Advanced Technologies Committee

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1 Advanced Technologies Committee Chair: Bert van Duijn Committee report

2 Committee members ( ) ATC comments ECOM Approval 1 Chair Bert van Duijn Netherlands Yes 2 Vice-Chair Brigitte Hamman Switzerland Yes 3 Member Birte Boelt Denmark Yes 4 Member Kent Bradford United States Yes 5 Member Rukui Huang China Yes 6 Member Bob Legro Netherlands Yes 7 Member Harry Nijenstein Netherlands Yes 8 Member Graeme Smith Australia Yes 9 Member Joost van der Burg Netherlands Yes 10 Member Zhujun Zhu China Yes 11 Member Ghasem Tohidloo Iran Yes 12 Member Miecyslaw Grzesik Poland new member Yes 13 Member Karima Bouderhi-Giresse France new member Yes Date - Presenter Name

3 13 ATC Members Switzerland Netherlands USA Iran France Countries Denmark China Australia Poland Date - Presenter Name

4 AIMS of ATC Be a bridge between the Research and Development World and the other research within TCOMs of ISTA. Collect and interpret data and opinions on advanced technologies. Provide information on advanced technologies to the ISTA community Chair or participate in research/tests of advanced technologies in cooperation with other TCOMs and/or act as reviewers. Formulate views on ongoing or future technological developments from a seed testing perspective to aid the ISTA organization. In addition.. ATC will NOT aim at developing ISTA approved or ISTA advised technologies. ATC will NOT aim at producing proposals for the ISTA rules. Method validation: requests are forwarded to other Tcom and coordinator Date - Presenter Name

5 ATC Working groups Imaging technology (seed) image technology overview Web-based information platform + STI summary (due October 2013Bert van Duijn) X-ray seed imaging (scientific) review (due 2013Henrie Bruggink) Multispectral seed imaging STI overview (due October 2013 Birte Boelt) Date - Presenter Name

6 ATC Working groups Chlorofyl fluorescence CF & Seed ripeness (report ready) CF changes during germination (report ready) Concluding summary (due October 2013, Harry Nijenstein) (Single) Seed respiration Awaiting scientific publications (Kent Bradford) Date - Presenter Name

7 Multispectral imaging - illumination with specific wavelengths ultraviolet (UV) near-infrared (NIR) nm

8 Multispectral imaging - information in wavelength bands

9 Multispectral imaging What to be measured? Surface chemistry Topographical texture Spectral texture Colour (or Gray level) Gloss Shape Size Position and orientation

10 Number of pixels Identification of fungi Seed health 450n m 870n m Stemphylium Alternaria Fusarium Cladosporium Verticillium Seed Science and Technology Olesen et al., 2011

11 Identifying weed seeds Purity Festuca rubra, L. L: 6.2; W: 1.0; H: 0.8 Vulpia bromoides L: 6.3; W: 0.7; H: 0.6

12 Caryopsis length Purity and germination? Features: Length of seed (lemma) and caryopsis. Correlates to germination?

13 (Seed) Imaging technology overview Different physical principles for image formation Visible light (including UV and far-red) Reflection images Phase contrast images Fluorescence images (fluorescence lifetime) Raman images (chemical composition) Absorption images Two or more photon fluorescence Infra-red Radiation images (temperature) Water content images Chemical composition Bio-luminescence Radiation images (metabolic activity)

14 X-ray Transmission images(density) Specific wavelengths (absorption/fluorescence?); compound images Metabolic processes through radioactive labelling Electron Transmission images Scanning images NMR Specific compounds imaging Laser ablation imaging Chemical composition images Sound Acoustic images; transmission/reflection Atomic force Mechanical properties image Surface structure image Scanning probe Surface structure image, through optical, electrochemical processes

15 Workshops/ seminars 2012 Seminar in Venlo: New developments and technologies in seed testing 2014 workshop: Image analysis as a tool in seed xxxxx In cooperation with other Tcom(s) (e.g. Health, Moisture, Germination, Vigour, Purity etc; under discussion)

16 Join ATC workgroups! Send your questions on (new) technologies to ATC Explore possibilities for cooperation/ joint projects between ATC and other Tcoms Send in your seed images (see imaging technology list) Become member of an ATC working group Visit ATC general meeting (discussion/information/brainstorm) Today room Lal A1

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