Systematic Workflow via Intuitive GUI. Easy operation accomplishes your goals faster than ever.

Similar documents
3D Measuring Laser Microscope OLS4100. Bringing Answers to the Surface. Precise measurement. Faster operation. High-quality imaging.

3D Measuring Laser Microscope OLS4100. Bringing Answers to the Surface. More precise measurement. Faster operation. High-quality imaging.

Bringing Answers to the Surface

Olympus LEXT OLS 4000 Confocal Laser Microscope

Camera Overview. Digital Microscope Cameras for Material Science: Clear Images, Precise Analysis. Digital Cameras for Microscopy

Camera Overview. Digital Microscope Cameras for Material Science: Clear Images, Precise Analysis. Digital Cameras for Microscopy

Operating Instructions for Zeiss LSM 510

Camera Overview. Digital Microscope Cameras for Material Science: Clear Images, Precise Analysis. Digital Cameras for Microscopy

Huvitz Digital Microscope HDS-5800

Practical work no. 3: Confocal Live Cell Microscopy

Instructions for the Experiment

Variable microinspection system. system125

SUPRA Optix 3D Optical Profiler

Microscopy from Carl Zeiss

ASM Webinar Digital Microscopy for Materials Science

nanovea.com PROFILOMETERS 3D Non Contact Metrology

Super High Vertical Resolution Non-Contact 3D Surface Profiler BW-S500/BW-D500 Series

Fastest high definition Raman imaging. Fastest Laser Raman Microscope RAMAN

Difrotec Product & Services. Ultra high accuracy interferometry & custom optical solutions

Fast Laser Raman Microscope RAMAN

Zeiss LSM 510 Confocor III Training Notes. Center for Cell Analysis & Modeling

Cell Biology and Bioimaging Core

Turnkey Solution for Technical Cleanliness Inspection

LEICA TCS SP5 AOBS TANDEM USER MANUAL

KEYENCE VKX LASER-SCANNING CONFOCAL MICROSCOPE Standard Operating Procedures (updated Oct 2017)

SMX-1000 Plus SMX-1000L Plus

1 Set up the confocal light path for imaging a green dye (Alexa488-EGFP). For example, the

Quick Guide. LSM 5 MP, LSM 510 and LSM 510 META. Laser Scanning Microscopes. We make it visible. M i c r o s c o p y f r o m C a r l Z e i s s

LSM 510 Meta Training Notes

Parallel Mode Confocal System for Wafer Bump Inspection

Confocal imaging on the Leica TCS SP8. 1) Turn the system on. 2) Use TCS user account. 3) Start LAS X software:

Fast Laser Raman Microscope RAMAN

Confocal NEXIV VMZ-K Series. CNC Video Measuring System CONFOCAL NEXIV. VMZ-K Series

Horiba Jobin-Yvon LabRam Raman Confocal Microscope (GERB 120)

PICO MASTER 200. UV direct laser writer for maskless lithography

Leica TCS SP8 Quick Start Guide

MIF ZEISS LSM510 CONFOCAL USER PROTOCOL

Confocal NEXIV VMZ-K Series. CNC Video Measuring System CONFOCAL NEXIV. VMZ-K Series

Leica TCS SP8 Quick Start Guide

Proudly serving laboratories worldwide since 1979 SPECIFICATIONS

LSM 780 Confocal Microscope Standard Operation Protocol

Bi/BE 227 Winter Assignment #3. Adding the third dimension: 3D Confocal Imaging

Development of a new multi-wavelength confocal surface profilometer for in-situ automatic optical inspection (AOI)

Nanonics Systems are the Only SPMs that Allow for On-line Integration with Standard MicroRaman Geometries

OLYMPUS Digital Cameras for Materials Science Applications: Get the Best out of Your Microscope

Be aware that there is no universal notation for the various quantities.

b. Turn the power switch and key to on position for blue laser.

LSM 510 Training Notes

Characterization Microscope Nikon LV150

Leica SP8 Resonant Confocal. Quick-Start Guide

Supplemental Figure 1: Histogram of 63x Objective Lens z axis Calculated Resolutions. Results from the MetroloJ z axis fits for 5 beads from each

User manual for Olympus SD-OSR spinning disk confocal microscope

Measurement of Microscopic Three-dimensional Profiles with High Accuracy and Simple Operation

DIC Imaging using Laser Scanning Microscopes (LSMs) on Axio Imager Stands

Training Guide for Carl Zeiss LSM 5 LIVE Confocal Microscope

Opti 415/515. Introduction to Optical Systems. Copyright 2009, William P. Kuhn

Leica SP8 TCS Users Manual

Optical Components for Laser Applications. Günter Toesko - Laserseminar BLZ im Dezember

VOLLSTÄDT DIAMANT GmbH. DiaInspect.OSM Automated particle analysis for superabrasives and surface analysis. Operation guide Version 1.2.

The Zeiss AiryScan System, Confocal Four.

Horiba LabRAM ARAMIS Raman Spectrometer Revision /28/2016 Page 1 of 11. Horiba Jobin-Yvon LabRAM Aramis - Raman Spectrometer

CRISATEL High Resolution Multispectral System

PicoMaster 100. Unprecedented finesse in creating 3D micro structures. UV direct laser writer for maskless lithography

Supplemental Method Information Zeiss LSM710

OPTIV CLASSIC 321 GL TECHNICAL DATA

Optical design of a high resolution vision lens

Bruker Optical Profilometer SOP Revision 2 01/04/16 Page 1 of 13. Bruker Optical Profilometer SOP

Nikon A1R. Multi-Photon & Laser Scanning Confocal Microscope. Kyle Marchuk Adam Fries Jordan Briscoe Kaitlin Corbin. April 2017.

DIC Imaging using Laser Scanning Microscopes (LSM) on Inverted Stands

Point Spread Function. Confocal Laser Scanning Microscopy. Confocal Aperture. Optical aberrations. Alternative Scanning Microscopy

CAMAG TLC VISUALIZER 2

TECHNICAL DATA. OPTIV CLASSIC 322 Version 3/2013

Nikon AZ100. Laser Scanning Macro Confocal Microscope. Jordan Briscoe Adam Fries Kyle Marchuk Kaitlin Corbin. May 2017.

Quality Performance, Innovative Design

Microscopic Structures

Quick Guide for Zeiss 710 Laser Scanning Confocal MGH Cancer Center

Confocal Application Notes Vol. 5 July 2010

Systems Biology. Optical Train, Köhler Illumination

Camera Overview. Olympus Digital Cameras for Materials Science Applications: For Clear and Precise Image Analysis. Digital Cameras for Microscopy

Point Autofocus Probe Surface Texture Measuring Instrument. PF-60 technical report

Operating Checklist for using the Laser Scanning Confocal Microscope. Leica TCS SP5.

A 3D Profile Parallel Detecting System Based on Differential Confocal Microscopy. Y.H. Wang, X.F. Yu and Y.T. Fei

Basics of confocal imaging (part I)

CNC Video Measuring System. CNC Video Measuring System NEXIV VMZ-R

MIF ZEISS VIOLET CONFOCAL ZEN 2009 PROTOCOL

Speed and Image Brightness uniformity of telecentric lenses

Applications of Optics


CELL PHONE PHOTOGRAPHY

WITec Alpha 300R Quick Operation Summary October 2018

Nikon. King s College London. Imaging Centre. N-SIM guide NIKON IMAGING KING S COLLEGE LONDON

TRAINING MANUAL. Multiphoton Microscopy LSM 510 META-NLO

LSM 710 Confocal Microscope Standard Operation Protocol

Zeta-300 3D OPTICAL PROFILER

Opterra II Multipoint Scanning Confocal Microscope. Innovation with Integrity

R I T. Title: Wyko RST Plus. Semiconductor & Microsystems Fabrication Laboratory Revision: A Rev Date: 05/23/06 1 SCOPE 2 REFERENCE DOCUMENTS

Topics. - How to calibrate the LSM scanner. - How to clean the microscope. - How to adjust the pinhole alignment. - How to adjust the Collimator

SHORT INSTRUCTIONS FOR OPERATING LSM1/2 (Zeiss LSM510) AT CIAN Version 1.4, September 2014

MEASUREMENT APPLICATION GUIDE OUTER/INNER

TECHNICAL DATA OPTIV CLASSIC 432

Transcription:

Systematic Workflow via Intuitive GUI Easy operation accomplishes your goals faster than ever. 16

With the LEXT OLS4100, observation or measurement begins immediately once the sample is placed on the stage. Thanks to our easy three-step Imaging, Measurement and Reports process, measurement procedures can be quickly mastered, even by those not familiar with laser microscopy. The OLS4100 s macro map function allows wide-field image display of a sample under low magnification, with a rectangular observation marker on the macro sample image. The field of view can be set up to 441 (21x21) times wider than the conventional view. When used with the motorized six-lens nosepiece, the macro map function allows smooth, convenient, oneclick operation for stage movement and magnification. Accurate parfocality and objective centering can be preset and synchronized with one-click stage movement and magnification. Two stitching methods are available for scanning large areas: Manual mode for live image acquisition and Automatic mode for faster image acquisition. Operation is quick and simple 2D stitching starts automatically at the touch of a single button, and wide area images are acquired immediately. The stitching size is available from five steps in 3x3, 5x5, 7x7, 9x9, and 21x21 in Automatic mode. Unnecessary parts of the acquired images can also be removed manually with simple mouse/joystick operation. 17

Speed of image acquisition is significantly increased, with automatic adjustments for brightness and position across the Z-axis direction and planar surface. Conventional 3D scanning requires complicated settings that are difficult for novice users. With the LEXT OLS4100 s new Smart Scan mode, even first-time users can quickly acquire 3D images with a single click of a button. In addition to upper and lower limit settings, appropriate brightness level is automatically set up by the system based on the image to be captured, allowing even new users to obtain accurate height measurements and an optimized image. Upper/Lower Limit Setting Acquisition Pitch Setting Brightness Setting Upper Limit Setting Auto Recognition Lower Limit Setting Lowered to Preset Level Automatic Control Brightness Control on a Plane Brightness Control with a Range of Height Lower Limit Setting Brightness Setting Upper Limit Setting Brightness & Upper/ Lower Limit Check Click on Acquisition Button Acquisition Click on Acquisition Button Auto Lower Limit Setting Auto Upper Limit Setting Auto Brightness Setting Acquisition 18

The new Ultra-Fast mode allows scanned image acquisition at twice the speed of conventional Fast mode, and approximately nine times the speed of Fine mode. This makes it possible to measure micro-samples with very steep angles, such as the tip of a knife, which is difficult to observe due to fine Z-step movement and high magnification. Fine 1.0 Fast 5.5 Ultra-Fast 9.3 0 2 4 6 8 10 (images) Actual scanning time varies depending on magnification and Z-acquisition range. The OLS4100 also comes with a Band Scan mode for measurement of limited target areas, providing measurement performance 1/8th faster than conventional modes. 1024 X 128 Acquisition with a Full Scan Acquisition with Band Scan (1/8th ) 19

As in macro mapping, the area to be observed can be specified from a wide area map. In Automatic mode, an area map can be automatically generated in roughly half the time it normally takes by setting a rectangular stitching size of up to 625 images. Observation can begin immediately once the target area is specified on the area map. Individual 2D Images Before Stitching (Simulation) 2D Image After Stitching 3D Image After Stitching Stitching Area: Square (21x3) 63 Pieces Stitching Area: Circle (3 Points) In Live mode, the area to be observed can be selected manually by tracing the required region onscreen. This is ideal when the sample has an irregular shape. In Smart Scan mode, all it takes is the click of a button. As the location across the Z-axis is automatically adjusted, image acquisition in the Z-axis direction can be restricted to required areas only, for rapid high-power observation across a wide area. Measuring Areas in Z-Axis Direction Smart Scan Mode Conventional Acquisition Mode 20

The OLS4100 generates reports at the touch of a button after measurement, and an edit function allows the operator to customize each report template. Copying and pasting measured results into a word processing/spreadsheet application is also quite simple, as is retrieving required images/reports from a database. A detailed user-designed wizard function eliminates the need for lengthy training and allows quick and easy operation by new operators. Image A Image B Image C Image D 21

Basic Principles of the LEXT OLS4100 The lateral resolution of an optical microscope is defined largely by the parameters of the optics and the wavelength of the light source. With a 405 nm short-wavelength semiconductor laser, the LEXT OLS4100 enjoys a high lateral resolution in comparison to a conventional microscope using visible light with a 550 nm peak. 400 nm 600 nm 700 nm 800 nm OLS4100 Light Source (405 nm) Conventional Microscopes (550 nm peak) For 2D scanning, the OLS4100 incorporates an Olympus scanner-on-scanner. An electromagnetic MEMS scanner handles the X direction, while a high-precision Galvano mirror takes care of scanning in the Y direction. This innovative system enables the axis of the scanner and the exit pupil of the objective to be placed at an optically conjugate position. This ideal optical layout allows accurate high-speed, low-distortion X-Y scanning, enabling the OLS4100 to provide high-density scanning up to 4096 x 4096 pixels. A confocal optical system captures only the in-focus image while simultaneously eliminating flare. In addition, confocal technology can be used as a height sensor since only thin image planes of the same height are captured. The OLS4100 is equipped with an Olympus dual confocal system, enhancing optical performance for precise 3D images even with samples made up of materials with different reflectances. The circular pinhole point of laser light also produces a uniform confocal effect, enhancing contrast in every direction. Non-Confocal Confocal Non-Confocal Confocal Photomultiplier Photomultiplier Circular Confocal Pinhole Telan Lens Telan Lens 22

Detects Peak Intensity for Every Pixel Reflective Intensity Calculated Reflection Intensity Calculated Height Height Obtaining height information is a primary function of the OLS4100 and is achieved by moving the objective upward to detect the change of light intensity along the Z-axis. Olympus CFO (calculated focus operation) technology detects light intensity automatically in order to obtain discrete height data. The approximate curve of an ideal I-Z curve is calculated alongside the maximum brightness value and Z-axis information, which define each image pixel. CFO search technology significantly improves repeatability one of the most indispensable assets of a measurement tool. 10-nanometer resolution in the Z-axis direction to enable 3D surface contour measurement Horizontal (X-Y direction) resolution of 0.12 μm to enable high-definition image observation Violet laser enables non-contact observation and measurement 23

Sample Applications 1 2 Wafer Bump (objective 100x/optical zoom 1.5x/scanning area 85 μm x 85 μm) 2 Light Guide Panel (objective 50x/optical zoom 1x/scanning area 256 μm x 256 μm) Chip Pad (objective 50x/optical zoom 2x/scanning area 128 μm x 128 μm) Laser Dot on Light Guide Panel (objective 100x/optical zoom 1x/scanning area 128 μm x 128 μm) 3 4 1 2 Photomask (objective 20x/optical zoom 1x/scanning area 640 μm x 640 μm) Sample provided by Koshibu Precision Co., Ltd. (P3,P24) 2 Micro Lens (objective 100x/optical zoom 1x/scanning area 128 μm x 128 μm) Flexible PCB Connector (objective 50x/optical zoom 1x/scanning area 256 μm x 256 μm) MEMS (objective 20x/optical zoom 1.3x/scanning area 483 μm x 483 μm) 3 4 24

1 2 Diamond Electrocoating Tool (objective 50x/optical zoom 1x/scanning area 256 μm x 256 μm) 2 Carbon (objective 100x/optical zoom 1x/scanning area 128 μm x 128 μm) Ultra-Thin Pipe (objective 100x/optical zoom 1x/scanning area 128 μm x 128 μm) Adhesive Tape (objective 50x/optical zoom 2x/scanning area 128 μm x 128 μm) 3 4 5 6 7 Sandpaper #400 (3D) (objective 20x/optical zoom 1x/scanning area 640 μm x 640 μm) 6 Sandpaper #400 (2D) (objective 20x/optical zoom 1x/scanning area 640 μm x 640 μm) 7 Super-Density Fabric (3D) (objective 20x/optical zoom 1x/scanning area 640 μm x 640 μm) 25

Standard-Type OLS4100-SAF MAIN UNIT DIMENSIONS 276 358 COMBINATION SYSTEM DIMENSIONS 550 405 300mm-Stage-Type OLS4100-LAF Unit: mm 448 1300 26

LSM Section Light Source/Detector Light Source: 405 nm Semiconductor Laser, Detector: Photomultiplier Total Magnification 108x 17,280x Zoom Optical Zoom: 1x 8x Measurement Planar Measurement Repeatability 100x: 3σn-1=0.02 µm Accuracy Measurement Value ±2% Height Measurement System Revolving Nosepiece Vertical-Drive System Stroke Scale Resolution Movement Resolution 10 mm 0.8 nm 10 nm Display Resolution 1 nm Repeatability 50x: σn-1=0.012 µm Accuracy 0.2+L/100 µm or Less (L=Measuring Length) Color Observation Section Light Source/Detector Light Source: White LED, Detector: 1/1.8-Inch 2-Megapixel Single-Panel CCD Zoom Digital Zoom: 1x 8x Revolving Nosepiece Differential Interference Contrast Unit Objective Z Focusing Unit Stroke XY Stage Motorized BF Sextuple Revolving Nosepiece Differential Interference Contrast Slider: U-DICR, Polarizing Plate Unit Built-In BF Plan Semi-apochromat 5x, 10x LEXT-Dedicated Plan Apochromat 20x, 50x, 100x 100 mm 100x100 mm (Motorized Stage), Option: 300x300 mm (Motorized Stage) This product is designed for use in industrial environments for the EMC performance. Using it in a residential environment may affect other equipment in the environment Model Magnification Field of View Working Distance (WD) Numerical Aperture (NA) MPLFLN5X 108x-864x 2,560-320 µm 20.0 mm 0.15 MPLFLN10X 216x-1,728x 1,280-160 µm 11.0 mm 0.30 MPLAPON20XLEXT 432x-3,456x 640-80 µm 1.0 mm 0.60 MPLAPON50XLEXT 1,080x-8,640x 256-32 µm 0.35 mm 0.95 MPLAPON100XLEXT 2,160x-17,280x 128-16 µm 0.35 mm 0.95 27

For enquiries - contact M1775E-102015