band Filter Sets Series - Dual Band Filter Sets With Single Band Excitation Filters Series - ET Sputtered Dual Band Filter Sets and Dual Band Sets With Single Band Exciters Series - Triple Band Filter Sets Series - Triple Band Filter Sets With Single Band Excitation Filters Series - ET Sputtered Triple Band Filter Sets and Triple Band Sets with Single Band Exciters Series - Dual Excitation and Dual Emission Dye Filter Sets Series - ET Sputtered Dual Excitation and Dual Emission Dye Filter Sets Series - Multi-Band Filter Sets with Single Emitters and Multiple Exciters Series - Multi-Band Filter Sets with Single Emitters and Single Exciters Series - Quad Filter Set with Single Band Exciters Series - ET Sputtered Multi-Band Sets Series - Holders (Cubes & Rings) Series - Diagnostic Tools G-Series - Modified goggles for macro viewing MF100 - Filter Sets for Multi-Color FISH (Spec d by Perceptive Scientific for Vysis fluorochromes) SP100 - Filter Sets for Multi-Color FISH Laser Applications...81 Absorption Glass...81 Excitation Intensity Note...82 Discontinued and Special Order Sets Fluorochrome List J u n e

3 C h r o m a Te c h n o l o g y Co r p filter set ordering guide This price list provides information about the filter sets Chroma Technology has created for the multitude of fluorochromes that are typically used in epi-fluorescence microscopy. We are aware that there are applications which require filter combinations that are not listed. Often we can create such sets out of the large collection of special-order filters and dichroic beamsplitters that we maintain in inventory. Occasionally we have to manufacture newly designed filters and/or mirrors. We understand the limitations of the research budget and in either situation we try to keep prices within reasonable limits. Please contact us for help in creating filter combinations that you do not find in the catalog. Additionally, Chroma manufactures filters for a variety of instruments including gene chip readers, high-throughput systems, plate readers, flow cytometers, confocal and multi-photon microscopes, Raman spectroscopy and single-molecule microscopy. Often the manufacturers of these instruments provide specifications for the filters that they recommend. Sometimes you may wish to order filters that are different from their recommendations and sometimes the manufacturers of bioassays have their own recommendations. Please contact us for custom sets and recommended filter sets for standard bioassays. HOW TO ORDER FILTERS Answers to the following questions are extremely helpful when discussing the appropriate filters or filter sets for your use: 1. Which fluorochrome(s) is (are) being used? 2. What is the wavelength range of excitation and emission for those fluorochromes with which we are not familiar? 3. What is the make and model of the microscope or instrument being used? 4. What is the size of the excitation and/or emission filters if a filter wheel is being used in either the excitation or emission path? 5. Does the filter need to be image quality or not? This is particularly important for absorption-type emission filters. 6. What is the maximum thickness possible in the filter mount to be used? 7. What type of detector is being used (e.g. PMT, CCD, film)? 8. What are the specific reflection and transmission requirements for the dichroic beamsplitter? Dichroic beamsplitters are designed with various spectral profiles. No dichroic beamsplitter reflects all wavelengths short of the cut-on wavelengths, nor can it transmit all wavelengths beyond that point. Being as specific as possible will increase your chances of getting the beamsplitter that you require and will diminish the possibility of paying too much for a beamsplitter that is more than you require. It is best if we know the excitation and emission filters being used. This information is particularly important when ordering a dichroic beamsplitter in the absence of any other filters. FILTER PRICING The pricing in this catalog is for standard size filters for typical fluorescence microscopes including all excitation and emission filters 25 mm or smaller. Please contact us for pricing on larger size filters and appropriate mounting hardware. 2 C h r o m a T e c h n o l o g y C o r p

4 P r i c e Li s t o f Fi l t e r s NOMENCLATURE Excitation filter D480/30x The center wavelength is at 480 nm; full bandwidth is 30 nm [ = ±15]. In some cases when the bandwidth is not specified, the letter x is used to define the filter as an excitation filter. This is generally used for narrow band UV excitation filters, e.g. D340x. Dichroic beamsplitter 505DCLP The cut-on wavelength (50% transmission point) is approximately 505 nm for this longpass dichroic design. Dichroic mirrors may be named for the 50% point, but the actual reflection and transmission wavelengths are what really define the mirror. Therefore, a mirror that is called a 505DCLP may vary somewhat in the 50% transmission point. Feel confident that we will design the mirrors, regardless of name, to reflect and transmit the necessary bands. It would be much more accurate to name the mirrors for the reflection/ transmission regions, but this is very cumbersome and we have not figured out the best way to accomplish this just yet. Emission filter D535/40m The center wavelength is at 535 nm; full bandwidth is 40 nm [ = ±20]. LP longpass filter, which transmits wavelengths longer than the cut-on and blocks shorter wavelengths SP shortpass filter, which transmits wavelengths shorter than the cut-on and blocks longer wavelengths BP bandpass DCLP dichroic longpass DCXR dichroic longpass, extended reflection DCXRU dichroic longpass, extended reflection including the UV PC polychroic beamsplitter, reflects and transmits more than two bands of light GG Green Glass longpass absorption glass from Schott Glassworks with cut-on wavelengths in the violet and bluegreen regions OG Orange Glass longpass absorption glass from Schott Glassworks with cut-on wavelengths in the green, yellow and orange regions RG Red Glass longpass absorption glass from Schott Glassworks with cut-on wavelengths in the red and far red regions EX excitation filter BS beamsplitter EM emission filter ET indicates sputter coated filter or set SPR Sub-pixel registered these sets have been sub-pixel registered to provide the best possible image results and are sold only as complete sets. SPECTRUM REFERENCE CHART ultraviolet violet blue green yellow orange red near infrared (invisible) (visible) (visible) (visible) (visible) (visible) (visible) (invisible) ET-GFP (FITC/Cy2) exciter dichroic emitter ET470/40x T495LP ET525/50m The list on the left provides the item number of the filter set along with its component parts and its spectral characteristics. The graph on the right shows the spectral trace of this filter set with each component shown in a different color. These spectral traces can be found on our web site, J u n e

5 C h r o m a Te c h n o l o g y Co r p Series - Basic Filter Sets The most fundamental of Chroma s filter sets. These sets were designed primarily for viewing the fluorescence of single fluorochrome. All of these sets contain longpass emission filters. Longpass emission filters cannot exclude the fluorescence of a second fluorochrome which may also be excited. For instance, blue light, which is the primary excitation color for FITC, will also excite, albeit slightly less efficiently, than TRITC. A longpass emission filter will be unable to exclude the fluorescence of TRITC. Most of the barrier filters of our Basic sets are made from absorptive glass v3 UV Wide UV excitation with Longpass Emission AT350/50x Exciter (EX) $ DCLP Dichroic (BS) $ E420LPv2 Emitter (EM) $ Total...$ v2 Blue D470/40x Exciter (EX) $ DCXT Dichroic (BS) $ E515LPv2 Emitter (EM) $ Total...$ v2 Green Recommended for use with mercury lamps only. D546/10x Exciter (EX) $ DCXT Dichroic (BS) $ E590LPv2 Emitter (EM) $ Total...$ v2 Blue/Violet D425/40x Exciter (EX) $ DCXT Dichroic (BS) $ E475LPv2 Emitter (EM) $ Total...$ v2 Narrow UV Recommended for use with mercury lamps only. D365/10x Exciter (EX) $ DCLP Dichroic (BS) $ E400LPv2 Emitter (EM) $ Total...$ v2 Violet D405/40x Exciter (EX) $ DCXT Dichroic (BS) $ E455LPv2 Emitter (EM) $ Total...$ C h r o m a T e c h n o l o g y C o r p

6 P r i c e Li s t o f Fi l t e r s Series - Basic Filter Sets The most fundamental of Chroma s filter sets. These sets were designed primarily for viewing the fluorescence of single fluorochrome. All of these sets contain longpass emission filters. Longpass emission filters cannot exclude the fluorescence of a second fluorochrome which may also be excited. For instance, blue light, which is the primary excitation color for FITC, will also excite, albeit slightly less efficiently, than TRITC. A longpass emission filter will be unable to exclude the fluorescence of TRITC. Most of the barrier filters of our Basic sets are made from absorptive glass v3 Gold AT350/50x Exciter (EX) $ DCLP Dichroic (BS) $ E515LPv2 Emitter (EM) $ Total...$ v2 Wide Green D535/50x Exciter (EX) $ DCXT Dichroic (BS) $ E590LPv2 Emitter (EM) $ Total...$ v2 Narrow Blue/Violet Recommended for use with mercury lamps only v2 Photo-Resist Recommended for use with mercury lamps only. D436/10x Exciter (EX) $ DCXT Dichroic (BS) $ E475LPv2 Emitter (EM) $ Total...$ D365/10x Exciter (EX) $ DCLP Dichroic (BS) $ E570LPv2 Emitter (EM) $ Total...$ v2 Yellow D560/40x Exciter (EX) $ DCLP Dichroic (BS) $ E610LPv2 Emitter (EM) $ Total...$ v3 UV/Violet D390/70x Exciter (EX) $ DCXT Dichroic (BS) $ E475LPv2 Emitter (EM) $ Total...$ J u n e

7 C h r o m a Te c h n o l o g y Co r p Series - Basic Filter Sets The most fundamental of Chroma s filter sets. These sets were designed primarily for viewing the fluorescence of single fluorochrome. All of these sets contain longpass emission filters. Longpass emission filters cannot exclude the fluorescence of a second fluorochrome which may also be excited. For instance, blue light, which is the primary excitation color for FITC, will also excite, albeit slightly less efficiently, than TRITC. A longpass emission filter will be unable to exclude the fluorescence of TRITC. Most of the barrier filters of our Basic sets are made from absorptive glass v2 Wide Blue D455/70x Exciter (EX) $ dcxr Dichroic (BS) $ E515LPv2 Emitter (EM) $ Total...$ v2 Narrow Blue D480/20x Exciter (EX) $ DCXT Dichroic (BS) $ E515LPv2 Emitter (EM) $ Total...$ C h r o m a T e c h n o l o g y C o r p

8 P r i c e Li s t o f Fi l t e r s Series - Mirrors, Generic Beamsplitters and Polarizers /50 Beamsplitter Allows 50% Reflection / 50% Transmission 50/50 Beamsplitter Dichroic (BS) $ Total...$ /20 Beamsplitter Allows 80% Reflection / 20% Transmission 80/20 Beamsplitter Dichroic (BS) $ Total...$ a Hot Mirror - up to 25mm Hot Mirror $50.00 Total...$ b Hot Mirror 26mm - 50mm Hot Mirror $75.00 Total...$ a Polarizer - up to 25mm Total...$ b Polarizer - 26mm to 50mm Total...$ J u n e

9 C h r o m a Te c h n o l o g y Co r p Series - Mirrors, Generic Beamsplitters and Polarizers Fully Reflective Mirror Fully Reflective Mirror $ Total...$ Cold Mirror Not designed for imaging. Do not use in emission path or with lasers. Cold Mirror $75.00 Total...$ /30 Beamsplitter Allows 70% Reflection / 30% Transmission 70/30 Beamsplitter Dichroic (BS) $ Total...$ /80 Beamsplitter Allows 20% Reflection / 80% Transmission 20/80 Beamsplitter Dichroic (BS) $ Total...$ /70 Beamsplitter Allows 30% Reflection / 70% Transmission 30/70 Beamsplitter Dichroic (BS) $ Total...$ Enhanced Silver Reflective Mirror Enhanced Silver Reflective Mirror $ Total...$ C h r o m a T e c h n o l o g y C o r p

10 P r i c e Li s t o f Fi l t e r s Series - Mirrors, Generic Beamsplitters and Polarizers /10 Beamsplitter Allows 90% Reflection / 10% Transmission 90/10 Beamsplitter Dichroic (BS) $ Total...$ /90 Beamsplitter Allows 10% Reflection / 90% Transmission 10/90 Beamsplitter Dichroic (BS) $ Total...$ J u n e

11 C h r o m a Te c h n o l o g y Co r p Series - Neutral Density Filters Neutral Density Filter Series Please indicate size required b Neutral Density Filter Series (26mm - 36mm) Please indicate size required. UVND % transmission $60.00 UVND % transmission $60.00 UVND % transmission $60.00 UVND % transmission $60.00 UVND % transmission $60.00 UVND % transmission $60.00 UVND % transmission $60.00 UVND % transmission $60.00 UVND % transmission $60.00 UVND % transmission $60.00 UVND % transmission $60.00 Total... UVND 0.1 B 80.0% transmission $ UVND 0.2 B 63.0% transmission $ UVND 0.3 B 50.0% transmission $ UVND 0.4 B 40.0% transmission $ UVND 0.5 B 32.0% transmission $ UVND 0.6 B 25.0% transmission $ UVND 1.0 B 10.0% transmission $ UVND 1.3 B 5.0% transmission $ UVND 1.5 B 3.0% transmission $ UVND 2.0 B 1.0% transmission $ UVND 3.0 B 0.1% transmission $ Total C h r o m a T e c h n o l o g y C o r p

12 P r i c e Li s t o f Fi l t e r s Series - Neutral Density Filters 22000c Neutral Density Filter Series (37mm - 50mm) Please indicate size required. UVND 0.1 C 80.0% transmission $ UVND 0.2 C 63.0% transmission $ UVND 0.3 C 50.0% transmission $ UVND 0.4 C 40.0% transmission $ UVND 0.5 C 32.0% transmission $ UVND 0.6 C 25.0% transmission $ UVND 1.0 C 10.0% transmission $ UVND 1.3 C 5.0% transmission $ UVND 1.5 C 3.0% transmission $ UVND 2.0 C 1.0% transmission $ UVND 3.0 C 0.1% transmission $ Total... J u n e

13 C h r o m a Te c h n o l o g y Co r p Series - Miscellaneous RGB Filter Set Emission filters for acquiring B&W images in pseudo-color. D470/38m Emitter (EM) $ D535/38m Emitter (EM) $ D630/60m Emitter (EM) $ Total...$ C h r o m a T e c h n o l o g y C o r p

14 P r i c e Li s t o f Fi l t e r s Series - Standard Filter Sets By replacing the longpass emission filter of the 11/Basic Series with bandpass emission filters Chroma s Standard series prevents bleed-through of undesired long wavelength fluorescence: for example, preventing red fluorescence from appearing in the green image). These filters also tend to decrease the problems associated with autofluorescence v2 DAPI/Hoechst/AMCA See also the ET sputtered set FITC/RSGFP/Fluo 3/DiO Acradine Orange(+DNA) AT350/50x Exciter (EX) $ DCLP Dichroic (BS) $ D460/50m Emitter (EM) $ Total...$ D480/30x Exciter (EX) $ Q505LP Dichroic (BS) $ D535/40m Emitter (EM) $ Total...$ TRITC (Rhodamine)/DiI/Cy3 D540/25x Exciter (EX) $ Q565LP Dichroic (BS) $ D605/55m Emitter (EM) $ Total...$ a TRITC (Rhodamine)/DiI/Cy3 For redder signal Will result in a redder image by eye as compared to the set. Will also result in a loss of total signal with Rhodamine-like dyes as compared to the set R and B Phycoerythrin (PE) Hq Light Source Recommended for use with mercury lamps only. D540/25x Exciter (EX) $ Q565LP Dichroic (BS) $ D620/60m Emitter (EM) $ Total...$ D546/10x Exciter (EX) $ DCLP Dichroic (BS) $ D580/30m Emitter (EM) $ Total...$ Texas Red / Cy3.5 D560/40x Exciter (EX) $ Q595LP Dichroic (BS) $ D630/60m Emitter (EM) $ Total...$ J u n e

15 C h r o m a Te c h n o l o g y Co r p Series - Standard Filter Sets By replacing the longpass emission filter of the 11/Basic Series with bandpass emission filters Chroma s Standard series prevents bleed-through of undesired long wavelength fluorescence: for example, preventing red fluorescence from appearing in the green image). These filters also tend to decrease the problems associated with autofluorescence Allophycocyanin (APC) The emission at these wavelengths will be better detected by a camera than by the unaided eye. Only a small percentage of humans can detect these wavelengths. This set has been discontinued. Please see replacement set Quinacrine Mustard This set has been discontinued. Please see replacement set Lucifer Yellow This set replaces discontinued set: Acridine Orange (+RNA) This set is designed to image Acridine Orange bound to RNA v2 Narrow Band Excitation Set for DAPI/Hoechst/AMCA Recommended for use with mercury lamps only Hydroxycoumarin This set has been discontinued. Please see replacement set D595/40x Exciter (EX) $ DCLP Dichroic (BS) $ D660/40m Emitter (EM) $ Total... D425/40x Exciter (EX) $ DCLP Dichroic (BS) $ D540/50m Emitter (EM) $ Total... D425/40x Exciter (EX) $ DCLP Dichroic (BS) $ D540/50m Emitter (EM) $ Total...$ D480/60x Exciter (EX) $ dcxr Dichroic (BS) $ D630/60m Emitter (EM) $ Total...$ D365/10x Exciter (EX) $ DCLP Dichroic (BS) $ D460/50m Emitter (EM) $ Total...$ D405/20x Exciter (EX) $ DCLP Dichroic (BS) $ D460/50m Emitter (EM) $ Total C h r o m a T e c h n o l o g y C o r p

16 P r i c e Li s t o f Fi l t e r s Series - Standard Filter Sets By replacing the longpass emission filter of the 11/Basic Series with bandpass emission filters Chroma s Standard series prevents bleed-through of undesired long wavelength fluorescence: for example, preventing red fluorescence from appearing in the green image). These filters also tend to decrease the problems associated with autofluorescence Chlorophyll Bandpass Emission This set has been discontinued. Please see replacement set a Chlorophyll Longpass Emission This set has been discontinued. Please see replacement set Di-8-ANEPPS (non ratio) Designed for non-ratio imaging of Di-4-ANEPPS and Di-8-ANEPPS. This set replaces discontinued set: Blue GFP Bandpass Emission This set has been discontinued. Please see replacement set DiA This set has been discontinued. Please see replacement set D480/30x Exciter (EX) $ DCXT Dichroic (BS) $ D660/50m Emitter (EM) $ Total... D480/30x Exciter (EX) $ DCXT Dichroic (BS) $ E600LP Emitter (EM) $ Total... D480/30x Exciter (EX) $ Q505LP Dichroic (BS) $ D605/54m Emitter (EM) $ Total...$ D390/22x Exciter (EX) $ DCLP Dichroic (BS) $ D460/50m Emitter (EM) $ Total... D480/30x Exciter (EX) $ DCLP Dichroic (BS) $ D605/54m Emitter (EM) $ Total... J u n e

17 C h r o m a Te c h n o l o g y Co r p Series - Standard Filter Sets By replacing the longpass emission filter of the 11/Basic Series with bandpass emission filters Chroma s Standard series prevents bleed-through of undesired long wavelength fluorescence: for example, preventing red fluorescence from appearing in the green image). These filters also tend to decrease the problems associated with autofluorescence Narrow-band GFP for eliminating auto-fluorescence This set will, in some cases, increase the signal-to-noise ratio minimizing autofluorescence of GFP imaged in live cells with high levels of autofluorescence. It will decrease the GFP signal substantially and should not be used with dim samples. This set has been discontinued. Please see replacement set CTC by special request v2 DEAC by special request. Also refer to replacement set D480/20x Exciter (EX) $ DCLP Dichroic (BS) $ D520/20m Emitter (EM) $ Total... D450/50x Exciter (EX) $ DCLP Dichroic (BS) $ D630/60m Emitter (EM) $ Total...$ D436/20x Exciter (EX) $ DCLP Dichroic (BS) $ D480/30m Emitter (EM) $ Total...$ Pacific Blue D410/40x Exciter (EX) $ DCLP Dichroic (BS) $ D470/40m Emitter (EM) $ Total...$ Blue GFP II D380/30x Exciter (EX) $ DCLP Dichroic (BS) $ D460/50m Emitter (EM) $ Total...$ C h r o m a T e c h n o l o g y C o r p

18 P r i c e Li s t o f Fi l t e r s Series - Standard Filter Sets By replacing the longpass emission filter of the 11/Basic Series with bandpass emission filters Chroma s Standard series prevents bleed-through of undesired long wavelength fluorescence: for example, preventing red fluorescence from appearing in the green image). These filters also tend to decrease the problems associated with autofluorescence Sapphire/UV GFP D395/40x Exciter (EX) $ DCLP Dichroic (BS) $ D510/40m Emitter (EM) $ Total...$ v2 CFP/DEAC This set will typically exclude the signal from YFP but not from GFP. See also the ET sputtered set Red This set has been discontinued. Please see replacement set v2 Coumarin This set has been discontinued. Please see replacement set Cyan/Topaz Energy Transfer FRET is extremely difficult in wide-field microscopy. Please see our FRET application note for more information. This set has been discontinued. Please see replacement set v2 Fast Red by special request. D436/20x Exciter (EX) $ DCLP Dichroic (BS) $ D480/40m Emitter (EM) $ Total...$ D577/20x Exciter (EX) $ Q595LP Dichroic (BS) $ D630/30m Emitter (EM) $ Total... D405/20x Exciter (EX) $ DCLP Dichroic (BS) $ D460/50m Emitter (EM) $ Total... D436/20x Exciter (EX) $ DCLP Dichroic (BS) $ D535/30m Emitter (EM) $ Total... AT350/50x Exciter (EX) $ DCLP Dichroic (BS) $ E550LP Emitter (EM) $ Total...$ J u n e

19 C h r o m a Te c h n o l o g y Co r p Series - QDot - Wide Excitation This series contains the original filter sets designed for Quantum Dot Corp. These sets are specifically developed with wide excitation to capture all of the energy from UV to blue. Note: this wide excitation may not be necessary for all applications, and could result in increased autofluorescence GFP Bandpass Emission by special request. D425/60x Exciter (EX) $ dcxr Dichroic (BS) $ D505/40m Emitter (EM) $ Total...$ Qdot 605 with 40nm emission filter E460SPUVv2 Exciter (EX) $ dcxru Dichroic (BS) $ D605/40m Emitter (EM) $ Total...$ Qdot 585 with 40nm emission filter E460SPUVv2 Exciter (EX) $ dcxru Dichroic (BS) $ D585/40m Emitter (EM) $ Total...$ Qdot 565 with 40nm emission filter E460SPUVv2 Exciter (EX) $ dcxru Dichroic (BS) $ D565/40m Emitter (EM) $ Total...$ Qdot 525 with 40nm emission filter E460SPUVv2 Exciter (EX) $ dcxru Dichroic (BS) $ D525/40m Emitter (EM) $ Total...$ Qdot 655 with 40nm emission filter E460SPUVv2 Exciter (EX) $ dcxru Dichroic (BS) $ D655/40m Emitter (EM) $ Total...$ C h r o m a T e c h n o l o g y C o r p

20 P r i c e Li s t o f Fi l t e r s Series - QDot - Wide Excitation This series contains the original filter sets designed for Quantum Dot Corp. These sets are specifically developed with wide excitation to capture all of the energy from UV to blue. Note: this wide excitation may not be necessary for all applications, and could result in increased autofluorescence Qdot set with longpass emission filter E460SPUVv2 Exciter (EX) $ dcxru Dichroic (BS) $ E500LP Emitter (EM) $ Total...$ Qdot 705 filter set with 40nm wide emission E460SPUVv2 Exciter (EX) $ dcxru Dichroic (BS) $ D705/40m Emitter (EM) $ Total...$ Qdot 755 filter set with 40nm wide emission E460SPUVv2 Exciter (EX) $ dcxru Dichroic (BS) $ D755/40m Emitter (EM) $ Total...$ Qdot 850 filter set with 40 nm wide emission E460SPUVv2 Exciter (EX) $ dcxru Dichroic (BS) $ D850/40m Emitter (EM) $ Total...$ Qdot 800 E460SPUVv2 Exciter (EX) $ dcxru Dichroic (BS) $ D800/50m Emitter (EM) $ Total...$ J u n e

21 C h r o m a Te c h n o l o g y Co r p Series - EviTag - Wide Excitation This series contains the original filter sets designed for Evident Technologies. These sets are specifically developed with wide excitation to capture all of the energy from UV to blue. Note: this wide excitation may not be necessary for all applications, and could result in increased autofluorescence Catskill Green E460SPUVv2 Exciter (EX) $ dcxru Dichroic (BS) $ D540/40m Emitter (EM) $ Total...$ Maple Red Orange E460SPUVv2 Exciter (EX) $ dcxru Dichroic (BS) $ D620/40m Emitter (EM) $ Total...$ C h r o m a T e c h n o l o g y C o r p

22 P r i c e Li s t o f Fi l t e r s Series - Brightfield Filter Sets IGS Standard This filter set is designed to image gold and silver particles utilizing polarizing filters such as the GG420 glass to block UV from harming eyes and KG5 to block the IR v2 Brightfield For using epi-fluorescence illumination to visualize sample with white light. The E420LPv2 blocks UV light from harming the eye. GG420+Polarizer+KG5 Exciter (EX) $ /50 Beamsplitter Dichroic (BS) $ Polarizer Emitter (EM) $ Total...$ /50 Beamsplitter Dichroic (BS) $ E420LPv2 Emitter (EM) $ KG1 Emitter (EM) $50.00 Total...$ J u n e

23 C h r o m a Te c h n o l o g y Co r p Series - Live/Dead Filter Sets Live/Dead sets for determining cytotoxicity using Molecular Probes Reagents v3 Green/Orange For use with two-color cell viability assays with dyes similar to FITC and Rhodamine v2 Live/Dead (Green/Red) For use with two-color cell viability assays with dyes similar to FITC and Texas Red. D470/30x Exciter (EX) $ Q495LP Dichroic (BS) $ v2m Emitter (EM) $ Total...$ D480/30x Exciter (EX) $ Q505LP Dichroic (BS) $ m Emitter (EM) $ Total...$ C h r o m a T e c h n o l o g y C o r p

24 P r i c e Li s t o f Fi l t e r s Series - HQ Filter Sets This filter series was designed to increase brightness without a corresponding increase in noise. By increasing the number of interference cavities in both the excitation and emission filters, we have created filters with steeper passbands. This allowed wider excitation and emission filters which were spectrally closer together than the Standard series. Wider and steeper filters provide more excitation and capture more fluorescence, resulting in greater signal to noise. This unique design also allows for the excitation/dichroic/emissions to be placed closer together for those dyes with a very short Stoke s shift FITC/ RSGFP/ Bodipy/ Fluo 3/ DiO See also the ET sputtered set TRITC (Rhodamine)/DiI/Cy3 This set has been discontinued. Please see replacement set a TRITC (Rhodamine)/DiI with red-shifted emission filter Will result in a redder image by eye as compared to the set. Will also result in a loss of total signal with Rhodamine-like dyes as compared to the set. See also the ET sputtered set This set has been discontinued. Please see replacement set b TRITC (Rhodamine)/DiI/Cy3 /AF546 with narrow-band excitation filter 41002c TRITC (Rhodamine) with narrow-band excitation filter and red-shifted emission filter HQ480/40x Exciter (EX) $ Q505LP Dichroic (BS) $ HQ535/50m Emitter (EM) $ Total...$ HQ535/50x Exciter (EX) $ Q565LP Dichroic (BS) $ HQ610/75m Emitter (EM) $ Total... HQ535/50x Exciter (EX) $ Q565LP Dichroic (BS) $ HQ620/60m Emitter (EM) $ Total... HQ545/30x Exciter (EX) $ Q570LP Dichroic (BS) $ HQ610/75m Emitter (EM) $ Total...$ HQ545/30x Exciter (EX) $ Q570LP Dichroic (BS) $ HQ620/60m Emitter (EM) $ Total...$ J u n e

25 C h r o m a Te c h n o l o g y Co r p Series - HQ Filter Sets This filter series was designed to increase brightness without a corresponding increase in noise. By increasing the number of interference cavities in both the excitation and emission filters, we have created filters with steeper passbands. This allowed wider excitation and emission filters which were spectrally closer together than the Standard series. Wider and steeper filters provide more excitation and capture more fluorescence, resulting in greater signal to noise. This unique design also allows for the excitation/dichroic/emissions to be placed closer together for those dyes with a very short Stoke s shift R and B Phycoerythrin Hg light source Recommended for use with mercury lamps only. This set has been discontinued. Please see replacement set Texas Red See also the ET sputtered set HQ546/11x Exciter (EX) $ Q560LP Dichroic (BS) $ HQ585/40m Emitter (EM) $ Total... HQ560/55x Exciter (EX) $ Q595LP Dichroic (BS) $ HQ645/75m Emitter (EM) $ Total...$ Propidium Iodide HQ535/50x Exciter (EX) $ Q565LP Dichroic (BS) $ HQ645/75m Emitter (EM) $ Total...$ Ethidium Bromide This set has been discontinued. Please see replacement set Cy3 /TRITC (Rhodamine)/DiI The wide excitation filter can sometimes excite fluorochromes like FITC in dual-stain applications. See also the ET sputtered set This set replaces discontinued sets: 41002, a Cy3 (With narrow band excitation filter) This is the recommended Cy3 filter set. HQ535/50x Exciter (EX) $ Q565LP Dichroic (BS) $ HQ610/75m Emitter (EM) $ Total... HQ535/50x Exciter (EX) $ Q565LP Dichroic (BS) $ HQ610/75m Emitter (EM) $ Total...$ HQ545/30x Exciter (EX) $ Q570LP Dichroic (BS) $ HQ610/75m Emitter (EM) $ Total...$ C h r o m a T e c h n o l o g y C o r p

26 P r i c e Li s t o f Fi l t e r s Series - HQ Filter Sets This filter series was designed to increase brightness without a corresponding increase in noise. By increasing the number of interference cavities in both the excitation and emission filters, we have created filters with steeper passbands. This allowed wider excitation and emission filters which were spectrally closer together than the Standard series. Wider and steeper filters provide more excitation and capture more fluorescence, resulting in greater signal to noise. This unique design also allows for the excitation/dichroic/emissions to be placed closer together for those dyes with a very short Stoke s shift Cy5 The emission at these wavelengths will be better detected by camera than by the unaided eye. Only a small percentage of humans can detect these wavelengths Cy7 Light passed through this emission filter cannot be seen by eye and must be detected with an IR-sensitive detector. This set has been discontinued. Please see replacement set Resorufin Recommended for use with mercury lamps only. This set has been discontinued. Please see replacement set HQ620/60x Exciter (EX) $ Q660LP Dichroic (BS) $ HQ700/75m Emitter (EM) $ Total...$ HQ710/75x Exciter (EX) $ Q750LP Dichroic (BS) $ HQ810/90m Emitter (EM) $ Total... HQ577/10x Exciter (EX) $ Q585LP Dichroic (BS) $ HQ620/60m Emitter (EM) $ Total Eosin/ZsYellow HQ500/40x Exciter (EX) $ Q530LP Dichroic (BS) $ HQ560/50m Emitter (EM) $ Total...$ FITC/RSGFP LP Emission Longpass filter sets may decrease your signal-to-noise ratio on samples with high auto-fluorescence Allophycocyanin (APC) The emission at these wavelengths will be better detected by camera than by the unaided eye. Only a small percentage of humans can detect these wavelengths. This set replaces discontinued sets: 31006, HQ480/40x Exciter (EX) $ DCXT Dichroic (BS) $ HQ510LP Emitter (EM) $ Total...$ HQ590/55x Exciter (EX) $ Q625LP Dichroic (BS) $ HQ665/65m Emitter (EM) $ Total...$ J u n e

27 C h r o m a Te c h n o l o g y Co r p Series - HQ Filter Sets This filter series was designed to increase brightness without a corresponding increase in noise. By increasing the number of interference cavities in both the excitation and emission filters, we have created filters with steeper passbands. This allowed wider excitation and emission filters which were spectrally closer together than the Standard series. Wider and steeper filters provide more excitation and capture more fluorescence, resulting in greater signal to noise. This unique design also allows for the excitation/dichroic/emissions to be placed closer together for those dyes with a very short Stoke s shift wtgfp Bandpass Emission HQ450/50x Exciter (EX) $ Q480LP Dichroic (BS) $ HQ510/50m Emitter (EM) $ Total...$ wtgfp Longpass Emission Longpass filter sets may decrease your signal-to-noise ratio on samples with high auto-fluorescence. by special request Endow GFP Bandpass Emission This is the recommended filter set for the newer S65T GFP mutants. It is also recommended for wtgfp for which it was originally designed Endow GFP Longpass Emission Longpass filter sets may decrease your signal-to-noise ratio on samples with high auto-fluorescence Visual Cy5 (GLF) Designed by G.L. Ferri for visual analysis of Cy5. The signal obtained with this set will be much weaker than the standard Cy5 filter set and may not work in many applications. by special request. Also refer to replacement set HQ450/50x Exciter (EX) $ Q480LP Dichroic (BS) $ HQ485LP Emitter (EM) $ Total...$ HQ470/40x Exciter (EX) $ Q495LP Dichroic (BS) $ HQ525/50m Emitter (EM) $ Total...$ HQ470/40x Exciter (EX) $ Q495LP Dichroic (BS) $ HQ500LP Emitter (EM) $ Total...$ HQ615/45x Exciter (EX) $ Q646LP Dichroic (BS) $ HQ679/60m Emitter (EM) $ Total...$ C h r o m a T e c h n o l o g y C o r p

28 P r i c e Li s t o f Fi l t e r s Series - HQ Filter Sets This filter series was designed to increase brightness without a corresponding increase in noise. By increasing the number of interference cavities in both the excitation and emission filters, we have created filters with steeper passbands. This allowed wider excitation and emission filters which were spectrally closer together than the Standard series. Wider and steeper filters provide more excitation and capture more fluorescence, resulting in greater signal to noise. This unique design also allows for the excitation/dichroic/emissions to be placed closer together for those dyes with a very short Stoke s shift Narrow-band GFP for eliminating auto-fluorescence This set will, in some cases, increase the signal-to-noise ratio of GFP imaged in live cells with high levels of autofluorescence. It will decrease the GFP signal substantially and should not be used with dim samples. This set replaces discontinued set: HQ480/20x Exciter (EX) $ Q495LP Dichroic (BS) $ HQ510/20m Emitter (EM) $ Total...$ Cy3.5 HQ565/30x Exciter (EX) $ Q585LP Dichroic (BS) $ HQ620/60m Emitter (EM) $ Total...$ Cy5.5 (Red Shifted) Light passed through this emission filter cannot be seen by eye and must be detected with an IR-sensitive detector. by special request. Also refer to replacement set Cy5.5 Light passed through this emission filter cannot be seen by eye and must be detected with an IR-sensitive detector. This set replaces discontinued set: Cy5 Longpass Emission The emission at these wavelengths will be better detected by camera than by the unaided eye. Only a small percentage of humans can detect these wavelengths. HQ665/45x Exciter (EX) $ Q695LP Dichroic (BS) $ HQ725/50m Emitter (EM) $ Total...$ HQ650/45x Exciter (EX) $ Q680LP Dichroic (BS) $ HQ710/50m Emitter (EM) $ Total...$ HQ620/60x Exciter (EX) $ Q660LP Dichroic (BS) $ HQ665LP Emitter (EM) $ Total...$ J u n e

29 C h r o m a Te c h n o l o g y Co r p Series - HQ Filter Sets This filter series was designed to increase brightness without a corresponding increase in noise. By increasing the number of interference cavities in both the excitation and emission filters, we have created filters with steeper passbands. This allowed wider excitation and emission filters which were spectrally closer together than the Standard series. Wider and steeper filters provide more excitation and capture more fluorescence, resulting in greater signal to noise. This unique design also allows for the excitation/dichroic/emissions to be placed closer together for those dyes with a very short Stoke s shift Piston GFP Bandpass This set was designed by Dave Piston to minimize auto-fluorescence of flavins in GFP imaging. HQ470/40x Exciter (EX) $ Q495LP Dichroic (BS) $ HQ515/30m Emitter (EM) $ Total...$ Oregon Green HQ495/30x Exciter (EX) $ Q516LP Dichroic (BS) $ HQ545/50m Emitter (EM) $ Total...$ Calcium Crimson by special request. Also refer to replacement set Yellow GFP BP (10C/Topaz) This set is designed to image YFP. It will not, in most cases, detect CFP when co-expressed in cells. By eye, fluorescence in the 535 nm passband will be seen as green Yellow GFP LP (10/C Topaz) Longpass filter sets may decrease your signal-to-noise ratio on samples with high auto-fluorescence. by special request. Also refer to replacement set Indo-Cyanine Green This set requires a detector with good QE in the nm range. This set has been discontinued. Please see replacement set HQ580/20x Exciter (EX) $ Q595LP Dichroic (BS) $ HQ630/60m Emitter (EM) $ Total...$ HQ500/20x Exciter (EX) $ Q515LP Dichroic (BS) $ HQ535/30m Emitter (EM) $ Total...$ HQ500/20x Exciter (EX) $ Q515LP Dichroic (BS) $ HQ520LP Emitter (EM) $ Total...$ HQ775/50x Exciter (EX) $ Q810LP Dichroic (BS) $ HQ845/55m Emitter (EM) $ Total C h r o m a T e c h n o l o g y C o r p

30 P r i c e Li s t o f Fi l t e r s Series - HQ Filter Sets This filter series was designed to increase brightness without a corresponding increase in noise. By increasing the number of interference cavities in both the excitation and emission filters, we have created filters with steeper passbands. This allowed wider excitation and emission filters which were spectrally closer together than the Standard series. Wider and steeper filters provide more excitation and capture more fluorescence, resulting in greater signal to noise. This unique design also allows for the excitation/dichroic/emissions to be placed closer together for those dyes with a very short Stoke s shift Beta-Lactamase, Blue/Aqua This longpass set was originally spec d by the Tsien lab for visualization of coumarin and fluorescein in Beta- Lactamase assays. It is not designed for ratiometric imaging Rhodamine LP Longpass filter sets may decrease your signal-to-noise ratio on samples with high auto-fluorescence Narrow Excitation Cy5 The emission at these wavelengths will be better detected by camera than by the unaided eye. Only a small percentage of humans can detect these wavelengths. This set has been discontinued. Please see replacement set Rhodamine X / Resorufin This set replaces discontinued sets: 31046, 41010, Narrow Excitation (Hg) DsRed Recommended for use with mercury lamps only. by special request Li-Cor for IR Dye 800 This set requires a detector with a good QE in the nm range. This set has been discontinued. Please see replacement set HQ405/20x Exciter (EX) $ dcxr Dichroic (BS) $ HQ430LP Emitter (EM) $ Total...$ HQ545/30x Exciter (EX) $ Q565LP Dichroic (BS) $ HQ572LP Emitter (EM) $ Total...$ HQ640/30x Exciter (EX) $ Q660LP Dichroic (BS) $ HQ700/75m Emitter (EM) $ Total... HQ570/20x Exciter (EX) $ Q585LP Dichroic (BS) $ HQ620/60m Emitter (EM) $ Total...$ HQ546/11x Exciter (EX) $ Q560LP Dichroic (BS) $ HQ605/75m Emitter (EM) $ Total...$ HQ740/35x Exciter (EX) $ dcxr Dichroic (BS) $ HQ780LP Emitter (EM) $ Total... J u n e

31 C h r o m a Te c h n o l o g y Co r p Series - HQ Filter Sets This filter series was designed to increase brightness without a corresponding increase in noise. By increasing the number of interference cavities in both the excitation and emission filters, we have created filters with steeper passbands. This allowed wider excitation and emission filters which were spectrally closer together than the Standard series. Wider and steeper filters provide more excitation and capture more fluorescence, resulting in greater signal to noise. This unique design also allows for the excitation/dichroic/emissions to be placed closer together for those dyes with a very short Stoke s shift Spc. Yellow set for viewing TRITC, Cy3, Texas Red HQ560/40x Exciter (EX) $ Q590LP Dichroic (BS) $ HQ620/60m Emitter (EM) $ Total...$ Spectrum Gold For use with mercury lamps only. This is our equivalent for Spectrum Gold. The official Spectrum Gold set must be obtained through Vysis. This set has been discontinued. Please see replacement set HQ546/11x Exciter (EX) $ Q555LP Dichroic (BS) $ HQ575/30m Emitter (EM) $ Total HcRed1 HQ575/50x Exciter (EX) $ Q610LP Dichroic (BS) $ HQ640/50m Emitter (EM) $ Total...$ FM1-43/Chlorophyll This set replaces discontinued sets: 31017, 31017a HQ480/40x Exciter (EX) $ DCXT Dichroic (BS) $ HQ600lp Emitter (EM) $ Total...$ C h r o m a T e c h n o l o g y C o r p

32 P r i c e Li s t o f Fi l t e r s Series - Reef Coral Fluorescent Protein Sets AmCyan1 by special request ZsGreen1 by special request ZsYellow1 by special request AsRed2 by special request. HQ440/40x Exciter (EX) $ Q470LP Dichroic (BS) $ HQ500/40m Emitter (EM) $ Total...$ HQ470/30x Exciter (EX) $ Q495LP Dichroic (BS) $ HQ520/40m Emitter (EM) $ Total...$ HQ500/35x Exciter (EX) $ Q525LP Dichroic (BS) $ HQ550/40m Emitter (EM) $ Total...$ HQ540/40x Exciter (EX) $ Q570LP Dichroic (BS) $ HQ620/60m Emitter (EM) $ Total...$ DsRed2 HQ540/40x Exciter (EX) $ Q570LP Dichroic (BS) $ HQ600/50m Emitter (EM) $ Total...$ J u n e

33 C h r o m a Te c h n o l o g y Co r p Series - ET Sputtered Single Band Filter Sets This series features Chroma s Modified Magnetron sputter coatings (ET) which achieve the highest transmission levels for interference filters. Like the series, these filters also have a very steep transition from peak transmission to high optical density to best exploit the spectral properties of modern fluorochromes with short Stokes shifts. These filters offer the greatest hardness and durability against environmental factors such as high heat and humidity, and will last much longer than soft-coatings. Additionally, use of our ET filters assures image registration between filter sets in imaging systems which are otherwise in perfect register ET - DAPI AT350/50x Exciter (EX) $ T400LP Dichroic (BS) $ ET460/50m Emitter (EM) $ Total...$ ET - CFP Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample ET - GFP (FITC/Cy2) Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample ET - EYFP Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample ET - CY3 Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample ET - DSRed (TRITC/Cy3) Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample. This set replaces discontinued set: 41002a ET436/20x Exciter (EX) $ T455LP Dichroic (BS) $ ET480/40m Emitter (EM) $ Total...$ ET470/40x Exciter (EX) $ T495lpxr Dichroic (BS) $ ET525/50m Emitter (EM) $ Total...$ ET500/20x Exciter (EX) $ T515LP Dichroic (BS) $ ET535/30m Emitter (EM) $ Total...$ ET545/25x Exciter (EX) $ T565lpxr Dichroic (BS) $ ET605/70m Emitter (EM) $ Total...$ ET545/30x Exciter (EX) $ T570LP Dichroic (BS) $ ET620/60m Emitter (EM) $ Total...$ C h r o m a T e c h n o l o g y C o r p

34 P r i c e Li s t o f Fi l t e r s Series - ET Sputtered Single Band Filter Sets This series features Chroma s Modified Magnetron sputter coatings (ET) which achieve the highest transmission levels for interference filters. Like the series, these filters also have a very steep transition from peak transmission to high optical density to best exploit the spectral properties of modern fluorochromes with short Stokes shifts. These filters offer the greatest hardness and durability against environmental factors such as high heat and humidity, and will last much longer than soft-coatings. Additionally, use of our ET filters assures image registration between filter sets in imaging systems which are otherwise in perfect register ET - Cy5 Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample ET - Cy7 Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample. This set replaces discontinued set: ET - mcherry, Texas Red Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample ET - Cy5 Narrow Excitation The narrow-band excitation provided by this filter set is useful in applications where fluorochromes such as Texas Red or AlexaFluor 594 are also present in the same sample. This set will minimize bleedthrough of these fluorochromes. This set replaces discontinued set: ET620/60x Exciter (EX) $ T660LPXR Dichroic (BS) $ ET700/75m Emitter (EM) $ Total...$ ET710/75x Exciter (EX) $ T760lpxr Dichroic (BS) $ ET810/90m Emitter (EM) $ Total...$ ET560/40x Exciter (EX) $ T585lpxr Dichroic (BS) $ ET630/75m Emitter (EM) $ Total...$ ET640/30x Exciter (EX) $ T660LPXR Dichroic (BS) $ ET690/50m Emitter (EM) $ Total...$ ET - R&B Phycoerythrin/mOrange/mKO Recommended for use with mercury lamps or metalhalide lamps only. Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample. This set replaces discontinued sets: 41003, ET546/10x Exciter (EX) $ T560LPXR Dichroic (BS) $ ET585/40m Emitter (EM) $ Total...$ J u n e

35 C h r o m a Te c h n o l o g y Co r p Series - ET Sputtered Single Band Filter Sets This series features Chroma s Modified Magnetron sputter coatings (ET) which achieve the highest transmission levels for interference filters. Like the series, these filters also have a very steep transition from peak transmission to high optical density to best exploit the spectral properties of modern fluorochromes with short Stokes shifts. These filters offer the greatest hardness and durability against environmental factors such as high heat and humidity, and will last much longer than soft-coatings. Additionally, use of our ET filters assures image registration between filter sets in imaging systems which are otherwise in perfect register ET - FITC/Alexa Fluor 488/Fluo3/Oregon Green Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample ET - FITC/GFP LP Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample ET - EBFP2/Coumarin/Attenuated DAPI Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample. This set replaces discontinued sets: 31016, 31021, 31047v ET - YFP Longpass Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample. This set replaces discontinued set: ET - Indocyanine Green Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample. This set replaces discontinued set: ET - Li_Cor for IR dye 800 Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample. This set replaces discontinued set: ET480/40x Exciter (EX) $ T510LPXRXT Dichroic (BS) $ ET535/50m Emitter (EM) $ Total...$ ET480/40x Exciter (EX) $ T510LPXRXT Dichroic (BS) $ ET510lp Emitter (EM) $ Total...$ ET405/20x Exciter (EX) $ T425lpxr Dichroic (BS) $ ET460/50m Emitter (EM) $ Total...$ ET500/20x Exciter (EX) $ T515LP Dichroic (BS) $ ET520LP Emitter (EM) $ Total...$ ET775/50x Exciter (EX) $ T810lpxr Dichroic (BS) $ ET845/55m Emitter (EM) $ Total...$ ET740/40x Exciter (EX) $ T770LPXR Dichroic (BS) $ ET780LP Emitter (EM) $ Total...$ C h r o m a T e c h n o l o g y C o r p

36 P r i c e Li s t o f Fi l t e r s Series - ET Sputtered Single Band Filter Sets This series features Chroma s Modified Magnetron sputter coatings (ET) which achieve the highest transmission levels for interference filters. Like the series, these filters also have a very steep transition from peak transmission to high optical density to best exploit the spectral properties of modern fluorochromes with short Stokes shifts. These filters offer the greatest hardness and durability against environmental factors such as high heat and humidity, and will last much longer than soft-coatings. Additionally, use of our ET filters assures image registration between filter sets in imaging systems which are otherwise in perfect register ET - CFP/YFP FRET Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample. This set replaces discontinued set: ET436/20x Exciter (EX) $ T455LP Dichroic (BS) $ ET535/30m Emitter (EM) $ Total...$ J u n e

37 C h r o m a Te c h n o l o g y Co r p Series - Dual Band Filter Sets The dual-band filter sets were designed for simultaneous viewing of two fluorochromes. Each excitation filter contains two passbands for simultaneous excitation. Concurrently, each emission filter contains two passbands for simultaneous viewing. The polychroic beamsplitters contain complementary reflection and transmission bands specific to the excitation and emission filters. These sets were originally designed for FISH (fluorescence in situ hybridization). During their history, their performance has been relied upon in many other applications DAPI/FITC The UV exciter in this set is centered around 400 nm. DAPI will be considerably brighter than Hoechst, which will not be efficiently excited. This set will not excite AMCA DAPI/TRITC The UV exciter in this set is centered around 400 nm. DAPI will be considerably brighter than Hoechst, which will not be efficiently excited. This set uses the 546 nm line of the mercury lamp for TRITC excitation. It will not excite AMCA DAPI/Propidium Iodide The UV exciter in this set is centered around 400 nm. DAPI will be considerably brighter than Hoechst. It will not excite AMCA. This is the same set as This set has been discontinued. Please see replacement set DAPI/Texas Red or DAPI/Propidium Iodide The DAPI exciter in this set is centered around 400 nm. It will not efficiently excite Hoechst dyes. This set replaces discontinued set: v2 FITC/TRITC This set utilizes the 546 nm line in the mercury lamp for TRITC excitation. by special request. Also refer to replacement set FITC/Propidium Iodide This is the same filter set as It is designed to attenuate the typically bright PI emission and balance it with the emission of FITC. This set has been discontinued. Please see replacement set x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ v2x Exciter (EX) $ v2bs Dichroic (BS) $ v2m Emitter (EM) $ Total...$ x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total C h r o m a T e c h n o l o g y C o r p

38 P r i c e Li s t o f Fi l t e r s Series - Dual Band Filter Sets The dual-band filter sets were designed for simultaneous viewing of two fluorochromes. Each excitation filter contains two passbands for simultaneous excitation. Concurrently, each emission filter contains two passbands for simultaneous viewing. The polychroic beamsplitters contain complementary reflection and transmission bands specific to the excitation and emission filters. These sets were originally designed for FISH (fluorescence in situ hybridization). During their history, their performance has been relied upon in many other applications FITC/Texas Red or FITC/Propidium Iodide This is the same filter set as This set replaces discontinued set: Cy3 /Cy5 This set utilizes the 546 nm line for Cy3 excitation. Useful for most orange-emitting dyes such as TRITC. Cy5 emission cannot be seen by eye. This set will require an IR-sensitive color CCD camera. by special request FITC/Cy5 Cy5 emission cannot be seen by eye. This set will require an IR-sensitive color CCD camera. by special request FITC/Cy3 This set does not utilize the 546 nm mercury line. It is designed to balance the Cy3 and FITC signals in duallabeled samples DAPI/Cy3 This set attenuates the Cy3 signal (or other orange-emitting dye) when compared to the set by utilizing a narrower emission filter. Both utilize the 546 nm mercury line. by special request DAPI/GFP This is the same filter set as by special request x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ J u n e

39 C h r o m a Te c h n o l o g y Co r p Series - Dual Band Filter Sets The dual-band filter sets were designed for simultaneous viewing of two fluorochromes. Each excitation filter contains two passbands for simultaneous excitation. Concurrently, each emission filter contains two passbands for simultaneous viewing. The polychroic beamsplitters contain complementary reflection and transmission bands specific to the excitation and emission filters. These sets were originally designed for FISH (fluorescence in situ hybridization). During their history, their performance has been relied upon in many other applications DAPI/Cy5 by special request FITC/DiI This set is designed to work with the 546 nm line of the mercury arc lamp. It is not recommended for other light sources. by special request Lucifer Yellow/Rhodamine or DiI This set is designed to work with a mercury lamp. It is not recommended for other light sources. by special request CFP/YFP CFP bleedthrough into the YFP emission channel makes it difficult to clearly separate these two fluorochromes with a dual-band set. For better results use single-band exciters. by special request. Also refer to replacement set x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ ECFP/DsRed 51018x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ EGFP/DsRed by special request. Also refer to replacement set x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ C h r o m a T e c h n o l o g y C o r p

40 P r i c e Li s t o f Fi l t e r s Series - Dual Band Filter Sets The dual-band filter sets were designed for simultaneous viewing of two fluorochromes. Each excitation filter contains two passbands for simultaneous excitation. Concurrently, each emission filter contains two passbands for simultaneous viewing. The polychroic beamsplitters contain complementary reflection and transmission bands specific to the excitation and emission filters. These sets were originally designed for FISH (fluorescence in situ hybridization). During their history, their performance has been relied upon in many other applications GFP/mRFP x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ J u n e

41 C h r o m a Te c h n o l o g y Co r p Series - Dual Band Filter Sets With Single Band Excitation Filters 52004v2 FITC/TRITC W/Single Band Exciters by special request. Also refer to replacement set FITC/PI W/Single Band Exciters by special request. Also refer to replacement set FITC/Texas Red W/Single Band Exciters by special request. Also refer to replacement set CFP/YFP with Single-Band Exciters by special request. Also refer to replacement set CFP/DsRed with Single-Band Exciters by special request. S484/15x Exciter (EX) $ S555/25x Exciter (EX) $ v2bs Dichroic (BS) $ v2m Emitter (EM) $ Total...$ S492/18x Exciter (EX) $ S572/23x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ S492/18x Exciter (EX) $ S572/23x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ S436/10x Exciter (EX) $ S500/20x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ S430/25x Exciter (EX) $ S545/30x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ C h r o m a T e c h n o l o g y C o r p

42 P r i c e Li s t o f Fi l t e r s Series - Dual Band Filter Sets With Single Band Excitation Filters EGFP/DsRed with Single-Band Exciters by special request. Also refer to replacement set. S480/25x Exciter (EX) $ S565/25x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ J u n e

43 C h r o m a Te c h n o l o g y Co r p Series - ET Sputtered Dual Band Filter Sets and Dual Band Sets With Single Band Exciters This series of dual-band filter sets feature our high performance ET sputter coatings. Emission filters have two passbands specific for a particular pair of fluorochromes. The sets with dual-band excitation filters allow for simultaneous visualization of two fluorochromes as often required for applications such as FISH or screening assays. The sets with two single-band excitation filters allow for selective excitation, but require the use of excitation filter wheels or sliders. NOTE: sets with multi-band excitation filters are not appropriate for imaging with a monochrome camera FITC/TRITC - ET Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample. This set replaces discontinued sets: 51004v2, CFP/YFP - ET Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample. This set replaces discontinued set: GFP/mCherry (or FITC/TxRed) - ET Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample FITC/TRITC with single band exciters - ET Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample. This set replaces discontinued set: 52004v CFP/YFP with single band exciters - ET Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample. This set replaces discontinued set: x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ ET470/40x Exciter (EX) $ ET548/10x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ ET430/24x Exciter (EX) $ ET500/20x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ C h r o m a T e c h n o l o g y C o r p

44 P r i c e Li s t o f Fi l t e r s Series - ET Sputtered Dual Band Filter Sets and Dual Band Sets With Single Band Exciters This series of dual-band filter sets feature our high performance ET sputter coatings. Emission filters have two passbands specific for a particular pair of fluorochromes. The sets with dual-band excitation filters allow for simultaneous visualization of two fluorochromes as often required for applications such as FISH or screening assays. The sets with two single-band excitation filters allow for selective excitation, but require the use of excitation filter wheels or sliders. NOTE: sets with multi-band excitation filters are not appropriate for imaging with a monochrome camera GFP/mCherry (or FITC/TxRed) with single band exciters - ET Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample. This set replaces discontinued sets: 52005, ET470/40x Exciter (EX) $ ET572/35x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ J u n e

45 C h r o m a Te c h n o l o g y Co r p Series - Triple Band Filter Sets The triple band filter sets were designed for simultaneous viewing of three fluorochromes. Each excitation filter contains three passbands for simultaneous excitation. Concurrently each emission filter contains three passbands for simultaneous viewing. The polychroic beamsplitters contain complementary reflection and transmission bands specific to the excitation and emission filters. Like the dual band series, these sets were originally designed for FISH (fluorescence in situ hybridization). Also like the dual band sets these filter sets have shown great practicality for other applications v2 DAPI/FITC/TRITC or Cy3 The UV exciter in this set is centered around 400 nm. DAPI will be considerably brighter than Hoechst when using this set. This set will not excite AMCA. by special request. Also refer to replacement set DAPI/FITC/Propidium Iodide The UV exciter in this set is centered around 400 nm. DAPI will be considerably brighter than Hoechst. It will not excite AMCA. This is the same set as and is designed to attenuate the typically bright PI signal. by special request. Also refer to replacement set DAPI/FITC/Texas Red This is the same set as by special request. Also refer to replacement set DAPI/FITC/Cy5 The UV exciter in this set is centered around 400 nm. DAPI will be considerably brighter than Hoechst. This set will not excite AMCA. Cy5 emission cannot be seen by eye and the use of this set requires an IR-sensitive color CCD. by special request DAPI/Cy3 /Cy5 The UV exciter in this set is centered around 400 nm. DAPI will be considerably brighter than Hoechst. This set will not excite AMCA. Cy5 emission cannot be seen by eye and the use of this set requires an IR-sensitive color CCD. by special request v2x Exciter (EX) $ v2bs Dichroic (BS) $ v2m Emitter (EM) $ Total...$ x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ C h r o m a T e c h n o l o g y C o r p

46 P r i c e Li s t o f Fi l t e r s Series - Triple Band Filter Sets The triple band filter sets were designed for simultaneous viewing of three fluorochromes. Each excitation filter contains three passbands for simultaneous excitation. Concurrently each emission filter contains three passbands for simultaneous viewing. The polychroic beamsplitters contain complementary reflection and transmission bands specific to the excitation and emission filters. Like the dual band series, these sets were originally designed for FISH (fluorescence in situ hybridization). Also like the dual band sets these filter sets have shown great practicality for other applications FITC/Cy3 /Cy5 Cy5 emission cannot be seen by eye and the use of this set requires an IR-sensitive color CCD. by special request FITC/Texas Red /Cy5 Cy5 emission cannot be seen by eye and the use of this set requires an IR-sensitive color CCD. by special request CFP/YFP/DsRed by special request. Also refer to replacement set CFP/YFP/Cy5 Cy5 emission cannot be seen by eye and the use of this set requires an IR-sensitive color CCD. by special request DAPI/FITC/Cy3 This set is designed to attenuate the Cy3 signal to better balance it with FITC. by special request. Also refer to replacement set x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ J u n e

47 C h r o m a Te c h n o l o g y Co r p Series - Triple Band Filter Sets With Single Band Excitation Filters 62000v3 DAPI/FITC/TRITC or Cy3 with single band exciters by special request. Also refer to replacement set DAPI/FITC/Propidium Iodide by special request. Also refer to replacement set v2 DAPI/FITC/Texas Red w/single-band exciters by special request. Also refer to replacement set AT350/50x Exciter (EX) $ S484/15x Exciter (EX) $ S555/25x Exciter (EX) $ v2bs Dichroic (BS) $ v2m Emitter (EM) $ Total...$ AT350/50x Exciter (EX) $ S492/18x Exciter (EX) $ S572/23x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ AT350/50x Exciter (EX) $ S492/18x Exciter (EX) $ S572/23x Exciter (EX) $ BS Dichroic (BS) $ m Emitter (EM) $ Total...$ C h r o m a T e c h n o l o g y C o r p

48 P r i c e Li s t o f Fi l t e r s Series - ET Sputtered Triple Band Filter Sets and Triple Band Sets with Single Band Exciters This series of triple-band filter sets feature our high performance ET sputter coatings. Emission filters have three passbands specific for a particular trio of fluorochromes. The sets with triple-band excitation filters allow for simultaneous visualization of three fluorochromes as often required for applications such as FISH or screening assays. The sets with three single-band excitation filters allow for selective excitation, but require the use of excitation filter wheels or sliders. NOTE: sets with multi-band excitation filters are not appropriate for imaging with a monochrome camera DAPI/FITC/TRITC - ET Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample. This set replaces discontinued set: 61000v DAPI/FITC/Texas Red - ET Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample. This set replaces discontinued sets: 61001, CFP/YFP/mCherry - ET Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample. This set replaces discontinued set: Dapi/FITC/TRITC with single band exciters - ET Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample. This set replaces discontinued set: 62000v DAPI/FITC/Texas Red with single band exciters - ET Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample. This set replaces discontinued sets: 62001, 62002v x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ ET402/15x Exciter (EX) $ ET485/20x Exciter (EX) $ ET550/15x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ ET402/15x Exciter (EX) $ ET490/20x Exciter (EX) $ ET572/35x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ J u n e

49 C h r o m a Te c h n o l o g y Co r p Series - ET Sputtered Triple Band Filter Sets and Triple Band Sets with Single Band Exciters This series of triple-band filter sets feature our high performance ET sputter coatings. Emission filters have three passbands specific for a particular trio of fluorochromes. The sets with triple-band excitation filters allow for simultaneous visualization of three fluorochromes as often required for applications such as FISH or screening assays. The sets with three single-band excitation filters allow for selective excitation, but require the use of excitation filter wheels or sliders. NOTE: sets with multi-band excitation filters are not appropriate for imaging with a monochrome camera CFP/YFP/mCherry with single band exciters - ET Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample. ET430/24x Exciter (EX) $ ET500/20x Exciter (EX) $ ET577/25x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ C h r o m a T e c h n o l o g y C o r p

50 P r i c e Li s t o f Fi l t e r s Series - Dual Excitation and Dual Emission Dye Filter Sets This series of filters was designed to allow either multiple excitation or multiple emission of a fluorochrome. By using filters mounted in either a wheel or slider, this design allows for ratiometry utilizing either two excitation wavelengths or two emissions. This capability obviously requires specialized hardware and software on the part of the investigator av2 Fura 2 with wide band emission filter. See set# This set has been discontinued. Please see replacement set Total v2 Fura 2. See set# This set has been discontinued. Please see replacement set Total a BCECF with wider D440 filter for more balanced excitation This is the recommended filter set for ratiometric analysis of BCECF. This set has been discontinued. Please see replacement set a INDO - No emission beamsplitter This set is for ratiometric analysis of INDO with an emission filter wheel. For simultaneous imaging with an emission-splitting system, use the set. This set has been discontinued. Please see replacement set Fura Red This set is for ratiometric imaging of Fura Red. For non-ratiometric imaging of this dye, use the set. by special request. D440/20x Exciter (EX) $ D495/10x Exciter (EX) $ dcxr Dichroic (BS) $ D535/25m Emitter (EM) $ Total... D365/10x Exciter (EX) $ DCLP Dichroic (BS) $ D405/30m Emitter (EM) $ D485/25m Emitter (EM) $ Total... D440/10x Exciter (EX) $ D490/10x Exciter (EX) $ dcxr Dichroic (BS) $ D660/50m Emitter (EM) $ Total...$ J u n e

51 C h r o m a Te c h n o l o g y Co r p Series - Dual Excitation and Dual Emission Dye Filter Sets This series of filters was designed to allow either multiple excitation or multiple emission of a fluorochrome. By using filters mounted in either a wheel or slider, this design allows for ratiometry utilizing either two excitation wavelengths or two emissions. This capability obviously requires specialized hardware and software on the part of the investigator SNAFL D510/25x Exciter (EX) $ HQ555/28x Exciter (EX) $ dcxr Dichroic (BS) $ D620/40m Emitter (EM) $ Total...$ a SNARF ( with 515nm excitation) This set is for simultaneous imaging using an emissionsplitting system. If you intend to image sequentially with a filter wheel, do not order the 610DCLP and subtract $ from the set price b SNARF ( with 534nm excitation) This set is for simultaneous imaging using an emissionsplitting system. If you intend to image sequentially with a filter wheel, do not order the 610DCLP and subtract $ from the set price c SNARF ( with 488nm excitation) This set is for simultaneous imaging using an emissionsplitting system. If you intend to image sequentially with a filter wheel, do not order the 610DCLP and subtract $ from the set price. D515/30x Exciter (EX) $ DCLP Dichroic (BS) $ Q610LP Dichroic (BS) $ D580/25m Emitter (EM) $ D640/25m Emitter (EM) $ Total...$ D535/25x Exciter (EX) $ DCLP Dichroic (BS) $ DCLP Dichroic (BS) $ D580/25m Emitter (EM) $ D640/25m Emitter (EM) $ Total...$ D485/25x Exciter (EX) $ DCLP Dichroic (BS) $ DCLP Dichroic (BS) $ D580/25m Emitter (EM) $ D640/25m Emitter (EM) $ Total...$ C h r o m a T e c h n o l o g y C o r p

52 P r i c e Li s t o f Fi l t e r s Series - Dual Excitation and Dual Emission Dye Filter Sets This series of filters was designed to allow either multiple excitation or multiple emission of a fluorochrome. By using filters mounted in either a wheel or slider, this design allows for ratiometry utilizing either two excitation wavelengths or two emissions. This capability obviously requires specialized hardware and software on the part of the investigator Di 4 and 8 ANNEPS This set is for ratiometric imaging of either Di-4- or Di-8- ANNEPS. For non-ratiometric imaging of these dyes, use set a Cameleons 2 - No Emission Beamsplitter This set is for ratiometric analysis of Cameleons with an emission filter wheel. For simultaneous imaging with an emission-splitting system, use the set. This set has been discontinued. Please see replacement set a Beta Lactamase Ratiometric This set is for ratiometric analysis of Beta Lactamase with an emission filter wheel. For simultaneous imaging with an emission-splitting system, use the set. For non-ratiometric analysis, use the set. This set has been discontinued. Please see replacement set a JC1 This set is for ratiometric imaging of JC1 using an emission filter wheel. For simultaneous imaging using an emission-splitting system, use the set. This set has been discontinued. Please see replacement set D440/20x Exciter (EX) $ D530/20x Exciter (EX) $ dcxr Dichroic (BS) $ D605/54m Emitter (EM) $ Total...$ D436/20x Exciter (EX) $ DCLP Dichroic (BS) $ D485/40m Emitter (EM) $ D535/30m Emitter (EM) $ Total... HQ405/20x Exciter (EX) $ dcxr Dichroic (BS) $ HQ460/40m Emitter (EM) $ HQ530/30m Emitter (EM) $ Total... D480/30x Exciter (EX) $ DCLP Dichroic (BS) $ D535/40m Emitter (EM) $ E580LP Emitter (EM) $ Total ph Sensitive GFP D410/30x Exciter (EX) $ D470/20x Exciter (EX) $ dcxr Dichroic (BS) $ HQ535/48m Emitter (EM) $ Total...$ J u n e

53 C h r o m a Te c h n o l o g y Co r p Series - Dual Excitation and Dual Emission Dye Filter Sets This series of filters was designed to allow either multiple excitation or multiple emission of a fluorochrome. By using filters mounted in either a wheel or slider, this design allows for ratiometry utilizing either two excitation wavelengths or two emissions. This capability obviously requires specialized hardware and software on the part of the investigator Pericam D415/30x Exciter (EX) $ HQ485/15x Exciter (EX) $ dcxr Dichroic (BS) $ HQ535/48m Emitter (EM) $ Total...$ BCECF with wider D440 filter for more balanced excitation This is the recommended filter set for ratiometric analysis of BCECF. This set replaces discontinued set: 71001a INDO - No emission beamsplitter This set is for ratiometric analysis of INDO with an emission filter wheel. This set replaces discontinued set: 71002a Cameleons 2 - No Emission Beamsplitter This set is for ratiometric analysis of Cameleons with an emission filter wheel. This set replaces discontinued set: 71007a Beta lactamase Ratiometric This set is for ratiometric analysis of coumarin and fluorescein in Beta Lactamase assays with an emission filter wheel. This set replaces discontinued set: 71008a D440/20x Exciter (EX) $ D495/10x Exciter (EX) $ dcxr Dichroic (BS) $ D535/25m Emitter (EM) $ Total...$ D365/10x Exciter (EX) $ DCLP Dichroic (BS) $ D405/30m Emitter (EM) $ D485/25m Emitter (EM) $ Total...$ D436/20x Exciter (EX) $ DCLP Dichroic (BS) $ D485/40m Emitter (EM) $ D535/30m Emitter (EM) $ Total...$ HQ405/20x Exciter (EX) $ dcxr Dichroic (BS) $ HQ460/40m Emitter (EM) $ HQ530/30m Emitter (EM) $ Total...$ C h r o m a T e c h n o l o g y C o r p

54 P r i c e Li s t o f Fi l t e r s Series - Dual Excitation and Dual Emission Dye Filter Sets This series of filters was designed to allow either multiple excitation or multiple emission of a fluorochrome. By using filters mounted in either a wheel or slider, this design allows for ratiometry utilizing either two excitation wavelengths or two emissions. This capability obviously requires specialized hardware and software on the part of the investigator JC1 This set is for ratiometric imaging of JC1 using an emission filter wheel. This set replaces discontinued set: 71010a D480/30x Exciter (EX) $ DCXT Dichroic (BS) $ D535/40m Emitter (EM) $ E580LP Emitter (EM) $ Total...$ J u n e

55 C h r o m a Te c h n o l o g y Co r p Series - ET Sputtered Dual Excitation and Dual Emission Dye Filter Sets ET FURA2 Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample. This set replaces discontinued sets: 71000v2, 71000av2, ET340x Exciter (EX) $ ET380x Exciter (EX) $ T400LP Dichroic (BS) $ ET510/80m Emitter (EM) $ Total...$ C h r o m a T e c h n o l o g y C o r p

56 P r i c e Li s t o f Fi l t e r s Series - Multi-Band Filter Sets with Single Emitters and Multiple Exciters Originally designed for Comparative Genomic Hybridization the filter sets in this series, like those in the 51 and 61 series have become important in a wide variety of applications. The factors common to each of the 80 series filter sets is the use of a multiband beamsplitter and emission filter combination which remain stable in the body of the fluorescence microscope. The use of a stable beamsplitter and emission filter guarantees registration of the various images. The single, and sometimes multi-band excitation filters are mounted in a filter wheel or a filter slider. By changing excitation filters one seeks to excite each fluorochrome in succession, capturing a single image and combining those images with the help of imaging software. There can be some bleeding of one fluorochrome into the image of a shorter wavelength fluorochrome. This is sometimes inevitable, as is the case with Propidium Iodide and FITC v2 Pinkel #1 Filter Set Note that in multi-labeled samples the AT350/50x exciter may excite multiple fluorochromes resulting in potential crosstalk when the triple-emission filter is used v2 DAPI/FITC/TRITC or Cy3 This set is spectrally equivalent to the set but with a multiband emission filter. Note that in multi-labeled samples the AT350/50x exciter may excite multiple fluorochromes resulting in potential crosstalk when the triple-emission filter is used Supplementary Emission Filters for Filter Set Using these in lieu of the triple-emission filter will result in the inability to visualize the samples by eye when using an external filter wheel. by special request x Exciter (EX) $ AT350/50x Exciter (EX) $ S403/12x Exciter (EX) $ S492/18x Exciter (EX) $ S572/23x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ x Exciter (EX) $ x Exciter (EX) $ AT350/50x Exciter (EX) $ S395/10x Exciter (EX) $ S484/15x Exciter (EX) $ S555/25x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ S457/17m Emitter (EM) $ S517/30m Emitter (EM) $ S605/40m Emitter (EM) $ Total...$ J u n e

57 C h r o m a Te c h n o l o g y Co r p Series - Multi-Band Filter Sets with Single Emitters and Multiple Exciters Originally designed for Comparative Genomic Hybridization the filter sets in this series, like those in the 51 and 61 series have become important in a wide variety of applications. The factors common to each of the 80 series filter sets is the use of a multiband beamsplitter and emission filter combination which remain stable in the body of the fluorescence microscope. The use of a stable beamsplitter and emission filter guarantees registration of the various images. The single, and sometimes multi-band excitation filters are mounted in a filter wheel or a filter slider. By changing excitation filters one seeks to excite each fluorochrome in succession, capturing a single image and combining those images with the help of imaging software. There can be some bleeding of one fluorochrome into the image of a shorter wavelength fluorochrome. This is sometimes inevitable, as is the case with Propidium Iodide and FITC v2 DAPI/FITC/Texas Red This set is spectrally equivalent to the set but with a multiband emission filter. Note that in multi-labeled samples the AT350/50x exciter may excite multiple fluorochromes resulting in potential crosstalk with the triple-emission filter is used Supplementary Emission Filters for Filter Set Using these in lieu of the triple-emission filter will result in the inability to visualize the samples by eye when using an external filter wheel. by special request v2 DAPI/FITC/TRITC/Cy5 Quad This set is spectrally equivalent to the set but with a quad emitter. Note that in multi-labeled samples the AT350/50x exciter may excite multiple fluorochromes resulting in potential crosstalk when the quad emission filter is used x Exciter (EX) $ x Exciter (EX) $ AT350/50x Exciter (EX) $ S403/12x Exciter (EX) $ S492/18x Exciter (EX) $ S572/23x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ S460/36m Emitter (EM) $ S530/35m Emitter (EM) $ S630/60m Emitter (EM) $ Total...$ AT350/50x Exciter (EX) $ S403/12x Exciter (EX) $ S490/20x Exciter (EX) $ S555/28x Exciter (EX) $ S635/20x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ C h r o m a T e c h n o l o g y C o r p

58 P r i c e Li s t o f Fi l t e r s Series - Multi-Band Filter Sets with Single Emitters and Single Exciters 86000v2 Sedat Quad AT350/50x Exciter (EX) $ S403/12x Exciter (EX) $ S490/20x Exciter (EX) $ S555/28x Exciter (EX) $ S635/20x Exciter (EX) $ bs Dichroic (BS) $ S457/50m Emitter (EM) $ S528/38m Emitter (EM) $ S617/73m Emitter (EM) $ S685/40m Emitter (EM) $ Total...$ JP3 - EGFP and YFP EGFP and EYFP are extremely difficult to separate in any imaging device. Please see Hagting, A., Jackman, M., Simpson, K. and Pines, J. (1999) The translocation of cyclin B1 to the nucleus at prophase requires a phosphorylation-dependent nuclear import signal. Current Biology, 9, for more details. by special request v1 JP4 - CFP and YFP Recommended for use with mercury light source. by special request. Also refer to replacement set S460/20x Exciter (EX) $ S523/20x Exciter (EX) $ bs Dichroic (BS) $ S500/22m Emitter (EM) $ S568/50m Emitter (EM) $ Total...$ S436/10x Exciter (EX) $ S500/20x Exciter (EX) $ v1bs Dichroic (BS) $ S470/30m Emitter (EM) $ S535/30m Emitter (EM) $ Total...$ J u n e

59 C h r o m a Te c h n o l o g y Co r p Series - Multi-Band Filter Sets with Single Emitters and Single Exciters 86002v2 JP4 - CFP and YFP Recommended for use with either xenon or mercury light sources. by special request. Also refer to replacement set BFP and EGFP by special request v1 JP5 - UVGFP (Sapphire)/CFP/YFP In this set, both UVGFP and YFP share the S535/30m emission filter. Recommended for use with mercury lamps only. by special request v2 JP5 - UVGFP (Sapphire)/CFP/YFP This set is the same as 86004v1 except that it can be used with either mercury or xenon light sources. by special request. S430/25x Exciter (EX) $ S500/20x Exciter (EX) $ v2bs Dichroic (BS) $ S470/30m Emitter (EM) $ S535/30m Emitter (EM) $ Total...$ S380/30x Exciter (EX) $ S485/25x Exciter (EX) $ bs Dichroic (BS) $ S445/40m Emitter (EM) $ S535/50m Emitter (EM) $ Total...$ S395/40x Exciter (EX) $ S436/10x Exciter (EX) $ S500/20x Exciter (EX) $ v1bs Dichroic (BS) $ S470/30m Emitter (EM) $ S535/30m Emitter (EM) $ Total...$ S395/40x Exciter (EX) $ S430/25x Exciter (EX) $ S500/20x Exciter (EX) $ v2bs Dichroic (BS) $ S470/30m Emitter (EM) $ S535/30m Emitter (EM) $ Total...$ C h r o m a T e c h n o l o g y C o r p

60 P r i c e Li s t o f Fi l t e r s Series - Multi-Band Filter Sets with Single Emitters and Single Exciters BFP and YFP by special request CFP/YFP/DsRed Recommended for use with mercury lamps only. by special request. Also refer to replacement set EGFP/DsRed by special request. Also refer to replacement set S380/30x Exciter (EX) $ S500/20x Exciter (EX) $ bs Dichroic (BS) $ S465/30m Emitter (EM) $ S535/30m Emitter (EM) $ Total...$ S436/10x Exciter (EX) $ S492/18x Exciter (EX) $ S580/20x Exciter (EX) $ bs Dichroic (BS) $ S465/30m Emitter (EM) $ S535/30m Emitter (EM) $ S630/60m Emitter (EM) $ Total...$ S480/20x Exciter (EX) $ S565/25x Exciter (EX) $ bs Dichroic (BS) $ S525/40m Emitter (EM) $ S620/60m Emitter (EM) $ Total...$ J u n e

61 C h r o m a Te c h n o l o g y Co r p Series - Multi-Band Filter Sets with Single Emitters and Single Exciters 86008v1 CFP/YFP and Cy5 v1 Recommended for use with mercury lamps only. by special request. S436/10x Exciter (EX) $ S510/20x Exciter (EX) $ S622/36x Exciter (EX) $ bs Dichroic (BS) $ S470/30m Emitter (EM) $ S560/50m Emitter (EM) $ S700/75m Emitter (EM) $ Total...$ v2 CFP/YFP and Cy5 v2 S430/25x Exciter (EX) $ S510/20x Exciter (EX) $ S622/36x Exciter (EX) $ bs Dichroic (BS) $ S465/30m Emitter (EM) $ S550/50m Emitter (EM) $ S700/75m Emitter (EM) $ Total...$ BFP/GFP and DsRed by special request. D380/30x Exciter (EX) $ S484/15x Exciter (EX) $ S555/25x Exciter (EX) $ bs Dichroic (BS) $ S450/30m Emitter (EM) $ S520/30m Emitter (EM) $ S620/60m Emitter (EM) $ Total...$ C h r o m a T e c h n o l o g y C o r p

62 P r i c e Li s t o f Fi l t e r s Series - Multi-Band Filter Sets with Single Emitters and Single Exciters CFP and DsRed by special request v2 DAPI/FITC/Texas Red /Cy5 This set is spectrally identical to the set but with single-band emitters v2 DAPI/FITC/TRITC This set is spectrally identical to the set but with single-band emitters. This set has been discontinued. Please see replacement set S430/25x Exciter (EX) $ S545/30x Exciter (EX) $ bs Dichroic (BS) $ S480/40m Emitter (EM) $ S620/60m Emitter (EM) $ Total...$ AT350/50x Exciter (EX) $ S403/12x Exciter (EX) $ S501/16x Exciter (EX) $ S568/24x Exciter (EX) $ S654/24x Exciter (EX) $ bs Dichroic (BS) $ S475/20m Emitter (EM) $ S535/30m Emitter (EM) $ S610/40m Emitter (EM) $ S710/60m Emitter (EM) $ Total...$ AT350/50x Exciter (EX) $ S484/15x Exciter (EX) $ S555/25x Exciter (EX) $ bs Dichroic (BS) $ S457/17m Emitter (EM) $ S517/30m Emitter (EM) $ S605/40m Emitter (EM) $ Total... J u n e

63 C h r o m a Te c h n o l o g y Co r p Series - Multi-Band Filter Sets with Single Emitters and Single Exciters 86014v2 DAPI/FITC/Texas Red This set has been discontinued. Please see replacement set FITC/Cy3 /Cy5 This set has been discontinued. Please see replacement set FITC/Texas Red This set has been discontinued. Please see replacement set AT350/50x Exciter (EX) $ S492/18x Exciter (EX) $ S572/23x Exciter (EX) $ bs Dichroic (BS) $ S465/30m Emitter (EM) $ S535/40m Emitter (EM) $ S630/60m Emitter (EM) $ Total... S484/15x Exciter (EX) $ S555/25x Exciter (EX) $ S647/26x Exciter (EX) $ bs Dichroic (BS) $ S517/30m Emitter (EM) $ S605/40m Emitter (EM) $ S700/45m Emitter (EM) $ Total... S492/18x Exciter (EX) $ S572/23x Exciter (EX) $ bs Dichroic (BS) $ S530/35m Emitter (EM) $ S630/60m Emitter (EM) $ Total C h r o m a T e c h n o l o g y C o r p

64 P r i c e Li s t o f Fi l t e r s Series - Multi-Band Filter Sets with Single Emitters and Single Exciters FITC/TRITC v2 This set has been discontinued. Please see replacement set GFP/mRFP1 This set has been discontinued. Please see replacement set S484/15x Exciter (EX) $ S555/28x Exciter (EX) $ bs Dichroic (BS) $ S517/30m Emitter (EM) $ S605/40m Emitter (EM) $ Total... S470/30x Exciter (EX) $ S565/55x Exciter (EX) $ bs Dichroic (BS) $ S510/30m Emitter (EM) $ S650/75m Emitter (EM) $ Total... J u n e

65 C h r o m a Te c h n o l o g y Co r p Series - Quad Filter Set with Single Band Exciters Originally designed for Comparative Genomic Hybridization the filter sets in this series, like those in the 51 and 61 series have become important in a wide variety of applications. The factors common to each of the 80 series filter sets is the use of a multiband beamsplitter and emission filter combination which remain stable in the body of the fluorescence microscope. The use of a stable beamsplitter and emission filter guarantees registration of the various images. The single, and sometimes multi-band excitation filters are mounted in a filter wheel or a filter slider. By changing excitation filters one seeks to excite each fluorochrome in succession, capturing a single image and combining those images with the help of imaging software. There can be some bleeding of one fluorochrome into the image of a shorter wavelength fluorochrome. This is sometimes inevitable, as is the case with Propidium Iodide and FITC v2 DAPI/FITC/Texas Red /Cy5 Quad This set is spectrally identical to the set but with a quad emitter. AT350/50x Exciter (EX) $ S403/12x Exciter (EX) $ S501/16x Exciter (EX) $ S568/24x Exciter (EX) $ S654/24x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ C h r o m a T e c h n o l o g y C o r p

66 P r i c e Li s t o f Fi l t e r s Series - ET Sputtered Multi-Band Sets This series of multi-band filter sets feature our high performance ET sputter coatings. Included are dual, triple and quad-band sets, all of which use single-band excitation and emission filters. These require the use of filter wheels or sliders, or image-splitting devices to hold multiple individual filters. The polychroic beamsplitters remain stationary in a filter cube or similar holder. These sets are image-registered and are particularly useful for co-localization studies, FRET, and simultaneous imaging in live cell applications using image-splitting devices Sedat Quad - ET Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample Dual CFP/YFP - ET Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample. This set replaces discontinued sets: 86002v1, 86002v CFP/YFP/mCherry - ET Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample. This set replaces discontinued set: AT350/50x Exciter (EX) $ ET402/15x Exciter (EX) $ ET490/20x Exciter (EX) $ ET555/25x Exciter (EX) $ ET645/30x Exciter (EX) $ T89100bs Dichroic (BS) $ ET455/50m Emitter (EM) $ ET525/36m Emitter (EM) $ ET605/52m Emitter (EM) $ ET705/72m Emitter (EM) $ Total...$ ET430/24x Exciter (EX) $ ET500/20x Exciter (EX) $ bs Dichroic (BS) $ ET470/24m Emitter (EM) $ ET535/30m Emitter (EM) $ Total...$ ET430/24x Exciter (EX) $ ET500/20x Exciter (EX) $ ET572/35x Exciter (EX) $ bs Dichroic (BS) $ ET470/24m Emitter (EM) $ ET535/30m Emitter (EM) $ ET632/60m Emitter (EM) $ Total...$ J u n e

67 C h r o m a Te c h n o l o g y Co r p Series - ET Sputtered Multi-Band Sets This series of multi-band filter sets feature our high performance ET sputter coatings. Included are dual, triple and quad-band sets, all of which use single-band excitation and emission filters. These require the use of filter wheels or sliders, or image-splitting devices to hold multiple individual filters. The polychroic beamsplitters remain stationary in a filter cube or similar holder. These sets are image-registered and are particularly useful for co-localization studies, FRET, and simultaneous imaging in live cell applications using image-splitting devices CFP/YFP/DsRed Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample DAPI/FITC/TRITC - ET Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample. This set replaces discontinued set: 86013v DAPI/FITC/Texas Red - ET Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample. This set replaces discontinued set: 86014v2 ET430/24x Exciter (EX) $ ET500/20x Exciter (EX) $ ET555/20x Exciter (EX) $ bs Dichroic (BS) $ ET470/24m Emitter (EM) $ ET535/30m Emitter (EM) $ ET600/50m Emitter (EM) $ Total...$ ET402/15x Exciter (EX) $ ET482/28x Exciter (EX) $ ET545/25x Exciter (EX) $ bs Dichroic (BS) $ ET455/50m Emitter (EM) $ ET525/36m Emitter (EM) $ ET605/70m Emitter (EM) $ Total...$ ET402/15x Exciter (EX) $ ET490/20x Exciter (EX) $ ET572/35x Exciter (EX) $ bs Dichroic (BS) $ ET455/50m Emitter (EM) $ ET535/50m Emitter (EM) $ ET632/60m Emitter (EM) $ Total...$ C h r o m a T e c h n o l o g y C o r p

68 P r i c e Li s t o f Fi l t e r s Series - ET Sputtered Multi-Band Sets This series of multi-band filter sets feature our high performance ET sputter coatings. Included are dual, triple and quad-band sets, all of which use single-band excitation and emission filters. These require the use of filter wheels or sliders, or image-splitting devices to hold multiple individual filters. The polychroic beamsplitters remain stationary in a filter cube or similar holder. These sets are image-registered and are particularly useful for co-localization studies, FRET, and simultaneous imaging in live cell applications using image-splitting devices FITC/Cy3/Cy5 - ET Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample. This set replaces discontinued set: FITC/Texas Red - ET Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample. This set replaces discontinued set: FITC/TRITC - ET Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample. This set replaces discontinued sets: 86007, GFP/mCherry - ET Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample. This set replaces discontinued set: ET470/40x Exciter (EX) $ ET545/25x Exciter (EX) $ ET640/30x Exciter (EX) $ bs Dichroic (BS) $ ET520/40m Emitter (EM) $ ET595/50m Emitter (EM) $ ET690/50m Emitter (EM) $ Total...$ ET490/20x Exciter (EX) $ ET572/35x Exciter (EX) $ BS Dichroic (BS) $ ET535/50m Emitter (EM) $ ET632/60m Emitter (EM) $ Total...$ ET470/40x Exciter (EX) $ ET545/25x Exciter (EX) $ bs Dichroic (BS) $ ET520/40m Emitter (EM) $ ET605/70m Emitter (EM) $ Total...$ ET470/40x Exciter (EX) $ ET572/35x Exciter (EX) $ bs Dichroic (BS) $ ET525/50m Emitter (EM) $ ET632/60m Emitter (EM) $ Total...$ J u n e

69 C h r o m a Te c h n o l o g y Co r p Series - ET Sputtered Multi-Band Sets This series of multi-band filter sets feature our high performance ET sputter coatings. Included are dual, triple and quad-band sets, all of which use single-band excitation and emission filters. These require the use of filter wheels or sliders, or image-splitting devices to hold multiple individual filters. The polychroic beamsplitters remain stationary in a filter cube or similar holder. These sets are image-registered and are particularly useful for co-localization studies, FRET, and simultaneous imaging in live cell applications using image-splitting devices DAPI/FITC/TRITC FISH - ET Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample DAPI/FITC/Texas Red FISH - ET Please see the Excitation Intensity Note on page 82 for information on how to avoid rapid photobleaching of sample dbx Exciter (EX) $ tbx Exciter (EX) $ ET376/30x Exciter (EX) $ ET485/20x Exciter (EX) $ ET550/15x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ dbx Exciter (EX) $ tbx Exciter (EX) $ ET376/30x Exciter (EX) $ ET490/20x Exciter (EX) $ ET572/35x Exciter (EX) $ bs Dichroic (BS) $ m Emitter (EM) $ Total...$ C h r o m a T e c h n o l o g y C o r p

70 P r i c e Li s t o f Fi l t e r s Series - Holders (Cubes & Rings) We either manufacture locally or purchase from the microscope manufacturer cubes that are designed for most of the currently used fluorescence microscopes. Chroma Technology Corp produces many standard filter sets and a few custom designs for Leica Microsystems. Both Carl Zeiss and Nikon Corp. offer extensive selections of our filter sets for their new microscope models. Olympus dealers and distributors have access to our products through Olympus America and directly from us. We encourage you to work with your local dealer or representative when purchasing filter sets manufactured by us mm Nikon Microscopes (Diaphot, TMD) **Recommend installation at Chroma to ensure proper gluing and alignment. Total...$ Nikon Eclipse/Quadfluor This metal cube is also compatible with Nikon s E400, E600, E800 and E1000 upright microscopes, as well as the TE200 and TE300 inverted Eclipse microscopes. Please note that this Eclipse/Quadfluor cube is NOT intended for use in Nikon s Eclipse TE2000 or TI2000 microscopes and should not be confused with the cube. Users should also note that early versions of Diaphot microscopes used our (18mm) cubes, but many have been converted in order to be consistent with all later Diaphot models, which use this cube. Please contact your Nikon dealer to purchase this cube empty, or mounted with the following Chroma filter sets: 11000, 11001, 11003, 11004, 11007, 31000, 31001, 31002a, 31004, 31021, 31044, 41001, 40112c, 41004, 41007a, 41008, 41017, 41018, 41025, 41028, 51000, 51004v2, 51006, 61000v2, **Recommend installation at Chroma to ensure proper gluing and alignment. Total...$ Olympus BH2 **Recommend installation at Chroma to ensure proper gluing and alignment. Total...$ Olympus IMT2 Filter Cube **Recommend installation at Chroma to ensure proper gluing and alignment. Total...$ J u n e

71 C h r o m a Te c h n o l o g y Co r p Series - Holders (Cubes & Rings) We either manufacture locally or purchase from the microscope manufacturer cubes that are designed for most of the currently used fluorescence microscopes. Chroma Technology Corp produces many standard filter sets and a few custom designs for Leica Microsystems. Both Carl Zeiss and Nikon Corp. offer extensive selections of our filter sets for their new microscope models. Olympus dealers and distributors have access to our products through Olympus America and directly from us. We encourage you to work with your local dealer or representative when purchasing filter sets manufactured by us Leica Ploempak **Recommend installation at Chroma to ensure proper gluing and alignment. However, Chroma can only align Ploem cubes with 3 screws. Those with 4 screws must be sent to Leica. Total...$ Leica Aristoplan Filter Cube **Recommend installation at Chroma to ensure proper gluing and alignment. Total...$ Zeiss Axioline 3FL Slider Total...$ Leica DM Filter Cube **Recommend installation at Chroma to ensure proper gluing and alignment. Total...$ Leica DMIRB Filter Cube **Recommend installation at Chroma to ensure proper gluing and alignment. Total...$ Zeiss Axioline 4FL Slider Total...$ C h r o m a T e c h n o l o g y C o r p

72 P r i c e Li s t o f Fi l t e r s Series - Holders (Cubes & Rings) We either manufacture locally or purchase from the microscope manufacturer cubes that are designed for most of the currently used fluorescence microscopes. Chroma Technology Corp produces many standard filter sets and a few custom designs for Leica Microsystems. Both Carl Zeiss and Nikon Corp. offer extensive selections of our filter sets for their new microscope models. Olympus dealers and distributors have access to our products through Olympus America and directly from us. We encourage you to work with your local dealer or representative when purchasing filter sets manufactured by us Zeiss Axioline 5FL Slider Total...$ Zeiss Axioline 6FL Slider Total...$ Zeiss Standard 2FL Slider Total...$ Zeiss Axio Screws into turret Total...$ for Zeiss Axio 2 DISCONTINUED - See Total...$ Olympus CK40 **Recommend installation at Chroma to ensure proper gluing. Total...$ J u n e

73 C h r o m a Te c h n o l o g y Co r p Series - Holders (Cubes & Rings) We either manufacture locally or purchase from the microscope manufacturer cubes that are designed for most of the currently used fluorescence microscopes. Chroma Technology Corp produces many standard filter sets and a few custom designs for Leica Microsystems. Both Carl Zeiss and Nikon Corp. offer extensive selections of our filter sets for their new microscope models. Olympus dealers and distributors have access to our products through Olympus America and directly from us. We encourage you to work with your local dealer or representative when purchasing filter sets manufactured by us BX2 (U-MF2) for Olympus BX and IX models Total...$ Nikon SMZ Stereo **Recommend installation at Chroma to ensure proper gluing. Total...$ Nikon TE2000/TI2000 This plastic cube is also made for use in several of the 50i, 80i and 90i upright microscopes. Please note that this cube is intended for the Eclipse TE/TI2000 microscopes and should NOT be confused with Chroma s Eclipse/ Quafluor cube. Please contact your Nikon dealer to purchase this cube empty, or mounted with the following Chroma filter sets: 11000, 11001, 11002, 11004, 11007, 31000, 31001, 31002a, 31004, 31021, 31044v2, 41001, 41002c, 41004, 41007a, 41008, 41017, 41018, 41028, 49000, 49001, 49002, 49003, 49005, 49006, 49007, 49008, 51000, 51004v2, 51006, 61000v2, Total...$ Olympus SZX RFL3 emission holder Total...$ Olympus SZX RFL3 excitation holder Total...$ C h r o m a T e c h n o l o g y C o r p

74 P r i c e Li s t o f Fi l t e r s Series - Holders (Cubes & Rings) We either manufacture locally or purchase from the microscope manufacturer cubes that are designed for most of the currently used fluorescence microscopes. Chroma Technology Corp produces many standard filter sets and a few custom designs for Leica Microsystems. Both Carl Zeiss and Nikon Corp. offer extensive selections of our filter sets for their new microscope models. Olympus dealers and distributors have access to our products through Olympus America and directly from us. We encourage you to work with your local dealer or representative when purchasing filter sets manufactured by us Leica DM 2500, 3000, 4000, 5000, 6000 **Recommend installation at Chroma to ensure proper gluing and alignment. Total...$ Nikon prism-to-dichroic conversion mount This is not a holder for standard fluorescence filters. This is a custom modification to take the place of the bottom prism position in the TE2000. Note that the manufacturers warn that removing the original prism may void warranty. Total...$ Zeiss Axio inverted 2F Total...$ Zeiss Axio Imager with tilted emission filter Total...$ Olympus XL This cube requires larger filters - Total...$ J u n e

75 C h r o m a Te c h n o l o g y Co r p Series - Holders (Cubes & Rings) We either manufacture locally or purchase from the microscope manufacturer cubes that are designed for most of the currently used fluorescence microscopes. Chroma Technology Corp produces many standard filter sets and a few custom designs for Leica Microsystems. Both Carl Zeiss and Nikon Corp. offer extensive selections of our filter sets for their new microscope models. Olympus dealers and distributors have access to our products through Olympus America and directly from us. We encourage you to work with your local dealer or representative when purchasing filter sets manufactured by us Laser TIRF for Nikon TE/Ti2000 This cube is intended for laser TIRF and will have the set mirror pre-installed and laser aligned prior to shipping. Standard (non-laser) optics can be used in this cube ONLY for non laser applications. Please contact us for a quote for the appropriate optics for your application. Patent pending. Total...$ Nikon Excitation Filter Ring 18mm (for cube) Total...$ Nikon Emission Filter Ring 18mm (for cube) Total...$ Nikon Excitation Filter Ring 25mm (for cube) Total...$ Nikon Emission Filter Ring 25mm (for cube) Total...$ C h r o m a T e c h n o l o g y C o r p

76 P r i c e Li s t o f Fi l t e r s Series - Holders (Cubes & Rings) We either manufacture locally or purchase from the microscope manufacturer cubes that are designed for most of the currently used fluorescence microscopes. Chroma Technology Corp produces many standard filter sets and a few custom designs for Leica Microsystems. Both Carl Zeiss and Nikon Corp. offer extensive selections of our filter sets for their new microscope models. Olympus dealers and distributors have access to our products through Olympus America and directly from us. We encourage you to work with your local dealer or representative when purchasing filter sets manufactured by us Zeiss Axio Filter Retaining Ring 25mm (for all cubes and sliders) Total...$ BX3 for Olympus BX3 fluorescence illuminators This cube is designed to be inserted or removed from the filter turret without the need for tools. Currently supported illuminators include BX3-RFAS, BX3-URA and BX3-RFAA Total...$ J u n e

77 C h r o m a Te c h n o l o g y Co r p Series - Diagnostic Tools Fluoro4 Control Slide Set Total...$ Autofluorescent Plastic Slides These are intended to determine the consistency and evenness of illumination of excitation light on a fluorescent sample. However, we cannot make any guarantees about the substances these slides are manufactured with, nor what their emission properties are. Because we do not alter or dictate the materials used in their construction, we are unable to support them in any fashion beyond making them available. They can also be obtained at the many trade shows and conferences we attend. Total...$ C h r o m a T e c h n o l o g y C o r p

78 P r i c e Li s t o f Fi l t e r s G-Series - Modified goggles for macro viewing Modified welder s goggles with longpass emission glass built in to allow macro viewing of whole animals. G-515GFP Goggles For use with a blue excitation source. Goggles are OG515, 3mm thick, image quality, 50mm diameter. Total...$ G-570RFP Goggles For use with a blue or green excitation source. Goggles are OG570, 3mm thick, image quality, 50mm diameter. Total...$ G-590RFP Goggles For use with a blue, green or yellow excitation source. Goggles are OG590, 3mm thick, image quality, 50mm diameter. Total...$ J u n e

79 C h r o m a Te c h n o l o g y Co r p MF100 - Filter Sets for Multi-Color FISH (Spec d by Perceptive Scientific for Vysis fluorochromes) Specified by Perceptive Scientific for use with Vysis fluorochromes. Please contact Vysis for the official Vysis M-FISH filter sets. MF101 Spectrum Green HQ493/16x Exciter (EX) $ Q507LP Dichroic (BS) $ HQ527/30m Emitter (EM) $ Total...$ MF102 Spectrum Orange HQ548/20x Exciter (EX) $ Q565LP-AR Dichroic (BS) $ HQ580/14m Emitter (EM) $ Total...$ C h r o m a T e c h n o l o g y C o r p

80 P r i c e Li s t o f Fi l t e r s SP100 - Filter Sets for Multi-Color FISH Designed for Dr. Michael Speicher, University of Munich. Each of the components of these filter sets (except for the SP100 set) are even steeper than the 41 series. This allows for color discrimination when using up to seven different fluorochromes. To increase brightness, each of the components is anti-reflected. Again, the SP100 is the exception. SP100v2 DAPI AT350/50x Exciter (EX) $ dclp-AR Dichroic (BS) $ D470/40m-AR Emitter (EM) $ Total...$ SP101 FITC HQ471/39x Exciter (EX) $ Q497LP Dichroic (BS) $ HQ522/40m Emitter (EM) $ Total...$ SP102v1 Cy3 v1 HQ546/11x Exciter (EX) $ Q557LP Dichroic (BS) $ HQ567/15m Emitter (EM) $ Total...$ SP102v2 Cy3 v2 HQ530/30x Exciter (EX) $ Q553LP Dichroic (BS) $ HQ565/18m Emitter (EM) $ Total...$ SP103v1 Cy3.5 v1 HQ581/10x Exciter (EX) $ Q593LP Dichroic (BS) $ HQ617/40m Emitter (EM) $ Total...$ SP103v2 Cy3.5 v2 HQ580/13x Exciter (EX) $ Q593LP Dichroic (BS) $ HQ617/40m Emitter (EM) $ Total...$ J u n e

81 C h r o m a Te c h n o l o g y Co r p SP100 - Filter Sets for Multi-Color FISH Designed for Dr. Michael Speicher, University of Munich. Each of the components of these filter sets (except for the SP100 set) are even steeper than the 41 series. This allows for color discrimination when using up to seven different fluorochromes. To increase brightness, each of the components is anti-reflected. Again, the SP100 is the exception. SP104v1 Cy5 v1 HQ622/36x Exciter (EX) $ Q647LP Dichroic (BS) $ HQ667/30m Emitter (EM) $ Total...$ SP104v2 Cy5 v2 HQ630/20x Exciter (EX) $ Q649LP Dichroic (BS) $ HQ667/30m Emitter (EM) $ Total...$ SP105 Cy5.5 HQ682/12x Exciter (EX) $ Q697LP Dichroic (BS) $ HQ721/42m Emitter (EM) $ Total...$ SP106 Cy7 HQ740/35x Exciter (EX) $ Q765LP Dichroic (BS) $ HQ795/50m Emitter (EM) $ Total...$ SP107 Texas Red HQ602/13x Exciter (EX) $ Q615LP Dichroic (BS) $ HQ631/23m Emitter (EM) $ Total...$ SP108 Laser-Pro HQ782/20x Exciter (EX) $ Q800LP Dichroic (BS) $ HQ817/25m Emitter (EM) $ Total...$ C h r o m a T e c h n o l o g y C o r p

82 P r i c e Li s t o f Fi l t e r s LASER APPLICATIONS Standard epifluorescence exciters and mirrors do not work with laser applications. Laser optics require special consideration, particularly for clean up filters and mirrors. To request that we put together a combination for your specific application, please laser@chroma.com, confocal@chroma.com or call with the following information: type of microscope (confocal, epifluorescence, tirf ) brand of microscope laser type (plasma, diode, HeNe, Krypton/Argon mix, Ti:Sapphire ) fluorochrome to be used absorption Glass Please specify thickness (e.g. 1 mm, 2 mm, 3 mm) when ordering. Absorption characteristics are thickness-dependent. Thinner pieces transmit higher and block lower, while thicker pieces transmit lower and block higher. UV PROTECTION Longpass glass PART NUMBER IMAGE QUALITY (25 mm or less) IMAGE QUALITY (26 mm - 50 mm) NON-IMAGE QUALITY (25 mm or less) NON-IMAGE QUALITY (26 mm - 50 mm) GG GG GG GG GG GG OG OG OG OG OG RG RG RG RG RG RG RG RG IR PROTECTION Shortpass Glass PART NUMBER IMAGE QUALITY (25 mm or less) IMAGE QUALITY (26 mm - 50 mm) NON-IMAGE QUALITY (25 mm or less) NON-IMAGE QUALITY (26 mm - 50 mm) KG KG KG UV TRANSMISSION Shortpass Glass PART NUMBER (25 mm or less) (26 mm - 50 mm) UG J u n e

83 C h r o m a Te c h n o l o g y Co r p Excitation Intensity Note: How to Avoid Rapid Photobleaching of Sample. High levels of excitation light intensity (such as that transmitted by our ET-series excitation filters) could, under some circumstances, lead to rapid photobleaching or phototoxic damage to the sample, and/or high levels of autofluorescent signal. The use of neutral density (ND) filters in the excitation light path may be required to minimize these effects. Furthermore, the use of a range of multiple ND filters can allow for application- or sample-specific customization of excitation light intensities to maximize signal-to-noise. See the Series. Page C h r o m a T e c h n o l o g y C o r p

84 P r i c e Li s t o f Fi l t e r s Discontinued and Special Order Sets TYpe Discontinued and Special Order Sets Recommended Set See Page Discontinued Allophycocyanin (APC) See replacement set Page 25 Discontinued Quinacrine Mustard See replacement set Page 14 Discontinued Hydroxycoumarin See replacement set Page 34 Discontinued Chlorophyll Bandpass Emission See replacement set Page 30 Discontinued 31017a Chlorophyll Longpass Emission See replacement set Page 30 Discontinued NBD / HPTS Contact us for a recommended replacement. Discontinued GFP Contact us for a recommended replacement. Discontinued Blue GFP Bandpass Emission See replacement set Page 34 Discontinued DiA See replacement set Page 15 Discontinued Narrow-band GFP for eliminating auto-fluorescence See replacement set Page 27 Special Order CTC Available by special request or contact us for a recommended replacement. Special Order 31036v2 DEAC Available by special request or see replacement set. Discontinued Red See replacement set Page 29 Discontinued 31047v2 Coumarin See replacement set Page 34 Discontinued Cyan/Topaz Energy Transfer See replacement set Page 35 Discontinued DHE Contact us for a recommended replacement. Special Order 31062v2 Fast Red Available by special request or contact us for a recommended replacement. Special Order GFP Bandpass Emission Available by special request or contact us for a recommended replacement. Discontinued GFP Longpass Emission Contact us for a recommended replacement. Discontinued Qdot 850 filter set with 20 nm wide emission Contact us for a recommended replacement. Discontinued Qdot 800 Contact us for a recommended replacement. Discontinued Adirondack Green Contact us for a recommended replacement. Discontinued Hops Yellow Contact us for a recommended replacement. Discontinued Birch Yellow Contact us for a recommended replacement. Discontinued Fort Orange Contact us for a recommended replacement. Discontinued Rome Red Contact us for a recommended replacement. Discontinued Empire Red Contact us for a recommended replacement. Discontinued Macoun Red Contact us for a recommended replacement. Discontinued Jonamac Red Contact us for a recommended replacement. Discontinued TRITC (Rhodamine)/DiI/Cy3 See replacement set Page 24 Discontinued 41002a TRITC (Rhodamine)/DiI with red-shifted emission filter See replacement set Page 32 Discontinued R and B Phycoerythrin Hg light source See replacement set Page 33 Discontinued Ethidium Bromide See replacement set Page 24 Discontinued Cy7 See replacement set Page 33 Discontinued Resorufin See replacement set Page 29 Special Order wtgfp Longpass Emission Available by special request or contact us for a recommended replacement. Special Order Visual Cy5 (GLF) Available by special request or see replacement set Page 25 Special Order Cy5.5 (Red Shifted) Available by special request or see replacement set Page 27 Special Order Calcium Crimson Available by special request or see replacement set Page 29 Special Order Yellow GFP LP (10/C Topaz) Available by special request or see replacement set Page 34 Discontinued Indo-Cyanine Green See replacement set Page 34 Discontinued Narrow Excitation Cy5 See replacement set Page 33 Special Order Narrow Excitation (Hg) DsRed Available by special request or contact us for a recommended replacement. Discontinued Li-Cor for IR Dye 800 See replacement set Page 34 Discontinued Spectrum Gold See replacement set Page 33 J u n e

85 C h r o m a Te c h n o l o g y Co r p Discontinued and Special Order Sets TYpe Discontinued and Special Order Sets Recommended Set See Page Special Order AmCyan1 Available by special request or contact us for a recommended replacement. Special Order ZsGreen1 Available by special request or contact us for a recommended replacement. Special Order ZsYellow1 Available by special request or contact us for a recommended replacement. Special Order AsRed2 Available by special request or contact us for a recommended replacement. Discontinued BioPixels w/longpass Emission Filter Contact us for a recommended replacement. Discontinued BioPixels 485 Contact us for a recommended replacement. Discontinued BioPixels 520 Contact us for a recommended replacement. Discontinued BioPixels 570 Contact us for a recommended replacement. Discontinued BioPixels 595 Contact us for a recommended replacement. Discontinued BioPixels 620 Contact us for a recommended replacement. Discontinued DAPI/Propidium Iodide See replacement set Page 36 Special Order 51004v2 FITC/TRITC Available by special request or see replacement set Page 42 Discontinued FITC/Propidium Iodide See replacement set Page 37 Special Order Cy3 /Cy5 Available by special request or contact us for a recommended replacement. Special Order FITC/Cy5 Available by special request or contact us for a recommended replacement. Discontinued DAPI/Chlorophyll Contact us for a recommended replacement. Special Order DAPI/Cy3 Available by special request or contact us for a recommended replacement. Special Order DAPI/GFP Available by special request or contact us for a recommended replacement. Special Order DAPI/Cy5 Available by special request or contact us for a recommended replacement. Discontinued BFP/GFP Contact us for a recommended replacement. Special Order FITC/DiI Available by special request or contact us for a recommended replacement. Special Order Lucifer Yellow/Rhodamine or DiI Available by special request or contact us for a recommended replacement. Special Order CFP/YFP Available by special request or see replacement set Page 42 Special Order EGFP/DsRed Available by special request or see replacement set Page 42 Special Order 52004v2 FITC/TRITC W/Single Band Exciters Available by special request or see replacement set Page 42 Special Order FITC/PI W/Single Band Exciters Available by special request or see replacement set Page 43 Special Order FITC/Texas Red W/Single Band Exciters Available by special request or see replacement set Page 43 Special Order CFP/YFP with Single-Band Exciters Available by special request or see replacement set Page 42 Special Order CFP/DsRed with Single-Band Exciters Available by special request or contact us for a recommended replacement. Special Order EGFP/DsRed with Single-Band Exciters Available by special request or see replacement set. Special Order 61000v2 DAPI/FITC/TRITC or Cy3 Available by special request or see replacement set Page C h r o m a T e c h n o l o g y C o r p

86 P r i c e Li s t o f Fi l t e r s Discontinued and Special Order Sets TYpe Discontinued and Special Order Sets Recommended Set See Page Special Order DAPI/FITC/Propidium Iodide Available by special request or see replacement set Page 47 Special Order DAPI/FITC/Texas Red Available by special request or see replacement set Page 47 Special Order DAPI/FITC/Cy5 Available by special request or contact us for a recommended replacement. Special Order DAPI/Cy3 /Cy5 Available by special request or contact us for a recommended replacement. Special Order FITC/Cy3 /Cy5 Available by special request or contact us for a recommended replacement. Special Order FITC/Texas Red /Cy5 Available by special request or contact us for a recommended replacement. Special Order CFP/YFP/DsRed Available by special request or see replacement set Page 47 Special Order CFP/YFP/Cy5 Available by special request or contact us for a recommended replacement. Special Order DAPI/FITC/Cy3 Available by special request or see replacement set. Special Order 62000v3 DAPI/FITC/TRITC or Cy3 with single band exciters Available by special request or see replacement set Page 47 Special Order DAPI/FITC/Propidium Iodide Available by special request or see replacement set Page 47 Special Order 62002v2 DAPI/FITC/Texas Red w/single-band exciters Available by special request or see replacement set Page 47 Discontinued Aqua/Gold/Far Red w/single band exciters Contact us for a recommended replacement. Discontinued 71000av2 Fura 2 with wide band emission filter. See set# See replacement set Page 54 Discontinued 71000v2 Fura 2. See set# See replacement set Page 54 Discontinued BCECF Contact us for a recommended replacement. Discontinued 71001a BCECF with wider D440 filter for more balanced excitation See replacement set Page 52 Discontinued INDO Contact us for a recommended replacement. Discontinued 71002a INDO - No emission beamsplitter See replacement set Page 52 Special Order Fura Red Available by special request or contact us for a recommended replacement. Discontinued Cameleons 2 Contact us for a recommended replacement. Discontinued 71007a Cameleons 2 - No Emission Beamsplitter See replacement set Page 52 Discontinued Beta Lactamase Ratiometric Contact us for a recommended replacement. Discontinued 71008a Beta Lactamase Ratiometric See replacement set Page 52 Discontinued LS/P Cyan/Topaz Dual Excitation Contact us for a recommended replacement. Discontinued JC1 Contact us for a recommended replacement. Discontinued 71010a JC1 See replacement set Page 53 Discontinued Sapphire/Topaz Dual Excitation Contact us for a recommended replacement. Discontinued Qdot Multiple Emission Set Contact us for a recommended replacement. Discontinued 72000v2 FURA 2 and BCECF Contact us for a recommended replacement. Discontinued 73000v2 FURA 2 and Rhodamine Contact us for a recommended replacement. Discontinued 74000v2 FURA 2 and Fluo 3 Contact us for a recommended replacement. Special Order Supplementary Emission Filters for Filter Set Available by special request or contact us for a recommended replacement. J u n e

87 C h r o m a Te c h n o l o g y Co r p Discontinued and Special Order Sets TYpe Discontinued and Special Order Sets Recommended Set See Page Special Order Supplementary Emission Filters for Filter Set Available by special request or contact us for a recommended replacement. Special Order JP3 - EGFP and YFP Available by special request or contact us for a recommended replacement. Special Order 86002v1 JP4 - CFP and YFP Available by special request or see replacement set Page 65 Special Order 86002v2 JP4 - CFP and YFP Available by special request or see replacement set Page 65 Special Order BFP and EGFP Available by special request or contact us for a recommended replacement. Special Order 86004v1 JP5 - UVGFP (Sapphire)/CFP/YFP Available by special request or contact us for a recommended replacement. Special Order 86004v2 JP5 - UVGFP (Sapphire)/CFP/YFP Available by special request or contact us for a recommended replacement. Special Order BFP and YFP Available by special request or contact us for a recommended replacement. Special Order CFP/YFP/DsRed Available by special request or see replacement set Page 65 Special Order EGFP/DsRed Available by special request or see replacement set Page 67 Special Order 86008v1 CFP/YFP and Cy5 v1 Available by special request or contact us for a recommended replacement. Special Order BFP/GFP and DsRed Available by special request or contact us for a recommended replacement. Discontinued BFP (or DAPI)/CFP/YFP and DsRed (or Texas Red ) Contact us for a recommended replacement. Special Order CFP and DsRed Available by special request or contact us for a recommended replacement. Discontinued 86013v2 DAPI/FITC/TRITC See replacement set Page 66 Discontinued 86014v2 DAPI/FITC/Texas Red See replacement set Page 66 Discontinued UV/Sapp.GFP/DsRed Contact us for a recommended replacement. Discontinued FITC/Cy3 /Cy5 See replacement set Page 67 Discontinued FITC/Texas Red See replacement set Page 67 Discontinued FITC/Cy3 Contact us for a recommended replacement. Discontinued FITC/TRITC v2 See replacement set Page 67 Discontinued FITC/Texas Red /Cy5 Contact us for a recommended replacement. Discontinued GFP/mRFP1 See replacement set Page 67 Discontinued Cy3/Cy5 Contact us for a recommended replacement. Discontinued FITC/Cy5 Contact us for a recommended replacement. Discontinued ISO/EGFP Contact us for a recommended replacement. Discontinued YFP/DsRed Contact us for a recommended replacement. Discontinued YFP/Texas Red Contact us for a recommended replacement. 86 C h r o m a T e c h n o l o g y C o r p

88 Fluorochrome List

89 C h r o m a Te c h n o l o g y Co r p Fluorochrome List Fluorochrome Excitation Emission recommended Set Alternative sets 5-(and 6)-carboxy-2,7 -dichlorofluorescein Acridine Orange + DNA Alexa Fluor v2 Alexa Fluor , Alexa Fluor Alexa Fluor Alexa Fluor b Alexa Fluor b Alexa Fluor Alexa Fluor Alexa Fluor Alexa Fluor Alexa Fluor Alexa Fluor Alexa Fluor Allophycocyanin (APC) AmCyan AsRed Azami Green BODIPY FL/pH Calcein Calcium Green Calcofluor White v v2 Carboxyrhodamine 6G Cerulean CFP Cy Cy Cy Cy Cy Cy DAPI Di-8-ANEPPS non-ratiometric DiA DiD DiI DiO DiR Draq DsRed DyLight v2 DyLight DyLight DyLight b DyLight DyLight DyLight C h r o m a T e c h n o l o g y C o r p

90 P r i c e Li s t o f Fi l t e r s Fluorochrome List Fluorochrome Excitation Emission recommended Set Alternative sets DyLight DyLight DyLight EBFP EGFP Emerald GFP Eosin Ethidium Bromide Ethidium homidimer-1/dna EYFP/pH FAM FITC FlAsH-CCPFCC Fluo Fluo Fura Red/Ca2+ - saturated Fura Red/Ca2+ -free Fura-2/Ca2+ - free Fura-2/Ca2+ - saturated GFP HEX,SE Hoechst Indocyanine Green JC-1 non-ratiometric JC-1 ratiometric JOE Lucifer Yellow LysoTracker Blue/MeOH LysoTracker Green/pH LysoTracker Red/pH LysoTracker Yellow HCK mcherry mcitrine MitoTracker Deep Red 633/MeOH MitoTracker Green FM/MeOH MitoTracker Orange/MeOH mkate (TagFP635) mko morange mrfp mwasabi NBD X/MeOH Nile Blue Oregon Green Oregon Green Pacific Blue Propidium Iodide Qdot J u n e

91 C h r o m a Te c h n o l o g y Co r p. Fluorochrome List Fluorochrome Excitation Emission recommended Set Alternative sets Qdot Qdot Qdot Qdot Qdot 625 streptavidin/ph Qdot Qdot Qdot R-phycoerythrin ReAsH-CCPGCC Resorufin Rhod Rhodamine Rhodamine 6G Rhodamine Red -X ROX SBFI/Na+ -free SBFI/Na+ -saturated Sulforhodamine SYBR Green I SYTO 9/DNA SYTO TagBFP TagRFP b TAMRA tdtomato TET Tetramethylrhodamine goat anti mouse IgG antibody/ Texas Red Texas Red -X TO-PRO wtgfp X-rhod-1/Ca ZsGreen ZsYellow C h r o m a T e c h n o l o g y C o r p

92 NOTES J u n e

93 Notes 92 C h r o m a T e c h n o l o g y C o r p

94 Chroma Technology Corp (800-8CHROMA) toll free Tel Fax Chroma Technology GmbH Tel Fax Chroma Technology Corp and the Chroma logo are registered trademarks of Chroma Technology Corp